Rh5BG540800

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000243
Physical Location & Seq
Reverse (-)
85565616 .. 85690834
125219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG540800.1

Sequence Viewer

Length: 1764 bp
ATGGTGAGGGTTCCGGTGATTGACAATCGATGCTCGACTTGGGACATTTTGAGGGACTTTGCGCCGGAAGAAGAGAGGGAATCGTCGGAAATGGTGCAGAGAAGGTTTGGTTCCTTTTCCTCGTCTTCTTCTGATGATGAAGAAGAAGTTGAAGAAGGAGAGGATGTTGACTTTTCCAGCACTACCGCAGAGATTTCCAGTATTTCTCCAAATGGAAGGTTTTCGCCGAATGGGAAGCCTAAGCTTACTGTCACTAGTTGGGAAAAGGGTGATTTGCTCGGCAGCGGCTCTTTTGGGTCTGTGTATGAAGGAATTTCTGATGGCGGATGCTTCATTGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGGAGGCAAAGAGTTTCTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGGATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAAACGTACACTCTTACTGATTCAATTGTTTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCGAAAAGTGATTCACAGGGACATTAAATGTGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCTGACTTTGGATTGGCAAAGATTATCAAAATGAACGATATAAAATCTTGCCAAGGAACTGCATACTGGATGGCCCCTGAGGTTGTTAATCGTAAGTGTCAAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACGATAATCTCGAATGGGTTGGTGATCTAGCAGTTGAGGAGGCTGCAGAGTTCATGATACAACTGCGCAAAGAGATTCCAGCAGCCATATCTCATACCTTGTTAAAAACAAGGGCTAAATACTGCGGCTATTTCGGGTCTGTGTATGAAGTATTTGCTGATTGCGGAAGCTTCTTTGCTGTCAAGGAAGTTTCCTTGCATGATCAAGGAAGAGTTTCTCGACTTGAACAGGAGATTGCTTTTCTGAGTCAGTTTCAACATCAGAACATAGTTCAGTATTATGGCACACAAAAGGATGAATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAAACGCACCCTCTTACAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCAAAATGTGATTCACAGGGACATTAAATGCGCAAATATTTTGGTGCATGCTAATGGATCTGTCAAGCTTGCTGACTTTGGATTGGCAAAGGCTATCAAAATGAACGAAAAACTATCTTGCCAAGGAACTGCAAACTGGATGGCCCCTGAGGTTGTTAATCATGAAAGTCAAGGTTATGGCCTTCTGGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACTCTGATCTCGAATGGATGGCGGCATTATGGAAAATTGGAAATGGGAAGCTCCCTCTGGTTCCTGATTCTCTTTCAAGAGAGGCACAAGATTTTATCCGTCTATGCTTACAAGTTGAGCCAGATAATCGTCCCACTGCTGCTCAGCTTTTAGAACATCCATTTGTAAATAAGCCCCTTCCTCCCACATCTTCTGGATCAGTATCTCCTTACAATCACCACAGGCTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

587

Amino Acids

65.75

Weight (kDa)

