Rh5AG516900

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000243
Physical Location & Seq
Reverse (-)
87749117 .. 87754523
5407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG516900.1

Sequence Viewer

Length: 1422 bp
ATGTGGGATCTGAATGAGCCTCCTAATCAGGGACGCGTGGAGGACGACGGCACCACCTCGGCCGACGGCAACGGAAAAAGTGGCTGCAAGATATTCGGGGTCTGGTTCACGGACATTGCTGATTCGCAACCCGTGACCCGACAGTTCTTCCCGGTAGATGTATCCTCCGAAATGGAACTCCCGCCTGGAGGTGGTGTTGCCGCCGGGCCGGTTGCTTCTTCCTCTTCTTCATCATCACCTGTTCAGTGGAAGTATGATTCACCTGCTCAGTGGAATTGCAGCGCAGGTGTCGGAATTAAGGGAGCACGGCTGCTGGGGCTGAAGCCGCCGCCGTCGATGGTGAGGGTTCCAGTGATTGACAATGGATGCTCGACTTGGGACATTTTGAGGGACTTTGCGCCGGAAGAAGAGAGGGAATCGTTGGAAATGGTGCAGAGAAGGTTTAGTTCCTTTTCCTCGTCTTCTTCTGATGAAGAAGAAGAAGTTGAAGAAGGAGAGGATGTTGACTTTTCCAGCACTACCGCAGAGATTTCCAGTATTTCTCCAAATGGAAGGTTTTCGCCGAATGGGAAGCCTAAGCTTACTGTCACTAATTGGGATAAGGGTGATTTGCTCGGCAGCGGCTCTTTCGGGTCTGTGTATGAAGGAATTTCTGATGGCGGATGCTTCATTGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGGAGGCAAAGAGTTTCTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGGATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAAACGTACACTCTTACTGATTCAATTGTTTCTGCATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCGAAAAGTGATGCACAGGGACATTAAATGTGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCTGACTTTGGATTGGCAAAGACTATCAAAATGAACGATATAAAATCTTGCCAAGGAACTGCATACTGGATGGCCCCTGAGGTTGTTAATCGTAAGAGTCAAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGCTAACTGGGATGGTTCCATACAATAACCTCGAATGGATTCGGGCATTATGGAAAATTGGAAAAGGGGAGCCCCCTACGGTTCCTGATTCTCTTTCAAGAGAGGCACAAGATTTCATCCATCTATGCTTACAAGTTAAGCCAGATAATCGTCCCACTGCTGCTGAGCTTTTAAAACATCCATTTGTAAATAAGCCCCTTCCTCCCACGTCTTCTGGATCAGTATCTCCTTACAATCACCACAGGCAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

473

Amino Acids

51.88

Weight (kDa)

