RchiOBHm_Chr7g0240941

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000243
Physical Location & Seq
Forward (+)
66907812 .. 66913922
6111 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21594

Sequence Viewer

Length: 1752 bp
ATGCATCGCTTACCGCGTTTTTTCTCTAGCAACAAGGATCGAACTAAATCCATGGATCCGAAGAAGGTCCCGCGGTTGCCCAAGTTGGAGCGCCGCAATGCGGTCAAGCACATTGACTACGACGCCTCTTCGTCCTCCTCCTCCCGCGACAACTCCACCCACGCGCAGCAGCTCCACACGCGCTCGCTCGACCTGTCGGACCGGACCAGCTTCCGGGTCGAAGGAAATGACGGCGAGTTCGAAAGGATTTGCGCCGAGCTGGGCTTCTCCGGCCCAGACGACTTCGCAATACCCGAGGCGGCCTGGGAGTCCCGGAAGATCCGGTCCACCAACTCGGATGTTCTCCCCCTGTCAAAACTGTACCCGATGGATAGTCCGAGACCCGACCCGAAGGACGAATCGGAGCATGAGGATGATGCCGTGAAGGAATTGCTCAATAGAGTTAGGGATAGCGTTATTGTTACGGTGCCCGAGTCGACTCGGACGGATTCCGAGTCGGCGGGGCCGAGCGGGTGTTGTACTGCGAGTACTAGTAGTTGTAGTGTTAGTGTAGGAATTAAGGGAGCGCGGCCGCCGGTGCTGAGGCCGCCGCCGTCGATGGCTAGGGTTCCGGTGATTGACAATGGATGCTCGACTTGGGACCTTTTGAGGGACTTCGCTCCAGAAGAAGAGAGGGATATGGTGCGGAGCAGGTTTGTTCCTTCTTCTTCTGATGAAGAAGATGAAGAAGAAGAACTAGAAGAAGAACTTGAAGGAGAGGATGTTGTGGCGGATGAGATTAGAGAAACCGTGGCAAACTCCGGCGGGTGTTCGTTTACTACTTCCAATGACGACGACTCTTCCAGCACTACCACAGATCCTTCCAATATTTCCCCAAATGGAAGGTTTTCGCCGAATGGGAAGCCTAAGCTTATTGTCACTAGTTGGGAGAAGGGTGATCTTCTCGGCAGCGGCTCTTTCGGGTCTGTGTATGAAGGAATTTCTGATGGCGGATGCTTCATTGCTGTTAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGAAGGCAAAGAGTTTCTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGAATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAGACGTACCATCTTACAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCGAAGAGTGATTCACAGGGACGTTAAATGTGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCAGACTTTGGATTGGCAAAGACTATCAAAATGAACGACATAAAATCTTGCCAAGGAACTGCATACTGGATGGCACCTGAGGTTGTTAATCGTAAGAGTCAAGGTTATGGCCTTCCTGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACTCTAATCTCGAATGGATGCAGGCATTATGGAAAATTGGAAAGGGGGAGCCCCCTCTGGTTCCTGATTCTCTTTCAAAAGAGGCACAAGATTTCATCCGTCTATGCTTACAAGTTAAGCCAGATAATCGTCCCACTGCTGCTCAGCTTTTAAAACATCCATTTGTAAATAAGCCCCTTCTTCCCACGTCTTCTGGATCAGTATCTCCTCACAATCACCACAGGCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

583

Amino Acids

64.75

Weight (kDa)

5.4

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 310 - 562 5.9e-69 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 312 - 558 7.9e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 681
AccB1I GGYRCC 2 cut(s) 466, 1417
AccBSI CCGCTC 1 cut(s) 510
AccI GTMKAC 2 cut(s) 476, 1233
AccII CGCG 6 cut(s) 16, 73, 147, 164, 181, 568
AclWI GGATC 7 cut(s) 45, 50, 63, 313, 851, 1339, 1723
AcoI YGGCCR 1 cut(s) 569
AcsI RAATTY 1 cut(s) 978
AcuI CTGAAG 1 cut(s) 1280
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 5 cut(s) 362, 520, 529, 1205, 1486
