Rroxscaffold_7G00173220

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000243
Physical Location & Seq
Forward (+)
13310993 .. 13314504
3512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173220.1

Sequence Viewer

Length: 393 bp
ATGAATGAAATTCAATCTTTGCAGGGGACTGCATTCTGGATGGCCCCTGAGGTTTTTACTGCAAAAATCAAGAACCAAGGTTATGGACCTCCAGCGGATATATGGAGCCTTGGTTGCACTGTGTTGGAGATGTCAACAAGGCAGGTTCCCTACCCTGGTTTGGAACCGTTTCAGGCATGTTTTAAGATTGCAATGGGGGAGCTCCCTCACATTCCTCATTTTCATTGTAAGAAGGTGCGAGATTTCATCCATCAGTGCCTACAAGTTAATCCAAATAATCGACCCACTGCTACTCAGCTCTTATGTCATCCATTTGTCACGTCTGGGCCTATCAAACTACCCCATTTTGGAGATGGTCTTTGTATGCGGATGAAGGAAAAGAACTTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

130

Amino Acids

14.73

Weight (kDa)

8.59

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 106 5.9e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 10 - 101 1.3e-13 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 133
AciI CCGC 2 cut(s) 95, 367
AcsI RAATTY 1 cut(s) 9
AfiI CCNNNNNNNGG 3 cut(s) 155, 160, 347
AgsI TTSAA 1 cut(s) 14
AjiI CACGTC 1 cut(s) 321
AjnI CCWGG 1 cut(s) 154
AluBI AGCT 2 cut(s) 202, 298
AluI AGCT 2 cut(s) 202, 298
Alw21I GWGCWC 1 cut(s) 204
AoxI GGCC 2 cut(s) 42, 326
ApoI RAATTY 1 cut(s) 9
Asp700I GAANNNNTTC 1 cut(s) 168
AspS9I GGNCC 3 cut(s) 43, 86, 326
AvaII GGWCC 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 48
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 204
BccI CCATC 3 cut(s) 34, 258, 347
BciT130I CCWGG 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 156
Bme18I GGWCC 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 321
BmgT120I GGNCC 3 cut(s) 43, 86, 326
BmiI GGNNCC 4 cut(s) 45, 107, 147, 165
BmrFI CCNGG 1 cut(s) 156
BpmI CTGGAG 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 76, 109, 154
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 160, 347
Bse21I CCTNAGG 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 3 cut(s) 76, 109, 154
BseGI GGATG 4 cut(s) 45, 246, 307, 375
BseLI CCNNNNNNNGG 3 cut(s) 155, 160, 347
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 2 cut(s) 39, 308
BshFI GGCC 2 cut(s) 44, 328
BsiHKAI GWGCWC 1 cut(s) 204
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 3 cut(s) 155, 160, 347
BsmFI GGGAC 1 cut(s) 40
BsmI GAATGC 1 cut(s) 32
BsnI GGCC 2 cut(s) 44, 328
Bsp1286I GDGCHC 1 cut(s) 204
BspACI CCGC 2 cut(s) 95, 367
BspANI GGCC 2 cut(s) 44, 328
BspCNI CTCAG 2 cut(s) 40, 307
BspLI GGNNCC 4 cut(s) 45, 107, 147, 165
BspMI ACCTGC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 198
BssECI CCNNGG 3 cut(s) 76, 109, 154
BssT1I CCWWGG 2 cut(s) 76, 109
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 2 cut(s) 121, 168
BstDEI CTNAG 2 cut(s) 48, 294
BstF5I GGATG 4 cut(s) 45, 246, 307, 375
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 1 cut(s) 156
BstNSI RCATGY 1 cut(s) 180
BstSCI CCNGG 1 cut(s) 154
BstXI CCANNNNNNTGG 1 cut(s) 83
Bsu36I CCTNAGG 1 cut(s) 48
BsuRI GGCC 2 cut(s) 44, 328
BtrI CACGTC 1 cut(s) 321
