Rorug01G0225500

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000243
Physical Location & Seq
Reverse (-)
32389697 .. 32396750
7054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0225500.1

Sequence Viewer

Length: 3606 bp
ATGGCTCTGTTCCGAGGTCACAAGTCCTTCCTTTGCAAATACACATCTCCTTCTCCATATAGCTATCACGTGTTCTTGAGCTTCAGAGGTGACGATACTCGAAAGAACTTCACTGATCACCTCTACAAGGCTCTGGTCGACGCCGGATTTCATACTTTCCGGGATGACCCTGAACTCGAGAGAGGGGAAAATATCAAGGAAAAGCTTGAGAACGCCATTCAACAGTCACAGAGTTCTGTGATTGTGTTTTCGAAAGACTACACCTCTTCGAGATGGTGTCTGGACGAGCTTGTCATGATCCTTGAACGCAAGAGGACCTCCGATCACGTCGTGTTACCCGTCTTCTACGACGTTAATCCATCTCAACTGAGGAAGCAGGCAGAAACTCTTTCAAAGGTTCCTAAATACCAAGAAAATCAATCGTCGGAGAAGCTGAATGGATGGCGGGCAGCGCTTGGCGAAGTTGCAGATCTAGCAGGAATGGTTTTAGAAAACGAAACCGATGGGTACGAGTCAAAGTTCATCCAAAAGATTGTTAGAGAGATCCAAGACAAGCTAGGTGGTGTGCCCCTGACCGATGTCCAGGAGGAGTTACAGAAGAAGTTGTTGTTTATTAACCCTTACCTCGATGACGCCGAGGGGAAGCAACTGAAAAACCCAACTGTGAAGGCATGGCTCAATGAGCTTAAAGAAGCTGTCTATGATACCGAGGACCTAGTGCAGGAGATCAAAACAGAAGCATTACGACGAAAGATGGAACCCGAATCTGGAAGTAGCACGAGTAAGGTACAAGACCTCAACTCTAGTCTTCCATTTCATGCTTTTGATTCAGCATCAATATACCGGAGGGCAGAGGAATTTCATAAGAAACTAGACTCCATTATGGAGCGGAGAAGAGACGTCTTCGGTATGGAAGCAACTGTTGGATACAGGGTATCACAAACAACATCGTCAACTTCTTTGCTCGAAGACTCTGGTGTGTGTGGTAGAGATGACGAGAAGGAGACACTTATTAAATTGTTGCTATCAGATGATGAGAGTGGCAATAAGATAAGTGTGATTCCTATTGTGGGTATGGGTGGGATCGGAAAGACCACCCTTGCCCGGCTTTTGTACAACGATGATGGAGTCAAACAACATTTTGACAAAAAAGCATGGGCTTCTGTTTCTGAAAAATTTGATGTTCTTCGGATCTCACAACAAATTTATGAGTCCCTCACCAAAAAAGCTTGCAATATCACGAACCTAGATCTGCTTCAATCAACGTTGCAGGAGGCTTTGATGGGGAAAAGGTTCTTCTTTGTTCTTGATGATGTGTGGAATAAGAGTTATACTGAATGGGAGAAGTTAATGAGTCCTTTTCAATTTGGAGCACCTGGAAGTCAGATTATTGTCACAACACGCAATCAAGAAGTTGCATGTATGGTGTGTGCTCTTCAAGCTCGCTGTCTCAGGCCAATGTCTGCAGAAGATAGCTGGTCATTATTTGAAAAACATGCCTTCAAAAATATAGGTGATGGTCCACGCTCACATCTTGAACAAATAGGTAAACAAATTGTACGAAAGTGCAATGGTCTTCCTTTGGCTATTAAGTCCCTTGCAGGTCTCCTATGTTCTAAATCAAATGTGGATGAATGGGAAACTATATTGAACAGTGAGATGTGGGAGTTGCCAGAGGAGGAGAGTAACATTTTGCCATCTCTTTGGTTGAGCTTTAAGCATCTGCCTCCACATCTCAGGCGTTGTTTTGTGTATTGCTCGATATTTCCCAAGAACTATAAGTTCAAATCATCAGAATTGGTTTACTTGTGGATGGCTGAAGATCTATTGCAACCCACAAGAAACAAAACAGCAGAAGAAGTCGGACAAGGCTACTTCAATGATCTAATCTCAAGATCATTTTTTCAACTTTCATCAAGTTCCGATGGATATTTCATCATGCATGACCTTATCAATGATTTAGCAAGCTTTGTATTTGGAGAATTTTGTTTTAGGTGGGACGGCAGTGATTCACCGAACAATTTGAGAAAGACTCGTCATTTTTCATATATGCTGAAAGATCATGATGAGGCTGATAGATATGTGTTTGAGGCTTTACAGCAAGCTAAATGTTTGCACACCTTTCTACCATTAGTACCATCTTCTTTGTCGCGGCCCTCATGGGGCAAAGGACTCTCTAAGGCATTGCCAAAGTTTCAATGTTTAAGAATGCTCAAGTTACCATGGTATGATATTAAGGAATTGCCTGATTCAATTAACAACTTGAAACATCTTAAGCACTTGGACTTGTCTTACAGTACCATCAAAAAGTTACCTGATACAATTTGTACTTTGTATAACTTGCAAGTATTGTTGTTGTCACAGTGTTTATGCCTTACTGAACTGCCAGCCAACTTGGGAAGATTGATCAACTTGAGCCATCTGGATATTACAGATGTATCGATAAAAAAGATGCCACCACAAATGGGTAAGTTGAAAGACCTCCAGATGCTACCAGCGTTTGTGGTAGACAAACACACTGCTGGGGATAATTTGGCAGAGTTAAAGAAGCTTGAAAAATTGCGTGGAAGACTTTGTATCTCAAAGCTTGCGCATAGTAGCAGTTTGGAGGCCTACATATTGAGGGACAAGAAGTTTCTTAAGAAACTGTATTTGGATTATTGGGGAGGATGGGATTCCAGTGAAGAAGGCAATAATACTGTAGAAGAAAGAGAAGTGCTTGAGAAGCTCCAACCTCACCTGAACCTGGAAAGGCTTACAATTAGAGGTTATGGAGACAAAATGTTTCCAGGTTGGTCAGGTTATTATTCTTCCTCTGCTCTTGTTAGTCTTGAACTTCTTTATTGTAAGAATTGTATCTCCTTGCCACCATTGGGACAGCTACCTTCCCTCAGAGCGCTTGTTATAGATGGGTTAAGGGCAGTGGTATCCATAGGTTCTGAATTCTATTATGGTGACGGCAATACTACTACTTGTGTGAATAAACCATTTAGATGTCTACGGTCTCTAATATTCGACAATATGCAGGGGTGGAAAGAATGGTGTTATGTTGGAGGTGACAATAATGAAGGTGGGGTTTTTTCTAATCTTGAGCTTCAGGGTCGAGCTGATAGTAATGGTTTTCTGTTTTCTTATGCCAGTGTATATCAGCTCTCTAGAAGTTATACTTTTGCAATTATATGCAGCGCTACCACCAAAGCCTTAGGCCCTGCATTTTGGAAAAGATGGAGAGAGAAAGGTGAACTAAGATTGTTATTTCCTGACAGTCCCAGTTGCAGTGCTGCCAATGAAGAAAATGAAGGCAGAGAGAGGAAGTCATTTTCTGATTTTGGAGAAGTCTGTTTCAGTCACAAGGAAGATAACCTAGTATGCAGTCCTCAACTACCACTTATCGTATTCTTGCCATGGTACAATTCAAGCAGAGCTCTATCTATAATAGTGCTCATCCAAAGACTTCATCATGGAAACCCGAAGGAGAAAATAGCAGCTGCTGCTCATGAGAGGGTGTCGAATGTGATGAAGAGACTGGTCATATCATTGGTCTTCATCTTAGTAGCAGTTGTCTCTATGGCTCTGCCAACTCCAGCAACCGCCTTTTCCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1201

Amino Acids

136.69

Weight (kDa)

6.83

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 193 9.7e-44 TIR domain
TIR_2 PF13676 24 - 123 7.9e-17 TIR domain
Rx_N PF18052 194 - 257 2.4e-16 Rx N-terminal domain
NB-ARC PF00931 331 - 503 2.1e-36 NB-ARC domain
WHD_DRP PF23559 588 - 655 3.3e-25 Disease resistance protein Winged helix domain
LRR_14 PF23598 727 - 1006 4e-26 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 845 - 971 4.8e-28 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 290
AatII GACGTC 1 cut(s) 903
Acc16I TGCGCA 1 cut(s) 2592
Acc36I ACCTGC 1 cut(s) 1592
AccBSI CCGCTC 1 cut(s) 889
AccI GTMKAC 3 cut(s) 138, 2508, 2998
AccII CGCG 1 cut(s) 2152
AciI CCGC 4 cut(s) 445, 889, 2152, 3591
AclI AACGTT 1 cut(s) 1265
AclWI GGATC 4 cut(s) 292, 538, 1091, 1199
AcsI RAATTY 5 cut(s) 857, 1175, 1203, 1982, 2942
AcuI CTGAAG 3 cut(s) 67, 1839, 3080
AcvI CACGTG 1 cut(s) 70
AcyI GRCGYC 3 cut(s) 141, 633, 900
