Mitogen-activated protein kinase kinase kinase 1-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 9 |
| AccB1I | GGYRCC | 1 cut(s) | 1303 |
| AccI | GTMKAC | 1 cut(s) | 1534 |
| AccII | CGCG | 4 cut(s) | 114, 156, 327, 540 |
| AclI | AACGTT | 1 cut(s) | 339 |
| AclWI | GGATC | 1 cut(s) | 894 |
| AcoI | YGGCCR | 2 cut(s) | 115, 544 |
| AcsI | RAATTY | 5 cut(s) | 176, 416, 526, 758, 1259 |
| AcyI | GRCGYC | 3 cut(s) | 6, 78, 1304 |
| AfaI | GTAC | 1 cut(s) | 1035 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 369, 478 |
| AgsI | TTSAA | 5 cut(s) | 490, 926, 1064, 1082, 1507 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1273 |
| AjiI | CACGTC | 1 cut(s) | 1270 |
| AjnI | CCWGG | 2 cut(s) | 897, 1413 |
| AleI | CACNNNNGTG | 1 cut(s) | 519 |
| AluBI | AGCT | 9 cut(s) | 368, 555, 574, 799, 1114, 1132, 1349, 1552, 1604 |
| AluI | AGCT | 9 cut(s) | 368, 555, 574, 799, 1114, 1132, 1349, 1552, 1604 |
| Alw26I | GTCTC | 2 cut(s) | 470, 1198 |
| AlwI | GGATC | 1 cut(s) | 894 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1469, 1555 |
| Ama87I | CYCGRG | 2 cut(s) | 504, 722 |
| AoxI | GGCC | 6 cut(s) | 115, 211, 258, 544, 1397, 1577 |
| ApaI | GGGCCC | 1 cut(s) | 262 |
| ApeKI | GCWGC | 2 cut(s) | 195, 1346 |
| ApoI | RAATTY | 5 cut(s) | 176, 416, 526, 758, 1259 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 161, 193 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 811 |
| AspLEI | GCGC | 3 cut(s) | 114, 1306, 1373 |
| AspS9I | GGNCC | 4 cut(s) | 212, 258, 259, 511 |
| AsuHPI | GGTGA | 3 cut(s) | 271, 1403, 1463 |
| AvaI | CYCGRG | 2 cut(s) | 504, 722 |
| AvaII | GGWCC | 1 cut(s) | 511 |
| AxyI | CCTNAGG | 1 cut(s) | 1308 |
| BaeGI | GKGCMC | 2 cut(s) | 262, 1094 |
| BalI | TGGCCA | 1 cut(s) | 546 |
| BanI | GGYRCC | 1 cut(s) | 1303 |
| BanII | GRGCYC | 1 cut(s) | 262 |
| BbsI | GAAGAC | 1 cut(s) | 225 |
| BbvI | GCAGC | 2 cut(s) | 207, 1358 |
| BccI | CCATC | 5 cut(s) | 236, 658, 842, 1294, 1569 |
| BceAI | ACGGC | 2 cut(s) | 102, 187 |
| BciT130I | CCWGG | 2 cut(s) | 899, 1415 |
| BclI | TGATCA | 1 cut(s) | 670 |
| BcoDI | GTCTC | 2 cut(s) | 470, 1198 |
| BfaI | CTAG | 1 cut(s) | 369 |
| BfoI | RGCGCY | 1 cut(s) | 1307 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 257 |
| BisI | GCNGC | 7 cut(s) | 115, 193, 196, 240, 380, 1347, 1545 |
| BlpI | GCTNAGC | 2 cut(s) | 12, 1548 |
| BlsI | GCNGC | 7 cut(s) | 116, 194, 197, 241, 381, 1348, 1546 |
| Bme1390I | CCNGG | 2 cut(s) | 899, 1415 |
| Bme18I | GGWCC | 1 cut(s) | 511 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1273 |
| BmeT110I | CYCGRG | 2 cut(s) | 504, 722 |
| BmgBI | CACGTC | 1 cut(s) | 1270 |
