RLG00000028354

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000243
Physical Location & Seq
Forward (+)
21749761 .. 21754021
4261 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028354

Sequence Viewer

Length: 963 bp
ATGATTCTTGGATCTGGTGACTTCAACTTTGGCCTCCATTCCTCTCCACATTGCATTGACGACCACCCGCAAATGCAAAGAATGAGCCATGAGATGCAATATATTGAATTCCTTGCAAAAATGAATTTTTCTCCTGATAGCAAATGGACAAGAATTCAGCAACTGGGTCGTGGGTCTTCTGGATCTGTCTTCGAAGGATGTTTTGAGAACGGATTCCACTTTGCTGTCAAAGTAGTTTCCTTGTATGATGAAGGAAGTCGAGGGAGGAACTGCGTCAATCAACTTGAACAGGAGATTGCTCTTTGGAAGCGGTTTAAACATCAGAACACAGTTCAATATTATTACTCATATAAGGATGAACTGAACCTTTATATGTTCATGGAGCTTGTACCTCGAGGATCCCTACGAAGCTTCTATCAGACAAATCCCGTTTCCTGTCTTCTCGTCTCCAAATACACAAAAGATATTCTTCATGGTCTTAAGTATCTTCATGACCACAATGTGATTCACAGGGACATAAAATGTGCAAATATATTGATAGATGCTCAAGGATCTGCAAAGCTCTCAGATTTGGAAGTGGGAAAGATAATCAGAATGAATGATATTAAATCTTTCCAGAGCAGTGCATTTTGGACAGCTCCTGAGGTTCTGAGTGCAGTAAAGCATAACCAAGGTTATGGGATTTCAGCTGATATATGGAGCCTTGGTTGCACCGTATTGGAGATGTTAACCAGGCGTGTTCCTTACCATGATATGACACCGGAACAGGCATTATGGAGATCATTGAACGGGAAGCTCCCTGAGATTCCTGATTTTCTCTCAGTGGAAGCACAGGATTTCATCCAGAAATGCCTAAAGATCAACCCAAATGATCGTCCCACTGCTTCAGAGCTCTTAGAGCACCCATTTGTCTTGAGGCGGCCAAGGTTGGGCAACATGCCAAGGCGAGAGAAATGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

321

Amino Acids

36.94

Weight (kDa)

