Rw5G048280

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000243
Physical Location & Seq
Reverse (-)
83879406 .. 83884961
5556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048280.1

Sequence Viewer

Length: 2022 bp
ATGGAACTCCCGCCTGGAGGTGGTGTTGCCGCCGGGCCGGTTGCTTCTTCCTCTTCTTCATCATCACATGTTCAGTGGAAGTATGATTCACCTGCTCAGTGGAAGTATGATGTGTTCTTGAGTTTCAGGGGTCCAGACACTCGAAGGAGTATCACAGCTTATCTATACTATCGACTGAAGAGGAGGGGAATTAAAACATTCATGGATGATCCAGATCTTCAAGTAGGGGATGTGATTTCTCCCGCTCTCCTAAAGGCAATTGAAGGATCAAGGTTTGCAATTGTTGTTCTGTCGGAAAACTATGCTCATTCTACTTGGTGTTTGGAGGAACTTACAAAGATTTGTGAGTGCATGGAAGGAAGCAAAAGAATTTTCCCACTTTTTTATAAGGTGGAACCATCTGATGTACGGTACCAAACGAAGAGTTTCGGAGAAGCTTTTAATAAGCATGCAAGCTATGGGCGACACACATTACAGAATGTGCGGCGGTGGAATGATGCTTTAAACAAAGTGTCCAGTTTCTCTGGGTGGCATACAAAGAATTATAAGACTGATATAGAACTTGTAAACGCAATTGTGGATTTTGTGTGCAATAAGATACAACCTATTGACACTGAGTTTACAATGTCTTCCGGAGATTTTGAAGCATATGAAGCAACAAGAAAAGCCATAGATGAGGTTATGAAGGTGCTAAAAGATAAAGATGACGAGGTCACTGTGGATGGTGTCTACGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAAACACGTCGCTGCACAATCCTGCAAAAATGGGACTTCTCATCATGTGATTATGGCTGTTGTATCCCAGAGCCCTGACTTGACAAAAATTCAAGGCACATTGGCGGGGCCGAGCGAGTGTTGTACTGCGAATAGTAGTTGTAGTGCAAGTGTCGGAATTAAGGGAGTGCGGCTGCCGGGGCTGAAGCGGCCGCCGTGGATGGTGAGGGTTCCGGTGATTGACAATGGATGCTCGACTTGGGACATCGTGAGGGACTTCGCGCCGGAAGAAGAGAGGGAGTCGTCGGAAATGGTGCAGAGAAGGTTTGGTTCTTTTTCCTCGTCTTCTTCTGATGAAGAAGAAGTTGAAGAAGGAGAGGATGTTGACTTTTCCAGCACTATCACAGAGTTTTCCAGTATTTCTCCATATGGAAGGTTTTCGCCGAATGGGAAGCCTAAACTTATTGTCACTCATTGGGAAAAGGGTGATTTTCTCGGCAGCGGCTCTTTTGGGTCTGTGTATGAAGGAATTTCTGATGGCGGATACTTCATTGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAAGGCTGGGGAGGCAAAGAGTTTTTCAACTTGAACAGGAGATTGCTCTTCTGAGTCGGTTTGAGCATGAGAACATTGTTCAGTATTATGGCACACAAAAGGATGAATCAAAGCTCTATATCTTTCTGGAACTTGTAACTAAAGGCACCCTTCTAAAGCTATATCAAACGTACCATCTTACAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCGAAAAGTGATTCACAGGGACATTAAATGCGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCTGACTTTGGATTGGCAAAGACTATCAAAATGAACAACATAAAATCTTGCCAAGGAACTGCATACTGGATGGCCCCTGAGGTTGTTAATAGTAAGAGTCAAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACTCTAATCTCAAACCGATGCAGGCATTATGGAAAATTGGAAAGGGGGAGTCCCCTCTGATTCCTGATTCTCTTTCAGGAGAGGCACAACATTTCATCCATCAATGCTTAAAAGCTAAGCCAGATGATCGTCCCACTGCTGCTCAGCTTTTAGAACATCCATTTGTAAACAAGCCTCTTTCTTCCATGTCTTTTGGCAGGCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

673

Amino Acids

75.01

Weight (kDa)

6.67