| BmgT120I | GGNCC | 4 cut(s) | 212, 258, 259, 511 |
| BmiI | GGNNCC | 5 cut(s) | 214, 260, 896, 1305, 1361 |
| BmrFI | CCNGG | 2 cut(s) | 899, 1415 |
| BmrI | ACTGGG | 1 cut(s) | 227 |
| BmsI | GCATC | 3 cut(s) | 967, 1358, 1477 |
| BmuI | ACTGGG | 1 cut(s) | 227 |
| BpiI | GAAGAC | 1 cut(s) | 225 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1171, 1203 |
| BpmI | CTGGAG | 1 cut(s) | 1446 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 12, 1548 |
| BpuEI | CTTGAG | 3 cut(s) | 1148, 1173, 1198 |
| BsaBI | GATNNNNATC | 1 cut(s) | 306 |
| BsaHI | GRCGYC | 3 cut(s) | 6, 78, 1304 |
| BsaI | GGTCTC | 1 cut(s) | 470 |
| BsaJI | CCNNGG | 4 cut(s) | 199, 503, 898, 1363 |
| BsaWI | WCCGGW | 1 cut(s) | 478 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 344, 374 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 369, 478 |
| Bse118I | RCCGGY | 1 cut(s) | 1395 |
| Bse1I | ACTGG | 4 cut(s) | 147, 233, 888, 1463 |
| Bse21I | CCTNAGG | 1 cut(s) | 1308 |
| Bse3DI | GCAATG | 1 cut(s) | 1612 |
| Bse8I | GATNNNNATC | 1 cut(s) | 306 |
| BseBI | CCWGG | 2 cut(s) | 899, 1415 |
| BseDI | CCNNGG | 4 cut(s) | 199, 503, 898, 1363 |
| BseJI | GATNNNNATC | 1 cut(s) | 306 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 369, 478 |
| BseMI | GCAATG | 1 cut(s) | 1612 |
| BseMII | CTCAG | 5 cut(s) | 332, 984, 1299, 1562, 1608 |
| BseNI | ACTGG | 4 cut(s) | 147, 233, 888, 1463 |
| BseRI | GAGGAG | 3 cut(s) | 584, 1449, 1599 |
| BseSI | GKGCMC | 2 cut(s) | 262, 1094 |
| BseX3I | CGGCCG | 1 cut(s) | 115 |
| BseXI | GCAGC | 2 cut(s) | 207, 1358 |
| BseYI | CCCAGC | 2 cut(s) | 252, 1600 |
| Bsh1236I | CGCG | 4 cut(s) | 114, 156, 327, 540 |
| Bsh1285I | CGRYCG | 2 cut(s) | 46, 118 |
| BshFI | GGCC | 6 cut(s) | 117, 213, 260, 546, 1399, 1579 |
| BshNI | GGYRCC | 1 cut(s) | 1303 |
| BsiEI | CGRYCG | 2 cut(s) | 46, 118 |
| BsiHKCI | CYCGRG | 2 cut(s) | 504, 722 |
| BsiSI | CCGG | 3 cut(s) | 210, 479, 1396 |
| BslFI | GGGAC | 3 cut(s) | 835, 1300, 1583 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 369, 478 |
| BsmAI | GTCTC | 2 cut(s) | 470, 1198 |
| BsmFI | GGGAC | 3 cut(s) | 835, 1300, 1583 |
| BsnI | GGCC | 6 cut(s) | 117, 213, 260, 546, 1399, 1579 |
| Bso31I | GGTCTC | 1 cut(s) | 470 |
| BsoBI | CYCGRG | 2 cut(s) | 504, 722 |
| Bsp120I | GGGCCC | 1 cut(s) | 258 |
| Bsp1286I | GDGCHC | 2 cut(s) | 262, 1094 |
| Bsp1407I | TGTACA | 1 cut(s) | 1033 |
| Bsp143I | GATC | 4 cut(s) | 301, 670, 886, 1240 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 12, 1548 |
| BspANI | GGCC | 6 cut(s) | 117, 213, 260, 546, 1399, 1579 |
| BspCNI | CTCAG | 5 cut(s) | 331, 983, 1300, 1561, 1607 |
