Rroxscaffold_7G00173210

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000243
Physical Location & Seq
Forward (+)
13306564 .. 13310030
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173210.1

Sequence Viewer

Length: 972 bp
ATGTACCAAGTAGAGACGAAATTGAATGCATTCCCAGCACCAAAAAGAAGAAAGACCAAATTGGGTGCAGTGGATATTTCTCCAAAAACCTGGACAAAGGGTCGGCTTATTGGTCGTGGCTCTTTCGGCTCGGTCTATCAAGGATCTTCTTCAGATGGATTTGTTTTCGCTGCCAAGGAAGTTTCATTGCTTGATCCAGGAAGTCCGGGGAGGAGCTGTCTTTCTCAACTTCAACAGGAGATTGAGTTTCTTAGTTGGCTTGAACACAAGAACATAGTTAAATACTATGGCTCATTTGAGTATGGAACAAATCTTTATATGTGTATGGAGCATGCACCTAATGGATCCATTCGACAACTCTATCAGAGATCGTATCTTTCATATTCTCGTGTCTCTAAATACACAAAAGAGATTCTTCTCGGTCTAAAGTATCTCCATGACCATAATGTGGTTCACAGGGATATCAAATGTGCAAATATTTTCGTGGATGCTTATGGATCTGCGAAGCTTGCAGATTTTGGATTGGCAAAGATAATCACCAAAATGAATGAAATTCAATCTTTGCAGGGGACTGCATTCTGGATGGCCCCTGAGGTTTTTACTGCAAAAATGAAGAACCAAGGTTATGGGCCTCCAGCAGATATATGGAGCCTTGGTTGCACTGTGTTGGAGATGTTAACAAGGCAGGTTCCGTACCCTGGTTTGGAACCGTTTCAGGCATTTTTTAAGATTGCAATGGGGGAGCTCCCTCACGTTCCTCATTTTCTTTGTAAGGAGGCGCGAGATTTCATCCATCAGTGTCTACAAGTTAATCCAAACAATCGACACACTGCTGCTCAGCTCTTATGTCATCCATTTGTCACGTCTGGGCCTATCAAACTACCCCATGTGTTCGATGGTCTTTGTATGATGAAGGGAAATGAACTGTATGCAAGAGGATATTTTGATGGGACTATTAGTCATTTAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

323

Amino Acids

36.44

Weight (kDa)

