FvH4_5g38970

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000243
Physical Location & Seq
Forward (+)
28785275 .. 28791236
5962 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38970.t1

Sequence Viewer

Length: 1776 bp
ATGCATCATTTACCGCGTTTCTTCTCTAGCAGCAGCAGCGGCAGCGGCAGCAGCAAGGGAGCTAAAGCTAGAGCTAAAGCTGAAGCTAAAGCAAGAGCTAAAGCTGAAGCCAAAGCCATGGATCCGAAGCGGCGGCCCAAATTGGAGCGGCGCAATGCGGTGAAGCACATTGACTACGACGCCTCTACTTCCTCCCCACACTCGCCGCGCCACACGCGCTCGCTCGACTTGACCGACCGGACCAGCTTCCGGGTCGAAGGAAACGGCGAGTTCGAGCGGATTTGCGCCAAGCTAGGGTTTTCCCCGGACGAGTTCGAGATACCCGAGGCGGCCTGGGAGGCCCGGAAGATCCGCTCCACCAACTCGGATGTTCTCCCCATGGCGAAATTCTACGGGATGGATAGTCCGAGACCCGACCCGGGTGACGAATCGGATGACGATAGGGTGGAGGAGCTGTGCGATAGAGTCAGGGATAGCGTTAGTGTTACGGTTGCCGACTCGACTCGGTCCGAATTGGCCGGGCCGAGCGGGTGTTGTAGTTCGAGTAGTAGTAGTTGTAGTGTTAGCGTCGGAATTAAGGGAGTGCGGCCGCCGGGGCTGAAGCCGCCGCCGTCGATGGCTAGGGTTCCGGTGATCGACGATGGATGCTCCACCTGGGATCTTTTGAGGGACTTCGCGCCGGAAGAGGATAGAGAGGCGTCGGACATGGTGCAGAGAAGGTTTCGTCCTCCTTCTTCTTCTTCTTCCGATGAAGAAGAAGAGGAGGAAGAAGAAGAAGAGGAGAATAATGTGGCGGTTGAGATTAGAGAGACGGTGGTGAACTCCGGCGGGTGTTCGTTTACTACTTCCAATGACGATGATTCTTCCAGTAGTACCACAGACCCTTCATACATTTCCCCAAATGGAAGGATTTCGCCGAATGGGAGGCCTAAGCTTGTTATCACTTCTTGGGAGAAGGGTGATCTTCTCGGCAGCGGCTCTTTCGGGTCTGTGTATGAAGGAATTTCTGATGGCGGATGCTTCATTGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAACCCTGGGGAGGCAAAGAGTTTCTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCAGTACCATGGCACACAAAAGGATGAATCAAAGCTTTATATCTTTCTGGAGCTGGTAACCAAAGGCTCCCTTCAAAAGCTGTATCAGACATACCATCTGATAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCATGACCGAAATGTGATTCACAGGGACATTAAATGCGCAAATATTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCAGACTTTGGATTGGCAAAGATTACCAACATGAACGACATAAAATCTTCCAAAGGAACTGCATACTGGATGGCCCCTGAGGTTGTTAATCGTATGAGTCAAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACCGGGCTGGTTCCATACTCGAATCTCGAATGGATGCAGGCATTATGGAAGATTGGAAAGGGGGAGCCCCCTCTGGTTCCTGATTCTCTTTCAAAAGAGGCACAAGATTTCATCCGTCTATGCCTACAAGTCAAGCCAGATAATCGTCCCACTGCGGCTCAGCTTTTAAGACATCCTTTTGTAAATAAGTCCTTTCCTCCCACACCTTCTGGATCAGTATCTCCTTACAATCAGCGCAGACAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

592

Amino Acids

65.24

Weight (kDa)

5.68

Isoelectric Point (pI)

54.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 318 - 570 4e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 320 - 566 8.6e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1330
AccBSI CCGCTC 4 cut(s) 148, 277, 354, 528