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 35 - 206 1.1e-46 TIR domain
TIR_2 PF13676 38 - 133 1e-15 TIR domain
NB-ARC PF00931 222 - 288 8.5e-07 NB-ARC domain
Pkinase PF00069 408 - 660 1.1e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 411 - 656 1.4e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 387, 546
AarI CACCTGC 1 cut(s) 100
Acc16I TGCGCA 1 cut(s) 1600
Acc36I ACCTGC 1 cut(s) 100
Acc65I GGTACC 1 cut(s) 411
AccB1I GGYRCC 2 cut(s) 411, 1472
AccBSI CCGCTC 1 cut(s) 245
AccI GTMKAC 2 cut(s) 729, 1527
AccII CGCG 1 cut(s) 1025
AccIII TCCGGA 1 cut(s) 632
AclWI GGATC 3 cut(s) 203, 274, 1633
AcoI YGGCCR 1 cut(s) 953
AcsI RAATTY 3 cut(s) 369, 852, 1272
AcuI CTGAAG 3 cut(s) 197, 968, 1574
AcyI GRCGYC 1 cut(s) 744
AfaI GTAC 5 cut(s) 408, 413, 889, 1499, 1780
AfiI CCNNNNNNNGG 2 cut(s) 17, 20
AflIII ACRYGT 2 cut(s) 67, 769
AgsI TTSAA 7 cut(s) 221, 263, 644, 857, 1112, 1355, 1361
AjiI CACGTC 1 cut(s) 772
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 9 cut(s) 158, 437, 456, 1441, 1486, 1636, 1757, 1928, 1960
AluI AGCT 9 cut(s) 158, 437, 456, 1441, 1486, 1636, 1757, 1928, 1960
Alw26I GTCTC 1 cut(s) 1525
AlwI GGATC 3 cut(s) 203, 274, 1633
Aor13HI TCCGGA 1 cut(s) 632
AoxI GGCC 5 cut(s) 35, 872, 953, 1710, 1747
ApeKI GCWGC 4 cut(s) 776, 937, 1242, 1952
ApoI RAATTY 3 cut(s) 369, 852, 1272
Asp700I GAANNNNTTC 2 cut(s) 425, 1180
Asp718I GGTACC 1 cut(s) 411
AspLEI GCGC 2 cut(s) 1027, 1601
AspS9I GGNCC 4 cut(s) 35, 131, 872, 1711
AsuC2I CCSGG 2 cut(s) 34, 942
AsuHPI GGTGA 4 cut(s) 81, 979, 991, 1241
AvaII GGWCC 1 cut(s) 131
AxyI CCTNAGG 1 cut(s) 1716
BanI GGYRCC 2 cut(s) 411, 1472
BanII GRGCYC 1 cut(s) 839
BbsI GAAGAC 2 cut(s) 621, 1080
BbvI GCAGC 4 cut(s) 763, 924, 1254, 1939
BccI CCATC 8 cut(s) 406, 716, 958, 1274, 1509, 1702, 1798, 1920
BceAI ACGGC 2 cut(s) 748, 943
BciT130I CCWGG 1 cut(s) 15
BciVI GTATCC 2 cut(s) 838, 1280
BclI TGATCA 1 cut(s) 1321
BcnI CCSGG 2 cut(s) 34, 942
BcoDI GTCTC 1 cut(s) 1525
BfaI CTAG 1 cut(s) 1533
BfuAI ACCTGC 1 cut(s) 100
BfuI GTATCC 2 cut(s) 838, 1280
BglII AGATCT 2 cut(s) 214, 1539
BlpI GCTNAGC 2 cut(s) 1929, 1956
Bme1390I CCNGG 3 cut(s) 15, 34, 942
Bme18I GGWCC 1 cut(s) 131
BmgBI CACGTC 1 cut(s) 772
BmgT120I GGNCC 4 cut(s) 35, 131, 872, 1711
BmiI GGNNCC 8 cut(s) 132, 396, 413, 873, 975, 1474, 1713, 1809
BmrFI CCNGG 3 cut(s) 15, 34, 942
BmrI ACTGGG 1 cut(s) 1809
BmsI GCATC 3 cut(s) 487, 983, 1821
BmuI ACTGGG 1 cut(s) 1809
BpiI GAAGAC 2 cut(s) 621, 1080
