pycom08g14750

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000243
Physical Location & Seq
Reverse (-)
14145067 .. 14149915
4849 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g14750.1

Sequence Viewer

Length: 1725 bp
ATGCATCACATACCTCGATTTTTCTCTCATAGTAAGGGGACGAAAGCCATGGATCCGAAGAAGAACCGCCGGAAGCCGAGGCTCGAGCGCCGTAATGCGGGGAAGCACTTTGACTACGACGCCGCCTCGATGTCATCCTCACTCGACGACTCCTCGTCGTCGGCCTCACTCCACACCCGCTCGTTCGACTTGGACCGGACGAGCTTCCGGGTCGAGGGGAACGAGGGCGATATGGAGCGGATATACAAGACCTTGGGCCTCGGACCCGACGACTTCTCGATATCGGAGGAGGATTGGGTGGCCCGGAAGATCCGCTCGACTTCGGATGTTCTCCCCATATCCAGATTGTACGGGTTGGATAGTCTAGATAGTCCGAAACCCGACGAATTGAGGGAAGAAGTGGCTGAGTTGTCCGATAGAGTAAGGGCTGTTACTGTTGCTGCAACTGAGTTGACTCTAGCTGACTCGCCGGTTCCAAGCGGGGGTTATAATGCTGATGCGGCTGCTGCTGCTAGTTCTGTTTCAGCTGGGGTTTCTATTGGGATTAAGGGTGTGCGGCCGCCCGTGCTCAGGCCGCCGCCGTTGATGAGGGTTCCGGTGATTGACGACGGATGCTCGACTTGGGACATTCTGAGGGATTTCGCGCCAGAAGGCGCAGGAAATGCGGTGGGAAAGAGGCTTGGTTCTTCTTCGGCAGCTTCCTCTTGTTCTGATGAAGAAGAAGAAGGAGAGGTTGGAGAGGAGGGCGCTGTGGAAGGTGAGATTAGAGAGACTGTGCCGGTCTCTGAAGGGTGTTCATTTACGACGTCGAATGATGATGATTCTTCTAGCACCACCACTGTTTCTCCCAATGAGAGAATTTCGCCGAATGGGAGGCTAAATCTTATTATCACTCACTGGGAGAAGGGTGACCTTCTTGGTAGTGGGTCTTTCGGGTCGGTATATGAAGCCATTTCTGATGGTGGAAGCTTCATTGCTGTGAAGGAAGTTTCATTGCTTGATAAAGGAAGTCAGGGGAGGCAAAGAGTTTATCAACTTGAACAGGAGATTGCTCTTTTGAGTCAGTTTGAACATCATAACATAGTTCAATATTACGGCACCCAAAGGGATGAATCAAACCTTTATATCTTTCTGGAGCTTGTAGCTAAAGGTTCCCTTCAGAAACTCTATCAGACGTATCATCTTACAGATTCTCATGTATCTGAATACACAAGATCGATCCTGCATGGACTGAAGTATCTTCATGACCGAAAAGTGGTTCACAGGGACATCAAATGTGCAAACATTCTGGTGCATGCAAATGGATCTGTGAAGCTTGCAGATTTTGGATTGGCAAAGACTATCCAAAATATGAATGACATTAAATCTTGCCAGGGGACTGCATTCTGGATGGCCCCAGAGGTTGTTAACCGAAAGAGCCAAGGTTATGGGCTTCCAGCTGATATATGGAGCCTTGGATGCACTGTGTTGGAGATGTTAACTGGGCGGGTTCCATACTCTAATCTTGAATGGATGCAGGCATTATTTAAGATTGGAAAGGGAGAGCCCCCTCAGGTTCCCGATTCTCTTTCAAAAGATGCACAAGATTTCATCGATCGGTGCCTACAAGTTGATCCAAACAAACGCCCCTCTGCTGCTCTGCTCTTAACTCATCCATTTCTTAAGAGACCCCTTCCCACGTCTTCTGGATCAGCATCTCCATATTATCAGCGGGGGCCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

575

Amino Acids

62.87

Weight (kDa)

5.61

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 301 - 554 2.6e-71 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 303 - 547 2.2e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AatII GACGTC 1 cut(s) 809
AccB1I GGYRCC 2 cut(s) 1097, 1599
AccBSI CCGCTC 3 cut(s) 180, 238, 315
AccII CGCG 1 cut(s) 644
AclWI GGATC 7 cut(s) 47, 60, 304, 1213, 1312, 1607, 1696
AcoI YGGCCR 1 cut(s) 557
AcsI RAATTY 1 cut(s) 858
AcuI CTGAAG 3 cut(s) 807, 1142, 1253
AcyI GRCGYC 2 cut(s) 120, 806