| BspFNI | CGCG | 4 cut(s) | 114, 156, 327, 540 |
| BspHI | TCATGA | 1 cut(s) | 1237 |
| BspLI | GGNNCC | 5 cut(s) | 214, 260, 896, 1305, 1361 |
| BspPI | GGATC | 1 cut(s) | 894 |
| BspT107I | GGYRCC | 1 cut(s) | 1303 |
| BspTNI | GGTCTC | 1 cut(s) | 470 |
| BsrDI | GCAATG | 1 cut(s) | 1612 |
| BsrFI | RCCGGY | 1 cut(s) | 1395 |
| BsrGI | TGTACA | 1 cut(s) | 1033 |
| BsrI | ACTGG | 4 cut(s) | 147, 233, 888, 1463 |
| BssAI | RCCGGY | 1 cut(s) | 1395 |
| BssECI | CCNNGG | 4 cut(s) | 199, 503, 898, 1363 |
| BssMI | GATC | 4 cut(s) | 301, 670, 886, 1240 |
| BssNI | GRCGYC | 3 cut(s) | 6, 78, 1304 |
| BssT1I | CCWWGG | 1 cut(s) | 1363 |
| Bst2UI | CCWGG | 2 cut(s) | 899, 1415 |
| Bst4CI | ACNGT | 4 cut(s) | 389, 826, 944, 1622 |
| Bst6I | CTCTTC | 1 cut(s) | 561 |
| BstACI | GRCGYC | 3 cut(s) | 6, 78, 1304 |
| BstAUI | TGTACA | 1 cut(s) | 1033 |
| BstC8I | GCNNGC | 8 cut(s) | 258, 262, 473, 576, 1344, 1397, 1424, 1428 |
| BstDEI | CTNAG | 8 cut(s) | 12, 318, 970, 1110, 1308, 1322, 1548, 1594 |
| BstFNI | CGCG | 4 cut(s) | 114, 156, 327, 540 |
| BstH2I | RGCGCY | 1 cut(s) | 1307 |
| BstHHI | GCGC | 3 cut(s) | 114, 1306, 1373 |
| BstKTI | GATC | 4 cut(s) | 304, 673, 889, 1243 |
| BstMAI | GTCTC | 2 cut(s) | 470, 1198 |
| BstMBI | GATC | 4 cut(s) | 301, 670, 886, 1240 |
| BstMCI | CGRYCG | 2 cut(s) | 46, 118 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 162, 171, 248, 257, 552, 584, 1368 |
| BstNI | CCWGG | 2 cut(s) | 899, 1415 |
| BstNSI | RCATGY | 1 cut(s) | 1426 |
| BstSCI | CCNGG | 2 cut(s) | 897, 1413 |
| BstSLI | GKGCMC | 2 cut(s) | 262, 1094 |
| BstUI | CGCG | 4 cut(s) | 114, 156, 327, 540 |
| BstV1I | GCAGC | 2 cut(s) | 207, 1358 |
| BstV2I | GAAGAC | 1 cut(s) | 225 |
| BstZI | CGGCCG | 1 cut(s) | 115 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1308 |
| BsuRI | GGCC | 6 cut(s) | 117, 213, 260, 546, 1399, 1579 |
| BtgZI | GCGATG | 1 cut(s) | 1500 |
| BtrI | CACGTC | 1 cut(s) | 1270 |
| BtsIMutI | CAGTG | 1 cut(s) | 394 |
| Cac8I | GCNNGC | 8 cut(s) | 258, 262, 473, 576, 1344, 1397, 1424, 1428 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1469, 1555 |
| CciI | TCATGA | 1 cut(s) | 1237 |
| CfoI | GCGC | 3 cut(s) | 114, 1306, 1373 |
| Cfr10I | RCCGGY | 1 cut(s) | 1395 |
| Cfr13I | GGNCC | 4 cut(s) | 212, 258, 259, 511 |
| CseI | GACGC | 2 cut(s) | 86, 145 |
| Csp6I | GTAC | 1 cut(s) | 1034 |
| CviAII | CATG | 4 cut(s) | 548, 1067, 1238, 1423 |
| CviQI | GTAC | 1 cut(s) | 1034 |
| DdeI | CTNAG | 8 cut(s) | 12, 318, 970, 1110, 1308, 1322, 1548, 1594 |
| DinI | GGCGCC | 1 cut(s) | 1305 |
| DpnI | GATC | 4 cut(s) | 303, 672, 888, 1242 |