BpmI CTGGAG 1 cut(s) 36
Bpu1102I GCTNAGC 2 cut(s) 1929, 1956
BpuEI CTTGAG 1 cut(s) 139
BpuMI CCSGG 2 cut(s) 34, 942
BsaBI GATNNNNATC 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 744
BsaJI CCNNGG 4 cut(s) 759, 941, 959, 1690
BsaWI WCCGGW 2 cut(s) 632, 976
BsaXI ACNNNNNCTCC 4 cut(s) 9, 39, 1110, 1140
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 20
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 4 cut(s) 516, 1158, 1709, 1804
Bse21I CCTNAGG 1 cut(s) 1716
Bse3DI GCAATG 1 cut(s) 1293
Bse8I GATNNNNATC 1 cut(s) 213
BseAI TCCGGA 1 cut(s) 632
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 4 cut(s) 759, 941, 959, 1690
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 2 cut(s) 17, 20
BseMI GCAATG 1 cut(s) 1293
BseMII CTCAG 5 cut(s) 110, 606, 1370, 1707, 1970
BseNI ACTGG 4 cut(s) 516, 1158, 1709, 1804
BseRI GAGGAG 1 cut(s) 196
BseX3I CGGCCG 1 cut(s) 953
BseXI GCAGC 4 cut(s) 763, 924, 1254, 1939
BseYI CCCAGC 1 cut(s) 1333
BsgI GTGCAG 2 cut(s) 762, 1079
Bsh1236I CGCG 1 cut(s) 1025
Bsh1285I CGRYCG 2 cut(s) 956, 1570
BshFI GGCC 5 cut(s) 37, 874, 955, 1712, 1749
BshNI GGYRCC 2 cut(s) 411, 1472
BsiEI CGRYCG 2 cut(s) 956, 1570
BsiSI CCGG 6 cut(s) 33, 38, 633, 941, 977, 1028
BslFI GGGAC 6 cut(s) 811, 1019, 1031, 1601, 1849, 1929
BslI CCNNNNNNNGG 2 cut(s) 17, 20
BsmAI GTCTC 1 cut(s) 1525
BsmFI GGGAC 6 cut(s) 811, 1019, 1031, 1601, 1849, 1929
BsnI GGCC 5 cut(s) 37, 874, 955, 1712, 1749
Bsp1286I GDGCHC 1 cut(s) 839
Bsp13I TCCGGA 1 cut(s) 632
Bsp143I GATC 7 cut(s) 208, 214, 266, 1321, 1539, 1625, 1939
Bsp1720I GCTNAGC 2 cut(s) 1929, 1956
Bsp19I CCATGG 1 cut(s) 759
BspANI GGCC 5 cut(s) 37, 874, 955, 1712, 1749
BspCNI CTCAG 5 cut(s) 109, 607, 1371, 1708, 1969
BspEI TCCGGA 1 cut(s) 632
BspFNI CGCG 1 cut(s) 1025
BspLI GGNNCC 8 cut(s) 132, 396, 413, 873, 975, 1474, 1713, 1809
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 3 cut(s) 203, 274, 1633
BspQI GCTCTTC 1 cut(s) 1380
BspT107I GGYRCC 2 cut(s) 411, 1472
BsrBI CCGCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 1293
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 4 cut(s) 516, 1158, 1709, 1804
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 4 cut(s) 759, 941, 959, 1690
BssMI GATC 7 cut(s) 208, 214, 266, 1321, 1539, 1625, 1939
BssNAI GTATAC 1 cut(s) 1528
BssNI GRCGYC 1 cut(s) 744
BssT1I CCWWGG 2 cut(s) 759, 1690
Bst1107I GTATAC 1 cut(s) 1528
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 3 cut(s) 411, 718, 1783
