Rw7G041870

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000243
Physical Location & Seq
Forward (+)
65461299 .. 65466558
5260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041870.1

Sequence Viewer

Length: 1749 bp
ATGCATCACTTACCGCGTCTTTTCTCCAGCAACAAGGATCGAGCTAAATCCATGGATCAAAAGAAGGCCCGGCGGTTGCAGCGCCGCAATGCGGTCAAGCACATTGACTACGACGCCTCTTCGTCCTCCTCCTCCCGCGACAACTCCACCCACGCGCAGCAGCTCCACACGCGCTCGCTCGACCTGTCGGACCGGACCAGCTTCCGGGTCGAAGGAAATGACGGCGAGTTCGAAAGGATTTGCGCCGAGCTGGGCTTCTCCGGCCCAGACGACTTCGCAATACCCGAGGCGGCCTGGGAGTCCCGGAAGATCCGGTCCACGAACTCGGATGTTCTCCCCCTGTCAAAACTGTACGAGATGGATAGTCCGACACCCGACCCGAAGGACGAATCGGAGCGTGAGGATGATGCCGTGGCGGAATTGAGCAATAGAGTTAGGGATAGCGTTACTGTTACGGTGGCCGAGTCGACTCGGACCGAGTCCGAGTCGGCGGGGCCGAGCGGGTATTGTACTGCGAGTAGTAATAGTTGTAGTGTTAGTGTCGGAATTAAGGGAGCGCGGCCGCCGGTGCTGAAGCCACCGCCGTCGATGGTTAGGGTTCAGGTGATTGACAATGGATGCTCGACTTGGGACATTTTTAGGGACTTGGCTCCAGAAGAAGAGAGGGATATGGTGCGCAGCAGGTTTGTTCCCTCTTCTTCTTCTGATGAAGAAGATGAAGAACAAGAACTAGAAGAAGAACTTGAAGAAGGAGAGGATGTTGTGGCGGATGAGATTAGAGAAACCGTGGCAACCTCCGGCGGGTGTTCGTTTACTACTTCCAATGACGACGACTCTTCCAGCACTACCACAGAGCCTTCAAATATTTCTCCAAATGGAAGGTTTTCGCCGAATGGGAAGCCTAAGCTTATTGTCACTAGTTGGGAGAAGGGTGATCTTCTCGGCAGCGGCTCTTTCGGGTCTGTGTATGAAGGAATTTCTGATGGCGGATGCTTCATTGCTGTTAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGAAGGCAAAGAGTTACTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGAATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAGACGTACCATCTTACAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCGAAGAGTGATTCACAGGGACGTTAAATGTGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCAGACTTTGGATTGGCAAAGACTATCAAAATGAACGACATAAAATCTTGCCAAGGAACTGCATACTGGATGGCACCTGAGGTTGTTAATCGTAAGAGTCAAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACTCTAATCTCGAATGGATGCAGGCATTATGGAAAATTGGAAAGGGGGAGCCCCCTCTGGTTCCTGATTCTCTTTCAAAAGAGGCACAAGATTTCATCCGTCTATGCTTACAAGTTAAGCCAGATAATCGTCCCACTGCTGCTCAGCTTTTAAAACATCCATTTGTAAATAAGCCCCTTCTTCCCACGTCTTCTGGATCAGTATCTCCTCACAATCACCACAGGCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

582

Amino Acids

64.61

Weight (kDa)

5.31

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 309 - 561 5.4e-69 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 311 - 557 7.4e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 677
Acc36I ACCTGC 1 cut(s) 672
AccB1I GGYRCC 1 cut(s) 1414
AccBSI CCGCTC 1 cut(s) 501
AccI GTMKAC 2 cut(s) 467, 1230
AccII CGCG 5 cut(s) 16, 138, 155, 172, 559
AclWI GGATC 5 cut(s) 45, 63, 304, 1336, 1720
AcoI YGGCCR 2 cut(s) 459, 560
AcsI RAATTY 1 cut(s) 975
AcuI CTGAAG 2 cut(s) 593, 1277