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 5 cut(s) 97, 214, 482, 570, 1255
AflII CTTAAG 1 cut(s) 1661
AgsI TTSAA 5 cut(s) 1040, 1070, 1088, 1508, 1572
AhdI GACNNNNNGTC 1 cut(s) 154
AjiI CACGTC 1 cut(s) 1680
AjnI CCWGG 1 cut(s) 1371
AjuI GAANNNNNNNTTGG 2 cut(s) 717, 749
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 3 cut(s) 764, 787, 1660
AlwI GGATC 7 cut(s) 47, 60, 304, 1213, 1312, 1607, 1696
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 7 cut(s) 162, 256, 300, 557, 572, 1392, 1715
ApaI GGGCCC 1 cut(s) 1719
ApeKI GCWGC 6 cut(s) 440, 503, 506, 509, 695, 1634
ApoI RAATTY 1 cut(s) 858
AspLEI GCGC 4 cut(s) 90, 646, 656, 749
AspS9I GGNCC 7 cut(s) 193, 256, 263, 301, 1393, 1715, 1716
AsuC2I CCSGG 2 cut(s) 209, 304
AsuHPI GGTGA 3 cut(s) 610, 770, 920
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 2 cut(s) 193, 263
AxyI CCTNAGG 1 cut(s) 1551
BaeGI GKGCMC 1 cut(s) 1719
BaeI ACNNNNGTAYC 2 cut(s) 1220, 1253
BamHI GGATCC 1 cut(s) 52
BanI GGYRCC 2 cut(s) 1097, 1599
BanII GRGCYC 2 cut(s) 1548, 1719
BbsI GAAGAC 1 cut(s) 1674
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 6 cut(s) 427, 490, 493, 496, 707, 1621
BccI CCATC 2 cut(s) 953, 1384
BceAI ACGGC 3 cut(s) 75, 565, 1111
BciT130I CCWGG 1 cut(s) 1373
BcnI CCSGG 2 cut(s) 209, 304
BcoDI GTCTC 3 cut(s) 764, 787, 1660
BfaI CTAG 4 cut(s) 365, 458, 513, 828
BfoI RGCGCY 2 cut(s) 91, 750
BfrI CTTAAG 1 cut(s) 1661
Bme1390I CCNGG 3 cut(s) 209, 304, 1373
Bme18I GGWCC 2 cut(s) 193, 263
BmeRI GACNNNNNGTC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 83
BmgBI CACGTC 1 cut(s) 1680
BmgT120I GGNCC 7 cut(s) 193, 256, 263, 301, 1393, 1715, 1716
BmrFI CCNGG 3 cut(s) 209, 304, 1373
BmrI ACTGGG 2 cut(s) 907, 1491
BmsI GCATC 7 cut(s) 13, 487, 602, 1448, 1503, 1567, 1703
BmuI ACTGGG 2 cut(s) 907, 1491
BpiI GAAGAC 1 cut(s) 1674
BpmI CTGGAG 1 cut(s) 1154
Bpu10I CCTNAGC 1 cut(s) 569
BpuMI CCSGG 2 cut(s) 209, 304
Bsa29I ATCGAT 2 cut(s) 1217, 1593
BsaBI GATNNNNATC 1 cut(s) 1693
BsaHI GRCGYC 2 cut(s) 120, 806
BsaI GGTCTC 2 cut(s) 787, 1660
BsaJI CCNNGG 7 cut(s) 48, 77, 252, 259, 1372, 1420, 1453
BsaWI WCCGGW 2 cut(s) 195, 595
BsaXI ACNNNNNCTCC 2 cut(s) 829, 859
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 214, 482, 570, 1255
Bse118I RCCGGY 2 cut(s) 469, 778
Bse1I ACTGG 2 cut(s) 902, 1486
Bse21I CCTNAGG 1 cut(s) 1551
Bse3DI GCAATG 2 cut(s) 972, 992
Bse8I GATNNNNATC 1 cut(s) 1693
BseBI CCWGG 1 cut(s) 1373
BseCI ATCGAT 2 cut(s) 1217, 1593
BseDI CCNNGG 7 cut(s) 48, 77, 252, 259, 1372, 1420, 1453
BseGI GGATG 8 cut(s) 134, 331, 617, 1114, 1395, 1463, 1518, 1651
BseJI GATNNNNATC 1 cut(s) 1693
