MD08G1173000.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000243
Physical Location & Seq
Reverse (-)
20810619 .. 20816528
5910 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1173000.v1.1.491

Sequence Viewer

Length: 1734 bp
ATGCATCACATACCTCGATTTTTCTCTCATAGCAAGGGGACGAAAGCCATGGATCCGAAGAAGAACCGCCGGAAGCCGCGGCTCGAGCGCCGTAATGCGGCGAAGCACATTGACTACGACGCCGCCTCGATGTCATCCTCACTCGACGACTCCTCGTCGTCGGCCTCGCTCCACACGCGCTCGTTCGACTTGGACCGGACGAGCTTCCGGGTCGAGGGGAACGAGGGCGATATGGAGCGGATATACAAGACCTTAGGCCTCGGACCCGACGACTTCTCGATATCGGAGGAGGACTGGGTGGCCCGGAAGATCCGCTCGACTTCGGATCTTCTCCCCATATCCAGATTGTACGGGTTGGATAGTCTGGATAGTCCGAAACCCGACGAATTGAGGGAAGAAGTGGCTGAGTTGTCCGATAGAGTAAGGGCTATTACTGTTGCTGCAACTGAGTTGACTCGAACTGACTCGCCGGTTCCAAGCGGGGGTTATAATGCTGATGCGGCTGCTGCTGCTGCTACTTCTGTTTCAGCTGGGGTTTCTATTGGGATTAAGGGTGTGCGGCCGCCCGTGCTCAGGCCGCCGCCGTCGATGAGGGTTCCGGTGATTGACGACGGATGCTCGACTTGGGACATTCTGAGGGATTTTGCGCCAGAAGGCGCAGGAAATGCGGTGGGAAAGAGATTTGGTTCTTCTTCTGCAGCTTCCTCTTGTTCTGATGAAGAAGAAGAAGAAGAAGGAGAGGTTGGAGAGGAGGGCGCTGTGGAAGGTGAGATTAGAGAGACTGTGCCGGTCTCTGAAGGGTGTTCATTTACGACGTCGAATGATGATGATTCTTCTAGCACCACCACTGTTTCTCCGAATGAGAGAATTTCGCCGAATGGGAGGCTAAAGCTTATTATCAATCACTGGGAGAAGGGTGACCTTCTTGGTAGTGGGTCTTTTGGGTCGGTGTATGAAGCCATTTCTGATGGTGGAAGCTTCATTGCTGTGAAGGAAGTTTCATTGCTTGATAAAGGAAGTCAGGGGAAGCAAAGAGTTTATCAACTTGAACAGGAGATTGCTCTTTTGAGTCAGTTTGAACATCATAACATAGTTCAATATTACGGCACCCAAAAGGATGAATCAAACCTTTATATCTTTCTGGAGCTTGTAGCTAAAGGTTCCCTTCAGAAACTCTATCAGACGTATCATCTTACAGATTCTCATGTATCTGAATACACAAGACAGATCCTCCATGGACTGAAGTATCTTCATGACCGAAAAGTGGTTCACAGGGACATCAAATGTGCAAACATTCTGGTGCATGCAAATGGATCTGTGAAGCTTGCAGATTTTGGATTGGCAAAGACTATCCAAAATATGAATGACATTAAATCTTGCCAGGGGACTGCATTCTGGATGGCCCCAGAGGTTGTGAACCGAAAGAGCCAAGGTTATGGGCTTCCAGCTGATATATGGAGCCTTGGATGCACTGTGTTGGAGATGTTAACTGGGCGGGTTCCATACTCTAATCTTGAATGGATGCAGGCATTATTTAAGATTGGAAAGGGAGAGCCCCCTCAGGTTCCCGATTCTCTTTCAAAAGATGCACAAGATTTCATTGATCGGTGCCTACAAGTTGATCCAAACAAACGCCCCTCTGCTGCTCAGCTCTTAACTCATCCATTTCTTAAGAGACCCCTTCCCACATCTTCTGGATCAGCATCTCCATATCATCACCGGGGGCCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

578

Amino Acids

63.32

Weight (kDa)

5.7

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 304 - 557 2.5e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 306 - 552 4e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AatII GACGTC 1 cut(s) 818
AccB1I GGYRCC 2 cut(s) 1106, 1608
AccBSI CCGCTC 2 cut(s) 238, 315
AccII CGCG 2 cut(s) 79, 178
AclWI GGATC 8 cut(s) 47, 60, 304, 333, 1222, 1321, 1616, 1705
AcoI YGGCCR 1 cut(s) 560
AcsI RAATTY 1 cut(s) 867
AcuI CTGAAG 3 cut(s) 816, 1151, 1262