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 4 cut(s) 353, 511, 1202, 1483
AfiI CCNNNNNNNGG 3 cut(s) 91, 204, 801
AgsI TTSAA 6 cut(s) 746, 861, 1064, 1094, 1184, 1593
AhlI ACTAGT 1 cut(s) 917
AjiI CACGTC 1 cut(s) 1704
AjnI CCWGG 2 cut(s) 293, 1035
Alw26I GTCTC 1 cut(s) 1228
AlwI GGATC 5 cut(s) 45, 63, 304, 1336, 1720
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 7 cut(s) 66, 262, 291, 459, 494, 560, 1450
ApeKI GCWGC 6 cut(s) 79, 157, 160, 678, 945, 1655
ApoI RAATTY 1 cut(s) 975
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
Asp700I GAANNNNTTC 1 cut(s) 883
AspLEI GCGC 6 cut(s) 84, 157, 174, 245, 559, 678
AspS9I GGNCC 7 cut(s) 67, 190, 195, 263, 315, 474, 494
AsuC2I CCSGG 3 cut(s) 70, 206, 304
AsuHPI GGTGA 3 cut(s) 616, 944, 1724
AsuII TTCGAA 1 cut(s) 231
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 4 cut(s) 190, 195, 315, 474
AxyI CCTNAGG 1 cut(s) 1419
BanI GGYRCC 1 cut(s) 1414
BanII GRGCYC 1 cut(s) 1569
BbsI GAAGAC 1 cut(s) 1698
BbvI GCAGC 6 cut(s) 91, 169, 172, 690, 957, 1642
BccI CCATC 6 cut(s) 352, 583, 977, 1212, 1405, 1501
BceAI ACGGC 3 cut(s) 238, 395, 568
BciT130I CCWGG 2 cut(s) 295, 1037
BclI TGATCA 1 cut(s) 1024
BcnI CCSGG 3 cut(s) 70, 206, 304
BcoDI GTCTC 1 cut(s) 1228
BcuI ACTAGT 1 cut(s) 917
BfaI CTAG 3 cut(s) 731, 918, 1236
BfoI RGCGCY 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 672
BglII AGATCT 1 cut(s) 1242
BlpI GCTNAGC 1 cut(s) 1659
Bme1390I CCNGG 5 cut(s) 70, 206, 295, 304, 1037
Bme18I GGWCC 4 cut(s) 190, 195, 315, 474
BmeT110I CYCGRG 1 cut(s) 284
BmgBI CACGTC 1 cut(s) 1704
BmgT120I GGNCC 7 cut(s) 67, 190, 195, 263, 315, 474, 494
BmiI GGNNCC 7 cut(s) 495, 651, 1177, 1416, 1512, 1566, 1578
BmrFI CCNGG 5 cut(s) 70, 206, 295, 304, 1037
BmrI ACTGGG 1 cut(s) 1512
BmsI GCATC 5 cut(s) 13, 397, 608, 980, 1524
BmuI ACTGGG 1 cut(s) 1512
BpiI GAAGAC 1 cut(s) 1698
BpmI CTGGAG 2 cut(s) 10, 636
Bpu10I CCTNAGC 1 cut(s) 903
Bpu1102I GCTNAGC 1 cut(s) 1659
Bpu14I TTCGAA 1 cut(s) 231
BpuMI CCSGG 3 cut(s) 70, 206, 304
BsaBI GATNNNNATC 1 cut(s) 1717
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 7 cut(s) 51, 285, 294, 411, 786, 1036, 1393
BsaWI WCCGGW 2 cut(s) 192, 312
BsaXI ACNNNNNCTCC 2 cut(s) 744, 774
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 204, 801
Bse118I RCCGGY 1 cut(s) 565
Bse1I ACTGG 2 cut(s) 1412, 1507
Bse21I CCTNAGG 1 cut(s) 1419
Bse3DI GCAATG 2 cut(s) 94, 996
Bse8I GATNNNNATC 1 cut(s) 1717
BseBI CCWGG 2 cut(s) 295, 1037
BseDI CCNNGG 7 cut(s) 51, 285, 294, 411, 786, 1036, 1393
BseJI GATNNNNATC 1 cut(s) 1717
