Rh5CG563700

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000243
Physical Location & Seq
Reverse (-)
79365180 .. 79397680
32501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG563700.1

Sequence Viewer

Length: 498 bp
ATGGTGAGGGTTCCGGTGATTGACAATCGATGCTCGACTTGGGACATTTTGAGGGACTTTGCGCCGGAAGAAGAGAGGGAATCGTCGGAAATGGTGCAGAGAAGGTTTGGTTCCTTTTCCTCGTCTTCTTCTGATGATGAAGAAGAAGTTGAAGAAGGAGAGGATGTTGACTTTTCCAGCACTACCGCAGAGATTTCCAGTATTTCTCCAAATGGAAGGTTTTCGCCGAATGGGAAGCCTAAGCTTACTGTCACTAGTTGGGAAAAGGGTGATTTGCTCGGCAGCGGCTCTTTTGGGTCTGTGTATGAAGGAATTTCTGATTGCGGAAGCTTCTTTGCTGTCAAGGAAGTTTCCTTGCATGATCAAGGAAGAGTTTCTCGACTTGAACAGCACTACTTTTATTTGGTCAATTGGTCTTATTGGGGACAAATTAACCCAACTGCCTTTGTCCCTGAATCTAAAGATGTGGTTCATTCTGCAGAACTCCGTAGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

165

Amino Acids

18.55

Weight (kDa)

4.59

Isoelectric Point (pI)

