AT4G08500

Mitogen-Activated Protein Kinase Kinase Kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000243
Physical Location & Seq
Reverse (-)
5403671 .. 5407320
3650 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08500.1

Sequence Viewer

Length: 1827 bp
ATGGACAGAATTCTAGCTCGTATGAAGAAATCAACTGGACGAAGAGGAGGAGATAAGAATATTACTCCGGTACGGCGGTTAGAGCGTCGCGATGCGGCGAGGAATATCAATTACGACGCAGCTTCATGTTCTAGTTCGTCAGCTGAAGATCTTTCCGTTTCGACTTCTTCGTTGATGACTCGCTCTTTGGAGTTTCCGGAGCCTACTAGTTTCCGAATCGGTGGTGGTGTTGGAGAGATGGATCGGATTTATCGGTCTCTTGGTGTTTCTGGTCCTGATGATTTGGCTATTTCTTTTGATGCTTGGGAAGCTTGTAAGAAACGTTCTTCTTCAGATGTTGTTAATAGGTTTAAGTCTTTTGATCTTGATAAGGTTCGTGATCAGGATTTGAGTGAAGAAGGTCCTAGTGGTGTTGTTGTTGGTTCTGATTCAATGAATCATAAGGTTCAGGGTCAGGATTTGAGTGAAGCAGGTCCTAGTGGTGGAATTGTTACTGAGTTGAGTGAGATTGGGAATTTAATCACTCCTGTGGATAGGTTAGTAGCTGATGGTGTGGTAGAGAATAGGCGTGTTATGGAAAGAACACCAACTATTGTGAAGTCGAAAGGGTATCTTGTACCAAATAATGTAGTGGCTGTTGGTGTTGGTGTTGGTGGTGGTATTAAGGGGCTAAGACCACCAGTACTTAAGCCTCCTCCGGCTATGAAACGACCCCCTATTGATCATCGGGGATCGTCTTGGGATTTCCTGACGCATTTCGCTCCAAGTGAAACAGTTAAGCGGCCGAGTTCCTCTTCTTCTTCTTCCGAGGATGGATGCGATGAAGAGGAAGGCAAGGAAGAGGAAGCGGAAGCGGAAGAGATGGGAGCTAGGTTTATCCAGTTGGGGGATACGGCTGACGAGACGTGCTCATTCACTACAAATGAGGGTGACTCCTCAAGCACAGTATCCAATACTTCGCCTATCTATCCAGATGGAGGAGCTATCATAACGTCTTGGCAAAAGGGTCAACTTTTGGGACGAGGATCATTTGGTTCTGTGTATGAAGGCATTTCTGGAGATGGGGACTTCTTTGCTGTCAAGGAAGTTTCACTTCTTGATCAGGGAAGTCAGGCACAAGAATGCATACAACAACTTGAGGGGGAGATTAAACTACTTAGTCAGCTTCAGCATCAGAATATTGTGAGATATCGTGGCACAGCCAAGGATGGGTCAAACTTGTACATTTTTCTTGAGCTTGTAACCCAAGGGTCCCTTCTAAAACTCTACCAAAGATACCAGCTTCGGGACTCTGTAGTCTCCTTGTACACTAGACAGATTCTTGACGGTTTGAAATATCTCCACGATAAAGGTTTTATTCACAGGGACATCAAATGTGCAAATATATTGGTGGACGCTAATGGCGCCGTCAAACTTGCAGATTTTGGATTGGCAAAGGTTTCAAAGTTTAACGACATTAAGTCCTGCAAGGGAACTCCATTTTGGATGGCTCCAGAGGTTATTAACCGAAAGGATAGTGATGGCTATGGAAGTCCAGCTGATATATGGAGCCTCGGGTGCACTGTGCTGGAAATGTGTACTGGTCAGATCCCCTACTCTGATCTCGAACCCGTTCAAGCCCTGTTTAGGATCGGAAGGGGTACTCTTCCGGAAGTACCTGATACGTTATCACTAGATGCTCGGCTTTTCATACTTAAGTGTCTTAAAGTGAACCCGGAAGAGCGGCCAACTGCAGCTGAACTGCTGAACCATCCATTTGTGAGAAGACCCTTACCATCCGTGGGTTCAGGAGGCTCGGGATCAGCATCTCCCCTTCTCCGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

