Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000243

Physical Location & Seq

Genomic Coordinates Reverse (-)

29318691 .. 29325088

Gene Structure 6398 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1227000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGGACGCCGCCAAGCAATCCAAACGTCCGAAACCCAAACTCGAACGCCGAAACGCCAGCAAGAACATCGACTACGACGCCTCTACCTTCTCCCCTTCCTCTTATTCTCCTTCCTACTCCTCCTCGCACGGCCGTCAGTCCCCTCTCTCCGACCGAACCAGCTTCCGTGTCAGTGGCATCGACGGTGAATTCGACCTCATCTGCCGCAGCCTTGGCCTTTCCGGCCCCGAAGACTTCGCCATCCCAATCGCAGCGTGGGAGGCCCAGCGGGCCCGCATGACCCCTCGCTCTCGCCTCCGCGATCAGAATCGTCATCCTCAGTTGCGAGACAGAGCAAACGTTGACGTTTTGGAGAAGAAATCCGAAGCTAGGGTTAGGCAGCTCGATAGTGAAATTAAGGCTTCGAGTTTGGCGGAATTTGGGGGAAATGTGAAAAATGGCGGTGCTGGTGGAGGAATTAAAGGTCCCCGCCCGCCTCCGGTTGTATTGAAATCATTTCCCCCCCTGGAGGACCACATTGGTGTAAATTCGCCGATTTGCGTTGGCGATATGAGCTTGGATGAAGAGGAGCTTGCAGTTAAAGCAAATGGCGAGAGGGAAGCGGTAAACGAAGCAAATGACGAGTGTAACTCATCTCCATCCAATGACAGTAATGATGATCAAAATGCAGATGACAGTGTTGGTTTTGTTGGCATTGTAAGATTGAACCCGGAGCCAATCCATAGTATTTTTTCTCCGAATGGGAAATTCAGGCGGAGTGTTTCGTCATCGTGGCAAAAGGGTCAGCTTTTGGGAAGTGGTTCTTATGGGACTGTGTATGAAGGCTTTACTGATGATGGATTCTTTTTTGCTGTAAAGGAGGTTTCTTTACTGGATCAGGGGAGCCAGGGGAAGCAGAGCATAATCCAACTTGAGCAGGAGATTCATCTTTTAAGTCAGTTTGAACATGACAACATAGTTCAATATCTGGGCACGGACAAGGACGAAACTAAGCTCTATATCTTCCTTGAGCTTGTAACAAAAGGCTCACTTGCAAATCTATATCAAAAGTATCTCTTGAGGGATTCTCAAGTCTCTGTGTTTACATGGCAGATTTTAAGCGGGTTGACATATCTTCACGATCGTAATGTGGTTCACAGGGATATCAAATGTGCTAATATATTGGTGGATGCAAGTGGATCTGTGAAACTTGCTGACTTTGGGTTAGCCAAGGCAACCAAATTTAATGACGTGAAGTCTTGCAAAGGGACTGCTTATTGGATGGCCCCTGAGGTTGTTAATCTAAGGAATCGTGGCTATGGGCTTGCAGCTGATATATGGAGCCTTGGATGCGCTGTGTTAGAGATGTTAACCCGCCAGCCTCCATACTCTCACCTGGAAGGCATGCAAGCTTTATTTCAGATTGGCCAGGGTGAACCTCCTCCAGTTCCTGATACCTTATCAAGAGATGCACGGGATTTCATTCTGAAATGTTTGCAAGTTAACCCTAATAGTCGACCTACTGCGGCTCAGCTATTGAACCATCCGTTTGTGAAAAAGCCACTCCACGCTTCCCCTGTCCCAGCTTCGCCTCATTGCAACAGTTTACGTTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000165 GO:0000186 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:1902531 GO:1902533

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

Pkinase

Protein Analysis

532

Amino Acids

58.44

Weight (kDa)

6.48

Isoelectric Point (pI)

