FvH4_3g44020

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000243
Physical Location & Seq
Reverse (-)
36723665 .. 36729791
6127 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44020.t1

Sequence Viewer

Length: 2154 bp
ATGTGGGATCTGAATGAGCCTCCTAAAGAGGACGATGACAACCCAAAAAGTGGCTGCAAAATATTCGGGGTCTGGCTCATGGACGGTTTTGATTCGCAACCCGTGACCCGACAGTTCTTCCCGGAAAACGTACCCTCCGAAATGGAACTCACGCCTGGAAGTGGTGGTGTTGCCACGTGGCCGGCTGCTTCTTCCTCTTCTTCATCATCATCACTTGTTCAGTGGAAGTATGACGTGTTCTTGAGCTTCAGGGGTCGAGACACTCGAAGAAGCATAACGTCTTATTTGTACTATCGACTGCAAAGGAGAGGTGTTAAGACATTCATGGATGATCCTGAACTTCAAGTAGGGGATGTGATTTCTCCCGCGTTGCAAAAGGCAATTGAAGAGTCAAGGTTTGCGATTGTTGTTCTGTCGGAAAACTTTGCTCATTCTAGTTGGTGTTTGGAGGAACTTGCAAAGATTTGTGAGTGTATGGAAGGAAGCAACAGAATATTTCCGGTTTTTTATAACGTGGAACCGTCTGATGTACGGCACCGAACCAAGAGCTTTGCAGAAGCTTTGAATAAGCATGAAAGCTACGGGCGACACACATTGGAGAATGTGCTGCGGTGGAATGATGCTTTAAGCAAAGTGTCCAATTTCTCTGGATGGCATACAAAGAATTATAAGACTGATAGAGAACTTGTGGATGCAATTGTGGATTTTGTGTGCAATAAAGTACAACCTATTGACACTGGGTTTACAATGTCTACCGAAGATTTTGAAGCATATGAAGCAACAAGGAAGGCCATGGATGAGGTTATGAAGGTGCTGAAAGATGAGGAGGTCACTGCGGTTGGTGTCTATGGCACGGGAGGAGTTGGCAAGACAACCAGGGTAAAACATGTCGCTGCACAATCCTGCAAAAGTGGGACTTCTCATCATGTGATTATGGCTGTTTTATCCCAGAGCCTTGACTTGACAAAAATTCAAGGCACATTGGCAGAGGCGAGCAGGTGTTGTACTGTGAATAGCAGTGGTAGTGCAAGTGTCGGAATTAAGGGAGTGCGGCCGCCAGGGCTGAAGCCGCCGCCTTCGATGGTGAGGGTTCCGGTGATGGACAATGGATGTTCGACTTGGGAGATTTTGAGGGGCTTCGGGCCGGAGGAAGAGAGGGTGTCGTTGGAAATGGTGCAGAGAAGGTTTTGTTCTTTTCCCTCTTCTTCTGACGAAGAAGAACTTGAAGAACAAGAAGTTGAACAAGGAGAGAATGTTGACTTTTCCAGCACTACCACAGAGTTTTCCAGTATTTCTCCATGTGGATGGTTTTTGCCAAATGGGAAGCCTATGCTTCCTATCACTTATTGGGAAAAGGGTGATTTACTCGGCAGCGGCTCTTTTGGGTGTGTGTATGAAGCAATTTCTGATGGCGGATGCTTCATCGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGGAGGCAAAGAGTTTCTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAGCATAGTTCAGTATTATGGCACACAAAAGGATGGATCAAAGCTATATATTTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAGAAGCTATATCACACGTACCATCTTACAGATACACATGTCTCTGTATACACGAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCGAAATGTGATTCACAGGGACATTAAATGTGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCTGACTTTGGATTGGCAAAGAGTATCAAACTGAACAACATAAAATCTTGCCAAGGAACTGCAAACTGGATGGCTCCCGAGGTTGTTAATCGTAAAAGTCATGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGAAGATTCCATACGATAATCTCGAACCGATGAAGGCATTATGGAGAATTGGAAAGGGAGAGCCCCCTCTGGTTCCTGATACTCTTTCAAGAGAGGCACAAGATTTCATCCATCTATGCTTAAAAGTTAAGCCAGATGATCGTCCCACTGCTGCTCAGCTTTTAGAACATCCGTTTGTAAATAAGCACTTTCCTCCCATGTCTTCGGGATCAGTATCTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

