Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000243

Physical Location & Seq

Genomic Coordinates Reverse (-)

52058259 .. 52062558

Gene Structure 4300 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1419300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGGACGTCGCCAAGCAATTTAAACGTCCGAAACCCAAACTCGACCGCCAAAACGCCATTAAGAACGTCGACTACGACGCCTCTACCTCCTCCCCTTCCTCATCCTCCTCGCGCGGCCGTCACTCCCCTCTCTATGACCACCAAACCAGTTTCCGCGTCAGAGGCATTGACGGCGAATTCGACCTCATCTGCCGCAGCCTCGGGCTTTCCGGCCCCGAAGACTTCGCCATCCCAATCGCCGCTTGGGAAGCCCAGCGGGCCTGCATTTCCTCCCACGTCACCCCTCGCTCTCGCCTCCACGATCAGAATCATCATCCTCAGTTGCGCGACAGAGCAAACGTTGACGTTTTGGAGAAGAAGTCCGAAGCCAGGGTTATGCAGCTCGACAGTGAAATCAAGGCTTCGAGTTTGGCAGAATTTGGGGGAAATGGAAGAAACGGCGGTGCTGGTGGAGGAATTAAAGGTCTCCGCCCGCCTCCGGTTGTATTGAAATCTTTTTCCCCTCAGGAGGACCACATTGGTGTAAATTCGCTGAATCGCGTTGGCAATGTGAGCTATTCGGATGAAGAGGAGCTTGCAAATGAAGCAAATAGCGAAAGGGAATCGGTAAACGAAGCAAATGACGAGTGTTTGTCATCCAACTCATCTCCATCCAATGACAATAATGATGATCAAAATGCAGATGACAAAGCTGGTTTTGTTGGCATTGTAAGAATGGACCCGGAGCCAATCCATAGTATTTTTTCACCGAATGGGAAATTCAGGCGGAGTCTTTCGTCATCGTGGCAAAAAGGTCAGCTTTTGGGAAGTGGTTCTTATGGCACTGTGTATGAAGGCTTCACTGACGATGGATTCTTTTTTGCTGTAAAGGAGGTTTCTCTACTGGATCAAGGGAGCCAGGGGAAGCAGTGCGTTATCCAACTTGAACAGGAGATTTCTCTTTTAAGTCAGTTTGAACATGACAACATAGTTCAATATCTGGGCACAGACAAGGATGAAACTAAGCTCTATATCTTCCTCGAGCTTGTAACAAAAGGCTCACTTGCAAATCTATATCAGAAGTATCACTTGAGGGATTCTCAAGTCTCTGGGTTTACAAGGCAGATTTTAAGCGGGTTGACATATCTTCATGATCGTAATGTGGTTCACAGGGATATCAAATGTGCTAATATATTGGTGGATGCAAGTGGATCTGTGAAACTTGCCGACTTTGGGTTAGCCAAGGCAACCAAATTTAATGACGTGAAGTCTTGCAAAGGGACTGCTTATTGGATGGCGCCTGAGGTTGTTAATCTAAGGAATCGTGGCTATGGGCTTGCAGCTGACATATGGAGCCTTGGATGCGCTGTGTTAGAGATGTTAACCCGCCGGCCTCCATACTCTCACCTGGAAGGCATGCAAGCATTATTTCAGATTGGCAAGGGTGAACCTCCTCCAGTTCCTGATTCGTTATCAAGCGATGCACAAGATTTCATTTTGAAATGTTTACAAGTTAACCCTAATAGTCGACCTACTGCGGCTCAGCTACTGAACCATCCATTTGTGAAAAGGCCACTCCACGCATCCTCTGTCCCAGCTTCTCCTCACTGCAACAGTTTTCATTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000165 GO:0000186 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:1902531 GO:1902533

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

Pkinase

Protein Analysis

536

Amino Acids

58.86

Weight (kDa)

6.2

Isoelectric Point (pI)

