Rorug05G0465900

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000243
Physical Location & Seq
Reverse (-)
64166293 .. 64167226
934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0465900.1

Sequence Viewer

Length: 708 bp
ATGCCGGCCACCGCAGGAAGGGTTCGCATGCCCCCAAACAATCGGGTACACAGTAGTGTTGCCCTTCAAACTCATGGTATATGGCAGAGTACAATTGGGTACGATCCCTATGCACCTCAGCCGAATTTGTCAAACTCAAAACCAGATGCTGAGAATGCATATGCTAGCTATCAAGGCCTTCTCCAACTCGCTCGTATAACTGGGCCGAATTCTGACGAGGTTCGTGGGGCGTGCAAGAGGTGCGGCCGGGTTGGGCACCTCACCTATCAATGTAGGAATTTTCTGAGTGTTAAGGACAAGGAGGAGCCTGAAGCTATCCAGGCTGATGTTGCTTCTGAGTTGGGCAAGTTGAAGGGGAAGCTGGGTAAAGCGAAGGGAAAAGACGTTGTTAAGACTGATAGTGAGGAGGAGGATGAAGACATTGAGAGTTCAGATTCGGCCTATGATTCTGATATGGAGAGGCTCATTGCTCAAAGATATGGGAAAAAGAGTTTTCGAAAGGAGAAGCTGAAAAAGAAGAACAAGGACTCGGATGTTGATGGTTCAGATACAGATTCTGGGGAGAGGAAGAAGAGACGAAGGTCGAAGAAGAGGAGAAGCAGAAAGAGGAAACATGATGATTCAGATGATTCTGAAAGGGATAGGAAGAAAAGAAGAAAGGAGAAACGGAGAAAGAGAGATGAGTCCTCGGATGAGGATGATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

235

Amino Acids

26.8

Weight (kDa)

9.49

Isoelectric Point (pI)