718

Amino Acids

79.93

Weight (kDa)

5.7

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 76 - 246 8.1e-47 TIR domain
TIR_2 PF13676 79 - 174 1.1e-15 TIR domain
Pkinase PF00069 451 - 703 9.3e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 453 - 699 1.4e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 510, 669
AarI CACCTGC 1 cut(s) 987
Acc36I ACCTGC 1 cut(s) 987
AccB1I GGYRCC 1 cut(s) 534
AccB7I CCANNNNNTGG 1 cut(s) 50
AccI GTMKAC 2 cut(s) 752, 1656
AccII CGCG 1 cut(s) 368
AciI CCGC 9 cut(s) 366, 610, 836, 1051, 1055, 1070, 1073, 1374, 1413
AclWI GGATC 5 cut(s) 15, 326, 1570, 1762, 2146
AcoI YGGCCR 2 cut(s) 179, 1052
AcsI RAATTY 1 cut(s) 969
AcuI CTGAAG 4 cut(s) 232, 1085, 1592, 1703
AcvI CACGTG 1 cut(s) 177
AfaI GTAC 7 cut(s) 132, 290, 531, 723, 1006, 1628, 1909
AfiI CCNNNNNNNGG 2 cut(s) 50, 161
AflIII ACRYGT 4 cut(s) 234, 886, 1623, 1645
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 4 cut(s) 154, 875, 1057, 1461
AluBI AGCT 9 cut(s) 246, 549, 560, 579, 1570, 1615, 1765, 1886, 2089
AluI AGCT 9 cut(s) 246, 549, 560, 579, 1570, 1615, 1765, 1886, 2089
Alw26I GTCTC 3 cut(s) 252, 1654, 1657
AlwI GGATC 5 cut(s) 15, 326, 1570, 1762, 2146
Ama87I CYCGRG 1 cut(s) 1844
AoxI GGCC 5 cut(s) 179, 789, 1052, 1142, 1876
ApeKI GCWGC 6 cut(s) 54, 185, 607, 893, 1371, 2081
ApoI RAATTY 1 cut(s) 969
AspS9I GGNCC 1 cut(s) 1142
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 3 cut(s) 1096, 1108, 1370
AvaI CYCGRG 1 cut(s) 1844
BaeI ACNNNNGTAYC 2 cut(s) 1632, 1665
BanI GGYRCC 1 cut(s) 534
BanII GRGCYC 1 cut(s) 1995
BarI GAAGNNNNNNTAC 2 cut(s) 1925, 1957
BauI CACGAG 1 cut(s) 1660
BbrPI CACGTG 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 2124
BbvI GCAGC 6 cut(s) 41, 172, 594, 880, 1383, 2068
BccI CCATC 9 cut(s) 645, 1075, 1093, 1299, 1403, 1553, 1638, 1831, 2049
BceAI ACGGC 1 cut(s) 548
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciT130I CCWGG 4 cut(s) 156, 877, 1059, 1463
BclI TGATCA 1 cut(s) 1450
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 3 cut(s) 252, 1654, 1657
BfaI CTAG 1 cut(s) 435
BfuAI ACCTGC 1 cut(s) 987
BglI GCCNNNNNGGC 1 cut(s) 1060
BglII AGATCT 1 cut(s) 1668
BlpI GCTNAGC 1 cut(s) 2085
Bme1390I CCNGG 5 cut(s) 122, 156, 877, 1059, 1463
BmeT110I CYCGRG 1 cut(s) 1844
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 1142
BmiI GGNNCC 6 cut(s) 519, 536, 1092, 1603, 1842, 2004