45.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	262 - 515	4e-70	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	265 - 511	7.5e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca	FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica	MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica	Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis	pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis	RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata	RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora	Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii	Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa	Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha	Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana	Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	9
AccB1I	GGYRCC	1 cut(s)	1276
AccI	GTMKAC	2 cut(s)	69, 1507
AccII	CGCG	5 cut(s)	112, 114, 156, 327, 540
AclI	AACGTT	1 cut(s)	339
AclWI	GGATC	2 cut(s)	894, 1198
AcoI	YGGCCR	1 cut(s)	115
AcsI	RAATTY	5 cut(s)	176, 416, 526, 758, 1232
AcyI	GRCGYC	3 cut(s)	6, 78, 1277
AfiI	CCNNNNNNNGG	4 cut(s)	369, 478, 508, 1212
AgsI	TTSAA	5 cut(s)	490, 926, 956, 974, 1480
AhdI	GACNNNNNGTC	1 cut(s)	1246
AjiI	CACGTC	2 cut(s)	277, 1243
AjnI	CCWGG	3 cut(s)	368, 897, 1386
AleI	CACNNNNGTG	1 cut(s)	519
Alw26I	GTCTC	2 cut(s)	470, 1090
AlwI	GGATC	2 cut(s)	894, 1198
AlwNI	CAGNNNCTG	2 cut(s)	1442, 1528
Ama87I	CYCGRG	2 cut(s)	200, 1019
AoxI	GGCC	5 cut(s)	115, 211, 258, 1370, 1550
ApeKI	GCWGC	3 cut(s)	195, 379, 1319
ApoI	RAATTY	5 cut(s)	176, 416, 526, 758, 1232
ArsI	GACNNNNNNTTYG	2 cut(s)	161, 193
Asp700I	GAANNNNTTC	1 cut(s)	811
AspLEI	GCGC	4 cut(s)	114, 327, 1279, 1346
AspS9I	GGNCC	4 cut(s)	212, 258, 511, 718
AsuC2I	CCSGG	1 cut(s)	722
AsuHPI	GGTGA	4 cut(s)	271, 738, 1376, 1436
AvaI	CYCGRG	2 cut(s)	200, 1019
AvaII	GGWCC	2 cut(s)	511, 718
AxyI	CCTNAGG	2 cut(s)	504, 1281
BaeGI	GKGCMC	1 cut(s)	986
BanI	GGYRCC	1 cut(s)	1276
BarI	GAAGNNNNNNTAC	2 cut(s)	821, 853
BbsI	GAAGAC	1 cut(s)	225
BbvI	GCAGC	3 cut(s)	207, 391, 1331
BccI	CCATC	5 cut(s)	236, 658, 842, 1267, 1542
BceAI	ACGGC	3 cut(s)	102, 187, 454
BciT130I	CCWGG	3 cut(s)	370, 899, 1388