AdeI CACNNNGTG 1 cut(s) 331
AfaI GTAC 8 cut(s) 509, 789, 1115, 1560, 2136, 2299, 2329, 3410
AfeI AGCGCT 3 cut(s) 453, 2898, 3187
AfiI CCNNNNNNNGG 9 cut(s) 127, 721, 767, 1070, 1104, 2162, 2465, 2746, 2873
AflII CTTAAG 2 cut(s) 2273, 2639
AflIII ACRYGT 1 cut(s) 69
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 4 cut(s) 582, 1375, 2745, 2788
AjuI GAANNNNNNNTTGG 4 cut(s) 906, 938, 2636, 2668
Alw21I GWGCWC 4 cut(s) 1375, 1435, 3427, 3444
Alw26I GTCTC 8 cut(s) 891, 998, 1454, 1610, 2769, 3009, 3517, 3568
AlwI GGATC 4 cut(s) 292, 538, 1091, 1199
AlwNI CAGNNNCTG 1 cut(s) 3491
Ama87I CYCGRG 1 cut(s) 176
Aor51HI AGCGCT 3 cut(s) 453, 2898, 3187
AoxI GGCC 4 cut(s) 1454, 2153, 2610, 3205
ApeKI GCWGC 6 cut(s) 449, 3183, 3281, 3485, 3488, 3491
ApoI RAATTY 5 cut(s) 857, 1175, 1203, 1982, 2942
Asp700I GAANNNNTTC 1 cut(s) 1292
AspLEI GCGC 4 cut(s) 454, 2593, 2899, 3188
AspS9I GGNCC 5 cut(s) 315, 712, 1520, 2154, 3206
AsuC2I CCSGG 2 cut(s) 161, 1105
AsuHPI GGTGA 9 cut(s) 101, 110, 1210, 1526, 2004, 2729, 2966, 3068, 3251
AsuII TTCGAA 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 3 cut(s) 315, 712, 1520
AxyI CCTNAGG 1 cut(s) 3202
BaeGI GKGCMC 1 cut(s) 570
BaeI ACNNNNGTAYC 2 cut(s) 2421, 2454
BanII GRGCYC 1 cut(s) 3427
BauI CACGAG 1 cut(s) 778
BbrPI CACGTG 1 cut(s) 70
BbsI GAAGAC 7 cut(s) 334, 800, 895, 975, 1568, 2575, 3535
Bbv12I GWGCWC 4 cut(s) 1375, 1435, 3427, 3444
BbvI GCAGC 6 cut(s) 461, 3195, 3268, 3475, 3478, 3497
BceAI ACGGC 2 cut(s) 2017, 2974
BciT130I CCWGG 4 cut(s) 584, 1377, 2747, 2790
BciVI GTATCC 2 cut(s) 920, 2938
BclI TGATCA 2 cut(s) 115, 2406
BcnI CCSGG 2 cut(s) 161, 1105
BcoDI GTCTC 8 cut(s) 891, 998, 1454, 1610, 2769, 3009, 3517, 3568
BfaI CTAG 9 cut(s) 473, 557, 716, 804, 872, 1247, 3156, 3365, 3604
BfmI CTRYAG 2 cut(s) 1464, 2700
BfoI RGCGCY 3 cut(s) 455, 2900, 3189
BfrI CTTAAG 2 cut(s) 2273, 2639
BfuAI ACCTGC 1 cut(s) 1592
BfuI GTATCC 2 cut(s) 920, 2938
BglII AGATCT 3 cut(s) 469, 1249, 1822
BisI GCNGC 7 cut(s) 450, 2153, 3184, 3282, 3486, 3489, 3492
BlsI GCNGC 7 cut(s) 451, 2154, 3185, 3283, 3487, 3490, 3493
Bme1390I CCNGG 6 cut(s) 161, 584, 1105, 1377, 2747, 2790
Bme18I GGWCC 3 cut(s) 315, 712, 1520
BmeT110I CYCGRG 1 cut(s) 176
BmgBI CACGTC 1 cut(s) 328
BmgT120I GGNCC 5 cut(s) 315, 712, 1520, 2154, 3206
BmiI GGNNCC 2 cut(s) 399, 759
BmrFI CCNGG 6 cut(s) 161, 584, 1105, 1377, 2747, 2790
BmrI ACTGGG 1 cut(s) 3264