608

Amino Acids

66.02

Weight (kDa)

5.49

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 333 - 587 2.2e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 336 - 583 2.8e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1295
Acc36I ACCTGC 1 cut(s) 461
AccB1I GGYRCC 1 cut(s) 1403
AccBSI CCGCTC 1 cut(s) 1723
AccII CGCG 1 cut(s) 90
AccIII TCCGGA 2 cut(s) 196, 1648
AciI CCGC 6 cut(s) 76, 95, 781, 848, 854, 1723
AclI AACGTT 1 cut(s) 322
AclWI GGATC 6 cut(s) 249, 739, 1033, 1582, 1637, 1807
AcoI YGGCCR 2 cut(s) 782, 1724
AcsI RAATTY 2 cut(s) 9, 514
AcuI CTGAAG 3 cut(s) 165, 315, 1151
AcyI GRCGYC 1 cut(s) 1404
AfaI GTAC 8 cut(s) 72, 618, 684, 1223, 1307, 1579, 1642, 1656
AfiI CCNNNNNNNGG 7 cut(s) 482, 886, 977, 1209, 1285, 1626, 1781
AflII CTTAAG 2 cut(s) 686, 1694
AgsI TTSAA 4 cut(s) 432, 1333, 1443, 1616
AhdI GACNNNNNGTC 1 cut(s) 1459
AhlI ACTAGT 1 cut(s) 206
AjiI CACGTC 1 cut(s) 906
AjuI GAANNNNNNNTTGG 4 cut(s) 1239, 1271, 1465, 1497
AleI CACNNNNGTG 1 cut(s) 527
Alw21I GWGCWC 2 cut(s) 911, 1562
Alw26I GTCTC 3 cut(s) 261, 896, 1303
Alw44I GTGCAC 1 cut(s) 1558
AlwI GGATC 6 cut(s) 249, 739, 1033, 1582, 1637, 1807
Ama87I CYCGRG 2 cut(s) 1553, 1795
Aor13HI TCCGGA 2 cut(s) 196, 1648
AoxI GGCC 2 cut(s) 782, 1724
ApaLI GTGCAC 1 cut(s) 1558
ApeKI GCWGC 2 cut(s) 119, 1733
ApoI RAATTY 2 cut(s) 9, 514
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
Asp700I GAANNNNTTC 1 cut(s) 1611
AspLEI GCGC 1 cut(s) 1406
AspS9I GGNCC 4 cut(s) 272, 401, 473, 1251
AsuC2I CCSGG 1 cut(s) 1715
AsuHPI GGTGA 1 cut(s) 941
AvaI CYCGRG 2 cut(s) 1553, 1795
AvaII GGWCC 4 cut(s) 272, 401, 473, 1251
BaeGI GKGCMC 1 cut(s) 1562
BaeI ACNNNNGTAYC 2 cut(s) 62, 95
BanI GGYRCC 1 cut(s) 1403
BbsI GAAGAC 1 cut(s) 1771
Bbv12I GWGCWC 2 cut(s) 911, 1562
BbvI GCAGC 2 cut(s) 131, 1745
BceAI ACGGC 3 cut(s) 89, 909, 1391
BciVI GTATCC 2 cut(s) 883, 958
BclI TGATCA 3 cut(s) 379, 721, 1099
BcnI CCSGG 1 cut(s) 1715
BcoDI GTCTC 3 cut(s) 261, 896, 1303
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 8 cut(s) 14, 132, 207, 405, 477, 870, 1311, 1673
BfmI CTRYAG 2 cut(s) 1293, 1731
BfoI RGCGCY 1 cut(s) 1407