5.22

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 201 - 452 1.7e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 203 - 448 1.4e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 271, 275
Acc36I ACCTGC 2 cut(s) 271, 275
AccB1I GGYRCC 1 cut(s) 50
AccII CGCG 1 cut(s) 36
AciI CCGC 7 cut(s) 182, 201, 326, 329, 522, 621, 660
AclWI GGATC 4 cut(s) 15, 817, 1009, 1393
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 1 cut(s) 648
AcuI CTGAAG 2 cut(s) 341, 950
AfaI GTAC 2 cut(s) 875, 1156
AfiI CCNNNNNNNGG 4 cut(s) 29, 188, 191, 1414
AflIII ACRYGT 1 cut(s) 34
AgsI TTSAA 6 cut(s) 488, 737, 767, 857, 891, 1266
AjiI CACGTC 1 cut(s) 1377
AjnI CCWGG 2 cut(s) 184, 708
AluBI AGCT 6 cut(s) 580, 817, 862, 1012, 1133, 1336
AluI AGCT 6 cut(s) 580, 817, 862, 1012, 1133, 1336
Alw21I GWGCWC 1 cut(s) 307
AlwI GGATC 4 cut(s) 15, 817, 1009, 1393
AoxI GGCC 4 cut(s) 60, 206, 1086, 1123
ApeKI GCWGC 5 cut(s) 84, 279, 310, 618, 1328
ApoI RAATTY 1 cut(s) 648
Asp700I GAANNNNTTC 2 cut(s) 556, 1206
AspLEI GCGC 2 cut(s) 284, 400
AspS9I GGNCC 2 cut(s) 206, 1087
AsuC2I CCSGG 2 cut(s) 152, 205
AsuHPI GGTGA 5 cut(s) 228, 252, 352, 617, 1397
AxyI CCTNAGG 1 cut(s) 1092
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 1 cut(s) 1242
BbsI GAAGAC 2 cut(s) 453, 1371
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 5 cut(s) 71, 291, 297, 630, 1315
BccI CCATC 6 cut(s) 331, 650, 800, 1078, 1174, 1296
BceAI ACGGC 4 cut(s) 64, 82, 316, 323
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 2 cut(s) 186, 710
BciVI GTATCC 1 cut(s) 172
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 2 cut(s) 152, 205
BfaI CTAG 1 cut(s) 909
BfuAI ACCTGC 2 cut(s) 271, 275
BfuI GTATCC 1 cut(s) 172
BglII AGATCT 1 cut(s) 915
BisI GCNGC 9 cut(s) 85, 201, 280, 311, 326, 329, 619, 622, 1329
BlpI GCTNAGC 1 cut(s) 1332
BlsI GCNGC 9 cut(s) 86, 202, 281, 312, 327, 330, 620, 623, 1330
Bme1390I CCNGG 4 cut(s) 152, 186, 205, 710
BmgBI CACGTC 1 cut(s) 1377
BmgT120I GGNCC 2 cut(s) 206, 1087
BmiI GGNNCC 7 cut(s) 52, 348, 850, 1089, 1185, 1239, 1251
BmrFI CCNGG 4 cut(s) 152, 186, 205, 710
BmrI ACTGGG 1 cut(s) 1185
BmsI GCATC 4 cut(s) 356, 653, 945, 1158
BmuI ACTGGG 1 cut(s) 1185
BpiI GAAGAC 2 cut(s) 453, 1371
BpmI CTGGAG 1 cut(s) 207
Bpu10I CCTNAGC 1 cut(s) 576
Bpu1102I GCTNAGC 1 cut(s) 1332
BpuMI CCSGG 2 cut(s) 152, 205
BsaBI GATNNNNATC 1 cut(s) 1390
BsaJI CCNNGG 3 cut(s) 57, 709, 1066
BsaXI ACNNNNNCTCC 4 cut(s) 180, 210, 486, 516
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 188, 191, 1414
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 4 cut(s) 350, 534, 1085, 1180
Bse21I CCTNAGG 1 cut(s) 1092
Bse3DI GCAATG 2 cut(s) 114, 669
Bse8I GATNNNNATC 1 cut(s) 1390
BseBI CCWGG 2 cut(s) 186, 710
BseDI CCNNGG 3 cut(s) 57, 709, 1066
BseGI GGATG 9 cut(s) 371, 505, 668, 811, 1089, 1157, 1185, 1284, 1345
BseJI GATNNNNATC 1 cut(s) 1390