8.07

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 50 - 304 4.4e-61 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 52 - 300 7.1e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 393
AciI CCGC 3 cut(s) 68, 310, 919
AclWI GGATC 5 cut(s) 19, 190, 393, 406, 559
AcoI YGGCCR 1 cut(s) 920
AcsI RAATTY 3 cut(s) 107, 124, 153
AcuI CTGAAG 1 cut(s) 870
AdeI CACNNNGTG 1 cut(s) 502
AfaI GTAC 1 cut(s) 390
AfiI CCNNNNNNNGG 1 cut(s) 929
AflII CTTAAG 1 cut(s) 479
AgsI TTSAA 5 cut(s) 25, 107, 287, 335, 787
AjnI CCWGG 1 cut(s) 731
AluBI AGCT 7 cut(s) 385, 411, 562, 638, 689, 796, 892
AluI AGCT 7 cut(s) 385, 411, 562, 638, 689, 796, 892
Alw21I GWGCWC 2 cut(s) 894, 903
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 5 cut(s) 19, 190, 393, 406, 559
AlwNI CAGNNNCTG 2 cut(s) 163, 641
Ama87I CYCGRG 1 cut(s) 393
AoxI GGCC 2 cut(s) 31, 920
ApoI RAATTY 3 cut(s) 107, 124, 153
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
Asp700I GAANNNNTTC 1 cut(s) 212
AsuHPI GGTGA 1 cut(s) 29
AsuII TTCGAA 1 cut(s) 192
AvaI CYCGRG 1 cut(s) 393
AxyI CCTNAGG 1 cut(s) 642
BamHI GGATCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 894
BbsI GAAGAC 3 cut(s) 168, 181, 431
Bbv12I GWGCWC 2 cut(s) 894, 903
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 1 cut(s) 733
BcoDI GTCTC 1 cut(s) 451
BfrI CTTAAG 1 cut(s) 479
BisI GCNGC 1 cut(s) 920
BlsI GCNGC 1 cut(s) 921
Bme1390I CCNGG 1 cut(s) 733
BmeT110I CYCGRG 1 cut(s) 393
BmiI GGNNCC 2 cut(s) 400, 701
BmrFI CCNGG 1 cut(s) 733
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 2 cut(s) 84, 532
BmuI ACTGGG 1 cut(s) 173
BpiI GAAGAC 3 cut(s) 168, 181, 431
Bpu14I TTCGAA 1 cut(s) 192
BpuEI CTTGAG 2 cut(s) 531, 934
BsaJI CCNNGG 4 cut(s) 670, 703, 923, 941
BsaWI WCCGGW 1 cut(s) 760
BsaXI ACNNNNNCTCC 2 cut(s) 691, 721
Bsc4I CCNNNNNNNGG 1 cut(s) 929
Bse1I ACTGG 1 cut(s) 168
Bse21I CCTNAGG 1 cut(s) 642
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 1 cut(s) 733
BseDI CCNNGG 4 cut(s) 670, 703, 923, 941
BseGI GGATG 3 cut(s) 203, 361, 840
BseLI CCNNNNNNNGG 1 cut(s) 929
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 5 cut(s) 579, 633, 641, 792, 834
BseNI ACTGG 1 cut(s) 168
BsgI GTGCAG 1 cut(s) 675
BshFI GGCC 2 cut(s) 33, 922
BsiHKAI GWGCWC 2 cut(s) 894, 903
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 1 cut(s) 761
BslFI GGGAC 2 cut(s) 527, 861
BslI CCNNNNNNNGG 1 cut(s) 929
BsmAI GTCTC 1 cut(s) 451
BsmBI CGTCTC 1 cut(s) 451
BsmFI GGGAC 2 cut(s) 527, 861
BsnI GGCC 2 cut(s) 33, 922
BsoBI CYCGRG 1 cut(s) 393
Bsp119I TTCGAA 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 894, 903
Bsp143I GATC 7 cut(s) 11, 182, 398, 551, 779, 858, 871
BspACI CCGC 3 cut(s) 68, 310, 919
BspANI GGCC 2 cut(s) 33, 922
BspCNI CTCAG 5 cut(s) 578, 634, 642, 793, 833
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 2 cut(s) 400, 701