BclI	TGATCA	1 cut(s)	670
BcnI	CCSGG	1 cut(s)	722
BcoDI	GTCTC	2 cut(s)	470, 1090
BfoI	RGCGCY	1 cut(s)	1280
BglI	GCCNNNNNGGC	1 cut(s)	257
BisI	GCNGC	7 cut(s)	115, 193, 196, 240, 380, 1320, 1518
BlpI	GCTNAGC	1 cut(s)	1521
BlsI	GCNGC	7 cut(s)	116, 194, 197, 241, 381, 1321, 1519
Bme1390I	CCNGG	4 cut(s)	370, 722, 899, 1388
Bme18I	GGWCC	2 cut(s)	511, 718
BmeRI	GACNNNNNGTC	1 cut(s)	1246
BmeT110I	CYCGRG	2 cut(s)	200, 1019
BmgBI	CACGTC	2 cut(s)	277, 1243
BmgT120I	GGNCC	4 cut(s)	212, 258, 511, 718
BmiI	GGNNCC	6 cut(s)	214, 720, 726, 896, 1278, 1334
BmrFI	CCNGG	4 cut(s)	370, 722, 899, 1388
BmsI	GCATC	4 cut(s)	1171, 1331, 1450, 1571
BpiI	GAAGAC	1 cut(s)	225
BplI	GAGNNNNNCTC	2 cut(s)	1063, 1095
BpmI	CTGGAG	1 cut(s)	1419
Bpu1102I	GCTNAGC	1 cut(s)	1521
BpuEI	CTTGAG	2 cut(s)	1065, 1090
BpuMI	CCSGG	1 cut(s)	722
BsaBI	GATNNNNATC	1 cut(s)	306
BsaHI	GRCGYC	3 cut(s)	6, 78, 1277
BsaI	GGTCTC	1 cut(s)	470
BsaJI	CCNNGG	5 cut(s)	199, 369, 898, 1221, 1336
BsaWI	WCCGGW	1 cut(s)	478
BsaXI	ACNNNNNCTCC	2 cut(s)	344, 374
Bsc4I	CCNNNNNNNGG	4 cut(s)	369, 478, 508, 1212
Bse118I	RCCGGY	1 cut(s)	1368
Bse1I	ACTGG	3 cut(s)	147, 888, 1436
Bse21I	CCTNAGG	2 cut(s)	504, 1281
Bse3DI	GCAATG	1 cut(s)	553
Bse8I	GATNNNNATC	1 cut(s)	306
BseBI	CCWGG	3 cut(s)	370, 899, 1388
BseDI	CCNNGG	5 cut(s)	199, 369, 898, 1221, 1336
BseJI	GATNNNNATC	1 cut(s)	306
BseLI	CCNNNNNNNGG	4 cut(s)	369, 478, 508, 1212
BseMI	GCAATG	1 cut(s)	553
BseMII	CTCAG	4 cut(s)	332, 518, 1272, 1535
BseNI	ACTGG	3 cut(s)	147, 888, 1436
BseRI	GAGGAG	5 cut(s)	79, 97, 584, 1422, 1572
BseSI	GKGCMC	1 cut(s)	986
BseX3I	CGGCCG	1 cut(s)	115
BseXI	GCAGC	3 cut(s)	207, 391, 1331
BseYI	CCCAGC	2 cut(s)	252, 1573
Bsh1236I	CGCG	5 cut(s)	112, 114, 156, 327, 540
Bsh1285I	CGRYCG	2 cut(s)	46, 118
BshFI	GGCC	5 cut(s)	117, 213, 260, 1372, 1552
BshNI	GGYRCC	1 cut(s)	1276
BsiEI	CGRYCG	2 cut(s)	46, 118
BsiHKCI	CYCGRG	2 cut(s)	200, 1019
BsiSI	CCGG	4 cut(s)	210, 479, 722, 1369
BslFI	GGGAC	2 cut(s)	1273, 1556
BslI	CCNNNNNNNGG	4 cut(s)	369, 478, 508, 1212
BsmAI	GTCTC	2 cut(s)	470, 1090
BsmFI	GGGAC	2 cut(s)	1273, 1556
BsnI	GGCC	5 cut(s)	117, 213, 260, 1372, 1552
Bso31I	GGTCTC	1 cut(s)	470
BsoBI	CYCGRG	2 cut(s)	200, 1019
Bsp1286I	GDGCHC	1 cut(s)	986