BfrI CTTAAG 2 cut(s) 686, 1694
BfuAI ACCTGC 1 cut(s) 461
BfuI GTATCC 2 cut(s) 883, 958
BglII AGATCT 1 cut(s) 148
BisI GCNGC 5 cut(s) 96, 120, 782, 1724, 1734
BlsI GCNGC 5 cut(s) 97, 121, 783, 1725, 1735
BmcAI AGTACT 1 cut(s) 684
Bme1390I CCNGG 1 cut(s) 1715
Bme18I GGWCC 4 cut(s) 272, 401, 473, 1251
BmeRI GACNNNNNGTC 1 cut(s) 1459
BmeT110I CYCGRG 2 cut(s) 1553, 1795
BmgBI CACGTC 1 cut(s) 906
BmgT120I GGNCC 4 cut(s) 272, 401, 473, 1251
BmiI GGNNCC 6 cut(s) 201, 1252, 1253, 1405, 1491, 1550
BmrFI CCNGG 1 cut(s) 1715
BmsI GCATC 6 cut(s) 82, 289, 806, 1180, 1666, 1814
BpiI GAAGAC 1 cut(s) 1771
BplI GAGNNNNNCTC 4 cut(s) 893, 925, 917, 949
BpmI CTGGAG 2 cut(s) 1077, 1476
BpuEI CTTGAG 3 cut(s) 922, 1157, 1253
BpuMI CCSGG 1 cut(s) 1715
BsaBI GATNNNNATC 1 cut(s) 1804
BsaHI GRCGYC 1 cut(s) 1404
BsaI GGTCTC 1 cut(s) 261
BsaJI CCNNGG 5 cut(s) 807, 1203, 1246, 1552, 1779
BsaWI WCCGGW 3 cut(s) 67, 196, 1648
Bsc4I CCNNNNNNNGG 7 cut(s) 482, 886, 977, 1209, 1285, 1626, 1781
Bse1I ACTGG 4 cut(s) 40, 680, 880, 1585
Bse8I GATNNNNATC 1 cut(s) 1804
BseAI TCCGGA 2 cut(s) 196, 1648
BseDI CCNNGG 5 cut(s) 807, 1203, 1246, 1552, 1779
BseGI GGATG 6 cut(s) 817, 821, 1213, 1491, 1750, 1775
BseJI GATNNNNATC 1 cut(s) 1804
BseLI CCNNNNNNNGG 7 cut(s) 482, 886, 977, 1209, 1285, 1626, 1781
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 4 cut(s) 40, 680, 880, 1585
BseRI GAGGAG 5 cut(s) 60, 63, 684, 925, 993
BseSI GKGCMC 1 cut(s) 1562
BseX3I CGGCCG 1 cut(s) 782
BseXI GCAGC 2 cut(s) 131, 1745
Bsh1236I CGCG 1 cut(s) 90
Bsh1285I CGRYCG 1 cut(s) 785
BshFI GGCC 2 cut(s) 784, 1726
BshNI GGYRCC 1 cut(s) 1403
BsiEI CGRYCG 1 cut(s) 785
BsiHKAI GWGCWC 2 cut(s) 911, 1562
BsiHKCI CYCGRG 2 cut(s) 1553, 1795
BsiSI CCGG 5 cut(s) 68, 197, 698, 1649, 1715
BslFI GGGAC 5 cut(s) 1032, 1079, 1237, 1301, 1379
BslI CCNNNNNNNGG 7 cut(s) 482, 886, 977, 1209, 1285, 1626, 1781
BsmAI GTCTC 3 cut(s) 261, 896, 1303
BsmBI CGTCTC 1 cut(s) 896
BsmFI GGGAC 5 cut(s) 1032, 1079, 1237, 1301, 1379
BsmI GAATGC 1 cut(s) 1127
BsnI GGCC 2 cut(s) 784, 1726
Bso31I GGTCTC 1 cut(s) 261
BsoBI CYCGRG 2 cut(s) 1553, 1795
Bsp1286I GDGCHC 2 cut(s) 911, 1562