BspPI GGATC 5 cut(s) 19, 190, 393, 406, 559
BspT104I TTCGAA 1 cut(s) 192
BspTI CTTAAG 1 cut(s) 479
BsrDI GCAATG 1 cut(s) 49
BsrI ACTGG 1 cut(s) 168
BssECI CCNNGG 4 cut(s) 670, 703, 923, 941
BssMI GATC 7 cut(s) 11, 182, 398, 551, 779, 858, 871
BssT1I CCWWGG 4 cut(s) 670, 703, 923, 941
Bst2UI CCWGG 1 cut(s) 733
Bst4CI ACNGT 2 cut(s) 331, 715
BstAFI CTTAAG 1 cut(s) 479
BstBI TTCGAA 1 cut(s) 192
BstDEI CTNAG 6 cut(s) 565, 642, 650, 801, 820, 895
BstF5I GGATG 3 cut(s) 203, 361, 840
BstKTI GATC 7 cut(s) 14, 185, 401, 554, 782, 861, 874
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 7 cut(s) 11, 182, 398, 551, 779, 858, 871
BstMWI GCNNNNNNNGC 2 cut(s) 708, 898
BstNI CCWGG 1 cut(s) 733
BstNSI RCATGY 1 cut(s) 940
BstSCI CCNGG 1 cut(s) 731
BstV2I GAAGAC 3 cut(s) 168, 181, 431
BstX2I RGATCY 4 cut(s) 11, 182, 398, 551
BstXI CCANNNNNNTGG 1 cut(s) 677
BstYI RGATCY 4 cut(s) 11, 182, 398, 551
Bsu36I CCTNAGG 1 cut(s) 642
BsuRI GGCC 2 cut(s) 33, 922
BtsCI GGATG 3 cut(s) 203, 361, 840
BtsI GCAGTG 2 cut(s) 628, 879
BtsIMutI CAGTG 3 cut(s) 628, 828, 879
CaiI CAGNNNCTG 2 cut(s) 163, 641
CciI TCATGA 1 cut(s) 490
CseI GACGC 1 cut(s) 262
Csp6I GTAC 1 cut(s) 389
CviAII CATG 6 cut(s) 89, 379, 473, 491, 749, 937
CviQI GTAC 1 cut(s) 389
DdeI CTNAG 6 cut(s) 565, 642, 650, 801, 820, 895
DpnI GATC 7 cut(s) 13, 184, 400, 553, 781, 860, 873
DpnII GATC 7 cut(s) 11, 182, 398, 551, 779, 858, 871
DraI TTTAAA 1 cut(s) 316
DraIII CACNNNGTG 1 cut(s) 502
EaeI YGGCCR 1 cut(s) 920
Ecl136II GAGCTC 1 cut(s) 892
Eco130I CCWWGG 4 cut(s) 670, 703, 923, 941
Eco24I GRGCYC 1 cut(s) 894
Eco53kI GAGCTC 1 cut(s) 892
Eco57I CTGAAG 1 cut(s) 870
Eco81I CCTNAGG 1 cut(s) 642
Eco88I CYCGRG 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 892
EcoRI GAATTC 2 cut(s) 107, 153
EcoRII CCWGG 1 cut(s) 731
EcoT14I CCWWGG 4 cut(s) 670, 703, 923, 941
EcoT38I GRGCYC 1 cut(s) 894
ErhI CCWWGG 4 cut(s) 670, 703, 923, 941
Esp3I CGTCTC 1 cut(s) 451
FaeI CATG 6 cut(s) 92, 382, 476, 494, 752, 940
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FaqI GGGAC 2 cut(s) 527, 861
FatI CATG 6 cut(s) 88, 378, 472, 490, 748, 936
FauI CCCGC 1 cut(s) 75
Fnu4HI GCNGC 1 cut(s) 920
FokI GGATG 3 cut(s) 210, 368, 827
FriOI GRGCYC 1 cut(s) 894
Fsp4HI GCNGC 1 cut(s) 920
GluI GCNGC 1 cut(s) 920
HaeIII GGCC 2 cut(s) 33, 922
HapII CCGG 1 cut(s) 761
HgaI GACGC 1 cut(s) 262
Hin1II CATG 6 cut(s) 92, 382, 476, 494, 752, 940
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 409
HinfI GANTC 4 cut(s) 4, 213, 505, 805
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 1 cut(s) 761
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 1 cut(s) 729
Hpy188I TCNGA 6 cut(s) 324, 420, 568, 593, 651, 889
Hpy188III TCNNGA 8 cut(s) 134, 180, 491, 616, 641, 809, 844, 913
Hpy8I GTNNAC 1 cut(s) 729
HpyAV CCTTC 2 cut(s) 188, 245
HpyCH4III ACNGT 2 cut(s) 331, 715