AccI GTMKAC 1 cut(s) 1257
AccII CGCG 4 cut(s) 16, 208, 217, 677
AclWI GGATC 6 cut(s) 116, 129, 343, 666, 1363, 1747
AcoI YGGCCR 2 cut(s) 516, 587
AcsI RAATTY 2 cut(s) 386, 1002
AcuI CTGAAG 4 cut(s) 102, 126, 620, 1304
AcyI GRCGYC 2 cut(s) 180, 698
AfaI GTAC 3 cut(s) 874, 1142, 1510
AfiI CCNNNNNNNGG 4 cut(s) 249, 294, 419, 1068
AgsI TTSAA 4 cut(s) 1091, 1121, 1211, 1620
AjnI CCWGG 3 cut(s) 332, 653, 1062
Alw26I GTCTC 3 cut(s) 403, 803, 1255
AlwI GGATC 6 cut(s) 116, 129, 343, 666, 1363, 1747
Ama87I CYCGRG 2 cut(s) 323, 418
AoxI GGCC 9 cut(s) 134, 330, 339, 516, 521, 587, 926, 1440, 1477
ApeKI GCWGC 7 cut(s) 30, 33, 36, 42, 48, 51, 972
ApoI RAATTY 2 cut(s) 386, 1002
Asp700I GAANNNNTTC 1 cut(s) 910
AspLEI GCGC 7 cut(s) 153, 210, 219, 287, 679, 1331, 1764
AspS9I GGNCC 6 cut(s) 135, 240, 340, 507, 521, 1441
AsuC2I CCSGG 8 cut(s) 251, 305, 343, 419, 420, 520, 594, 1531
AsuHPI GGTGA 5 cut(s) 172, 434, 643, 829, 971
AvaI CYCGRG 2 cut(s) 323, 418
AvaII GGWCC 2 cut(s) 240, 507
AxyI CCTNAGG 1 cut(s) 1446
BamHI GGATCC 1 cut(s) 121
BanII GRGCYC 1 cut(s) 1596
BbvI GCAGC 7 cut(s) 42, 45, 48, 54, 60, 63, 984
BccI CCATC 6 cut(s) 391, 610, 635, 1004, 1239, 1432
BceAI ACGGC 2 cut(s) 280, 595
BciT130I CCWGG 3 cut(s) 334, 655, 1064
BclI TGATCA 1 cut(s) 1051
BcnI CCSGG 8 cut(s) 251, 305, 343, 419, 420, 520, 594, 1531
BcoDI GTCTC 3 cut(s) 403, 803, 1255
BfaI CTAG 5 cut(s) 27, 69, 293, 621, 1263
BglI GCCNNNNNGGC 2 cut(s) 338, 595
BglII AGATCT 1 cut(s) 1269
BlpI GCTNAGC 1 cut(s) 1686
Bme18I GGWCC 2 cut(s) 240, 507
BmeT110I CYCGRG 2 cut(s) 323, 418
BmgT120I GGNCC 6 cut(s) 135, 240, 340, 507, 521, 1441
BmiI GGNNCC 8 cut(s) 123, 627, 1060, 1204, 1443, 1539, 1593, 1605
BmsI GCATC 4 cut(s) 13, 635, 1007, 1551
BpmI CTGGAG 1 cut(s) 1205
Bpu10I CCTNAGC 1 cut(s) 930
Bpu1102I GCTNAGC 1 cut(s) 1686
BpuMI CCSGG 8 cut(s) 251, 305, 343, 419, 420, 520, 594, 1531
BsaBI GATNNNNATC 1 cut(s) 1744
BsaHI GRCGYC 2 cut(s) 180, 698
BsaI GGTCTC 1 cut(s) 403
BsaWI WCCGGW 2 cut(s) 237, 628
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 4 cut(s) 249, 294, 419, 1068
Bse1I ACTGG 2 cut(s) 867, 1439
Bse21I CCTNAGG 1 cut(s) 1446
Bse3DI GCAATG 2 cut(s) 160, 1023
Bse8I GATNNNNATC 1 cut(s) 1744
BseBI CCWGG 3 cut(s) 334, 655, 1064
BseJI GATNNNNATC 1 cut(s) 1744
BseLI CCNNNNNNNGG 4 cut(s) 249, 294, 419, 1068
BseMI GCAATG 2 cut(s) 160, 1023
BseMII CTCAG 3 cut(s) 1100, 1437, 1700