5.53

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 88 - 288 8.7e-58 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 90 - 289 2.5e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 327 - 566 2.8e-65 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 327 - 562 1.6e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 920, 1318
AccI GTMKAC 2 cut(s) 567, 1245
AciI CCGC 6 cut(s) 186, 285, 324, 978, 1017, 1553
AclWI GGATC 4 cut(s) 481, 673, 1351, 1735
AcsI RAATTY 1 cut(s) 312
AcuI CTGAAG 2 cut(s) 614, 1292
AfaI GTAC 3 cut(s) 539, 820, 1498
AgsI TTSAA 9 cut(s) 152, 401, 431, 521, 555, 1079, 1109, 1199, 1608
AhlI ACTAGT 1 cut(s) 254
AjnI CCWGG 1 cut(s) 372
AjuI GAANNNNNNNTTGG 2 cut(s) 1281, 1313
Alw26I GTCTC 1 cut(s) 1243
AlwI GGATC 4 cut(s) 481, 673, 1351, 1735
AoxI GGCC 4 cut(s) 750, 787, 1428, 1465
ApeKI GCWGC 4 cut(s) 282, 896, 935, 1670
ApoI RAATTY 1 cut(s) 312
Asp700I GAANNNNTTC 2 cut(s) 220, 1066
AspLEI GCGC 3 cut(s) 64, 921, 1319
AspS9I GGNCC 2 cut(s) 751, 1429
AsuHPI GGTGA 5 cut(s) 16, 28, 281, 887, 1739
AxyI CCTNAGG 2 cut(s) 756, 1434
BbsI GAAGAC 1 cut(s) 117
BbvI GCAGC 4 cut(s) 294, 883, 947, 1657
BccI CCATC 7 cut(s) 314, 464, 742, 838, 1420, 1516, 1543
BciT130I CCWGG 1 cut(s) 374
BclI TGATCA 2 cut(s) 361, 1054
BcoDI GTCTC 1 cut(s) 1243
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 4 cut(s) 255, 573, 881, 1251
BfmI CTRYAG 1 cut(s) 897
BglII AGATCT 2 cut(s) 579, 1257
BisI GCNGC 7 cut(s) 283, 286, 897, 936, 979, 1554, 1671
BlpI GCTNAGC 1 cut(s) 1674
BlsI GCNGC 7 cut(s) 284, 287, 898, 937, 980, 1555, 1672
Bme1390I CCNGG 1 cut(s) 374
BmgT120I GGNCC 2 cut(s) 751, 1429
BmiI GGNNCC 9 cut(s) 12, 112, 514, 753, 849, 1192, 1431, 1527, 1593
BmrFI CCNGG 1 cut(s) 374
BmrI ACTGGG 2 cut(s) 849, 1527
BmsI GCATC 2 cut(s) 20, 317
BmuI ACTGGG 2 cut(s) 849, 1527
BpiI GAAGAC 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 1674
Bsa29I ATCGAT 1 cut(s) 28
BsaBI GATNNNNATC 1 cut(s) 1732
BsaJI CCNNGG 3 cut(s) 373, 730, 1408
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bse1I ACTGG 5 cut(s) 198, 749, 844, 1427, 1522
Bse21I CCTNAGG 2 cut(s) 756, 1434
Bse3DI GCAATG 1 cut(s) 333
Bse8I GATNNNNATC 1 cut(s) 1732
BseBI CCWGG 1 cut(s) 374
BseCI ATCGAT 1 cut(s) 28
BseDI CCNNGG 3 cut(s) 373, 730, 1408
BseJI GATNNNNATC 1 cut(s) 1732
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 6 cut(s) 410, 747, 1088, 1425, 1688, 1748
BseNI ACTGG 5 cut(s) 198, 749, 844, 1427, 1522
BseRI GAGGAG 1 cut(s) 905
BseXI GCAGC 4 cut(s) 294, 883, 947, 1657
BsgI GTGCAG 1 cut(s) 116
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 4 cut(s) 752, 789, 1430, 1467
BshVI ATCGAT 1 cut(s) 28
BsiEI CGRYCG 1 cut(s) 610
BsiSI CCGG 2 cut(s) 14, 65
BslFI GGGAC 5 cut(s) 56, 68, 641, 1319, 1647
BsmAI GTCTC 1 cut(s) 1243
BsmFI GGGAC 5 cut(s) 56, 68, 641, 1319, 1647
BsnI GGCC 4 cut(s) 752, 789, 1430, 1467
Bsp1720I GCTNAGC 1 cut(s) 1674
BspACI CCGC 6 cut(s) 186, 285, 324, 978, 1017, 1553
BspANI GGCC 4 cut(s) 752, 789, 1430, 1467
BspCNI CTCAG 6 cut(s) 411, 748, 1089, 1426, 1687, 1749
BspDI ATCGAT 1 cut(s) 28
BspHI TCATGA 2 cut(s) 906, 1447
BspLI GGNNCC 9 cut(s) 12, 112, 514, 753, 849, 1192, 1431, 1527, 1593
BspMAI CTGCAG 1 cut(s) 901
BspPI GGATC 4 cut(s) 481, 673, 1351, 1735
BspQI GCTCTTC 1 cut(s) 420
