Rh7CG513000

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000243
Physical Location & Seq
Forward (+)
69215431 .. 69221624
6194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG513000.1

Sequence Viewer

Length: 1467 bp
ATGCATCACTTACCGCGTCTTTTCTCCAGCAAGAAGGATCGAACTAAATCCATGGATCCGAAGAAGGCCCGGCGGTTGCAGCGCCGCAATGCGGTCAAGCACATTGACTACGACGCCTCTTCGTCCCCCTCCTCCCGCGACAACTCCACCCACGCGCAGCAGCTCCACACGCGCTCGCTCGACCTGTCGGACCGGACCAGCTTCCGGGTCGAAGGAAATGACGGCGAGTTCGAACGGATTTGCGCCGAGCTGGGATTCTCCGGCCCAGACGACTTCGCGATACCCGAGGCGGCCTGGGAGTCCCGGAGGATCCGGTCCACCAACTCGGATGTTCTCCCCCTGTCAAAAGTGTACCAGATGGATAGTCCGAGGCCCGACCCGAAGGACGAATCGGAGGAGAATGATGCCGTGGCGGAATTGAGCAATAGAGTTAGGGATAGCGTTACTGTTACGGTGGCCGAGTCGACTCGAGCCGAGTCTGAGTCGGCGGGGCCGAGCGGGTGTTGTACTGCGAGTAGTAGTTGTAGTAGTGTTAGTGTCGGAATTAAGGGAGCGCGGCCGCCGGGGCTGAGGCCGCCGCCGTCGATGACTAGGGTTCCGGTGATTGACAATGGATGCTCGACTTGGGACATTTTGAGGGACTTCGCTCCTGAAGAAGAGAGGGAAATGGTGCGGAGAAGGTTTGTTCCTTCTTCTTCTGATGATGATGAAGAAGAAGAAGAAGAAGAACTTGAAGAAGAACAAGAACTAGAAGAAGAACTTGAAGAAGGAGAGGATGTTGTGGCGGTTGAGATTAGAGAGACTGTGGCAAACTCCGGCGGGTGTTCGTTTACTACTTCCAATGATGATGACTCATCCAGCACCACCACAGAGCCTTCAAATATTTCTCCAAATGGAAGGTTTTCGCCGAATGGGAAGCCTAAGATTATTGTCACTAGTTGGGAGAAGGGTGATCTTCTCGGCAGCGGCTCTTTCGGGTCTGTGTATGAAGGAATTTCTGATGGCGGATGCTTCATTGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGGAGGCAAAGAGTTTCTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGAATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAGACGTACCATCTTACAGATTCGCATGTCTCTGTATACACAAGACAGATCTTGCAAGGTCTGATGTATCTTCACGACCGAAGAGTGATTCACAGGGACATTAAATGTGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCAGACTTTGGATTGGCAAAGACTATCCAAATGAACGACATAAAATCTTGCCAAGGAACTGCATACTGGATGGCACCTGAGGTTTGCTTTTGCACTTTTGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

488

Amino Acids

54.38

Weight (kDa)

4.87

Isoelectric Point (pI)

58.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 315 - 481 2.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 317 - 482 1.8e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1432
AccBSI CCGCTC 1 cut(s) 498
AccI GTMKAC 2 cut(s) 464, 1248
AccII CGCG 6 cut(s) 16, 138, 155, 172, 278, 556
AclWI GGATC 6 cut(s) 45, 50, 63, 304, 317, 1354
AcoI YGGCCR 2 cut(s) 456, 557
AcsI RAATTY 2 cut(s) 993, 1460
AcuI CTGAAG 1 cut(s) 672
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 3 cut(s) 353, 508, 1220