AcyI GRCGYC 2 cut(s) 120, 815
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 5 cut(s) 97, 214, 482, 573, 1264
AflII CTTAAG 1 cut(s) 1670
AgsI TTSAA 5 cut(s) 1049, 1079, 1097, 1517, 1581
AhdI GACNNNNNGTC 1 cut(s) 154
AjnI CCWGG 1 cut(s) 1380
AjuI GAANNNNNNNTTGG 2 cut(s) 726, 758
Alw21I GWGCWC 1 cut(s) 573
Alw26I GTCTC 3 cut(s) 773, 796, 1669
AlwI GGATC 8 cut(s) 47, 60, 304, 333, 1222, 1321, 1616, 1705
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 7 cut(s) 162, 256, 300, 560, 575, 1401, 1724
ApaI GGGCCC 1 cut(s) 1728
ApeKI GCWGC 7 cut(s) 440, 503, 506, 509, 512, 698, 1643
ApoI RAATTY 1 cut(s) 867
AspLEI GCGC 5 cut(s) 90, 180, 649, 659, 758
AspS9I GGNCC 6 cut(s) 193, 263, 301, 1402, 1724, 1725
AsuC2I CCSGG 3 cut(s) 209, 304, 1721
AsuHPI GGTGA 4 cut(s) 613, 779, 929, 1709
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 2 cut(s) 193, 263
AxyI CCTNAGG 2 cut(s) 253, 1560
BaeGI GKGCMC 1 cut(s) 1728
BaeI ACNNNNGTAYC 2 cut(s) 1229, 1262
BamHI GGATCC 1 cut(s) 52
BanI GGYRCC 2 cut(s) 1106, 1608
BanII GRGCYC 2 cut(s) 1557, 1728
Bbv12I GWGCWC 1 cut(s) 573
BbvI GCAGC 7 cut(s) 427, 490, 493, 496, 499, 710, 1630
BccI CCATC 2 cut(s) 962, 1393
BceAI ACGGC 3 cut(s) 75, 568, 1120
BciT130I CCWGG 1 cut(s) 1382
BcnI CCSGG 3 cut(s) 209, 304, 1721
BcoDI GTCTC 3 cut(s) 773, 796, 1669
BfaI CTAG 1 cut(s) 837
BfmI CTRYAG 1 cut(s) 696
BfoI RGCGCY 2 cut(s) 91, 759
BfrI CTTAAG 1 cut(s) 1670
BlpI GCTNAGC 1 cut(s) 1647
Bme1390I CCNGG 4 cut(s) 209, 304, 1382, 1721
Bme18I GGWCC 2 cut(s) 193, 263
BmeRI GACNNNNNGTC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 6 cut(s) 193, 263, 301, 1402, 1724, 1725
BmrFI CCNGG 4 cut(s) 209, 304, 1382, 1721
BmrI ACTGGG 3 cut(s) 304, 916, 1500
BmsI GCATC 7 cut(s) 13, 487, 605, 1457, 1512, 1576, 1712
BmuI ACTGGG 3 cut(s) 304, 916, 1500
BpmI CTGGAG 1 cut(s) 1163
Bpu10I CCTNAGC 1 cut(s) 572
Bpu1102I GCTNAGC 1 cut(s) 1647
BpuMI CCSGG 3 cut(s) 209, 304, 1721
BsaBI GATNNNNATC 1 cut(s) 1702
BsaHI GRCGYC 2 cut(s) 120, 815
BsaI GGTCTC 2 cut(s) 796, 1669
BsaJI CCNNGG 8 cut(s) 48, 77, 259, 1234, 1381, 1429, 1462, 1720
BsaWI WCCGGW 2 cut(s) 195, 598
BsaXI ACNNNNNCTCC 4 cut(s) 838, 868, 1215, 1245
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 214, 482, 573, 1264
Bse118I RCCGGY 2 cut(s) 469, 787
Bse1I ACTGG 3 cut(s) 299, 911, 1495
Bse21I CCTNAGG 2 cut(s) 253, 1560
Bse3DI GCAATG 2 cut(s) 981, 1001
Bse8I GATNNNNATC 1 cut(s) 1702
BseBI CCWGG 1 cut(s) 1382
BseDI CCNNGG 8 cut(s) 48, 77, 259, 1234, 1381, 1429, 1462, 1720
BseGI GGATG 7 cut(s) 134, 620, 1123, 1404, 1472, 1527, 1660