Bsp143I	GATC	5 cut(s)	301, 670, 886, 1132, 1190
Bsp1720I	GCTNAGC	1 cut(s)	1521
BspANI	GGCC	5 cut(s)	117, 213, 260, 1372, 1552
BspCNI	CTCAG	4 cut(s)	331, 517, 1273, 1534
BspFNI	CGCG	5 cut(s)	112, 114, 156, 327, 540
BspHI	TCATGA	1 cut(s)	1129
BspLI	GGNNCC	6 cut(s)	214, 720, 726, 896, 1278, 1334
BspPI	GGATC	2 cut(s)	894, 1198
BspT107I	GGYRCC	1 cut(s)	1276
BspTNI	GGTCTC	1 cut(s)	470
BsrDI	GCAATG	1 cut(s)	553
BsrFI	RCCGGY	1 cut(s)	1368
BsrI	ACTGG	3 cut(s)	147, 888, 1436
BssAI	RCCGGY	1 cut(s)	1368
BssECI	CCNNGG	5 cut(s)	199, 369, 898, 1221, 1336
BssMI	GATC	5 cut(s)	301, 670, 886, 1132, 1190
BssNI	GRCGYC	3 cut(s)	6, 78, 1277
BssT1I	CCWWGG	2 cut(s)	1221, 1336
Bst2UI	CCWGG	3 cut(s)	370, 899, 1388
Bst4CI	ACNGT	3 cut(s)	389, 826, 1595
Bst6I	CTCTTC	1 cut(s)	561
BstACI	GRCGYC	3 cut(s)	6, 78, 1277
BstC8I	GCNNGC	8 cut(s)	258, 262, 473, 576, 1317, 1370, 1397, 1401
BstDEI	CTNAG	6 cut(s)	318, 504, 1002, 1281, 1295, 1521
BstFNI	CGCG	5 cut(s)	112, 114, 156, 327, 540
BstH2I	RGCGCY	1 cut(s)	1280
BstHHI	GCGC	4 cut(s)	114, 327, 1279, 1346
BstKTI	GATC	5 cut(s)	304, 673, 889, 1135, 1193
BstMAI	GTCTC	2 cut(s)	470, 1090
BstMBI	GATC	5 cut(s)	301, 670, 886, 1132, 1190
BstMCI	CGRYCG	2 cut(s)	46, 118
BstMWI	GCNNNNNNNGC	7 cut(s)	162, 171, 248, 257, 552, 584, 1341
BstNI	CCWGG	3 cut(s)	370, 899, 1388
BstNSI	RCATGY	1 cut(s)	1399
BstSCI	CCNGG	4 cut(s)	368, 720, 897, 1386
BstSLI	GKGCMC	1 cut(s)	986
BstUI	CGCG	5 cut(s)	112, 114, 156, 327, 540
BstV1I	GCAGC	3 cut(s)	207, 391, 1331
BstV2I	GAAGAC	1 cut(s)	225
BstX2I	RGATCY	1 cut(s)	1190
BstYI	RGATCY	1 cut(s)	1190
BstZI	CGGCCG	1 cut(s)	115
Bsu36I	CCTNAGG	2 cut(s)	504, 1281
BsuRI	GGCC	5 cut(s)	117, 213, 260, 1372, 1552
BtgZI	GCGATG	1 cut(s)	1473
BtrI	CACGTC	2 cut(s)	277, 1243
BtsI	GCAGTG	2 cut(s)	914, 1585
BtsIMutI	CAGTG	5 cut(s)	394, 822, 840, 914, 1585
Cac8I	GCNNGC	8 cut(s)	258, 262, 473, 576, 1317, 1370, 1397, 1401
CaiI	CAGNNNCTG	2 cut(s)	1442, 1528
CciI	TCATGA	1 cut(s)	1129
CfoI	GCGC	4 cut(s)	114, 327, 1279, 1346
Cfr10I	RCCGGY	1 cut(s)	1368
Cfr13I	GGNCC	4 cut(s)	212, 258, 511, 718
CseI	GACGC	2 cut(s)	86, 145
CviAII	CATG	3 cut(s)	959, 1130, 1396
DdeI	CTNAG	6 cut(s)	318, 504, 1002, 1281, 1295, 1521