BseLI CCNNNNNNNGG 4 cut(s) 29, 188, 191, 1414
BseMI GCAATG 2 cut(s) 114, 669
BseMII CTCAG 4 cut(s) 281, 746, 1083, 1323
BseNI ACTGG 4 cut(s) 350, 534, 1085, 1180
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 5 cut(s) 71, 291, 297, 630, 1315
BseYI CCCAGC 1 cut(s) 313
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 1 cut(s) 36
Bsh1285I CGRYCG 2 cut(s) 63, 946
BshFI GGCC 4 cut(s) 62, 208, 1088, 1125
BshNI GGYRCC 1 cut(s) 50
BsiEI CGRYCG 2 cut(s) 63, 946
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 4 cut(s) 152, 204, 209, 401
BslFI GGGAC 5 cut(s) 45, 392, 404, 977, 1305
BslI CCNNNNNNNGG 4 cut(s) 29, 188, 191, 1414
BsmFI GGGAC 5 cut(s) 45, 392, 404, 977, 1305
BsnI GGCC 4 cut(s) 62, 208, 1088, 1125
Bsp1286I GDGCHC 2 cut(s) 307, 1242
Bsp143I GATC 6 cut(s) 7, 697, 809, 915, 1001, 1385
Bsp1720I GCTNAGC 1 cut(s) 1332
BspACI CCGC 7 cut(s) 182, 201, 326, 329, 522, 621, 660
BspANI GGCC 4 cut(s) 62, 208, 1088, 1125
BspCNI CTCAG 4 cut(s) 280, 747, 1084, 1324
BspFNI CGCG 1 cut(s) 36
BspLI GGNNCC 7 cut(s) 52, 348, 850, 1089, 1185, 1239, 1251
BspMI ACCTGC 2 cut(s) 271, 275
BspPI GGATC 4 cut(s) 15, 817, 1009, 1393
BspQI GCTCTTC 1 cut(s) 756
BspT107I GGYRCC 1 cut(s) 50
BsrDI GCAATG 2 cut(s) 114, 669
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 4 cut(s) 350, 534, 1085, 1180
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 3 cut(s) 57, 709, 1066
BssMI GATC 6 cut(s) 7, 697, 809, 915, 1001, 1385
BssT1I CCWWGG 1 cut(s) 1066
Bst2UI CCWGG 2 cut(s) 186, 710
Bst4CI ACNGT 4 cut(s) 144, 586, 1159, 1249
Bst6I CTCTTC 3 cut(s) 229, 402, 756
BstC8I GCNNGC 2 cut(s) 993, 1014
BstDEI CTNAG 5 cut(s) 267, 576, 755, 1092, 1332
BstF5I GGATG 9 cut(s) 371, 505, 668, 811, 1089, 1157, 1185, 1284, 1345
BstFNI CGCG 1 cut(s) 36
BstHHI GCGC 2 cut(s) 284, 400
BstKTI GATC 6 cut(s) 10, 700, 812, 918, 1004, 1388
BstMBI GATC 6 cut(s) 7, 697, 809, 915, 1001, 1385
BstMCI CGRYCG 2 cut(s) 63, 946
BstMWI GCNNNNNNNGC 2 cut(s) 316, 325
BstNI CCWGG 2 cut(s) 186, 710
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 4 cut(s) 150, 184, 203, 708
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 5 cut(s) 71, 291, 297, 630, 1315
BstV2I GAAGAC 2 cut(s) 453, 1371
BstX2I RGATCY 3 cut(s) 7, 915, 1001
BstYI RGATCY 3 cut(s) 7, 915, 1001
BstZI CGGCCG 1 cut(s) 60
Bsu36I CCTNAGG 1 cut(s) 1092
BsuI GTATCC 1 cut(s) 172
BsuRI GGCC 4 cut(s) 62, 208, 1088, 1125
BtrI CACGTC 1 cut(s) 1377
BtsCI GGATG 9 cut(s) 371, 505, 668, 811, 1089, 1157, 1185, 1284, 1345
BtsI GCAGTG 1 cut(s) 1323
BtsIMutI CAGTG 4 cut(s) 251, 275, 357, 1323
BveI ACCTGC 2 cut(s) 271, 275
Cac8I GCNNGC 2 cut(s) 993, 1014
CfoI GCGC 2 cut(s) 284, 400
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 206, 1087
CseI GACGC 1 cut(s) 42
Csp6I GTAC 2 cut(s) 874, 1155
CviAII CATG 2 cut(s) 770, 992
CviQI GTAC 2 cut(s) 874, 1155