Bst6I CTCTTC 5 cut(s) 58, 173, 416, 1029, 1380
BstACI GRCGYC 1 cut(s) 744
BstC8I GCNNGC 6 cut(s) 450, 454, 1617, 1638, 1836, 2012
BstDEI CTNAG 6 cut(s) 96, 615, 1379, 1716, 1929, 1956
BstDSI CCRYGG 2 cut(s) 759, 959
BstFNI CGCG 1 cut(s) 1025
BstHHI GCGC 2 cut(s) 1027, 1601
BstKTI GATC 7 cut(s) 211, 217, 269, 1324, 1542, 1628, 1942
BstMAI GTCTC 1 cut(s) 1525
BstMBI GATC 7 cut(s) 208, 214, 266, 1321, 1539, 1625, 1939
BstMCI CGRYCG 2 cut(s) 956, 1570
BstMWI GCNNNNNNNGC 5 cut(s) 653, 741, 943, 952, 1339
BstNI CCWGG 1 cut(s) 15
BstNSI RCATGY 4 cut(s) 71, 452, 1520, 1619
BstSCI CCNGG 3 cut(s) 13, 32, 940
BstUI CGCG 1 cut(s) 1025
BstV1I GCAGC 4 cut(s) 763, 924, 1254, 1939
BstV2I GAAGAC 2 cut(s) 621, 1080
BstX2I RGATCY 3 cut(s) 214, 1539, 1625
BstYI RGATCY 3 cut(s) 214, 1539, 1625
BstZ17I GTATAC 1 cut(s) 1528
BstZI CGGCCG 1 cut(s) 953
Bsu36I CCTNAGG 1 cut(s) 1716
BsuI GTATCC 2 cut(s) 838, 1280
BsuRI GGCC 5 cut(s) 37, 874, 955, 1712, 1749
BtgI CCRYGG 2 cut(s) 759, 959
BtrI CACGTC 1 cut(s) 772
BtsI GCAGTG 1 cut(s) 1947
BtsIMutI CAGTG 5 cut(s) 80, 104, 612, 714, 1947
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 6 cut(s) 450, 454, 1617, 1638, 1836, 2012
CciNI GCGGCCGC 1 cut(s) 953
CfoI GCGC 2 cut(s) 1027, 1601
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 4 cut(s) 35, 131, 872, 1711
CseI GACGC 1 cut(s) 733
Csp6I GTAC 5 cut(s) 407, 412, 888, 1498, 1779
CviQI GTAC 5 cut(s) 407, 412, 888, 1498, 1779
DdeI CTNAG 6 cut(s) 96, 615, 1379, 1716, 1929, 1956
DpnI GATC 7 cut(s) 210, 216, 268, 1323, 1541, 1627, 1941
DpnII GATC 7 cut(s) 208, 214, 266, 1321, 1539, 1625, 1939
DraI TTTAAA 1 cut(s) 504
EaeI YGGCCR 1 cut(s) 953
EagI CGGCCG 1 cut(s) 953
Eam1104I CTCTTC 5 cut(s) 58, 173, 416, 1029, 1380
EarI CTCTTC 5 cut(s) 58, 173, 416, 1029, 1380
EciI GGCGGA 1 cut(s) 1299
EclXI CGGCCG 1 cut(s) 953
Eco130I CCWWGG 2 cut(s) 759, 1690
Eco24I GRGCYC 1 cut(s) 839
Eco47I GGWCC 1 cut(s) 131
Eco52I CGGCCG 1 cut(s) 953
Eco57I CTGAAG 3 cut(s) 197, 968, 1574
Eco81I CCTNAGG 1 cut(s) 1716
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 2 cut(s) 759, 1690
EcoT38I GRGCYC 1 cut(s) 839
ErhI CCWWGG 2 cut(s) 759, 1690
FalI AAGNNNNNCTT 2 cut(s) 1461, 1493
FaqI GGGAC 6 cut(s) 811, 1019, 1031, 1601, 1849, 1929
FauI CCCGC 3 cut(s) 18, 250, 862
FauNDI CATATG 2 cut(s) 649, 1171
FbaI TGATCA 1 cut(s) 1321
FblI GTMKAC 2 cut(s) 729, 1527
FriOI GRGCYC 1 cut(s) 839
FspBI CTAG 1 cut(s) 1533
