Rw0G023410

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01079
OG0000243
Physical Location & Seq
Forward (+)
388059 .. 393024
4966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G023410.1

Sequence Viewer

Length: 1026 bp
ATGTCAGAATTATTTCGTTCATCAGCTCTTAAGCGGTTTCTTGAGGGGTTTCAGGGCACCGACAAAGATCTTGTCGAAGACATTGTGGAAAATGTGTACAGTAAACTACAATCTGTGGTGGTATCCCAGAGCCCTGACTCGACAAAAATTCAAGGCACATTGGCGGGGCCGAGCGGGTGTTGTTCTGGGAAGCCTAAGCTTACTGTCACTAATTGGAAAAAGGGTGATCTTCTCGGCAGCGGCTCTTTCGGGTCTGTGTATGAAGGAATTACTGATTGTGGACGCTTCATTGCTGTCAAGGAAGTTCCCTTGCTTGATCAAAGAAGAGTATCTCAACTTGAACAGGAGATTGCTCTTCTGAGCCAGTTTGAACATGAGAACATAGTTCAATATTATGGCACACAAAAGGATGAATCAAAGCTCTATATCTTGCTGGAACTTGTAACCGAAGGCTCCCTTCGAAAGCTATATCAAACATACCCTCTTACAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCATGACCAAAATGTGATTCACAGGGACATTAAATGCGCAAATATTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCGGACTTTGGATTGGCAAAGGCTATCAAAACGAACGACATGTTATCTTGCCAAGGAACTGCAAACTGGATGGCCCCTGAGGTTGTTAACCCTGAAAGTCCAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTAGGGAGGTTCCATACCCTAATCTTGAATGGATGGCGGCAGCATGTAAAATTATGATGGGGAAGCTCCCTCTGGTTCCTGATTCTCTTTCAAGAGAGGCACAAGATTTTATCCATCGATGCTTACAAGTTAAGCCAGATAATCGTCCCACTGCTGCTCAGCTTTTAGAACATCCGTTTGTAAATAAGCCCCTTCCTCCCACGTCTTCTGGATCAGTATCTCCTTACAATCACCACAGGCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

341

Amino Acids

37.87

Weight (kDa)