AfiI CCNNNNNNNGG 2 cut(s) 91, 204
AgsI TTSAA 7 cut(s) 734, 764, 879, 1082, 1112, 1202, 1460
AhlI ACTAGT 1 cut(s) 935
AjnI CCWGG 2 cut(s) 293, 1053
AluBI AGCT 6 cut(s) 163, 201, 250, 1162, 1207, 1357
AluI AGCT 6 cut(s) 163, 201, 250, 1162, 1207, 1357
Alw26I GTCTC 2 cut(s) 794, 1246
AlwI GGATC 6 cut(s) 45, 50, 63, 304, 317, 1354
Ama87I CYCGRG 2 cut(s) 284, 468
AoxI GGCC 8 cut(s) 66, 262, 291, 371, 456, 491, 557, 572
ApeKI GCWGC 4 cut(s) 79, 157, 160, 963
ApoI RAATTY 2 cut(s) 993, 1460
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
Asp700I GAANNNNTTC 1 cut(s) 901
AspLEI GCGC 5 cut(s) 84, 157, 174, 245, 556
AspS9I GGNCC 7 cut(s) 67, 190, 195, 263, 315, 372, 491
AsuC2I CCSGG 4 cut(s) 70, 206, 304, 564
AsuHPI GGTGA 2 cut(s) 613, 962
AsuII TTCGAA 1 cut(s) 231
AvaI CYCGRG 2 cut(s) 284, 468
AvaII GGWCC 3 cut(s) 190, 195, 315
AxyI CCTNAGG 1 cut(s) 1437
BamHI GGATCC 2 cut(s) 55, 309
BanI GGYRCC 1 cut(s) 1432
BbvCI CCTCAGC 1 cut(s) 569
BbvI GCAGC 4 cut(s) 91, 169, 172, 975
BccI CCATC 4 cut(s) 352, 995, 1230, 1423
BceAI ACGGC 3 cut(s) 238, 392, 565
BciT130I CCWGG 2 cut(s) 295, 1055
BclI TGATCA 1 cut(s) 1042
BcnI CCSGG 4 cut(s) 70, 206, 304, 564
BcoDI GTCTC 2 cut(s) 794, 1246
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 3 cut(s) 591, 749, 936
BfoI RGCGCY 1 cut(s) 85
BglI GCCNNNNNGGC 1 cut(s) 565
BglII AGATCT 1 cut(s) 1260
Bme1390I CCNGG 6 cut(s) 70, 206, 295, 304, 564, 1055
Bme18I GGWCC 3 cut(s) 190, 195, 315
BmeT110I CYCGRG 2 cut(s) 284, 468
BmgT120I GGNCC 7 cut(s) 67, 190, 195, 263, 315, 372, 491
BmiI GGNNCC 6 cut(s) 57, 311, 492, 597, 1195, 1434
BmrFI CCNGG 6 cut(s) 70, 206, 295, 304, 564, 1055
BmsI GCATC 4 cut(s) 13, 394, 605, 998
BpmI CTGGAG 1 cut(s) 10
Bpu10I CCTNAGC 1 cut(s) 569
Bpu14I TTCGAA 1 cut(s) 231
BpuMI CCSGG 4 cut(s) 70, 206, 304, 564
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 8 cut(s) 51, 285, 294, 368, 408, 563, 1054, 1411
BsaWI WCCGGW 3 cut(s) 192, 312, 598
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 204
Bse1I ACTGG 1 cut(s) 1430
Bse21I CCTNAGG 1 cut(s) 1437
Bse3DI GCAATG 2 cut(s) 94, 1014
BseBI CCWGG 2 cut(s) 295, 1055
BseDI CCNNGG 8 cut(s) 51, 285, 294, 368, 408, 563, 1054, 1411
BseGI GGATG 7 cut(s) 334, 620, 781, 854, 1013, 1156, 1434
BseLI CCNNNNNNNGG 2 cut(s) 91, 204
BseMI GCAATG 2 cut(s) 94, 1014
BseMII CTCAG 4 cut(s) 471, 560, 1091, 1428