48.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	258 - 511	3.8e-70	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	261 - 507	1.8e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca	FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica	MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica	Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis	pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis	RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata	RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora	Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii	Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa	Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha	Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana	Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1495
AccII	CGCG	1 cut(s)	300
AclI	AACGTT	1 cut(s)	339
AclWI	GGATC	2 cut(s)	882, 1186
AcoI	YGGCCR	2 cut(s)	130, 1405
AcsI	RAATTY	5 cut(s)	188, 416, 526, 746, 1220
AcyI	GRCGYC	2 cut(s)	6, 78
AfiI	CCNNNNNNNGG	3 cut(s)	369, 478, 508
AgsI	TTSAA	5 cut(s)	490, 706, 944, 962, 1519
AhdI	GACNNNNNGTC	1 cut(s)	1234
AjiI	CACGTC	1 cut(s)	1231
AjnI	CCWGG	4 cut(s)	504, 885, 1374, 1407
AleI	CACNNNNGTG	1 cut(s)	519
Alw26I	GTCTC	2 cut(s)	321, 1078
AlwI	GGATC	2 cut(s)	882, 1186
AlwNI	CAGNNNCTG	1 cut(s)	1430
AoxI	GGCC	7 cut(s)	130, 214, 223, 261, 270, 1263, 1405
ApaI	GGGCCC	1 cut(s)	274
ApeKI	GCWGC	4 cut(s)	207, 251, 379, 1307
ApoI	RAATTY	5 cut(s)	188, 416, 526, 746, 1220
ArsI	GACNNNNNNTTYG	2 cut(s)	173, 205
Asp700I	GAANNNNTTC	1 cut(s)	799
AspLEI	GCGC	1 cut(s)	1334
AspS9I	GGNCC	7 cut(s)	224, 262, 270, 271, 464, 511, 1264
AsuC2I	CCSGG	1 cut(s)	710
AsuHPI	GGTGA	3 cut(s)	197, 1364, 1424
AvaII	GGWCC	2 cut(s)	464, 511
AxyI	CCTNAGG	1 cut(s)	1269
BaeGI	GKGCMC	2 cut(s)	274, 974
BalI	TGGCCA	1 cut(s)	1407
BanII	GRGCYC	1 cut(s)	274
BbsI	GAAGAC	1 cut(s)	237
BbvI	GCAGC	4 cut(s)	219, 263, 391, 1319
BccI	CCATC	5 cut(s)	248, 646, 830, 1255, 1530
BceAI	ACGGC	2 cut(s)	117, 145
BcgI	CGANNNNNNTGC	2 cut(s)	49, 83
BciT130I	CCWGG	4 cut(s)	506, 887, 1376, 1409
BclI	TGATCA	1 cut(s)	658
BcnI	CCSGG	1 cut(s)	710
BcoDI	GTCTC	2 cut(s)	321, 1078
BfaI	CTAG	1 cut(s)	369
BglI	GCCNNNNNGGC	2 cut(s)	222, 269
BisI	GCNGC	7 cut(s)	9, 205, 208, 252, 380, 1308, 1506
BlpI	GCTNAGC	1 cut(s)	1509
BlsI	GCNGC	7 cut(s)	10, 206, 209, 253, 381, 1309, 1507
Bme1390I	CCNGG	5 cut(s)	506, 710, 887, 1376, 1409
Bme18I	GGWCC	2 cut(s)	464, 511
BmeRI	GACNNNNNGTC	1 cut(s)	1234
BmgBI	CACGTC	1 cut(s)	1231