BsrDI GCAATG 1 cut(s) 333
BsrI ACTGG 5 cut(s) 198, 749, 844, 1427, 1522
BssECI CCNNGG 3 cut(s) 373, 730, 1408
BssNAI GTATAC 2 cut(s) 568, 1246
BssT1I CCWWGG 2 cut(s) 730, 1408
Bst1107I GTATAC 2 cut(s) 568, 1246
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 3 cut(s) 250, 823, 1501
Bst6I CTCTTC 3 cut(s) 66, 420, 1057
BstC8I GCNNGC 4 cut(s) 657, 678, 1335, 1356
BstDEI CTNAG 7 cut(s) 240, 419, 756, 1097, 1434, 1674, 1757
BstHHI GCGC 3 cut(s) 64, 921, 1319
BstMAI GTCTC 1 cut(s) 1243
BstMCI CGRYCG 1 cut(s) 610
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 3 cut(s) 659, 1238, 1337
BstSCI CCNGG 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 897
BstV1I GCAGC 4 cut(s) 294, 883, 947, 1657
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 4 cut(s) 579, 665, 1257, 1343
BstYI RGATCY 4 cut(s) 579, 665, 1257, 1343
BstZ17I GTATAC 2 cut(s) 568, 1246
Bsu15I ATCGAT 1 cut(s) 28
Bsu36I CCTNAGG 2 cut(s) 756, 1434
BsuRI GGCC 4 cut(s) 752, 789, 1430, 1467
BsuTUI ATCGAT 1 cut(s) 28
BtsI GCAGTG 1 cut(s) 1665
BtsIMutI CAGTG 1 cut(s) 1665
Cac8I GCNNGC 4 cut(s) 657, 678, 1335, 1356
CciI TCATGA 2 cut(s) 906, 1447
CfoI GCGC 3 cut(s) 64, 921, 1319
Cfr13I GGNCC 2 cut(s) 751, 1429
ClaI ATCGAT 1 cut(s) 28
Csp6I GTAC 3 cut(s) 538, 819, 1497
CviAII CATG 7 cut(s) 434, 656, 907, 1052, 1235, 1334, 1448
CviQI GTAC 3 cut(s) 538, 819, 1497
DdeI CTNAG 7 cut(s) 240, 419, 756, 1097, 1434, 1674, 1757
Eam1104I CTCTTC 3 cut(s) 66, 420, 1057
EarI CTCTTC 3 cut(s) 66, 420, 1057
EciI GGCGGA 1 cut(s) 339
Eco130I CCWWGG 2 cut(s) 730, 1408
Eco57I CTGAAG 2 cut(s) 614, 1292
Eco81I CCTNAGG 2 cut(s) 756, 1434
EcoRII CCWGG 1 cut(s) 372
EcoT14I CCWWGG 2 cut(s) 730, 1408
ErhI CCWWGG 2 cut(s) 730, 1408
FaeI CATG 7 cut(s) 437, 659, 910, 1055, 1238, 1337, 1451
FalI AAGNNNNNCTT 4 cut(s) 501, 533, 1179, 1211
FaqI GGGAC 5 cut(s) 56, 68, 641, 1319, 1647
FatI CATG 7 cut(s) 433, 655, 906, 1051, 1234, 1333, 1447
FbaI TGATCA 2 cut(s) 361, 1054
FblI GTMKAC 2 cut(s) 567, 1245
Fnu4HI GCNGC 7 cut(s) 283, 286, 897, 936, 979, 1554, 1671
Fsp4HI GCNGC 7 cut(s) 283, 286, 897, 936, 979, 1554, 1671
FspBI CTAG 4 cut(s) 255, 573, 881, 1251
FspI TGCGCA 2 cut(s) 920, 1318
GlaI GCGC 3 cut(s) 63, 920, 1318
GluI GCNGC 7 cut(s) 283, 286, 897, 936, 979, 1554, 1671
HaeIII GGCC 4 cut(s) 752, 789, 1430, 1467
HapII CCGG 2 cut(s) 14, 65
HhaI GCGC 3 cut(s) 64, 921, 1319
Hin1II CATG 7 cut(s) 437, 659, 910, 1055, 1238, 1337, 1451
Hin6I GCGC 3 cut(s) 62, 919, 1317
HinP1I GCGC 3 cut(s) 62, 919, 1317
HincII GTYRAC 3 cut(s) 169, 837, 1515
HindII GTYRAC 3 cut(s) 169, 837, 1515
HindIII AAGCTT 4 cut(s) 242, 674, 1021, 1352
HpaI GTTAAC 2 cut(s) 837, 1515
HpaII CCGG 2 cut(s) 14, 65
HphI GGTGA 5 cut(s) 16, 28, 281, 887, 1739
Hpy166II GTNNAC 6 cut(s) 169, 540, 568, 837, 1246, 1515
Hpy188I TCNGA 9 cut(s) 88, 133, 319, 420, 594, 1098, 1116, 1272, 1537
Hpy8I GTNNAC 6 cut(s) 169, 540, 568, 837, 1246, 1515
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 9 cut(s) 96, 149, 210, 302, 527, 800, 1205, 1478, 1718
HpyCH4III ACNGT 3 cut(s) 250, 823, 1501
HpyCH4IV ACGT 1 cut(s) 536
HpyF3I CTNAG 7 cut(s) 240, 419, 756, 1097, 1434, 1674, 1757
HpySE526I ACGT 1 cut(s) 536
Hsp92II CATG 7 cut(s) 437, 659, 910, 1055, 1238, 1337, 1451
HspAI GCGC 3 cut(s) 62, 919, 1317
Ksp22I TGATCA 2 cut(s) 361, 1054
