MD15G1358300.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000243
Physical Location & Seq
Reverse (-)
43188889 .. 43195101
6213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1358300.v1.1.491

Sequence Viewer

Length: 1746 bp
ATGCATCACATACCTCGATTATTCTCTCATAGCAAGGGAACGAAAGCTATGGATCCGAAGAAGAACCGCCGGAAGCCGAGGCTCGAGCGCCGCAATGCGGTGAAGCACATTGACTACGACGCTGCCTCGACGTCGTCCTCACTCGATGACTCGTCGTCGTCGGCGTCGCTCTACACGCGCTCGTTCGACCTGGACCGGACGAGCTTCCGGGTCGAGGGCAACGAGGGCGATATGGAGCGGATATACAAGACCTTGGGCCTCGGACCCGACGACTTCTCGATATCGGAGGAGGACTGGGTGGCCAGGAAGATCCGCTCGTCTTCGGACCGTCTTCCCATCTCCAGATTTTACGGGTTGGATAGTCCGGATAGTCCGAAACCCGACGTATTGAGGGAAGAAGTGGCTGAGTTGTCCGATAGAGTTAGGGCTGTTACTGTTGCTGCAACTGAGTTGACTCGGGCTGACTCGGCCGTTCCAAGCGGGTTTTATAATGTTGCAGCTGCTAATTCTGTTTCAGCTGGGGTTTGTGTTGGGATCAAGGGTGTGCGGCCGCCGGTGCTCAAGCCGCCGCCGTCGATGAGGGTTCCGGTGATCGACGACGGATGCTCGACTTGGGACATTCTGAGGGATTTCGCTCCAGAAGGCGCAGGAAACCCTGTGGGAAAGAGGATTGATTCTTCTTCTGCAGCTTCCTCTTCTGATGAAAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGAGAGGTTGGAGAGGAGGGTGCTGTGGAGATTGAGATTAGATATACTGTGCCGGTCTCTGAAGGGTGTTCATTTACGACGTCGAATGATGATGATTCTTCCAGCACCACCACTGTTTCTCCGAATGAGAGAATTTCGCCGAACGGGAGGCTGAAGCTTATTATCACTCGCTGGGAGAAGGGTGACCTTCTTGGCAGTGGGTCTTTCGGGTCGGTGTATGAAGCAATTTCTGATGGCGGATGCTTCATTGCTGTCAAGGAAGTTTCATTGCTTGACAAAGGAAATCAGGGAAAGCAAAGAGTTTATCAACTTGAACAGGAGATTGCTCTTTTGAGTCAGTTTGAACATCATAACATAGTTCAATATTATGGCACCCAAAAGGATGAATCAAACCTTTATATCTTTCTGGAGCTTGTAGCTAAAGGTTCCCTTCAAAAACTCTATCAGACATATCATCTTACAGATTCTCATGTATCTGAATACACAAGACAGATCCTGCATGGACTGAAGTATCTTCATGACCGAAAAGTGGTTCACAGGGACGTTAAATGTGCAAACATTTTGGTGCATGCAAATGGATCTGTGAAGCTTGCAGATTTTGGATTGGCAAAAACTATCCAAAATATGAATGACATTAAATCTTGCCAGGGGACTGCATTCTGGATGGCCCCAGAGGTTGTTAACCGAAAGAGCCAAGGTTATGGGCTTCCAGCTGATATATGGAGCCTTGGATGCACTGTGTTGGAGATGTTAACTGGGCGGGTTCCATACTCTAATCTTGAATGGATGCAAGCGTTATTTAAGATTGGTAAGGGGGAGCCCCCTCAGGTTCCTGATTCTCTTTCCAAGGATGCACAAGATTTCATCCATCAATGCCTACAAGTTGATCCAAAAAAACGTCCCACTGCTGCTCAGCTCTTAATTCATCCATTTCTTAAGAGACCTCTTCCCACGTCTTCTGGATCAGCATCTCCTTATCATCACCGGGGGCCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

582

Amino Acids

64.3

Weight (kDa)

5.56

Isoelectric Point (pI)

50.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 308 - 561 6.7e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 310 - 555 2.4e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AatII GACGTC 2 cut(s) 134, 830