BmgT120I	GGNCC	7 cut(s)	224, 262, 270, 271, 464, 511, 1264
BmiI	GGNNCC	7 cut(s)	226, 272, 466, 714, 884, 1266, 1322
BmrFI	CCNGG	5 cut(s)	506, 710, 887, 1376, 1409
BmsI	GCATC	4 cut(s)	186, 1159, 1319, 1438
BpiI	GAAGAC	1 cut(s)	237
BplI	GAGNNNNNCTC	4 cut(s)	614, 646, 1051, 1083
BpmI	CTGGAG	2 cut(s)	527, 1407
Bpu1102I	GCTNAGC	1 cut(s)	1509
BpuEI	CTTGAG	4 cut(s)	932, 1028, 1053, 1078
BpuMI	CCSGG	1 cut(s)	710
BsaBI	GATNNNNATC	1 cut(s)	306
BsaHI	GRCGYC	2 cut(s)	6, 78
BsaJI	CCNNGG	6 cut(s)	211, 504, 886, 1209, 1324, 1408
BsaWI	WCCGGW	1 cut(s)	478
Bsc4I	CCNNNNNNNGG	3 cut(s)	369, 478, 508
Bse1I	ACTGG	2 cut(s)	876, 1424
Bse21I	CCTNAGG	1 cut(s)	1269
Bse3DI	GCAATG	1 cut(s)	1573
Bse8I	GATNNNNATC	1 cut(s)	306
BseBI	CCWGG	4 cut(s)	506, 887, 1376, 1409
BseDI	CCNNGG	6 cut(s)	211, 504, 886, 1209, 1324, 1408
BseGI	GGATG	8 cut(s)	240, 313, 565, 638, 1174, 1266, 1334, 1522
BseJI	GATNNNNATC	1 cut(s)	306
BseLI	CCNNNNNNNGG	3 cut(s)	369, 478, 508
BseMI	GCAATG	1 cut(s)	1573
BseMII	CTCAG	3 cut(s)	332, 1260, 1523
BseNI	ACTGG	2 cut(s)	876, 1424
BseRI	GAGGAG	4 cut(s)	109, 112, 581, 1410
BseSI	GKGCMC	2 cut(s)	274, 974
BseX3I	CGGCCG	1 cut(s)	130
BseXI	GCAGC	4 cut(s)	219, 263, 391, 1319
BseYI	CCCAGC	2 cut(s)	264, 1561
Bsh1236I	CGCG	1 cut(s)	300
Bsh1285I	CGRYCG	3 cut(s)	133, 154, 1123
BshFI	GGCC	7 cut(s)	132, 216, 225, 263, 272, 1265, 1407
BsiEI	CGRYCG	3 cut(s)	133, 154, 1123
BsiSI	CCGG	3 cut(s)	222, 479, 710
BslFI	GGGAC	5 cut(s)	124, 450, 823, 1261, 1544
BslI	CCNNNNNNNGG	3 cut(s)	369, 478, 508
BsmAI	GTCTC	2 cut(s)	321, 1078
BsmFI	GGGAC	5 cut(s)	124, 450, 823, 1261, 1544
BsnI	GGCC	7 cut(s)	132, 216, 225, 263, 272, 1265, 1407
Bsp120I	GGGCCC	1 cut(s)	270
Bsp1286I	GDGCHC	2 cut(s)	274, 974
Bsp143I	GATC	5 cut(s)	301, 658, 874, 1120, 1178
Bsp1720I	GCTNAGC	1 cut(s)	1509
BspANI	GGCC	7 cut(s)	132, 216, 225, 263, 272, 1265, 1407
BspCNI	CTCAG	3 cut(s)	331, 1261, 1522
BspFNI	CGCG	1 cut(s)	300
BspLI	GGNNCC	7 cut(s)	226, 272, 466, 714, 884, 1266, 1322
BspPI	GGATC	2 cut(s)	882, 1186
BsrDI	GCAATG	1 cut(s)	1573
BsrI	ACTGG	2 cut(s)	876, 1424
BssECI	CCNNGG	6 cut(s)	211, 504, 886, 1209, 1324, 1408
BssMI	GATC	5 cut(s)	301, 658, 874, 1120, 1178
BssNI	GRCGYC	2 cut(s)	6, 78
BssT1I	CCWWGG	3 cut(s)	211, 1209, 1324
Bst2UI	CCWGG	4 cut(s)	506, 887, 1376, 1409
Bst4CI	ACNGT	5 cut(s)	185, 650, 677, 814, 1583
Bst6I	CTCTTC	1 cut(s)	558
BstACI	GRCGYC	2 cut(s)	6, 78
BstC8I	GCNNGC	9 cut(s)	58, 270, 274, 473, 573, 1305, 1358, 1385, 1389
BstDEI	CTNAG	5 cut(s)	318, 990, 1269, 1283, 1509