BmsI GCATC 4 cut(s) 842, 1729, 2442, 2478
BmuI ACTGGG 1 cut(s) 3264
BoxI GACNNNNGTC 1 cut(s) 578
BpiI GAAGAC 7 cut(s) 334, 800, 895, 975, 1568, 2575, 3535
BplI GAGNNNNNCTC 2 cut(s) 2017, 2049
BpmI CTGGAG 2 cut(s) 2468, 3567
Bpu14I TTCGAA 1 cut(s) 251
BpuEI CTTGAG 7 cut(s) 97, 227, 1876, 2198, 2434, 2741, 3110
BpuMI CCSGG 2 cut(s) 161, 1105
Bsa29I ATCGAT 1 cut(s) 2441
BsaAI YACGTR 1 cut(s) 70
BsaHI GRCGYC 3 cut(s) 141, 633, 900
BsaI GGTCTC 2 cut(s) 1610, 3009
BsaJI CCNNGG 5 cut(s) 13, 636, 708, 2222, 3404
BsaWI WCCGGW 1 cut(s) 843
Bsc4I CCNNNNNNNGG 9 cut(s) 127, 721, 767, 1070, 1104, 2162, 2465, 2746, 2873
Bse1I ACTGG 4 cut(s) 2679, 3138, 3270, 3531
Bse21I CCTNAGG 1 cut(s) 3202
Bse3DI GCAATG 2 cut(s) 1576, 2183
BseBI CCWGG 4 cut(s) 584, 1377, 2747, 2790
BseCI ATCGAT 1 cut(s) 2441
BseDI CCNNGG 5 cut(s) 13, 636, 708, 2222, 3404
BseGI GGATG 7 cut(s) 169, 446, 522, 1636, 1818, 2675, 3444
BseLI CCNNNNNNNGG 9 cut(s) 127, 721, 767, 1070, 1104, 2162, 2465, 2746, 2873
BseMI GCAATG 2 cut(s) 1576, 2183
BseMII CTCAG 4 cut(s) 359, 1465, 1750, 2905
BseNI ACTGG 4 cut(s) 2679, 3138, 3270, 3531
BseRI GAGGAG 3 cut(s) 602, 1691, 1694
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 6 cut(s) 461, 3195, 3268, 3475, 3478, 3497
BseYI CCCAGC 1 cut(s) 2522
BsgI GTGCAG 1 cut(s) 740
Bsh1236I CGCG 1 cut(s) 2152
BshFI GGCC 4 cut(s) 1456, 2155, 2612, 3207
BshVI ATCGAT 1 cut(s) 2441
BsiHKAI GWGCWC 4 cut(s) 1375, 1435, 3427, 3444
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 4 cut(s) 144, 160, 844, 1105
BslFI GGGAC 6 cut(s) 1198, 1579, 2012, 2639, 2889, 3252
BslI CCNNNNNNNGG 9 cut(s) 127, 721, 767, 1070, 1104, 2162, 2465, 2746, 2873
BsmAI GTCTC 8 cut(s) 891, 998, 1454, 1610, 2769, 3009, 3517, 3568
BsmBI CGTCTC 1 cut(s) 891
BsmFI GGGAC 6 cut(s) 1198, 1579, 2012, 2639, 2889, 3252
BsmI GAATGC 1 cut(s) 2214
BsnI GGCC 4 cut(s) 1456, 2155, 2612, 3207
Bso31I GGTCTC 2 cut(s) 1610, 3009
BsoBI CYCGRG 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 251
Bsp1286I GDGCHC 5 cut(s) 570, 1375, 1435, 3427, 3444
Bsp1407I TGTACA 1 cut(s) 1113
Bsp19I CCATGG 2 cut(s) 2222, 3404
BspACI CCGC 4 cut(s) 445, 889, 2152, 3591
BspANI GGCC 4 cut(s) 1456, 2155, 2612, 3207
BspCNI CTCAG 4 cut(s) 360, 1464, 1749, 2904
BspDI ATCGAT 1 cut(s) 2441
BspFNI CGCG 1 cut(s) 2152
BspHI TCATGA 3 cut(s) 294, 2062, 3496
BspLI GGNNCC 2 cut(s) 399, 759
BspMAI CTGCAG 1 cut(s) 1468
BspMI ACCTGC 1 cut(s) 1592
BspPI GGATC 4 cut(s) 292, 538, 1091, 1199