Bsp13I TCCGGA 2 cut(s) 196, 1648
Bsp1407I TGTACA 2 cut(s) 1221, 1305
Bsp68I TCGCGA 1 cut(s) 90
BspACI CCGC 6 cut(s) 76, 95, 781, 848, 854, 1723
BspANI GGCC 2 cut(s) 784, 1726
BspCNI CTCAG 1 cut(s) 487
BspEI TCCGGA 2 cut(s) 196, 1648
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 6 cut(s) 201, 1252, 1253, 1405, 1491, 1550
BspMAI CTGCAG 1 cut(s) 1735
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 6 cut(s) 249, 739, 1033, 1582, 1637, 1807
BspQI GCTCTTC 1 cut(s) 1713
BspT107I GGYRCC 1 cut(s) 1403
BspTI CTTAAG 2 cut(s) 686, 1694
BspTNI GGTCTC 1 cut(s) 261
BsrBI CCGCTC 1 cut(s) 1723
BsrGI TGTACA 2 cut(s) 1221, 1305
BsrI ACTGG 4 cut(s) 40, 680, 880, 1585
BssECI CCNNGG 5 cut(s) 807, 1203, 1246, 1552, 1779
BssNI GRCGYC 1 cut(s) 1404
BssT1I CCWWGG 2 cut(s) 1203, 1246
Bst4CI ACNGT 4 cut(s) 775, 946, 1328, 1564
Bst6I CTCTTC 7 cut(s) 37, 799, 819, 834, 852, 1650, 1713
BstACI GRCGYC 1 cut(s) 1404
BstAFI CTTAAG 2 cut(s) 686, 1694
BstAUI TGTACA 2 cut(s) 1221, 1305
BstDEI CTNAG 3 cut(s) 495, 671, 1157
BstDSI CCRYGG 1 cut(s) 1779
BstF5I GGATG 6 cut(s) 817, 821, 1213, 1491, 1750, 1775
BstFNI CGCG 1 cut(s) 90
BstH2I RGCGCY 1 cut(s) 1407
BstHHI GCGC 1 cut(s) 1406
BstMAI GTCTC 3 cut(s) 261, 896, 1303
BstMCI CGRYCG 1 cut(s) 785
BstMWI GCNNNNNNNGC 4 cut(s) 82, 308, 1403, 1557
BstSCI CCNGG 1 cut(s) 1713
BstSFI CTRYAG 2 cut(s) 1293, 1731
BstSLI GKGCMC 1 cut(s) 1562
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 2 cut(s) 131, 1745
BstV2I GAAGAC 1 cut(s) 1771
BstX2I RGATCY 2 cut(s) 148, 1587
BstYI RGATCY 2 cut(s) 148, 1587
BstZI CGGCCG 1 cut(s) 782
BsuI GTATCC 2 cut(s) 883, 958
BsuRI GGCC 2 cut(s) 784, 1726
BtgI CCRYGG 1 cut(s) 1779
BtgZI GCGATG 2 cut(s) 105, 834
BtrI CACGTC 1 cut(s) 906
BtsCI GGATG 6 cut(s) 817, 821, 1213, 1491, 1750, 1775
BtsIMutI CAGTG 1 cut(s) 1560
BtuMI TCGCGA 1 cut(s) 90
BveI ACCTGC 1 cut(s) 461
CfoI GCGC 1 cut(s) 1406
Cfr13I GGNCC 4 cut(s) 272, 401, 473, 1251
CseI GACGC 4 cut(s) 74, 125, 760, 1403
Csp6I GTAC 8 cut(s) 71, 617, 683, 1222, 1306, 1578, 1641, 1655
CviAII CATG 1 cut(s) 126
CviQI GTAC 8 cut(s) 71, 617, 683, 1222, 1306, 1578, 1641, 1655
