RLG00000011859

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000243
Physical Location & Seq
Forward (+)
13365146 .. 13371284
6139 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011859

Sequence Viewer

Length: 1506 bp
ATGACTCCCATATTCTTTGGTCCCCATTACGACTGCGAATGTGAGGAAGACTACGCTGCTGAATATGTACCAAGTAGAGACGAAATTGAATGCATTCCCAGCACCAAAAAGAAGAAAGACCAAATTGGGTGCATTGGATATTTCTCCAAAAACCTGGACAAAGGGTGCGCTTCTTGGTCGTGGCTCATTCGGCTCTGTATATCAAGGATCTTAGATGGATTCATTTTCGCTGCCACGGAAGTTTCATTGCTTGATCAAGGAAGTCAGGGGAAGAGCTGTGTTTCTCAACTTCAACAGGAGATTGAGTTTCTCAGTTGGCATGAACACAAAAACATAGTTCAATACTATGGCTCATTCGAGGGTAAATTTGCTAATCAAGCAGTAGATCTTAACAGCCTCTTGTTTGTGTGCATCATAGTCTTATTTATTTTATCTAAATGGTCTTTACAATATGGATCAAATCTTTATATGTGTATGGAGCATGCACCTAATGGATCCATTCGACAACTCTATCAGAGATTGAATCTTTCACTTCCTCGTGTCTCTGAATATACAAAAGAGATCCTTCTTGGTCTAAAGTATCTTCATGACCATAATGTGGTTCACAGGGACATCAAATGTGCAAATATTTTAGTGGATGCTTATGGATCTGCAAAGCTTGCAGATTTTGGATTGGCAAAGATAACCACCAAAATGAATGAAATTCAATCTTTGCAGGGGACTGCATTCTGGATGGCCCCTGAGGTTCTTAGTGCAAAAATGAAGAACCAAGGTTATGGACCTCCAGCGGATATATGGAGCCTTGGTTGCACGGTGTTGGAGATGTTAACAAGTTTCAGGCATGCATGTTTTAAGATTGCAATGGGGGAGCTCCCTCACATTCCTCATTTTCATTGTAAGGAGGTGCGAGATTTCATCCGTCAGTGCCAACAAGTTAATCCAAACTATCGACCCACTGCTGCTCAGCTCTTATGTCATCCATTTCTCATGTCTGGACCTATCAAACTACCCCATTTTGGAGATGGTCTTTGTATGCGGATGAAGGAAAAGAACTTTATGCAAGAGGAGATTGTTGATGGTAGGGAAGTCGCTAGGGATGATAAAGTACTCTATCGTGTCATTGAACTGCGTCCAAATCAAGATACCGGCGCTTGCAACCGTTCGTCGTCAAATCCACCTTTCACGGTTCCACCAATCAAGTCTTGCCCAAACAGTAACTGCGTCAGACTGACGGTCATAGCTTCGATCAGGCAAATGACCCAGTCGACGACGGCGTTGGCGGTTCTATCTCGCTGTTGTTGTATCGTTTGCCGGACGGGAATGATTTTGGGCTACTACCCTATTGGCTTAGGGATTTATGGCCTAAGGTTTTACTATGGTGGCGGCGTAGTTCAGGATCTTTCTTCTCTACGCCATGGAGGCCATGGACATCATCTCGAGGGAGGTGATTCACAGCGTGAGGTGGGTTTCGGCGGCGGGGATGGCAACATCGGGGGGAGTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

502

Amino Acids

56.16

Weight (kDa)