DinI	GGCGCC	1 cut(s)	1278
DpnI	GATC	5 cut(s)	303, 672, 888, 1134, 1192
DpnII	GATC	5 cut(s)	301, 670, 886, 1132, 1190
DraI	TTTAAA	1 cut(s)	22
DriI	GACNNNNNGTC	1 cut(s)	1246
EaeI	YGGCCR	1 cut(s)	115
EagI	CGGCCG	1 cut(s)	115
Eam1104I	CTCTTC	1 cut(s)	561
Eam1105I	GACNNNNNGTC	1 cut(s)	1246
EarI	CTCTTC	1 cut(s)	561
EciI	GGCGGA	2 cut(s)	458, 781
EclXI	CGGCCG	1 cut(s)	115
Eco130I	CCWWGG	2 cut(s)	1221, 1336
Eco31I	GGTCTC	1 cut(s)	470
Eco32I	GATATC	1 cut(s)	1156
Eco47I	GGWCC	2 cut(s)	511, 718
Eco52I	CGGCCG	1 cut(s)	115
Eco81I	CCTNAGG	2 cut(s)	504, 1281
Eco88I	CYCGRG	2 cut(s)	200, 1019
EcoRI	GAATTC	1 cut(s)	176
EcoRII	CCWGG	3 cut(s)	368, 897, 1386
EcoRV	GATATC	1 cut(s)	1156
EcoT14I	CCWWGG	2 cut(s)	1221, 1336
EgeI	GGCGCC	1 cut(s)	1278
EheI	GGCGCC	1 cut(s)	1278
ErhI	CCWWGG	2 cut(s)	1221, 1336
FaeI	CATG	3 cut(s)	962, 1133, 1399
FaqI	GGGAC	2 cut(s)	1273, 1556
FatI	CATG	3 cut(s)	958, 1129, 1395
FauI	CCCGC	4 cut(s)	249, 480, 1106, 1373
FauNDI	CATATG	1 cut(s)	1328
FbaI	TGATCA	1 cut(s)	670
FblI	GTMKAC	2 cut(s)	69, 1507
Fnu4HI	GCNGC	7 cut(s)	115, 193, 196, 240, 380, 1320, 1518
Fsp4HI	GCNGC	7 cut(s)	115, 193, 196, 240, 380, 1320, 1518
GlaI	GCGC	4 cut(s)	113, 326, 1278, 1345
GluI	GCNGC	7 cut(s)	115, 193, 196, 240, 380, 1320, 1518
GsaI	CCCAGC	2 cut(s)	256, 1577
GsuI	CTGGAG	1 cut(s)	1419
HaeII	RGCGCY	1 cut(s)	1280
HaeIII	GGCC	5 cut(s)	117, 213, 260, 1372, 1552
HapII	CCGG	4 cut(s)	210, 479, 722, 1369
HgaI	GACGC	2 cut(s)	86, 145
HhaI	GCGC	4 cut(s)	114, 327, 1279, 1346
Hin1I	GRCGYC	3 cut(s)	6, 78, 1277
Hin1II	CATG	3 cut(s)	962, 1133, 1399
Hin6I	GCGC	4 cut(s)	112, 325, 1277, 1344
HinP1I	GCGC	4 cut(s)	112, 325, 1277, 1344
HincII	GTYRAC	6 cut(s)	70, 343, 1119, 1362, 1495, 1508
HindII	GTYRAC	6 cut(s)	70, 343, 1119, 1362, 1495, 1508
HinfI	GANTC	8 cut(s)	307, 535, 602, 769, 852, 1076, 1300, 1445
HpaI	GTTAAC	2 cut(s)	1362, 1495
HpaII	CCGG	4 cut(s)	210, 479, 722, 1369
HphI	GGTGA	4 cut(s)	271, 738, 1376, 1436
Hpy188I	TCNGA	7 cut(s)	30, 161, 306, 364, 562, 1059, 1413
Hpy188III	TCNNGA	4 cut(s)	506, 1130, 1442, 1605
Hpy99I	CGWCG	3 cut(s)	11, 71, 80
HpyAV	CCTTC	3 cut(s)	105, 827, 1385
HpyCH4III	ACNGT	3 cut(s)	389, 826, 1595
HpyCH4IV	ACGT	7 cut(s)	6, 25, 66, 276, 339, 345, 1242