DdeI CTNAG 3 cut(s) 495, 671, 1157
DinI GGCGCC 1 cut(s) 1405
DrdI GACNNNNNNGTC 1 cut(s) 1295
DriI GACNNNNNGTC 1 cut(s) 1459
DseDI GACNNNNNNGTC 1 cut(s) 1295
EaeI YGGCCR 2 cut(s) 782, 1724
EagI CGGCCG 1 cut(s) 782
Eam1104I CTCTTC 7 cut(s) 37, 799, 819, 834, 852, 1650, 1713
Eam1105I GACNNNNNGTC 1 cut(s) 1459
EarI CTCTTC 7 cut(s) 37, 799, 819, 834, 852, 1650, 1713
EclXI CGGCCG 1 cut(s) 782
Eco130I CCWWGG 2 cut(s) 1203, 1246
Eco31I GGTCTC 1 cut(s) 261
Eco32I GATATC 1 cut(s) 1190
Eco47I GGWCC 4 cut(s) 272, 401, 473, 1251
Eco52I CGGCCG 1 cut(s) 782
Eco57I CTGAAG 3 cut(s) 165, 315, 1151
Eco88I CYCGRG 2 cut(s) 1553, 1795
EcoO109I RGGNCCY 3 cut(s) 401, 473, 1251
EcoRI GAATTC 1 cut(s) 9
EcoRV GATATC 1 cut(s) 1190
EcoT14I CCWWGG 2 cut(s) 1203, 1246
EcoT22I ATGCAT 1 cut(s) 1127
EgeI GGCGCC 1 cut(s) 1405
EheI GGCGCC 1 cut(s) 1405
ErhI CCWWGG 2 cut(s) 1203, 1246
Esp3I CGTCTC 1 cut(s) 896
FaeI CATG 1 cut(s) 129
FalI AAGNNNNNCTT 6 cut(s) 597, 629, 1077, 1109, 1239, 1271
FaqI GGGAC 5 cut(s) 1032, 1079, 1237, 1301, 1379
FatI CATG 1 cut(s) 125
FbaI TGATCA 3 cut(s) 379, 721, 1099
Fnu4HI GCNGC 5 cut(s) 96, 120, 782, 1724, 1734
FokI GGATG 6 cut(s) 824, 828, 1220, 1498, 1737, 1762
Fsp4HI GCNGC 5 cut(s) 96, 120, 782, 1724, 1734
FspBI CTAG 8 cut(s) 14, 132, 207, 405, 477, 870, 1311, 1673
GlaI GCGC 1 cut(s) 1405
GluI GCNGC 5 cut(s) 96, 120, 782, 1724, 1734
GsuI CTGGAG 2 cut(s) 1077, 1476
HaeII RGCGCY 1 cut(s) 1407
HaeIII GGCC 2 cut(s) 784, 1726
HapII CCGG 5 cut(s) 68, 197, 698, 1649, 1715
HgaI GACGC 4 cut(s) 74, 125, 760, 1403
HhaI GCGC 1 cut(s) 1406
Hin1I GRCGYC 1 cut(s) 1404
Hin1II CATG 1 cut(s) 129
Hin6I GCGC 1 cut(s) 1404
HinP1I GCGC 1 cut(s) 1404
HincII GTYRAC 1 cut(s) 1010
HindII GTYRAC 1 cut(s) 1010
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 7 cut(s) 178, 216, 428, 436, 932, 1289, 1318
HpaII CCGG 5 cut(s) 68, 197, 698, 1649, 1715
HphI GGTGA 1 cut(s) 941
Hpy166II GTNNAC 6 cut(s) 1010, 1308, 1393, 1560, 1578, 1711
Hpy188I TCNGA 9 cut(s) 215, 246, 334, 427, 808, 1176, 1587, 1600, 1634
Hpy8I GTNNAC 6 cut(s) 1010, 1308, 1393, 1560, 1578, 1711
Hpy99I CGWCG 2 cut(s) 90, 119
HpyAV CCTTC 6 cut(s) 392, 824, 1040, 1265, 1629, 1823