BseJI GATNNNNATC 1 cut(s) 1702
BseLI CCNNNNNNNGG 5 cut(s) 97, 214, 482, 573, 1264
BseMI GCAATG 2 cut(s) 981, 1001
BseMII CTCAG 6 cut(s) 396, 438, 586, 626, 1574, 1661
BseNI ACTGG 3 cut(s) 299, 911, 1495
BseRI GAGGAG 3 cut(s) 142, 302, 764
BseSI GKGCMC 1 cut(s) 1728
BseX3I CGGCCG 1 cut(s) 560
BseXI GCAGC 7 cut(s) 427, 490, 493, 496, 499, 710, 1630
BseYI CCCAGC 2 cut(s) 530, 1727
Bsh1236I CGCG 2 cut(s) 79, 178
Bsh1285I CGRYCG 1 cut(s) 563
BshFI GGCC 7 cut(s) 164, 258, 302, 562, 577, 1403, 1726
BshNI GGYRCC 2 cut(s) 1106, 1608
BsiEI CGRYCG 1 cut(s) 563
BsiHKAI GWGCWC 1 cut(s) 573
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 8 cut(s) 70, 196, 208, 304, 470, 599, 788, 1720
BslFI GGGAC 4 cut(s) 52, 641, 1289, 1399
BslI CCNNNNNNNGG 5 cut(s) 97, 214, 482, 573, 1264
BsmAI GTCTC 3 cut(s) 773, 796, 1669
BsmFI GGGAC 4 cut(s) 52, 641, 1289, 1399
BsmI GAATGC 1 cut(s) 1391
BsnI GGCC 7 cut(s) 164, 258, 302, 562, 577, 1403, 1726
Bso31I GGTCTC 2 cut(s) 796, 1669
BsoBI CYCGRG 1 cut(s) 83
Bsp120I GGGCCC 1 cut(s) 1724
Bsp1286I GDGCHC 3 cut(s) 573, 1557, 1728
Bsp143I GATC 8 cut(s) 52, 309, 325, 1227, 1313, 1603, 1621, 1697
Bsp1720I GCTNAGC 1 cut(s) 1647
Bsp19I CCATGG 2 cut(s) 48, 1234
BspANI GGCC 7 cut(s) 164, 258, 302, 562, 577, 1403, 1726
BspCNI CTCAG 6 cut(s) 397, 439, 585, 627, 1573, 1660
BspFNI CGCG 2 cut(s) 79, 178
BspHI TCATGA 1 cut(s) 1252
BspMAI CTGCAG 1 cut(s) 700
BspPI GGATC 8 cut(s) 47, 60, 304, 333, 1222, 1321, 1616, 1705
BspT107I GGYRCC 2 cut(s) 1106, 1608
BspTI CTTAAG 1 cut(s) 1670
BspTNI GGTCTC 2 cut(s) 796, 1669
BsrBI CCGCTC 2 cut(s) 238, 315
BsrDI GCAATG 2 cut(s) 981, 1001
BsrFI RCCGGY 2 cut(s) 469, 787
BsrI ACTGG 3 cut(s) 299, 911, 1495
BssAI RCCGGY 2 cut(s) 469, 787
BssECI CCNNGG 8 cut(s) 48, 77, 259, 1234, 1381, 1429, 1462, 1720
BssMI GATC 8 cut(s) 52, 309, 325, 1227, 1313, 1603, 1621, 1697
BssNI GRCGYC 2 cut(s) 120, 815
BssT1I CCWWGG 4 cut(s) 48, 1234, 1429, 1462
Bst2UI CCWGG 1 cut(s) 1382
Bst4CI ACNGT 4 cut(s) 436, 784, 850, 1474
BstACI GRCGYC 2 cut(s) 120, 815
BstAFI CTTAAG 1 cut(s) 1670
BstAPI GCANNNNNTGC 1 cut(s) 665
BstC8I GCNNGC 3 cut(s) 1305, 1326, 1527
BstDEI CTNAG 7 cut(s) 253, 405, 447, 572, 635, 1560, 1647
BstDSI CCRYGG 3 cut(s) 48, 77, 1234
BstEII GGTNACC 1 cut(s) 917
BstF5I GGATG 7 cut(s) 134, 620, 1123, 1404, 1472, 1527, 1660
BstFNI CGCG 2 cut(s) 79, 178
BstH2I RGCGCY 2 cut(s) 91, 759
BstHHI GCGC 5 cut(s) 90, 180, 649, 659, 758
BstKTI GATC 8 cut(s) 55, 312, 328, 1230, 1316, 1606, 1624, 1700
BstMAI GTCTC 3 cut(s) 773, 796, 1669
BstMBI GATC 8 cut(s) 52, 309, 325, 1227, 1313, 1603, 1621, 1697