HpyF10VI	GCNNNNNNNGC	7 cut(s)	162, 171, 248, 257, 552, 584, 1341
HpyF3I	CTNAG	6 cut(s)	318, 504, 1002, 1281, 1295, 1521
HpySE526I	ACGT	7 cut(s)	6, 25, 66, 276, 339, 345, 1242
Hsp92I	GRCGYC	3 cut(s)	6, 78, 1277
Hsp92II	CATG	3 cut(s)	962, 1133, 1399
HspAI	GCGC	4 cut(s)	112, 325, 1277, 1344
KasI	GGCGCC	1 cut(s)	1276
KroI	GCCGGC	1 cut(s)	1368
KroNI	GCCGGC	1 cut(s)	1370
Ksp22I	TGATCA	1 cut(s)	670
KspAI	GTTAAC	2 cut(s)	1362, 1495
Kzo9I	GATC	5 cut(s)	301, 670, 886, 1132, 1190
LmnI	GCTCC	4 cut(s)	571, 724, 894, 1332
Lsp1109I	GCAGC	3 cut(s)	207, 391, 1331
LweI	GCATC	4 cut(s)	1171, 1331, 1450, 1571
MaeII	ACGT	7 cut(s)	6, 25, 66, 276, 339, 345, 1242
MaeIII	GTNAC	3 cut(s)	119, 277, 1027
MalI	GATC	5 cut(s)	303, 672, 888, 1134, 1192
MboI	GATC	5 cut(s)	301, 670, 886, 1132, 1190
MboII	GAAGA	6 cut(s)	230, 367, 444, 578, 1006, 1118
MflI	RGATCY	1 cut(s)	1190
MhlI	GDGCHC	1 cut(s)	986
MluCI	AATT	7 cut(s)	17, 176, 416, 456, 526, 758, 1232
Mly113I	GGCGCC	1 cut(s)	1277
MlyI	GAGTC	1 cut(s)	778
MmeI	TCCRAC	2 cut(s)	663, 943
MroNI	GCCGGC	1 cut(s)	1368
MroXI	GAANNNNTTC	1 cut(s)	811
MseI	TTAA	9 cut(s)	21, 60, 459, 944, 1109, 1236, 1290, 1361, 1494
MslI	CAYNNNNRTG	1 cut(s)	519
MspA1I	CMGCKG	2 cut(s)	256, 1322
MspI	CCGG	4 cut(s)	210, 479, 722, 1369
MspR9I	CCNGG	4 cut(s)	370, 722, 899, 1388
MvaI	CCWGG	3 cut(s)	370, 899, 1388
MvnI	CGCG	5 cut(s)	112, 114, 156, 327, 540
MwoI	GCNNNNNNNGC	7 cut(s)	162, 171, 248, 257, 552, 584, 1341
NaeI	GCCGGC	1 cut(s)	1370
NarI	GGCGCC	1 cut(s)	1277
NciI	CCSGG	1 cut(s)	722
NdeI	CATATG	1 cut(s)	1328
NdeII	GATC	5 cut(s)	301, 670, 886, 1132, 1190
NgoMIV	GCCGGC	1 cut(s)	1368
NlaIII	CATG	3 cut(s)	962, 1133, 1399
NlaIV	GGNNCC	6 cut(s)	214, 720, 726, 896, 1278, 1334
NmuCI	GTSAC	2 cut(s)	119, 277
NspI	RCATGY	1 cut(s)	1399
OliI	CACNNNNGTG	1 cut(s)	519
PaeI	GCATGC	1 cut(s)	1399
PaeR7I	CTCGAG	1 cut(s)	1019
PagI	TCATGA	1 cut(s)	1129
PcsI	WCGNNNNNNNCGW	2 cut(s)	72, 177
PdiI	GCCGGC	1 cut(s)	1370
PdmI	GAANNNNTTC	1 cut(s)	811
PfeI	GAWTC	7 cut(s)	307, 535, 602, 852, 1076, 1300, 1445
PkrI	GCNGC	7 cut(s)	116, 194, 197, 241, 381, 1321, 1519
PleI	GAGTC	1 cut(s)	777
PluTI	GGCGCC	1 cut(s)	1280
PpsI	GAGTC	1 cut(s)	777