7.48

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 86 - 329 5.2e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 152 - 324 1.9e-27 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 598
AccI GTMKAC 1 cut(s) 1265
AciI CCGC 6 cut(s) 788, 1036, 1280, 1383, 1473, 1476
AclWI GGATC 7 cut(s) 215, 463, 489, 502, 556, 655, 1404
AcsI RAATTY 2 cut(s) 365, 702
AdeI CACNNNGTG 1 cut(s) 1457
AfaI GTAC 2 cut(s) 69, 1107
AfiI CCNNNNNNNGG 2 cut(s) 598, 1016
AgsI TTSAA 6 cut(s) 89, 293, 341, 523, 707, 1124
AhdI GACNNNNNGTC 1 cut(s) 1232
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 5 cut(s) 276, 658, 871, 967, 1241
AluI AGCT 5 cut(s) 276, 658, 871, 967, 1241
Alw21I GWGCWC 1 cut(s) 873
Alw26I GTCTC 2 cut(s) 72, 547
AlwI GGATC 7 cut(s) 215, 463, 489, 502, 556, 655, 1404
AlwNI CAGNNNCTG 1 cut(s) 1218
Ama87I CYCGRG 1 cut(s) 1436
AoxI GGCC 3 cut(s) 735, 1360, 1420
ApeKI GCWGC 3 cut(s) 56, 230, 959
ApoI RAATTY 2 cut(s) 365, 702
Asp700I GAANNNNTTC 1 cut(s) 93
AspLEI GCGC 2 cut(s) 170, 1151
AspS9I GGNCC 4 cut(s) 20, 736, 779, 995
AsuHPI GGTGA 1 cut(s) 1457
AvaI CYCGRG 1 cut(s) 1436
AvaII GGWCC 3 cut(s) 20, 779, 995
AxyI CCTNAGG 2 cut(s) 741, 1364
BamHI GGATCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 873
BauI CACGAG 1 cut(s) 537
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 873
BbvI GCAGC 3 cut(s) 43, 217, 946
BccI CCATC 5 cut(s) 209, 727, 1016, 1070, 1475
BceAI ACGGC 1 cut(s) 1287
BciT130I CCWGG 1 cut(s) 155
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 72, 547
BfaI CTAG 1 cut(s) 1092
BfoI RGCGCY 1 cut(s) 1152
BglI GCCNNNNNGGC 1 cut(s) 1419
BglII AGATCT 1 cut(s) 385
BisI GCNGC 5 cut(s) 57, 231, 960, 1384, 1474
BlpI GCTNAGC 1 cut(s) 963
BlsI GCNGC 5 cut(s) 58, 232, 961, 1385, 1475
BmcAI AGTACT 1 cut(s) 1107
Bme1390I CCNGG 1 cut(s) 155
Bme18I GGWCC 3 cut(s) 20, 779, 995
BmeRI GACNNNNNGTC 1 cut(s) 1232
BmeT110I CYCGRG 1 cut(s) 1436
BmgT120I GGNCC 4 cut(s) 20, 736, 779, 995
BmiI GGNNCC 5 cut(s) 22, 496, 738, 800, 1188
BmrFI CCNGG 1 cut(s) 155
BmrI ACTGGG 1 cut(s) 1255
BmsI GCATC 2 cut(s) 420, 628
BmuI ACTGGG 1 cut(s) 1255
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 768
Bpu10I CCTNAGC 1 cut(s) 1348
Bpu1102I GCTNAGC 1 cut(s) 963
BsaJI CCNNGG 5 cut(s) 234, 769, 802, 1414, 1423
BsaXI ACNNNNNCTCC 4 cut(s) 290, 320, 790, 820
Bsc4I CCNNNNNNNGG 2 cut(s) 598, 1016
Bse118I RCCGGY 1 cut(s) 1145
Bse1I ACTGG 1 cut(s) 1261
Bse21I CCTNAGG 2 cut(s) 741, 1364
Bse3DI GCAATG 2 cut(s) 245, 867
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 5 cut(s) 234, 769, 802, 1414, 1423
BseGI GGATG 7 cut(s) 643, 738, 915, 976, 1044, 1102, 1486
BseLI CCNNNNNNNGG 2 cut(s) 598, 1016
BseMI GCAATG 2 cut(s) 245, 867
BseMII CTCAG 3 cut(s) 325, 732, 977
BseNI ACTGG 1 cut(s) 1261
BseRI GAGGAG 1 cut(s) 1079
BseXI GCAGC 3 cut(s) 43, 217, 946
BseYI CCCAGC 1 cut(s) 98
BshFI GGCC 3 cut(s) 737, 1362, 1422
BsiHKAI GWGCWC 1 cut(s) 873