8.96

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 32 - 287 2.6e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 34 - 282 1.9e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 676
AccB7I CCANNNNNTGG 1 cut(s) 448
AccI GTMKAC 1 cut(s) 802
AccII CGCG 1 cut(s) 781
AclWI GGATC 5 cut(s) 151, 188, 339, 352, 505
AcsI RAATTY 1 cut(s) 552
AcuI CTGAAG 1 cut(s) 135
AfaI GTAC 2 cut(s) 5, 695
AfiI CCNNNNNNNGG 3 cut(s) 448, 698, 703
AgsI TTSAA 4 cut(s) 25, 233, 263, 557
AhdI GACNNNNNGTC 2 cut(s) 99, 957
AjiI CACGTC 1 cut(s) 864
AjnI CCWGG 3 cut(s) 89, 196, 697
AluBI AGCT 4 cut(s) 216, 508, 745, 841
AluI AGCT 4 cut(s) 216, 508, 745, 841
Alw21I GWGCWC 1 cut(s) 747
Alw26I GTCTC 2 cut(s) 8, 397
AlwI GGATC 5 cut(s) 151, 188, 339, 352, 505
AoxI GGCC 3 cut(s) 585, 629, 869
ApeKI GCWGC 2 cut(s) 170, 833
ApoI RAATTY 1 cut(s) 552
Asp700I GAANNNNTTC 2 cut(s) 29, 711
AspLEI GCGC 1 cut(s) 781
AspS9I GGNCC 3 cut(s) 586, 629, 869
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 1 cut(s) 529
AxyI CCTNAGG 1 cut(s) 591
BamHI GGATCC 1 cut(s) 344
BanII GRGCYC 1 cut(s) 747
BauI CACGAG 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 747
BbvI GCAGC 2 cut(s) 157, 820
BccI CCATC 5 cut(s) 149, 577, 801, 890, 941
BciT130I CCWGG 3 cut(s) 91, 198, 699
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 2 cut(s) 8, 397
BfuAI ACCTGC 1 cut(s) 676
BisI GCNGC 2 cut(s) 171, 834
BlpI GCTNAGC 1 cut(s) 837
BlsI GCNGC 2 cut(s) 172, 835
Bme1390I CCNGG 4 cut(s) 91, 198, 207, 699
BmeRI GACNNNNNGTC 2 cut(s) 99, 957
BmgBI CACGTC 1 cut(s) 864
BmgT120I GGNCC 3 cut(s) 586, 629, 869
BmiI GGNNCC 5 cut(s) 346, 588, 650, 690, 708
BmrFI CCNGG 4 cut(s) 91, 198, 207, 699
BmsI GCATC 1 cut(s) 478
BpmI CTGGAG 1 cut(s) 618
Bpu1102I GCTNAGC 1 cut(s) 837
BpuMI CCSGG 1 cut(s) 207
BsaJI CCNNGG 5 cut(s) 174, 206, 619, 652, 697
BsaXI ACNNNNNCTCC 6 cut(s) 202, 230, 232, 260, 640, 670
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 698, 703
Bse21I CCTNAGG 1 cut(s) 591
Bse3DI GCAATG 2 cut(s) 185, 741
BseBI CCWGG 3 cut(s) 91, 198, 699
BseDI CCNNGG 5 cut(s) 174, 206, 619, 652, 697
BseGI GGATG 4 cut(s) 493, 588, 789, 850
BseLI CCNNNNNNNGG 3 cut(s) 448, 698, 703
BseMI GCAATG 2 cut(s) 185, 741
BseMII CTCAG 2 cut(s) 582, 851
BseRI GAGGAG 1 cut(s) 226
BseXI GCAGC 2 cut(s) 157, 820
BseYI CCCAGC 1 cut(s) 34
BsgI GTGCAG 1 cut(s) 87
Bsh1236I CGCG 1 cut(s) 781
BshFI GGCC 3 cut(s) 587, 631, 871
BsiHKAI GWGCWC 1 cut(s) 747
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 2 cut(s) 583, 964
BslI CCNNNNNNNGG 3 cut(s) 448, 698, 703
BsmAI GTCTC 2 cut(s) 8, 397
BsmBI CGTCTC 1 cut(s) 8
BsmFI GGGAC 2 cut(s) 583, 964
BsmI GAATGC 3 cut(s) 29, 31, 575
BsnI GGCC 3 cut(s) 587, 631, 871
Bsp1286I GDGCHC 1 cut(s) 747
Bsp143I GATC 5 cut(s) 143, 193, 344, 368, 497
Bsp1720I GCTNAGC 1 cut(s) 837
BspANI GGCC 3 cut(s) 587, 631, 871
BspCNI CTCAG 2 cut(s) 583, 850
BspFNI CGCG 1 cut(s) 781
BspLI GGNNCC 5 cut(s) 346, 588, 650, 690, 708
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 5 cut(s) 151, 188, 339, 352, 505
BsrDI GCAATG 2 cut(s) 185, 741