DdeI CTNAG 5 cut(s) 267, 576, 755, 1092, 1332
DpnI GATC 6 cut(s) 9, 699, 811, 917, 1003, 1387
DpnII GATC 6 cut(s) 7, 697, 809, 915, 1001, 1385
DraI TTTAAA 1 cut(s) 1341
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 3 cut(s) 229, 402, 756
EarI CTCTTC 3 cut(s) 229, 402, 756
EciI GGCGGA 1 cut(s) 675
EclXI CGGCCG 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 1066
Eco24I GRGCYC 1 cut(s) 1242
Eco52I CGGCCG 1 cut(s) 60
Eco57I CTGAAG 2 cut(s) 341, 950
Eco81I CCTNAGG 1 cut(s) 1092
EcoRII CCWGG 2 cut(s) 184, 708
EcoT14I CCWWGG 1 cut(s) 1066
EcoT38I GRGCYC 1 cut(s) 1242
ErhI CCWWGG 1 cut(s) 1066
FaeI CATG 2 cut(s) 773, 995
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 5 cut(s) 45, 392, 404, 977, 1305
FatI CATG 2 cut(s) 769, 991
FauI CCCGC 1 cut(s) 189
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 9 cut(s) 85, 201, 280, 311, 326, 329, 619, 622, 1329
FokI GGATG 9 cut(s) 378, 512, 675, 818, 1096, 1164, 1192, 1271, 1332
FriOI GRGCYC 1 cut(s) 1242
Fsp4HI GCNGC 9 cut(s) 85, 201, 280, 311, 326, 329, 619, 622, 1329
FspBI CTAG 1 cut(s) 909
GlaI GCGC 2 cut(s) 283, 399
GluI GCNGC 9 cut(s) 85, 201, 280, 311, 326, 329, 619, 622, 1329
GsaI CCCAGC 1 cut(s) 317
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 4 cut(s) 62, 208, 1088, 1125
HapII CCGG 4 cut(s) 152, 204, 209, 401
HgaI GACGC 1 cut(s) 42
HhaI GCGC 2 cut(s) 284, 400
Hin1II CATG 2 cut(s) 773, 995
Hin6I GCGC 2 cut(s) 282, 398
HinP1I GCGC 2 cut(s) 282, 398
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 2 cut(s) 578, 1010
HinfI GANTC 9 cut(s) 122, 257, 416, 757, 887, 1111, 1144, 1207, 1256
HpaII CCGG 4 cut(s) 152, 204, 209, 401
HphI GGTGA 5 cut(s) 228, 252, 352, 617, 1397
Hpy166II GTNNAC 3 cut(s) 108, 505, 876
Hpy188I TCNGA 7 cut(s) 12, 169, 293, 469, 655, 756, 930
Hpy188III TCNNGA 5 cut(s) 830, 941, 1253, 1266, 1383
Hpy8I GTNNAC 3 cut(s) 108, 505, 876
Hpy99I CGWCG 3 cut(s) 50, 68, 337
HpyAV CCTTC 7 cut(s) 432, 485, 546, 638, 863, 1136, 1376
HpyCH4III ACNGT 4 cut(s) 144, 586, 1159, 1249
HpyCH4IV ACGT 2 cut(s) 872, 1376
HpyCH4V TGCA 9 cut(s) 87, 279, 433, 902, 922, 958, 977, 991, 1076
HpyF10VI GCNNNNNNNGC 2 cut(s) 316, 325
HpyF3I CTNAG 5 cut(s) 267, 576, 755, 1092, 1332
HpySE526I ACGT 2 cut(s) 872, 1376
Hsp92II CATG 2 cut(s) 773, 995
HspAI GCGC 2 cut(s) 282, 398
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 6 cut(s) 7, 697, 809, 915, 1001, 1385
LguI GCTCTTC 1 cut(s) 756
LmnI GCTCC 3 cut(s) 302, 854, 1237
Lsp1109I GCAGC 5 cut(s) 71, 291, 297, 630, 1315
LweI GCATC 4 cut(s) 356, 653, 945, 1158
MaeI CTAG 1 cut(s) 909
MaeII ACGT 2 cut(s) 872, 1376
MaeIII GTNAC 3 cut(s) 133, 586, 838
MalI GATC 6 cut(s) 9, 699, 811, 917, 1003, 1387
MboI GATC 6 cut(s) 7, 697, 809, 915, 1001, 1385
MfeI CAATTG 1 cut(s) 891
MflI RGATCY 3 cut(s) 7, 915, 1001
MhlI GDGCHC 2 cut(s) 307, 1242
MluCI AATT 6 cut(s) 274, 294, 592, 648, 891, 1224