BstF5I	GGATG	8 cut(s)	240, 313, 565, 638, 1174, 1266, 1334, 1522
BstFNI	CGCG	1 cut(s)	300
BstHHI	GCGC	1 cut(s)	1334
BstKTI	GATC	5 cut(s)	304, 661, 877, 1123, 1181
BstMAI	GTCTC	2 cut(s)	321, 1078
BstMBI	GATC	5 cut(s)	301, 658, 874, 1120, 1178
BstMCI	CGRYCG	3 cut(s)	133, 154, 1123
BstMWI	GCNNNNNNNGC	7 cut(s)	213, 222, 260, 269, 552, 581, 1329
BstNI	CCWGG	4 cut(s)	506, 887, 1376, 1409
BstNSI	RCATGY	1 cut(s)	1387
BstSCI	CCNGG	5 cut(s)	504, 708, 885, 1374, 1407
BstSLI	GKGCMC	2 cut(s)	274, 974
BstUI	CGCG	1 cut(s)	300
BstV1I	GCAGC	4 cut(s)	219, 263, 391, 1319
BstV2I	GAAGAC	1 cut(s)	237
BstX2I	RGATCY	1 cut(s)	1178
BstYI	RGATCY	1 cut(s)	1178
BstZI	CGGCCG	1 cut(s)	130
Bsu36I	CCTNAGG	1 cut(s)	1269
BsuRI	GGCC	7 cut(s)	132, 216, 225, 263, 272, 1265, 1407
BtrI	CACGTC	1 cut(s)	1231
BtsCI	GGATG	8 cut(s)	240, 313, 565, 638, 1174, 1266, 1334, 1522
BtsIMutI	CAGTG	2 cut(s)	178, 682
Cac8I	GCNNGC	9 cut(s)	58, 270, 274, 473, 573, 1305, 1358, 1385, 1389
CaiI	CAGNNNCTG	1 cut(s)	1430
CfoI	GCGC	1 cut(s)	1334
Cfr13I	GGNCC	7 cut(s)	224, 262, 270, 271, 464, 511, 1264
CseI	GACGC	2 cut(s)	14, 86
CviAII	CATG	4 cut(s)	277, 947, 1086, 1384
DdeI	CTNAG	5 cut(s)	318, 990, 1269, 1283, 1509
DpnI	GATC	5 cut(s)	303, 660, 876, 1122, 1180
DpnII	GATC	5 cut(s)	301, 658, 874, 1120, 1178
DriI	GACNNNNNGTC	1 cut(s)	1234
EaeI	YGGCCR	2 cut(s)	130, 1405
EagI	CGGCCG	1 cut(s)	130
Eam1104I	CTCTTC	1 cut(s)	558
Eam1105I	GACNNNNNGTC	1 cut(s)	1234
EarI	CTCTTC	1 cut(s)	558
EciI	GGCGGA	2 cut(s)	428, 769
EclXI	CGGCCG	1 cut(s)	130
Eco130I	CCWWGG	3 cut(s)	211, 1209, 1324
Eco24I	GRGCYC	1 cut(s)	274
Eco32I	GATATC	1 cut(s)	1144
Eco47I	GGWCC	2 cut(s)	464, 511
Eco52I	CGGCCG	1 cut(s)	130
Eco81I	CCTNAGG	1 cut(s)	1269
EcoO109I	RGGNCCY	1 cut(s)	464
EcoRI	GAATTC	1 cut(s)	188
EcoRII	CCWGG	4 cut(s)	504, 885, 1374, 1407
EcoRV	GATATC	1 cut(s)	1144
EcoT14I	CCWWGG	3 cut(s)	211, 1209, 1324
EcoT38I	GRGCYC	1 cut(s)	274
ErhI	CCWWGG	3 cut(s)	211, 1209, 1324
FaeI	CATG	4 cut(s)	280, 950, 1089, 1387
FalI	AAGNNNNNCTT	8 cut(s)	555, 587, 787, 819, 1014, 1046, 1040, 1072
FaqI	GGGAC	5 cut(s)	124, 450, 823, 1261, 1544
FatI	CATG	4 cut(s)	276, 946, 1085, 1383
FauI	CCCGC	6 cut(s)	261, 281, 476, 480, 1094, 1361
FbaI	TGATCA	1 cut(s)	658
FblI	GTMKAC	1 cut(s)	1495
Fnu4HI	GCNGC	7 cut(s)	9, 205, 208, 252, 380, 1308, 1506
FokI	GGATG	8 cut(s)	227, 300, 572, 625, 1181, 1273, 1341, 1509
FriOI	GRGCYC	1 cut(s)	274
Fsp4HI	GCNGC	7 cut(s)	9, 205, 208, 252, 380, 1308, 1506
FspBI	CTAG	1 cut(s)	369
GlaI	GCGC	1 cut(s)	1333