BssECI CCNNGG 5 cut(s) 174, 206, 619, 652, 697
BssMI GATC 5 cut(s) 143, 193, 344, 368, 497
BssSI CACGAG 1 cut(s) 387
BssT1I CCWWGG 3 cut(s) 174, 619, 652
Bst2BI CACGAG 1 cut(s) 387
Bst2UI CCWGG 3 cut(s) 91, 198, 699
Bst4CI ACNGT 3 cut(s) 664, 711, 927
BstC8I GCNNGC 2 cut(s) 333, 510
BstDEI CTNAG 3 cut(s) 251, 591, 837
BstF5I GGATG 4 cut(s) 493, 588, 789, 850
BstFNI CGCG 1 cut(s) 781
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 5 cut(s) 146, 196, 347, 371, 500
BstMAI GTCTC 2 cut(s) 8, 397
BstMBI GATC 5 cut(s) 143, 193, 344, 368, 497
BstMWI GCNNNNNNNGC 4 cut(s) 35, 126, 509, 657
BstNI CCWGG 3 cut(s) 91, 198, 699
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 4 cut(s) 89, 196, 205, 697
BstUI CGCG 1 cut(s) 781
BstV1I GCAGC 2 cut(s) 157, 820
BstX2I RGATCY 3 cut(s) 143, 344, 497
BstXI CCANNNNNNTGG 2 cut(s) 90, 626
BstYI RGATCY 3 cut(s) 143, 344, 497
Bsu36I CCTNAGG 1 cut(s) 591
BsuRI GGCC 3 cut(s) 587, 631, 871
BtrI CACGTC 1 cut(s) 864
BtsCI GGATG 4 cut(s) 493, 588, 789, 850
BtsI GCAGTG 2 cut(s) 75, 828
BtsIMutI CAGTG 4 cut(s) 75, 660, 803, 828
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 2 cut(s) 333, 510
CfoI GCGC 1 cut(s) 781
Cfr13I GGNCC 3 cut(s) 586, 629, 869
Csp6I GTAC 2 cut(s) 4, 694
CviAII CATG 3 cut(s) 332, 437, 887
CviQI GTAC 2 cut(s) 4, 694
DdeI CTNAG 3 cut(s) 251, 591, 837
DpnI GATC 5 cut(s) 145, 195, 346, 370, 499
DpnII GATC 5 cut(s) 143, 193, 344, 368, 497
DriI GACNNNNNGTC 2 cut(s) 99, 957
Eam1105I GACNNNNNGTC 2 cut(s) 99, 957
Ecl136II GAGCTC 1 cut(s) 745
Eco130I CCWWGG 3 cut(s) 174, 619, 652
Eco24I GRGCYC 1 cut(s) 747
Eco32I GATATC 1 cut(s) 463
Eco53kI GAGCTC 1 cut(s) 745
Eco57I CTGAAG 1 cut(s) 135
Eco81I CCTNAGG 1 cut(s) 591
EcoICRI GAGCTC 1 cut(s) 745
EcoRII CCWGG 3 cut(s) 89, 196, 697
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 3 cut(s) 174, 619, 652
EcoT22I ATGCAT 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 747
ErhI CCWWGG 3 cut(s) 174, 619, 652
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 3 cut(s) 335, 440, 890
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FaqI GGGAC 2 cut(s) 583, 964
FatI CATG 3 cut(s) 331, 436, 886
FblI GTMKAC 1 cut(s) 802
Fnu4HI GCNGC 2 cut(s) 171, 834
FokI GGATG 4 cut(s) 500, 595, 776, 837
FriOI GRGCYC 1 cut(s) 747
Fsp4HI GCNGC 2 cut(s) 171, 834
GlaI GCGC 1 cut(s) 780
GluI GCNGC 2 cut(s) 171, 834
GsaI CCCAGC 1 cut(s) 38
GsuI CTGGAG 1 cut(s) 618
HaeIII GGCC 3 cut(s) 587, 631, 871
HapII CCGG 1 cut(s) 206
HhaI GCGC 1 cut(s) 781
Hin1II CATG 3 cut(s) 335, 440, 890
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HincII GTYRAC 1 cut(s) 678
HindII GTYRAC 1 cut(s) 678
HindIII AAGCTT 1 cut(s) 506
HinfI GANTC 1 cut(s) 412
HpaI GTTAAC 1 cut(s) 678
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 3 cut(s) 454, 678, 803
Hpy188I TCNGA 2 cut(s) 154, 366
Hpy188III TCNNGA 1 cut(s) 580
Hpy8I GTNNAC 3 cut(s) 454, 678, 803
HpyAV CCTTC 1 cut(s) 907
HpyCH4III ACNGT 3 cut(s) 664, 711, 927
HpyCH4IV ACGT 2 cut(s) 753, 863
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 126, 509, 657