BseLI CCNNNNNNNGG 3 cut(s) 91, 204, 801
BseMI GCAATG 2 cut(s) 94, 996
BseMII CTCAG 3 cut(s) 1073, 1410, 1673
BseNI ACTGG 2 cut(s) 1412, 1507
BseRI GAGGAG 3 cut(s) 118, 121, 1713
BseX3I CGGCCG 1 cut(s) 560
BseXI GCAGC 6 cut(s) 91, 169, 172, 690, 957, 1642
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 5 cut(s) 16, 138, 155, 172, 559
Bsh1285I CGRYCG 2 cut(s) 563, 1273
BshFI GGCC 7 cut(s) 68, 264, 293, 461, 496, 562, 1452
BshNI GGYRCC 1 cut(s) 1414
BsiEI CGRYCG 2 cut(s) 563, 1273
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 8 cut(s) 70, 193, 205, 261, 304, 313, 566, 798
BslFI GGGAC 5 cut(s) 286, 644, 656, 1304, 1632
BslI CCNNNNNNNGG 3 cut(s) 91, 204, 801
BsmAI GTCTC 1 cut(s) 1228
BsmFI GGGAC 5 cut(s) 286, 644, 656, 1304, 1632
BsnI GGCC 7 cut(s) 68, 264, 293, 461, 496, 562, 1452
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 1569
Bsp143I GATC 8 cut(s) 37, 55, 309, 934, 1024, 1242, 1328, 1712
Bsp1720I GCTNAGC 1 cut(s) 1659
Bsp19I CCATGG 1 cut(s) 51
BspANI GGCC 7 cut(s) 68, 264, 293, 461, 496, 562, 1452
BspCNI CTCAG 3 cut(s) 1074, 1411, 1672
BspFNI CGCG 5 cut(s) 16, 138, 155, 172, 559
BspLI GGNNCC 7 cut(s) 495, 651, 1177, 1416, 1512, 1566, 1578
BspMI ACCTGC 1 cut(s) 672
BspPI GGATC 5 cut(s) 45, 63, 304, 1336, 1720
BspQI GCTCTTC 1 cut(s) 1083
BspT104I TTCGAA 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 1414
BsrBI CCGCTC 1 cut(s) 501
BsrDI GCAATG 2 cut(s) 94, 996
BsrFI RCCGGY 1 cut(s) 565
BsrI ACTGG 2 cut(s) 1412, 1507
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 7 cut(s) 51, 285, 294, 411, 786, 1036, 1393
BssMI GATC 8 cut(s) 37, 55, 309, 934, 1024, 1242, 1328, 1712
BssNAI GTATAC 1 cut(s) 1231
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 51, 1393
Bst1107I GTATAC 1 cut(s) 1231
Bst2UI CCWGG 2 cut(s) 295, 1037
Bst4CI ACNGT 5 cut(s) 351, 451, 457, 787, 1486
Bst6I CTCTTC 6 cut(s) 124, 654, 700, 841, 1083, 1270
BstACI GRCGYC 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 4 cut(s) 176, 1320, 1341, 1539
BstDEI CTNAG 4 cut(s) 903, 1082, 1419, 1659
BstDSI CCRYGG 3 cut(s) 51, 411, 786
BstFNI CGCG 5 cut(s) 16, 138, 155, 172, 559
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 6 cut(s) 84, 157, 174, 245, 559, 678
BstKTI GATC 8 cut(s) 40, 58, 312, 937, 1027, 1245, 1331, 1715
BstMAI GTCTC 1 cut(s) 1228
BstMBI GATC 8 cut(s) 37, 55, 309, 934, 1024, 1242, 1328, 1712
BstMCI CGRYCG 2 cut(s) 563, 1273
BstMWI GCNNNNNNNGC 4 cut(s) 79, 169, 261, 568
BstNI CCWGG 2 cut(s) 295, 1037
BstNSI RCATGY 2 cut(s) 1223, 1322
BstSCI CCNGG 5 cut(s) 68, 204, 293, 302, 1035
