Prupe.1G505800_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000243
Physical Location & Seq
Reverse (-)
41777661 .. 41783817
6157 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G505800.1

Sequence Viewer

Length: 1713 bp
ATGCATCACATACCTCGAATTTTCTCTAGCAAGGGAGCGAAAACCATGGATCCGAAGAAGAACCGGAGGAAGCCGAGGCTGGAGCGCCGGAATGCGGTGAAGCACATTGACTACGACGCCGCTTCGACGTCGTCCTCGCTCGACGACTCGTCCTCTTCGCCTTCGCTCTACACGCGCTCCTTCGACTTGGACCGGACGAGCTTCCGGGTCGAGGGAAACGAGGGGGATATGGAGCGGATATACCAGACCTTGGGCCTTGGCCCCGACGACTTCTCCATATCAGAGGAGGACTGGGTGGCTCGGAAGATCCGGTCGTGTTCGGATCTGCTCCCCATGTCCAGATTGTACCAGTTGGATAGTCTGGATAGCCCCAAACCCGACGAATTGAGGGAAGTGGTGGCCGAGTTGTGCGATAGAGTTGGGGATGTTACTGTTACGGGGACTGAGTTGACTCGGGCTGACGCGGCCGGTCCAAGCGGGTTATGTAATGCTGCTACTGCTTCTACCACAGGCGGTGGTGTTTGTATTGGGATTAAGGGTGCACGGCCGCCGGTGCTTAAGCCTCCGCCGTCGATGAGAGTTCCGGTGGTCGACAATGGATGCTCAACTTGGGACCTTTTGAGGGACTTCGCGCCGGAAGGCGAGGGAGACTCGGTCCAGAAGAGGCTTGAGTCTTCTTCTTCTTCTGCTTCTTCTTGTTGTGGCGATGAAGAAGAAGGAGAGGTTGGAGAGGAGGGTGCTGTGGAGGCTGGGGTCAGAGAGACTGTGCCGCTCTCAGAAGGGTGTTCGTTTACGACGTCGAATGATGACGACTCTTCTAGCACTACTACTGTCTCTCCGAATGAGAGGTTTTCGCCCAATGGGAGGCTTAAGCTTAAAATCACTCACTGGGAAAAGGGTGACCTTCTGGGTAGTGGGTCTTTCGGGTCTGTGTATGAAGCAATTTCTGATTGCGGATGCTTCATTGCTGTGAAGGAAGTTTCCTTGCTGGATAAAGGAAGTCAGGGGGTGCAAAGAGTTTATCAACTTGAACAGGAGATTGCACTTTTGAGTCAGTTTGAACATCAAAACATAGTTCAATACTATGGAACCGCTAAGGATGAATCAAACCTTTATATCTTTCTGGAGCTTGTAGCCAAAGGCTCCCTCCAGAAACTCTATCAGACGTATCGTCTTACAGATTCTCATGTCTCTGAATACACAAGACAGATCCTGCAGGGTCTGAAGTATCTTCATGACCGAAGAGTGATTCACAGGGACATCAAATGCGCAAATATTTTGGTGCATGCAAATGGTTCTGTGAAGCTCGCAGATTTTGGATTGGCAAAGACTATCCAGATGAACGACATCAAATCTAGCCAGGGGACTGCATACTGGATGGCCCCTGAGGTTGTTAATCGGAAACACCAGGGATATGGGCTTCCGGCGGATATATGGAGCCTTGGATGCACTGTATTGGAGATGTTAACCAGACGGTTTCCATACCCTAATCTTGAATGGATGCAGGCATTATTTAAGATTGGAAAGGGAGAGCCCCCTCCGGTTCCCGATTCTCTTTCGAAAGATGCACAAGATTTCATCCATCGATGCCTACAAGTTAATCCAGCGAATCGTCCTACTGCTGCTCAGCTCTTAAATCATCCATTTCTTAATAGACCCCTTACGACATCTTCTGGATCAGGGTCTCCTTACCATCACCGTGGGCCGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