BstMCI CGRYCG 1 cut(s) 563
BstNI CCWGG 1 cut(s) 1382
BstNSI RCATGY 1 cut(s) 1307
BstPI GGTNACC 1 cut(s) 917
BstSCI CCNGG 4 cut(s) 207, 302, 1380, 1719
BstSFI CTRYAG 1 cut(s) 696
BstSLI GKGCMC 1 cut(s) 1728
BstUI CGCG 2 cut(s) 79, 178
BstV1I GCAGC 7 cut(s) 427, 490, 493, 496, 499, 710, 1630
BstX2I RGATCY 5 cut(s) 52, 309, 325, 1227, 1313
BstXI CCANNNNNNTGG 1 cut(s) 1436
BstYI RGATCY 5 cut(s) 52, 309, 325, 1227, 1313
BstZI CGGCCG 1 cut(s) 560
Bsu36I CCTNAGG 2 cut(s) 253, 1560
BsuRI GGCC 7 cut(s) 164, 258, 302, 562, 577, 1403, 1726
BtgI CCRYGG 3 cut(s) 48, 77, 1234
BtsCI GGATG 7 cut(s) 134, 620, 1123, 1404, 1472, 1527, 1660
BtsIMutI CAGTG 3 cut(s) 846, 904, 1470
Cac8I GCNNGC 3 cut(s) 1305, 1326, 1527
CciI TCATGA 1 cut(s) 1252
CciNI GCGGCCGC 1 cut(s) 560
CfoI GCGC 5 cut(s) 90, 180, 649, 659, 758
Cfr10I RCCGGY 2 cut(s) 469, 787
Cfr13I GGNCC 6 cut(s) 193, 263, 301, 1402, 1724, 1725
Cfr42I CCGCGG 1 cut(s) 80
CseI GACGC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 349
CviAII CATG 5 cut(s) 49, 1205, 1235, 1253, 1304
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 7 cut(s) 253, 405, 447, 572, 635, 1560, 1647
DpnI GATC 8 cut(s) 54, 311, 327, 1229, 1315, 1605, 1623, 1699
DpnII GATC 8 cut(s) 52, 309, 325, 1227, 1313, 1603, 1621, 1697
DriI GACNNNNNGTC 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 560
EagI CGGCCG 1 cut(s) 560
Eam1105I GACNNNNNGTC 1 cut(s) 154
EclXI CGGCCG 1 cut(s) 560
Eco130I CCWWGG 4 cut(s) 48, 1234, 1429, 1462
Eco147I AGGCCT 1 cut(s) 258
Eco24I GRGCYC 2 cut(s) 1557, 1728
Eco31I GGTCTC 2 cut(s) 796, 1669
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 2 cut(s) 193, 263
Eco52I CGGCCG 1 cut(s) 560
Eco57I CTGAAG 3 cut(s) 816, 1151, 1262
Eco81I CCTNAGG 2 cut(s) 253, 1560
Eco88I CYCGRG 1 cut(s) 83
Eco91I GGTNACC 1 cut(s) 917
EcoO109I RGGNCCY 1 cut(s) 1724
EcoO65I GGTNACC 1 cut(s) 917
EcoRII CCWGG 1 cut(s) 1380
EcoRV GATATC 1 cut(s) 282
EcoT14I CCWWGG 4 cut(s) 48, 1234, 1429, 1462
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 1557, 1728
ErhI CCWWGG 4 cut(s) 48, 1234, 1429, 1462
FaeI CATG 5 cut(s) 52, 1208, 1238, 1256, 1307
FalI AAGNNNNNCTT 2 cut(s) 1149, 1181
FaqI GGGAC 4 cut(s) 52, 641, 1289, 1399
FatI CATG 5 cut(s) 48, 1204, 1234, 1252, 1303
FauI CCCGC 2 cut(s) 473, 1488
FokI GGATG 7 cut(s) 121, 627, 1130, 1411, 1479, 1534, 1647
FriOI GRGCYC 2 cut(s) 1557, 1728
FspBI CTAG 1 cut(s) 837
GlaI GCGC 5 cut(s) 89, 179, 648, 658, 757
GsaI CCCAGC 2 cut(s) 534, 1731
GsuI CTGGAG 1 cut(s) 1163
HaeII RGCGCY 2 cut(s) 91, 759
HaeIII GGCC 7 cut(s) 164, 258, 302, 562, 577, 1403, 1726