BstUI CGCG 5 cut(s) 16, 138, 155, 172, 559
BstV1I GCAGC 6 cut(s) 91, 169, 172, 690, 957, 1642
BstV2I GAAGAC 1 cut(s) 1698
BstX2I RGATCY 3 cut(s) 309, 1242, 1328
BstYI RGATCY 3 cut(s) 309, 1242, 1328
BstZ17I GTATAC 1 cut(s) 1231
BstZI CGGCCG 1 cut(s) 560
Bsu36I CCTNAGG 1 cut(s) 1419
BsuRI GGCC 7 cut(s) 68, 264, 293, 461, 496, 562, 1452
BtgI CCRYGG 3 cut(s) 51, 411, 786
BtrI CACGTC 1 cut(s) 1704
BtsI GCAGTG 1 cut(s) 1650
BtsIMutI CAGTG 1 cut(s) 1650
BveI ACCTGC 1 cut(s) 672
Cac8I GCNNGC 4 cut(s) 176, 1320, 1341, 1539
CciNI GCGGCCGC 1 cut(s) 560
CfoI GCGC 6 cut(s) 84, 157, 174, 245, 559, 678
Cfr10I RCCGGY 1 cut(s) 565
Cfr13I GGNCC 7 cut(s) 67, 190, 195, 263, 315, 474, 494
CpoI CGGWCCG 2 cut(s) 190, 474
CseI GACGC 2 cut(s) 5, 122
Csp6I GTAC 4 cut(s) 352, 510, 1201, 1482
CspI CGGWCCG 2 cut(s) 190, 474
CviAII CATG 4 cut(s) 52, 1097, 1220, 1319
CviQI GTAC 4 cut(s) 352, 510, 1201, 1482
DdeI CTNAG 4 cut(s) 903, 1082, 1419, 1659
DpnI GATC 8 cut(s) 39, 57, 311, 936, 1026, 1244, 1330, 1714
DpnII GATC 8 cut(s) 37, 55, 309, 934, 1024, 1242, 1328, 1712
DraI TTTAAA 1 cut(s) 1668
EaeI YGGCCR 2 cut(s) 459, 560
EagI CGGCCG 1 cut(s) 560
Eam1104I CTCTTC 6 cut(s) 124, 654, 700, 841, 1083, 1270
EarI CTCTTC 6 cut(s) 124, 654, 700, 841, 1083, 1270
EciI GGCGGA 3 cut(s) 431, 782, 1002
EclXI CGGCCG 1 cut(s) 560
Eco130I CCWWGG 2 cut(s) 51, 1393
Eco24I GRGCYC 1 cut(s) 1569
Eco47I GGWCC 4 cut(s) 190, 195, 315, 474
Eco52I CGGCCG 1 cut(s) 560
Eco57I CTGAAG 2 cut(s) 593, 1277
Eco81I CCTNAGG 1 cut(s) 1419
Eco88I CYCGRG 1 cut(s) 284
EcoRII CCWGG 2 cut(s) 293, 1035
EcoT14I CCWWGG 2 cut(s) 51, 1393
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1569
ErhI CCWWGG 2 cut(s) 51, 1393
FaeI CATG 4 cut(s) 55, 1100, 1223, 1322
FalI AAGNNNNNCTT 4 cut(s) 726, 758, 1164, 1196
FaqI GGGAC 5 cut(s) 286, 644, 656, 1304, 1632
FatI CATG 4 cut(s) 51, 1096, 1219, 1318
FauI CCCGC 4 cut(s) 143, 484, 494, 794
FbaI TGATCA 1 cut(s) 1024
FblI GTMKAC 2 cut(s) 467, 1230
FriOI GRGCYC 1 cut(s) 1569
FspBI CTAG 3 cut(s) 731, 918, 1236
FspI TGCGCA 1 cut(s) 677
GlaI GCGC 6 cut(s) 83, 156, 173, 244, 558, 677
GsaI CCCAGC 1 cut(s) 254
GsuI CTGGAG 2 cut(s) 10, 636
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 7 cut(s) 68, 264, 293, 461, 496, 562, 1452
HapII CCGG 8 cut(s) 70, 193, 205, 261, 304, 313, 566, 798
HgaI GACGC 2 cut(s) 5, 122
HhaI GCGC 6 cut(s) 84, 157, 174, 245, 559, 678
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 4 cut(s) 55, 1100, 1223, 1322
Hin6I GCGC 6 cut(s) 82, 155, 172, 243, 557, 676
HinP1I GCGC 6 cut(s) 82, 155, 172, 243, 557, 676