66.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_3 PF13917 74 - 93 1e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 255
AciI CCGC 2 cut(s) 12, 243
AclWI GGATC 1 cut(s) 98
AcoI YGGCCR 2 cut(s) 6, 244
AcsI RAATTY 3 cut(s) 124, 208, 277
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 3 cut(s) 48, 91, 101
AfiI CCNNNNNNNGG 1 cut(s) 18
AgsI TTSAA 2 cut(s) 68, 352
AjnI CCWGG 1 cut(s) 318
AleI CACNNNNGTG 1 cut(s) 54
AluBI AGCT 4 cut(s) 168, 314, 361, 508
AluI AGCT 4 cut(s) 168, 314, 361, 508
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 1 cut(s) 98
AlwNI CAGNNNCTG 2 cut(s) 149, 557
AoxI GGCC 5 cut(s) 6, 175, 203, 244, 438
ApoI RAATTY 3 cut(s) 124, 208, 277
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 253
AsuII TTCGAA 1 cut(s) 496
AsuNHI GCTAGC 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 258
BanI GGYRCC 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 423
BbvCI CCTCAGC 1 cut(s) 117
BccI CCATC 1 cut(s) 533
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 1 cut(s) 320
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 1 cut(s) 165
BisI GCNGC 1 cut(s) 244
BlsI GCNGC 1 cut(s) 245
Bme1390I CCNGG 2 cut(s) 248, 320
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 2 cut(s) 257, 306
BmrFI CCNGG 2 cut(s) 248, 320
BmrI ACTGGG 1 cut(s) 210
BmsI GCATC 1 cut(s) 136
BmtI GCTAGC 1 cut(s) 168
BmuI ACTGGG 1 cut(s) 210
BoxI GACNNNNGTC 1 cut(s) 580
BpiI GAAGAC 1 cut(s) 423
Bpu10I CCTNAGC 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 496
BpuMI CCSGG 1 cut(s) 248
BsaJI CCNNGG 1 cut(s) 687
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 465
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 687
BseGI GGATG 4 cut(s) 418, 538, 697, 703
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMI GCAATG 1 cut(s) 465
BseMII CTCAG 4 cut(s) 131, 141, 275, 327
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 4 cut(s) 317, 419, 422, 607
BseSI GKGCMC 1 cut(s) 258
BseX3I CGGCCG 1 cut(s) 244
BseYI CCCAGC 1 cut(s) 361
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 5 cut(s) 8, 177, 205, 246, 440
BshNI GGYRCC 1 cut(s) 255
BsiEI CGRYCG 1 cut(s) 247
BsiSI CCGG 2 cut(s) 5, 247
BslI CCNNNNNNNGG 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 568
BsmBI CGTCTC 1 cut(s) 568
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 5 cut(s) 8, 177, 205, 246, 440
Bsp119I TTCGAA 1 cut(s) 496
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 2 cut(s) 103, 700
BspACI CCGC 2 cut(s) 12, 243
BspANI GGCC 5 cut(s) 8, 177, 205, 246, 440
BspCNI CTCAG 4 cut(s) 130, 142, 276, 328
BspLI GGNNCC 2 cut(s) 257, 306
BspOI GCTAGC 1 cut(s) 168
BspPI GGATC 1 cut(s) 98
BspT104I TTCGAA 1 cut(s) 496
BspT107I GGYRCC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 465
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 687
BssMI GATC 2 cut(s) 103, 700
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 53
Bst6I CTCTTC 2 cut(s) 566, 584
BstAPI GCANNNNNTGC 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 496
BstC8I GCNNGC 4 cut(s) 6, 29, 166, 232
BstDEI CTNAG 4 cut(s) 117, 150, 284, 336
BstF5I GGATG 4 cut(s) 418, 538, 697, 703
BstKTI GATC 2 cut(s) 106, 703
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 2 cut(s) 103, 700
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 5 cut(s) 155, 174, 240, 320, 329
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 1 cut(s) 31
BstPAI GACNNNNGTC 1 cut(s) 580
BstSCI CCNGG 2 cut(s) 246, 318
BstSLI GKGCMC 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 423
BstZI CGGCCG 1 cut(s) 244
BsuRI GGCC 5 cut(s) 8, 177, 205, 246, 440
BtsCI GGATG 4 cut(s) 418, 538, 697, 703
Cac8I GCNNGC 4 cut(s) 6, 29, 166, 232
CaiI CAGNNNCTG 2 cut(s) 149, 557
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 203
Csp6I GTAC 3 cut(s) 47, 90, 100
CviAII CATG 3 cut(s) 28, 74, 614
CviQI GTAC 3 cut(s) 47, 90, 100
DdeI CTNAG 4 cut(s) 117, 150, 284, 336
DpnI GATC 2 cut(s) 105, 702
DpnII GATC 2 cut(s) 103, 700
EaeI YGGCCR 2 cut(s) 6, 244