GluI	GCNGC	7 cut(s)	9, 205, 208, 252, 380, 1308, 1506
GsaI	CCCAGC	2 cut(s)	268, 1565
GsuI	CTGGAG	2 cut(s)	527, 1407
HaeIII	GGCC	7 cut(s)	132, 216, 225, 263, 272, 1265, 1407
HapII	CCGG	3 cut(s)	222, 479, 710
HgaI	GACGC	2 cut(s)	14, 86
HhaI	GCGC	1 cut(s)	1334
Hin1I	GRCGYC	2 cut(s)	6, 78
Hin1II	CATG	4 cut(s)	280, 950, 1089, 1387
Hin6I	GCGC	1 cut(s)	1332
HinP1I	GCGC	1 cut(s)	1332
HincII	GTYRAC	5 cut(s)	343, 1107, 1350, 1483, 1496
HindII	GTYRAC	5 cut(s)	343, 1107, 1350, 1483, 1496
HindIII	AAGCTT	1 cut(s)	1389
HinfI	GANTC	5 cut(s)	307, 840, 922, 1064, 1288
HpaI	GTTAAC	2 cut(s)	1350, 1483
HpaII	CCGG	3 cut(s)	222, 479, 710
HphI	GGTGA	3 cut(s)	197, 1364, 1424
Hpy188I	TCNGA	7 cut(s)	30, 151, 306, 364, 738, 1401, 1467
Hpy188III	TCNNGA	5 cut(s)	1057, 1118, 1430, 1443, 1593
Hpy99I	CGWCG	2 cut(s)	80, 185
HpyAV	CCTTC	5 cut(s)	97, 105, 120, 815, 1373
HpyCH4III	ACNGT	5 cut(s)	185, 650, 677, 814, 1583
HpyCH4IV	ACGT	5 cut(s)	25, 339, 345, 1230, 1588
HpyF10VI	GCNNNNNNNGC	7 cut(s)	213, 222, 260, 269, 552, 581, 1329
HpyF3I	CTNAG	5 cut(s)	318, 990, 1269, 1283, 1509
HpySE526I	ACGT	5 cut(s)	25, 339, 345, 1230, 1588
Hsp92I	GRCGYC	2 cut(s)	6, 78
Hsp92II	CATG	4 cut(s)	280, 950, 1089, 1387
HspAI	GCGC	1 cut(s)	1332
Ksp22I	TGATCA	1 cut(s)	658
KspAI	GTTAAC	2 cut(s)	1350, 1483
Kzo9I	GATC	5 cut(s)	301, 658, 874, 1120, 1178
LmnI	GCTCC	4 cut(s)	568, 712, 882, 1320
Lsp1109I	GCAGC	4 cut(s)	219, 263, 391, 1319
LweI	GCATC	4 cut(s)	186, 1159, 1319, 1438
MaeI	CTAG	1 cut(s)	369
MaeII	ACGT	5 cut(s)	25, 339, 345, 1230, 1588
MaeIII	GTNAC	2 cut(s)	626, 1015
MalI	GATC	5 cut(s)	303, 660, 876, 1122, 1180
MboI	GATC	5 cut(s)	301, 658, 874, 1120, 1178
MboII	GAAGA	5 cut(s)	242, 367, 575, 994, 1106
MflI	RGATCY	1 cut(s)	1178
MhlI	GDGCHC	2 cut(s)	274, 974
MlsI	TGGCCA	1 cut(s)	1407
MluCI	AATT	7 cut(s)	188, 393, 416, 456, 526, 746, 1220
MluNI	TGGCCA	1 cut(s)	1407
MmeI	TCCRAC	2 cut(s)	174, 931
Mox20I	TGGCCA	1 cut(s)	1407
MroXI	GAANNNNTTC	1 cut(s)	799
MscI	TGGCCA	1 cut(s)	1407
MseI	TTAA	9 cut(s)	396, 459, 579, 932, 1097, 1224, 1278, 1349, 1482
MslI	CAYNNNNRTG	1 cut(s)	519
Msp20I	TGGCCA	1 cut(s)	1407
MspA1I	CMGCKG	2 cut(s)	268, 1310
MspI	CCGG	3 cut(s)	222, 479, 710
MspR9I	CCNGG	5 cut(s)	506, 710, 887, 1376, 1409
MvaI	CCWGG	4 cut(s)	506, 887, 1376, 1409
MvnI	CGCG	1 cut(s)	300
MwoI	GCNNNNNNNGC	7 cut(s)	213, 222, 260, 269, 552, 581, 1329
NciI	CCSGG	1 cut(s)	710
NdeII	GATC	5 cut(s)	301, 658, 874, 1120, 1178
NlaIII	CATG	4 cut(s)	280, 950, 1089, 1387
NlaIV	GGNNCC	7 cut(s)	226, 272, 466, 714, 884, 1266, 1322