571

Amino Acids

62.7

Weight (kDa)

5.77

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 131, 800
Acc16I TGCGCA 1 cut(s) 1270
AccB7I CCANNNNNTGG 1 cut(s) 250
AccBSI CCGCTC 2 cut(s) 235, 772
AccI GTMKAC 1 cut(s) 591
AccII CGCG 3 cut(s) 175, 464, 632
AclWI GGATC 6 cut(s) 44, 57, 301, 330, 1204, 1684
AcoI YGGCCR 3 cut(s) 399, 465, 545
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 1244
AcyI GRCGYC 3 cut(s) 117, 128, 797
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 5 cut(s) 94, 211, 250, 622, 864
AflII CTTAAG 2 cut(s) 557, 869
AgsI TTSAA 4 cut(s) 1031, 1061, 1079, 1496
AhdI GACNNNNNGTC 2 cut(s) 148, 1170
AjnI CCWGG 2 cut(s) 1359, 1407
AjuI GAANNNNNNNTTGG 2 cut(s) 708, 740
AluBI AGCT 5 cut(s) 201, 874, 1129, 1306, 1630
AluI AGCT 5 cut(s) 201, 874, 1129, 1306, 1630
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 5 cut(s) 642, 755, 838, 1195, 1689
Alw44I GTGCAC 1 cut(s) 540
AlwI GGATC 6 cut(s) 44, 57, 301, 330, 1204, 1684
AlwNI CAGNNNCTG 2 cut(s) 1213, 1222
Ama87I CYCGRG 1 cut(s) 453
AoxI GGCC 7 cut(s) 253, 259, 399, 465, 545, 1380, 1703
ApaLI GTGCAC 1 cut(s) 540
ApeKI GCWGC 2 cut(s) 491, 1622
ApoI RAATTY 1 cut(s) 18
AspLEI GCGC 4 cut(s) 87, 177, 634, 1271
AspS9I GGNCC 8 cut(s) 190, 253, 260, 470, 613, 655, 1381, 1703
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 3 cut(s) 109, 911, 1688
AsuII TTCGAA 1 cut(s) 1559
AvaI CYCGRG 1 cut(s) 453
AvaII GGWCC 4 cut(s) 190, 470, 613, 655
AxyI CCTNAGG 1 cut(s) 1386
BaeGI GKGCMC 1 cut(s) 544
BamHI GGATCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 1536
BbsI GAAGAC 1 cut(s) 666
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 2 cut(s) 478, 1609
BccI CCATC 3 cut(s) 1372, 1590, 1701
BceAI ACGGC 2 cut(s) 553, 560
BciT130I CCWGG 2 cut(s) 1361, 1409
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 5 cut(s) 642, 755, 838, 1195, 1689
BfaI CTAG 3 cut(s) 27, 819, 1356
BfmI CTRYAG 1 cut(s) 1214
BfoI RGCGCY 1 cut(s) 88
BfrI CTTAAG 2 cut(s) 557, 869
BisI GCNGC 6 cut(s) 120, 465, 492, 548, 770, 1623
BlpI GCTNAGC 1 cut(s) 1626
BlsI GCNGC 6 cut(s) 121, 466, 493, 549, 771, 1624
Bme1390I CCNGG 3 cut(s) 206, 1361, 1409
Bme18I GGWCC 4 cut(s) 190, 470, 613, 655
BmeRI GACNNNNNGTC 2 cut(s) 148, 1170
BmeT110I CYCGRG 1 cut(s) 453
