Rw5G048440

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000243
Physical Location & Seq
Reverse (-)
84222290 .. 84229273
6984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048440.1

Sequence Viewer

Length: 2037 bp
ATGGCCTCGATCCCTCGAAAAACTGATGCGTCTCTTCCTTCATCAGCAGCTCGTCGCTGGAAGTATTACGTGTATTTGAGTTTCAGGGGTGAAGATACTCGAAGGGGTTTTACATCTCAACTATACGACGGGCTGCAGAGGAGGGGAGTTAAAACATTCATGGATCATCATGATCTTCAAGTAGGGGATGATGTTAATGCCACTCTCCTAAAGGCAATTGAAGGATCAAGGTTGGCAATTGTTGTTCTGTCCCAAAATTATGCTTCTTCTGCTTGGTGTTTGGAGGAACTTACAAAGATTTGGCAGTTCATGGAGGACGGCCCCAGAATTCTTCCAGTTTTTTATGATGTCGAGCCTAGTGATGTTAGACATCAAAAGGGGAGTTTTCAAAAAGCTTTCGCCAGGCATGAAATGTCTGGGCGATACGAACCGGAGAAGGTCCAGCTGTGGAGAGATGCTTTAAGAAGAACGGCCAATTTGGCTGGGTGGAGTATCAGGAGTTACAGCACTGAGAGTAAATGTGTCGAAGCCATTGTGGAATCTGTGTACAGTAAACTACAACCTTTTGCGGAAATCAACACGGCAGATTTTGATGATTTTGAACCATCATCAGCAGCTGGTTGGTTGAAGTATGACGTGTATTTGAGTTTCAGGGGTTCAGATACTCGAAGGGGTTTTACATCCCATTTATACGATCGGCTACAAAAGAGAGGAATCAATGCATTCATGGACTCAGATCTTGCAGCAGGAGATGCTATTGGTCAGGCTCTTTTTGGGGCACTTGAAGGATCAAGGTTCGCAATTGTTGTTCTGTCCCCAACTTATGCTTTTTCTACTTGGTGTTTGGAGGAACTTACAAAGATTTGTCAGTTCATGGAGGACGGCCCCAGAATTCTTCCAGTTTTTTATGATGTCCAGCCTAGTGATGTTAGATATCAAAGGGGGAGTTTTCAAAAAGCTTTCACCGGGCATGAAGTGTCTGGGCGATTCGAACCGGAGAAGGTCCGGGAGTGGAGAGATGCTTTAAGAAGAATTACCGATATTTCTGGGTGGGATACAAGGGATTACGACACTGAGACAGGAGTTGTCGAAGCCATTGTGGAATCTGTGTACAGTAAACTACAACCTGTTGTGGTATCCCAGAGCCCTGACTTGACAAAAATTCAAGGCACATTGGCGGGGCCGAGCGGGTATTCTACTGGGAAGCCTAAGATCATCACTAAATGGGAAAAGGTTCGTCATCTCGGCAGCGGCTCTTTCGGGTCTGTGTATGAAGTATTTGCTGATTGCGGAATCTTCTTTGCTGTCAAGGAAGTTTCCTTGCATGATCAAGGAAGAGTTTCTCGGCTTGAACAGGAGATTGCTCTTCTCAGTCAGTTTCAACATGAGAACATAGTTCAGTATTATAGCACACAAAAGGATGAATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAAATGCACCCTCTTACAGATTCGCATGTCTCTGTATACACGAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCAAACTGTGATTCACAGGGACATTAAATGCGCAAATATTTTGGTGCATGCTAATGGATCTGTCAAGCTTGCAGACTTTGGATTGGCAAAGGCTATCAAAATGAACGACAAACTATCTTGCCAAGGAACTGCAAACTGGATGGCCCCTGAGCTTGCTAAACCTGGAAATCCAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGAAGGTTCCATACCCTGATCTCGAATGGATGGCGGCATTATGGAAAATTATGAAGGGGGAGCCCCCTCTGGTTCCTGATTCTCTTTCAAGGGAGGCACAAGATTTTATCCATCTATGCTTACAAGTTGAGCCAGATAATCGTCCCACTGCTGCTCAACTTTTAAAACATCCATTTGTAAATAAGCCCCTTCCTCCCACGTCTTCTGGATCATTATCTCCTTACAATCACCACAGGCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

678

Amino Acids

