Rh5DG038200

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000243
Physical Location & Seq
Forward (+)
2822869 .. 2823537
669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG038200.1

Sequence Viewer

Length: 498 bp
ATGTATAATAGTTTAAAAACTCTCCTTCTCCACCCACCTCTCTCCTCCTCTCCTCTTCTTCTTCCTCTGTGCATTCCCAGTTTGCCCGTGGTAAATGAAGGAAAGGTCAGTGGTAAATGTAGAATCTCTCTTCTCTCTTCTTCCCCGAACCCCCAAAAAACTCCCGATTTTCCGGCTCGATTTTCCCGGCAAAAATCGACAGCCGCAGCAGCTCCGTCGCTCGACCTGTCGGACCGGACCAGCTTCCGGGTCGAAGGAAATGACGGCGAGTTCGAACGGATTTGCGCCGAGCTGGGCTTCTCCGGCCCAGACAACTTGGCGATACCCGAGGCGGCCTGGGAGTCCCGGAAGATGCGGTCCACCAACTCCGATGTTCTCCCCCTGTCAAAACTGTACGTGATGGATAGTCCGAGACCCGACCCGAAGGACGAATCGGAGTATGAGGATGATGCCGTGGGTAGCGAGGCTGGTTTGGGCTGTAGGCGGAGGCGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

165

Amino Acids

18.08

Weight (kDa)

6.6

Isoelectric Point (pI)