NspI	RCATGY	1 cut(s)	1387
OliI	CACNNNNGTG	1 cut(s)	519
PaeI	GCATGC	1 cut(s)	1387
PcsI	WCGNNNNNNNCGW	1 cut(s)	189
PdmI	GAANNNNTTC	1 cut(s)	799
PfeI	GAWTC	5 cut(s)	307, 840, 922, 1064, 1288
PkrI	GCNGC	7 cut(s)	10, 206, 209, 253, 381, 1309, 1507
Ple19I	CGATCG	1 cut(s)	1123
PpuMI	RGGWCCY	1 cut(s)	464
Psp1406I	AACGTT	1 cut(s)	339
Psp5II	RGGWCCY	1 cut(s)	464
Psp6I	CCWGG	4 cut(s)	504, 885, 1374, 1407
PspFI	CCCAGC	2 cut(s)	264, 1561
PspGI	CCWGG	4 cut(s)	504, 885, 1374, 1407
PspN4I	GGNNCC	7 cut(s)	226, 272, 466, 714, 884, 1266, 1322
PspOMI	GGGCCC	1 cut(s)	270
PspPI	GGNCC	7 cut(s)	224, 262, 270, 271, 464, 511, 1264
PspPPI	RGGWCCY	1 cut(s)	464
PsrI	GAACNNNNNNTAC	2 cut(s)	56, 88
PstNI	CAGNNNCTG	1 cut(s)	1430
PsuI	RGATCY	1 cut(s)	1178
PvuI	CGATCG	1 cut(s)	1123
PvuII	CAGCTG	1 cut(s)	1310
RseI	CAYNNNNRTG	1 cut(s)	519
SalI	GTCGAC	1 cut(s)	1494
SaqAI	TTAA	9 cut(s)	396, 459, 579, 932, 1097, 1224, 1278, 1349, 1482
SatI	GCNGC	7 cut(s)	9, 205, 208, 252, 380, 1308, 1506
Sau3AI	GATC	5 cut(s)	301, 658, 874, 1120, 1178
Sau96I	GGNCC	7 cut(s)	224, 262, 270, 271, 464, 511, 1264
ScrFI	CCNGG	5 cut(s)	506, 710, 887, 1376, 1409
SduI	GDGCHC	2 cut(s)	274, 974
SfaNI	GCATC	4 cut(s)	186, 1159, 1319, 1438
SfiI	GGCCNNNNNGGCC	2 cut(s)	222, 269
SinI	GGWCC	2 cut(s)	464, 511
SmiMI	CAYNNNNRTG	1 cut(s)	519
SmlI	CTYRAG	4 cut(s)	911, 1007, 1057, 1068
SmoI	CTYRAG	4 cut(s)	911, 1007, 1057, 1068
SphI	GCATGC	1 cut(s)	1387
Sse9I	AATT	7 cut(s)	188, 393, 416, 456, 526, 746, 1220
SspMI	CTAG	1 cut(s)	369
StyD4I	CCNGG	5 cut(s)	504, 708, 885, 1374, 1407
StyI	CCWWGG	3 cut(s)	211, 1209, 1324
TaaI	ACNGT	5 cut(s)	185, 650, 677, 814, 1583
TaiI	ACGT	5 cut(s)	28, 342, 348, 1233, 1591
TaqI	TCGA	7 cut(s)	42, 69, 180, 192, 384, 404, 1495
TaqII	GACCGA	1 cut(s)	168
TasI	AATT	7 cut(s)	188, 393, 416, 456, 526, 746, 1220
TauI	GCSGC	3 cut(s)	11, 207, 1508
TfiI	GAWTC	5 cut(s)	307, 840, 922, 1064, 1288
Tru1I	TTAA	9 cut(s)	396, 459, 579, 932, 1097, 1224, 1278, 1349, 1482
Tru9I	TTAA	9 cut(s)	396, 459, 579, 932, 1097, 1224, 1278, 1349, 1482
TscAI	CASTG	2 cut(s)	178, 682
TseI	GCWGC	4 cut(s)	207, 251, 379, 1307
TspDTI	ATGAA	4 cut(s)	576, 834, 914, 1450
TspGWI	ACGGA	3 cut(s)	155, 989, 1515
TspRI	CASTG	2 cut(s)	178, 682
VpaK11BI	GGWCC	2 cut(s)	464, 511
XapI	RAATTY	5 cut(s)	188, 416, 526, 746, 1220
XceI	RCATGY	1 cut(s)	1387
XcmI	CCANNNNNNNNNTGG	1 cut(s)	252
XmiI	GTMKAC	1 cut(s)	1495
XmnI	GAANNNNTTC	1 cut(s)	799
XspI	CTAG	1 cut(s)	369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.