76.59

Weight (kDa)

6.67

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 192 6.7e-49 TIR domain
TIR_2 PF13676 24 - 120 1.2e-18 TIR domain
TIR PF01582 210 - 381 3.7e-44 TIR domain
TIR_2 PF13676 213 - 307 8.9e-16 TIR domain
Pkinase PF00069 409 - 657 8.4e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 412 - 653 4.4e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1591
AccB7I CCANNNNNTGG 1 cut(s) 1736
AccBSI CCGCTC 1 cut(s) 1188
AccI GTMKAC 1 cut(s) 1518
AciI CCGC 6 cut(s) 569, 1178, 1188, 1251, 1290, 1826
AclWI GGATC 6 cut(s) 4, 171, 232, 796, 1624, 2008
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 3 cut(s) 327, 891, 1161
AcuI CTGAAG 1 cut(s) 1565
AfaI GTAC 3 cut(s) 548, 1112, 1771
AfiI CCNNNNNNNGG 1 cut(s) 1736
AflIII ACRYGT 2 cut(s) 69, 636
AjiI CACGTC 2 cut(s) 637, 1992
AjnI CCWGG 3 cut(s) 401, 1720, 1729
AjuI GAANNNNNNNTTGG 2 cut(s) 1554, 1586
Alw26I GTCTC 4 cut(s) 36, 1070, 1516, 1519
AlwI GGATC 6 cut(s) 4, 171, 232, 796, 1624, 2008
AlwNI CAGNNNCTG 1 cut(s) 617
AoxI GGCC 7 cut(s) 3, 319, 471, 883, 1181, 1701, 1738
ApeKI GCWGC 6 cut(s) 47, 133, 614, 743, 1248, 1943
ApoI RAATTY 3 cut(s) 327, 891, 1161
Asp700I GAANNNNTTC 2 cut(s) 1233, 1339
AspLEI GCGC 1 cut(s) 1592
AspS9I GGNCC 6 cut(s) 320, 439, 884, 1003, 1181, 1702
AsuC2I CCSGG 2 cut(s) 967, 1007
AsuHPI GGTGA 3 cut(s) 101, 955, 2012
AsuII TTCGAA 1 cut(s) 990
AvaII GGWCC 2 cut(s) 439, 1003
BaeGI GKGCMC 1 cut(s) 781
BanII GRGCYC 2 cut(s) 1148, 1857
BarI GAAGNNNNNNTAC 6 cut(s) 94, 126, 661, 693, 1787, 1819
BauI CACGAG 1 cut(s) 1522
BbsI GAAGAC 1 cut(s) 1986
BbvI GCAGC 6 cut(s) 59, 120, 626, 755, 1260, 1930
BccI CCATC 4 cut(s) 613, 1693, 1816, 1911
BceAI ACGGC 4 cut(s) 334, 486, 597, 898
BciT130I CCWGG 3 cut(s) 403, 1722, 1731
BciVI GTATCC 2 cut(s) 1048, 1147
BclI TGATCA 1 cut(s) 1327
BcnI CCSGG 2 cut(s) 967, 1007
BcoDI GTCTC 4 cut(s) 36, 1070, 1516, 1519
BfaI CTAG 2 cut(s) 357, 921
BfmI CTRYAG 1 cut(s) 134
BfuI GTATCC 2 cut(s) 1048, 1147
BglI GCCNNNNNGGC 1 cut(s) 479
BglII AGATCT 2 cut(s) 736, 1530
BisI GCNGC 8 cut(s) 48, 134, 615, 744, 1249, 1252, 1827, 1944
BlsI GCNGC 8 cut(s) 49, 135, 616, 745, 1250, 1253, 1828, 1945
Bme1390I CCNGG 5 cut(s) 403, 967, 1007, 1722, 1731
Bme18I GGWCC 2 cut(s) 439, 1003
BmgBI CACGTC 2 cut(s) 637, 1992
BmgT120I GGNCC 6 cut(s) 320, 439, 884, 1003, 1181, 1702