AccB1I GGYRCC 1 cut(s) 1118
AccBSI CCGCTC 2 cut(s) 238, 315
AccII CGCG 1 cut(s) 178
AccIII TCCGGA 1 cut(s) 364
AclWI GGATC 8 cut(s) 47, 60, 304, 542, 1234, 1333, 1628, 1717
AcoI YGGCCR 3 cut(s) 300, 468, 548
AcsI RAATTY 1 cut(s) 879
AcuI CTGAAG 3 cut(s) 828, 920, 1274
AcyI GRCGYC 3 cut(s) 131, 164, 827
AfiI CCNNNNNNNGG 3 cut(s) 97, 214, 1276
AflII CTTAAG 1 cut(s) 1682
AgsI TTSAA 5 cut(s) 1061, 1091, 1109, 1181, 1529
AhdI GACNNNNNGTC 1 cut(s) 154
AjiI CACGTC 1 cut(s) 1701
AjnI CCWGG 3 cut(s) 189, 302, 1392
AjuI GAANNNNNNNTTGG 2 cut(s) 738, 770
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 2 cut(s) 808, 1681
AlwI GGATC 8 cut(s) 47, 60, 304, 542, 1234, 1333, 1628, 1717
AlwNI CAGNNNCTG 1 cut(s) 1243
Ama87I CYCGRG 2 cut(s) 83, 456
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 6 cut(s) 256, 300, 468, 548, 1413, 1736
ApaI GGGCCC 1 cut(s) 1740
ApeKI GCWGC 6 cut(s) 122, 440, 497, 500, 686, 1655
ApoI RAATTY 1 cut(s) 879
AspLEI GCGC 3 cut(s) 90, 180, 647
AspS9I GGNCC 7 cut(s) 193, 256, 263, 325, 1414, 1736, 1737
AsuC2I CCSGG 2 cut(s) 209, 1733
AsuHPI GGTGA 4 cut(s) 112, 601, 941, 1721
AvaI CYCGRG 2 cut(s) 83, 456
AvaII GGWCC 3 cut(s) 193, 263, 325
AxyI CCTNAGG 1 cut(s) 1572
BaeGI GKGCMC 1 cut(s) 1740
BaeI ACNNNNGTAYC 2 cut(s) 1241, 1274
BalI TGGCCA 1 cut(s) 302
BamHI GGATCC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 1118
BanII GRGCYC 2 cut(s) 1569, 1740
BbsI GAAGAC 3 cut(s) 312, 323, 1695
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 6 cut(s) 109, 427, 487, 509, 698, 1642
BccI CCATC 4 cut(s) 344, 974, 1405, 1623
BceAI ACGGC 2 cut(s) 455, 556
BciT130I CCWGG 3 cut(s) 191, 304, 1394
BcnI CCSGG 2 cut(s) 209, 1733
BcoDI GTCTC 2 cut(s) 808, 1681
BfmI CTRYAG 1 cut(s) 684
BfoI RGCGCY 1 cut(s) 91
BfrI CTTAAG 1 cut(s) 1682
BlpI GCTNAGC 1 cut(s) 1659
Bme1390I CCNGG 5 cut(s) 191, 209, 304, 1394, 1733
Bme18I GGWCC 3 cut(s) 193, 263, 325
BmeRI GACNNNNNGTC 1 cut(s) 154
BmeT110I CYCGRG 2 cut(s) 83, 456
BmgBI CACGTC 1 cut(s) 1701
BmgT120I GGNCC 7 cut(s) 193, 256, 263, 325, 1414, 1736, 1737
BmrFI CCNGG 5 cut(s) 191, 209, 304, 1394, 1733
BmrI ACTGGG 2 cut(s) 304, 1512
BmsI GCATC 7 cut(s) 13, 593, 977, 1469, 1524, 1588, 1724
BmuI ACTGGG 2 cut(s) 304, 1512
