RchiOBHm_Chr5g0080131

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000243
Physical Location & Seq
Reverse (-)
85950210 .. 85955080
4871 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35446

Sequence Viewer

Length: 801 bp
ATGCTCAATCTTGTAGATGTCTTAAAATTCATTATCTTCCATTGCTTGAGTGGCGGATGCTTCATTGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAAAAGTTTCTCAACTTGAACAGGAGATTGCACTTCTGAGTCAATGTAAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGAATCAAAGCTCTACATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAAACGTACCCTCTTACAGATTTGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGGCCAAAATGTGATTCACAGGGACATTAAATGTGCAAATATTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCAGACTTTGGCTTGGCAAAGACTATCAAAATGAACGATATAATTTCTTGCCGAGGAACTGCAAACTGGATGGCCCCTGAGGTTGTTGAACATGAAAATCCAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTAGGATGGTTCCATACCCTAATCTCGAATGGATGGCGGCGTTATGGAAAATTATGAAGGGGGAGCCCCCTCTGGTTCCTGATTCTCTTTCAAGGGAGGCACAAGATTTTATCCATCTATGCTTACAAGTTGAGCCAGATAATCGTCCCACTGCTGCTCAGCTTTTAAAACATCCATTTGTAAATAAGCCCCTTCCTCCCACGTCTTCTGGATCAGTATCTCCTTACAATCACCACAGGCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

266

Amino Acids

29.96

Weight (kDa)