BmiI GGNNCC 8 cut(s) 322, 886, 1182, 1465, 1704, 1800, 1854, 1866
BmrFI CCNGG 5 cut(s) 403, 967, 1007, 1722, 1731
BmrI ACTGGG 2 cut(s) 1209, 1800
BmsI GCATC 4 cut(s) 16, 445, 742, 1009
BmuI ACTGGG 2 cut(s) 1209, 1800
BpiI GAAGAC 1 cut(s) 1986
Bpu10I CCTNAGC 1 cut(s) 1707
Bpu14I TTCGAA 1 cut(s) 990
BpuMI CCSGG 2 cut(s) 967, 1007
BsaAI YACGTR 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 2005
BsaJI CCNNGG 1 cut(s) 1681
BsaWI WCCGGW 2 cut(s) 430, 994
Bsc4I CCNNNNNNNGG 1 cut(s) 1736
Bse1I ACTGG 5 cut(s) 335, 899, 1204, 1700, 1795
Bse8I GATNNNNATC 1 cut(s) 2005
BseBI CCWGG 3 cut(s) 403, 1722, 1731
BseDI CCNNGG 1 cut(s) 1681
BseGI GGATG 7 cut(s) 193, 680, 1426, 1704, 1772, 1827, 1960
BseJI GATNNNNATC 1 cut(s) 2005
BseLI CCNNNNNNNGG 1 cut(s) 1736
BseMII CTCAG 5 cut(s) 501, 747, 1065, 1384, 1698
BseNI ACTGG 5 cut(s) 335, 899, 1204, 1700, 1795
BseRI GAGGAG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 781
BseXI GCAGC 6 cut(s) 59, 120, 626, 755, 1260, 1930
BseYI CCCAGC 1 cut(s) 482
Bsh1285I CGRYCG 1 cut(s) 697
BshFI GGCC 7 cut(s) 5, 321, 473, 885, 1183, 1703, 1740
BsiEI CGRYCG 1 cut(s) 697
BsiSI CCGG 4 cut(s) 431, 966, 995, 1006
BslFI GGGAC 4 cut(s) 235, 799, 1592, 1920
BslI CCNNNNNNNGG 1 cut(s) 1736
BsmAI GTCTC 4 cut(s) 36, 1070, 1516, 1519
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 4 cut(s) 235, 799, 1592, 1920
BsmI GAATGC 1 cut(s) 722
BsnI GGCC 7 cut(s) 5, 321, 473, 885, 1183, 1703, 1740
Bsp119I TTCGAA 1 cut(s) 990
Bsp1286I GDGCHC 3 cut(s) 781, 1148, 1857
Bsp1407I TGTACA 2 cut(s) 546, 1110
BspACI CCGC 6 cut(s) 569, 1178, 1188, 1251, 1290, 1826
BspANI GGCC 7 cut(s) 5, 321, 473, 885, 1183, 1703, 1740
BspCNI CTCAG 5 cut(s) 502, 746, 1066, 1383, 1699
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 8 cut(s) 322, 886, 1182, 1465, 1704, 1800, 1854, 1866
BspMAI CTGCAG 1 cut(s) 138
BspPI GGATC 6 cut(s) 4, 171, 232, 796, 1624, 2008
BspQI GCTCTTC 1 cut(s) 1371
BspT104I TTCGAA 1 cut(s) 990
BsrBI CCGCTC 1 cut(s) 1188
BsrGI TGTACA 2 cut(s) 546, 1110
BsrI ACTGG 5 cut(s) 335, 899, 1204, 1700, 1795
BssECI CCNNGG 1 cut(s) 1681
BssNAI GTATAC 1 cut(s) 1519
BssSI CACGAG 1 cut(s) 1522
BssT1I CCWWGG 1 cut(s) 1681
Bst1107I GTATAC 1 cut(s) 1519
Bst2BI CACGAG 1 cut(s) 1522
Bst2UI CCWGG 3 cut(s) 403, 1722, 1731
Bst4CI ACNGT 4 cut(s) 551, 1115, 1567, 1774
Bst6I CTCTTC 3 cut(s) 39, 1330, 1371
BstAPI GCANNNNNTGC 1 cut(s) 752
