Rw5G048230

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000243
Physical Location & Seq
Reverse (-)
83750252 .. 83755117
4866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048230.1

Sequence Viewer

Length: 1200 bp
ATGACGAGGTCACTGTGGATTATGACTGTTGTATCCCAGAGCCCTGACTTAACAAAAATTCAAGGCACATTGGCGGGGCCGAGCGGGTGTTGTACTGCGAATAGTAGTTGTAGTGCAAGTGTCGGAATTAAGGGAGTGCGGCCGCCGGGGCTGAAGCCGCCGCCGTCGATGGTGAGGTTTCCGGTGATTGACAATGGATGCTCGACTTGGGACATTTTGAGGGACTTCGCGCCGGAAGAAGAGAGGGAGTCGTTGGAAATGTTGCAGAGAAGGTTTTGTCCTTTTTCCTTGTCTTCTTCTGATGAAGAAGAAGAACCTTTTTTCTCATGTTCTTCTAATGAAGAAGAAGAATTTTCCAATATTAATGGAAGGTTTTCGCCAAATGGGAAGCTTAAGCTTACTTTCACTAATTGGAAAAAAGGTGTTTTTCTTGGCAGCGGCTCTTTTGGGTCTGTGTATGAAGGAATTTCTGATGGCGGATGCTTCTTTGCTGTGAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGGAGACAAAGAGTTTCTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGAATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAGGGCTCCCTTCAAAAGCTATATCAAATGTACACTCTTACAGATTCTATTGTCTCTGTATACACTAGACAGATCCTATTGTCTCTGGACATTAAATGCGCAAATATTTTGGTGAATGCTGATGGATCTGTGAAGCTTGTTGACTTTGGATTGGCAATGACTATCCAAATGAACGACATAAAATCTTGCCAAGGAACTGTACACTGGATGGCCCCTGAGGTTGTAAATCGTAAGAGTCATGGTTATGGCGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACTCTAATCTCGAATGGATGATGGTATTATGGAAAATTGGAAACGGTGAGCCCCCTCTGGTTCCTGATTCTCTTTCAAGCGAGGCACAAGATTTTATCCGTCTATGCTTACAATGTAAGCCATATAATCGTCCCACTGCTGCTCAGCTTTTAAAACATCCATTTGTAAATAAGCCCCTTCCTCCCACGTCTTCTGGATCGGTATCTCCTTACAATCACCACAGGCAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

399

Amino Acids

44.28

Weight (kDa)