BmrFI CCNGG 5 cut(s) 122, 156, 877, 1059, 1463
BmrI ACTGGG 2 cut(s) 747, 1938
BmsI GCATC 3 cut(s) 610, 682, 1406
BmuI ACTGGG 2 cut(s) 747, 1938
BpiI GAAGAC 1 cut(s) 2124
Bpu1102I GCTNAGC 1 cut(s) 2085
BpuEI CTTGAG 1 cut(s) 262
BpuMI CCSGG 1 cut(s) 122
BsaAI YACGTR 2 cut(s) 177, 1626
BsaBI GATNNNNATC 1 cut(s) 2143
BsaJI CCNNGG 6 cut(s) 792, 876, 1058, 1462, 1819, 1845
BsaWI WCCGGW 2 cut(s) 499, 1093
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 1239, 1269
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 161
Bse118I RCCGGY 1 cut(s) 181
Bse1I ACTGG 4 cut(s) 742, 1287, 1838, 1933
Bse8I GATNNNNATC 1 cut(s) 2143
BseBI CCWGG 4 cut(s) 156, 877, 1059, 1463
BseDI CCNNGG 6 cut(s) 792, 876, 1058, 1462, 1819, 1845
BseJI GATNNNNATC 1 cut(s) 2143
BseLI CCNNNNNNNGG 2 cut(s) 50, 161
BseMII CTCAG 2 cut(s) 1499, 2099
BseNI ACTGG 4 cut(s) 742, 1287, 1838, 1933
BseRI GAGGAG 2 cut(s) 839, 873
BseX3I CGGCCG 1 cut(s) 1052
BseXI GCAGC 6 cut(s) 41, 172, 594, 880, 1383, 2068
BsgI GTGCAG 2 cut(s) 879, 1196
Bsh1236I CGCG 1 cut(s) 368
Bsh1285I CGRYCG 2 cut(s) 1055, 1699
BshFI GGCC 5 cut(s) 181, 791, 1054, 1144, 1878
BshNI GGYRCC 1 cut(s) 534
BsiEI CGRYCG 2 cut(s) 1055, 1699
BsiHKCI CYCGRG 1 cut(s) 1844
BsiSI CCGG 5 cut(s) 122, 182, 500, 1094, 1145
BslFI GGGAC 3 cut(s) 928, 1730, 2058
BslI CCNNNNNNNGG 2 cut(s) 50, 161
BsmAI GTCTC 3 cut(s) 252, 1654, 1657
BsmFI GGGAC 3 cut(s) 928, 1730, 2058
BsnI GGCC 5 cut(s) 181, 791, 1054, 1144, 1878
BsoBI CYCGRG 1 cut(s) 1844
Bsp1286I GDGCHC 1 cut(s) 1995
Bsp143I GATC 8 cut(s) 7, 331, 1450, 1562, 1668, 1754, 2068, 2138
Bsp1720I GCTNAGC 1 cut(s) 2085
Bsp19I CCATGG 1 cut(s) 792
BspACI CCGC 9 cut(s) 366, 610, 836, 1051, 1055, 1070, 1073, 1374, 1413
BspANI GGCC 5 cut(s) 181, 791, 1054, 1144, 1878
BspCNI CTCAG 2 cut(s) 1500, 2098
BspFNI CGCG 1 cut(s) 368
BspLI GGNNCC 6 cut(s) 519, 536, 1092, 1603, 1842, 2004
BspMI ACCTGC 1 cut(s) 987
BspPI GGATC 5 cut(s) 15, 326, 1570, 1762, 2146
BspQI GCTCTTC 1 cut(s) 1509
BspT107I GGYRCC 1 cut(s) 534
BsrFI RCCGGY 1 cut(s) 181
BsrI ACTGG 4 cut(s) 742, 1287, 1838, 1933
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 6 cut(s) 792, 876, 1058, 1462, 1819, 1845
BssMI GATC 8 cut(s) 7, 331, 1450, 1562, 1668, 1754, 2068, 2138
BssNAI GTATAC 1 cut(s) 1657
BssSI CACGAG 1 cut(s) 1660
BssT1I CCWWGG 2 cut(s) 792, 1819
Bst1107I GTATAC 1 cut(s) 1657
Bst2BI CACGAG 1 cut(s) 1660
Bst2UI CCWGG 4 cut(s) 156, 877, 1059, 1463
Bst4CI ACNGT 5 cut(s) 86, 114, 522, 1009, 1912