BseNI ACTGG 2 cut(s) 867, 1439
BseRI GAGGAG 3 cut(s) 464, 776, 794
BseX3I CGGCCG 1 cut(s) 587
BseXI GCAGC 7 cut(s) 42, 45, 48, 54, 60, 63, 984
BsgI GTGCAG 1 cut(s) 731
Bsh1236I CGCG 4 cut(s) 16, 208, 217, 677
Bsh1285I CGRYCG 2 cut(s) 238, 590
BshFI GGCC 9 cut(s) 136, 332, 341, 518, 523, 589, 928, 1442, 1479
BsiEI CGRYCG 2 cut(s) 238, 590
BsiHKCI CYCGRG 2 cut(s) 323, 418
BslFI GGGAC 3 cut(s) 683, 1331, 1659
BslI CCNNNNNNNGG 4 cut(s) 249, 294, 419, 1068
BsmAI GTCTC 3 cut(s) 403, 803, 1255
BsmBI CGTCTC 1 cut(s) 803
BsmFI GGGAC 3 cut(s) 683, 1331, 1659
BsnI GGCC 9 cut(s) 136, 332, 341, 518, 523, 589, 928, 1442, 1479
Bso31I GGTCTC 1 cut(s) 403
BsoBI CYCGRG 2 cut(s) 323, 418
Bsp1286I GDGCHC 1 cut(s) 1596
Bsp143I GATC 9 cut(s) 121, 348, 633, 658, 961, 1051, 1269, 1355, 1739
Bsp1720I GCTNAGC 1 cut(s) 1686
Bsp19I CCATGG 3 cut(s) 117, 378, 1144
BspANI GGCC 9 cut(s) 136, 332, 341, 518, 523, 589, 928, 1442, 1479
BspCNI CTCAG 3 cut(s) 1101, 1438, 1699
BspFNI CGCG 4 cut(s) 16, 208, 217, 677
BspHI TCATGA 1 cut(s) 1294
BspLI GGNNCC 8 cut(s) 123, 627, 1060, 1204, 1443, 1539, 1593, 1605
BspPI GGATC 6 cut(s) 116, 129, 343, 666, 1363, 1747
BspQI GCTCTTC 1 cut(s) 1110
BspTNI GGTCTC 1 cut(s) 403
BsrBI CCGCTC 4 cut(s) 148, 277, 354, 528
BsrDI GCAATG 2 cut(s) 160, 1023
BsrI ACTGG 2 cut(s) 867, 1439
BssMI GATC 9 cut(s) 121, 348, 633, 658, 961, 1051, 1269, 1355, 1739
BssNAI GTATAC 1 cut(s) 1258
BssNI GRCGYC 2 cut(s) 180, 698
BssT1I CCWWGG 3 cut(s) 117, 378, 1144
Bst1107I GTATAC 1 cut(s) 1258
Bst2UI CCWGG 3 cut(s) 334, 655, 1064
Bst4CI ACNGT 3 cut(s) 490, 814, 1513
Bst6I CTCTTC 4 cut(s) 678, 753, 771, 1110
BstACI GRCGYC 2 cut(s) 180, 698
BstC8I GCNNGC 4 cut(s) 221, 1347, 1368, 1566
BstDEI CTNAG 4 cut(s) 930, 1109, 1446, 1686
BstDSI CCRYGG 3 cut(s) 117, 378, 1144
BstEII GGTNACC 1 cut(s) 1192
BstFNI CGCG 4 cut(s) 16, 208, 217, 677
BstHHI GCGC 7 cut(s) 153, 210, 219, 287, 679, 1331, 1764
BstKTI GATC 9 cut(s) 124, 351, 636, 661, 964, 1054, 1272, 1358, 1742
BstMAI GTCTC 3 cut(s) 403, 803, 1255
BstMBI GATC 9 cut(s) 121, 348, 633, 658, 961, 1051, 1269, 1355, 1739
BstMCI CGRYCG 2 cut(s) 238, 590
BstNI CCWGG 3 cut(s) 334, 655, 1064
BstNSI RCATGY 2 cut(s) 1250, 1349
BstPI GGTNACC 1 cut(s) 1192
BstUI CGCG 4 cut(s) 16, 208, 217, 677
BstV1I GCAGC 7 cut(s) 42, 45, 48, 54, 60, 63, 984
BstX2I RGATCY 5 cut(s) 121, 348, 658, 1269, 1355
BstXI CCANNNNNNTGG 1 cut(s) 118
BstYI RGATCY 5 cut(s) 121, 348, 658, 1269, 1355