BseNI ACTGG 1 cut(s) 1430
BseRI GAGGAG 2 cut(s) 121, 410
BseX3I CGGCCG 1 cut(s) 557
BseXI GCAGC 4 cut(s) 91, 169, 172, 975
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 6 cut(s) 16, 138, 155, 172, 278, 556
Bsh1285I CGRYCG 2 cut(s) 560, 1291
BshFI GGCC 8 cut(s) 68, 264, 293, 373, 458, 493, 559, 574
BshNI GGYRCC 1 cut(s) 1432
BsiEI CGRYCG 2 cut(s) 560, 1291
BsiHKCI CYCGRG 2 cut(s) 284, 468
BsiSI CCGG 9 cut(s) 70, 193, 205, 261, 304, 313, 563, 599, 816
BslFI GGGAC 5 cut(s) 109, 286, 641, 653, 1322
BslI CCNNNNNNNGG 2 cut(s) 91, 204
BsmAI GTCTC 2 cut(s) 794, 1246
BsmFI GGGAC 5 cut(s) 109, 286, 641, 653, 1322
BsnI GGCC 8 cut(s) 68, 264, 293, 373, 458, 493, 559, 574
BsoBI CYCGRG 2 cut(s) 284, 468
Bsp119I TTCGAA 1 cut(s) 231
Bsp143I GATC 7 cut(s) 37, 55, 309, 952, 1042, 1260, 1346
Bsp19I CCATGG 1 cut(s) 51
Bsp68I TCGCGA 1 cut(s) 278
BspANI GGCC 8 cut(s) 68, 264, 293, 373, 458, 493, 559, 574
BspCNI CTCAG 4 cut(s) 472, 561, 1092, 1429
BspFNI CGCG 6 cut(s) 16, 138, 155, 172, 278, 556
BspLI GGNNCC 6 cut(s) 57, 311, 492, 597, 1195, 1434
BspPI GGATC 6 cut(s) 45, 50, 63, 304, 317, 1354
BspQI GCTCTTC 1 cut(s) 1101
BspT104I TTCGAA 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 1432
BsrBI CCGCTC 1 cut(s) 498
BsrDI GCAATG 2 cut(s) 94, 1014
BsrI ACTGG 1 cut(s) 1430
BssECI CCNNGG 8 cut(s) 51, 285, 294, 368, 408, 563, 1054, 1411
BssMI GATC 7 cut(s) 37, 55, 309, 952, 1042, 1260, 1346
BssNAI GTATAC 1 cut(s) 1249
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 51, 1411
Bst1107I GTATAC 1 cut(s) 1249
Bst2UI CCWGG 2 cut(s) 295, 1055
Bst4CI ACNGT 3 cut(s) 448, 454, 805
Bst6I CTCTTC 4 cut(s) 124, 651, 1101, 1288
BstACI GRCGYC 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 3 cut(s) 176, 1338, 1359
BstDEI CTNAG 5 cut(s) 480, 569, 921, 1100, 1437
BstDSI CCRYGG 2 cut(s) 51, 408
BstF5I GGATG 7 cut(s) 334, 620, 781, 854, 1013, 1156, 1434
BstFNI CGCG 6 cut(s) 16, 138, 155, 172, 278, 556
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 5 cut(s) 84, 157, 174, 245, 556
BstKTI GATC 7 cut(s) 40, 58, 312, 955, 1045, 1263, 1349
BstMAI GTCTC 2 cut(s) 794, 1246
BstMBI GATC 7 cut(s) 37, 55, 309, 952, 1042, 1260, 1346
BstMCI CGRYCG 2 cut(s) 560, 1291
BstMWI GCNNNNNNNGC 4 cut(s) 79, 169, 565, 574
BstNI CCWGG 2 cut(s) 295, 1055
BstNSI RCATGY 2 cut(s) 1241, 1340
BstSCI CCNGG 6 cut(s) 68, 204, 293, 302, 562, 1053
