RLG00000035074

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000243
Physical Location & Seq
Reverse (-)
60345280 .. 60346263
984 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035074

Sequence Viewer

Length: 585 bp
ATGTTTGTTATTGATGGGGATGGGATTTTGGTTGTAGGTATCTTGCAATCTGAATCTTGCCAGCTTCTATTTTTCCTTGGATTTCTTAGCCAAGCCATTATTGTGCAGGTTGTTGAACCTGAAAATCCAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTAGGATGGTTCCATACCCTAATCTTGAATGGATGGCGGCATTATGGAAAATTATGAAGGGGGAGCCCCCTCTGGTTCCTGATTCTCTTTCAAGGGAGGCACAAGATTTTATCCATCTATGCTTGCAAGTTGAGCCAGATAATCGTCCCACCGCTGCTCAGCTTTTAAAGCATCCATTTGTAAATAAGCCCCTTCCTCAGTTCCTCCCACGTCTTCTGGATCAGTATCTCCTTACAATCACCACAGGCAGAGTTGAAAAGATTTATTCAGCTATAATTTGTGATGCTCTATTCGACCCAAAGAAGCAGGCTGACAACTCATCCATGTTCTATAATTTGCTTTGGAATCCTGTGTTCGGCCACCAAGTTTTGGCTTACTCTGGTAGAGTGCATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

195

Amino Acids

21.84

Weight (kDa)