BspQI GCTCTTC 1 cut(s) 1440
BspT104I TTCGAA 1 cut(s) 251
BspTI CTTAAG 2 cut(s) 2273, 2639
BspTNI GGTCTC 2 cut(s) 1610, 3009
BsrBI CCGCTC 1 cut(s) 889
BsrDI GCAATG 2 cut(s) 1576, 2183
BsrGI TGTACA 1 cut(s) 1113
BsrI ACTGG 4 cut(s) 2679, 3138, 3270, 3531
BssECI CCNNGG 5 cut(s) 13, 636, 708, 2222, 3404
BssNI GRCGYC 3 cut(s) 141, 633, 900
BssSI CACGAG 1 cut(s) 778
BssT1I CCWWGG 2 cut(s) 2222, 3404
Bst2BI CACGAG 1 cut(s) 778
Bst2UI CCWGG 4 cut(s) 584, 1377, 2747, 2790
Bst6I CTCTTC 4 cut(s) 271, 889, 1440, 3515
BstACI GRCGYC 3 cut(s) 141, 633, 900
BstAFI CTTAAG 2 cut(s) 2273, 2639
BstAPI GCANNNNNTGC 1 cut(s) 3491
BstAUI TGTACA 1 cut(s) 1113
BstBAI YACGTR 1 cut(s) 70
BstBI TTCGAA 1 cut(s) 251
BstC8I GCNNGC 8 cut(s) 378, 447, 1231, 1444, 1966, 2103, 2388, 2589
BstDEI CTNAG 8 cut(s) 368, 1451, 1736, 2178, 2891, 3202, 3245, 3550
BstDSI CCRYGG 2 cut(s) 2222, 3404
BstENI CCTNNNNNAGG 2 cut(s) 125, 719
BstF5I GGATG 7 cut(s) 169, 446, 522, 1636, 1818, 2675, 3444
BstFNI CGCG 1 cut(s) 2152
BstH2I RGCGCY 3 cut(s) 455, 2900, 3189
BstHHI GCGC 4 cut(s) 454, 2593, 2899, 3188
BstMAI GTCTC 8 cut(s) 891, 998, 1454, 1610, 2769, 3009, 3517, 3568
BstMWI GCNNNNNNNGC 6 cut(s) 451, 473, 682, 1439, 2725, 3491
BstNI CCWGG 4 cut(s) 584, 1377, 2747, 2790
BstNSI RCATGY 2 cut(s) 1422, 1499
BstPAI GACNNNNGTC 1 cut(s) 578
BstSCI CCNGG 6 cut(s) 159, 582, 1103, 1375, 2745, 2788
BstSFI CTRYAG 2 cut(s) 1464, 2700
BstSLI GKGCMC 1 cut(s) 570
BstUI CGCG 1 cut(s) 2152
BstV1I GCAGC 6 cut(s) 461, 3195, 3268, 3475, 3478, 3497
BstV2I GAAGAC 7 cut(s) 334, 800, 895, 975, 1568, 2575, 3535
BstX2I RGATCY 5 cut(s) 469, 543, 1191, 1249, 1822
BstXI CCANNNNNNTGG 1 cut(s) 1704
BstYI RGATCY 5 cut(s) 469, 543, 1191, 1249, 1822
Bsu15I ATCGAT 1 cut(s) 2441
Bsu36I CCTNAGG 1 cut(s) 3202
BsuI GTATCC 2 cut(s) 920, 2938
BsuRI GGCC 4 cut(s) 1456, 2155, 2612, 3207
BsuTUI ATCGAT 1 cut(s) 2441
BtgI CCRYGG 2 cut(s) 2222, 3404
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 7 cut(s) 169, 446, 522, 1636, 1818, 2675, 3444
BtsI GCAGTG 4 cut(s) 2011, 2517, 2928, 3283
BtsIMutI CAGTG 9 cut(s) 111, 1660, 2011, 2369, 2517, 2686, 2928, 3145, 3283
BveI ACCTGC 1 cut(s) 1592
Cac8I GCNNGC 8 cut(s) 378, 447, 1231, 1444, 1966, 2103, 2388, 2589
CaiI CAGNNNCTG 1 cut(s) 3491
CciI TCATGA 3 cut(s) 294, 2062, 3496
CfoI GCGC 4 cut(s) 454, 2593, 2899, 3188
Cfr13I GGNCC 5 cut(s) 315, 712, 1520, 2154, 3206
ClaI ATCGAT 1 cut(s) 2441
CseI GACGC 2 cut(s) 149, 641