58.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 204, 332, 355, 484, 490
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 1 cut(s) 246
AjnI CCWGG 1 cut(s) 335
AluBI AGCT 3 cut(s) 212, 243, 292
AluI AGCT 3 cut(s) 212, 243, 292
Alw26I GTCTC 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 2 cut(s) 304, 333
ApeKI GCWGC 2 cut(s) 206, 209
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 4 cut(s) 232, 237, 305, 357
AsuC2I CCSGG 3 cut(s) 187, 248, 346
AsuII TTCGAA 1 cut(s) 273
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 3 cut(s) 232, 237, 357
BbvI GCAGC 2 cut(s) 218, 221
BccI CCATC 1 cut(s) 394
BceAI ACGGC 2 cut(s) 280, 437
BcgI CGANNNNNNTGC 2 cut(s) 198, 232
BciT130I CCWGG 1 cut(s) 337
BcnI CCSGG 3 cut(s) 187, 248, 346
BcoDI GTCTC 1 cut(s) 406
BfmI CTRYAG 1 cut(s) 478
BisI GCNGC 4 cut(s) 204, 207, 210, 333
BlsI GCNGC 4 cut(s) 205, 208, 211, 334
Bme1390I CCNGG 4 cut(s) 187, 248, 337, 346
Bme18I GGWCC 3 cut(s) 232, 237, 357
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 4 cut(s) 232, 237, 305, 357
BmrFI CCNGG 4 cut(s) 187, 248, 337, 346
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 2 cut(s) 342, 439
BmuI ACTGGG 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 273
BpuMI CCSGG 3 cut(s) 187, 248, 346
BsaAI YACGTR 1 cut(s) 397
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 4 cut(s) 87, 327, 336, 453
BsaWI WCCGGW 1 cut(s) 234
Bsc4I CCNNNNNNNGG 1 cut(s) 246
Bse1I ACTGG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 4 cut(s) 87, 327, 336, 453
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 78
BseRI GAGGAG 3 cut(s) 34, 37, 42
BseXI GCAGC 2 cut(s) 218, 221
BseYI CCCAGC 1 cut(s) 292
BshFI GGCC 2 cut(s) 306, 335
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 6 cut(s) 173, 187, 235, 247, 303, 346
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 246
BsmAI GTCTC 1 cut(s) 406
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 72
BsnI GGCC 2 cut(s) 306, 335
Bso31I GGTCTC 1 cut(s) 406
BsoBI CYCGRG 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 273
BspACI CCGC 5 cut(s) 204, 332, 355, 484, 490
BspANI GGCC 2 cut(s) 306, 335
BspT104I TTCGAA 1 cut(s) 273
BspTNI GGTCTC 1 cut(s) 406
BsrI ACTGG 1 cut(s) 78
BssECI CCNNGG 4 cut(s) 87, 327, 336, 453
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 393
Bst6I CTCTTC 3 cut(s) 60, 135, 142
BstBAI YACGTR 1 cut(s) 397
BstBI TTCGAA 1 cut(s) 273
BstDSI CCRYGG 2 cut(s) 87, 453
BstF5I GGATG 1 cut(s) 451
BstHHI GCGC 1 cut(s) 287
BstMAI GTCTC 1 cut(s) 406
BstMWI GCNNNNNNNGC 2 cut(s) 209, 303
BstNI CCWGG 1 cut(s) 337
BstSCI CCNGG 4 cut(s) 185, 246, 335, 344
BstSFI CTRYAG 1 cut(s) 478
BstV1I GCAGC 2 cut(s) 218, 221
BsuRI GGCC 2 cut(s) 306, 335
BtgI CCRYGG 2 cut(s) 87, 453
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 1 cut(s) 115
CfoI GCGC 1 cut(s) 287
Cfr13I GGNCC 4 cut(s) 232, 237, 305, 357
CpoI CGGWCCG 1 cut(s) 232
Csp6I GTAC 1 cut(s) 394
CspI CGGWCCG 1 cut(s) 232
CviQI GTAC 1 cut(s) 394
DraI TTTAAA 1 cut(s) 15
Eam1104I CTCTTC 3 cut(s) 60, 135, 142
EarI CTCTTC 3 cut(s) 60, 135, 142
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 3 cut(s) 232, 237, 357
Eco88I CYCGRG 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 335
FaiI YATR 2 cut(s) 6, 441
FaqI GGGAC 1 cut(s) 328
Fnu4HI GCNGC 4 cut(s) 204, 207, 210, 333
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 4 cut(s) 204, 207, 210, 333
GlaI GCGC 1 cut(s) 286
GluI GCNGC 4 cut(s) 204, 207, 210, 333
GsaI CCCAGC 1 cut(s) 296
HaeIII GGCC 2 cut(s) 306, 335
HapII CCGG 6 cut(s) 173, 187, 235, 247, 303, 346
HhaI GCGC 1 cut(s) 287
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 3 cut(s) 123, 341, 431
HpaII CCGG 6 cut(s) 173, 187, 235, 247, 303, 346
Hpy166II GTNNAC 1 cut(s) 360
Hpy188I TCNGA 4 cut(s) 232, 370, 411, 436
Hpy188III TCNNGA 1 cut(s) 164
Hpy8I GTNNAC 1 cut(s) 360
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 4 cut(s) 35, 92, 248, 418
HpyCH4III ACNGT 1 cut(s) 393
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 303
HpySE526I ACGT 1 cut(s) 396
HspAI GCGC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 217
Lsp1109I GCAGC 2 cut(s) 218, 221
LweI GCATC 2 cut(s) 342, 439
MaeII ACGT 1 cut(s) 396
MboII GAAGA 7 cut(s) 47, 50, 53, 122, 129, 132, 361
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 210
MseI TTAA 1 cut(s) 14
MspI CCGG 6 cut(s) 173, 187, 235, 247, 303, 346
MspR9I CCNGG 4 cut(s) 187, 248, 337, 346
Mva1269I GAATGC 1 cut(s) 72
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 2 cut(s) 209, 303
NciI CCSGG 3 cut(s) 187, 248, 346
NmeAIII GCCGAG 1 cut(s) 313
NspV TTCGAA 1 cut(s) 273
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 1 cut(s) 72
PfeI GAWTC 2 cut(s) 123, 431
PfoI TCCNGGA 1 cut(s) 344
PkrI GCNGC 4 cut(s) 205, 208, 211, 334
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
Ppu21I YACGTR 1 cut(s) 397
Psp6I CCWGG 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 1 cut(s) 335
PspPI GGNCC 4 cut(s) 232, 237, 305, 357
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
Rsr2I CGGWCCG 1 cut(s) 232
RsrII CGGWCCG 1 cut(s) 232
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 4 cut(s) 204, 207, 210, 333
Sau96I GGNCC 4 cut(s) 232, 237, 305, 357
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 4 cut(s) 187, 248, 337, 346
SetI ASST 8 cut(s) 40, 108, 214, 228, 245, 294, 399, 497
SfaNI GCATC 2 cut(s) 342, 439
SfcI CTRYAG 1 cut(s) 478
SfuI TTCGAA 1 cut(s) 273
SinI GGWCC 3 cut(s) 232, 237, 357
SsiI CCGC 5 cut(s) 204, 332, 355, 484, 490
StyD4I CCNGG 4 cut(s) 185, 246, 335, 344
TaaI ACNGT 1 cut(s) 393
TaiI ACGT 1 cut(s) 399
TaqI TCGA 5 cut(s) 178, 197, 222, 252, 273
TauI GCSGC 2 cut(s) 206, 335
TfiI GAWTC 2 cut(s) 123, 431
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 1 cut(s) 115
TseI GCWGC 2 cut(s) 206, 209
TspDTI ATGAA 1 cut(s) 111
TspGWI ACGGA 2 cut(s) 204, 292
TspRI CASTG 1 cut(s) 115
VpaK11BI GGWCC 3 cut(s) 232, 237, 357
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.