5.52

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 140 - 378 3.9e-53 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 144 - 374 3.5e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 760
AccBSI CCGCTC 1 cut(s) 84
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 230
AciI CCGC 8 cut(s) 74, 84, 139, 143, 158, 161, 438, 477
AclWI GGATC 3 cut(s) 727, 793, 1171
AcoI YGGCCR 1 cut(s) 140
AcsI RAATTY 3 cut(s) 57, 350, 465
AcuI CTGAAG 1 cut(s) 173
AfaI GTAC 4 cut(s) 94, 692, 861, 934
AfiI CCNNNNNNNGG 1 cut(s) 1192
AflII CTTAAG 1 cut(s) 392
AgsI TTSAA 5 cut(s) 62, 554, 584, 674, 1044
AjiI CACGTC 1 cut(s) 1155
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 6 cut(s) 391, 397, 634, 679, 796, 1114
AluI AGCT 6 cut(s) 391, 397, 634, 679, 796, 1114
Alw26I GTCTC 3 cut(s) 526, 718, 747
AlwI GGATC 3 cut(s) 727, 793, 1171
AoxI GGCC 3 cut(s) 77, 140, 870
ApeKI GCWGC 2 cut(s) 435, 1106
ApoI RAATTY 3 cut(s) 57, 350, 465
AseI ATTAAT 1 cut(s) 363
Asp700I GAANNNNTTC 1 cut(s) 373
AspLEI GCGC 3 cut(s) 232, 761, 911
AspS9I GGNCC 2 cut(s) 77, 871
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 5 cut(s) 184, 196, 784, 1025, 1175
AxyI CCTNAGG 1 cut(s) 876
BaeI ACNNNNGTAYC 2 cut(s) 15, 48
BanII GRGCYC 3 cut(s) 44, 668, 1020
BbsI GAAGAC 2 cut(s) 285, 1149
BbvI GCAGC 2 cut(s) 447, 1093
BccI CCATC 6 cut(s) 163, 467, 776, 862, 952, 982
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 1 cut(s) 527
BciVI GTATCC 1 cut(s) 43
BclI TGATCA 1 cut(s) 514
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 3 cut(s) 526, 718, 747
BfaI CTAG 1 cut(s) 726
BfoI RGCGCY 1 cut(s) 912
BfrI CTTAAG 1 cut(s) 392
BfuI GTATCC 1 cut(s) 43
BglI GCCNNNNNGGC 1 cut(s) 148
BisI GCNGC 7 cut(s) 140, 143, 158, 161, 436, 439, 1107
BlpI GCTNAGC 1 cut(s) 1110
BlsI GCNGC 7 cut(s) 141, 144, 159, 162, 437, 440, 1108
Bme1390I CCNGG 2 cut(s) 147, 527
BmgBI CACGTC 1 cut(s) 1155
BmgT120I GGNCC 2 cut(s) 77, 871
BmiI GGNNCC 5 cut(s) 78, 667, 873, 963, 1029
BmrFI CCNGG 2 cut(s) 147, 527
BmrI ACTGGG 1 cut(s) 963
BmsI GCATC 2 cut(s) 188, 470
BmuI ACTGGG 1 cut(s) 963
BpiI GAAGAC 2 cut(s) 285, 1149
Bpu1102I GCTNAGC 1 cut(s) 1110
BpuMI CCSGG 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 1168
BsaJI CCNNGG 4 cut(s) 146, 526, 660, 850
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 1 cut(s) 1192
Bse1I ACTGG 2 cut(s) 869, 958
Bse21I CCTNAGG 1 cut(s) 876
Bse3DI GCAATG 1 cut(s) 822
Bse8I GATNNNNATC 1 cut(s) 1168
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 4 cut(s) 146, 526, 660, 850
BseGI GGATG 8 cut(s) 203, 485, 628, 873, 935, 963, 990, 1123
BseJI GATNNNNATC 1 cut(s) 1168
BseLI CCNNNNNNNGG 1 cut(s) 1192
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 3 cut(s) 563, 867, 1124
BseNI ACTGG 2 cut(s) 869, 958
