RLG00000013468

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000243
Physical Location & Seq
Forward (+)
32021219 .. 32022698
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013468

Sequence Viewer

Length: 333 bp
ATGGGTTCGAATCAGACGTTTCGGTACAAAACTGATCGGTCCGAATCAGACCCAAAAGCCAGTTTCGCCTTTGCCTACTTCGTCATCATCTTCATCCTCCTTGGTGGAGTCTTCGGCGACCCTGACGTGTTCAAAGCATTAGATAATAAAGGCCGCATCGACCATTTCAGAAGGCTACCATACTTGAATTTCCTAGTGGGAATGCCGCGCTGGCATGGAAGATTGGAAAGACAGGAGCCTCCTCTGGTTCCTGATTGTCTGTTGATAGAGGCACAAGGTTTCATCCATCAATGCCTACAAGTTGATCCAAACAATCGCCCCAGTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

111

Amino Acids

12.6

Weight (kDa)

7.83

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 208
AciI CCGC 2 cut(s) 154, 206
AclWI GGATC 1 cut(s) 299
AcsI RAATTY 1 cut(s) 187
AfaI GTAC 1 cut(s) 26
AflIII ACRYGT 1 cut(s) 126
AgsI TTSAA 2 cut(s) 133, 187
AjiI CACGTC 1 cut(s) 127
AlwI GGATC 1 cut(s) 299
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 1 cut(s) 187
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 39
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 313
BccI CCATC 1 cut(s) 294
BfaI CTAG 1 cut(s) 194
BglI GCCNNNNNGGC 1 cut(s) 211
BisI GCNGC 3 cut(s) 154, 206, 327
BlsI GCNGC 3 cut(s) 155, 207, 328
Bme18I GGWCC 1 cut(s) 39
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 2 cut(s) 237, 249
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 165
BmuI ACTGGG 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 103
Bpu14I TTCGAA 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 100
Bse1I ACTGG 2 cut(s) 60, 321
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 2 cut(s) 93, 282
BseNI ACTGG 2 cut(s) 60, 321
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 313
Bsh1236I CGCG 1 cut(s) 208
BshFI GGCC 1 cut(s) 153
BsmI GAATGC 1 cut(s) 207
BsnI GGCC 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 8
Bsp143I GATC 2 cut(s) 34, 304
BspACI CCGC 2 cut(s) 154, 206
BspANI GGCC 1 cut(s) 153
BspFNI CGCG 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 237, 249
BspPI GGATC 1 cut(s) 299
BspT104I TTCGAA 1 cut(s) 8
BsrI ACTGG 2 cut(s) 60, 321
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 2 cut(s) 34, 304
BssT1I CCWWGG 1 cut(s) 100
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 212
BstDEI CTNAG 1 cut(s) 330
BstF5I GGATG 2 cut(s) 93, 282
BstFNI CGCG 1 cut(s) 208
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 2 cut(s) 37, 307
BstMBI GATC 2 cut(s) 34, 304
BstMWI GCNNNNNNNGC 2 cut(s) 65, 211
BstUI CGCG 1 cut(s) 208
BstV1I GCAGC 1 cut(s) 313
BstV2I GAAGAC 1 cut(s) 103
BsuRI GGCC 1 cut(s) 153
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 2 cut(s) 93, 282
BtsIMutI CAGTG 1 cut(s) 328
Cac8I GCNNGC 1 cut(s) 212
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 39
CpoI CGGWCCG 1 cut(s) 39
Csp6I GTAC 1 cut(s) 25
CspI CGGWCCG 1 cut(s) 39
CviAII CATG 1 cut(s) 215
CviJI RGCY 4 cut(s) 59, 153, 175, 238
CviKI_1 RGCY 4 cut(s) 59, 153, 175, 238
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 1 cut(s) 330
DpnI GATC 2 cut(s) 36, 306
DpnII GATC 2 cut(s) 34, 304
Eco130I CCWWGG 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 1 cut(s) 218
FaiI YATR 2 cut(s) 181, 216
FatI CATG 1 cut(s) 214
Fnu4HI GCNGC 3 cut(s) 154, 206, 327
FokI GGATG 2 cut(s) 80, 269
Fsp4HI GCNGC 3 cut(s) 154, 206, 327
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 209
GluI GCNGC 3 cut(s) 154, 206, 327
HaeIII GGCC 1 cut(s) 153
HhaI GCGC 1 cut(s) 210
Hin1II CATG 1 cut(s) 218
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HinfI GANTC 3 cut(s) 10, 44, 108
Hpy188I TCNGA 4 cut(s) 15, 43, 49, 170
Hpy188III TCNNGA 1 cut(s) 251
HpyAV CCTTC 1 cut(s) 165
HpyCH4IV ACGT 2 cut(s) 17, 126
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 211
HpyF3I CTNAG 1 cut(s) 330
HpySE526I ACGT 2 cut(s) 17, 126
Hsp92II CATG 1 cut(s) 218
HspAI GCGC 1 cut(s) 208
Kzo9I GATC 2 cut(s) 34, 304
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 6 cut(s) 73, 135, 196, 218, 230, 264
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 1 cut(s) 165
MaeI CTAG 1 cut(s) 194
MaeII ACGT 2 cut(s) 17, 126
MalI GATC 2 cut(s) 36, 306
MboI GATC 2 cut(s) 34, 304
MboII GAAGA 3 cut(s) 82, 103, 231
MluCI AATT 1 cut(s) 187
MlyI GAGTC 1 cut(s) 117
MnlI CCTC 4 cut(s) 107, 249, 252, 262
Mva1269I GAATGC 1 cut(s) 207
MvnI CGCG 1 cut(s) 208
MwoI GCNNNNNNNGC 2 cut(s) 65, 211
NdeII GATC 2 cut(s) 34, 304
NlaIII CATG 1 cut(s) 218
NlaIV GGNNCC 2 cut(s) 237, 249
NspV TTCGAA 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 1 cut(s) 207
PfeI GAWTC 2 cut(s) 10, 44
PkrI GCNGC 3 cut(s) 155, 207, 328
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PspN4I GGNNCC 2 cut(s) 237, 249
PspPI GGNCC 1 cut(s) 39
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
Rsr2I CGGWCCG 1 cut(s) 39
RsrII CGGWCCG 1 cut(s) 39
SatI GCNGC 3 cut(s) 154, 206, 327
Sau3AI GATC 2 cut(s) 34, 304
Sau96I GGNCC 1 cut(s) 39
SchI GAGTC 1 cut(s) 117
SetI ASST 3 cut(s) 20, 129, 280
SfaNI GCATC 1 cut(s) 165
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 1 cut(s) 39
Sse9I AATT 1 cut(s) 187
SsiI CCGC 2 cut(s) 154, 206
SspMI CTAG 1 cut(s) 194
StyI CCWWGG 1 cut(s) 100
TaiI ACGT 2 cut(s) 20, 129
TaqI TCGA 2 cut(s) 8, 159
TaqII GACCGA 1 cut(s) 27
TasI AATT 1 cut(s) 187
TauI GCSGC 2 cut(s) 156, 208
TfiI GAWTC 2 cut(s) 10, 44
TscAI CASTG 1 cut(s) 328
TseI GCWGC 1 cut(s) 326
TspDTI ATGAA 2 cut(s) 82, 271
TspRI CASTG 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 39
XapI RAATTY 1 cut(s) 187
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.