Mitogen-activated protein kinase kinase kinase 1-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 489 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1414 |
| AccI | GTMKAC | 1 cut(s) | 56 |
| AccII | CGCG | 2 cut(s) | 143, 243 |
| AccIII | TCCGGA | 2 cut(s) | 189, 346 |
| AclWI | GGATC | 3 cut(s) | 931, 1084, 1479 |
| AcsI | RAATTY | 2 cut(s) | 263, 1443 |
| AcyI | GRCGYC | 3 cut(s) | 96, 165, 486 |
| AfaI | GTAC | 2 cut(s) | 176, 871 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1414 |
| AgsI | TTSAA | 5 cut(s) | 842, 860, 945, 1006, 1432 |
| AjnI | CCWGG | 4 cut(s) | 14, 48, 783, 1272 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1138, 1170 |
| AluBI | AGCT | 8 cut(s) | 237, 492, 685, 710, 892, 910, 1208, 1411 |
| AluI | AGCT | 8 cut(s) | 237, 492, 685, 710, 892, 910, 1208, 1411 |
| Alw21I | GWGCWC | 1 cut(s) | 1032 |
| Alw26I | GTCTC | 4 cut(s) | 118, 290, 941, 976 |
| AlwI | GGATC | 3 cut(s) | 931, 1084, 1479 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1457 |
| Aor13HI | TCCGGA | 2 cut(s) | 189, 346 |
| AoxI | GGCC | 5 cut(s) | 407, 467, 1161, 1394, 1436 |
| ApeKI | GCWGC | 3 cut(s) | 625, 1205, 1403 |
| ApoI | RAATTY | 2 cut(s) | 263, 1443 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 248, 280 |
| AspA2I | CCTAGG | 1 cut(s) | 353 |
| AspS9I | GGNCC | 2 cut(s) | 408, 1162 |
| AsuHPI | GGTGA | 3 cut(s) | 301, 1262, 1322 |
| AvrII | CCTAGG | 1 cut(s) | 353 |
| BbsI | GAAGAC | 1 cut(s) | 657 |
| Bbv12I | GWGCWC | 1 cut(s) | 1032 |
| BbvI | GCAGC | 3 cut(s) | 637, 1217, 1415 |
| BccI | CCATC | 2 cut(s) | 728, 1153 |
| BceAI | ACGGC | 2 cut(s) | 274, 353 |
| BciT130I | CCWGG | 4 cut(s) | 16, 50, 785, 1274 |
| BclI | TGATCA | 1 cut(s) | 772 |
| BcoDI | GTCTC | 4 cut(s) | 118, 290, 941, 976 |
| BfaI | CTAG | 1 cut(s) | 354 |
| BfmI | CTRYAG | 1 cut(s) | 1398 |
| BisI | GCNGC | 6 cut(s) | 99, 280, 382, 626, 1206, 1404 |
| BlnI | CCTAGG | 1 cut(s) | 353 |
| BlsI | GCNGC | 6 cut(s) | 100, 281, 383, 627, 1207, 1405 |
| BmcAI | AGTACT | 1 cut(s) | 176 |
| Bme1390I | CCNGG | 4 cut(s) | 16, 50, 785, 1274 |
| BmgT120I | GGNCC | 2 cut(s) | 408, 1162 |
| BmiI | GGNNCC | 4 cut(s) | 351, 410, 700, 1164 |
| BmrFI | CCNGG | 4 cut(s) | 16, 50, 785, 1274 |
| BmsI | GCATC | 4 cut(s) | 261, 1057, 1336, 1429 |
| BpiI | GAAGAC | 1 cut(s) | 657 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 949, 981 |
| BpmI | CTGGAG | 1 cut(s) | 1434 |
| BpuEI | CTTGAG | 4 cut(s) | 830, 926, 951, 976 |
| BsaHI | GRCGYC | 3 cut(s) | 96, 165, 486 |
| BsaJI | CCNNGG | 3 cut(s) | 353, 784, 1107 |
| BsaWI | WCCGGW | 2 cut(s) | 189, 346 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 296, 326, 517, 547 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1414 |