KspAI GTTAAC 2 cut(s) 837, 1515
LguI GCTCTTC 1 cut(s) 420
LmnI GCTCC 3 cut(s) 518, 1196, 1587
Lsp1109I GCAGC 4 cut(s) 294, 883, 947, 1657
LweI GCATC 2 cut(s) 20, 317
MaeI CTAG 4 cut(s) 255, 573, 881, 1251
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 3 cut(s) 250, 502, 1180
MfeI CAATTG 1 cut(s) 555
MflI RGATCY 4 cut(s) 579, 665, 1257, 1343
MluCI AATT 3 cut(s) 312, 555, 1566
MlyI GAGTC 4 cut(s) 430, 817, 1108, 1495
MmeI TCCRAC 3 cut(s) 66, 807, 1485
MroXI GAANNNNTTC 2 cut(s) 220, 1066
MseI TTAA 7 cut(s) 633, 765, 836, 956, 1311, 1443, 1514
MslI CAYNNNNRTG 2 cut(s) 660, 1338
MspA1I CMGCKG 2 cut(s) 285, 797
MspI CCGG 2 cut(s) 14, 65
MspR9I CCNGG 1 cut(s) 374
MunI CAATTG 1 cut(s) 555
MvaI CCWGG 1 cut(s) 374
NlaIII CATG 7 cut(s) 437, 659, 910, 1055, 1238, 1337, 1451
NlaIV GGNNCC 9 cut(s) 12, 112, 514, 753, 849, 1192, 1431, 1527, 1593
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 250
NsbI TGCGCA 2 cut(s) 920, 1318
NspI RCATGY 3 cut(s) 659, 1238, 1337
PaeI GCATGC 2 cut(s) 659, 1337
PagI TCATGA 2 cut(s) 906, 1447
PciSI GCTCTTC 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 861
PdmI GAANNNNTTC 2 cut(s) 220, 1066
PfeI GAWTC 8 cut(s) 80, 551, 619, 928, 1151, 1229, 1297, 1598
PkrI GCNGC 7 cut(s) 284, 287, 898, 937, 980, 1555, 1672
PleI GAGTC 4 cut(s) 429, 816, 1107, 1494
PpsI GAGTC 4 cut(s) 429, 816, 1107, 1494
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 9 cut(s) 12, 112, 514, 753, 849, 1192, 1431, 1527, 1593
PspPI GGNCC 2 cut(s) 751, 1429
PstI CTGCAG 1 cut(s) 901
PsuI RGATCY 4 cut(s) 579, 665, 1257, 1343
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 3 cut(s) 539, 820, 1498
RsaNI GTAC 3 cut(s) 538, 819, 1497
RseI CAYNNNNRTG 2 cut(s) 660, 1338
SapI GCTCTTC 1 cut(s) 420
SaqAI TTAA 7 cut(s) 633, 765, 836, 956, 1311, 1443, 1514
SatI GCNGC 7 cut(s) 283, 286, 897, 936, 979, 1554, 1671
Sau96I GGNCC 2 cut(s) 751, 1429
SchI GAGTC 4 cut(s) 430, 817, 1108, 1495
ScrFI CCNGG 1 cut(s) 374
SfaNI GCATC 2 cut(s) 20, 317
SfcI CTRYAG 1 cut(s) 897
SmiMI CAYNNNNRTG 2 cut(s) 660, 1338
SpeI ACTAGT 1 cut(s) 254
SphI GCATGC 2 cut(s) 659, 1337
Sse9I AATT 3 cut(s) 312, 555, 1566
SsiI CCGC 6 cut(s) 186, 285, 324, 978, 1017, 1553
SspI AATATT 1 cut(s) 1324
SspMI CTAG 4 cut(s) 255, 573, 881, 1251
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 2 cut(s) 730, 1408
TaaI ACNGT 3 cut(s) 250, 823, 1501
TaiI ACGT 1 cut(s) 539
TaqI TCGA 5 cut(s) 28, 35, 864, 1072, 1542
TaqII GACCGA 1 cut(s) 624
TasI AATT 3 cut(s) 312, 555, 1566
TatI WGTACW 2 cut(s) 818, 1496
TauI GCSGC 3 cut(s) 288, 981, 1556
TfiI GAWTC 8 cut(s) 80, 551, 619, 928, 1151, 1229, 1297, 1598
Tru1I TTAA 7 cut(s) 633, 765, 836, 956, 1311, 1443, 1514
Tru9I TTAA 7 cut(s) 633, 765, 836, 956, 1311, 1443, 1514
TscAI CASTG 1 cut(s) 1672
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 4 cut(s) 282, 896, 935, 1670
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 9 cut(s) 153, 321, 322, 725, 895, 1014, 1164, 1403, 1464
TspGWI ACGGA 1 cut(s) 1619
TspRI CASTG 1 cut(s) 1672
XapI RAATTY 1 cut(s) 312
XceI RCATGY 3 cut(s) 659, 1238, 1337
XcmI CCANNNNNNNNNTGG 1 cut(s) 801
XmiI GTMKAC 2 cut(s) 567, 1245
XmnI GAANNNNTTC 2 cut(s) 220, 1066
XspI CTAG 4 cut(s) 255, 573, 881, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.