BseX3I CGGCCG 1 cut(s) 140
BseXI GCAGC 2 cut(s) 447, 1093
Bsh1236I CGCG 1 cut(s) 230
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 3 cut(s) 79, 142, 872
BsiEI CGRYCG 1 cut(s) 143
BsiSI CCGG 3 cut(s) 146, 182, 233
BslFI GGGAC 3 cut(s) 224, 236, 1083
BslI CCNNNNNNNGG 1 cut(s) 1192
BsmAI GTCTC 3 cut(s) 526, 718, 747
BsmFI GGGAC 3 cut(s) 224, 236, 1083
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 3 cut(s) 79, 142, 872
Bsp1286I GDGCHC 3 cut(s) 44, 668, 1020
Bsp1407I TGTACA 2 cut(s) 690, 859
Bsp143I GATC 4 cut(s) 514, 732, 785, 1163
Bsp1720I GCTNAGC 1 cut(s) 1110
BspACI CCGC 8 cut(s) 74, 84, 139, 143, 158, 161, 438, 477
BspANI GGCC 3 cut(s) 79, 142, 872
BspCNI CTCAG 3 cut(s) 564, 868, 1123
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 5 cut(s) 78, 667, 873, 963, 1029
BspPI GGATC 3 cut(s) 727, 793, 1171
BspQI GCTCTTC 1 cut(s) 573
BspTI CTTAAG 1 cut(s) 392
BsrBI CCGCTC 1 cut(s) 84
BsrDI GCAATG 1 cut(s) 822
BsrGI TGTACA 2 cut(s) 690, 859
BsrI ACTGG 2 cut(s) 869, 958
BssECI CCNNGG 4 cut(s) 146, 526, 660, 850
BssMI GATC 4 cut(s) 514, 732, 785, 1163
BssNAI GTATAC 1 cut(s) 721
BssT1I CCWWGG 2 cut(s) 660, 850
Bst1107I GTATAC 1 cut(s) 721
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 5 cut(s) 15, 28, 859, 937, 1013
Bst6I CTCTTC 2 cut(s) 234, 573
BstAFI CTTAAG 1 cut(s) 392
BstAUI TGTACA 2 cut(s) 690, 859
BstDEI CTNAG 3 cut(s) 572, 876, 1110
BstF5I GGATG 8 cut(s) 203, 485, 628, 873, 935, 963, 990, 1123
BstFNI CGCG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 912
BstHHI GCGC 3 cut(s) 232, 761, 911
BstKTI GATC 4 cut(s) 517, 735, 788, 1166
BstMAI GTCTC 3 cut(s) 526, 718, 747
BstMBI GATC 4 cut(s) 514, 732, 785, 1163
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 2 cut(s) 148, 157
BstNI CCWGG 1 cut(s) 527
BstSCI CCNGG 2 cut(s) 145, 525
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 2 cut(s) 447, 1093
BstV2I GAAGAC 2 cut(s) 285, 1149
BstX2I RGATCY 2 cut(s) 732, 785
BstYI RGATCY 2 cut(s) 732, 785
BstZ17I GTATAC 1 cut(s) 721
BstZI CGGCCG 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 876
BsuI GTATCC 1 cut(s) 43
BsuRI GGCC 3 cut(s) 79, 142, 872
BtrI CACGTC 1 cut(s) 1155
BtsCI GGATG 8 cut(s) 203, 485, 628, 873, 935, 963, 990, 1123
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 3 cut(s) 11, 862, 1101
CciNI GCGGCCGC 1 cut(s) 140
CfoI GCGC 3 cut(s) 232, 761, 911
Cfr13I GGNCC 2 cut(s) 77, 871
Csp6I GTAC 4 cut(s) 93, 691, 860, 933
CviAII CATG 3 cut(s) 327, 587, 899
CviQI GTAC 4 cut(s) 93, 691, 860, 933
DdeI CTNAG 3 cut(s) 572, 876, 1110
DpnI GATC 4 cut(s) 516, 734, 787, 1165
DpnII GATC 4 cut(s) 514, 732, 785, 1163
DraI TTTAAA 1 cut(s) 1119
EaeI YGGCCR 1 cut(s) 140
EagI CGGCCG 1 cut(s) 140
Eam1104I CTCTTC 2 cut(s) 234, 573