BsiHKCI CYCGRG 1 cut(s) 1436
BsiSI CCGG 2 cut(s) 1146, 1312
BslFI GGGAC 3 cut(s) 6, 623, 733
BslI CCNNNNNNNGG 2 cut(s) 598, 1016
BsmAI GTCTC 2 cut(s) 72, 547
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 3 cut(s) 6, 623, 733
BsmI GAATGC 3 cut(s) 93, 95, 725
BsnI GGCC 3 cut(s) 737, 1362, 1422
BsoBI CYCGRG 1 cut(s) 1436
Bsp1286I GDGCHC 1 cut(s) 873
Bsp143I GATC 9 cut(s) 207, 253, 385, 455, 494, 561, 647, 1245, 1396
Bsp1720I GCTNAGC 1 cut(s) 963
Bsp19I CCATGG 2 cut(s) 1414, 1423
BspACI CCGC 6 cut(s) 788, 1036, 1280, 1383, 1473, 1476
BspANI GGCC 3 cut(s) 737, 1362, 1422
BspCNI CTCAG 3 cut(s) 324, 733, 976
BspHI TCATGA 1 cut(s) 586
BspLI GGNNCC 5 cut(s) 22, 496, 738, 800, 1188
BspPI GGATC 7 cut(s) 215, 463, 489, 502, 556, 655, 1404
BspQI GCTCTTC 1 cut(s) 266
BsrDI GCAATG 2 cut(s) 245, 867
BsrFI RCCGGY 1 cut(s) 1145
BsrI ACTGG 1 cut(s) 1261
BssAI RCCGGY 1 cut(s) 1145
BssECI CCNNGG 5 cut(s) 234, 769, 802, 1414, 1423
BssMI GATC 9 cut(s) 207, 253, 385, 455, 494, 561, 647, 1245, 1396
BssSI CACGAG 1 cut(s) 537
BssT1I CCWWGG 4 cut(s) 769, 802, 1414, 1423
Bst2BI CACGAG 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 5 cut(s) 814, 1160, 1186, 1214, 1234
Bst6I CTCTTC 1 cut(s) 266
BstAPI GCANNNNNTGC 1 cut(s) 659
BstC8I GCNNGC 4 cut(s) 483, 660, 843, 1153
BstDEI CTNAG 7 cut(s) 211, 311, 741, 749, 963, 1348, 1364
BstDSI CCRYGG 3 cut(s) 234, 1414, 1423
BstF5I GGATG 7 cut(s) 643, 738, 915, 976, 1044, 1102, 1486
BstH2I RGCGCY 1 cut(s) 1152
BstHHI GCGC 2 cut(s) 170, 1151
BstKTI GATC 9 cut(s) 210, 256, 388, 458, 497, 564, 650, 1248, 1399
BstMAI GTCTC 2 cut(s) 72, 547
BstMBI GATC 9 cut(s) 207, 253, 385, 455, 494, 561, 647, 1245, 1396
BstMWI GCNNNNNNNGC 7 cut(s) 99, 190, 377, 659, 807, 1419, 1482
BstNI CCWGG 1 cut(s) 155
BstNSI RCATGY 3 cut(s) 485, 845, 849
BstSCI CCNGG 1 cut(s) 153
BstV1I GCAGC 3 cut(s) 43, 217, 946
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 6 cut(s) 207, 385, 494, 561, 647, 1396
BstXI CCANNNNNNTGG 2 cut(s) 154, 776
BstYI RGATCY 6 cut(s) 207, 385, 494, 561, 647, 1396
Bsu36I CCTNAGG 2 cut(s) 741, 1364
BsuRI GGCC 3 cut(s) 737, 1362, 1422
BtgI CCRYGG 3 cut(s) 234, 1414, 1423
BtsCI GGATG 7 cut(s) 643, 738, 915, 976, 1044, 1102, 1486
BtsI GCAGTG 1 cut(s) 954
BtsIMutI CAGTG 2 cut(s) 929, 954
Cac8I GCNNGC 4 cut(s) 483, 660, 843, 1153
CaiI CAGNNNCTG 1 cut(s) 1218
CciI TCATGA 1 cut(s) 586
CfoI GCGC 2 cut(s) 170, 1151
Cfr10I RCCGGY 1 cut(s) 1145
Cfr13I GGNCC 4 cut(s) 20, 736, 779, 995
CseI GACGC 2 cut(s) 1118, 1210
Csp6I GTAC 2 cut(s) 68, 1106
CviAII CATG 8 cut(s) 320, 482, 587, 842, 846, 988, 1415, 1424
CviQI GTAC 2 cut(s) 68, 1106
DdeI CTNAG 7 cut(s) 211, 311, 741, 749, 963, 1348, 1364
DpnI GATC 9 cut(s) 209, 255, 387, 457, 496, 563, 649, 1247, 1398
DpnII GATC 9 cut(s) 207, 253, 385, 455, 494, 561, 647, 1245, 1396
DraIII CACNNNGTG 1 cut(s) 1457
DriI GACNNNNNGTC 1 cut(s) 1232
Eam1104I CTCTTC 1 cut(s) 266
Eam1105I GACNNNNNGTC 1 cut(s) 1232