BstUI CGCG 6 cut(s) 16, 138, 155, 172, 278, 556
BstV1I GCAGC 4 cut(s) 91, 169, 172, 975
BstX2I RGATCY 4 cut(s) 55, 309, 1260, 1346
BstYI RGATCY 4 cut(s) 55, 309, 1260, 1346
BstZ17I GTATAC 1 cut(s) 1249
BstZI CGGCCG 1 cut(s) 557
Bsu36I CCTNAGG 1 cut(s) 1437
BsuRI GGCC 8 cut(s) 68, 264, 293, 373, 458, 493, 559, 574
BtgI CCRYGG 2 cut(s) 51, 408
BtsCI GGATG 7 cut(s) 334, 620, 781, 854, 1013, 1156, 1434
BtuMI TCGCGA 1 cut(s) 278
Cac8I GCNNGC 3 cut(s) 176, 1338, 1359
CciNI GCGGCCGC 1 cut(s) 557
CfoI GCGC 5 cut(s) 84, 157, 174, 245, 556
Cfr13I GGNCC 7 cut(s) 67, 190, 195, 263, 315, 372, 491
CpoI CGGWCCG 1 cut(s) 190
CseI GACGC 2 cut(s) 5, 122
Csp6I GTAC 3 cut(s) 352, 507, 1219
CspI CGGWCCG 1 cut(s) 190
CviAII CATG 4 cut(s) 52, 1115, 1238, 1337
CviQI GTAC 3 cut(s) 352, 507, 1219
DdeI CTNAG 5 cut(s) 480, 569, 921, 1100, 1437
DpnI GATC 7 cut(s) 39, 57, 311, 954, 1044, 1262, 1348
DpnII GATC 7 cut(s) 37, 55, 309, 952, 1042, 1260, 1346
EaeI YGGCCR 2 cut(s) 456, 557
EagI CGGCCG 1 cut(s) 557
Eam1104I CTCTTC 4 cut(s) 124, 651, 1101, 1288
EarI CTCTTC 4 cut(s) 124, 651, 1101, 1288
EciI GGCGGA 2 cut(s) 428, 1020
EclXI CGGCCG 1 cut(s) 557
Eco130I CCWWGG 2 cut(s) 51, 1411
Eco47I GGWCC 3 cut(s) 190, 195, 315
Eco52I CGGCCG 1 cut(s) 557
Eco57I CTGAAG 1 cut(s) 672
Eco81I CCTNAGG 1 cut(s) 1437
Eco88I CYCGRG 2 cut(s) 284, 468
EcoRII CCWGG 2 cut(s) 293, 1053
EcoT14I CCWWGG 2 cut(s) 51, 1411
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 51, 1411
FaeI CATG 4 cut(s) 55, 1118, 1241, 1340
FalI AAGNNNNNCTT 6 cut(s) 714, 746, 744, 776, 1182, 1214
FaqI GGGAC 5 cut(s) 109, 286, 641, 653, 1322
FatI CATG 4 cut(s) 51, 1114, 1237, 1336
FauI CCCGC 4 cut(s) 143, 481, 491, 812
FbaI TGATCA 1 cut(s) 1042
FblI GTMKAC 2 cut(s) 464, 1248
FokI GGATG 7 cut(s) 341, 627, 788, 841, 1020, 1163, 1441
FspBI CTAG 3 cut(s) 591, 749, 936
GlaI GCGC 5 cut(s) 83, 156, 173, 244, 555
GsaI CCCAGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 10
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 8 cut(s) 68, 264, 293, 373, 458, 493, 559, 574
HapII CCGG 9 cut(s) 70, 193, 205, 261, 304, 313, 563, 599, 816
HgaI GACGC 2 cut(s) 5, 122
HhaI GCGC 5 cut(s) 84, 157, 174, 245, 556
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 4 cut(s) 55, 1118, 1241, 1340
Hin6I GCGC 5 cut(s) 82, 155, 172, 243, 554