6.82

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 18 - 245 4.6e-62 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 22 - 241 1.4e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 500
AccI GTMKAC 1 cut(s) 282
AciI CCGC 2 cut(s) 54, 590
AclWI GGATC 2 cut(s) 388, 772
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 1 cut(s) 26
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 2 cut(s) 254, 535
AfiI CCNNNNNNNGG 1 cut(s) 500
AgsI TTSAA 4 cut(s) 116, 236, 482, 645
AjiI CACGTC 1 cut(s) 756
AjnI CCWGG 1 cut(s) 493
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 5 cut(s) 196, 241, 391, 512, 715
AluI AGCT 5 cut(s) 196, 241, 391, 512, 715
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 2 cut(s) 388, 772
AoxI GGCC 3 cut(s) 322, 465, 502
ApeKI GCWGC 1 cut(s) 707
ApoI RAATTY 1 cut(s) 26
Asp700I GAANNNNTTC 1 cut(s) 103
AspS9I GGNCC 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 776
AxyI CCTNAGG 1 cut(s) 471
BanII GRGCYC 1 cut(s) 621
BbsI GAAGAC 1 cut(s) 750
BbvI GCAGC 1 cut(s) 694
BccI CCATC 4 cut(s) 457, 553, 580, 675
BceAI ACGGC 1 cut(s) 337
BciT130I CCWGG 1 cut(s) 495
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 2 cut(s) 288, 555
BglII AGATCT 1 cut(s) 294
BisI GCNGC 2 cut(s) 591, 708
BlpI GCTNAGC 1 cut(s) 711
BlsI GCNGC 2 cut(s) 592, 709
Bme1390I CCNGG 1 cut(s) 495
BmgBI CACGTC 1 cut(s) 756
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 5 cut(s) 229, 468, 564, 618, 630
BmrFI CCNGG 1 cut(s) 495
BmsI GCATC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 750
Bpu1102I GCTNAGC 1 cut(s) 711
BpuEI CTTGAG 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 769
BsaJI CCNNGG 1 cut(s) 445
Bsc4I CCNNNNNNNGG 1 cut(s) 500
Bse1I ACTGG 1 cut(s) 464
Bse21I CCTNAGG 1 cut(s) 471
Bse3DI GCAATG 2 cut(s) 40, 63
Bse8I GATNNNNATC 1 cut(s) 769
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 7 cut(s) 62, 190, 468, 536, 564, 591, 724
BseJI GATNNNNATC 1 cut(s) 769
BseLI CCNNNNNNNGG 1 cut(s) 500
BseMI GCAATG 2 cut(s) 40, 63
BseMII CTCAG 3 cut(s) 125, 462, 725
BseNI ACTGG 1 cut(s) 464
BseXI GCAGC 1 cut(s) 694
BshFI GGCC 3 cut(s) 324, 467, 504
BslFI GGGAC 2 cut(s) 356, 684
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 280
BsmFI GGGAC 2 cut(s) 356, 684
BsnI GGCC 3 cut(s) 324, 467, 504
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 4 cut(s) 91, 294, 380, 764
Bsp1720I GCTNAGC 1 cut(s) 711
BspACI CCGC 2 cut(s) 54, 590
BspANI GGCC 3 cut(s) 324, 467, 504
BspCNI CTCAG 3 cut(s) 126, 463, 724
BspLI GGNNCC 5 cut(s) 229, 468, 564, 618, 630
BspPI GGATC 2 cut(s) 388, 772
BsrDI GCAATG 2 cut(s) 40, 63
BsrI ACTGG 1 cut(s) 464
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 4 cut(s) 91, 294, 380, 764
BssNAI GTATAC 1 cut(s) 283
Bst1107I GTATAC 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 1 cut(s) 538
BstC8I GCNNGC 2 cut(s) 372, 393
BstDEI CTNAG 3 cut(s) 134, 471, 711
BstF5I GGATG 7 cut(s) 62, 190, 468, 536, 564, 591, 724
BstKTI GATC 4 cut(s) 94, 297, 383, 767
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 4 cut(s) 91, 294, 380, 764
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 2 cut(s) 275, 374
BstSCI CCNGG 1 cut(s) 493
BstV1I GCAGC 1 cut(s) 694
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 2 cut(s) 294, 380
BstYI RGATCY 2 cut(s) 294, 380
BstZ17I GTATAC 1 cut(s) 283
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 3 cut(s) 324, 467, 504
BtrI CACGTC 1 cut(s) 756
BtsCI GGATG 7 cut(s) 62, 190, 468, 536, 564, 591, 724
BtsI GCAGTG 1 cut(s) 702
BtsIMutI CAGTG 1 cut(s) 702
Cac8I GCNNGC 2 cut(s) 372, 393
Cfr13I GGNCC 1 cut(s) 466
Csp6I GTAC 2 cut(s) 253, 534
CviAII CATG 4 cut(s) 149, 272, 371, 485
CviQI GTAC 2 cut(s) 253, 534
DdeI CTNAG 3 cut(s) 134, 471, 711
DpnI GATC 4 cut(s) 93, 296, 382, 766
DpnII GATC 4 cut(s) 91, 294, 380, 764
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 1 cut(s) 322
EciI GGCGGA 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 621