6.32

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 73 - 320 2.5e-70 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 75 - 316 4.2e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 56
AccB7I CCANNNNNTGG 1 cut(s) 725
AccBSI CCGCTC 1 cut(s) 174
AccI GTMKAC 1 cut(s) 507
AciI CCGC 6 cut(s) 34, 164, 174, 240, 620, 815
AclWI GGATC 2 cut(s) 613, 997
AcsI RAATTY 1 cut(s) 147
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 2 cut(s) 98, 760
AfiI CCNNNNNNNGG 1 cut(s) 725
AflII CTTAAG 1 cut(s) 29
AflIII ACRYGT 1 cut(s) 657
AgsI TTSAA 6 cut(s) 152, 341, 371, 389, 806, 870
AjiI CACGTC 1 cut(s) 981
AjnI CCWGG 1 cut(s) 718
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AluBI AGCT 8 cut(s) 26, 199, 421, 466, 616, 737, 844, 940
AluI AGCT 8 cut(s) 26, 199, 421, 466, 616, 737, 844, 940
Alw26I GTCTC 1 cut(s) 505
AlwI GGATC 2 cut(s) 613, 997
AoxI GGCC 3 cut(s) 167, 690, 727
ApeKI GCWGC 3 cut(s) 237, 818, 932
ApoI RAATTY 1 cut(s) 147
ArsI GACNNNNNNTTYG 2 cut(s) 57, 89
Asp700I GAANNNNTTC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 581
AspS9I GGNCC 2 cut(s) 167, 691
AsuHPI GGTGA 2 cut(s) 236, 1001
AsuII TTCGAA 1 cut(s) 460
AxyI CCTNAGG 1 cut(s) 696
BaeGI GKGCMC 1 cut(s) 59
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 134
BbsI GAAGAC 2 cut(s) 84, 975
BbvI GCAGC 3 cut(s) 249, 830, 919
BccI CCATC 4 cut(s) 682, 805, 829, 900
BciT130I CCWGG 1 cut(s) 720
BciVI GTATCC 1 cut(s) 133
BclI TGATCA 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 2 cut(s) 513, 780
BfrI CTTAAG 1 cut(s) 29
BfuI GTATCC 1 cut(s) 133
BglII AGATCT 2 cut(s) 67, 519
BisI GCNGC 5 cut(s) 238, 241, 816, 819, 933
BlpI GCTNAGC 1 cut(s) 936
BlsI GCNGC 5 cut(s) 239, 242, 817, 820, 934
Bme1390I CCNGG 1 cut(s) 720
BmgBI CACGTC 1 cut(s) 981
BmgT120I GGNCC 2 cut(s) 167, 691
BmiI GGNNCC 6 cut(s) 58, 168, 454, 693, 789, 855
BmrFI CCNGG 1 cut(s) 720
BmsI GCATC 1 cut(s) 887
BpiI GAAGAC 2 cut(s) 84, 975
Bpu10I CCTNAGC 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 460
BpuEI CTTGAG 1 cut(s) 62
Bsa29I ATCGAT 1 cut(s) 895
BsaBI GATNNNNATC 1 cut(s) 994
BsaJI CCNNGG 1 cut(s) 670
Bsc4I CCNNNNNNNGG 1 cut(s) 725
Bse1I ACTGG 2 cut(s) 364, 689
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 288
Bse8I GATNNNNATC 1 cut(s) 994
BseBI CCWGG 1 cut(s) 720
BseCI ATCGAT 1 cut(s) 895
BseDI CCNNGG 1 cut(s) 670
BseGI GGATG 5 cut(s) 415, 693, 761, 816, 949
BseJI GATNNNNATC 1 cut(s) 994
BseLI CCNNNNNNNGG 1 cut(s) 725
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 3 cut(s) 350, 687, 950
BseNI ACTGG 2 cut(s) 364, 689
BseSI GKGCMC 1 cut(s) 59
BseXI GCAGC 3 cut(s) 249, 830, 919
BshFI GGCC 3 cut(s) 169, 692, 729
BshNI GGYRCC 1 cut(s) 56
BshVI ATCGAT 1 cut(s) 895
BslFI GGGAC 2 cut(s) 581, 909
BslI CCNNNNNNNGG 1 cut(s) 725
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 581, 909
BsnI GGCC 3 cut(s) 169, 692, 729
Bsp119I TTCGAA 1 cut(s) 460
Bsp1286I GDGCHC 2 cut(s) 59, 134
Bsp1407I TGTACA 1 cut(s) 96
Bsp143I GATC 6 cut(s) 67, 226, 316, 519, 605, 989
Bsp1720I GCTNAGC 1 cut(s) 936
BspACI CCGC 6 cut(s) 34, 164, 174, 240, 620, 815
BspANI GGCC 3 cut(s) 169, 692, 729
BspCNI CTCAG 3 cut(s) 351, 688, 949
BspDI ATCGAT 1 cut(s) 895
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 6 cut(s) 58, 168, 454, 693, 789, 855
BspPI GGATC 2 cut(s) 613, 997
BspQI GCTCTTC 1 cut(s) 360
BspT104I TTCGAA 1 cut(s) 460
BspT107I GGYRCC 1 cut(s) 56