BseLI CCNNNNNNNGG 5 cut(s) 97, 214, 482, 570, 1255
BseMI GCAATG 2 cut(s) 972, 992
BseMII CTCAG 5 cut(s) 396, 438, 583, 623, 1565
BseNI ACTGG 2 cut(s) 902, 1486
BseRI GAGGAG 3 cut(s) 142, 302, 755
BseSI GKGCMC 1 cut(s) 1719
BseX3I CGGCCG 1 cut(s) 557
BseXI GCAGC 6 cut(s) 427, 490, 493, 496, 707, 1621
BseYI CCCAGC 2 cut(s) 527, 1718
Bsh1236I CGCG 1 cut(s) 644
Bsh1285I CGRYCG 2 cut(s) 560, 1597
BshFI GGCC 7 cut(s) 164, 258, 302, 559, 574, 1394, 1717
BshNI GGYRCC 2 cut(s) 1097, 1599
BshVI ATCGAT 2 cut(s) 1217, 1593
BsiEI CGRYCG 2 cut(s) 560, 1597
BsiHKAI GWGCWC 1 cut(s) 570
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 7 cut(s) 70, 196, 208, 304, 470, 596, 779
BslFI GGGAC 4 cut(s) 52, 638, 1280, 1390
BslI CCNNNNNNNGG 5 cut(s) 97, 214, 482, 570, 1255
BsmAI GTCTC 3 cut(s) 764, 787, 1660
BsmFI GGGAC 4 cut(s) 52, 638, 1280, 1390
BsmI GAATGC 1 cut(s) 1382
BsnI GGCC 7 cut(s) 164, 258, 302, 559, 574, 1394, 1717
Bso31I GGTCTC 2 cut(s) 787, 1660
BsoBI CYCGRG 1 cut(s) 83
Bsp120I GGGCCC 1 cut(s) 1715
Bsp1286I GDGCHC 3 cut(s) 570, 1548, 1719
Bsp143I GATC 8 cut(s) 52, 309, 1214, 1218, 1304, 1594, 1612, 1688
Bsp19I CCATGG 1 cut(s) 48
BspANI GGCC 7 cut(s) 164, 258, 302, 559, 574, 1394, 1717
BspCNI CTCAG 5 cut(s) 397, 439, 582, 624, 1564
BspDI ATCGAT 2 cut(s) 1217, 1593
BspFNI CGCG 1 cut(s) 644
BspHI TCATGA 1 cut(s) 1243
BspPI GGATC 7 cut(s) 47, 60, 304, 1213, 1312, 1607, 1696
BspT107I GGYRCC 2 cut(s) 1097, 1599
BspTI CTTAAG 1 cut(s) 1661
BspTNI GGTCTC 2 cut(s) 787, 1660
BsrBI CCGCTC 3 cut(s) 180, 238, 315
BsrDI GCAATG 2 cut(s) 972, 992
BsrFI RCCGGY 2 cut(s) 469, 778
BsrI ACTGG 2 cut(s) 902, 1486
BssAI RCCGGY 2 cut(s) 469, 778
BssECI CCNNGG 7 cut(s) 48, 77, 252, 259, 1372, 1420, 1453
BssMI GATC 8 cut(s) 52, 309, 1214, 1218, 1304, 1594, 1612, 1688
BssNI GRCGYC 2 cut(s) 120, 806
BssT1I CCWWGG 4 cut(s) 48, 252, 1420, 1453
Bst2UI CCWGG 1 cut(s) 1373
Bst4CI ACNGT 4 cut(s) 436, 775, 841, 1465
BstACI GRCGYC 2 cut(s) 120, 806
BstAFI CTTAAG 1 cut(s) 1661
BstAPI GCANNNNNTGC 1 cut(s) 662
BstC8I GCNNGC 3 cut(s) 1296, 1317, 1518
BstDEI CTNAG 5 cut(s) 405, 447, 569, 632, 1551
BstDSI CCRYGG 1 cut(s) 48
BstEII GGTNACC 1 cut(s) 908
BstF5I GGATG 8 cut(s) 134, 331, 617, 1114, 1395, 1463, 1518, 1651
BstFNI CGCG 1 cut(s) 644
BstH2I RGCGCY 2 cut(s) 91, 750
BstHHI GCGC 4 cut(s) 90, 646, 656, 749
BstKTI GATC 8 cut(s) 55, 312, 1217, 1221, 1307, 1597, 1615, 1691
BstMAI GTCTC 3 cut(s) 764, 787, 1660
BstMBI GATC 8 cut(s) 52, 309, 1214, 1218, 1304, 1594, 1612, 1688
BstMCI CGRYCG 2 cut(s) 560, 1597
BstMWI GCNNNNNNNGC 7 cut(s) 500, 506, 509, 565, 574, 662, 1458