MluI ACGCGT 1 cut(s) 34
MlyI GAGTC 3 cut(s) 766, 1120, 1153
MmeI TCCRAC 3 cut(s) 271, 402, 1143
MroXI GAANNNNTTC 2 cut(s) 556, 1206
MseI TTAA 6 cut(s) 297, 969, 1101, 1305, 1340, 1420
MslI CAYNNNNRTG 1 cut(s) 996
MspA1I CMGCKG 2 cut(s) 621, 1133
MspI CCGG 4 cut(s) 152, 204, 209, 401
MspR9I CCNGG 4 cut(s) 152, 186, 205, 710
MunI CAATTG 1 cut(s) 891
MvaI CCWGG 2 cut(s) 186, 710
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 2 cut(s) 316, 325
NciI CCSGG 2 cut(s) 152, 205
NdeII GATC 6 cut(s) 7, 697, 809, 915, 1001, 1385
NlaIII CATG 2 cut(s) 773, 995
NlaIV GGNNCC 7 cut(s) 52, 348, 850, 1089, 1185, 1239, 1251
NmeAIII GCCGAG 2 cut(s) 38, 594
NmuCI GTSAC 2 cut(s) 133, 586
NspI RCATGY 1 cut(s) 995
PaeI GCATGC 1 cut(s) 995
PaqCI CACCTGC 2 cut(s) 271, 275
PciSI GCTCTTC 1 cut(s) 756
PdmI GAANNNNTTC 2 cut(s) 556, 1206
PfeI GAWTC 6 cut(s) 122, 257, 416, 887, 1207, 1256
PkrI GCNGC 9 cut(s) 86, 202, 281, 312, 327, 330, 620, 623, 1330
PleI GAGTC 3 cut(s) 765, 1119, 1152
PpsI GAGTC 3 cut(s) 765, 1119, 1152
Psp6I CCWGG 2 cut(s) 184, 708
PspFI CCCAGC 1 cut(s) 313
PspGI CCWGG 2 cut(s) 184, 708
PspN4I GGNNCC 7 cut(s) 52, 348, 850, 1089, 1185, 1239, 1251
PspPI GGNCC 2 cut(s) 206, 1087
PsuI RGATCY 3 cut(s) 7, 915, 1001
PvuII CAGCTG 1 cut(s) 1133
RsaI GTAC 2 cut(s) 875, 1156
RsaNI GTAC 2 cut(s) 874, 1155
RseI CAYNNNNRTG 1 cut(s) 996
SapI GCTCTTC 1 cut(s) 756
SaqAI TTAA 6 cut(s) 297, 969, 1101, 1305, 1340, 1420
SatI GCNGC 9 cut(s) 85, 201, 280, 311, 326, 329, 619, 622, 1329
Sau3AI GATC 6 cut(s) 7, 697, 809, 915, 1001, 1385
Sau96I GGNCC 2 cut(s) 206, 1087
SchI GAGTC 3 cut(s) 766, 1120, 1153
ScrFI CCNGG 4 cut(s) 152, 186, 205, 710
SduI GDGCHC 2 cut(s) 307, 1242
SfaNI GCATC 4 cut(s) 356, 653, 945, 1158
SmiMI CAYNNNNRTG 1 cut(s) 996
SphI GCATGC 1 cut(s) 995
Sse9I AATT 6 cut(s) 274, 294, 592, 648, 891, 1224
SsiI CCGC 7 cut(s) 182, 201, 326, 329, 522, 621, 660
SspMI CTAG 1 cut(s) 909
StyD4I CCNGG 4 cut(s) 150, 184, 203, 708
StyI CCWWGG 1 cut(s) 1066
TaaI ACNGT 4 cut(s) 144, 586, 1159, 1249
TaiI ACGT 2 cut(s) 875, 1379
TaqI TCGA 3 cut(s) 335, 371, 1200
TaqII GACCGA 1 cut(s) 960
TasI AATT 6 cut(s) 274, 294, 592, 648, 891, 1224
TatI WGTACW 1 cut(s) 1154
TauI GCSGC 4 cut(s) 203, 328, 331, 624
TfiI GAWTC 6 cut(s) 122, 257, 416, 887, 1207, 1256
Tru1I TTAA 6 cut(s) 297, 969, 1101, 1305, 1340, 1420
Tru9I TTAA 6 cut(s) 297, 969, 1101, 1305, 1340, 1420
TscAI CASTG 4 cut(s) 251, 275, 357, 1330
TseFI GTSAC 2 cut(s) 133, 586
TseI GCWGC 5 cut(s) 84, 279, 310, 618, 1328
Tsp45I GTSAC 2 cut(s) 133, 586
TspDTI ATGAA 6 cut(s) 219, 486, 657, 658, 1061, 1273
TspGWI ACGGA 2 cut(s) 87, 125
TspRI CASTG 4 cut(s) 251, 275, 357, 1330
XapI RAATTY 1 cut(s) 648
XceI RCATGY 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 1137
XmnI GAANNNNTTC 2 cut(s) 556, 1206
XspI CTAG 1 cut(s) 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.