BstAUI TGTACA 2 cut(s) 546, 1110
BstBAI YACGTR 1 cut(s) 70
BstBI TTCGAA 1 cut(s) 990
BstC8I GCNNGC 3 cut(s) 1608, 1629, 1713
BstDEI CTNAG 6 cut(s) 510, 733, 1074, 1209, 1370, 1707
BstF5I GGATG 7 cut(s) 193, 680, 1426, 1704, 1772, 1827, 1960
BstHHI GCGC 1 cut(s) 1592
BstMAI GTCTC 4 cut(s) 36, 1070, 1516, 1519
BstMCI CGRYCG 1 cut(s) 697
BstMWI GCNNNNNNNGC 3 cut(s) 269, 479, 752
BstNI CCWGG 3 cut(s) 403, 1722, 1731
BstNSI RCATGY 2 cut(s) 1511, 1610
BstSCI CCNGG 5 cut(s) 401, 965, 1005, 1720, 1729
BstSFI CTRYAG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 781
BstV1I GCAGC 6 cut(s) 59, 120, 626, 755, 1260, 1930
BstV2I GAAGAC 1 cut(s) 1986
BstX2I RGATCY 3 cut(s) 736, 1530, 1616
BstYI RGATCY 3 cut(s) 736, 1530, 1616
BstZ17I GTATAC 1 cut(s) 1519
BsuI GTATCC 2 cut(s) 1048, 1147
BsuRI GGCC 7 cut(s) 5, 321, 473, 885, 1183, 1703, 1740
BtrI CACGTC 2 cut(s) 637, 1992
BtsCI GGATG 7 cut(s) 193, 680, 1426, 1704, 1772, 1827, 1960
BtsI GCAGTG 1 cut(s) 1938
BtsIMutI CAGTG 3 cut(s) 507, 1071, 1938
Cac8I GCNNGC 3 cut(s) 1608, 1629, 1713
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 1 cut(s) 169
CfoI GCGC 1 cut(s) 1592
Cfr13I GGNCC 6 cut(s) 320, 439, 884, 1003, 1181, 1702
CseI GACGC 1 cut(s) 18
Csp6I GTAC 3 cut(s) 547, 1111, 1770
CviQI GTAC 3 cut(s) 547, 1111, 1770
DdeI CTNAG 6 cut(s) 510, 733, 1074, 1209, 1370, 1707
DraI TTTAAA 1 cut(s) 1956
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 3 cut(s) 39, 1330, 1371
EarI CTCTTC 3 cut(s) 39, 1330, 1371
Eco130I CCWWGG 1 cut(s) 1681
Eco24I GRGCYC 2 cut(s) 1148, 1857
Eco32I GATATC 1 cut(s) 935
Eco47I GGWCC 2 cut(s) 439, 1003
Eco57I CTGAAG 1 cut(s) 1565
EcoRI GAATTC 2 cut(s) 327, 891
EcoRII CCWGG 3 cut(s) 401, 1720, 1729
EcoRV GATATC 1 cut(s) 935
EcoT14I CCWWGG 1 cut(s) 1681
EcoT22I ATGCAT 1 cut(s) 724
EcoT38I GRGCYC 2 cut(s) 1148, 1857
ErhI CCWWGG 1 cut(s) 1681
Esp3I CGTCTC 1 cut(s) 36
FalI AAGNNNNNCTT 2 cut(s) 1452, 1484
FaqI GGGAC 4 cut(s) 235, 799, 1592, 1920
FauI CCCGC 2 cut(s) 1171, 1181
FbaI TGATCA 1 cut(s) 1327
FblI GTMKAC 1 cut(s) 1518
Fnu4HI GCNGC 8 cut(s) 48, 134, 615, 744, 1249, 1252, 1827, 1944
FokI GGATG 7 cut(s) 200, 667, 1433, 1711, 1779, 1834, 1947
FriOI GRGCYC 2 cut(s) 1148, 1857
Fsp4HI GCNGC 8 cut(s) 48, 134, 615, 744, 1249, 1252, 1827, 1944
FspBI CTAG 2 cut(s) 357, 921
FspI TGCGCA 1 cut(s) 1591
GlaI GCGC 1 cut(s) 1591