Bst6I CTCTTC 5 cut(s) 202, 381, 1146, 1207, 1509
BstBAI YACGTR 2 cut(s) 177, 1626
BstC8I GCNNGC 4 cut(s) 183, 994, 1746, 1767
BstDEI CTNAG 3 cut(s) 1508, 2085, 2151
BstDSI CCRYGG 1 cut(s) 792
BstFNI CGCG 1 cut(s) 368
BstKTI GATC 8 cut(s) 10, 334, 1453, 1565, 1671, 1757, 2071, 2141
BstMAI GTCTC 3 cut(s) 252, 1654, 1657
BstMBI GATC 8 cut(s) 7, 331, 1450, 1562, 1668, 1754, 2068, 2138
BstMCI CGRYCG 2 cut(s) 1055, 1699
BstMWI GCNNNNNNNGC 3 cut(s) 776, 1060, 1069
BstNI CCWGG 4 cut(s) 156, 877, 1059, 1463
BstNSI RCATGY 3 cut(s) 890, 1649, 1748
BstSCI CCNGG 5 cut(s) 120, 154, 875, 1057, 1461
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 6 cut(s) 41, 172, 594, 880, 1383, 2068
BstV2I GAAGAC 1 cut(s) 2124
BstX2I RGATCY 3 cut(s) 7, 1668, 1754
BstXI CCANNNNNNTGG 1 cut(s) 1305
BstYI RGATCY 3 cut(s) 7, 1668, 1754
BstZ17I GTATAC 1 cut(s) 1657
BstZI CGGCCG 1 cut(s) 1052
BsuRI GGCC 5 cut(s) 181, 791, 1054, 1144, 1878
BtgI CCRYGG 1 cut(s) 792
BtgZI GCGATG 1 cut(s) 1408
BtrI CACGTC 1 cut(s) 235
BtsI GCAGTG 3 cut(s) 831, 1024, 2076
BtsIMutI CAGTG 5 cut(s) 227, 735, 831, 1024, 2076
BveI ACCTGC 1 cut(s) 987
Cac8I GCNNGC 4 cut(s) 183, 994, 1746, 1767
CciNI GCGGCCGC 1 cut(s) 1052
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 1142
Csp6I GTAC 7 cut(s) 131, 289, 530, 722, 1005, 1627, 1908
CviQI GTAC 7 cut(s) 131, 289, 530, 722, 1005, 1627, 1908
DdeI CTNAG 3 cut(s) 1508, 2085, 2151
DpnI GATC 8 cut(s) 9, 333, 1452, 1564, 1670, 1756, 2070, 2140
DpnII GATC 8 cut(s) 7, 331, 1450, 1562, 1668, 1754, 2068, 2138
EaeI YGGCCR 2 cut(s) 179, 1052
EagI CGGCCG 1 cut(s) 1052
Eam1104I CTCTTC 5 cut(s) 202, 381, 1146, 1207, 1509
EarI CTCTTC 5 cut(s) 202, 381, 1146, 1207, 1509
EciI GGCGGA 1 cut(s) 1428
EclXI CGGCCG 1 cut(s) 1052
Eco130I CCWWGG 2 cut(s) 792, 1819
Eco24I GRGCYC 1 cut(s) 1995
Eco52I CGGCCG 1 cut(s) 1052
Eco57I CTGAAG 4 cut(s) 232, 1085, 1592, 1703
Eco72I CACGTG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 1844
EcoRII CCWGG 4 cut(s) 154, 875, 1057, 1461
EcoT14I CCWWGG 2 cut(s) 792, 1819
EcoT38I GRGCYC 1 cut(s) 1995
ErhI CCWWGG 2 cut(s) 792, 1819
FalI AAGNNNNNCTT 6 cut(s) 901, 933, 1206, 1238, 1590, 1622
FaqI GGGAC 3 cut(s) 928, 1730, 2058
FauI CCCGC 1 cut(s) 373
FauNDI CATATG 1 cut(s) 772
FbaI TGATCA 1 cut(s) 1450
FblI GTMKAC 2 cut(s) 752, 1656
FriOI GRGCYC 1 cut(s) 1995
FspBI CTAG 1 cut(s) 435
HaeIII GGCC 5 cut(s) 181, 791, 1054, 1144, 1878
HapII CCGG 5 cut(s) 122, 182, 500, 1094, 1145
HincII GTYRAC 2 cut(s) 1258, 1926