BstNI CCWGG 1 cut(s) 1373
BstNSI RCATGY 1 cut(s) 1298
BstPI GGTNACC 1 cut(s) 908
BstSCI CCNGG 3 cut(s) 207, 302, 1371
BstSLI GKGCMC 1 cut(s) 1719
BstUI CGCG 1 cut(s) 644
BstV1I GCAGC 6 cut(s) 427, 490, 493, 496, 707, 1621
BstV2I GAAGAC 1 cut(s) 1674
BstX2I RGATCY 3 cut(s) 52, 309, 1304
BstXI CCANNNNNNTGG 1 cut(s) 1427
BstYI RGATCY 3 cut(s) 52, 309, 1304
BstZI CGGCCG 1 cut(s) 557
Bsu15I ATCGAT 2 cut(s) 1217, 1593
Bsu36I CCTNAGG 1 cut(s) 1551
BsuRI GGCC 7 cut(s) 164, 258, 302, 559, 574, 1394, 1717
BsuTUI ATCGAT 2 cut(s) 1217, 1593
BtgI CCRYGG 1 cut(s) 48
BtrI CACGTC 1 cut(s) 1680
BtsCI GGATG 8 cut(s) 134, 331, 617, 1114, 1395, 1463, 1518, 1651
BtsIMutI CAGTG 3 cut(s) 837, 895, 1461
Cac8I GCNNGC 3 cut(s) 1296, 1317, 1518
CciI TCATGA 1 cut(s) 1243
CciNI GCGGCCGC 1 cut(s) 557
CfoI GCGC 4 cut(s) 90, 646, 656, 749
Cfr10I RCCGGY 2 cut(s) 469, 778
Cfr13I GGNCC 7 cut(s) 193, 256, 263, 301, 1393, 1715, 1716
ClaI ATCGAT 2 cut(s) 1217, 1593
CseI GACGC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 349
CviAII CATG 5 cut(s) 49, 1196, 1226, 1244, 1295
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 5 cut(s) 405, 447, 569, 632, 1551
DpnI GATC 8 cut(s) 54, 311, 1216, 1220, 1306, 1596, 1614, 1690
DpnII GATC 8 cut(s) 52, 309, 1214, 1218, 1304, 1594, 1612, 1688
DriI GACNNNNNGTC 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 557
EagI CGGCCG 1 cut(s) 557
Eam1105I GACNNNNNGTC 1 cut(s) 154
EclXI CGGCCG 1 cut(s) 557
Eco130I CCWWGG 4 cut(s) 48, 252, 1420, 1453
Eco24I GRGCYC 2 cut(s) 1548, 1719
Eco31I GGTCTC 2 cut(s) 787, 1660
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 2 cut(s) 193, 263
Eco52I CGGCCG 1 cut(s) 557
Eco57I CTGAAG 3 cut(s) 807, 1142, 1253
Eco81I CCTNAGG 1 cut(s) 1551
Eco88I CYCGRG 1 cut(s) 83
Eco91I GGTNACC 1 cut(s) 908
EcoO109I RGGNCCY 1 cut(s) 1715
EcoO65I GGTNACC 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 1371
EcoRV GATATC 1 cut(s) 282
EcoT14I CCWWGG 4 cut(s) 48, 252, 1420, 1453
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 1548, 1719
ErhI CCWWGG 4 cut(s) 48, 252, 1420, 1453
FaeI CATG 5 cut(s) 52, 1199, 1229, 1247, 1298
FalI AAGNNNNNCTT 2 cut(s) 1140, 1172
FaqI GGGAC 4 cut(s) 52, 638, 1280, 1390
FatI CATG 5 cut(s) 48, 1195, 1225, 1243, 1294
FauI CCCGC 5 cut(s) 91, 185, 473, 1479, 1704
FokI GGATG 8 cut(s) 121, 338, 624, 1121, 1402, 1470, 1525, 1638
FriOI GRGCYC 2 cut(s) 1548, 1719
FspBI CTAG 4 cut(s) 365, 458, 513, 828
GlaI GCGC 4 cut(s) 89, 645, 655, 748
GsaI CCCAGC 2 cut(s) 531, 1722
GsuI CTGGAG 1 cut(s) 1154
HaeII RGCGCY 2 cut(s) 91, 750
HaeIII GGCC 7 cut(s) 164, 258, 302, 559, 574, 1394, 1717