EagI CGGCCG 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 566, 584
EarI CTCTTC 2 cut(s) 566, 584
EclXI CGGCCG 1 cut(s) 244
Eco147I AGGCCT 1 cut(s) 177
Eco52I CGGCCG 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 330
EcoRI GAATTC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 318
EcoT22I ATGCAT 1 cut(s) 160
Esp3I CGTCTC 1 cut(s) 568
FaeI CATG 3 cut(s) 31, 77, 617
FatI CATG 3 cut(s) 27, 73, 613
FauNDI CATATG 1 cut(s) 160
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 1 cut(s) 244
FokI GGATG 3 cut(s) 425, 545, 704
Fsp4HI GCNGC 1 cut(s) 244
FspBI CTAG 1 cut(s) 165
GluI GCNGC 1 cut(s) 244
GsaI CCCAGC 1 cut(s) 365
HaeIII GGCC 5 cut(s) 8, 177, 205, 246, 440
HapII CCGG 2 cut(s) 5, 247
Hin1II CATG 3 cut(s) 31, 77, 617
HinfI GANTC 7 cut(s) 434, 446, 527, 554, 620, 629, 683
HpaII CCGG 2 cut(s) 5, 247
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 1 cut(s) 49
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 6 cut(s) 12, 74, 188, 346, 367, 573
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 3 cut(s) 113, 158, 234
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 174, 240, 320, 329
HpyF3I CTNAG 4 cut(s) 117, 150, 284, 336
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 3 cut(s) 31, 77, 617
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 2 cut(s) 103, 700
LmnI GCTCC 1 cut(s) 304
LpnPI CCDG 9 cut(s) 18, 156, 186, 260, 305, 321, 332, 347, 543
LweI GCATC 1 cut(s) 136
MaeI CTAG 1 cut(s) 165
MaeII ACGT 1 cut(s) 384
MalI GATC 2 cut(s) 105, 702
MboI GATC 2 cut(s) 103, 700
MboII GAAGA 8 cut(s) 428, 529, 580, 583, 598, 601, 658, 666
MfeI CAATTG 1 cut(s) 93
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 4 cut(s) 93, 124, 208, 277
MlyI GAGTC 2 cut(s) 521, 692
MmeI TCCRAC 1 cut(s) 208
Mph1103I ATGCAT 1 cut(s) 160
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 2 cut(s) 291, 390
MslI CAYNNNNRTG 1 cut(s) 54
MspI CCGG 2 cut(s) 5, 247
MspR9I CCNGG 2 cut(s) 248, 320
MunI CAATTG 1 cut(s) 93
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 5 cut(s) 155, 174, 240, 320, 329
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 248
NdeI CATATG 1 cut(s) 160
NdeII GATC 2 cut(s) 103, 700
NgoMIV GCCGGC 1 cut(s) 4
NheI GCTAGC 1 cut(s) 164
NlaIII CATG 3 cut(s) 31, 77, 617
NlaIV GGNNCC 2 cut(s) 257, 306
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 1 cut(s) 31
NspV TTCGAA 1 cut(s) 496
OliI CACNNNNGTG 1 cut(s) 54
PaeI GCATGC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 177
PctI GAATGC 1 cut(s) 160
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 5 cut(s) 434, 446, 554, 620, 629
PkrI GCNGC 1 cut(s) 245
PleI GAGTC 2 cut(s) 521, 691
PpsI GAGTC 2 cut(s) 521, 691
PshAI GACNNNNGTC 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 318
PspFI CCCAGC 1 cut(s) 361
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 257, 306
PspPI GGNCC 1 cut(s) 203
PstNI CAGNNNCTG 2 cut(s) 149, 557
RsaI GTAC 3 cut(s) 48, 91, 101
RsaNI GTAC 3 cut(s) 47, 90, 100
RseI CAYNNNNRTG 1 cut(s) 54
SaqAI TTAA 2 cut(s) 291, 390
SatI GCNGC 1 cut(s) 244
Sau3AI GATC 2 cut(s) 103, 700
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 2 cut(s) 521, 692
ScrFI CCNGG 2 cut(s) 248, 320
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 1 cut(s) 136
SfuI TTCGAA 1 cut(s) 496
SmiMI CAYNNNNRTG 1 cut(s) 54
SphI GCATGC 1 cut(s) 31
Sse9I AATT 4 cut(s) 93, 124, 208, 277
SseBI AGGCCT 1 cut(s) 177
SsiI CCGC 2 cut(s) 12, 243
SspMI CTAG 1 cut(s) 165
StuI AGGCCT 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 246, 318
TaaI ACNGT 1 cut(s) 53
TaiI ACGT 1 cut(s) 387
TaqI TCGA 2 cut(s) 496, 584
TasI AATT 4 cut(s) 93, 124, 208, 277
TatI WGTACW 1 cut(s) 89
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 5 cut(s) 434, 446, 554, 620, 629
Tru1I TTAA 2 cut(s) 291, 390
Tru9I TTAA 2 cut(s) 291, 390
TspDTI ATGAA 1 cut(s) 429
TspGWI ACGGA 1 cut(s) 682
XapI RAATTY 3 cut(s) 124, 208, 277
XceI RCATGY 1 cut(s) 31
XspI CTAG 1 cut(s) 165
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.