HpyCH4III ACNGT 4 cut(s) 775, 946, 1328, 1564
HpyCH4IV ACGT 4 cut(s) 322, 905, 992, 1664
HpyCH4V TGCA 6 cut(s) 1125, 1379, 1418, 1467, 1560, 1733
HpyF10VI GCNNNNNNNGC 4 cut(s) 82, 308, 1403, 1557
HpyF3I CTNAG 3 cut(s) 495, 671, 1157
HpySE526I ACGT 4 cut(s) 322, 905, 992, 1664
Hsp92I GRCGYC 1 cut(s) 1404
Hsp92II CATG 1 cut(s) 129
HspAI GCGC 1 cut(s) 1404
KasI GGCGCC 1 cut(s) 1403
KflI GGGWCCC 1 cut(s) 1251
Kpn2I TCCGGA 2 cut(s) 196, 1648
Ksp22I TGATCA 3 cut(s) 379, 721, 1099
LguI GCTCTTC 1 cut(s) 1713
LmnI GCTCC 6 cut(s) 199, 766, 866, 980, 1495, 1548
Lsp1109I GCAGC 2 cut(s) 131, 1745
LweI GCATC 6 cut(s) 82, 289, 806, 1180, 1666, 1814
MaeI CTAG 8 cut(s) 14, 132, 207, 405, 477, 870, 1311, 1673
MaeII ACGT 4 cut(s) 322, 905, 992, 1664
MaeIII GTNAC 3 cut(s) 490, 929, 1240
MbiI CCGCTC 1 cut(s) 1723
MflI RGATCY 2 cut(s) 148, 1587
MhlI GDGCHC 2 cut(s) 911, 1562
MluCI AATT 4 cut(s) 9, 109, 486, 514
Mly113I GGCGCC 1 cut(s) 1404
MlyI GAGTC 3 cut(s) 172, 926, 1283
MmeI TCCRAC 1 cut(s) 211
Mph1103I ATGCAT 1 cut(s) 1127
MroI TCCGGA 2 cut(s) 196, 1648
MroXI GAANNNNTTC 1 cut(s) 1611
MslI CAYNNNNRTG 2 cut(s) 527, 1120
MspA1I CMGCKG 3 cut(s) 143, 1538, 1736
MspCI CTTAAG 2 cut(s) 686, 1694
MspI CCGG 5 cut(s) 68, 197, 698, 1649, 1715
MspR9I CCNGG 1 cut(s) 1715
Mva1269I GAATGC 1 cut(s) 1127
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 4 cut(s) 82, 308, 1403, 1557
NarI GGCGCC 1 cut(s) 1404
NciI CCSGG 1 cut(s) 1715
NlaIII CATG 1 cut(s) 129
NlaIV GGNNCC 6 cut(s) 201, 1252, 1253, 1405, 1491, 1550
NmeAIII GCCGAG 2 cut(s) 810, 1660
NmuCI GTSAC 1 cut(s) 929
NruI TCGCGA 1 cut(s) 90
NsiI ATGCAT 1 cut(s) 1127
OliI CACNNNNGTG 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 1713
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PctI GAATGC 1 cut(s) 1127
PdmI GAANNNNTTC 1 cut(s) 1611
PfeI GAWTC 4 cut(s) 216, 428, 436, 1318
PkrI GCNGC 5 cut(s) 97, 121, 783, 1725, 1735
PleI GAGTC 3 cut(s) 172, 926, 1283
PluTI GGCGCC 1 cut(s) 1407
PpsI GAGTC 3 cut(s) 172, 926, 1283
PpuMI RGGWCCY 3 cut(s) 401, 473, 1251
Psp1406I AACGTT 1 cut(s) 322
Psp5II RGGWCCY 3 cut(s) 401, 473, 1251