BspTI CTTAAG 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 288
BsrGI TGTACA 1 cut(s) 96
BsrI ACTGG 2 cut(s) 364, 689
BssECI CCNNGG 1 cut(s) 670
BssMI GATC 6 cut(s) 67, 226, 316, 519, 605, 989
BssNAI GTATAC 1 cut(s) 508
BssT1I CCWWGG 1 cut(s) 670
Bst1107I GTATAC 1 cut(s) 508
Bst2UI CCWGG 1 cut(s) 720
Bst4CI ACNGT 3 cut(s) 101, 205, 763
Bst6I CTCTTC 2 cut(s) 319, 360
BstAFI CTTAAG 1 cut(s) 29
BstAUI TGTACA 1 cut(s) 96
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 597, 618
BstDEI CTNAG 4 cut(s) 195, 359, 696, 936
BstF5I GGATG 5 cut(s) 415, 693, 761, 816, 949
BstHHI GCGC 1 cut(s) 581
BstKTI GATC 6 cut(s) 70, 229, 319, 522, 608, 992
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 6 cut(s) 67, 226, 316, 519, 605, 989
BstNI CCWGG 1 cut(s) 720
BstNSI RCATGY 4 cut(s) 500, 599, 661, 825
BstSCI CCNGG 1 cut(s) 718
BstSLI GKGCMC 1 cut(s) 59
BstV1I GCAGC 3 cut(s) 249, 830, 919
BstV2I GAAGAC 2 cut(s) 84, 975
BstX2I RGATCY 3 cut(s) 67, 519, 605
BstYI RGATCY 3 cut(s) 67, 519, 605
BstZ17I GTATAC 1 cut(s) 508
Bsu15I ATCGAT 1 cut(s) 895
Bsu36I CCTNAGG 1 cut(s) 696
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 3 cut(s) 169, 692, 729
BsuTUI ATCGAT 1 cut(s) 895
BtrI CACGTC 1 cut(s) 981
BtsCI GGATG 5 cut(s) 415, 693, 761, 816, 949
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 1 cut(s) 927
Cac8I GCNNGC 2 cut(s) 597, 618
CciI TCATGA 1 cut(s) 544
CfoI GCGC 1 cut(s) 581
Cfr13I GGNCC 2 cut(s) 167, 691
ClaI ATCGAT 1 cut(s) 895
CseI GACGC 1 cut(s) 291
Csp6I GTAC 2 cut(s) 97, 759
CviAII CATG 6 cut(s) 374, 497, 545, 596, 658, 822
CviQI GTAC 2 cut(s) 97, 759
DdeI CTNAG 4 cut(s) 195, 359, 696, 936
DpnI GATC 6 cut(s) 69, 228, 318, 521, 607, 991
DpnII GATC 6 cut(s) 67, 226, 316, 519, 605, 989
Eam1104I CTCTTC 2 cut(s) 319, 360
EarI CTCTTC 2 cut(s) 319, 360
Eco130I CCWWGG 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 134
Eco57I CTGAAG 1 cut(s) 554
Eco81I CCTNAGG 1 cut(s) 696
EcoRII CCWGG 1 cut(s) 718
EcoT14I CCWWGG 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 670
FaeI CATG 6 cut(s) 377, 500, 548, 599, 661, 825
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FaqI GGGAC 2 cut(s) 581, 909
FatI CATG 6 cut(s) 373, 496, 544, 595, 657, 821
FauI CCCGC 2 cut(s) 157, 167
FbaI TGATCA 1 cut(s) 316
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 5 cut(s) 238, 241, 816, 819, 933
FokI GGATG 5 cut(s) 422, 700, 768, 823, 936
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 5 cut(s) 238, 241, 816, 819, 933
FspBI CTAG 2 cut(s) 513, 780
FspI TGCGCA 1 cut(s) 580
GlaI GCGC 1 cut(s) 580
GluI GCNGC 5 cut(s) 238, 241, 816, 819, 933
HaeIII GGCC 3 cut(s) 169, 692, 729
HgaI GACGC 1 cut(s) 291
HhaI GCGC 1 cut(s) 581
Hin1II CATG 6 cut(s) 377, 500, 548, 599, 661, 825
Hin6I GCGC 1 cut(s) 579
HinP1I GCGC 1 cut(s) 579
HincII GTYRAC 2 cut(s) 706, 777
HindII GTYRAC 2 cut(s) 706, 777
HindIII AAGCTT 2 cut(s) 197, 614
HinfI GANTC 6 cut(s) 137, 413, 491, 559, 748, 860
HpaI GTTAAC 2 cut(s) 706, 777
HphI GGTGA 2 cut(s) 236, 1001
Hpy166II GTNNAC 6 cut(s) 97, 104, 281, 508, 706, 777
Hpy188I TCNGA 3 cut(s) 7, 360, 534
Hpy188III TCNNGA 6 cut(s) 41, 545, 803, 857, 870, 987
Hpy8I GTNNAC 6 cut(s) 97, 104, 281, 508, 706, 777
HpyAV CCTTC 5 cut(s) 257, 443, 467, 740, 980
HpyCH4III ACNGT 3 cut(s) 101, 205, 763
HpyCH4IV ACGT 1 cut(s) 980
HpyCH4V TGCA 3 cut(s) 526, 595, 680
HpyF3I CTNAG 4 cut(s) 195, 359, 696, 936
HpySE526I ACGT 1 cut(s) 980
Hsp92II CATG 6 cut(s) 377, 500, 548, 599, 661, 825