BpiI GAAGAC 3 cut(s) 312, 323, 1695
BpmI CTGGAG 3 cut(s) 325, 621, 1175
Bpu1102I GCTNAGC 1 cut(s) 1659
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 2 cut(s) 209, 1733
BsaBI GATNNNNATC 1 cut(s) 1714
BsaHI GRCGYC 3 cut(s) 131, 164, 827
BsaI GGTCTC 2 cut(s) 808, 1681
BsaJI CCNNGG 8 cut(s) 77, 252, 259, 1393, 1441, 1474, 1593, 1732
BsaWI WCCGGW 3 cut(s) 195, 364, 586
BsaXI ACNNNNNCTCC 2 cut(s) 850, 880
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 214, 1276
Bse118I RCCGGY 2 cut(s) 553, 799
Bse1I ACTGG 2 cut(s) 299, 1507
Bse21I CCTNAGG 1 cut(s) 1572
Bse3DI GCAATG 3 cut(s) 100, 993, 1013
Bse8I GATNNNNATC 1 cut(s) 1714
BseAI TCCGGA 1 cut(s) 364
BseBI CCWGG 3 cut(s) 191, 304, 1394
BseDI CCNNGG 8 cut(s) 77, 252, 259, 1393, 1441, 1474, 1593, 1732
BseGI GGATG 9 cut(s) 608, 992, 1135, 1416, 1484, 1539, 1603, 1611, 1672
BseJI GATNNNNATC 1 cut(s) 1714
BseLI CCNNNNNNNGG 3 cut(s) 97, 214, 1276
BseMI GCAATG 3 cut(s) 100, 993, 1013
BseMII CTCAG 5 cut(s) 396, 438, 614, 1586, 1673
BseNI ACTGG 2 cut(s) 299, 1507
BseRI GAGGAG 2 cut(s) 302, 776
BseSI GKGCMC 1 cut(s) 1740
BseX3I CGGCCG 2 cut(s) 468, 548
BseXI GCAGC 6 cut(s) 109, 427, 487, 509, 698, 1642
BseYI CCCAGC 3 cut(s) 518, 918, 1739
Bsh1236I CGCG 1 cut(s) 178
Bsh1285I CGRYCG 2 cut(s) 471, 551
BshFI GGCC 6 cut(s) 258, 302, 470, 550, 1415, 1738
BshNI GGYRCC 1 cut(s) 1118
BsiEI CGRYCG 2 cut(s) 471, 551
BsiHKAI GWGCWC 1 cut(s) 561
BsiHKCI CYCGRG 2 cut(s) 83, 456
BsiSI CCGG 8 cut(s) 70, 196, 208, 365, 554, 587, 800, 1732
BslFI GGGAC 4 cut(s) 629, 1301, 1411, 1632
BslI CCNNNNNNNGG 3 cut(s) 97, 214, 1276
BsmAI GTCTC 2 cut(s) 808, 1681
BsmFI GGGAC 4 cut(s) 629, 1301, 1411, 1632
BsmI GAATGC 1 cut(s) 1403
BsnI GGCC 6 cut(s) 258, 302, 470, 550, 1415, 1738
Bso31I GGTCTC 2 cut(s) 808, 1681
BsoBI CYCGRG 2 cut(s) 83, 456
Bsp120I GGGCCC 1 cut(s) 1736
Bsp1286I GDGCHC 3 cut(s) 561, 1569, 1740
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 8 cut(s) 52, 309, 534, 591, 1239, 1325, 1633, 1709
Bsp1720I GCTNAGC 1 cut(s) 1659
BspANI GGCC 6 cut(s) 258, 302, 470, 550, 1415, 1738
BspCNI CTCAG 5 cut(s) 397, 439, 615, 1585, 1672
BspEI TCCGGA 1 cut(s) 364
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 1 cut(s) 1264
BspMAI CTGCAG 1 cut(s) 688
BspPI GGATC 8 cut(s) 47, 60, 304, 542, 1234, 1333, 1628, 1717
BspT107I GGYRCC 1 cut(s) 1118