| DpnII | GATC | 4 cut(s) | 301, 670, 886, 1240 |
| DraI | TTTAAA | 1 cut(s) | 22 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1273 |
| EaeI | YGGCCR | 2 cut(s) | 115, 544 |
| EagI | CGGCCG | 1 cut(s) | 115 |
| Eam1104I | CTCTTC | 1 cut(s) | 561 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1273 |
| EarI | CTCTTC | 1 cut(s) | 561 |
| EciI | GGCGGA | 2 cut(s) | 458, 781 |
| EclXI | CGGCCG | 1 cut(s) | 115 |
| Eco130I | CCWWGG | 1 cut(s) | 1363 |
| Eco24I | GRGCYC | 1 cut(s) | 262 |
| Eco31I | GGTCTC | 1 cut(s) | 470 |
| Eco47I | GGWCC | 1 cut(s) | 511 |
| Eco52I | CGGCCG | 1 cut(s) | 115 |
| Eco81I | CCTNAGG | 1 cut(s) | 1308 |
| Eco88I | CYCGRG | 2 cut(s) | 504, 722 |
| EcoRI | GAATTC | 1 cut(s) | 176 |
| EcoRII | CCWGG | 2 cut(s) | 897, 1413 |
| EcoT14I | CCWWGG | 1 cut(s) | 1363 |
| EcoT38I | GRGCYC | 1 cut(s) | 262 |
| EgeI | GGCGCC | 1 cut(s) | 1305 |
| EheI | GGCGCC | 1 cut(s) | 1305 |
| ErhI | CCWWGG | 1 cut(s) | 1363 |
| FaeI | CATG | 4 cut(s) | 551, 1070, 1241, 1426 |
| FaqI | GGGAC | 3 cut(s) | 835, 1300, 1583 |
| FatI | CATG | 4 cut(s) | 547, 1066, 1237, 1422 |
| FauI | CCCGC | 5 cut(s) | 249, 269, 480, 1214, 1400 |
| FauNDI | CATATG | 1 cut(s) | 1355 |
| FbaI | TGATCA | 1 cut(s) | 670 |
| FblI | GTMKAC | 1 cut(s) | 1534 |
| Fnu4HI | GCNGC | 7 cut(s) | 115, 193, 196, 240, 380, 1347, 1545 |
| FriOI | GRGCYC | 1 cut(s) | 262 |
| Fsp4HI | GCNGC | 7 cut(s) | 115, 193, 196, 240, 380, 1347, 1545 |
| FspBI | CTAG | 1 cut(s) | 369 |
| GlaI | GCGC | 3 cut(s) | 113, 1305, 1372 |
| GluI | GCNGC | 7 cut(s) | 115, 193, 196, 240, 380, 1347, 1545 |
| GsaI | CCCAGC | 2 cut(s) | 256, 1604 |
| GsuI | CTGGAG | 1 cut(s) | 1446 |
| HaeII | RGCGCY | 1 cut(s) | 1307 |
| HaeIII | GGCC | 6 cut(s) | 117, 213, 260, 546, 1399, 1579 |
| HapII | CCGG | 3 cut(s) | 210, 479, 1396 |
| HgaI | GACGC | 2 cut(s) | 86, 145 |
| HhaI | GCGC | 3 cut(s) | 114, 1306, 1373 |
| Hin1I | GRCGYC | 3 cut(s) | 6, 78, 1304 |
| Hin1II | CATG | 4 cut(s) | 551, 1070, 1241, 1426 |
| Hin6I | GCGC | 3 cut(s) | 112, 1304, 1371 |
| HinP1I | GCGC | 3 cut(s) | 112, 1304, 1371 |
| HincII | GTYRAC | 5 cut(s) | 343, 1227, 1389, 1522, 1535 |
| HindII | GTYRAC | 5 cut(s) | 343, 1227, 1389, 1522, 1535 |
| HinfI | GANTC | 9 cut(s) | 307, 535, 602, 769, 852, 996, 1024, 1184, 1327 |
| HpaI | GTTAAC | 2 cut(s) | 1389, 1522 |
| HpaII | CCGG | 3 cut(s) | 210, 479, 1396 |
| HphI | GGTGA | 3 cut(s) | 271, 1403, 1463 |
| Hpy188III | TCNNGA | 6 cut(s) | 506, 993, 1177, 1238, 1469, 1632 |
| Hpy99I | CGWCG | 2 cut(s) | 11, 80 |
| HpyAV | CCTTC | 5 cut(s) | 105, 137, 827, 1412, 1569 |
| HpyCH4III | ACNGT | 4 cut(s) | 389, 826, 944, 1622 |