BtsCI GGATG 4 cut(s) 45, 246, 307, 375
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 3 cut(s) 117, 260, 285
BveI ACCTGC 1 cut(s) 133
Cfr13I GGNCC 3 cut(s) 43, 86, 326
CviAII CATG 1 cut(s) 177
CviJI RGCY 5 cut(s) 44, 108, 202, 298, 328
CviKI_1 RGCY 5 cut(s) 44, 108, 202, 298, 328
DdeI CTNAG 2 cut(s) 48, 294
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 76, 109
Eco24I GRGCYC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 202
Eco81I CCTNAGG 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 154
EcoT14I CCWWGG 2 cut(s) 76, 109
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 2 cut(s) 76, 109
FaeI CATG 1 cut(s) 180
FaiI YATR 7 cut(s) 84, 101, 103, 178, 304, 365, 389
FaqI GGGAC 1 cut(s) 40
FatI CATG 1 cut(s) 176
FokI GGATG 4 cut(s) 52, 233, 294, 382
FriOI GRGCYC 1 cut(s) 204
GsuI CTGGAG 1 cut(s) 75
HaeIII GGCC 2 cut(s) 44, 328
Hin1II CATG 1 cut(s) 180
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
Hpy166II GTNNAC 1 cut(s) 135
Hpy188III TCNNGA 2 cut(s) 37, 70
Hpy8I GTNNAC 1 cut(s) 135
HpyAV CCTTC 2 cut(s) 226, 367
HpyCH4III ACNGT 2 cut(s) 121, 168
HpyCH4IV ACGT 1 cut(s) 320
HpyCH4V TGCA 5 cut(s) 22, 32, 62, 117, 191
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 2 cut(s) 48, 294
HpySE526I ACGT 1 cut(s) 320
Hsp92II CATG 1 cut(s) 180
LmnI GCTCC 3 cut(s) 105, 199, 207
LpnPI CCDG 9 cut(s) 8, 22, 60, 105, 128, 141, 158, 168, 309
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 1 cut(s) 9
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 4 cut(s) 43, 99, 216, 225
MroXI GAANNNNTTC 1 cut(s) 168
MseI TTAA 2 cut(s) 183, 267
MspA1I CMGCKG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 32
MvaI CCWGG 1 cut(s) 156
MwoI GCNNNNNNNGC 1 cut(s) 114
NlaIII CATG 1 cut(s) 180
NlaIV GGNNCC 4 cut(s) 45, 107, 147, 165
NmuCI GTSAC 1 cut(s) 316
NspI RCATGY 1 cut(s) 180
PctI GAATGC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 168
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspN4I GGNNCC 4 cut(s) 45, 107, 147, 165
PspPI GGNCC 3 cut(s) 43, 86, 326
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 2 cut(s) 183, 267
Sau96I GGNCC 3 cut(s) 43, 86, 326
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 1 cut(s) 204
SetI ASST 8 cut(s) 54, 82, 91, 147, 204, 237, 300, 323
SinI GGWCC 1 cut(s) 86
Sse9I AATT 1 cut(s) 9
SsiI CCGC 2 cut(s) 95, 367
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 2 cut(s) 76, 109
TaaI ACNGT 2 cut(s) 121, 168
TaiI ACGT 1 cut(s) 323
TaqI TCGA 1 cut(s) 280
TasI AATT 1 cut(s) 9
Tru1I TTAA 2 cut(s) 183, 267
Tru9I TTAA 2 cut(s) 183, 267
TscAI CASTG 3 cut(s) 124, 260, 292
TseFI GTSAC 1 cut(s) 316
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 5 cut(s) 17, 21, 212, 235, 386
TspRI CASTG 3 cut(s) 124, 260, 292
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 1 cut(s) 9
XceI RCATGY 1 cut(s) 180
XcmI CCANNNNNNNNNTGG 2 cut(s) 99, 350
XmnI GAANNNNTTC 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.