HapII CCGG 7 cut(s) 70, 196, 208, 304, 470, 596, 779
HgaI GACGC 1 cut(s) 128
HhaI GCGC 4 cut(s) 90, 646, 656, 749
Hin1I GRCGYC 2 cut(s) 120, 806
Hin1II CATG 5 cut(s) 52, 1199, 1229, 1247, 1298
Hin6I GCGC 4 cut(s) 88, 644, 654, 747
HinP1I GCGC 4 cut(s) 88, 644, 654, 747
HincII GTYRAC 3 cut(s) 453, 1408, 1479
HindII GTYRAC 3 cut(s) 453, 1408, 1479
HindIII AAGCTT 2 cut(s) 967, 1313
HinfI GANTC 8 cut(s) 149, 454, 464, 821, 1060, 1112, 1190, 1562
HpaI GTTAAC 2 cut(s) 1408, 1479
HpaII CCGG 7 cut(s) 70, 196, 208, 304, 470, 596, 779
HphI GGTGA 3 cut(s) 610, 770, 920
Hpy166II GTNNAC 4 cut(s) 453, 1261, 1408, 1479
Hpy188III TCNNGA 9 cut(s) 277, 342, 365, 1133, 1244, 1387, 1505, 1559, 1686
Hpy8I GTNNAC 4 cut(s) 453, 1261, 1408, 1479
Hpy99I CGWCG 9 cut(s) 122, 149, 160, 163, 272, 386, 611, 808, 811
HpyAV CCTTC 9 cut(s) 644, 719, 749, 782, 898, 923, 976, 1166, 1682
HpyCH4III ACNGT 4 cut(s) 436, 775, 841, 1465
HpyCH4IV ACGT 3 cut(s) 806, 1175, 1679
HpyF10VI GCNNNNNNNGC 7 cut(s) 500, 506, 509, 565, 574, 662, 1458
HpyF3I CTNAG 5 cut(s) 405, 447, 569, 632, 1551
HpySE526I ACGT 3 cut(s) 806, 1175, 1679
Hsp92I GRCGYC 2 cut(s) 120, 806
Hsp92II CATG 5 cut(s) 52, 1199, 1229, 1247, 1298
HspAI GCGC 4 cut(s) 88, 644, 654, 747
KspAI GTTAAC 2 cut(s) 1408, 1479
Kzo9I GATC 8 cut(s) 52, 309, 1214, 1218, 1304, 1594, 1612, 1688
LmnI GCTCC 3 cut(s) 235, 1135, 1449
Lsp1109I GCAGC 6 cut(s) 427, 490, 493, 496, 707, 1621
LweI GCATC 7 cut(s) 13, 487, 602, 1448, 1503, 1567, 1703
MaeI CTAG 4 cut(s) 365, 458, 513, 828
MaeII ACGT 3 cut(s) 806, 1175, 1679
MaeIII GTNAC 2 cut(s) 430, 908
MalI GATC 8 cut(s) 54, 311, 1216, 1220, 1306, 1596, 1614, 1690
MbiI CCGCTC 3 cut(s) 180, 238, 315
MboI GATC 8 cut(s) 52, 309, 1214, 1218, 1304, 1594, 1612, 1688
MflI RGATCY 3 cut(s) 52, 309, 1304
MhlI GDGCHC 3 cut(s) 570, 1548, 1719
MluCI AATT 2 cut(s) 386, 858
MlyI GAGTC 4 cut(s) 143, 448, 458, 1069
MmeI TCCRAC 3 cut(s) 336, 715, 1449
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 7 cut(s) 546, 1362, 1407, 1478, 1527, 1646, 1662
MslI CAYNNNNRTG 3 cut(s) 977, 1289, 1299
MspA1I CMGCKG 4 cut(s) 527, 1439, 1711, 1722
MspCI CTTAAG 1 cut(s) 1661
MspI CCGG 7 cut(s) 70, 196, 208, 304, 470, 596, 779
MspR9I CCNGG 3 cut(s) 209, 304, 1373
Mva1269I GAATGC 1 cut(s) 1382
MvaI CCWGG 1 cut(s) 1373
MvnI CGCG 1 cut(s) 644
MwoI GCNNNNNNNGC 7 cut(s) 500, 506, 509, 565, 574, 662, 1458
NciI CCSGG 2 cut(s) 209, 304
NcoI CCATGG 1 cut(s) 48
NdeII GATC 8 cut(s) 52, 309, 1214, 1218, 1304, 1594, 1612, 1688
NlaIII CATG 5 cut(s) 52, 1199, 1229, 1247, 1298
NmeAIII GCCGAG 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 908