BmgT120I GGNCC 8 cut(s) 190, 253, 260, 470, 613, 655, 1381, 1703
BmiI GGNNCC 8 cut(s) 51, 262, 614, 1090, 1144, 1383, 1439, 1545
BmrFI CCNGG 3 cut(s) 206, 1361, 1409
BmrI ACTGGG 2 cut(s) 301, 898
BmsI GCATC 7 cut(s) 13, 590, 947, 1436, 1491, 1555, 1577
BmuI ACTGGG 2 cut(s) 301, 898
BpiI GAAGAC 1 cut(s) 666
BplI GAGNNNNNCTC 2 cut(s) 635, 667
BpmI CTGGAG 3 cut(s) 101, 1133, 1145
Bpu10I CCTNAGC 1 cut(s) 1095
Bpu1102I GCTNAGC 1 cut(s) 1626
Bpu14I TTCGAA 1 cut(s) 1559
BpuEI CTTGAG 1 cut(s) 689
BpuMI CCSGG 1 cut(s) 206
Bsa29I ATCGAT 1 cut(s) 1585
BsaHI GRCGYC 3 cut(s) 117, 128, 797
BsaI GGTCTC 1 cut(s) 1689
BsaJI CCNNGG 8 cut(s) 45, 74, 249, 256, 1360, 1408, 1441, 1699
BsaWI WCCGGW 5 cut(s) 63, 192, 309, 583, 1540
BsaXI ACNNNNNCTCC 8 cut(s) 161, 191, 257, 287, 639, 669, 820, 850
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 211, 250, 622, 864
Bse118I RCCGGY 2 cut(s) 467, 550
Bse1I ACTGG 4 cut(s) 296, 349, 893, 1379
Bse21I CCTNAGG 1 cut(s) 1386
Bse3DI GCAATG 1 cut(s) 963
BseBI CCWGG 2 cut(s) 1361, 1409
BseCI ATCGAT 1 cut(s) 1585
BseDI CCNNGG 8 cut(s) 45, 74, 249, 256, 1360, 1408, 1441, 1699
BseGI GGATG 9 cut(s) 430, 605, 962, 1105, 1383, 1451, 1506, 1578, 1639
BseLI CCNNNNNNNGG 5 cut(s) 94, 211, 250, 622, 864
BseMI GCAATG 1 cut(s) 963
BseMII CTCAG 4 cut(s) 435, 789, 1377, 1640
BseNI ACTGG 4 cut(s) 296, 349, 893, 1379
BseRI GAGGAG 2 cut(s) 299, 746
BseSI GKGCMC 1 cut(s) 544
BseX3I CGGCCG 2 cut(s) 465, 545
BseXI GCAGC 2 cut(s) 478, 1609
BseYI CCCAGC 1 cut(s) 749
Bsh1236I CGCG 3 cut(s) 175, 464, 632
Bsh1285I CGRYCG 3 cut(s) 314, 468, 548
BshFI GGCC 7 cut(s) 255, 261, 401, 467, 547, 1382, 1705
BshVI ATCGAT 1 cut(s) 1585
BsiEI CGRYCG 3 cut(s) 314, 468, 548
BsiHKAI GWGCWC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 453
BslFI GGGAC 5 cut(s) 454, 626, 638, 1271, 1378
BslI CCNNNNNNNGG 5 cut(s) 94, 211, 250, 622, 864
BsmAI GTCTC 5 cut(s) 642, 755, 838, 1195, 1689
BsmFI GGGAC 5 cut(s) 454, 626, 638, 1271, 1378
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 7 cut(s) 255, 261, 401, 467, 547, 1382, 1705
Bso31I GGTCTC 1 cut(s) 1689
BsoBI CYCGRG 1 cut(s) 453
Bsp119I TTCGAA 1 cut(s) 1559
Bsp1286I GDGCHC 2 cut(s) 544, 1536
Bsp143I GATC 5 cut(s) 49, 306, 322, 1209, 1676
Bsp1720I GCTNAGC 1 cut(s) 1626