HindII GTYRAC 2 cut(s) 1258, 1926
HindIII AAGCTT 2 cut(s) 558, 1763
HinfI GANTC 6 cut(s) 92, 389, 1510, 1708, 1897, 1936
HpaI GTTAAC 1 cut(s) 1926
HpaII CCGG 5 cut(s) 122, 182, 500, 1094, 1145
HphI GGTGA 3 cut(s) 1096, 1108, 1370
Hpy166II GTNNAC 5 cut(s) 744, 753, 1258, 1657, 1926
Hpy8I GTNNAC 5 cut(s) 744, 753, 1258, 1657, 1926
HpyAV CCTTC 8 cut(s) 473, 781, 802, 1086, 1176, 1616, 1889, 1957
HpyCH4III ACNGT 5 cut(s) 86, 114, 522, 1009, 1912
HpyCH4IV ACGT 6 cut(s) 129, 176, 234, 278, 513, 1625
HpyF10VI GCNNNNNNNGC 3 cut(s) 776, 1060, 1069
HpyF3I CTNAG 3 cut(s) 1508, 2085, 2151
HpySE526I ACGT 6 cut(s) 129, 176, 234, 278, 513, 1625
KroI GCCGGC 1 cut(s) 181
KroNI GCCGGC 1 cut(s) 183
Ksp22I TGATCA 1 cut(s) 1450
KspAI GTTAAC 1 cut(s) 1926
Kzo9I GATC 8 cut(s) 7, 331, 1450, 1562, 1668, 1754, 2068, 2138
LguI GCTCTTC 1 cut(s) 1509
LmnI GCTCC 2 cut(s) 1607, 1846
Lsp1109I GCAGC 6 cut(s) 41, 172, 594, 880, 1383, 2068
LweI GCATC 3 cut(s) 610, 682, 1406
MaeI CTAG 1 cut(s) 435
MaeII ACGT 6 cut(s) 129, 176, 234, 278, 513, 1625
MaeIII GTNAC 3 cut(s) 103, 829, 1591
MalI GATC 8 cut(s) 9, 333, 1452, 1564, 1670, 1756, 2070, 2140
MboI GATC 8 cut(s) 7, 331, 1450, 1562, 1668, 1754, 2068, 2138
MfeI CAATTG 2 cut(s) 381, 696
MflI RGATCY 3 cut(s) 7, 1668, 1754
MhlI GDGCHC 1 cut(s) 1995
MluCI AATT 8 cut(s) 381, 640, 664, 696, 969, 1038, 1401, 1977
MlyI GAGTC 3 cut(s) 398, 1519, 1906
MmeI TCCRAC 4 cut(s) 396, 1015, 1146, 1896
MroNI GCCGGC 1 cut(s) 181
MseI TTAA 8 cut(s) 315, 626, 1041, 1722, 1854, 1925, 2051, 2058
MslI CAYNNNNRTG 3 cut(s) 1303, 1749, 1872
MspA1I CMGCKG 2 cut(s) 1374, 1886
MspI CCGG 5 cut(s) 122, 182, 500, 1094, 1145
MspR9I CCNGG 5 cut(s) 122, 156, 877, 1059, 1463
MunI CAATTG 2 cut(s) 381, 696
MvaI CCWGG 4 cut(s) 156, 877, 1059, 1463
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 3 cut(s) 776, 1060, 1069
NaeI GCCGGC 1 cut(s) 183
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 792
NdeI CATATG 1 cut(s) 772
NdeII GATC 8 cut(s) 7, 331, 1450, 1562, 1668, 1754, 2068, 2138
NgoMIV GCCGGC 1 cut(s) 181
NlaIV GGNNCC 6 cut(s) 519, 536, 1092, 1603, 1842, 2004
NmeAIII GCCGAG 1 cut(s) 1347
NmuCI GTSAC 2 cut(s) 103, 829
NotI GCGGCCGC 1 cut(s) 1052
NspI RCATGY 3 cut(s) 890, 1649, 1748
PaeI GCATGC 1 cut(s) 1748
PaqCI CACCTGC 1 cut(s) 987
PciI ACATGT 2 cut(s) 886, 1645
PciSI GCTCTTC 1 cut(s) 1509
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 262, 1950
PdiI GCCGGC 1 cut(s) 183
PfeI GAWTC 3 cut(s) 92, 1708, 1936