Psp1406I	AACGTT	1 cut(s)	339
Psp6I	CCWGG	3 cut(s)	368, 897, 1386
PspFI	CCCAGC	2 cut(s)	252, 1573
PspGI	CCWGG	3 cut(s)	368, 897, 1386
PspN4I	GGNNCC	6 cut(s)	214, 720, 726, 896, 1278, 1334
PspPI	GGNCC	4 cut(s)	212, 258, 511, 718
PspXI	VCTCGAGB	1 cut(s)	1019
PsrI	GAACNNNNNNTAC	2 cut(s)	56, 88
PstNI	CAGNNNCTG	2 cut(s)	1442, 1528
PsuI	RGATCY	1 cut(s)	1190
PvuII	CAGCTG	1 cut(s)	1322
RseI	CAYNNNNRTG	1 cut(s)	519
SalI	GTCGAC	2 cut(s)	68, 1506
SaqAI	TTAA	9 cut(s)	21, 60, 459, 944, 1109, 1236, 1290, 1361, 1494
SatI	GCNGC	7 cut(s)	115, 193, 196, 240, 380, 1320, 1518
Sau3AI	GATC	5 cut(s)	301, 670, 886, 1132, 1190
Sau96I	GGNCC	4 cut(s)	212, 258, 511, 718
SchI	GAGTC	1 cut(s)	778
ScrFI	CCNGG	4 cut(s)	370, 722, 899, 1388
SduI	GDGCHC	1 cut(s)	986
SfaNI	GCATC	4 cut(s)	1171, 1331, 1450, 1571
SfoI	GGCGCC	1 cut(s)	1278
Sfr274I	CTCGAG	1 cut(s)	1019
SinI	GGWCC	2 cut(s)	511, 718
SlaI	CTCGAG	1 cut(s)	1019
SmiMI	CAYNNNNRTG	1 cut(s)	519
SmlI	CTYRAG	3 cut(s)	1019, 1069, 1080
SmoI	CTYRAG	3 cut(s)	1019, 1069, 1080
SphI	GCATGC	1 cut(s)	1399
Sse9I	AATT	7 cut(s)	17, 176, 416, 456, 526, 758, 1232
SspDI	GGCGCC	1 cut(s)	1276
StyD4I	CCNGG	4 cut(s)	368, 720, 897, 1386
StyI	CCWWGG	2 cut(s)	1221, 1336
TaaI	ACNGT	3 cut(s)	389, 826, 1595
TaiI	ACGT	7 cut(s)	9, 28, 69, 279, 342, 348, 1245
TaqI	TCGA	7 cut(s)	42, 69, 180, 384, 404, 1020, 1507
TasI	AATT	7 cut(s)	17, 176, 416, 456, 526, 758, 1232
TauI	GCSGC	4 cut(s)	117, 195, 242, 1520
TfiI	GAWTC	7 cut(s)	307, 535, 602, 852, 1076, 1300, 1445
Tru1I	TTAA	9 cut(s)	21, 60, 459, 944, 1109, 1236, 1290, 1361, 1494
Tru9I	TTAA	9 cut(s)	21, 60, 459, 944, 1109, 1236, 1290, 1361, 1494
TscAI	CASTG	5 cut(s)	394, 829, 847, 914, 1592
TseFI	GTSAC	2 cut(s)	119, 277
TseI	GCWGC	3 cut(s)	195, 379, 1319
Tsp45I	GTSAC	2 cut(s)	119, 277
TspDTI	ATGAA	7 cut(s)	579, 597, 846, 1011, 1118, 1462, 1589
TspRI	CASTG	5 cut(s)	394, 829, 847, 914, 1592
VpaK11BI	GGWCC	2 cut(s)	511, 718
XapI	RAATTY	5 cut(s)	176, 416, 526, 758, 1232
XceI	RCATGY	1 cut(s)	1399
XcmI	CCANNNNNNNNNTGG	1 cut(s)	240
XhoI	CTCGAG	1 cut(s)	1019
XmiI	GTMKAC	2 cut(s)	69, 1507
XmnI	GAANNNNTTC	1 cut(s)	811
ZraI	GACGTC	1 cut(s)	7

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.