Csp6I GTAC 8 cut(s) 508, 788, 1114, 1559, 2135, 2298, 2328, 3409
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviQI GTAC 8 cut(s) 508, 788, 1114, 1559, 2135, 2298, 2328, 3409
DdeI CTNAG 8 cut(s) 368, 1451, 1736, 2178, 2891, 3202, 3245, 3550
DraIII CACNNNGTG 1 cut(s) 331
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
Eam1104I CTCTTC 4 cut(s) 271, 889, 1440, 3515
EarI CTCTTC 4 cut(s) 271, 889, 1440, 3515
Ecl136II GAGCTC 1 cut(s) 3425
Eco130I CCWWGG 2 cut(s) 2222, 3404
Eco147I AGGCCT 1 cut(s) 2612
Eco24I GRGCYC 1 cut(s) 3427
Eco31I GGTCTC 2 cut(s) 1610, 3009
Eco47I GGWCC 3 cut(s) 315, 712, 1520
Eco47III AGCGCT 3 cut(s) 453, 2898, 3187
Eco53kI GAGCTC 1 cut(s) 3425
Eco57I CTGAAG 3 cut(s) 67, 1839, 3080
Eco72I CACGTG 1 cut(s) 70
Eco81I CCTNAGG 1 cut(s) 3202
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 3425
EcoNI CCTNNNNNAGG 2 cut(s) 125, 719
EcoO109I RGGNCCY 3 cut(s) 315, 712, 3206
EcoRI GAATTC 1 cut(s) 2942
EcoRII CCWGG 4 cut(s) 582, 1375, 2745, 2788
EcoT14I CCWWGG 2 cut(s) 2222, 3404
EcoT22I ATGCAT 1 cut(s) 1944
EcoT38I GRGCYC 1 cut(s) 3427
ErhI CCWWGG 2 cut(s) 2222, 3404
Esp3I CGTCTC 1 cut(s) 891
FalI AAGNNNNNCTT 2 cut(s) 3151, 3183
FaqI GGGAC 6 cut(s) 1198, 1579, 2012, 2639, 2889, 3252
FauI CCCGC 1 cut(s) 438
FbaI TGATCA 2 cut(s) 115, 2406
FblI GTMKAC 3 cut(s) 138, 2508, 2998
Fnu4HI GCNGC 7 cut(s) 450, 2153, 3184, 3282, 3486, 3489, 3492
FokI GGATG 7 cut(s) 176, 453, 509, 1643, 1825, 2682, 3431
FriOI GRGCYC 1 cut(s) 3427
Fsp4HI GCNGC 7 cut(s) 450, 2153, 3184, 3282, 3486, 3489, 3492
FspBI CTAG 9 cut(s) 473, 557, 716, 804, 872, 1247, 3156, 3365, 3604
FspI TGCGCA 1 cut(s) 2592
GlaI GCGC 4 cut(s) 453, 2592, 2898, 3187
GluI GCNGC 7 cut(s) 450, 2153, 3184, 3282, 3486, 3489, 3492
GsaI CCCAGC 1 cut(s) 2526
GsuI CTGGAG 2 cut(s) 2468, 3567
HaeII RGCGCY 3 cut(s) 455, 2900, 3189
HaeIII GGCC 4 cut(s) 1456, 2155, 2612, 3207
HapII CCGG 4 cut(s) 144, 160, 844, 1105
HgaI GACGC 2 cut(s) 149, 641
HhaI GCGC 4 cut(s) 454, 2593, 2899, 3188
Hin1I GRCGYC 3 cut(s) 141, 633, 900
Hin6I GCGC 4 cut(s) 452, 2591, 2897, 3186
HinP1I GCGC 4 cut(s) 452, 2591, 2897, 3186
HincII GTYRAC 2 cut(s) 139, 954
HindII GTYRAC 2 cut(s) 139, 954
HindIII AAGCTT 5 cut(s) 203, 1227, 1966, 2549, 2585
HpaII CCGG 4 cut(s) 144, 160, 844, 1105
HphI GGTGA 9 cut(s) 101, 110, 1210, 1526, 2004, 2729, 2966, 3068, 3251
Hpy166II GTNNAC 8 cut(s) 139, 954, 1523, 1550, 1804, 2509, 2999, 3242