AfiI CCNNNNNNNGG 3 cut(s) 100, 213, 649
AgsI TTSAA 5 cut(s) 752, 1067, 1097, 1187, 1596
AhlI ACTAGT 2 cut(s) 530, 920
AjiI CACGTC 1 cut(s) 1707
AjnI CCWGG 2 cut(s) 302, 1038
AluBI AGCT 8 cut(s) 172, 210, 259, 910, 1147, 1192, 1342, 1666
AluI AGCT 8 cut(s) 172, 210, 259, 910, 1147, 1192, 1342, 1666
Alw26I GTCTC 2 cut(s) 373, 1231
AlwI GGATC 7 cut(s) 45, 50, 63, 313, 851, 1339, 1723
Ama87I CYCGRG 2 cut(s) 293, 470
AoxI GGCC 6 cut(s) 271, 300, 503, 569, 584, 1453
ApeKI GCWGC 4 cut(s) 166, 169, 948, 1658
ApoI RAATTY 1 cut(s) 978
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
Asp700I GAANNNNTTC 1 cut(s) 886
AspLEI GCGC 5 cut(s) 93, 166, 183, 254, 568
AspS9I GGNCC 7 cut(s) 67, 199, 204, 272, 324, 503, 640
AsuC2I CCSGG 2 cut(s) 215, 313
AsuHPI GGTGA 3 cut(s) 625, 947, 1727
AsuII TTCGAA 1 cut(s) 240
AvaI CYCGRG 2 cut(s) 293, 470
AvaII GGWCC 5 cut(s) 67, 199, 204, 324, 640
AxyI CCTNAGG 1 cut(s) 1422
BaeGI GKGCMC 1 cut(s) 471
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 2 cut(s) 466, 1417
BanII GRGCYC 1 cut(s) 1572
BbsI GAAGAC 1 cut(s) 1701
BbvCI CCTCAGC 1 cut(s) 581
BbvI GCAGC 4 cut(s) 178, 181, 960, 1645
BccI CCATC 6 cut(s) 361, 592, 980, 1215, 1408, 1504
BceAI ACGGC 3 cut(s) 247, 404, 577
BciT130I CCWGG 2 cut(s) 304, 1040
BclI TGATCA 1 cut(s) 1027
BcnI CCSGG 2 cut(s) 215, 313
BcoDI GTCTC 2 cut(s) 373, 1231
BcuI ACTAGT 2 cut(s) 530, 920
BfaI CTAG 6 cut(s) 27, 531, 603, 737, 921, 1239
BfoI RGCGCY 1 cut(s) 94
BfuAI ACCTGC 1 cut(s) 681
BglII AGATCT 1 cut(s) 1245
BlpI GCTNAGC 1 cut(s) 1662
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 4 cut(s) 215, 304, 313, 1040
Bme18I GGWCC 5 cut(s) 67, 199, 204, 324, 640
BmeT110I CYCGRG 2 cut(s) 293, 470
BmgBI CACGTC 1 cut(s) 1707
BmgT120I GGNCC 7 cut(s) 67, 199, 204, 272, 324, 503, 640
BmrFI CCNGG 4 cut(s) 215, 304, 313, 1040
BmrI ACTGGG 1 cut(s) 1515
BmsI GCATC 5 cut(s) 13, 406, 617, 983, 1527
BmuI ACTGGG 1 cut(s) 1515
BpiI GAAGAC 1 cut(s) 1701
BpmI CTGGAG 1 cut(s) 645
Bpu10I CCTNAGC 2 cut(s) 581, 906
Bpu1102I GCTNAGC 1 cut(s) 1662
Bpu14I TTCGAA 1 cut(s) 240
BpuMI CCSGG 2 cut(s) 215, 313
BsaBI GATNNNNATC 1 cut(s) 1720
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 7 cut(s) 51, 71, 294, 303, 789, 1039, 1396
BsaWI WCCGGW 3 cut(s) 201, 321, 610
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 213, 649
Bse118I RCCGGY 1 cut(s) 574
Bse1I ACTGG 2 cut(s) 1415, 1510
Bse21I CCTNAGG 1 cut(s) 1422
Bse3DI GCAATG 2 cut(s) 103, 999
Bse8I GATNNNNATC 1 cut(s) 1720
BseBI CCWGG 2 cut(s) 304, 1040
BseDI CCNNGG 7 cut(s) 51, 71, 294, 303, 789, 1039, 1396
BseJI GATNNNNATC 1 cut(s) 1720