HspAI GCGC 1 cut(s) 579
Ksp22I TGATCA 1 cut(s) 316
KspAI GTTAAC 2 cut(s) 706, 777
Kzo9I GATC 6 cut(s) 67, 226, 316, 519, 605, 989
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 2 cut(s) 458, 849
Lsp1109I GCAGC 3 cut(s) 249, 830, 919
LweI GCATC 1 cut(s) 887
MaeI CTAG 2 cut(s) 513, 780
MaeII ACGT 1 cut(s) 980
MaeIII GTNAC 2 cut(s) 205, 442
MalI GATC 6 cut(s) 69, 228, 318, 521, 607, 991
MbiI CCGCTC 1 cut(s) 174
MboI GATC 6 cut(s) 67, 226, 316, 519, 605, 989
MboII GAAGA 6 cut(s) 89, 221, 336, 347, 533, 975
MflI RGATCY 3 cut(s) 67, 519, 605
MhlI GDGCHC 2 cut(s) 59, 134
MluCI AATT 5 cut(s) 8, 147, 211, 267, 828
MlyI GAGTC 2 cut(s) 131, 757
MmeI TCCRAC 1 cut(s) 747
MnlI CCTC 7 cut(s) 37, 492, 691, 778, 858, 868, 984
MroXI GAANNNNTTC 1 cut(s) 12
MseI TTAA 5 cut(s) 30, 573, 705, 776, 909
MslI CAYNNNNRTG 1 cut(s) 600
MspA1I CMGCKG 2 cut(s) 240, 737
MspCI CTTAAG 1 cut(s) 29
MspR9I CCNGG 1 cut(s) 720
MvaI CCWGG 1 cut(s) 720
NdeII GATC 6 cut(s) 67, 226, 316, 519, 605, 989
NlaIII CATG 6 cut(s) 377, 500, 548, 599, 661, 825
NlaIV GGNNCC 6 cut(s) 58, 168, 454, 693, 789, 855
NmeAIII GCCGAG 2 cut(s) 195, 213
NmuCI GTSAC 1 cut(s) 205
NsbI TGCGCA 1 cut(s) 580
NspI RCATGY 4 cut(s) 500, 599, 661, 825
NspV TTCGAA 1 cut(s) 460
PaeI GCATGC 1 cut(s) 599
PagI TCATGA 1 cut(s) 544
PciI ACATGT 1 cut(s) 657
PciSI GCTCTTC 1 cut(s) 360
PdmI GAANNNNTTC 1 cut(s) 12
PfeI GAWTC 4 cut(s) 413, 491, 559, 860
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 5 cut(s) 239, 242, 817, 820, 934
PleI GAGTC 2 cut(s) 131, 756
PpsI GAGTC 2 cut(s) 131, 756
PscI ACATGT 1 cut(s) 657
Psp6I CCWGG 1 cut(s) 718
PspGI CCWGG 1 cut(s) 718
PspN4I GGNNCC 6 cut(s) 58, 168, 454, 693, 789, 855
PspPI GGNCC 2 cut(s) 167, 691
PsuI RGATCY 3 cut(s) 67, 519, 605
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 2 cut(s) 98, 760
RsaNI GTAC 2 cut(s) 97, 759
RseI CAYNNNNRTG 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 5 cut(s) 30, 573, 705, 776, 909
SatI GCNGC 5 cut(s) 238, 241, 816, 819, 933
Sau3AI GATC 6 cut(s) 67, 226, 316, 519, 605, 989
Sau96I GGNCC 2 cut(s) 167, 691
SchI GAGTC 2 cut(s) 131, 757
ScrFI CCNGG 1 cut(s) 720
SduI GDGCHC 2 cut(s) 59, 134
SfaNI GCATC 1 cut(s) 887
SfuI TTCGAA 1 cut(s) 460
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 2 cut(s) 29, 41
SmoI CTYRAG 2 cut(s) 29, 41
SphI GCATGC 1 cut(s) 599
Sse9I AATT 5 cut(s) 8, 147, 211, 267, 828
SsiI CCGC 6 cut(s) 34, 164, 174, 240, 620, 815
SspI AATATT 2 cut(s) 392, 586
SspMI CTAG 2 cut(s) 513, 780
StyD4I CCNGG 1 cut(s) 718
StyI CCWWGG 1 cut(s) 670
TaaI ACNGT 3 cut(s) 101, 205, 763
TaiI ACGT 1 cut(s) 983
TaqI TCGA 4 cut(s) 75, 140, 460, 895
TasI AATT 5 cut(s) 8, 147, 211, 267, 828
TatI WGTACW 2 cut(s) 96, 758
TauI GCSGC 2 cut(s) 243, 818
TfiI GAWTC 4 cut(s) 413, 491, 559, 860
Tru1I TTAA 5 cut(s) 30, 573, 705, 776, 909
Tru9I TTAA 5 cut(s) 30, 573, 705, 776, 909
TscAI CASTG 1 cut(s) 934
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 3 cut(s) 237, 818, 932
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 5 cut(s) 9, 276, 277, 426, 533
TspGWI ACGGA 1 cut(s) 942
TspRI CASTG 1 cut(s) 934
Van91I CCANNNNNTGG 1 cut(s) 725
Vha464I CTTAAG 1 cut(s) 29
XapI RAATTY 1 cut(s) 147
XceI RCATGY 4 cut(s) 500, 599, 661, 825
XcmI CCANNNNNNNNNTGG 1 cut(s) 741
XmiI GTMKAC 1 cut(s) 507
XmnI GAANNNNTTC 1 cut(s) 12
XspI CTAG 2 cut(s) 513, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.