HincII GTYRAC 2 cut(s) 468, 1500
HindII GTYRAC 2 cut(s) 468, 1500
HindIII AAGCTT 2 cut(s) 905, 1337
HpaI GTTAAC 1 cut(s) 1500
HpaII CCGG 8 cut(s) 70, 193, 205, 261, 304, 313, 566, 798
HphI GGTGA 3 cut(s) 616, 944, 1724
Hpy166II GTNNAC 5 cut(s) 318, 468, 813, 1231, 1500
Hpy188III TCNNGA 6 cut(s) 653, 1157, 1268, 1526, 1580, 1710
Hpy8I GTNNAC 5 cut(s) 318, 468, 813, 1231, 1500
Hpy99I CGWCG 3 cut(s) 116, 589, 833
HpyCH4III ACNGT 5 cut(s) 351, 451, 457, 787, 1486
HpyCH4IV ACGT 3 cut(s) 1199, 1293, 1703
HpyCH4V TGCA 8 cut(s) 4, 79, 1249, 1304, 1318, 1343, 1403, 1537
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 169, 261, 568
HpyF3I CTNAG 4 cut(s) 903, 1082, 1419, 1659
HpySE526I ACGT 3 cut(s) 1199, 1293, 1703
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 4 cut(s) 55, 1100, 1223, 1322
HspAI GCGC 6 cut(s) 82, 155, 172, 243, 557, 676
Ksp22I TGATCA 1 cut(s) 1024
KspAI GTTAAC 1 cut(s) 1500
Kzo9I GATC 8 cut(s) 37, 55, 309, 934, 1024, 1242, 1328, 1712
LguI GCTCTTC 1 cut(s) 1083
LmnI GCTCC 6 cut(s) 168, 394, 554, 655, 1181, 1564
Lsp1109I GCAGC 6 cut(s) 91, 169, 172, 690, 957, 1642
LweI GCATC 5 cut(s) 13, 397, 608, 980, 1524
MaeI CTAG 3 cut(s) 731, 918, 1236
MaeII ACGT 3 cut(s) 1199, 1293, 1703
MaeIII GTNAC 5 cut(s) 445, 451, 913, 1051, 1165
MalI GATC 8 cut(s) 39, 57, 311, 936, 1026, 1244, 1330, 1714
MbiI CCGCTC 1 cut(s) 501
MboI GATC 8 cut(s) 37, 55, 309, 934, 1024, 1242, 1328, 1712
MflI RGATCY 3 cut(s) 309, 1242, 1328
MhlI GDGCHC 1 cut(s) 1569
MluCI AATT 4 cut(s) 419, 546, 975, 1551
MlyI GAGTC 9 cut(s) 308, 463, 473, 488, 494, 827, 1093, 1447, 1480
MmeI TCCRAC 4 cut(s) 168, 392, 523, 1470
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 883
MseI TTAA 7 cut(s) 549, 1005, 1296, 1428, 1499, 1632, 1667
MslI CAYNNNNRTG 1 cut(s) 1323
MspA1I CMGCKG 2 cut(s) 948, 1460
MspI CCGG 8 cut(s) 70, 193, 205, 261, 304, 313, 566, 798
MspR9I CCNGG 5 cut(s) 70, 206, 295, 304, 1037
MvaI CCWGG 2 cut(s) 295, 1037
MvnI CGCG 5 cut(s) 16, 138, 155, 172, 559
MwoI GCNNNNNNNGC 4 cut(s) 79, 169, 261, 568
NciI CCSGG 3 cut(s) 70, 206, 304
NcoI CCATGG 1 cut(s) 51
NdeII GATC 8 cut(s) 37, 55, 309, 934, 1024, 1242, 1328, 1712
NlaIII CATG 4 cut(s) 55, 1100, 1223, 1322
NlaIV GGNNCC 7 cut(s) 495, 651, 1177, 1416, 1512, 1566, 1578
NmeAIII GCCGAG 4 cut(s) 271, 487, 522, 921
NmuCI GTSAC 1 cut(s) 913
NotI GCGGCCGC 1 cut(s) 560
NsbI TGCGCA 1 cut(s) 677
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 1223, 1322
NspV TTCGAA 1 cut(s) 231
PaeI GCATGC 1 cut(s) 1322
PciSI GCTCTTC 1 cut(s) 1083
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 282, 494