BspTI CTTAAG 1 cut(s) 1682
BspTNI GGTCTC 2 cut(s) 808, 1681
BsrBI CCGCTC 2 cut(s) 238, 315
BsrDI GCAATG 3 cut(s) 100, 993, 1013
BsrFI RCCGGY 2 cut(s) 553, 799
BsrI ACTGG 2 cut(s) 299, 1507
BssAI RCCGGY 2 cut(s) 553, 799
BssECI CCNNGG 8 cut(s) 77, 252, 259, 1393, 1441, 1474, 1593, 1732
BssMI GATC 8 cut(s) 52, 309, 534, 591, 1239, 1325, 1633, 1709
BssNI GRCGYC 3 cut(s) 131, 164, 827
BssT1I CCWWGG 4 cut(s) 252, 1441, 1474, 1593
Bst2UI CCWGG 3 cut(s) 191, 304, 1394
Bst4CI ACNGT 5 cut(s) 329, 436, 796, 862, 1486
Bst6I CTCTTC 2 cut(s) 700, 1698
BstACI GRCGYC 3 cut(s) 131, 164, 827
BstAFI CTTAAG 1 cut(s) 1682
BstC8I GCNNGC 3 cut(s) 1317, 1338, 1539
BstDEI CTNAG 5 cut(s) 405, 447, 623, 1572, 1659
BstEII GGTNACC 1 cut(s) 929
BstF5I GGATG 9 cut(s) 608, 992, 1135, 1416, 1484, 1539, 1603, 1611, 1672
BstFNI CGCG 1 cut(s) 178
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 3 cut(s) 90, 180, 647
BstKTI GATC 8 cut(s) 55, 312, 537, 594, 1242, 1328, 1636, 1712
BstMAI GTCTC 2 cut(s) 808, 1681
BstMBI GATC 8 cut(s) 52, 309, 534, 591, 1239, 1325, 1633, 1709
BstMCI CGRYCG 2 cut(s) 471, 551
BstMWI GCNNNNNNNGC 6 cut(s) 175, 225, 467, 556, 565, 1479
BstNI CCWGG 3 cut(s) 191, 304, 1394
BstNSI RCATGY 1 cut(s) 1319
BstPI GGTNACC 1 cut(s) 929
BstSCI CCNGG 5 cut(s) 189, 207, 302, 1392, 1731
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 1 cut(s) 1740
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 6 cut(s) 109, 427, 487, 509, 698, 1642
BstV2I GAAGAC 3 cut(s) 312, 323, 1695
BstX2I RGATCY 4 cut(s) 52, 309, 1239, 1325
BstXI CCANNNNNNTGG 1 cut(s) 1448
BstYI RGATCY 4 cut(s) 52, 309, 1239, 1325
BstZI CGGCCG 2 cut(s) 468, 548
Bsu36I CCTNAGG 1 cut(s) 1572
BsuRI GGCC 6 cut(s) 258, 302, 470, 550, 1415, 1738
BtrI CACGTC 1 cut(s) 1701
BtsCI GGATG 9 cut(s) 608, 992, 1135, 1416, 1484, 1539, 1603, 1611, 1672
BtsI GCAGTG 2 cut(s) 949, 1650
BtsIMutI CAGTG 4 cut(s) 858, 949, 1482, 1650
Cac8I GCNNGC 3 cut(s) 1317, 1338, 1539
CaiI CAGNNNCTG 1 cut(s) 1243
CciI TCATGA 1 cut(s) 1264
CciNI GCGGCCGC 1 cut(s) 548
CfoI GCGC 3 cut(s) 90, 180, 647
Cfr10I RCCGGY 2 cut(s) 553, 799
Cfr13I GGNCC 7 cut(s) 193, 256, 263, 325, 1414, 1736, 1737
CpoI CGGWCCG 1 cut(s) 325
CseI GACGC 2 cut(s) 128, 153
CspI CGGWCCG 1 cut(s) 325
CviAII CATG 4 cut(s) 1217, 1247, 1265, 1316