GluI GCNGC 8 cut(s) 48, 134, 615, 744, 1249, 1252, 1827, 1944
GsaI CCCAGC 1 cut(s) 486
HaeIII GGCC 7 cut(s) 5, 321, 473, 885, 1183, 1703, 1740
HapII CCGG 4 cut(s) 431, 966, 995, 1006
HgaI GACGC 1 cut(s) 18
HhaI GCGC 1 cut(s) 1592
Hin6I GCGC 1 cut(s) 1590
HinP1I GCGC 1 cut(s) 1590
HincII GTYRAC 1 cut(s) 1788
HindII GTYRAC 1 cut(s) 1788
HindIII AAGCTT 3 cut(s) 393, 957, 1625
HpaI GTTAAC 1 cut(s) 1788
HpaII CCGG 4 cut(s) 431, 966, 995, 1006
HphI GGTGA 3 cut(s) 101, 955, 2012
Hpy166II GTNNAC 6 cut(s) 547, 554, 1111, 1118, 1519, 1788
Hpy188I TCNGA 3 cut(s) 661, 736, 1545
Hpy188III TCNNGA 7 cut(s) 170, 496, 1445, 1556, 1814, 1868, 1998
Hpy8I GTNNAC 6 cut(s) 547, 554, 1111, 1118, 1519, 1788
Hpy99I CGWCG 2 cut(s) 57, 131
HpyCH4III ACNGT 4 cut(s) 551, 1115, 1567, 1774
HpyCH4IV ACGT 3 cut(s) 69, 636, 1991
HpyCH4V TGCA 9 cut(s) 136, 722, 743, 1324, 1489, 1537, 1606, 1631, 1691
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 479, 752
HpyF3I CTNAG 6 cut(s) 510, 733, 1074, 1209, 1370, 1707
HpySE526I ACGT 3 cut(s) 69, 636, 1991
HspAI GCGC 1 cut(s) 1590
Ksp22I TGATCA 1 cut(s) 1327
KspAI GTTAAC 1 cut(s) 1788
LguI GCTCTTC 1 cut(s) 1371
LmnI GCTCC 2 cut(s) 1469, 1852
Lsp1109I GCAGC 6 cut(s) 59, 120, 626, 755, 1260, 1930
LweI GCATC 4 cut(s) 16, 445, 742, 1009
MaeI CTAG 2 cut(s) 357, 921
MaeII ACGT 3 cut(s) 69, 636, 1991
MaeIII GTNAC 2 cut(s) 500, 1453
MbiI CCGCTC 1 cut(s) 1188
MfeI CAATTG 3 cut(s) 216, 237, 801
MflI RGATCY 3 cut(s) 736, 1530, 1616
MhlI GDGCHC 3 cut(s) 781, 1148, 1857
MlyI GAGTC 2 cut(s) 725, 1768
MmeI TCCRAC 1 cut(s) 1758
Mph1103I ATGCAT 1 cut(s) 724
MroXI GAANNNNTTC 2 cut(s) 1233, 1339
MseI TTAA 7 cut(s) 150, 195, 461, 1025, 1584, 1787, 1955
MslI CAYNNNNRTG 1 cut(s) 1611
MspA1I CMGCKG 4 cut(s) 445, 617, 1251, 1748
MspI CCGG 4 cut(s) 431, 966, 995, 1006
MspR9I CCNGG 5 cut(s) 403, 967, 1007, 1722, 1731
MunI CAATTG 3 cut(s) 216, 237, 801
Mva1269I GAATGC 1 cut(s) 722
MvaI CCWGG 3 cut(s) 403, 1722, 1731
MwoI GCNNNNNNNGC 3 cut(s) 269, 479, 752
NciI CCSGG 2 cut(s) 967, 1007
NlaIV GGNNCC 8 cut(s) 322, 886, 1182, 1465, 1704, 1800, 1854, 1866
NmeAIII GCCGAG 3 cut(s) 1209, 1224, 1324
NsbI TGCGCA 1 cut(s) 1591
NsiI ATGCAT 1 cut(s) 724
NspI RCATGY 2 cut(s) 1511, 1610
NspV TTCGAA 1 cut(s) 990
PaeI GCATGC 1 cut(s) 1610
PagI TCATGA 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 1371
PctI GAATGC 1 cut(s) 722