BseLI CCNNNNNNNGG 3 cut(s) 100, 213, 649
BseMI GCAATG 2 cut(s) 103, 999
BseMII CTCAG 4 cut(s) 572, 1076, 1413, 1676
BseNI ACTGG 2 cut(s) 1415, 1510
BseRI GAGGAG 3 cut(s) 127, 130, 1716
BseSI GKGCMC 1 cut(s) 471
BseX3I CGGCCG 1 cut(s) 569
BseXI GCAGC 4 cut(s) 178, 181, 960, 1645
BseYI CCCAGC 1 cut(s) 259
Bsh1236I CGCG 6 cut(s) 16, 73, 147, 164, 181, 568
Bsh1285I CGRYCG 2 cut(s) 572, 1276
BshFI GGCC 6 cut(s) 273, 302, 505, 571, 586, 1455
BshNI GGYRCC 2 cut(s) 466, 1417
BsiEI CGRYCG 2 cut(s) 572, 1276
BsiHKCI CYCGRG 2 cut(s) 293, 470
BsiSI CCGG 8 cut(s) 202, 214, 270, 313, 322, 575, 611, 801
BslFI GGGAC 6 cut(s) 53, 295, 653, 665, 1307, 1635
BslI CCNNNNNNNGG 3 cut(s) 100, 213, 649
BsmAI GTCTC 2 cut(s) 373, 1231
BsmFI GGGAC 6 cut(s) 53, 295, 653, 665, 1307, 1635
BsnI GGCC 6 cut(s) 273, 302, 505, 571, 586, 1455
Bso31I GGTCTC 1 cut(s) 373
BsoBI CYCGRG 2 cut(s) 293, 470
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 2 cut(s) 471, 1572
Bsp143I GATC 9 cut(s) 37, 55, 318, 856, 937, 1027, 1245, 1331, 1715
Bsp1720I GCTNAGC 1 cut(s) 1662
Bsp19I CCATGG 1 cut(s) 51
BspANI GGCC 6 cut(s) 273, 302, 505, 571, 586, 1455
BspCNI CTCAG 4 cut(s) 573, 1077, 1414, 1675
BspFNI CGCG 6 cut(s) 16, 73, 147, 164, 181, 568
BspMI ACCTGC 1 cut(s) 681
BspPI GGATC 7 cut(s) 45, 50, 63, 313, 851, 1339, 1723
BspQI GCTCTTC 1 cut(s) 1086
BspT104I TTCGAA 1 cut(s) 240
BspT107I GGYRCC 2 cut(s) 466, 1417
BspTNI GGTCTC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 510
BsrDI GCAATG 2 cut(s) 103, 999
BsrFI RCCGGY 1 cut(s) 574
BsrI ACTGG 2 cut(s) 1415, 1510
BssAI RCCGGY 1 cut(s) 574
BssECI CCNNGG 7 cut(s) 51, 71, 294, 303, 789, 1039, 1396
BssMI GATC 9 cut(s) 37, 55, 318, 856, 937, 1027, 1245, 1331, 1715
BssNAI GTATAC 1 cut(s) 1234
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 2 cut(s) 51, 1396
Bst1107I GTATAC 1 cut(s) 1234
Bst2UI CCWGG 2 cut(s) 304, 1040
Bst4CI ACNGT 4 cut(s) 360, 466, 790, 1489
Bst6I CTCTTC 5 cut(s) 133, 663, 844, 1086, 1273
BstACI GRCGYC 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 4 cut(s) 185, 1323, 1344, 1542
BstDEI CTNAG 5 cut(s) 581, 906, 1085, 1422, 1662
BstDSI CCRYGG 3 cut(s) 51, 71, 789
BstFNI CGCG 6 cut(s) 16, 73, 147, 164, 181, 568
BstH2I RGCGCY 1 cut(s) 94
BstHHI GCGC 5 cut(s) 93, 166, 183, 254, 568
BstKTI GATC 9 cut(s) 40, 58, 321, 859, 940, 1030, 1248, 1334, 1718
BstMAI GTCTC 2 cut(s) 373, 1231
BstMBI GATC 9 cut(s) 37, 55, 318, 856, 937, 1027, 1245, 1331, 1715
BstMCI CGRYCG 2 cut(s) 572, 1276
BstMWI GCNNNNNNNGC 4 cut(s) 178, 270, 577, 586
BstNI CCWGG 2 cut(s) 304, 1040
BstNSI RCATGY 2 cut(s) 1226, 1325
BstSCI CCNGG 4 cut(s) 213, 302, 311, 1038