BstZ17I GTATAC 1 cut(s) 1258
BstZI CGGCCG 1 cut(s) 587
Bsu36I CCTNAGG 1 cut(s) 1446
BsuRI GGCC 9 cut(s) 136, 332, 341, 518, 523, 589, 928, 1442, 1479
BtgI CCRYGG 3 cut(s) 117, 378, 1144
BtsI GCAGTG 1 cut(s) 1677
BtsIMutI CAGTG 1 cut(s) 1677
Cac8I GCNNGC 4 cut(s) 221, 1347, 1368, 1566
CciI TCATGA 1 cut(s) 1294
CciNI GCGGCCGC 1 cut(s) 587
CfoI GCGC 7 cut(s) 153, 210, 219, 287, 679, 1331, 1764
Cfr13I GGNCC 6 cut(s) 135, 240, 340, 507, 521, 1441
Cfr9I CCCGGG 1 cut(s) 418
CpoI CGGWCCG 1 cut(s) 507
CseI GACGC 3 cut(s) 188, 556, 687
Csp6I GTAC 3 cut(s) 873, 1141, 1509
CspI CGGWCCG 1 cut(s) 507
CviAII CATG 9 cut(s) 118, 379, 706, 1124, 1145, 1247, 1295, 1346, 1399
CviQI GTAC 3 cut(s) 873, 1141, 1509
DdeI CTNAG 4 cut(s) 930, 1109, 1446, 1686
DpnI GATC 9 cut(s) 123, 350, 635, 660, 963, 1053, 1271, 1357, 1741
DpnII GATC 9 cut(s) 121, 348, 633, 658, 961, 1051, 1269, 1355, 1739
EaeI YGGCCR 2 cut(s) 516, 587
EagI CGGCCG 1 cut(s) 587
Eam1104I CTCTTC 4 cut(s) 678, 753, 771, 1110
EarI CTCTTC 4 cut(s) 678, 753, 771, 1110
EciI GGCGGA 1 cut(s) 1029
EclXI CGGCCG 1 cut(s) 587
Eco130I CCWWGG 3 cut(s) 117, 378, 1144
Eco147I AGGCCT 1 cut(s) 928
Eco24I GRGCYC 1 cut(s) 1596
Eco31I GGTCTC 1 cut(s) 403
Eco47I GGWCC 2 cut(s) 240, 507
Eco52I CGGCCG 1 cut(s) 587
Eco57I CTGAAG 4 cut(s) 102, 126, 620, 1304
Eco81I CCTNAGG 1 cut(s) 1446
Eco88I CYCGRG 2 cut(s) 323, 418
Eco91I GGTNACC 1 cut(s) 1192
EcoO65I GGTNACC 1 cut(s) 1192
EcoRII CCWGG 3 cut(s) 332, 653, 1062
EcoT14I CCWWGG 3 cut(s) 117, 378, 1144
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1596
ErhI CCWWGG 3 cut(s) 117, 378, 1144
Esp3I CGTCTC 1 cut(s) 803
FaeI CATG 9 cut(s) 121, 382, 709, 1127, 1148, 1250, 1298, 1349, 1402
FalI AAGNNNNNCTT 4 cut(s) 1191, 1223, 1687, 1719
FaqI GGGAC 3 cut(s) 683, 1331, 1659
FatI CATG 9 cut(s) 117, 378, 705, 1123, 1144, 1246, 1294, 1345, 1398
FauI CCCGC 2 cut(s) 521, 821
FbaI TGATCA 1 cut(s) 1051
FblI GTMKAC 1 cut(s) 1257
FriOI GRGCYC 1 cut(s) 1596
FspBI CTAG 5 cut(s) 27, 69, 293, 621, 1263
FspI TGCGCA 1 cut(s) 1330
GlaI GCGC 7 cut(s) 152, 209, 218, 286, 678, 1330, 1763
GsuI CTGGAG 1 cut(s) 1205
HaeIII GGCC 9 cut(s) 136, 332, 341, 518, 523, 589, 928, 1442, 1479
HgaI GACGC 3 cut(s) 188, 556, 687
HhaI GCGC 7 cut(s) 153, 210, 219, 287, 679, 1331, 1764
Hin1I GRCGYC 2 cut(s) 180, 698
Hin1II CATG 9 cut(s) 121, 382, 709, 1127, 1148, 1250, 1298, 1349, 1402