PdmI GAANNNNTTC 1 cut(s) 883
PfeI GAWTC 5 cut(s) 389, 1136, 1214, 1282, 1583
PflFI GACNNNGTC 1 cut(s) 478
PfoI TCCNGGA 1 cut(s) 302
PleI GAGTC 9 cut(s) 307, 463, 472, 487, 493, 827, 1092, 1446, 1479
PpsI GAGTC 9 cut(s) 307, 463, 472, 487, 493, 827, 1092, 1446, 1479
Psp6I CCWGG 2 cut(s) 293, 1035
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 2 cut(s) 293, 1035
PspN4I GGNNCC 7 cut(s) 495, 651, 1177, 1416, 1512, 1566, 1578
PspPI GGNCC 7 cut(s) 67, 190, 195, 263, 315, 474, 494
PsuI RGATCY 3 cut(s) 309, 1242, 1328
PsyI GACNNNGTC 1 cut(s) 478
PvuII CAGCTG 1 cut(s) 1460
RsaI GTAC 4 cut(s) 353, 511, 1202, 1483
RsaNI GTAC 4 cut(s) 352, 510, 1201, 1482
RseI CAYNNNNRTG 1 cut(s) 1323
Rsr2I CGGWCCG 2 cut(s) 190, 474
RsrII CGGWCCG 2 cut(s) 190, 474
SalI GTCGAC 1 cut(s) 466
SapI GCTCTTC 1 cut(s) 1083
SaqAI TTAA 7 cut(s) 549, 1005, 1296, 1428, 1499, 1632, 1667
Sau3AI GATC 8 cut(s) 37, 55, 309, 934, 1024, 1242, 1328, 1712
Sau96I GGNCC 7 cut(s) 67, 190, 195, 263, 315, 474, 494
SchI GAGTC 9 cut(s) 308, 463, 473, 488, 494, 827, 1093, 1447, 1480
ScrFI CCNGG 5 cut(s) 70, 206, 295, 304, 1037
SduI GDGCHC 1 cut(s) 1569
SfaNI GCATC 5 cut(s) 13, 397, 608, 980, 1524
SfuI TTCGAA 1 cut(s) 231
SgrAI CRCCGGYG 1 cut(s) 565
SinI GGWCC 4 cut(s) 190, 195, 315, 474
SmiMI CAYNNNNRTG 1 cut(s) 1323
SpeI ACTAGT 1 cut(s) 917
SphI GCATGC 1 cut(s) 1322
Sse9I AATT 4 cut(s) 419, 546, 975, 1551
SspI AATATT 1 cut(s) 865
SspMI CTAG 3 cut(s) 731, 918, 1236
StyD4I CCNGG 5 cut(s) 68, 204, 293, 302, 1035
StyI CCWWGG 2 cut(s) 51, 1393
TaaI ACNGT 5 cut(s) 351, 451, 457, 787, 1486
TaiI ACGT 3 cut(s) 1202, 1296, 1706
TaqI TCGA 8 cut(s) 40, 180, 210, 231, 467, 587, 623, 1527
TaqII GACCGA 2 cut(s) 491, 1287
TasI AATT 4 cut(s) 419, 546, 975, 1551
TatI WGTACW 2 cut(s) 509, 1481
TauI GCSGC 5 cut(s) 87, 293, 562, 565, 951
TfiI GAWTC 5 cut(s) 389, 1136, 1214, 1282, 1583
Tru1I TTAA 7 cut(s) 549, 1005, 1296, 1428, 1499, 1632, 1667
Tru9I TTAA 7 cut(s) 549, 1005, 1296, 1428, 1499, 1632, 1667
TscAI CASTG 1 cut(s) 1657
TseFI GTSAC 1 cut(s) 913
TseI GCWGC 6 cut(s) 79, 157, 160, 678, 945, 1655
Tsp45I GTSAC 1 cut(s) 913
TspDTI ATGAA 7 cut(s) 723, 732, 984, 985, 1149, 1388, 1600
TspGWI ACGGA 1 cut(s) 1604
TspRI CASTG 1 cut(s) 1657
Tth111I GACNNNGTC 1 cut(s) 478
VpaK11BI GGWCC 4 cut(s) 190, 195, 315, 474
XapI RAATTY 1 cut(s) 975
XceI RCATGY 2 cut(s) 1223, 1322
XcmI CCANNNNNNNNNTGG 1 cut(s) 1464
XmiI GTMKAC 2 cut(s) 467, 1230
XmnI GAANNNNTTC 1 cut(s) 883
XspI CTAG 3 cut(s) 731, 918, 1236
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.