NotI GCGGCCGC 1 cut(s) 557
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 1298
PaeI GCATGC 1 cut(s) 1298
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 1243
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 267
PctI GAATGC 1 cut(s) 1382
PfeI GAWTC 4 cut(s) 821, 1112, 1190, 1562
Ple19I CGATCG 1 cut(s) 1597
PleI GAGTC 4 cut(s) 143, 448, 458, 1068
PpsI GAGTC 4 cut(s) 143, 448, 458, 1068
PsiI TTATAA 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 1371
PspEI GGTNACC 1 cut(s) 908
PspFI CCCAGC 2 cut(s) 527, 1718
PspGI CCWGG 1 cut(s) 1371
PspOMI GGGCCC 1 cut(s) 1715
PspPI GGNCC 7 cut(s) 193, 256, 263, 301, 1393, 1715, 1716
PspXI VCTCGAGB 1 cut(s) 83
PsuI RGATCY 3 cut(s) 52, 309, 1304
PvuI CGATCG 1 cut(s) 1597
PvuII CAGCTG 3 cut(s) 527, 1439, 1722
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 3 cut(s) 977, 1289, 1299
SaqAI TTAA 7 cut(s) 546, 1362, 1407, 1478, 1527, 1646, 1662
Sau3AI GATC 8 cut(s) 52, 309, 1214, 1218, 1304, 1594, 1612, 1688
Sau96I GGNCC 7 cut(s) 193, 256, 263, 301, 1393, 1715, 1716
SchI GAGTC 4 cut(s) 143, 448, 458, 1069
ScrFI CCNGG 3 cut(s) 209, 304, 1373
SduI GDGCHC 3 cut(s) 570, 1548, 1719
SfaNI GCATC 7 cut(s) 13, 487, 602, 1448, 1503, 1567, 1703
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 2 cut(s) 193, 263
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 3 cut(s) 977, 1289, 1299
SmlI CTYRAG 2 cut(s) 83, 1661
SmoI CTYRAG 2 cut(s) 83, 1661
SphI GCATGC 1 cut(s) 1298
Sse9I AATT 2 cut(s) 386, 858
SspI AATATT 1 cut(s) 1091
SspMI CTAG 4 cut(s) 365, 458, 513, 828
StyD4I CCNGG 3 cut(s) 207, 302, 1371
StyI CCWWGG 4 cut(s) 48, 252, 1420, 1453
TaaI ACNGT 4 cut(s) 436, 775, 841, 1465
TaiI ACGT 3 cut(s) 809, 1178, 1682
TaqII GACCGA 1 cut(s) 1263
TasI AATT 2 cut(s) 386, 858
TauI GCSGC 6 cut(s) 125, 503, 559, 562, 577, 580
TfiI GAWTC 4 cut(s) 821, 1112, 1190, 1562
Tru1I TTAA 7 cut(s) 546, 1362, 1407, 1478, 1527, 1646, 1662
Tru9I TTAA 7 cut(s) 546, 1362, 1407, 1478, 1527, 1646, 1662
TscAI CASTG 3 cut(s) 844, 902, 1468
TseFI GTSAC 1 cut(s) 908
TseI GCWGC 6 cut(s) 440, 503, 506, 509, 695, 1634
Tsp45I GTSAC 1 cut(s) 908
TspDTI ATGAA 9 cut(s) 729, 786, 960, 961, 981, 1125, 1232, 1367, 1579
TspGWI ACGGA 1 cut(s) 624
TspRI CASTG 3 cut(s) 844, 902, 1468
Vha464I CTTAAG 1 cut(s) 1661
VpaK11BI GGWCC 2 cut(s) 193, 263
XapI RAATTY 1 cut(s) 858
XbaI TCTAGA 1 cut(s) 364
XceI RCATGY 1 cut(s) 1298
XcmI CCANNNNNNNNNTGG 1 cut(s) 1443
XhoI CTCGAG 1 cut(s) 83
XspI CTAG 4 cut(s) 365, 458, 513, 828
ZraI GACGTC 1 cut(s) 807
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.