HpyCH4V TGCA 9 cut(s) 54, 76, 97, 116, 527, 557, 626, 656, 711
HpyF10VI GCNNNNNNNGC 2 cut(s) 708, 898
HpyF3I CTNAG 6 cut(s) 565, 642, 650, 801, 820, 895
Hsp92II CATG 6 cut(s) 92, 382, 476, 494, 752, 940
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 7 cut(s) 11, 182, 398, 551, 779, 858, 871
LmnI GCTCC 4 cut(s) 382, 643, 699, 801
LweI GCATC 2 cut(s) 84, 532
MaeIII GTNAC 1 cut(s) 17
MalI GATC 7 cut(s) 13, 184, 400, 553, 781, 860, 873
MboI GATC 7 cut(s) 11, 182, 398, 551, 779, 858, 871
MboII GAAGA 5 cut(s) 168, 181, 431, 461, 479
MflI RGATCY 4 cut(s) 11, 182, 398, 551
MhlI GDGCHC 2 cut(s) 894, 903
MluCI AATT 3 cut(s) 107, 124, 153
MnlI CCTC 8 cut(s) 44, 52, 254, 258, 389, 402, 637, 909
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 4 cut(s) 315, 480, 606, 728
MspA1I CMGCKG 1 cut(s) 689
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 1 cut(s) 761
MspR9I CCNGG 1 cut(s) 733
MssI GTTTAAAC 1 cut(s) 316
MvaI CCWGG 1 cut(s) 733
MwoI GCNNNNNNNGC 2 cut(s) 708, 898
NdeII GATC 7 cut(s) 11, 182, 398, 551, 779, 858, 871
NlaIII CATG 6 cut(s) 92, 382, 476, 494, 752, 940
NlaIV GGNNCC 2 cut(s) 400, 701
NmuCI GTSAC 1 cut(s) 17
NspI RCATGY 1 cut(s) 940
NspV TTCGAA 1 cut(s) 192
PaeR7I CTCGAG 1 cut(s) 393
PagI TCATGA 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 4 cut(s) 4, 213, 505, 805
PkrI GCNGC 1 cut(s) 921
PmeI GTTTAAAC 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 894
Psp6I CCWGG 1 cut(s) 731
PspGI CCWGG 1 cut(s) 731
PspN4I GGNNCC 2 cut(s) 400, 701
PspXI VCTCGAGB 1 cut(s) 393
PstNI CAGNNNCTG 2 cut(s) 163, 641
PsuI RGATCY 4 cut(s) 11, 182, 398, 551
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 1 cut(s) 390
RsaNI GTAC 1 cut(s) 389
SacI GAGCTC 1 cut(s) 894
SaqAI TTAA 4 cut(s) 315, 480, 606, 728
SatI GCNGC 1 cut(s) 920
Sau3AI GATC 7 cut(s) 11, 182, 398, 551, 779, 858, 871
ScrFI CCNGG 1 cut(s) 733
SduI GDGCHC 2 cut(s) 894, 903
SfaNI GCATC 2 cut(s) 84, 532
Sfr274I CTCGAG 1 cut(s) 393
SfuI TTCGAA 1 cut(s) 192
SlaI CTCGAG 1 cut(s) 393
SmlI CTYRAG 4 cut(s) 393, 479, 546, 913
SmoI CTYRAG 4 cut(s) 393, 479, 546, 913
Sse9I AATT 3 cut(s) 107, 124, 153
SsiI CCGC 3 cut(s) 68, 310, 919
SspI AATATT 1 cut(s) 338
SstI GAGCTC 1 cut(s) 894
StyD4I CCNGG 1 cut(s) 731
StyI CCWWGG 4 cut(s) 670, 703, 923, 941
TaaI ACNGT 2 cut(s) 331, 715
TaqI TCGA 3 cut(s) 192, 259, 394
TasI AATT 3 cut(s) 107, 124, 153
TauI GCSGC 1 cut(s) 922
TfiI GAWTC 4 cut(s) 4, 213, 505, 805
Tru1I TTAA 4 cut(s) 315, 480, 606, 728
Tru9I TTAA 4 cut(s) 315, 480, 606, 728
TscAI CASTG 3 cut(s) 628, 828, 886
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 8 cut(s) 137, 264, 367, 372, 461, 479, 611, 829
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 3 cut(s) 628, 828, 886
Vha464I CTTAAG 1 cut(s) 479
XapI RAATTY 3 cut(s) 107, 124, 153
XceI RCATGY 1 cut(s) 940
XhoI CTCGAG 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.