Eco57I CTGAAG 1 cut(s) 329
Eco81I CCTNAGG 1 cut(s) 471
EcoRII CCWGG 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 621
FaeI CATG 4 cut(s) 152, 275, 374, 488
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 2 cut(s) 356, 684
FatI CATG 4 cut(s) 148, 271, 370, 484
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 591, 708
FokI GGATG 7 cut(s) 69, 197, 475, 543, 571, 598, 711
FriOI GRGCYC 1 cut(s) 621
Fsp4HI GCNGC 2 cut(s) 591, 708
FspBI CTAG 2 cut(s) 288, 555
GluI GCNGC 2 cut(s) 591, 708
HaeIII GGCC 3 cut(s) 324, 467, 504
Hin1II CATG 4 cut(s) 152, 275, 374, 488
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 5 cut(s) 136, 188, 334, 523, 635
HpaI GTTAAC 1 cut(s) 552
HphI GGTGA 1 cut(s) 776
Hpy166II GTNNAC 3 cut(s) 146, 283, 552
Hpy188I TCNGA 2 cut(s) 135, 309
Hpy188III TCNNGA 4 cut(s) 209, 578, 632, 762
Hpy8I GTNNAC 3 cut(s) 146, 283, 552
HpyAV CCTTC 4 cut(s) 242, 515, 604, 755
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 2 cut(s) 251, 755
HpyCH4V TGCA 7 cut(s) 128, 271, 301, 356, 370, 395, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 3 cut(s) 134, 471, 711
HpySE526I ACGT 2 cut(s) 251, 755
Hsp92II CATG 4 cut(s) 152, 275, 374, 488
Ksp22I TGATCA 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 552
Kzo9I GATC 4 cut(s) 91, 294, 380, 764
LmnI GCTCC 2 cut(s) 233, 616
Lsp1109I GCAGC 1 cut(s) 694
LweI GCATC 1 cut(s) 47
MaeI CTAG 2 cut(s) 288, 555
MaeII ACGT 2 cut(s) 251, 755
MaeIII GTNAC 1 cut(s) 217
MalI GATC 4 cut(s) 93, 296, 382, 766
MboI GATC 4 cut(s) 91, 294, 380, 764
MboII GAAGA 3 cut(s) 28, 308, 750
MflI RGATCY 2 cut(s) 294, 380
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 3 cut(s) 26, 435, 603
MlyI GAGTC 2 cut(s) 145, 532
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 6 cut(s) 267, 440, 466, 633, 643, 759
MroXI GAANNNNTTC 1 cut(s) 103
MseI TTAA 4 cut(s) 23, 348, 551, 719
MslI CAYNNNNRTG 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 512
MspR9I CCNGG 1 cut(s) 495
MvaI CCWGG 1 cut(s) 495
MwoI GCNNNNNNNGC 1 cut(s) 51
NdeII GATC 4 cut(s) 91, 294, 380, 764
NlaIII CATG 4 cut(s) 152, 275, 374, 488
NlaIV GGNNCC 5 cut(s) 229, 468, 564, 618, 630
NmeAIII GCCGAG 1 cut(s) 470
NspI RCATGY 2 cut(s) 275, 374
PaeI GCATGC 1 cut(s) 374
PdmI GAANNNNTTC 1 cut(s) 103
PfeI GAWTC 3 cut(s) 188, 334, 635
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 2 cut(s) 592, 709
PleI GAGTC 2 cut(s) 144, 531
PpsI GAGTC 2 cut(s) 144, 531
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 5 cut(s) 229, 468, 564, 618, 630
PspPI GGNCC 1 cut(s) 466
PsuI RGATCY 2 cut(s) 294, 380
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 2 cut(s) 254, 535
RsaNI GTAC 2 cut(s) 253, 534
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 4 cut(s) 23, 348, 551, 719
SatI GCNGC 2 cut(s) 591, 708
Sau3AI GATC 4 cut(s) 91, 294, 380, 764
Sau96I GGNCC 1 cut(s) 466
SchI GAGTC 2 cut(s) 145, 532
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 1 cut(s) 621
SfaNI GCATC 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
SphI GCATGC 1 cut(s) 374
Sse9I AATT 3 cut(s) 26, 435, 603
SsiI CCGC 2 cut(s) 54, 590
SspI AATATT 1 cut(s) 361
SspMI CTAG 2 cut(s) 288, 555
StyD4I CCNGG 1 cut(s) 493
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 2 cut(s) 254, 758
TaqI TCGA 1 cut(s) 579
TasI AATT 3 cut(s) 26, 435, 603
TatI WGTACW 1 cut(s) 533
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 3 cut(s) 188, 334, 635
Tru1I TTAA 4 cut(s) 23, 348, 551, 719
Tru9I TTAA 4 cut(s) 23, 348, 551, 719
TscAI CASTG 1 cut(s) 709
TseI GCWGC 1 cut(s) 707
TspDTI ATGAA 6 cut(s) 19, 52, 201, 440, 501, 623
TspRI CASTG 1 cut(s) 709
Van91I CCANNNNNTGG 1 cut(s) 500
XapI RAATTY 1 cut(s) 26
XceI RCATGY 2 cut(s) 275, 374
XcmI CCANNNNNNNNNTGG 2 cut(s) 47, 516
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 103
XspI CTAG 2 cut(s) 288, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.