HinP1I GCGC 5 cut(s) 82, 155, 172, 243, 554
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HindIII AAGCTT 1 cut(s) 1355
HpaII CCGG 9 cut(s) 70, 193, 205, 261, 304, 313, 563, 599, 816
HphI GGTGA 2 cut(s) 613, 962
Hpy166II GTNNAC 5 cut(s) 318, 352, 465, 831, 1249
Hpy188III TCNNGA 4 cut(s) 277, 650, 1175, 1286
Hpy8I GTNNAC 5 cut(s) 318, 352, 465, 831, 1249
Hpy99I CGWCG 2 cut(s) 116, 586
HpyCH4III ACNGT 3 cut(s) 448, 454, 805
HpyCH4IV ACGT 1 cut(s) 1217
HpyCH4V TGCA 8 cut(s) 4, 79, 1267, 1322, 1336, 1361, 1421, 1452
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 169, 565, 574
HpyF3I CTNAG 5 cut(s) 480, 569, 921, 1100, 1437
HpySE526I ACGT 1 cut(s) 1217
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 4 cut(s) 55, 1118, 1241, 1340
HspAI GCGC 5 cut(s) 82, 155, 172, 243, 554
Ksp22I TGATCA 1 cut(s) 1042
Kzo9I GATC 7 cut(s) 37, 55, 309, 952, 1042, 1260, 1346
LguI GCTCTTC 1 cut(s) 1101
LmnI GCTCC 4 cut(s) 168, 551, 652, 1199
Lsp1109I GCAGC 4 cut(s) 91, 169, 172, 975
LweI GCATC 4 cut(s) 13, 394, 605, 998
MaeI CTAG 3 cut(s) 591, 749, 936
MaeII ACGT 1 cut(s) 1217
MaeIII GTNAC 4 cut(s) 442, 448, 931, 1183
MalI GATC 7 cut(s) 39, 57, 311, 954, 1044, 1262, 1348
MbiI CCGCTC 1 cut(s) 498
MboI GATC 7 cut(s) 37, 55, 309, 952, 1042, 1260, 1346
MflI RGATCY 4 cut(s) 55, 309, 1260, 1346
MluCI AATT 4 cut(s) 416, 543, 993, 1460
MlyI GAGTC 7 cut(s) 308, 460, 470, 485, 491, 845, 1111
MmeI TCCRAC 2 cut(s) 168, 520
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 901
MseI TTAA 3 cut(s) 546, 1314, 1465
MslI CAYNNNNRTG 1 cut(s) 1341
MspA1I CMGCKG 1 cut(s) 966
MspI CCGG 9 cut(s) 70, 193, 205, 261, 304, 313, 563, 599, 816
MspR9I CCNGG 6 cut(s) 70, 206, 295, 304, 564, 1055
MvaI CCWGG 2 cut(s) 295, 1055
MvnI CGCG 6 cut(s) 16, 138, 155, 172, 278, 556
MwoI GCNNNNNNNGC 4 cut(s) 79, 169, 565, 574
NciI CCSGG 4 cut(s) 70, 206, 304, 564
NcoI CCATGG 1 cut(s) 51
NdeII GATC 7 cut(s) 37, 55, 309, 952, 1042, 1260, 1346
NlaIII CATG 4 cut(s) 55, 1118, 1241, 1340
NlaIV GGNNCC 6 cut(s) 57, 311, 492, 597, 1195, 1434
NmeAIII GCCGAG 5 cut(s) 271, 484, 499, 519, 939
NmuCI GTSAC 1 cut(s) 931
NotI GCGGCCGC 1 cut(s) 557
NruI TCGCGA 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 1241, 1340
NspV TTCGAA 1 cut(s) 231
PaeI GCATGC 1 cut(s) 1340
PaeR7I CTCGAG 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 1101