DdeI CTNAG 5 cut(s) 405, 447, 623, 1572, 1659
DpnI GATC 8 cut(s) 54, 311, 536, 593, 1241, 1327, 1635, 1711
DpnII GATC 8 cut(s) 52, 309, 534, 591, 1239, 1325, 1633, 1709
DriI GACNNNNNGTC 1 cut(s) 154
EaeI YGGCCR 3 cut(s) 300, 468, 548
EagI CGGCCG 2 cut(s) 468, 548
Eam1104I CTCTTC 2 cut(s) 700, 1698
Eam1105I GACNNNNNGTC 1 cut(s) 154
EarI CTCTTC 2 cut(s) 700, 1698
EciI GGCGGA 1 cut(s) 999
EclXI CGGCCG 2 cut(s) 468, 548
Eco130I CCWWGG 4 cut(s) 252, 1441, 1474, 1593
Eco24I GRGCYC 2 cut(s) 1569, 1740
Eco31I GGTCTC 2 cut(s) 808, 1681
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 3 cut(s) 193, 263, 325
Eco52I CGGCCG 2 cut(s) 468, 548
Eco57I CTGAAG 3 cut(s) 828, 920, 1274
Eco81I CCTNAGG 1 cut(s) 1572
Eco88I CYCGRG 2 cut(s) 83, 456
Eco91I GGTNACC 1 cut(s) 929
EcoO109I RGGNCCY 1 cut(s) 1736
EcoO65I GGTNACC 1 cut(s) 929
EcoRII CCWGG 3 cut(s) 189, 302, 1392
EcoRV GATATC 1 cut(s) 282
EcoT14I CCWWGG 4 cut(s) 252, 1441, 1474, 1593
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 1569, 1740
ErhI CCWWGG 4 cut(s) 252, 1441, 1474, 1593
FaeI CATG 4 cut(s) 1220, 1250, 1268, 1319
FalI AAGNNNNNCTT 2 cut(s) 1161, 1193
FaqI GGGAC 4 cut(s) 629, 1301, 1411, 1632
FatI CATG 4 cut(s) 1216, 1246, 1264, 1315
FauI CCCGC 2 cut(s) 473, 1500
FokI GGATG 9 cut(s) 615, 999, 1142, 1423, 1491, 1546, 1598, 1610, 1659
FriOI GRGCYC 2 cut(s) 1569, 1740
GlaI GCGC 3 cut(s) 89, 179, 646
GsaI CCCAGC 3 cut(s) 522, 922, 1743
GsuI CTGGAG 3 cut(s) 325, 621, 1175
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 6 cut(s) 258, 302, 470, 550, 1415, 1738
HapII CCGG 8 cut(s) 70, 196, 208, 365, 554, 587, 800, 1732
HgaI GACGC 2 cut(s) 128, 153
HhaI GCGC 3 cut(s) 90, 180, 647
Hin1I GRCGYC 3 cut(s) 131, 164, 827
Hin1II CATG 4 cut(s) 1220, 1250, 1268, 1319
Hin6I GCGC 3 cut(s) 88, 178, 645
HinP1I GCGC 3 cut(s) 88, 178, 645
HincII GTYRAC 3 cut(s) 453, 1429, 1500
HindII GTYRAC 3 cut(s) 453, 1429, 1500
HindIII AAGCTT 2 cut(s) 902, 1334
HinfI GANTC 9 cut(s) 149, 454, 464, 674, 842, 1081, 1133, 1211, 1583
HpaI GTTAAC 2 cut(s) 1429, 1500
HpaII CCGG 8 cut(s) 70, 196, 208, 365, 554, 587, 800, 1732
HphI GGTGA 4 cut(s) 112, 601, 941, 1721
Hpy166II GTNNAC 4 cut(s) 453, 1282, 1429, 1500
Hpy8I GTNNAC 4 cut(s) 453, 1282, 1429, 1500
HpyAV CCTTC 6 cut(s) 635, 740, 803, 919, 944, 1187