| Bse118I | RCCGGY | 2 cut(s) | 415, 1392 |
| Bse1I | ACTGG | 2 cut(s) | 382, 1451 |
| BseAI | TCCGGA | 2 cut(s) | 189, 346 |
| BseBI | CCWGG | 4 cut(s) | 16, 50, 785, 1274 |
| BseDI | CCNNGG | 3 cut(s) | 353, 784, 1107 |
| BseGI | GGATG | 5 cut(s) | 886, 1072, 1164, 1232, 1420 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1414 |
| BseNI | ACTGG | 2 cut(s) | 382, 1451 |
| BseRI | GAGGAG | 3 cut(s) | 204, 289, 402 |
| BseXI | GCAGC | 3 cut(s) | 637, 1217, 1415 |
| BseYI | CCCAGC | 1 cut(s) | 1407 |
| BsgI | GTGCAG | 1 cut(s) | 644 |
| Bsh1236I | CGCG | 2 cut(s) | 143, 243 |
| Bsh1285I | CGRYCG | 2 cut(s) | 133, 366 |
| BshFI | GGCC | 5 cut(s) | 409, 469, 1163, 1396, 1438 |
| BsiEI | CGRYCG | 2 cut(s) | 133, 366 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1032 |
| BsiSI | CCGG | 5 cut(s) | 190, 347, 406, 416, 1393 |
| BslFI | GGGAC | 1 cut(s) | 1439 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1414 |
| BsmAI | GTCTC | 4 cut(s) | 118, 290, 941, 976 |
| BsmFI | GGGAC | 1 cut(s) | 1439 |
| BsnI | GGCC | 5 cut(s) | 409, 469, 1163, 1396, 1438 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1032 |
| Bsp13I | TCCGGA | 2 cut(s) | 189, 346 |
| Bsp143I | GATC | 9 cut(s) | 130, 144, 169, 772, 923, 1018, 1076, 1299, 1484 |
| BspANI | GGCC | 5 cut(s) | 409, 469, 1163, 1396, 1438 |
| BspEI | TCCGGA | 2 cut(s) | 189, 346 |
| BspFNI | CGCG | 2 cut(s) | 143, 243 |
| BspHI | TCATGA | 1 cut(s) | 1015 |
| BspLI | GGNNCC | 4 cut(s) | 351, 410, 700, 1164 |
| BspPI | GGATC | 3 cut(s) | 931, 1084, 1479 |
| BspQI | GCTCTTC | 1 cut(s) | 499 |
| BsrFI | RCCGGY | 2 cut(s) | 415, 1392 |
| BsrI | ACTGG | 2 cut(s) | 382, 1451 |
| BssAI | RCCGGY | 2 cut(s) | 415, 1392 |
| BssECI | CCNNGG | 3 cut(s) | 353, 784, 1107 |
| BssMI | GATC | 9 cut(s) | 130, 144, 169, 772, 923, 1018, 1076, 1299, 1484 |
| BssNI | GRCGYC | 3 cut(s) | 96, 165, 486 |
| BssT1I | CCWWGG | 2 cut(s) | 353, 1107 |
| Bst2UI | CCWGG | 4 cut(s) | 16, 50, 785, 1274 |
| Bst4CI | ACNGT | 1 cut(s) | 1478 |
| Bst6I | CTCTTC | 2 cut(s) | 498, 499 |
| BstACI | GRCGYC | 3 cut(s) | 96, 165, 486 |
| BstC8I | GCNNGC | 3 cut(s) | 1203, 1283, 1287 |
| BstF5I | GGATG | 5 cut(s) | 886, 1072, 1164, 1232, 1420 |
| BstFNI | CGCG | 2 cut(s) | 143, 243 |
| BstKTI | GATC | 9 cut(s) | 133, 147, 172, 775, 926, 1021, 1079, 1302, 1487 |
| BstMAI | GTCTC | 4 cut(s) | 118, 290, 941, 976 |
| BstMBI | GATC | 9 cut(s) | 130, 144, 169, 772, 923, 1018, 1076, 1299, 1484 |
| BstMCI | CGRYCG | 2 cut(s) | 133, 366 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 118, 249, 258, 288, 1417 |
| BstNI | CCWGG | 4 cut(s) | 16, 50, 785, 1274 |
| BstNSI | RCATGY | 1 cut(s) | 1285 |
| BstSCI | CCNGG | 4 cut(s) | 14, 48, 783, 1272 |
| BstSFI | CTRYAG | 1 cut(s) | 1398 |
