Rh6BG365200

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000243
Physical Location & Seq
Reverse (-)
59434347 .. 59437172
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG365200.1

Sequence Viewer

Length: 711 bp
ATGGTTCTGGACTTGTTCAAGGAACTAGATGGATTCATTTTCGCTGCCAAGGAAGTTTCATTGCTTGATCAAGGAAGTCAGGGGAGGAGCTGTGTTTCTCAACTTCAACAGGAGATTGAGTTTCTCAGTTGGCTTGAACACAAAAACATAGTTCAATACTATGGCTCATTCGAGTATGGATCAAATCTTTATATGTGTATGGAGCATGCACCTAATGGATCCATTCGACAACTCTATCAGAGATCGAATCTTTCACTTCCTCGTGTCTCTAGATACACAAAAGAGATCATTCTTGGTCTAAAGTATCTTCATGACCATAATGTGGTTCACAGGGACATTGAATGTGCAAATATGCTAGAATATTTTTGTTTACACTTATTCTCATCCATTTATTTCTTGTACAAAGGGATCAAATGTGCAAATATTTTAGTGGATGCTTATAGATCTGCAAAGCTTGCAGATTTTGGATTGGCAAAGGGGACTGCATTCTGGATGGCCCCTGAGGTTTTTAGTGCAAAAATCAAGAACCAAGGTTATGGACCTCCAGCGGATATATGGAGCCTTGGTTGCACTGTGTTGGAGATGTTAACAAGGCAGGTTCCCTACCCTGGTTTGGAACCGTTTCAGGCATGTTTTAAGATTGCAATGGGGGAGCTCCCTCACATTCCTCATTTTCTTTGTAAGGAGGTGCGAGATTTGATCCATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

236

Amino Acids

27.09

Weight (kDa)