HpyF3I CTNAG 3 cut(s) 251, 591, 837
HpySE526I ACGT 2 cut(s) 753, 863
Hsp92II CATG 3 cut(s) 335, 440, 890
HspAI GCGC 1 cut(s) 779
KspAI GTTAAC 1 cut(s) 678
Kzo9I GATC 5 cut(s) 143, 193, 344, 368, 497
LmnI GCTCC 5 cut(s) 213, 328, 648, 742, 750
Lsp1109I GCAGC 2 cut(s) 157, 820
LweI GCATC 1 cut(s) 478
MaeII ACGT 2 cut(s) 753, 863
MaeIII GTNAC 1 cut(s) 859
MalI GATC 5 cut(s) 145, 195, 346, 370, 499
MboI GATC 5 cut(s) 143, 193, 344, 368, 497
MboII GAAGA 5 cut(s) 60, 138, 141, 407, 625
MflI RGATCY 3 cut(s) 143, 344, 497
MhlI GDGCHC 1 cut(s) 747
MluCI AATT 3 cut(s) 20, 59, 552
MmeI TCCRAC 1 cut(s) 648
MnlI CCTC 7 cut(s) 204, 586, 642, 759, 768, 769, 929
Mph1103I ATGCAT 1 cut(s) 31
MroXI GAANNNNTTC 2 cut(s) 29, 711
MseI TTAA 5 cut(s) 279, 677, 726, 810, 970
MslI CAYNNNNRTG 1 cut(s) 542
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 4 cut(s) 91, 198, 207, 699
Mva1269I GAATGC 3 cut(s) 29, 31, 575
MvaI CCWGG 3 cut(s) 91, 198, 699
MvnI CGCG 1 cut(s) 781
MwoI GCNNNNNNNGC 4 cut(s) 35, 126, 509, 657
NciI CCSGG 1 cut(s) 207
NdeII GATC 5 cut(s) 143, 193, 344, 368, 497
NlaIII CATG 3 cut(s) 335, 440, 890
NlaIV GGNNCC 5 cut(s) 346, 588, 650, 690, 708
NmuCI GTSAC 1 cut(s) 859
NsiI ATGCAT 1 cut(s) 31
NspI RCATGY 1 cut(s) 335
PaeI GCATGC 1 cut(s) 335
PctI GAATGC 3 cut(s) 29, 31, 575
PdmI GAANNNNTTC 2 cut(s) 29, 711
PfeI GAWTC 1 cut(s) 412
PflMI CCANNNNNTGG 1 cut(s) 448
PfoI TCCNGGA 1 cut(s) 196
PkrI GCNGC 2 cut(s) 172, 835
Psp124BI GAGCTC 1 cut(s) 747
Psp6I CCWGG 3 cut(s) 89, 196, 697
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 3 cut(s) 89, 196, 697
PspN4I GGNNCC 5 cut(s) 346, 588, 650, 690, 708
PspPI GGNCC 3 cut(s) 586, 629, 869
PsuI RGATCY 3 cut(s) 143, 344, 497
RsaI GTAC 2 cut(s) 5, 695
RsaNI GTAC 2 cut(s) 4, 694
RseI CAYNNNNRTG 1 cut(s) 542
SacI GAGCTC 1 cut(s) 747
SaqAI TTAA 5 cut(s) 279, 677, 726, 810, 970
SatI GCNGC 2 cut(s) 171, 834
Sau3AI GATC 5 cut(s) 143, 193, 344, 368, 497
Sau96I GGNCC 3 cut(s) 586, 629, 869
ScrFI CCNGG 4 cut(s) 91, 198, 207, 699
SduI GDGCHC 1 cut(s) 747
SfaNI GCATC 1 cut(s) 478
SmiMI CAYNNNNRTG 1 cut(s) 542
SphI GCATGC 1 cut(s) 335
Sse9I AATT 3 cut(s) 20, 59, 552
SspI AATATT 1 cut(s) 478
SstI GAGCTC 1 cut(s) 747
StyD4I CCNGG 4 cut(s) 89, 196, 205, 697
StyI CCWWGG 3 cut(s) 174, 619, 652
TaaI ACNGT 3 cut(s) 664, 711, 927
TaiI ACGT 2 cut(s) 756, 866
TaqI TCGA 3 cut(s) 352, 823, 894
TaqII GACCGA 2 cut(s) 121, 410
TasI AATT 3 cut(s) 20, 59, 552
TfiI GAWTC 1 cut(s) 412
Tru1I TTAA 5 cut(s) 279, 677, 726, 810, 970
Tru9I TTAA 5 cut(s) 279, 677, 726, 810, 970
TscAI CASTG 4 cut(s) 75, 667, 803, 835
TseFI GTSAC 1 cut(s) 859
TseI GCWGC 2 cut(s) 170, 833
Tsp45I GTSAC 1 cut(s) 859
TspDTI ATGAA 8 cut(s) 174, 369, 560, 564, 626, 778, 926, 936
TspGWI ACGGA 1 cut(s) 681
TspRI CASTG 4 cut(s) 75, 667, 803, 835
Van91I CCANNNNNTGG 1 cut(s) 448
XapI RAATTY 1 cut(s) 552
XceI RCATGY 1 cut(s) 335
XcmI CCANNNNNNNNNTGG 2 cut(s) 642, 893
XmiI GTMKAC 1 cut(s) 802
XmnI GAANNNNTTC 2 cut(s) 29, 711
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.