| BstUI | CGCG | 2 cut(s) | 143, 243 |
| BstV1I | GCAGC | 3 cut(s) | 637, 1217, 1415 |
| BstV2I | GAAGAC | 1 cut(s) | 657 |
| BstX2I | RGATCY | 1 cut(s) | 1076 |
| BstYI | RGATCY | 1 cut(s) | 1076 |
| BsuRI | GGCC | 5 cut(s) | 409, 469, 1163, 1396, 1438 |
| BtsCI | GGATG | 5 cut(s) | 886, 1072, 1164, 1232, 1420 |
| BtsI | GCAGTG | 1 cut(s) | 661 |
| BtsIMutI | CAGTG | 1 cut(s) | 661 |
| Cac8I | GCNNGC | 3 cut(s) | 1203, 1283, 1287 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1457 |
| CciI | TCATGA | 1 cut(s) | 1015 |
| Cfr10I | RCCGGY | 2 cut(s) | 415, 1392 |
| Cfr13I | GGNCC | 2 cut(s) | 408, 1162 |
| CseI | GACGC | 4 cut(s) | 104, 173, 232, 660 |
| CsiI | ACCWGGT | 1 cut(s) | 14 |
| Csp6I | GTAC | 2 cut(s) | 175, 870 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 446, 481 |
| CviAII | CATG | 5 cut(s) | 91, 548, 845, 1016, 1282 |
| CviQI | GTAC | 2 cut(s) | 175, 870 |
| DpnI | GATC | 9 cut(s) | 132, 146, 171, 774, 925, 1020, 1078, 1301, 1486 |
| DpnII | GATC | 9 cut(s) | 130, 144, 169, 772, 923, 1018, 1076, 1299, 1484 |
| Eam1104I | CTCTTC | 2 cut(s) | 498, 499 |
| EarI | CTCTTC | 2 cut(s) | 498, 499 |
| EciI | GGCGGA | 2 cut(s) | 317, 399 |
| Eco130I | CCWWGG | 2 cut(s) | 353, 1107 |
| Eco147I | AGGCCT | 1 cut(s) | 1438 |
| Eco32I | GATATC | 1 cut(s) | 1042 |
| EcoRI | GAATTC | 1 cut(s) | 263 |
| EcoRII | CCWGG | 4 cut(s) | 14, 48, 783, 1272 |
| EcoRV | GATATC | 1 cut(s) | 1042 |
| EcoT14I | CCWWGG | 2 cut(s) | 353, 1107 |
| ErhI | CCWWGG | 2 cut(s) | 353, 1107 |
| FaeI | CATG | 5 cut(s) | 94, 551, 848, 1019, 1285 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 912, 944, 938, 970 |
| FaqI | GGGAC | 1 cut(s) | 1439 |
| FatI | CATG | 5 cut(s) | 90, 547, 844, 1015, 1281 |
| FauI | CCCGC | 2 cut(s) | 323, 420 |
| FauNDI | CATATG | 1 cut(s) | 1214 |
| FbaI | TGATCA | 1 cut(s) | 772 |
| FblI | GTMKAC | 1 cut(s) | 56 |
| Fnu4HI | GCNGC | 6 cut(s) | 99, 280, 382, 626, 1206, 1404 |
| FokI | GGATG | 5 cut(s) | 893, 1079, 1171, 1239, 1407 |
| Fsp4HI | GCNGC | 6 cut(s) | 99, 280, 382, 626, 1206, 1404 |
| FspBI | CTAG | 1 cut(s) | 354 |
| GluI | GCNGC | 6 cut(s) | 99, 280, 382, 626, 1206, 1404 |
| GsaI | CCCAGC | 1 cut(s) | 1411 |
| GsuI | CTGGAG | 1 cut(s) | 1434 |
| HaeIII | GGCC | 5 cut(s) | 409, 469, 1163, 1396, 1438 |
| HapII | CCGG | 5 cut(s) | 190, 347, 406, 416, 1393 |
| HgaI | GACGC | 4 cut(s) | 104, 173, 232, 660 |
| Hin1I | GRCGYC | 3 cut(s) | 96, 165, 486 |
| Hin1II | CATG | 5 cut(s) | 94, 551, 848, 1019, 1285 |
| HincII | GTYRAC | 3 cut(s) | 57, 1248, 1381 |
| HindII | GTYRAC | 3 cut(s) | 57, 1248, 1381 |
| HpaI | GTTAAC | 2 cut(s) | 1248, 1381 |
| HpaII | CCGG | 5 cut(s) | 190, 347, 406, 416, 1393 |
| HphI | GGTGA | 3 cut(s) | 301, 1262, 1322 |
| Hpy166II | GTNNAC | 6 cut(s) | 57, 981, 1248, 1373, 1381, 1481 |