6.5

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 119 2.1e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 32 - 119 2e-15 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 129 - 235 8e-11 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 131 - 206 6e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 586
AccB7I CCANNNNNTGG 1 cut(s) 322
AciI CCGC 1 cut(s) 548
AclWI GGATC 5 cut(s) 187, 213, 226, 416, 694
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 3 cut(s) 322, 608, 613
AgsI TTSAA 5 cut(s) 19, 107, 137, 155, 341
AjnI CCWGG 1 cut(s) 607
AluBI AGCT 3 cut(s) 90, 454, 655
AluI AGCT 3 cut(s) 90, 454, 655
Alw21I GWGCWC 1 cut(s) 657
Alw26I GTCTC 1 cut(s) 271
AlwI GGATC 5 cut(s) 187, 213, 226, 416, 694
AoxI GGCC 1 cut(s) 495
ApeKI GCWGC 1 cut(s) 44
Asp700I GAANNNNTTC 1 cut(s) 621
AspS9I GGNCC 2 cut(s) 496, 539
AvaII GGWCC 1 cut(s) 539
AxyI CCTNAGG 1 cut(s) 501
BamHI GGATCC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 657
BauI CACGAG 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 657
BbvI GCAGC 1 cut(s) 31
BccI CCATC 2 cut(s) 23, 487
BciT130I CCWGG 1 cut(s) 609
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 3 cut(s) 26, 270, 356
BfuAI ACCTGC 1 cut(s) 586
BglII AGATCT 1 cut(s) 443
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
Bme1390I CCNGG 1 cut(s) 609
Bme18I GGWCC 1 cut(s) 539
BmgT120I GGNCC 2 cut(s) 496, 539
BmiI GGNNCC 5 cut(s) 220, 498, 560, 600, 618
BmrFI CCNGG 1 cut(s) 609
BmsI GCATC 1 cut(s) 424
BpmI CTGGAG 1 cut(s) 528
BsaJI CCNNGG 4 cut(s) 48, 529, 562, 607
BsaXI ACNNNNNCTCC 6 cut(s) 76, 104, 106, 134, 550, 580
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 608, 613
Bse21I CCTNAGG 1 cut(s) 501
Bse3DI GCAATG 2 cut(s) 59, 651
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 4 cut(s) 48, 529, 562, 607
BseGI GGATG 3 cut(s) 383, 439, 498
BseLI CCNNNNNNNGG 3 cut(s) 322, 608, 613
BseMI GCAATG 2 cut(s) 59, 651
BseMII CTCAG 2 cut(s) 139, 492
BseRI GAGGAG 1 cut(s) 100
BseXI GCAGC 1 cut(s) 31
BshFI GGCC 1 cut(s) 497
BsiHKAI GWGCWC 1 cut(s) 657
BslFI GGGAC 2 cut(s) 347, 493
BslI CCNNNNNNNGG 3 cut(s) 322, 608, 613
BsmAI GTCTC 1 cut(s) 271
BsmFI GGGAC 2 cut(s) 347, 493
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 1 cut(s) 497
Bsp1286I GDGCHC 1 cut(s) 657
Bsp1407I TGTACA 1 cut(s) 399
Bsp143I GATC 8 cut(s) 67, 179, 218, 242, 285, 408, 443, 699
BspACI CCGC 1 cut(s) 548
BspANI GGCC 1 cut(s) 497
BspCNI CTCAG 2 cut(s) 138, 493
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 5 cut(s) 220, 498, 560, 600, 618
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 5 cut(s) 187, 213, 226, 416, 694
BsrDI GCAATG 2 cut(s) 59, 651
BsrGI TGTACA 1 cut(s) 399
BssECI CCNNGG 4 cut(s) 48, 529, 562, 607
BssMI GATC 8 cut(s) 67, 179, 218, 242, 285, 408, 443, 699
BssSI CACGAG 1 cut(s) 261
BssT1I CCWWGG 3 cut(s) 48, 529, 562
Bst2BI CACGAG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 2 cut(s) 574, 621
BstAPI GCANNNNNTGC 1 cut(s) 455
BstAUI TGTACA 1 cut(s) 399
BstC8I GCNNGC 2 cut(s) 207, 456
BstDEI CTNAG 2 cut(s) 125, 501
BstF5I GGATG 3 cut(s) 383, 439, 498
BstKTI GATC 8 cut(s) 70, 182, 221, 245, 288, 411, 446, 702
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 8 cut(s) 67, 179, 218, 242, 285, 408, 443, 699
BstMWI GCNNNNNNNGC 2 cut(s) 455, 567
BstNI CCWGG 1 cut(s) 609
BstNSI RCATGY 2 cut(s) 209, 633
BstSCI CCNGG 1 cut(s) 607
BstV1I GCAGC 1 cut(s) 31
BstX2I RGATCY 2 cut(s) 218, 443
BstXI CCANNNNNNTGG 1 cut(s) 536
BstYI RGATCY 2 cut(s) 218, 443
Bsu36I CCTNAGG 1 cut(s) 501
BsuRI GGCC 1 cut(s) 497
BtsCI GGATG 3 cut(s) 383, 439, 498
BtsIMutI CAGTG 1 cut(s) 570
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 2 cut(s) 207, 456
CciI TCATGA 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 496, 539
Csp6I GTAC 1 cut(s) 400
CviAII CATG 3 cut(s) 206, 311, 630
CviJI RGCY 7 cut(s) 90, 133, 165, 454, 497, 561, 655
CviKI_1 RGCY 7 cut(s) 90, 133, 165, 454, 497, 561, 655