EarI CTCTTC 2 cut(s) 234, 573
EciI GGCGGA 1 cut(s) 492
EclXI CGGCCG 1 cut(s) 140
Eco130I CCWWGG 2 cut(s) 660, 850
Eco24I GRGCYC 3 cut(s) 44, 668, 1020
Eco52I CGGCCG 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 173
Eco81I CCTNAGG 1 cut(s) 876
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 2 cut(s) 660, 850
EcoT38I GRGCYC 3 cut(s) 44, 668, 1020
ErhI CCWWGG 2 cut(s) 660, 850
FaeI CATG 3 cut(s) 330, 590, 902
FaqI GGGAC 3 cut(s) 224, 236, 1083
FatI CATG 3 cut(s) 326, 586, 898
FauI CCCGC 2 cut(s) 67, 77
FbaI TGATCA 1 cut(s) 514
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 7 cut(s) 140, 143, 158, 161, 436, 439, 1107
FokI GGATG 8 cut(s) 210, 492, 635, 880, 942, 970, 997, 1110
FriOI GRGCYC 3 cut(s) 44, 668, 1020
Fsp4HI GCNGC 7 cut(s) 140, 143, 158, 161, 436, 439, 1107
FspBI CTAG 1 cut(s) 726
FspI TGCGCA 1 cut(s) 760
GlaI GCGC 3 cut(s) 231, 760, 910
GluI GCNGC 7 cut(s) 140, 143, 158, 161, 436, 439, 1107
HaeII RGCGCY 1 cut(s) 912
HaeIII GGCC 3 cut(s) 79, 142, 872
HapII CCGG 3 cut(s) 146, 182, 233
HhaI GCGC 3 cut(s) 232, 761, 911
Hin1II CATG 3 cut(s) 330, 590, 902
Hin6I GCGC 3 cut(s) 230, 759, 909
HinP1I GCGC 3 cut(s) 230, 759, 909
HincII GTYRAC 2 cut(s) 802, 951
HindII GTYRAC 2 cut(s) 802, 951
HindIII AAGCTT 3 cut(s) 389, 395, 794
HinfI GANTC 7 cut(s) 248, 574, 626, 704, 895, 922, 1034
HpaI GTTAAC 1 cut(s) 951
HpaII CCGG 3 cut(s) 146, 182, 233
HphI GGTGA 5 cut(s) 184, 196, 784, 1025, 1175
Hpy166II GTNNAC 5 cut(s) 693, 721, 802, 862, 951
Hpy188I TCNGA 4 cut(s) 125, 301, 472, 573
Hpy188III TCNNGA 5 cut(s) 647, 746, 977, 1031, 1161
Hpy8I GTNNAC 5 cut(s) 693, 721, 802, 862, 951
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 6 cut(s) 264, 363, 455, 490, 680, 1154
HpyCH4III ACNGT 5 cut(s) 15, 28, 859, 937, 1013
HpyCH4IV ACGT 1 cut(s) 1154
HpyCH4V TGCA 2 cut(s) 116, 265
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 157
HpyF3I CTNAG 3 cut(s) 572, 876, 1110
HpySE526I ACGT 1 cut(s) 1154
Hsp92II CATG 3 cut(s) 330, 590, 902
HspAI GCGC 3 cut(s) 230, 759, 909
Ksp22I TGATCA 1 cut(s) 514
KspAI GTTAAC 1 cut(s) 951
Kzo9I GATC 4 cut(s) 514, 732, 785, 1163
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 1 cut(s) 671
Lsp1109I GCAGC 2 cut(s) 447, 1093
LweI GCATC 2 cut(s) 188, 470
MaeI CTAG 1 cut(s) 726
MaeII ACGT 1 cut(s) 1154
MaeIII GTNAC 2 cut(s) 9, 655
MalI GATC 4 cut(s) 516, 734, 787, 1165
MbiI CCGCTC 1 cut(s) 84
MboI GATC 4 cut(s) 514, 732, 785, 1163
MflI RGATCY 2 cut(s) 732, 785
MhlI GDGCHC 3 cut(s) 44, 668, 1020
MluCI AATT 6 cut(s) 57, 126, 350, 409, 465, 1002
MlyI GAGTC 4 cut(s) 257, 583, 904, 931
MmeI TCCRAC 3 cut(s) 103, 234, 921
MnlI CCTC 7 cut(s) 168, 213, 237, 871, 1032, 1042, 1158
MroXI GAANNNNTTC 1 cut(s) 373