HpyCH4III ACNGT 5 cut(s) 329, 436, 796, 862, 1486
HpyCH4IV ACGT 6 cut(s) 131, 384, 827, 1290, 1645, 1700
HpyF10VI GCNNNNNNNGC 6 cut(s) 175, 225, 467, 556, 565, 1479
HpyF3I CTNAG 5 cut(s) 405, 447, 623, 1572, 1659
HpySE526I ACGT 6 cut(s) 131, 384, 827, 1290, 1645, 1700
Hsp92I GRCGYC 3 cut(s) 131, 164, 827
Hsp92II CATG 4 cut(s) 1220, 1250, 1268, 1319
HspAI GCGC 3 cut(s) 88, 178, 645
Kpn2I TCCGGA 1 cut(s) 364
KspAI GTTAAC 2 cut(s) 1429, 1500
Kzo9I GATC 8 cut(s) 52, 309, 534, 591, 1239, 1325, 1633, 1709
LmnI GCTCC 5 cut(s) 235, 640, 1156, 1470, 1564
Lsp1109I GCAGC 6 cut(s) 109, 427, 487, 509, 698, 1642
LweI GCATC 7 cut(s) 13, 593, 977, 1469, 1524, 1588, 1724
MaeII ACGT 6 cut(s) 131, 384, 827, 1290, 1645, 1700
MaeIII GTNAC 2 cut(s) 430, 929
MalI GATC 8 cut(s) 54, 311, 536, 593, 1241, 1327, 1635, 1711
MbiI CCGCTC 2 cut(s) 238, 315
MboI GATC 8 cut(s) 52, 309, 534, 591, 1239, 1325, 1633, 1709
MflI RGATCY 4 cut(s) 52, 309, 1239, 1325
MhlI GDGCHC 3 cut(s) 561, 1569, 1740
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 4 cut(s) 505, 879, 972, 1668
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 4 cut(s) 143, 448, 458, 1090
MmeI TCCRAC 3 cut(s) 336, 736, 1470
Mox20I TGGCCA 1 cut(s) 302
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 364
MscI TGGCCA 1 cut(s) 302
MseI TTAA 7 cut(s) 1293, 1383, 1428, 1499, 1548, 1667, 1683
MslI CAYNNNNRTG 2 cut(s) 1310, 1320
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 4 cut(s) 500, 518, 1460, 1743
MspCI CTTAAG 1 cut(s) 1682
MspI CCGG 8 cut(s) 70, 196, 208, 365, 554, 587, 800, 1732
MspR9I CCNGG 5 cut(s) 191, 209, 304, 1394, 1733
Mva1269I GAATGC 1 cut(s) 1403
MvaI CCWGG 3 cut(s) 191, 304, 1394
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 6 cut(s) 175, 225, 467, 556, 565, 1479
NciI CCSGG 2 cut(s) 209, 1733
NdeII GATC 8 cut(s) 52, 309, 534, 591, 1239, 1325, 1633, 1709
NlaIII CATG 4 cut(s) 1220, 1250, 1268, 1319
NmeAIII GCCGAG 2 cut(s) 102, 446
NmuCI GTSAC 1 cut(s) 929
NotI GCGGCCGC 1 cut(s) 548
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 1319
PaeI GCATGC 1 cut(s) 1319
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 1264
PcsI WCGNNNNNNNCGW 3 cut(s) 161, 219, 267
PctI GAATGC 1 cut(s) 1403
PfeI GAWTC 5 cut(s) 674, 842, 1133, 1211, 1583
PflFI GACNNNGTC 1 cut(s) 133