PspN4I GGNNCC 6 cut(s) 201, 1252, 1253, 1405, 1491, 1550
PspPI GGNCC 4 cut(s) 272, 401, 473, 1251
PspPPI RGGWCCY 3 cut(s) 401, 473, 1251
PsrI GAACNNNNNNTAC 2 cut(s) 307, 339
PstI CTGCAG 1 cut(s) 1735
PsuI RGATCY 2 cut(s) 148, 1587
PvuII CAGCTG 3 cut(s) 143, 1538, 1736
RruI TCGCGA 1 cut(s) 90
RsaI GTAC 8 cut(s) 72, 618, 684, 1223, 1307, 1579, 1642, 1656
RsaNI GTAC 8 cut(s) 71, 617, 683, 1222, 1306, 1578, 1641, 1655
RseI CAYNNNNRTG 2 cut(s) 527, 1120
SapI GCTCTTC 1 cut(s) 1713
SatI GCNGC 5 cut(s) 96, 120, 782, 1724, 1734
Sau96I GGNCC 4 cut(s) 272, 401, 473, 1251
ScaI AGTACT 1 cut(s) 684
SchI GAGTC 3 cut(s) 172, 926, 1283
ScrFI CCNGG 1 cut(s) 1715
SduI GDGCHC 2 cut(s) 911, 1562
SfaNI GCATC 6 cut(s) 82, 289, 806, 1180, 1666, 1814
SfcI CTRYAG 2 cut(s) 1293, 1731
SfoI GGCGCC 1 cut(s) 1405
SinI GGWCC 4 cut(s) 272, 401, 473, 1251
SmiMI CAYNNNNRTG 2 cut(s) 527, 1120
SmlI CTYRAG 5 cut(s) 686, 937, 1136, 1232, 1694
SmoI CTYRAG 5 cut(s) 686, 937, 1136, 1232, 1694
SpeI ACTAGT 1 cut(s) 206
Sse9I AATT 4 cut(s) 9, 109, 486, 514
SsiI CCGC 6 cut(s) 76, 95, 781, 848, 854, 1723
SspDI GGCGCC 1 cut(s) 1403
SspI AATATT 2 cut(s) 61, 1180
SspMI CTAG 8 cut(s) 14, 132, 207, 405, 477, 870, 1311, 1673
StyD4I CCNGG 1 cut(s) 1713
StyI CCWWGG 2 cut(s) 1203, 1246
TaaI ACNGT 4 cut(s) 775, 946, 1328, 1564
TaiI ACGT 4 cut(s) 325, 908, 995, 1667
TaqI TCGA 3 cut(s) 161, 602, 1605
TaqII GACCGA 1 cut(s) 243
TasI AATT 4 cut(s) 9, 109, 486, 514
TatI WGTACW 4 cut(s) 682, 1221, 1305, 1577
TauI GCSGC 3 cut(s) 98, 784, 1726
TfiI GAWTC 4 cut(s) 216, 428, 436, 1318
TscAI CASTG 1 cut(s) 1567
TseFI GTSAC 1 cut(s) 929
TseI GCWGC 2 cut(s) 119, 1733
Tsp45I GTSAC 1 cut(s) 929
TspDTI ATGAA 7 cut(s) 38, 114, 449, 719, 837, 1059, 1678
TspGWI ACGGA 3 cut(s) 145, 1768, 1808
TspRI CASTG 1 cut(s) 1567
Vha464I CTTAAG 2 cut(s) 686, 1694
VneI GTGCAC 1 cut(s) 1558
VpaK11BI GGWCC 4 cut(s) 272, 401, 473, 1251
XapI RAATTY 2 cut(s) 9, 514
XcmI CCANNNNNNNNNTGG 1 cut(s) 1542
XmnI GAANNNNTTC 1 cut(s) 1611
XspI CTAG 8 cut(s) 14, 132, 207, 405, 477, 870, 1311, 1673
ZrmI AGTACT 1 cut(s) 684
Zsp2I ATGCAT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.