EarI CTCTTC 1 cut(s) 266
Ecl136II GAGCTC 1 cut(s) 871
Eco130I CCWWGG 4 cut(s) 769, 802, 1414, 1423
Eco24I GRGCYC 1 cut(s) 873
Eco47I GGWCC 3 cut(s) 20, 779, 995
Eco53kI GAGCTC 1 cut(s) 871
Eco81I CCTNAGG 2 cut(s) 741, 1364
Eco88I CYCGRG 1 cut(s) 1436
EcoICRI GAGCTC 1 cut(s) 871
EcoRII CCWGG 1 cut(s) 153
EcoT14I CCWWGG 4 cut(s) 769, 802, 1414, 1423
EcoT22I ATGCAT 2 cut(s) 95, 847
EcoT38I GRGCYC 1 cut(s) 873
ErhI CCWWGG 4 cut(s) 769, 802, 1414, 1423
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 8 cut(s) 323, 485, 590, 845, 849, 991, 1418, 1427
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 3 cut(s) 6, 623, 733
FatI CATG 8 cut(s) 319, 481, 586, 841, 845, 987, 1414, 1423
FauI CCCGC 1 cut(s) 1469
FbaI TGATCA 1 cut(s) 253
FblI GTMKAC 1 cut(s) 1265
Fnu4HI GCNGC 5 cut(s) 57, 231, 960, 1384, 1474
FokI GGATG 7 cut(s) 650, 745, 902, 963, 1051, 1109, 1493
FriOI GRGCYC 1 cut(s) 873
Fsp4HI GCNGC 5 cut(s) 57, 231, 960, 1384, 1474
FspBI CTAG 1 cut(s) 1092
GlaI GCGC 2 cut(s) 169, 1150
GluI GCNGC 5 cut(s) 57, 231, 960, 1384, 1474
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 1 cut(s) 768
HaeII RGCGCY 1 cut(s) 1152
HaeIII GGCC 3 cut(s) 737, 1362, 1422
HapII CCGG 2 cut(s) 1146, 1312
HgaI GACGC 2 cut(s) 1118, 1210
HhaI GCGC 2 cut(s) 170, 1151
Hin1II CATG 8 cut(s) 323, 485, 590, 845, 849, 991, 1418, 1427
Hin6I GCGC 2 cut(s) 168, 1149
HinP1I GCGC 2 cut(s) 168, 1149
HincII GTYRAC 2 cut(s) 828, 1266
HindII GTYRAC 2 cut(s) 828, 1266
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 4 cut(s) 4, 219, 523, 1448
HpaI GTTAAC 1 cut(s) 828
HpaII CCGG 2 cut(s) 1146, 1312
HphI GGTGA 1 cut(s) 1457
Hpy166II GTNNAC 3 cut(s) 604, 828, 1266
Hpy188I TCNGA 3 cut(s) 516, 547, 1226
Hpy188III TCNNGA 6 cut(s) 587, 730, 993, 1139, 1394, 1436
Hpy8I GTNNAC 3 cut(s) 604, 828, 1266
Hpy99I CGWCG 3 cut(s) 1168, 1270, 1273
HpyAV CCTTC 2 cut(s) 575, 1036
HpyCH4III ACNGT 5 cut(s) 814, 1160, 1186, 1214, 1234
HpyF10VI GCNNNNNNNGC 7 cut(s) 99, 190, 377, 659, 807, 1419, 1482
HpyF3I CTNAG 7 cut(s) 211, 311, 741, 749, 963, 1348, 1364
Hsp92II CATG 8 cut(s) 323, 485, 590, 845, 849, 991, 1418, 1427
HspAI GCGC 2 cut(s) 168, 1149
Ksp22I TGATCA 1 cut(s) 253
KspAI GTTAAC 1 cut(s) 828
Kzo9I GATC 9 cut(s) 207, 253, 385, 455, 494, 561, 647, 1245, 1396
LguI GCTCTTC 1 cut(s) 266
LmnI GCTCC 4 cut(s) 478, 798, 868, 876
Lsp1109I GCAGC 3 cut(s) 43, 217, 946
LweI GCATC 2 cut(s) 420, 628
MaeI CTAG 1 cut(s) 1092
MaeIII GTNAC 1 cut(s) 1214
MalI GATC 9 cut(s) 209, 255, 387, 457, 496, 563, 649, 1247, 1398
MboI GATC 9 cut(s) 207, 253, 385, 455, 494, 561, 647, 1245, 1396
MboII GAAGA 6 cut(s) 59, 124, 283, 575, 775, 1395
MflI RGATCY 6 cut(s) 207, 385, 494, 561, 647, 1396
MhlI GDGCHC 1 cut(s) 873
MluCI AATT 4 cut(s) 84, 123, 365, 702
MmeI TCCRAC 1 cut(s) 798
Mph1103I ATGCAT 2 cut(s) 95, 847
MroXI GAANNNNTTC 1 cut(s) 93
MseI TTAA 4 cut(s) 390, 827, 852, 936
MslI CAYNNNNRTG 1 cut(s) 692