Hin6I GCGC 7 cut(s) 151, 208, 217, 285, 677, 1329, 1762
HinP1I GCGC 7 cut(s) 151, 208, 217, 285, 677, 1329, 1762
HincII GTYRAC 1 cut(s) 1527
HindII GTYRAC 1 cut(s) 1527
HindIII AAGCTT 3 cut(s) 932, 1169, 1364
HpaI GTTAAC 1 cut(s) 1527
HphI GGTGA 5 cut(s) 172, 434, 643, 829, 971
Hpy166II GTNNAC 4 cut(s) 820, 840, 1258, 1527
Hpy188III TCNNGA 6 cut(s) 316, 1184, 1295, 1553, 1607, 1737
Hpy8I GTNNAC 4 cut(s) 820, 840, 1258, 1527
Hpy99I CGWCG 5 cut(s) 182, 572, 616, 641, 703
HpyCH4III ACNGT 3 cut(s) 490, 814, 1513
HpyCH4V TGCA 7 cut(s) 4, 712, 1276, 1345, 1370, 1430, 1564
HpyF3I CTNAG 4 cut(s) 930, 1109, 1446, 1686
Hsp92I GRCGYC 2 cut(s) 180, 698
Hsp92II CATG 9 cut(s) 121, 382, 709, 1127, 1148, 1250, 1298, 1349, 1402
HspAI GCGC 7 cut(s) 151, 208, 217, 285, 677, 1329, 1762
Ksp22I TGATCA 1 cut(s) 1051
KspAI GTTAAC 1 cut(s) 1527
Kzo9I GATC 9 cut(s) 121, 348, 633, 658, 961, 1051, 1269, 1355, 1739
LguI GCTCTTC 1 cut(s) 1110
LmnI GCTCC 8 cut(s) 59, 145, 359, 451, 653, 1186, 1208, 1591
Lsp1109I GCAGC 7 cut(s) 42, 45, 48, 54, 60, 63, 984
LweI GCATC 4 cut(s) 13, 635, 1007, 1551
MaeI CTAG 5 cut(s) 27, 69, 293, 621, 1263
MaeIII GTNAC 3 cut(s) 422, 484, 1192
MalI GATC 9 cut(s) 123, 350, 635, 660, 963, 1053, 1271, 1357, 1741
MbiI CCGCTC 4 cut(s) 148, 277, 354, 528
MboI GATC 9 cut(s) 121, 348, 633, 658, 961, 1051, 1269, 1355, 1739
MflI RGATCY 5 cut(s) 121, 348, 658, 1269, 1355
MhlI GDGCHC 1 cut(s) 1596
MluCI AATT 5 cut(s) 140, 386, 512, 573, 1002
MlyI GAGTC 6 cut(s) 474, 491, 496, 1120, 1474, 1507
MmeI TCCRAC 3 cut(s) 550, 681, 1497
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 910
MseI TTAA 6 cut(s) 576, 1323, 1455, 1526, 1694, 1774
MslI CAYNNNNRTG 1 cut(s) 1350
MspA1I CMGCKG 4 cut(s) 39, 45, 975, 1487
MvaI CCWGG 3 cut(s) 334, 655, 1064
MvnI CGCG 4 cut(s) 16, 208, 217, 677
NciI CCSGG 8 cut(s) 251, 305, 343, 419, 420, 520, 594, 1531
NcoI CCATGG 3 cut(s) 117, 378, 1144
NdeII GATC 9 cut(s) 121, 348, 633, 658, 961, 1051, 1269, 1355, 1739
NlaIII CATG 9 cut(s) 121, 382, 709, 1127, 1148, 1250, 1298, 1349, 1402
NlaIV GGNNCC 8 cut(s) 123, 627, 1060, 1204, 1443, 1539, 1593, 1605
NmeAIII GCCGAG 2 cut(s) 549, 948
NmuCI GTSAC 1 cut(s) 422
NotI GCGGCCGC 1 cut(s) 587
NsbI TGCGCA 1 cut(s) 1330
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 1250, 1349
PaeI GCATGC 1 cut(s) 1349
PagI TCATGA 1 cut(s) 1294
PasI CCCWGGG 1 cut(s) 1063
PceI AGGCCT 1 cut(s) 928
PciSI GCTCTTC 1 cut(s) 1110