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 2 cut(s) 125, 501
DpnI GATC 8 cut(s) 69, 181, 220, 244, 287, 410, 445, 701
DpnII GATC 8 cut(s) 67, 179, 218, 242, 285, 408, 443, 699
Ecl136II GAGCTC 1 cut(s) 655
Eco130I CCWWGG 3 cut(s) 48, 529, 562
Eco24I GRGCYC 1 cut(s) 657
Eco47I GGWCC 1 cut(s) 539
Eco53kI GAGCTC 1 cut(s) 655
Eco81I CCTNAGG 1 cut(s) 501
EcoICRI GAGCTC 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 3 cut(s) 48, 529, 562
EcoT38I GRGCYC 1 cut(s) 657
ErhI CCWWGG 3 cut(s) 48, 529, 562
FaeI CATG 3 cut(s) 209, 314, 633
FaqI GGGAC 2 cut(s) 347, 493
FatI CATG 3 cut(s) 205, 310, 629
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 3 cut(s) 370, 446, 505
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 1 cut(s) 45
FspBI CTAG 3 cut(s) 26, 270, 356
GluI GCNGC 1 cut(s) 45
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 1 cut(s) 497
Hin1II CATG 3 cut(s) 209, 314, 633
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 2 cut(s) 33, 247
HpaI GTTAAC 1 cut(s) 588
Hpy166II GTNNAC 3 cut(s) 328, 371, 588
Hpy188I TCNGA 1 cut(s) 240
Hpy188III TCNNGA 5 cut(s) 8, 270, 311, 490, 523
Hpy8I GTNNAC 3 cut(s) 328, 371, 588
HpyCH4III ACNGT 2 cut(s) 574, 621
HpyCH4V TGCA 9 cut(s) 209, 347, 419, 449, 458, 485, 515, 570, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 567
HpyF3I CTNAG 2 cut(s) 125, 501
Hsp92II CATG 3 cut(s) 209, 314, 633
Ksp22I TGATCA 1 cut(s) 67
KspAI GTTAAC 1 cut(s) 588
Kzo9I GATC 8 cut(s) 67, 179, 218, 242, 285, 408, 443, 699
LmnI GCTCC 5 cut(s) 87, 202, 558, 652, 660
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 1 cut(s) 424
MaeI CTAG 3 cut(s) 26, 270, 356
MalI GATC 8 cut(s) 69, 181, 220, 244, 287, 410, 445, 701
MboI GATC 8 cut(s) 67, 179, 218, 242, 285, 408, 443, 699
MboII GAAGA 1 cut(s) 299
MflI RGATCY 2 cut(s) 218, 443
MhlI GDGCHC 1 cut(s) 657
MmeI TCCRAC 1 cut(s) 558
MnlI CCTC 7 cut(s) 78, 270, 496, 552, 669, 678, 679
MroXI GAANNNNTTC 1 cut(s) 621
MseI TTAA 2 cut(s) 587, 636
MspA1I CMGCKG 1 cut(s) 548
MspR9I CCNGG 1 cut(s) 609
Mva1269I GAATGC 1 cut(s) 485
MvaI CCWGG 1 cut(s) 609
MwoI GCNNNNNNNGC 2 cut(s) 455, 567
NdeII GATC 8 cut(s) 67, 179, 218, 242, 285, 408, 443, 699
NlaIII CATG 3 cut(s) 209, 314, 633
NlaIV GGNNCC 5 cut(s) 220, 498, 560, 600, 618
NspI RCATGY 2 cut(s) 209, 633
PaeI GCATGC 1 cut(s) 209
PagI TCATGA 1 cut(s) 310
PctI GAATGC 1 cut(s) 485
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 2 cut(s) 33, 247
PflMI CCANNNNNTGG 1 cut(s) 322
PkrI GCNGC 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 657
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 5 cut(s) 220, 498, 560, 600, 618
PspPI GGNCC 2 cut(s) 496, 539
PsuI RGATCY 2 cut(s) 218, 443
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
SacI GAGCTC 1 cut(s) 657
SaqAI TTAA 2 cut(s) 587, 636
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 8 cut(s) 67, 179, 218, 242, 285, 408, 443, 699
Sau96I GGNCC 2 cut(s) 496, 539
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 1 cut(s) 657
SetI ASST 9 cut(s) 92, 214, 456, 507, 535, 544, 600, 657, 690
SfaNI GCATC 1 cut(s) 424
SinI GGWCC 1 cut(s) 539
SphI GCATGC 1 cut(s) 209
SsiI CCGC 1 cut(s) 548
SspI AATATT 2 cut(s) 362, 424
SspMI CTAG 3 cut(s) 26, 270, 356
SstI GAGCTC 1 cut(s) 657
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 3 cut(s) 48, 529, 562
TaaI ACNGT 2 cut(s) 574, 621
TaqI TCGA 3 cut(s) 171, 226, 245
TatI WGTACW 1 cut(s) 399
TfiI GAWTC 2 cut(s) 33, 247
Tru1I TTAA 2 cut(s) 587, 636
Tru9I TTAA 2 cut(s) 587, 636
TscAI CASTG 1 cut(s) 577
TseI GCWGC 1 cut(s) 44
TspDTI ATGAA 3 cut(s) 25, 48, 299
TspRI CASTG 1 cut(s) 577
Van91I CCANNNNNTGG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 539
XbaI TCTAGA 1 cut(s) 269
XceI RCATGY 2 cut(s) 209, 633
XcmI CCANNNNNNNNNTGG 1 cut(s) 552
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 3 cut(s) 26, 270, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.