Hpy8I GTNNAC 8 cut(s) 139, 954, 1523, 1550, 1804, 2509, 2999, 3242
Hpy99I CGWCG 5 cut(s) 143, 332, 353, 427, 750
HpyCH4IV ACGT 5 cut(s) 69, 327, 351, 900, 1265
HpyF10VI GCNNNNNNNGC 6 cut(s) 451, 473, 682, 1439, 2725, 3491
HpyF3I CTNAG 8 cut(s) 368, 1451, 1736, 2178, 2891, 3202, 3245, 3550
HpySE526I ACGT 5 cut(s) 69, 327, 351, 900, 1265
Hsp92I GRCGYC 3 cut(s) 141, 633, 900
HspAI GCGC 4 cut(s) 452, 2591, 2897, 3186
Ksp22I TGATCA 2 cut(s) 115, 2406
LguI GCTCTTC 1 cut(s) 1440
LmnI GCTCC 3 cut(s) 886, 1370, 2733
Lsp1109I GCAGC 6 cut(s) 461, 3195, 3268, 3475, 3478, 3497
LweI GCATC 4 cut(s) 842, 1729, 2442, 2478
MaeI CTAG 9 cut(s) 473, 557, 716, 804, 872, 1247, 3156, 3365, 3604
MaeII ACGT 5 cut(s) 69, 327, 351, 900, 1265
MbiI CCGCTC 1 cut(s) 889
MflI RGATCY 5 cut(s) 469, 543, 1191, 1249, 1822
MhlI GDGCHC 5 cut(s) 570, 1375, 1435, 3427, 3444
MlyI GAGTC 8 cut(s) 521, 869, 965, 1137, 1220, 1363, 2026, 2166
MmeI TCCRAC 5 cut(s) 405, 904, 1843, 2755, 3031
Mph1103I ATGCAT 1 cut(s) 1944
MroXI GAANNNNTTC 1 cut(s) 1292
MslI CAYNNNNRTG 1 cut(s) 2992
MspA1I CMGCKG 1 cut(s) 3488
MspCI CTTAAG 2 cut(s) 2273, 2639
MspI CCGG 4 cut(s) 144, 160, 844, 1105
MspR9I CCNGG 6 cut(s) 161, 584, 1105, 1377, 2747, 2790
Mva1269I GAATGC 1 cut(s) 2214
MvaI CCWGG 4 cut(s) 584, 1377, 2747, 2790
MvnI CGCG 1 cut(s) 2152
MwoI GCNNNNNNNGC 6 cut(s) 451, 473, 682, 1439, 2725, 3491
NciI CCSGG 2 cut(s) 161, 1105
NcoI CCATGG 2 cut(s) 2222, 3404
NlaIV GGNNCC 2 cut(s) 399, 759
NmeAIII GCCGAG 1 cut(s) 661
NmuCI GTSAC 8 cut(s) 17, 89, 225, 1393, 2358, 2954, 3056, 3347
NsbI TGCGCA 1 cut(s) 2592
NsiI ATGCAT 1 cut(s) 1944
NspI RCATGY 2 cut(s) 1422, 1499
NspV TTCGAA 1 cut(s) 251
PaeR7I CTCGAG 1 cut(s) 176
PagI TCATGA 3 cut(s) 294, 2062, 3496
PceI AGGCCT 1 cut(s) 2612
PciSI GCTCTTC 1 cut(s) 1440
PcsI WCGNNNNNNNCGW 2 cut(s) 336, 633
PctI GAATGC 1 cut(s) 2214
PdmI GAANNNNTTC 1 cut(s) 1292
PfeI GAWTC 6 cut(s) 764, 827, 1060, 2009, 2249, 2675
PfoI TCCNGGA 2 cut(s) 159, 582
PkrI GCNGC 7 cut(s) 451, 2154, 3185, 3283, 3487, 3490, 3493
PleI GAGTC 8 cut(s) 520, 869, 965, 1136, 1219, 1362, 2026, 2166
PmaCI CACGTG 1 cut(s) 70
PmlI CACGTG 1 cut(s) 70
PpsI GAGTC 8 cut(s) 520, 869, 965, 1136, 1219, 1362, 2026, 2166
Ppu21I YACGTR 1 cut(s) 70
PpuMI RGGWCCY 2 cut(s) 315, 712
PshAI GACNNNNGTC 1 cut(s) 578
Psp124BI GAGCTC 1 cut(s) 3427
Psp1406I AACGTT 1 cut(s) 1265
Psp5II RGGWCCY 2 cut(s) 315, 712
Psp6I CCWGG 4 cut(s) 582, 1375, 2745, 2788