FspI TGCGCA 1 cut(s) 1600
GlaI GCGC 2 cut(s) 1026, 1600
GsaI CCCAGC 1 cut(s) 1337
GsuI CTGGAG 1 cut(s) 36
HaeIII GGCC 5 cut(s) 37, 874, 955, 1712, 1749
HapII CCGG 6 cut(s) 33, 38, 633, 941, 977, 1028
HgaI GACGC 1 cut(s) 733
HhaI GCGC 2 cut(s) 1027, 1601
Hin1I GRCGYC 1 cut(s) 744
Hin6I GCGC 2 cut(s) 1025, 1599
HinP1I GCGC 2 cut(s) 1025, 1599
HincII GTYRAC 2 cut(s) 1129, 1797
HindII GTYRAC 2 cut(s) 1129, 1797
HindIII AAGCTT 2 cut(s) 435, 1634
HpaI GTTAAC 1 cut(s) 1797
HpaII CCGG 6 cut(s) 33, 38, 633, 941, 977, 1028
HphI GGTGA 4 cut(s) 81, 979, 991, 1241
Hpy166II GTNNAC 7 cut(s) 568, 621, 730, 1129, 1528, 1797, 1981
Hpy188III TCNNGA 9 cut(s) 118, 134, 212, 633, 1012, 1454, 1565, 1877, 1890
Hpy8I GTNNAC 7 cut(s) 568, 621, 730, 1129, 1528, 1797, 1981
Hpy99I CGWCG 3 cut(s) 749, 776, 1051
HpyCH4III ACNGT 3 cut(s) 411, 718, 1783
HpyCH4IV ACGT 2 cut(s) 771, 1496
HpyF10VI GCNNNNNNNGC 5 cut(s) 653, 741, 943, 952, 1339
HpyF3I CTNAG 6 cut(s) 96, 615, 1379, 1716, 1929, 1956
HpySE526I ACGT 2 cut(s) 771, 1496
Hsp92I GRCGYC 1 cut(s) 744
HspAI GCGC 2 cut(s) 1025, 1599
Kpn2I TCCGGA 1 cut(s) 632
KpnI GGTACC 1 cut(s) 415
Ksp22I TGATCA 1 cut(s) 1321
KspAI GTTAAC 1 cut(s) 1797
Kzo9I GATC 7 cut(s) 208, 214, 266, 1321, 1539, 1625, 1939
LguI GCTCTTC 1 cut(s) 1380
Lsp1109I GCAGC 4 cut(s) 763, 924, 1254, 1939
LweI GCATC 3 cut(s) 487, 983, 1821
MaeI CTAG 1 cut(s) 1533
MaeII ACGT 2 cut(s) 771, 1496
MaeIII GTNAC 3 cut(s) 712, 1210, 1462
MalI GATC 7 cut(s) 210, 216, 268, 1323, 1541, 1627, 1941
MbiI CCGCTC 1 cut(s) 245
MboI GATC 7 cut(s) 208, 214, 266, 1321, 1539, 1625, 1939
MfeI CAATTG 3 cut(s) 258, 279, 573
MflI RGATCY 3 cut(s) 214, 1539, 1625
MhlI GDGCHC 1 cut(s) 839
MlyI GAGTC 5 cut(s) 1052, 1390, 1744, 1777, 1871
MmeI TCCRAC 4 cut(s) 273, 898, 1029, 1767
MroI TCCGGA 1 cut(s) 632
MroXI GAANNNNTTC 2 cut(s) 425, 1180
MseI TTAA 8 cut(s) 192, 441, 503, 924, 1593, 1725, 1796, 1922
MslI CAYNNNNRTG 1 cut(s) 1620
MspA1I CMGCKG 2 cut(s) 1245, 1757
MspI CCGG 6 cut(s) 33, 38, 633, 941, 977, 1028
MspR9I CCNGG 3 cut(s) 15, 34, 942
MunI CAATTG 3 cut(s) 258, 279, 573
MvaI CCWGG 1 cut(s) 15
MvnI CGCG 1 cut(s) 1025
MwoI GCNNNNNNNGC 5 cut(s) 653, 741, 943, 952, 1339
NciI CCSGG 2 cut(s) 34, 942
NcoI CCATGG 1 cut(s) 759
NdeI CATATG 2 cut(s) 649, 1171
NdeII GATC 7 cut(s) 208, 214, 266, 1321, 1539, 1625, 1939
NlaIV GGNNCC 8 cut(s) 132, 396, 413, 873, 975, 1474, 1713, 1809
NmeAIII GCCGAG 2 cut(s) 900, 1218