| Hpy188I | TCNGA | 8 cut(s) | 117, 248, 304, 499, 565, 650, 1299, 1304 |
| Hpy8I | GTNNAC | 6 cut(s) | 57, 981, 1248, 1373, 1381, 1481 |
| Hpy99I | CGWCG | 4 cut(s) | 167, 260, 341, 344 |
| HpyAV | CCTTC | 6 cut(s) | 209, 524, 713, 1176, 1271, 1299 |
| HpyCH4III | ACNGT | 1 cut(s) | 1478 |
| HpyCH4IV | ACGT | 3 cut(s) | 342, 486, 634 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 118, 249, 258, 288, 1417 |
| HpySE526I | ACGT | 3 cut(s) | 342, 486, 634 |
| Hsp92I | GRCGYC | 3 cut(s) | 96, 165, 486 |
| Hsp92II | CATG | 5 cut(s) | 94, 551, 848, 1019, 1285 |
| Kpn2I | TCCGGA | 2 cut(s) | 189, 346 |
| Ksp22I | TGATCA | 1 cut(s) | 772 |
| KspAI | GTTAAC | 2 cut(s) | 1248, 1381 |
| Kzo9I | GATC | 9 cut(s) | 130, 144, 169, 772, 923, 1018, 1076, 1299, 1484 |
| LguI | GCTCTTC | 1 cut(s) | 499 |
| LmnI | GCTCC | 3 cut(s) | 707, 1035, 1417 |
| Lsp1109I | GCAGC | 3 cut(s) | 637, 1217, 1415 |
| LweI | GCATC | 4 cut(s) | 261, 1057, 1336, 1429 |
| MabI | ACCWGGT | 1 cut(s) | 14 |
| MaeI | CTAG | 1 cut(s) | 354 |
| MaeII | ACGT | 3 cut(s) | 342, 486, 634 |
| MaeIII | GTNAC | 2 cut(s) | 358, 913 |
| MalI | GATC | 9 cut(s) | 132, 146, 171, 774, 925, 1020, 1078, 1301, 1486 |
| MboI | GATC | 9 cut(s) | 130, 144, 169, 772, 923, 1018, 1076, 1299, 1484 |
| MboII | GAAGA | 7 cut(s) | 97, 286, 423, 486, 515, 657, 1004 |
| MflI | RGATCY | 1 cut(s) | 1076 |
| MhlI | GDGCHC | 1 cut(s) | 1032 |
| MluCI | AATT | 5 cut(s) | 263, 463, 589, 1323, 1443 |
| MlyI | GAGTC | 6 cut(s) | 52, 62, 120, 566, 805, 1228 |
| MmeI | TCCRAC | 2 cut(s) | 327, 1218 |
| MroI | TCCGGA | 2 cut(s) | 189, 346 |
| MseI | TTAA | 5 cut(s) | 425, 830, 1176, 1247, 1380 |
| MslI | CAYNNNNRTG | 1 cut(s) | 591 |
| MspA1I | CMGCKG | 1 cut(s) | 1208 |
| MspI | CCGG | 5 cut(s) | 190, 347, 406, 416, 1393 |
| MspR9I | CCNGG | 4 cut(s) | 16, 50, 785, 1274 |
| MvaI | CCWGG | 4 cut(s) | 16, 50, 785, 1274 |
| MvnI | CGCG | 2 cut(s) | 143, 243 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 118, 249, 258, 288, 1417 |
| NdeI | CATATG | 1 cut(s) | 1214 |
| NdeII | GATC | 9 cut(s) | 130, 144, 169, 772, 923, 1018, 1076, 1299, 1484 |
| NlaIII | CATG | 5 cut(s) | 94, 551, 848, 1019, 1285 |
| NlaIV | GGNNCC | 4 cut(s) | 351, 410, 700, 1164 |
| NmeAIII | GCCGAG | 2 cut(s) | 147, 267 |
| NspI | RCATGY | 1 cut(s) | 1285 |
| PaeI | GCATGC | 1 cut(s) | 1285 |
| PagI | TCATGA | 1 cut(s) | 1015 |
| PceI | AGGCCT | 1 cut(s) | 1438 |
| PciSI | GCTCTTC | 1 cut(s) | 499 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 264, 1186 |
| PfeI | GAWTC | 6 cut(s) | 560, 738, 820, 962, 1186, 1331 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1414 |
| PkrI | GCNGC | 6 cut(s) | 100, 281, 383, 627, 1207, 1405 |
| Ple19I | CGATCG | 1 cut(s) | 133 |