BstSLI GKGCMC 1 cut(s) 471
BstUI CGCG 6 cut(s) 16, 73, 147, 164, 181, 568
BstV1I GCAGC 4 cut(s) 178, 181, 960, 1645
BstV2I GAAGAC 1 cut(s) 1701
BstX2I RGATCY 5 cut(s) 55, 318, 856, 1245, 1331
BstYI RGATCY 5 cut(s) 55, 318, 856, 1245, 1331
BstZ17I GTATAC 1 cut(s) 1234
BstZI CGGCCG 1 cut(s) 569
Bsu36I CCTNAGG 1 cut(s) 1422
BsuRI GGCC 6 cut(s) 273, 302, 505, 571, 586, 1455
BtgI CCRYGG 3 cut(s) 51, 71, 789
BtrI CACGTC 1 cut(s) 1707
BtsI GCAGTG 1 cut(s) 1653
BtsIMutI CAGTG 1 cut(s) 1653
BveI ACCTGC 1 cut(s) 681
Cac8I GCNNGC 4 cut(s) 185, 1323, 1344, 1542
CciNI GCGGCCGC 1 cut(s) 569
CfoI GCGC 5 cut(s) 93, 166, 183, 254, 568
Cfr10I RCCGGY 1 cut(s) 574
Cfr13I GGNCC 7 cut(s) 67, 199, 204, 272, 324, 503, 640
Cfr42I CCGCGG 1 cut(s) 74
CpoI CGGWCCG 1 cut(s) 199
CseI GACGC 1 cut(s) 131
Csp6I GTAC 5 cut(s) 361, 519, 528, 1204, 1485
CspI CGGWCCG 1 cut(s) 199
CviAII CATG 5 cut(s) 52, 407, 1100, 1223, 1322
CviQI GTAC 5 cut(s) 361, 519, 528, 1204, 1485
DdeI CTNAG 5 cut(s) 581, 906, 1085, 1422, 1662
DpnI GATC 9 cut(s) 39, 57, 320, 858, 939, 1029, 1247, 1333, 1717
DpnII GATC 9 cut(s) 37, 55, 318, 856, 937, 1027, 1245, 1331, 1715
DraI TTTAAA 1 cut(s) 1671
EaeI YGGCCR 1 cut(s) 569
EagI CGGCCG 1 cut(s) 569
Eam1104I CTCTTC 5 cut(s) 133, 663, 844, 1086, 1273
EarI CTCTTC 5 cut(s) 133, 663, 844, 1086, 1273
EciI GGCGGA 2 cut(s) 785, 1005
EclXI CGGCCG 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 51, 1396
Eco24I GRGCYC 1 cut(s) 1572
Eco31I GGTCTC 1 cut(s) 373
Eco47I GGWCC 5 cut(s) 67, 199, 204, 324, 640
Eco52I CGGCCG 1 cut(s) 569
Eco57I CTGAAG 1 cut(s) 1280
Eco81I CCTNAGG 1 cut(s) 1422
Eco88I CYCGRG 2 cut(s) 293, 470
EcoO109I RGGNCCY 2 cut(s) 67, 640
EcoRII CCWGG 2 cut(s) 302, 1038
EcoT14I CCWWGG 2 cut(s) 51, 1396
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1572
ErhI CCWWGG 2 cut(s) 51, 1396
FaeI CATG 5 cut(s) 55, 410, 1103, 1226, 1325
FalI AAGNNNNNCTT 4 cut(s) 732, 764, 1167, 1199
FaqI GGGAC 6 cut(s) 53, 295, 653, 665, 1307, 1635
FatI CATG 5 cut(s) 51, 406, 1099, 1222, 1321
FauI CCCGC 5 cut(s) 78, 152, 493, 503, 797
FbaI TGATCA 1 cut(s) 1027
FblI GTMKAC 2 cut(s) 476, 1233
FriOI GRGCYC 1 cut(s) 1572
FspBI CTAG 6 cut(s) 27, 531, 603, 737, 921, 1239
GlaI GCGC 5 cut(s) 92, 165, 182, 253, 567
GsaI CCCAGC 1 cut(s) 263
GsuI CTGGAG 1 cut(s) 645
HaeII RGCGCY 1 cut(s) 94
HaeIII GGCC 6 cut(s) 273, 302, 505, 571, 586, 1455
HapII CCGG 8 cut(s) 202, 214, 270, 313, 322, 575, 611, 801
HgaI GACGC 1 cut(s) 131
HhaI GCGC 5 cut(s) 93, 166, 183, 254, 568
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 5 cut(s) 55, 410, 1103, 1226, 1325