NmuCI GTSAC 2 cut(s) 712, 1210
NotI GCGGCCGC 1 cut(s) 953
NsbI TGCGCA 1 cut(s) 1600
NspI RCATGY 4 cut(s) 71, 452, 1520, 1619
PaeI GCATGC 2 cut(s) 452, 1619
PaqCI CACCTGC 1 cut(s) 100
PciI ACATGT 1 cut(s) 67
PciSI GCTCTTC 1 cut(s) 1380
PdmI GAANNNNTTC 2 cut(s) 425, 1180
PfeI GAWTC 6 cut(s) 86, 1433, 1511, 1579, 1873, 1880
PflFI GACNNNGTC 1 cut(s) 710
PleI GAGTC 5 cut(s) 1051, 1389, 1743, 1776, 1870
PpsI GAGTC 5 cut(s) 1051, 1389, 1743, 1776, 1870
PscI ACATGT 1 cut(s) 67
PsiI TTATAA 2 cut(s) 387, 546
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 1333
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 8 cut(s) 132, 396, 413, 873, 975, 1474, 1713, 1809
PspPI GGNCC 4 cut(s) 35, 131, 872, 1711
PsrI GAACNNNNNNTAC 2 cut(s) 98, 130
PsuI RGATCY 3 cut(s) 214, 1539, 1625
PsyI GACNNNGTC 1 cut(s) 710
PvuII CAGCTG 1 cut(s) 1757
RsaI GTAC 5 cut(s) 408, 413, 889, 1499, 1780
RsaNI GTAC 5 cut(s) 407, 412, 888, 1498, 1779
RseI CAYNNNNRTG 1 cut(s) 1620
SapI GCTCTTC 1 cut(s) 1380
SaqAI TTAA 8 cut(s) 192, 441, 503, 924, 1593, 1725, 1796, 1922
Sau3AI GATC 7 cut(s) 208, 214, 266, 1321, 1539, 1625, 1939
Sau96I GGNCC 4 cut(s) 35, 131, 872, 1711
SchI GAGTC 5 cut(s) 1052, 1390, 1744, 1777, 1871
ScrFI CCNGG 3 cut(s) 15, 34, 942
SduI GDGCHC 1 cut(s) 839
SfaNI GCATC 3 cut(s) 487, 983, 1821
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 1620
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
SphI GCATGC 2 cut(s) 452, 1619
SspMI CTAG 1 cut(s) 1533
StyD4I CCNGG 3 cut(s) 13, 32, 940
StyI CCWWGG 2 cut(s) 759, 1690
TaaI ACNGT 3 cut(s) 411, 718, 1783
TaiI ACGT 2 cut(s) 774, 1499
TaqI TCGA 3 cut(s) 142, 172, 998
TaqII GACCGA 1 cut(s) 1584
TatI WGTACW 2 cut(s) 887, 1778
TauI GCSGC 6 cut(s) 32, 487, 937, 955, 958, 1248
TfiI GAWTC 6 cut(s) 86, 1433, 1511, 1579, 1873, 1880
Tru1I TTAA 8 cut(s) 192, 441, 503, 924, 1593, 1725, 1796, 1922
Tru9I TTAA 8 cut(s) 192, 441, 503, 924, 1593, 1725, 1796, 1922
TscAI CASTG 5 cut(s) 80, 104, 619, 721, 1954
TseFI GTSAC 2 cut(s) 712, 1210
TseI GCWGC 4 cut(s) 776, 937, 1242, 1952
Tsp45I GTSAC 2 cut(s) 712, 1210
TspRI CASTG 5 cut(s) 80, 104, 619, 721, 1954
Tth111I GACNNNGTC 1 cut(s) 710
VpaK11BI GGWCC 1 cut(s) 131
XapI RAATTY 3 cut(s) 369, 852, 1272
XceI RCATGY 4 cut(s) 71, 452, 1520, 1619
XcmI CCANNNNNNNNNTGG 1 cut(s) 1761
XmiI GTMKAC 2 cut(s) 729, 1527
XmnI GAANNNNTTC 2 cut(s) 425, 1180
XspI CTAG 1 cut(s) 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.