| PleI | GAGTC | 6 cut(s) | 52, 61, 120, 566, 804, 1227 |
| PpsI | GAGTC | 6 cut(s) | 52, 61, 120, 566, 804, 1227 |
| Psp6I | CCWGG | 4 cut(s) | 14, 48, 783, 1272 |
| PspFI | CCCAGC | 1 cut(s) | 1407 |
| PspGI | CCWGG | 4 cut(s) | 14, 48, 783, 1272 |
| PspN4I | GGNNCC | 4 cut(s) | 351, 410, 700, 1164 |
| PspPI | GGNCC | 2 cut(s) | 408, 1162 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 143, 175 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1457 |
| PsuI | RGATCY | 1 cut(s) | 1076 |
| PvuI | CGATCG | 1 cut(s) | 133 |
| PvuII | CAGCTG | 1 cut(s) | 1208 |
| RsaI | GTAC | 2 cut(s) | 176, 871 |
| RsaNI | GTAC | 2 cut(s) | 175, 870 |
| RseI | CAYNNNNRTG | 1 cut(s) | 591 |
| SalI | GTCGAC | 1 cut(s) | 55 |
| SapI | GCTCTTC | 1 cut(s) | 499 |
| SaqAI | TTAA | 5 cut(s) | 425, 830, 1176, 1247, 1380 |
| SatI | GCNGC | 6 cut(s) | 99, 280, 382, 626, 1206, 1404 |
| Sau3AI | GATC | 9 cut(s) | 130, 144, 169, 772, 923, 1018, 1076, 1299, 1484 |
| Sau96I | GGNCC | 2 cut(s) | 408, 1162 |
| ScaI | AGTACT | 1 cut(s) | 176 |
| SchI | GAGTC | 6 cut(s) | 52, 62, 120, 566, 805, 1228 |
| ScrFI | CCNGG | 4 cut(s) | 16, 50, 785, 1274 |
| SduI | GDGCHC | 1 cut(s) | 1032 |
| SexAI | ACCWGGT | 1 cut(s) | 14 |
| SfaNI | GCATC | 4 cut(s) | 261, 1057, 1336, 1429 |
| SfcI | CTRYAG | 1 cut(s) | 1398 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 591 |
| SmlI | CTYRAG | 4 cut(s) | 809, 905, 955, 966 |
| SmoI | CTYRAG | 4 cut(s) | 809, 905, 955, 966 |
| SphI | GCATGC | 1 cut(s) | 1285 |
| Sse9I | AATT | 5 cut(s) | 263, 463, 589, 1323, 1443 |
| SseBI | AGGCCT | 1 cut(s) | 1438 |
| SspMI | CTAG | 1 cut(s) | 354 |
| StuI | AGGCCT | 1 cut(s) | 1438 |
| StyD4I | CCNGG | 4 cut(s) | 14, 48, 783, 1272 |
| StyI | CCWWGG | 2 cut(s) | 353, 1107 |
| TaaI | ACNGT | 1 cut(s) | 1478 |
| TaiI | ACGT | 3 cut(s) | 345, 489, 637 |
| TaqI | TCGA | 6 cut(s) | 56, 129, 204, 255, 267, 1180 |
| TasI | AATT | 5 cut(s) | 263, 463, 589, 1323, 1443 |
| TatI | WGTACW | 1 cut(s) | 174 |
| TauI | GCSGC | 3 cut(s) | 101, 282, 384 |
| TfiI | GAWTC | 6 cut(s) | 560, 738, 820, 962, 1186, 1331 |
| Tru1I | TTAA | 5 cut(s) | 425, 830, 1176, 1247, 1380 |
| Tru9I | TTAA | 5 cut(s) | 425, 830, 1176, 1247, 1380 |
| TscAI | CASTG | 1 cut(s) | 661 |
| TseI | GCWGC | 3 cut(s) | 625, 1205, 1403 |
| TspDTI | ATGAA | 8 cut(s) | 32, 98, 555, 732, 812, 1004, 1136, 1348 |
| TspGWI | ACGGA | 1 cut(s) | 570 |
| TspRI | CASTG | 1 cut(s) | 661 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1414 |
| XapI | RAATTY | 2 cut(s) | 263, 1443 |
| XceI | RCATGY | 1 cut(s) | 1285 |
| XmaJI | CCTAGG | 1 cut(s) | 353 |
| XmiI | GTMKAC | 1 cut(s) | 56 |
| XspI | CTAG | 1 cut(s) | 354 |
| ZraI | GACGTC | 1 cut(s) | 487 |
| ZrmI | AGTACT | 1 cut(s) | 176 |