54.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 186, 285, 324
AcsI RAATTY 1 cut(s) 312
AgsI TTSAA 2 cut(s) 152, 386
AhlI ACTAGT 1 cut(s) 254
AluBI AGCT 2 cut(s) 244, 330
AluI AGCT 2 cut(s) 244, 330
ApeKI GCWGC 1 cut(s) 282
ApoI RAATTY 1 cut(s) 312
Asp700I GAANNNNTTC 2 cut(s) 220, 373
AspLEI GCGC 1 cut(s) 64
AsuHPI GGTGA 3 cut(s) 16, 28, 281
BbsI GAAGAC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 294
BclI TGATCA 1 cut(s) 361
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 477
BglII AGATCT 1 cut(s) 491
BisI GCNGC 2 cut(s) 283, 286
BlsI GCNGC 2 cut(s) 284, 287
BmiI GGNNCC 2 cut(s) 12, 112
BmsI GCATC 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 28
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bse1I ACTGG 1 cut(s) 198
BseCI ATCGAT 1 cut(s) 28
BseGI GGATG 1 cut(s) 169
BseNI ACTGG 1 cut(s) 198
BseXI GCAGC 1 cut(s) 294
BsgI GTGCAG 1 cut(s) 116
BshVI ATCGAT 1 cut(s) 28
BsiSI CCGG 2 cut(s) 14, 65
BslFI GGGAC 4 cut(s) 56, 68, 434, 438
BsmFI GGGAC 4 cut(s) 56, 68, 434, 438
Bsp143I GATC 2 cut(s) 361, 491
BspACI CCGC 3 cut(s) 186, 285, 324
BspDI ATCGAT 1 cut(s) 28
BspLI GGNNCC 2 cut(s) 12, 112
BspMAI CTGCAG 1 cut(s) 481
BsrI ACTGG 1 cut(s) 198
BssMI GATC 2 cut(s) 361, 491
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 2 cut(s) 66, 364
BstDEI CTNAG 1 cut(s) 240
BstF5I GGATG 1 cut(s) 169
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 2 cut(s) 364, 494
BstMBI GATC 2 cut(s) 361, 491
BstSFI CTRYAG 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 491
BstYI RGATCY 1 cut(s) 491
Bsu15I ATCGAT 1 cut(s) 28
BsuTUI ATCGAT 1 cut(s) 28
BtsCI GGATG 1 cut(s) 169
CfoI GCGC 1 cut(s) 64
ClaI ATCGAT 1 cut(s) 28
CviAII CATG 1 cut(s) 359
CviJI RGCY 4 cut(s) 238, 244, 288, 330
CviKI_1 RGCY 4 cut(s) 238, 244, 288, 330
DdeI CTNAG 1 cut(s) 240
DpnI GATC 2 cut(s) 363, 493
DpnII GATC 2 cut(s) 361, 491
Eam1104I CTCTTC 2 cut(s) 66, 364
EarI CTCTTC 2 cut(s) 66, 364
FaeI CATG 1 cut(s) 362
FaiI YATR 2 cut(s) 306, 360
FaqI GGGAC 4 cut(s) 56, 68, 434, 438
FatI CATG 1 cut(s) 358
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 2 cut(s) 283, 286
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 2 cut(s) 283, 286
FspBI CTAG 1 cut(s) 255
GlaI GCGC 1 cut(s) 63
GluI GCNGC 2 cut(s) 283, 286
HapII CCGG 2 cut(s) 14, 65
HhaI GCGC 1 cut(s) 64
Hin1II CATG 1 cut(s) 362
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 2 cut(s) 242, 328
HinfI GANTC 2 cut(s) 80, 455
HpaII CCGG 2 cut(s) 14, 65
HphI GGTGA 3 cut(s) 16, 28, 281
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 3 cut(s) 88, 133, 319
Hpy188III TCNNGA 2 cut(s) 378, 495
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 4 cut(s) 96, 149, 210, 302
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 3 cut(s) 97, 358, 479
HpyF3I CTNAG 1 cut(s) 240
Hsp92II CATG 1 cut(s) 362
HspAI GCGC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 2 cut(s) 361, 491
LpnPI CCDG 5 cut(s) 27, 78, 190, 211, 465
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 1 cut(s) 20
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 250
MalI GATC 2 cut(s) 363, 493
MboI GATC 2 cut(s) 361, 491
MboII GAAGA 8 cut(s) 80, 83, 117, 120, 152, 155, 164, 381
MfeI CAATTG 1 cut(s) 409
MflI RGATCY 1 cut(s) 491
MluCI AATT 3 cut(s) 312, 409, 429
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 4 cut(s) 45, 69, 130, 154
MroXI GAANNNNTTC 2 cut(s) 220, 373
MseI TTAA 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 285
MspI CCGG 2 cut(s) 14, 65
MunI CAATTG 1 cut(s) 409
NdeII GATC 2 cut(s) 361, 491
NlaIII CATG 1 cut(s) 362
NlaIV GGNNCC 2 cut(s) 12, 112
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 250
PdmI GAANNNNTTC 2 cut(s) 220, 373
PfeI GAWTC 2 cut(s) 80, 455
PkrI GCNGC 2 cut(s) 284, 287
PspN4I GGNNCC 2 cut(s) 12, 112
PstI CTGCAG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 491
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 2 cut(s) 283, 286
Sau3AI GATC 2 cut(s) 361, 491
SetI ASST 4 cut(s) 107, 221, 246, 332
SfaNI GCATC 1 cut(s) 20
SfcI CTRYAG 1 cut(s) 477
SpeI ACTAGT 1 cut(s) 254
Sse9I AATT 3 cut(s) 312, 409, 429
SsiI CCGC 3 cut(s) 186, 285, 324
SspMI CTAG 1 cut(s) 255
TaaI ACNGT 1 cut(s) 250
TaqI TCGA 3 cut(s) 28, 35, 379
TasI AATT 3 cut(s) 312, 409, 429
TauI GCSGC 1 cut(s) 288
TfiI GAWTC 2 cut(s) 80, 455
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 1 cut(s) 282
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 3 cut(s) 153, 321, 461
TspGWI ACGGA 1 cut(s) 476
XapI RAATTY 1 cut(s) 312
XmnI GAANNNNTTC 2 cut(s) 220, 373
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.