| HpyCH4IV | ACGT | 6 cut(s) | 6, 25, 276, 339, 345, 1269 |
| HpyCH4V | TGCA | 8 cut(s) | 578, 680, 1154, 1281, 1346, 1426, 1490, 1617 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 162, 171, 248, 257, 552, 584, 1368 |
| HpyF3I | CTNAG | 8 cut(s) | 12, 318, 970, 1110, 1308, 1322, 1548, 1594 |
| HpySE526I | ACGT | 6 cut(s) | 6, 25, 276, 339, 345, 1269 |
| Hsp92I | GRCGYC | 3 cut(s) | 6, 78, 1304 |
| Hsp92II | CATG | 4 cut(s) | 551, 1070, 1241, 1426 |
| HspAI | GCGC | 3 cut(s) | 112, 1304, 1371 |
| KasI | GGCGCC | 1 cut(s) | 1303 |
| KroI | GCCGGC | 1 cut(s) | 1395 |
| KroNI | GCCGGC | 1 cut(s) | 1397 |
| Ksp22I | TGATCA | 1 cut(s) | 670 |
| KspAI | GTTAAC | 2 cut(s) | 1389, 1522 |
| Kzo9I | GATC | 4 cut(s) | 301, 670, 886, 1240 |
| LmnI | GCTCC | 3 cut(s) | 571, 894, 1359 |
| Lsp1109I | GCAGC | 2 cut(s) | 207, 1358 |
| LweI | GCATC | 3 cut(s) | 967, 1358, 1477 |
| MaeI | CTAG | 1 cut(s) | 369 |
| MaeII | ACGT | 6 cut(s) | 6, 25, 276, 339, 345, 1269 |
| MaeIII | GTNAC | 3 cut(s) | 277, 321, 1135 |
| MalI | GATC | 4 cut(s) | 303, 672, 888, 1242 |
| MboI | GATC | 4 cut(s) | 301, 670, 886, 1240 |
| MboII | GAAGA | 6 cut(s) | 230, 367, 578, 1005, 1114, 1226 |
| MhlI | GDGCHC | 2 cut(s) | 262, 1094 |
| MlsI | TGGCCA | 1 cut(s) | 546 |
| MluCI | AATT | 7 cut(s) | 17, 176, 416, 456, 526, 758, 1259 |
| MluNI | TGGCCA | 1 cut(s) | 546 |
| Mly113I | GGCGCC | 1 cut(s) | 1304 |
| MlyI | GAGTC | 1 cut(s) | 778 |
| MmeI | TCCRAC | 4 cut(s) | 159, 297, 663, 943 |
| Mox20I | TGGCCA | 1 cut(s) | 546 |
| MroNI | GCCGGC | 1 cut(s) | 1395 |
| MroXI | GAANNNNTTC | 1 cut(s) | 811 |
| MscI | TGGCCA | 1 cut(s) | 546 |
| MslI | CAYNNNNRTG | 1 cut(s) | 519 |
| Msp20I | TGGCCA | 1 cut(s) | 546 |
| MspA1I | CMGCKG | 2 cut(s) | 256, 1349 |
| MspI | CCGG | 3 cut(s) | 210, 479, 1396 |
| MspR9I | CCNGG | 2 cut(s) | 899, 1415 |
| MvaI | CCWGG | 2 cut(s) | 899, 1415 |
| MvnI | CGCG | 4 cut(s) | 114, 156, 327, 540 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 162, 171, 248, 257, 552, 584, 1368 |
| NaeI | GCCGGC | 1 cut(s) | 1397 |
| NarI | GGCGCC | 1 cut(s) | 1304 |
| NdeI | CATATG | 1 cut(s) | 1355 |
| NdeII | GATC | 4 cut(s) | 301, 670, 886, 1240 |
| NgoMIV | GCCGGC | 1 cut(s) | 1395 |
| NlaIII | CATG | 4 cut(s) | 551, 1070, 1241, 1426 |
| NlaIV | GGNNCC | 5 cut(s) | 214, 260, 896, 1305, 1361 |
| NmuCI | GTSAC | 1 cut(s) | 277 |
| NspI | RCATGY | 1 cut(s) | 1426 |
| OliI | CACNNNNGTG | 1 cut(s) | 519 |
| PaeI | GCATGC | 1 cut(s) | 1426 |
| PagI | TCATGA | 1 cut(s) | 1237 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 177 |
| PdiI | GCCGGC | 1 cut(s) | 1397 |
| PdmI | GAANNNNTTC | 1 cut(s) | 811 |