PleI GAGTC 4 cut(s) 143, 448, 458, 1089
PpsI GAGTC 4 cut(s) 143, 448, 458, 1089
PsiI TTATAA 1 cut(s) 489
Psp6I CCWGG 3 cut(s) 189, 302, 1392
PspEI GGTNACC 1 cut(s) 929
PspFI CCCAGC 3 cut(s) 518, 918, 1739
PspGI CCWGG 3 cut(s) 189, 302, 1392
PspOMI GGGCCC 1 cut(s) 1736
PspPI GGNCC 7 cut(s) 193, 256, 263, 325, 1414, 1736, 1737
PspXI VCTCGAGB 1 cut(s) 83
PstI CTGCAG 1 cut(s) 688
PstNI CAGNNNCTG 1 cut(s) 1243
PsuI RGATCY 4 cut(s) 52, 309, 1239, 1325
PsyI GACNNNGTC 1 cut(s) 133
PvuII CAGCTG 4 cut(s) 500, 518, 1460, 1743
RseI CAYNNNNRTG 2 cut(s) 1310, 1320
Rsr2I CGGWCCG 1 cut(s) 325
RsrII CGGWCCG 1 cut(s) 325
SaqAI TTAA 7 cut(s) 1293, 1383, 1428, 1499, 1548, 1667, 1683
Sau3AI GATC 8 cut(s) 52, 309, 534, 591, 1239, 1325, 1633, 1709
Sau96I GGNCC 7 cut(s) 193, 256, 263, 325, 1414, 1736, 1737
SchI GAGTC 4 cut(s) 143, 448, 458, 1090
ScrFI CCNGG 5 cut(s) 191, 209, 304, 1394, 1733
SduI GDGCHC 3 cut(s) 561, 1569, 1740
SfaNI GCATC 7 cut(s) 13, 593, 977, 1469, 1524, 1588, 1724
SfcI CTRYAG 1 cut(s) 684
Sfr274I CTCGAG 1 cut(s) 83
SgrAI CRCCGGYG 1 cut(s) 553
SinI GGWCC 3 cut(s) 193, 263, 325
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 2 cut(s) 1310, 1320
SmlI CTYRAG 3 cut(s) 83, 560, 1682
SmoI CTYRAG 3 cut(s) 83, 560, 1682
SphI GCATGC 1 cut(s) 1319
Sse9I AATT 4 cut(s) 505, 879, 972, 1668
SspI AATATT 1 cut(s) 1112
StyD4I CCNGG 5 cut(s) 189, 207, 302, 1392, 1731
StyI CCWWGG 4 cut(s) 252, 1441, 1474, 1593
TaaI ACNGT 5 cut(s) 329, 436, 796, 862, 1486
TaiI ACGT 6 cut(s) 134, 387, 830, 1293, 1648, 1703
TaqII GACCGA 1 cut(s) 1284
TasI AATT 4 cut(s) 505, 879, 972, 1668
TauI GCSGC 5 cut(s) 93, 550, 553, 568, 571
TfiI GAWTC 5 cut(s) 674, 842, 1133, 1211, 1583
Tru1I TTAA 7 cut(s) 1293, 1383, 1428, 1499, 1548, 1667, 1683
Tru9I TTAA 7 cut(s) 1293, 1383, 1428, 1499, 1548, 1667, 1683
TscAI CASTG 4 cut(s) 865, 949, 1489, 1657
TseFI GTSAC 1 cut(s) 929
TseI GCWGC 6 cut(s) 122, 440, 497, 500, 686, 1655
Tsp45I GTSAC 1 cut(s) 929
TspGWI ACGGA 1 cut(s) 615
TspRI CASTG 4 cut(s) 865, 949, 1489, 1657
Tth111I GACNNNGTC 1 cut(s) 133
Vha464I CTTAAG 1 cut(s) 1682
VpaK11BI GGWCC 3 cut(s) 193, 263, 325
XapI RAATTY 1 cut(s) 879
XceI RCATGY 1 cut(s) 1319
XcmI CCANNNNNNNNNTGG 1 cut(s) 1464
XhoI CTCGAG 1 cut(s) 83
ZraI GACGTC 2 cut(s) 132, 828
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.