Hin6I GCGC 5 cut(s) 91, 164, 181, 252, 566
HinP1I GCGC 5 cut(s) 91, 164, 181, 252, 566
HincII GTYRAC 2 cut(s) 477, 1503
HindII GTYRAC 2 cut(s) 477, 1503
HindIII AAGCTT 2 cut(s) 908, 1340
HpaI GTTAAC 1 cut(s) 1503
HpaII CCGG 8 cut(s) 202, 214, 270, 313, 322, 575, 611, 801
HphI GGTGA 3 cut(s) 625, 947, 1727
Hpy166II GTNNAC 5 cut(s) 327, 477, 816, 1234, 1503
Hpy188III TCNNGA 6 cut(s) 662, 1160, 1271, 1529, 1583, 1713
Hpy8I GTNNAC 5 cut(s) 327, 477, 816, 1234, 1503
Hpy99I CGWCG 3 cut(s) 125, 598, 836
HpyCH4III ACNGT 4 cut(s) 360, 466, 790, 1489
HpyCH4IV ACGT 3 cut(s) 1202, 1296, 1706
HpyCH4V TGCA 7 cut(s) 4, 1252, 1307, 1321, 1346, 1406, 1540
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 270, 577, 586
HpyF3I CTNAG 5 cut(s) 581, 906, 1085, 1422, 1662
HpySE526I ACGT 3 cut(s) 1202, 1296, 1706
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 5 cut(s) 55, 410, 1103, 1226, 1325
HspAI GCGC 5 cut(s) 91, 164, 181, 252, 566
Ksp22I TGATCA 1 cut(s) 1027
KspAI GTTAAC 1 cut(s) 1503
KspI CCGCGG 1 cut(s) 74
Kzo9I GATC 9 cut(s) 37, 55, 318, 856, 937, 1027, 1245, 1331, 1715
LguI GCTCTTC 1 cut(s) 1086
LmnI GCTCC 8 cut(s) 88, 177, 403, 563, 664, 687, 1184, 1567
Lsp1109I GCAGC 4 cut(s) 178, 181, 960, 1645
LweI GCATC 5 cut(s) 13, 406, 617, 983, 1527
MaeI CTAG 6 cut(s) 27, 531, 603, 737, 921, 1239
MaeII ACGT 3 cut(s) 1202, 1296, 1706
MaeIII GTNAC 3 cut(s) 460, 916, 1168
MalI GATC 9 cut(s) 39, 57, 320, 858, 939, 1029, 1247, 1333, 1717
MbiI CCGCTC 1 cut(s) 510
MboI GATC 9 cut(s) 37, 55, 318, 856, 937, 1027, 1245, 1331, 1715
MflI RGATCY 5 cut(s) 55, 318, 856, 1245, 1331
MhlI GDGCHC 2 cut(s) 471, 1572
MluCI AATT 4 cut(s) 428, 555, 978, 1554
MlyI GAGTC 8 cut(s) 317, 472, 482, 503, 830, 1096, 1450, 1483
MmeI TCCRAC 3 cut(s) 66, 177, 1473
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 886
MseI TTAA 7 cut(s) 558, 1008, 1299, 1431, 1502, 1635, 1670
MslI CAYNNNNRTG 2 cut(s) 411, 1326
MspA1I CMGCKG 2 cut(s) 73, 951
MspI CCGG 8 cut(s) 202, 214, 270, 313, 322, 575, 611, 801
MspR9I CCNGG 4 cut(s) 215, 304, 313, 1040
MvaI CCWGG 2 cut(s) 304, 1040
MvnI CGCG 6 cut(s) 16, 73, 147, 164, 181, 568
MwoI GCNNNNNNNGC 4 cut(s) 178, 270, 577, 586
NciI CCSGG 2 cut(s) 215, 313
NcoI CCATGG 1 cut(s) 51
NdeII GATC 9 cut(s) 37, 55, 318, 856, 937, 1027, 1245, 1331, 1715
NlaIII CATG 5 cut(s) 55, 410, 1103, 1226, 1325
NmeAIII GCCGAG 3 cut(s) 280, 531, 924
NmuCI GTSAC 1 cut(s) 916
NotI GCGGCCGC 1 cut(s) 569
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 1226, 1325
NspV TTCGAA 1 cut(s) 240
PaeI GCATGC 1 cut(s) 1325
PciSI GCTCTTC 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 4 cut(s) 13, 237, 291, 503