| PfeI | GAWTC | 8 cut(s) | 307, 535, 602, 852, 996, 1024, 1184, 1327 |
| PkrI | GCNGC | 7 cut(s) | 116, 194, 197, 241, 381, 1348, 1546 |
| PleI | GAGTC | 1 cut(s) | 777 |
| PluTI | GGCGCC | 1 cut(s) | 1307 |
| PpsI | GAGTC | 1 cut(s) | 777 |
| Psp1406I | AACGTT | 1 cut(s) | 339 |
| Psp6I | CCWGG | 2 cut(s) | 897, 1413 |
| PspFI | CCCAGC | 2 cut(s) | 252, 1600 |
| PspGI | CCWGG | 2 cut(s) | 897, 1413 |
| PspN4I | GGNNCC | 5 cut(s) | 214, 260, 896, 1305, 1361 |
| PspOMI | GGGCCC | 1 cut(s) | 258 |
| PspPI | GGNCC | 4 cut(s) | 212, 258, 259, 511 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 56, 88 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1469, 1555 |
| PvuII | CAGCTG | 1 cut(s) | 1349 |
| RsaI | GTAC | 1 cut(s) | 1035 |
| RsaNI | GTAC | 1 cut(s) | 1034 |
| RseI | CAYNNNNRTG | 1 cut(s) | 519 |
| SalI | GTCGAC | 1 cut(s) | 1533 |
| SatI | GCNGC | 7 cut(s) | 115, 193, 196, 240, 380, 1347, 1545 |
| Sau3AI | GATC | 4 cut(s) | 301, 670, 886, 1240 |
| Sau96I | GGNCC | 4 cut(s) | 212, 258, 259, 511 |
| SchI | GAGTC | 1 cut(s) | 778 |
| ScrFI | CCNGG | 2 cut(s) | 899, 1415 |
| SduI | GDGCHC | 2 cut(s) | 262, 1094 |
| SfaNI | GCATC | 3 cut(s) | 967, 1358, 1477 |
| SfoI | GGCGCC | 1 cut(s) | 1305 |
| SinI | GGWCC | 1 cut(s) | 511 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 519 |
| SmlI | CTYRAG | 3 cut(s) | 1127, 1177, 1188 |
| SmoI | CTYRAG | 3 cut(s) | 1127, 1177, 1188 |
| SphI | GCATGC | 1 cut(s) | 1426 |
| Sse9I | AATT | 7 cut(s) | 17, 176, 416, 456, 526, 758, 1259 |
| SspDI | GGCGCC | 1 cut(s) | 1303 |
| SspMI | CTAG | 1 cut(s) | 369 |
| StyD4I | CCNGG | 2 cut(s) | 897, 1413 |
| StyI | CCWWGG | 1 cut(s) | 1363 |
| TaaI | ACNGT | 4 cut(s) | 389, 826, 944, 1622 |
| TaiI | ACGT | 6 cut(s) | 9, 28, 279, 342, 348, 1272 |
| TaqI | TCGA | 6 cut(s) | 42, 69, 180, 384, 933, 1534 |
| TasI | AATT | 7 cut(s) | 17, 176, 416, 456, 526, 758, 1259 |
| TatI | WGTACW | 1 cut(s) | 1033 |
| TauI | GCSGC | 5 cut(s) | 117, 195, 242, 382, 1547 |
| TfiI | GAWTC | 8 cut(s) | 307, 535, 602, 852, 996, 1024, 1184, 1327 |
| TscAI | CASTG | 1 cut(s) | 394 |
| TseFI | GTSAC | 1 cut(s) | 277 |
| TseI | GCWGC | 2 cut(s) | 195, 1346 |
| Tsp45I | GTSAC | 1 cut(s) | 277 |
| TspDTI | ATGAA | 6 cut(s) | 579, 597, 846, 1119, 1226, 1489 |
| TspRI | CASTG | 1 cut(s) | 394 |
| VpaK11BI | GGWCC | 1 cut(s) | 511 |
| XapI | RAATTY | 5 cut(s) | 176, 416, 526, 758, 1259 |
| XceI | RCATGY | 1 cut(s) | 1426 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 240, 1470 |
| XmiI | GTMKAC | 1 cut(s) | 1534 |
| XmnI | GAANNNNTTC | 1 cut(s) | 811 |
| XspI | CTAG | 1 cut(s) | 369 |
| ZraI | GACGTC | 1 cut(s) | 7 |