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 282, 491
PdmI GAANNNNTTC 1 cut(s) 901
PfeI GAWTC 5 cut(s) 255, 389, 1154, 1232, 1300
PfoI TCCNGGA 1 cut(s) 302
PleI GAGTC 7 cut(s) 307, 460, 469, 484, 490, 845, 1110
PpsI GAGTC 7 cut(s) 307, 460, 469, 484, 490, 845, 1110
Psp6I CCWGG 2 cut(s) 293, 1053
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 2 cut(s) 293, 1053
PspN4I GGNNCC 6 cut(s) 57, 311, 492, 597, 1195, 1434
PspPI GGNCC 7 cut(s) 67, 190, 195, 263, 315, 372, 491
PspXI VCTCGAGB 1 cut(s) 468
PsuI RGATCY 4 cut(s) 55, 309, 1260, 1346
RruI TCGCGA 1 cut(s) 278
RsaI GTAC 3 cut(s) 353, 508, 1220
RsaNI GTAC 3 cut(s) 352, 507, 1219
RseI CAYNNNNRTG 1 cut(s) 1341
Rsr2I CGGWCCG 1 cut(s) 190
RsrII CGGWCCG 1 cut(s) 190
SalI GTCGAC 1 cut(s) 463
SapI GCTCTTC 1 cut(s) 1101
SaqAI TTAA 3 cut(s) 546, 1314, 1465
Sau3AI GATC 7 cut(s) 37, 55, 309, 952, 1042, 1260, 1346
Sau96I GGNCC 7 cut(s) 67, 190, 195, 263, 315, 372, 491
SchI GAGTC 7 cut(s) 308, 460, 470, 485, 491, 845, 1111
ScrFI CCNGG 6 cut(s) 70, 206, 295, 304, 564, 1055
SfaNI GCATC 4 cut(s) 13, 394, 605, 998
Sfr274I CTCGAG 1 cut(s) 468
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 3 cut(s) 190, 195, 315
SlaI CTCGAG 1 cut(s) 468
SmiMI CAYNNNNRTG 1 cut(s) 1341
SmlI CTYRAG 1 cut(s) 468
SmoI CTYRAG 1 cut(s) 468
SpeI ACTAGT 1 cut(s) 935
SphI GCATGC 1 cut(s) 1340
Sse9I AATT 4 cut(s) 416, 543, 993, 1460
SspI AATATT 1 cut(s) 883
SspMI CTAG 3 cut(s) 591, 749, 936
StyD4I CCNGG 6 cut(s) 68, 204, 293, 302, 562, 1053
StyI CCWWGG 2 cut(s) 51, 1411
TaaI ACNGT 3 cut(s) 448, 454, 805
TaiI ACGT 1 cut(s) 1220
TaqI TCGA 8 cut(s) 40, 180, 210, 231, 464, 469, 584, 620
TaqII GACCGA 1 cut(s) 1305
TasI AATT 4 cut(s) 416, 543, 993, 1460
TatI WGTACW 1 cut(s) 506
TauI GCSGC 7 cut(s) 87, 293, 559, 562, 577, 580, 969
TfiI GAWTC 5 cut(s) 255, 389, 1154, 1232, 1300
Tru1I TTAA 3 cut(s) 546, 1314, 1465
Tru9I TTAA 3 cut(s) 546, 1314, 1465
TseFI GTSAC 1 cut(s) 931
TseI GCWGC 4 cut(s) 79, 157, 160, 963
Tsp45I GTSAC 1 cut(s) 931
TspDTI ATGAA 5 cut(s) 723, 1002, 1003, 1167, 1406
TspGWI ACGGA 1 cut(s) 250
VpaK11BI GGWCC 3 cut(s) 190, 195, 315
XapI RAATTY 2 cut(s) 993, 1460
XceI RCATGY 2 cut(s) 1241, 1340
XhoI CTCGAG 1 cut(s) 468
XmiI GTMKAC 2 cut(s) 464, 1248
XmnI GAANNNNTTC 1 cut(s) 901
XspI CTAG 3 cut(s) 591, 749, 936
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.