5.1

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 41 - 123 4e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 48 - 119 2.7e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 97
AccB7I CCANNNNNTGG 2 cut(s) 134, 556
AciI CCGC 2 cut(s) 224, 339
AclWI GGATC 1 cut(s) 414
AcoI YGGCCR 1 cut(s) 544
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 3 cut(s) 134, 542, 556
AgsI TTSAA 4 cut(s) 116, 215, 279, 443
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 4 cut(s) 64, 146, 349, 458
AluI AGCT 4 cut(s) 64, 146, 349, 458
AlwI GGATC 1 cut(s) 414
AoxI GGCC 2 cut(s) 136, 544
ApeKI GCWGC 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 418
BanII GRGCYC 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 392
BbvI GCAGC 1 cut(s) 328
BccI CCATC 5 cut(s) 8, 14, 187, 214, 309
BciT130I CCWGG 1 cut(s) 129
BfaI CTAG 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 2 cut(s) 225, 342
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 2 cut(s) 226, 343
Bme1390I CCNGG 1 cut(s) 129
BmgBI CACGTC 1 cut(s) 398
BmiI GGNNCC 3 cut(s) 198, 252, 264
BmrFI CCNGG 1 cut(s) 129
BmsI GCATC 2 cut(s) 367, 460
BpiI GAAGAC 1 cut(s) 392
Bpu1102I GCTNAGC 1 cut(s) 345
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 76
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 542, 556
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 6 cut(s) 25, 170, 198, 225, 358, 506
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 3 cut(s) 134, 542, 556
BseMII CTCAG 2 cut(s) 359, 398
BseXI GCAGC 1 cut(s) 328
BsgI GTGCAG 1 cut(s) 125
BshFI GGCC 2 cut(s) 138, 546
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 3 cut(s) 134, 542, 556
BsmFI GGGAC 1 cut(s) 318
BsnI GGCC 2 cut(s) 138, 546
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 1 cut(s) 406
Bsp1720I GCTNAGC 1 cut(s) 345
BspACI CCGC 2 cut(s) 224, 339
BspANI GGCC 2 cut(s) 138, 546
BspCNI CTCAG 2 cut(s) 358, 397
BspLI GGNNCC 3 cut(s) 198, 252, 264
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 1 cut(s) 414
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 1 cut(s) 406
BssT1I CCWWGG 1 cut(s) 76
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 172
BstC8I GCNNGC 4 cut(s) 62, 311, 495, 579
BstDEI CTNAG 3 cut(s) 86, 345, 384
BstF5I GGATG 6 cut(s) 25, 170, 198, 225, 358, 506
BstKTI GATC 1 cut(s) 409
BstMBI GATC 1 cut(s) 406
BstMWI GCNNNNNNNGC 2 cut(s) 319, 355
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 581
BstSCI CCNGG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 392
BsuRI GGCC 2 cut(s) 138, 546
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 6 cut(s) 25, 170, 198, 225, 358, 506
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 4 cut(s) 62, 311, 495, 579
Csp6I GTAC 1 cut(s) 168
CviAII CATG 2 cut(s) 511, 578
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 3 cut(s) 86, 345, 384
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
DraI TTTAAA 1 cut(s) 354
EaeI YGGCCR 1 cut(s) 544
Eco130I CCWWGG 1 cut(s) 76
Eco24I GRGCYC 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 76
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 76
FaeI CATG 2 cut(s) 514, 581
FaqI GGGAC 1 cut(s) 318
FatI CATG 2 cut(s) 510, 577
Fnu4HI GCNGC 2 cut(s) 225, 342
FokI GGATG 6 cut(s) 32, 177, 205, 232, 345, 493
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 2 cut(s) 225, 342
FspBI CTAG 1 cut(s) 189
GluI GCNGC 2 cut(s) 225, 342
HaeIII GGCC 2 cut(s) 138, 546
Hin1II CATG 2 cut(s) 514, 581
HincII GTYRAC 1 cut(s) 186
HindII GTYRAC 1 cut(s) 186
HinfI GANTC 4 cut(s) 53, 157, 269, 532
HpaI GTTAAC 1 cut(s) 186
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 1 cut(s) 186
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 3 cut(s) 212, 266, 404
Hpy8I GTNNAC 1 cut(s) 186
HpyAV CCTTC 3 cut(s) 149, 238, 389
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 4 cut(s) 46, 106, 313, 577
HpyF10VI GCNNNNNNNGC 2 cut(s) 319, 355
HpyF3I CTNAG 3 cut(s) 86, 345, 384
HpySE526I ACGT 1 cut(s) 397
Hsp92II CATG 2 cut(s) 514, 581
KspAI GTTAAC 1 cut(s) 186
Kzo9I GATC 1 cut(s) 406
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 2 cut(s) 367, 460
MaeI CTAG 1 cut(s) 189
MaeII ACGT 1 cut(s) 397
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MboII GAAGA 1 cut(s) 392
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 3 cut(s) 237, 462, 520
MlyI GAGTC 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 4 cut(s) 267, 277, 393, 401
MseI TTAA 2 cut(s) 185, 353
MslI CAYNNNNRTG 1 cut(s) 101
MspA1I CMGCKG 2 cut(s) 146, 341
MspR9I CCNGG 1 cut(s) 129
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 319, 355
NdeII GATC 1 cut(s) 406
NlaIII CATG 2 cut(s) 514, 581
NlaIV GGNNCC 3 cut(s) 198, 252, 264
NspI RCATGY 1 cut(s) 581
PaeI GCATGC 1 cut(s) 581
PfeI GAWTC 3 cut(s) 53, 269, 532
PflMI CCANNNNNTGG 2 cut(s) 134, 556
PkrI GCNGC 2 cut(s) 226, 343
PleI GAGTC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 3 cut(s) 198, 252, 264
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 2 cut(s) 185, 353
SatI GCNGC 2 cut(s) 225, 342
Sau3AI GATC 1 cut(s) 406
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 1 cut(s) 255
SetI ASST 9 cut(s) 40, 66, 111, 121, 133, 148, 351, 400, 460
SfaNI GCATC 2 cut(s) 367, 460
SmiMI CAYNNNNRTG 1 cut(s) 101
SphI GCATGC 1 cut(s) 581
Sse9I AATT 3 cut(s) 237, 462, 520
SsiI CCGC 2 cut(s) 224, 339
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 127
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 400
TaqI TCGA 1 cut(s) 480
TasI AATT 3 cut(s) 237, 462, 520
TatI WGTACW 1 cut(s) 167
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 3 cut(s) 53, 269, 532
Tru1I TTAA 2 cut(s) 185, 353
Tru9I TTAA 2 cut(s) 185, 353
TseI GCWGC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 257
Van91I CCANNNNNTGG 2 cut(s) 134, 556
XceI RCATGY 1 cut(s) 581
XcmI CCANNNNNNNNNTGG 1 cut(s) 150
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.