HapII CCGG 8 cut(s) 70, 196, 208, 304, 470, 599, 788, 1720
HgaI GACGC 1 cut(s) 128
HhaI GCGC 5 cut(s) 90, 180, 649, 659, 758
Hin1I GRCGYC 2 cut(s) 120, 815
Hin1II CATG 5 cut(s) 52, 1208, 1238, 1256, 1307
Hin6I GCGC 5 cut(s) 88, 178, 647, 657, 756
HinP1I GCGC 5 cut(s) 88, 178, 647, 657, 756
HincII GTYRAC 2 cut(s) 453, 1488
HindII GTYRAC 2 cut(s) 453, 1488
HindIII AAGCTT 3 cut(s) 890, 976, 1322
HinfI GANTC 8 cut(s) 149, 454, 464, 830, 1069, 1121, 1199, 1571
HpaI GTTAAC 1 cut(s) 1488
HpaII CCGG 8 cut(s) 70, 196, 208, 304, 470, 599, 788, 1720
HphI GGTGA 4 cut(s) 613, 779, 929, 1709
Hpy166II GTNNAC 4 cut(s) 453, 1270, 1417, 1488
Hpy188III TCNNGA 9 cut(s) 277, 342, 365, 1142, 1253, 1396, 1514, 1568, 1695
Hpy8I GTNNAC 4 cut(s) 453, 1270, 1417, 1488
HpyAV CCTTC 9 cut(s) 647, 728, 758, 791, 907, 932, 985, 1175, 1691
HpyCH4III ACNGT 4 cut(s) 436, 784, 850, 1474
HpyCH4IV ACGT 2 cut(s) 815, 1184
HpyF3I CTNAG 7 cut(s) 253, 405, 447, 572, 635, 1560, 1647
HpySE526I ACGT 2 cut(s) 815, 1184
Hsp92I GRCGYC 2 cut(s) 120, 815
Hsp92II CATG 5 cut(s) 52, 1208, 1238, 1256, 1307
HspAI GCGC 5 cut(s) 88, 178, 647, 657, 756
KspAI GTTAAC 1 cut(s) 1488
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 8 cut(s) 52, 309, 325, 1227, 1313, 1603, 1621, 1697
LmnI GCTCC 4 cut(s) 174, 235, 1144, 1458
Lsp1109I GCAGC 7 cut(s) 427, 490, 493, 496, 499, 710, 1630
LweI GCATC 7 cut(s) 13, 487, 605, 1457, 1512, 1576, 1712
MaeI CTAG 1 cut(s) 837
MaeII ACGT 2 cut(s) 815, 1184
MaeIII GTNAC 1 cut(s) 917
MalI GATC 8 cut(s) 54, 311, 327, 1229, 1315, 1605, 1623, 1699
MbiI CCGCTC 2 cut(s) 238, 315
MboI GATC 8 cut(s) 52, 309, 325, 1227, 1313, 1603, 1621, 1697
MflI RGATCY 5 cut(s) 52, 309, 325, 1227, 1313
MhlI GDGCHC 3 cut(s) 573, 1557, 1728
MluCI AATT 2 cut(s) 386, 867
MlyI GAGTC 4 cut(s) 143, 448, 458, 1078
MmeI TCCRAC 3 cut(s) 336, 724, 1458
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 6 cut(s) 549, 1371, 1487, 1536, 1655, 1671
MslI CAYNNNNRTG 3 cut(s) 986, 1298, 1308
MspA1I CMGCKG 4 cut(s) 79, 530, 1448, 1731
MspCI CTTAAG 1 cut(s) 1670
MspI CCGG 8 cut(s) 70, 196, 208, 304, 470, 599, 788, 1720
MspR9I CCNGG 4 cut(s) 209, 304, 1382, 1721
Mva1269I GAATGC 1 cut(s) 1391
MvaI CCWGG 1 cut(s) 1382
MvnI CGCG 2 cut(s) 79, 178
NciI CCSGG 3 cut(s) 209, 304, 1721
NcoI CCATGG 2 cut(s) 48, 1234
NdeII GATC 8 cut(s) 52, 309, 325, 1227, 1313, 1603, 1621, 1697
NlaIII CATG 5 cut(s) 52, 1208, 1238, 1256, 1307
NmuCI GTSAC 1 cut(s) 917
NotI GCGGCCGC 1 cut(s) 560
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 1307