PspCI CACGTG 1 cut(s) 70
PspFI CCCAGC 1 cut(s) 2522
PspGI CCWGG 4 cut(s) 582, 1375, 2745, 2788
PspN4I GGNNCC 2 cut(s) 399, 759
PspPI GGNCC 5 cut(s) 315, 712, 1520, 2154, 3206
PspPPI RGGWCCY 2 cut(s) 315, 712
PstI CTGCAG 1 cut(s) 1468
PstNI CAGNNNCTG 1 cut(s) 3491
PsuI RGATCY 5 cut(s) 469, 543, 1191, 1249, 1822
PvuII CAGCTG 1 cut(s) 3488
RsaI GTAC 8 cut(s) 509, 789, 1115, 1560, 2136, 2299, 2329, 3410
RsaNI GTAC 8 cut(s) 508, 788, 1114, 1559, 2135, 2298, 2328, 3409
RseI CAYNNNNRTG 1 cut(s) 2992
SacI GAGCTC 1 cut(s) 3427
SalI GTCGAC 1 cut(s) 137
SapI GCTCTTC 1 cut(s) 1440
SatI GCNGC 7 cut(s) 450, 2153, 3184, 3282, 3486, 3489, 3492
Sau96I GGNCC 5 cut(s) 315, 712, 1520, 2154, 3206
SchI GAGTC 8 cut(s) 521, 869, 965, 1137, 1220, 1363, 2026, 2166
ScrFI CCNGG 6 cut(s) 161, 584, 1105, 1377, 2747, 2790
SduI GDGCHC 5 cut(s) 570, 1375, 1435, 3427, 3444
SfaNI GCATC 4 cut(s) 842, 1729, 2442, 2478
SfcI CTRYAG 2 cut(s) 1464, 2700
Sfr274I CTCGAG 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 251
SinI GGWCC 3 cut(s) 315, 712, 1520
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 2992
SseBI AGGCCT 1 cut(s) 2612
SsiI CCGC 4 cut(s) 445, 889, 2152, 3591
SspI AATATT 1 cut(s) 3012
SspMI CTAG 9 cut(s) 473, 557, 716, 804, 872, 1247, 3156, 3365, 3604
SstI GAGCTC 1 cut(s) 3427
StuI AGGCCT 1 cut(s) 2612
StyD4I CCNGG 6 cut(s) 159, 582, 1103, 1375, 2745, 2788
StyI CCWWGG 2 cut(s) 2222, 3404
TaiI ACGT 5 cut(s) 72, 330, 354, 903, 1268
TaqII GACCGA 1 cut(s) 590
TatI WGTACW 2 cut(s) 1113, 2327
TauI GCSGC 1 cut(s) 2155
TfiI GAWTC 6 cut(s) 764, 827, 1060, 2009, 2249, 2675
TscAI CASTG 9 cut(s) 118, 1660, 2011, 2369, 2524, 2686, 2928, 3145, 3283
TseFI GTSAC 8 cut(s) 17, 89, 225, 1393, 2358, 2954, 3056, 3347
TseI GCWGC 6 cut(s) 449, 3183, 3281, 3485, 3488, 3491
Tsp45I GTSAC 8 cut(s) 17, 89, 225, 1393, 2358, 2954, 3056, 3347
TspGWI ACGGA 1 cut(s) 3589
TspRI CASTG 9 cut(s) 118, 1660, 2011, 2369, 2524, 2686, 2928, 3145, 3283
Vha464I CTTAAG 2 cut(s) 2273, 2639
VpaK11BI GGWCC 3 cut(s) 315, 712, 1520
XagI CCTNNNNNAGG 2 cut(s) 125, 719
XapI RAATTY 5 cut(s) 857, 1175, 1203, 1982, 2942
XbaI TCTAGA 1 cut(s) 3155
XceI RCATGY 2 cut(s) 1422, 1499
XhoI CTCGAG 1 cut(s) 176
XmiI GTMKAC 3 cut(s) 138, 2508, 2998
XmnI GAANNNNTTC 1 cut(s) 1292
XspI CTAG 9 cut(s) 473, 557, 716, 804, 872, 1247, 3156, 3365, 3604
ZraI GACGTC 1 cut(s) 901
Zsp2I ATGCAT 1 cut(s) 1944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.