PcsI WCGNNNNNNNCGW 3 cut(s) 231, 270, 321
PdmI GAANNNNTTC 1 cut(s) 910
PfeI GAWTC 7 cut(s) 428, 860, 1163, 1241, 1309, 1549, 1610
PflFI GACNNNGTC 1 cut(s) 505
PleI GAGTC 6 cut(s) 473, 491, 496, 1119, 1473, 1506
PpsI GAGTC 6 cut(s) 473, 491, 496, 1119, 1473, 1506
Psp6I CCWGG 3 cut(s) 332, 653, 1062
PspEI GGTNACC 1 cut(s) 1192
PspGI CCWGG 3 cut(s) 332, 653, 1062
PspN4I GGNNCC 8 cut(s) 123, 627, 1060, 1204, 1443, 1539, 1593, 1605
PspPI GGNCC 6 cut(s) 135, 240, 340, 507, 521, 1441
PsuI RGATCY 5 cut(s) 121, 348, 658, 1269, 1355
PsyI GACNNNGTC 1 cut(s) 505
PvuII CAGCTG 1 cut(s) 1487
RsaI GTAC 3 cut(s) 874, 1142, 1510
RsaNI GTAC 3 cut(s) 873, 1141, 1509
RseI CAYNNNNRTG 1 cut(s) 1350
Rsr2I CGGWCCG 1 cut(s) 507
RsrII CGGWCCG 1 cut(s) 507
SapI GCTCTTC 1 cut(s) 1110
SaqAI TTAA 6 cut(s) 576, 1323, 1455, 1526, 1694, 1774
Sau3AI GATC 9 cut(s) 121, 348, 633, 658, 961, 1051, 1269, 1355, 1739
Sau96I GGNCC 6 cut(s) 135, 240, 340, 507, 521, 1441
SchI GAGTC 6 cut(s) 474, 491, 496, 1120, 1474, 1507
SduI GDGCHC 1 cut(s) 1596
SfaNI GCATC 4 cut(s) 13, 635, 1007, 1551
SfiI GGCCNNNNNGGCC 1 cut(s) 338
SinI GGWCC 2 cut(s) 240, 507
SmaI CCCGGG 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 1350
SphI GCATGC 1 cut(s) 1349
Sse9I AATT 5 cut(s) 140, 386, 512, 573, 1002
SseBI AGGCCT 1 cut(s) 928
SspI AATATT 1 cut(s) 1336
SspMI CTAG 5 cut(s) 27, 69, 293, 621, 1263
StuI AGGCCT 1 cut(s) 928
StyI CCWWGG 3 cut(s) 117, 378, 1144
TaaI ACNGT 3 cut(s) 490, 814, 1513
TaqII GACCGA 3 cut(s) 248, 495, 1314
TasI AATT 5 cut(s) 140, 386, 512, 573, 1002
TatI WGTACW 1 cut(s) 1508
TfiI GAWTC 7 cut(s) 428, 860, 1163, 1241, 1309, 1549, 1610
Tru1I TTAA 6 cut(s) 576, 1323, 1455, 1526, 1694, 1774
Tru9I TTAA 6 cut(s) 576, 1323, 1455, 1526, 1694, 1774
TscAI CASTG 1 cut(s) 1684
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 7 cut(s) 30, 33, 36, 42, 48, 51, 972
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 8 cut(s) 765, 876, 1011, 1012, 1176, 1283, 1415, 1627
TspGWI ACGGA 1 cut(s) 1631
TspMI CCCGGG 1 cut(s) 418
TspRI CASTG 1 cut(s) 1684
Tth111I GACNNNGTC 1 cut(s) 505
VpaK11BI GGWCC 2 cut(s) 240, 507
XapI RAATTY 2 cut(s) 386, 1002
XceI RCATGY 2 cut(s) 1250, 1349
XcmI CCANNNNNNNNNTGG 1 cut(s) 1491
XmaI CCCGGG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 1257
XmnI GAANNNNTTC 1 cut(s) 910
XspI CTAG 5 cut(s) 27, 69, 293, 621, 1263
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.