PdmI GAANNNNTTC 1 cut(s) 886
PfeI GAWTC 6 cut(s) 398, 488, 1139, 1217, 1285, 1586
PfoI TCCNGGA 1 cut(s) 311
PleI GAGTC 8 cut(s) 316, 472, 481, 502, 830, 1095, 1449, 1482
PpsI GAGTC 8 cut(s) 316, 472, 481, 502, 830, 1095, 1449, 1482
PpuMI RGGWCCY 2 cut(s) 67, 640
Psp5II RGGWCCY 2 cut(s) 67, 640
Psp6I CCWGG 2 cut(s) 302, 1038
PspFI CCCAGC 1 cut(s) 259
PspGI CCWGG 2 cut(s) 302, 1038
PspPI GGNCC 7 cut(s) 67, 199, 204, 272, 324, 503, 640
PspPPI RGGWCCY 2 cut(s) 67, 640
PsuI RGATCY 5 cut(s) 55, 318, 856, 1245, 1331
RsaI GTAC 5 cut(s) 362, 520, 529, 1205, 1486
RsaNI GTAC 5 cut(s) 361, 519, 528, 1204, 1485
RseI CAYNNNNRTG 2 cut(s) 411, 1326
Rsr2I CGGWCCG 1 cut(s) 199
RsrII CGGWCCG 1 cut(s) 199
SacII CCGCGG 1 cut(s) 74
SalI GTCGAC 1 cut(s) 475
SapI GCTCTTC 1 cut(s) 1086
SaqAI TTAA 7 cut(s) 558, 1008, 1299, 1431, 1502, 1635, 1670
Sau3AI GATC 9 cut(s) 37, 55, 318, 856, 937, 1027, 1245, 1331, 1715
Sau96I GGNCC 7 cut(s) 67, 199, 204, 272, 324, 503, 640
ScaI AGTACT 1 cut(s) 529
SchI GAGTC 8 cut(s) 317, 472, 482, 503, 830, 1096, 1450, 1483
ScrFI CCNGG 4 cut(s) 215, 304, 313, 1040
SduI GDGCHC 2 cut(s) 471, 1572
SfaNI GCATC 5 cut(s) 13, 406, 617, 983, 1527
Sfr303I CCGCGG 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 240
SgrAI CRCCGGYG 1 cut(s) 574
SgrBI CCGCGG 1 cut(s) 74
SinI GGWCC 5 cut(s) 67, 199, 204, 324, 640
SmiMI CAYNNNNRTG 2 cut(s) 411, 1326
SpeI ACTAGT 2 cut(s) 530, 920
SphI GCATGC 1 cut(s) 1325
Sse9I AATT 4 cut(s) 428, 555, 978, 1554
SspI AATATT 1 cut(s) 868
SspMI CTAG 6 cut(s) 27, 531, 603, 737, 921, 1239
StyD4I CCNGG 4 cut(s) 213, 302, 311, 1038
StyI CCWWGG 2 cut(s) 51, 1396
TaaI ACNGT 4 cut(s) 360, 466, 790, 1489
TaiI ACGT 3 cut(s) 1205, 1299, 1709
TaqI TCGA 8 cut(s) 40, 189, 219, 240, 476, 596, 632, 1530
TaqII GACCGA 1 cut(s) 1290
TasI AATT 4 cut(s) 428, 555, 978, 1554
TatI WGTACW 3 cut(s) 518, 527, 1484
TauI GCSGC 7 cut(s) 96, 302, 571, 574, 589, 592, 954
TfiI GAWTC 6 cut(s) 398, 488, 1139, 1217, 1285, 1586
Tru1I TTAA 7 cut(s) 558, 1008, 1299, 1431, 1502, 1635, 1670
Tru9I TTAA 7 cut(s) 558, 1008, 1299, 1431, 1502, 1635, 1670
TscAI CASTG 1 cut(s) 1660
TseFI GTSAC 1 cut(s) 916
TseI GCWGC 4 cut(s) 166, 169, 948, 1658
Tsp45I GTSAC 1 cut(s) 916
TspDTI ATGAA 7 cut(s) 729, 738, 987, 988, 1152, 1391, 1603
TspGWI ACGGA 2 cut(s) 500, 1607
TspRI CASTG 1 cut(s) 1660
VpaK11BI GGWCC 5 cut(s) 67, 199, 204, 324, 640
XapI RAATTY 1 cut(s) 978
XceI RCATGY 2 cut(s) 1226, 1325
XmiI GTMKAC 2 cut(s) 476, 1233
XmnI GAANNNNTTC 1 cut(s) 886
XspI CTAG 6 cut(s) 27, 531, 603, 737, 921, 1239
ZrmI AGTACT 1 cut(s) 529
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.