PflMI CCANNNNNTGG 1 cut(s) 50
PfoI TCCNGGA 1 cut(s) 120
PleI GAGTC 3 cut(s) 397, 1518, 1905
PmaCI CACGTG 1 cut(s) 177
PmlI CACGTG 1 cut(s) 177
PpsI GAGTC 3 cut(s) 397, 1518, 1905
Ppu21I YACGTR 2 cut(s) 177, 1626
PscI ACATGT 2 cut(s) 886, 1645
PsiI TTATAA 2 cut(s) 510, 669
Psp6I CCWGG 4 cut(s) 154, 875, 1057, 1461
PspCI CACGTG 1 cut(s) 177
PspGI CCWGG 4 cut(s) 154, 875, 1057, 1461
PspN4I GGNNCC 6 cut(s) 519, 536, 1092, 1603, 1842, 2004
PspPI GGNCC 1 cut(s) 1142
PsrI GAACNNNNNNTAC 2 cut(s) 221, 253
PsuI RGATCY 3 cut(s) 7, 1668, 1754
PvuII CAGCTG 1 cut(s) 1886
RsaI GTAC 7 cut(s) 132, 290, 531, 723, 1006, 1628, 1909
RsaNI GTAC 7 cut(s) 131, 289, 530, 722, 1005, 1627, 1908
RseI CAYNNNNRTG 3 cut(s) 1303, 1749, 1872
SapI GCTCTTC 1 cut(s) 1509
SaqAI TTAA 8 cut(s) 315, 626, 1041, 1722, 1854, 1925, 2051, 2058
Sau3AI GATC 8 cut(s) 7, 331, 1450, 1562, 1668, 1754, 2068, 2138
Sau96I GGNCC 1 cut(s) 1142
SchI GAGTC 3 cut(s) 398, 1519, 1906
ScrFI CCNGG 5 cut(s) 122, 156, 877, 1059, 1463
SduI GDGCHC 1 cut(s) 1995
SfaNI GCATC 3 cut(s) 610, 682, 1406
SmiMI CAYNNNNRTG 3 cut(s) 1303, 1749, 1872
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
SphI GCATGC 1 cut(s) 1748
Sse9I AATT 8 cut(s) 381, 640, 664, 696, 969, 1038, 1401, 1977
SsiI CCGC 9 cut(s) 366, 610, 836, 1051, 1055, 1070, 1073, 1374, 1413
SspI AATATT 2 cut(s) 63, 495
SspMI CTAG 1 cut(s) 435
StyD4I CCNGG 5 cut(s) 120, 154, 875, 1057, 1461
StyI CCWWGG 2 cut(s) 792, 1819
TaaI ACNGT 5 cut(s) 86, 114, 522, 1009, 1912
TaiI ACGT 6 cut(s) 132, 179, 237, 281, 516, 1628
TaqI TCGA 6 cut(s) 256, 265, 295, 1079, 1115, 1953
TaqII GACCGA 1 cut(s) 1713
TasI AATT 8 cut(s) 381, 640, 664, 696, 969, 1038, 1401, 1977
TatI WGTACW 4 cut(s) 288, 721, 1004, 1907
TauI GCSGC 5 cut(s) 1054, 1057, 1072, 1075, 1377
TfiI GAWTC 3 cut(s) 92, 1708, 1936
Tru1I TTAA 8 cut(s) 315, 626, 1041, 1722, 1854, 1925, 2051, 2058
Tru9I TTAA 8 cut(s) 315, 626, 1041, 1722, 1854, 1925, 2051, 2058
TscAI CASTG 5 cut(s) 227, 742, 838, 1024, 2083
TseFI GTSAC 2 cut(s) 103, 829
TseI GCWGC 6 cut(s) 54, 185, 607, 893, 1371, 2081
Tsp45I GTSAC 2 cut(s) 103, 829
TspDTI ATGAA 9 cut(s) 192, 313, 588, 789, 821, 1410, 1411, 1976, 2026
TspGWI ACGGA 1 cut(s) 2091
TspRI CASTG 5 cut(s) 227, 742, 838, 1024, 2083
Van91I CCANNNNNTGG 1 cut(s) 50
XapI RAATTY 1 cut(s) 969
XceI RCATGY 3 cut(s) 890, 1649, 1748
XcmI CCANNNNNNNNNTGG 1 cut(s) 1890
XmiI GTMKAC 2 cut(s) 752, 1656
XspI CTAG 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.