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 2 cut(s) 1146, 1312
MspR9I CCNGG 1 cut(s) 155
Mva1269I GAATGC 3 cut(s) 93, 95, 725
MvaI CCWGG 1 cut(s) 155
MwoI GCNNNNNNNGC 7 cut(s) 99, 190, 377, 659, 807, 1419, 1482
NcoI CCATGG 2 cut(s) 1414, 1423
NdeII GATC 9 cut(s) 207, 253, 385, 455, 494, 561, 647, 1245, 1396
NlaIII CATG 8 cut(s) 323, 485, 590, 845, 849, 991, 1418, 1427
NlaIV GGNNCC 5 cut(s) 22, 496, 738, 800, 1188
NsiI ATGCAT 2 cut(s) 95, 847
NspI RCATGY 3 cut(s) 485, 845, 849
PaeI GCATGC 2 cut(s) 485, 845
PaeR7I CTCGAG 1 cut(s) 1436
PagI TCATGA 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 1271
PctI GAATGC 3 cut(s) 93, 95, 725
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 219, 523, 1448
PflFI GACNNNGTC 1 cut(s) 1261
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 5 cut(s) 58, 232, 961, 1385, 1475
Psp124BI GAGCTC 1 cut(s) 873
Psp6I CCWGG 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 5 cut(s) 22, 496, 738, 800, 1188
PspPI GGNCC 4 cut(s) 20, 736, 779, 995
PstNI CAGNNNCTG 1 cut(s) 1218
PsuI RGATCY 6 cut(s) 207, 385, 494, 561, 647, 1396
PsyI GACNNNGTC 1 cut(s) 1261
RsaI GTAC 2 cut(s) 69, 1107
RsaNI GTAC 2 cut(s) 68, 1106
RseI CAYNNNNRTG 1 cut(s) 692
SacI GAGCTC 1 cut(s) 873
SalI GTCGAC 1 cut(s) 1264
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 4 cut(s) 390, 827, 852, 936
SatI GCNGC 5 cut(s) 57, 231, 960, 1384, 1474
Sau3AI GATC 9 cut(s) 207, 253, 385, 455, 494, 561, 647, 1245, 1396
Sau96I GGNCC 4 cut(s) 20, 736, 779, 995
ScaI AGTACT 1 cut(s) 1107
ScrFI CCNGG 1 cut(s) 155
SduI GDGCHC 1 cut(s) 873
SfaNI GCATC 2 cut(s) 420, 628
Sfr274I CTCGAG 1 cut(s) 1436
SinI GGWCC 3 cut(s) 20, 779, 995
SlaI CTCGAG 1 cut(s) 1436
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 1436
SmoI CTYRAG 1 cut(s) 1436
SphI GCATGC 2 cut(s) 485, 845
Sse9I AATT 4 cut(s) 84, 123, 365, 702
SsiI CCGC 6 cut(s) 788, 1036, 1280, 1383, 1473, 1476
SspI AATATT 1 cut(s) 628
SspMI CTAG 1 cut(s) 1092
SstI GAGCTC 1 cut(s) 873
StyD4I CCNGG 1 cut(s) 153
StyI CCWWGG 4 cut(s) 769, 802, 1414, 1423
TaaI ACNGT 5 cut(s) 814, 1160, 1186, 1214, 1234
TaqI TCGA 6 cut(s) 357, 502, 949, 1244, 1265, 1437
TasI AATT 4 cut(s) 84, 123, 365, 702
TatI WGTACW 1 cut(s) 1105
TauI GCSGC 2 cut(s) 1386, 1476
TfiI GAWTC 3 cut(s) 219, 523, 1448
Tru1I TTAA 4 cut(s) 390, 827, 852, 936
Tru9I TTAA 4 cut(s) 390, 827, 852, 936
TscAI CASTG 2 cut(s) 929, 961
TseI GCWGC 3 cut(s) 56, 230, 959
TspGWI ACGGA 2 cut(s) 251, 908
TspRI CASTG 2 cut(s) 929, 961
Tth111I GACNNNGTC 1 cut(s) 1261
Van91I CCANNNNNTGG 1 cut(s) 598
VpaK11BI GGWCC 3 cut(s) 20, 779, 995
XapI RAATTY 2 cut(s) 365, 702
XceI RCATGY 3 cut(s) 485, 845, 849
XcmI CCANNNNNNNNNTGG 3 cut(s) 792, 1019, 1421
XhoI CTCGAG 1 cut(s) 1436
XmiI GTMKAC 1 cut(s) 1265
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 1 cut(s) 1092
ZrmI AGTACT 1 cut(s) 1107
Zsp2I ATGCAT 2 cut(s) 95, 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.