PaeI GCATGC 1 cut(s) 1307
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 1252
PceI AGGCCT 1 cut(s) 258
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 267
PctI GAATGC 1 cut(s) 1391
PfeI GAWTC 4 cut(s) 830, 1121, 1199, 1571
PleI GAGTC 4 cut(s) 143, 448, 458, 1077
PpsI GAGTC 4 cut(s) 143, 448, 458, 1077
PsiI TTATAA 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 1380
PspEI GGTNACC 1 cut(s) 917
PspFI CCCAGC 2 cut(s) 530, 1727
PspGI CCWGG 1 cut(s) 1380
PspOMI GGGCCC 1 cut(s) 1724
PspPI GGNCC 6 cut(s) 193, 263, 301, 1402, 1724, 1725
PspXI VCTCGAGB 1 cut(s) 83
PstI CTGCAG 1 cut(s) 700
PsuI RGATCY 5 cut(s) 52, 309, 325, 1227, 1313
PvuII CAGCTG 3 cut(s) 530, 1448, 1731
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 3 cut(s) 986, 1298, 1308
SacII CCGCGG 1 cut(s) 80
SaqAI TTAA 6 cut(s) 549, 1371, 1487, 1536, 1655, 1671
Sau3AI GATC 8 cut(s) 52, 309, 325, 1227, 1313, 1603, 1621, 1697
Sau96I GGNCC 6 cut(s) 193, 263, 301, 1402, 1724, 1725
SchI GAGTC 4 cut(s) 143, 448, 458, 1078
ScrFI CCNGG 4 cut(s) 209, 304, 1382, 1721
SduI GDGCHC 3 cut(s) 573, 1557, 1728
SfaNI GCATC 7 cut(s) 13, 487, 605, 1457, 1512, 1576, 1712
SfcI CTRYAG 1 cut(s) 696
Sfr274I CTCGAG 1 cut(s) 83
Sfr303I CCGCGG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 2 cut(s) 193, 263
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 3 cut(s) 986, 1298, 1308
SmlI CTYRAG 2 cut(s) 83, 1670
SmoI CTYRAG 2 cut(s) 83, 1670
SphI GCATGC 1 cut(s) 1307
Sse9I AATT 2 cut(s) 386, 867
SseBI AGGCCT 1 cut(s) 258
SspI AATATT 1 cut(s) 1100
SspMI CTAG 1 cut(s) 837
StuI AGGCCT 1 cut(s) 258
StyD4I CCNGG 4 cut(s) 207, 302, 1380, 1719
StyI CCWWGG 4 cut(s) 48, 1234, 1429, 1462
TaaI ACNGT 4 cut(s) 436, 784, 850, 1474
TaiI ACGT 2 cut(s) 818, 1187
TaqII GACCGA 1 cut(s) 1272
TasI AATT 2 cut(s) 386, 867
TauI GCSGC 9 cut(s) 79, 82, 101, 125, 503, 562, 565, 580, 583
TfiI GAWTC 4 cut(s) 830, 1121, 1199, 1571
Tru1I TTAA 6 cut(s) 549, 1371, 1487, 1536, 1655, 1671
Tru9I TTAA 6 cut(s) 549, 1371, 1487, 1536, 1655, 1671
TscAI CASTG 3 cut(s) 853, 911, 1477
TseFI GTSAC 1 cut(s) 917
TseI GCWGC 7 cut(s) 440, 503, 506, 509, 512, 698, 1643
Tsp45I GTSAC 1 cut(s) 917
TspDTI ATGAA 9 cut(s) 732, 795, 969, 970, 990, 1134, 1241, 1376, 1588
TspGWI ACGGA 1 cut(s) 627
TspRI CASTG 3 cut(s) 853, 911, 1477
Vha464I CTTAAG 1 cut(s) 1670
VpaK11BI GGWCC 2 cut(s) 193, 263
XapI RAATTY 1 cut(s) 867
XceI RCATGY 1 cut(s) 1307
XcmI CCANNNNNNNNNTGG 1 cut(s) 1452
XhoI CTCGAG 1 cut(s) 83
XspI CTAG 1 cut(s) 837
ZraI GACGTC 1 cut(s) 816
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.