MseI TTAA 8 cut(s) 50, 129, 363, 393, 753, 950, 1118, 1198
MslI CAYNNNNRTG 1 cut(s) 903
MspA1I CMGCKG 1 cut(s) 438
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 3 cut(s) 146, 182, 233
MspR9I CCNGG 2 cut(s) 147, 527
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 1 cut(s) 527
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 2 cut(s) 148, 157
NciI CCSGG 1 cut(s) 147
NdeII GATC 4 cut(s) 514, 732, 785, 1163
NlaIII CATG 3 cut(s) 330, 590, 902
NlaIV GGNNCC 5 cut(s) 78, 667, 873, 963, 1029
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 1 cut(s) 9
NotI GCGGCCGC 1 cut(s) 140
NsbI TGCGCA 1 cut(s) 760
PciSI GCTCTTC 1 cut(s) 573
PctI GAATGC 1 cut(s) 781
PdmI GAANNNNTTC 1 cut(s) 373
PfeI GAWTC 3 cut(s) 626, 704, 1034
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 7 cut(s) 141, 144, 159, 162, 437, 440, 1108
PleI GAGTC 4 cut(s) 256, 582, 903, 930
PpsI GAGTC 4 cut(s) 256, 582, 903, 930
PshBI ATTAAT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 5 cut(s) 78, 667, 873, 963, 1029
PspPI GGNCC 2 cut(s) 77, 871
PsuI RGATCY 2 cut(s) 732, 785
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 4 cut(s) 94, 692, 861, 934
RsaNI GTAC 4 cut(s) 93, 691, 860, 933
RseI CAYNNNNRTG 1 cut(s) 903
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 8 cut(s) 50, 129, 363, 393, 753, 950, 1118, 1198
SatI GCNGC 7 cut(s) 140, 143, 158, 161, 436, 439, 1107
Sau3AI GATC 4 cut(s) 514, 732, 785, 1163
Sau96I GGNCC 2 cut(s) 77, 871
SchI GAGTC 4 cut(s) 257, 583, 904, 931
ScrFI CCNGG 2 cut(s) 147, 527
SduI GDGCHC 3 cut(s) 44, 668, 1020
SfaNI GCATC 2 cut(s) 188, 470
SmiMI CAYNNNNRTG 1 cut(s) 903
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 6 cut(s) 57, 126, 350, 409, 465, 1002
SsiI CCGC 8 cut(s) 74, 84, 139, 143, 158, 161, 438, 477
SspI AATATT 2 cut(s) 361, 766
SspMI CTAG 1 cut(s) 726
StyD4I CCNGG 2 cut(s) 145, 525
StyI CCWWGG 2 cut(s) 660, 850
TaaI ACNGT 5 cut(s) 15, 28, 859, 937, 1013
TaiI ACGT 1 cut(s) 1157
TaqI TCGA 3 cut(s) 167, 203, 978
TasI AATT 6 cut(s) 57, 126, 350, 409, 465, 1002
TatI WGTACW 4 cut(s) 92, 690, 859, 932
TauI GCSGC 5 cut(s) 142, 145, 160, 163, 441
TfiI GAWTC 3 cut(s) 626, 704, 1034
Tru1I TTAA 8 cut(s) 50, 129, 363, 393, 753, 950, 1118, 1198
Tru9I TTAA 8 cut(s) 50, 129, 363, 393, 753, 950, 1118, 1198
TscAI CASTG 3 cut(s) 18, 869, 1108
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 2 cut(s) 435, 1106
Tsp45I GTSAC 1 cut(s) 9
TspDTI ATGAA 5 cut(s) 318, 354, 474, 639, 845
TspGWI ACGGA 1 cut(s) 1055
TspRI CASTG 3 cut(s) 18, 869, 1108
Tth111I GACNNNGTC 1 cut(s) 7
Vha464I CTTAAG 1 cut(s) 392
VspI ATTAAT 1 cut(s) 363
XapI RAATTY 3 cut(s) 57, 350, 465
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 373
XspI CTAG 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.