PdmI GAANNNNTTC 2 cut(s) 1233, 1339
PfeI GAWTC 9 cut(s) 539, 714, 987, 1103, 1293, 1424, 1502, 1570, 1871
PflMI CCANNNNNTGG 1 cut(s) 1736
PfoI TCCNGGA 1 cut(s) 1005
PkrI GCNGC 8 cut(s) 49, 135, 616, 745, 1250, 1253, 1828, 1945
Ple19I CGATCG 1 cut(s) 697
PleI GAGTC 2 cut(s) 725, 1767
PpsI GAGTC 2 cut(s) 725, 1767
Ppu21I YACGTR 1 cut(s) 70
Psp6I CCWGG 3 cut(s) 401, 1720, 1729
PspFI CCCAGC 1 cut(s) 482
PspGI CCWGG 3 cut(s) 401, 1720, 1729
PspN4I GGNNCC 8 cut(s) 322, 886, 1182, 1465, 1704, 1800, 1854, 1866
PspPI GGNCC 6 cut(s) 320, 439, 884, 1003, 1181, 1702
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 3 cut(s) 736, 1530, 1616
PvuI CGATCG 1 cut(s) 697
PvuII CAGCTG 3 cut(s) 445, 617, 1748
RsaI GTAC 3 cut(s) 548, 1112, 1771
RsaNI GTAC 3 cut(s) 547, 1111, 1770
RseI CAYNNNNRTG 1 cut(s) 1611
SapI GCTCTTC 1 cut(s) 1371
SaqAI TTAA 7 cut(s) 150, 195, 461, 1025, 1584, 1787, 1955
SatI GCNGC 8 cut(s) 48, 134, 615, 744, 1249, 1252, 1827, 1944
Sau96I GGNCC 6 cut(s) 320, 439, 884, 1003, 1181, 1702
SchI GAGTC 2 cut(s) 725, 1768
ScrFI CCNGG 5 cut(s) 403, 967, 1007, 1722, 1731
SduI GDGCHC 3 cut(s) 781, 1148, 1857
SfaNI GCATC 4 cut(s) 16, 445, 742, 1009
SfcI CTRYAG 1 cut(s) 134
SfuI TTCGAA 1 cut(s) 990
SinI GGWCC 2 cut(s) 439, 1003
SmiMI CAYNNNNRTG 1 cut(s) 1611
SphI GCATGC 1 cut(s) 1610
SsiI CCGC 6 cut(s) 569, 1178, 1188, 1251, 1290, 1826
SspI AATATT 1 cut(s) 1597
SspMI CTAG 2 cut(s) 357, 921
StyD4I CCNGG 5 cut(s) 401, 965, 1005, 1720, 1729
StyI CCWWGG 1 cut(s) 1681
TaaI ACNGT 4 cut(s) 551, 1115, 1567, 1774
TaiI ACGT 3 cut(s) 72, 639, 1994
TaqI TCGA 9 cut(s) 8, 16, 100, 351, 525, 667, 990, 1089, 1815
TatI WGTACW 3 cut(s) 546, 1110, 1769
TauI GCSGC 2 cut(s) 1254, 1829
TfiI GAWTC 9 cut(s) 539, 714, 987, 1103, 1293, 1424, 1502, 1570, 1871
Tru1I TTAA 7 cut(s) 150, 195, 461, 1025, 1584, 1787, 1955
Tru9I TTAA 7 cut(s) 150, 195, 461, 1025, 1584, 1787, 1955
TscAI CASTG 3 cut(s) 514, 1078, 1945
TseI GCWGC 6 cut(s) 47, 133, 614, 743, 1248, 1943
TspRI CASTG 3 cut(s) 514, 1078, 1945
Van91I CCANNNNNTGG 1 cut(s) 1736
VpaK11BI GGWCC 2 cut(s) 439, 1003
XapI RAATTY 3 cut(s) 327, 891, 1161
XceI RCATGY 2 cut(s) 1511, 1610
XcmI CCANNNNNNNNNTGG 1 cut(s) 1752
XmiI GTMKAC 1 cut(s) 1518
XmnI GAANNNNTTC 2 cut(s) 1233, 1339
XspI CTAG 2 cut(s) 357, 921
Zsp2I ATGCAT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.