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 7 cut(s) 255, 261, 401, 467, 547, 1382, 1705
BspCNI CTCAG 4 cut(s) 436, 788, 1378, 1639
BspDI ATCGAT 1 cut(s) 1585
BspFNI CGCG 3 cut(s) 175, 464, 632
BspHI TCATGA 1 cut(s) 1234
BspLI GGNNCC 8 cut(s) 51, 262, 614, 1090, 1144, 1383, 1439, 1545
BspMAI CTGCAG 1 cut(s) 1218
BspPI GGATC 6 cut(s) 44, 57, 301, 330, 1204, 1684
BspT104I TTCGAA 1 cut(s) 1559
BspTI CTTAAG 2 cut(s) 557, 869
BspTNI GGTCTC 1 cut(s) 1689
BsrBI CCGCTC 2 cut(s) 235, 772
BsrDI GCAATG 1 cut(s) 963
BsrFI RCCGGY 2 cut(s) 467, 550
BsrI ACTGG 4 cut(s) 296, 349, 893, 1379
BssAI RCCGGY 2 cut(s) 467, 550
BssECI CCNNGG 8 cut(s) 45, 74, 249, 256, 1360, 1408, 1441, 1699
BssMI GATC 5 cut(s) 49, 306, 322, 1209, 1676
BssNI GRCGYC 3 cut(s) 117, 128, 797
BssT1I CCWWGG 4 cut(s) 45, 249, 256, 1441
Bst2UI CCWGG 2 cut(s) 1361, 1409
Bst4CI ACNGT 6 cut(s) 433, 766, 832, 1453, 1476, 1700
Bst6I CTCTTC 4 cut(s) 160, 656, 820, 1237
BstACI GRCGYC 3 cut(s) 117, 128, 797
BstAFI CTTAAG 2 cut(s) 557, 869
BstBI TTCGAA 1 cut(s) 1559
BstC8I GCNNGC 3 cut(s) 1287, 1308, 1506
BstDEI CTNAG 5 cut(s) 444, 775, 1095, 1386, 1626
BstDSI CCRYGG 2 cut(s) 45, 1699
BstEII GGTNACC 1 cut(s) 899
BstF5I GGATG 9 cut(s) 430, 605, 962, 1105, 1383, 1451, 1506, 1578, 1639
BstFNI CGCG 3 cut(s) 175, 464, 632
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 4 cut(s) 87, 177, 634, 1271
BstKTI GATC 5 cut(s) 52, 309, 325, 1212, 1679
BstMAI GTCTC 5 cut(s) 642, 755, 838, 1195, 1689
BstMBI GATC 5 cut(s) 49, 306, 322, 1209, 1676
BstMCI CGRYCG 3 cut(s) 314, 468, 548
BstMWI GCNNNNNNNGC 6 cut(s) 172, 464, 497, 553, 746, 1446
BstNI CCWGG 2 cut(s) 1361, 1409
BstNSI RCATGY 1 cut(s) 1289
BstPI GGTNACC 1 cut(s) 899
BstSCI CCNGG 3 cut(s) 204, 1359, 1407
BstSFI CTRYAG 1 cut(s) 1214
BstSLI GKGCMC 1 cut(s) 544
BstUI CGCG 3 cut(s) 175, 464, 632
BstV1I GCAGC 2 cut(s) 478, 1609
BstV2I GAAGAC 1 cut(s) 666
BstX2I RGATCY 4 cut(s) 49, 306, 322, 1209
BstXI CCANNNNNNTGG 2 cut(s) 1415, 1700
BstYI RGATCY 4 cut(s) 49, 306, 322, 1209
BstZI CGGCCG 2 cut(s) 465, 545
Bsu15I ATCGAT 1 cut(s) 1585
Bsu36I CCTNAGG 1 cut(s) 1386
BsuRI GGCC 7 cut(s) 255, 261, 401, 467, 547, 1382, 1705
BsuTUI ATCGAT 1 cut(s) 1585
BtgI CCRYGG 2 cut(s) 45, 1699
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 9 cut(s) 430, 605, 962, 1105, 1383, 1451, 1506, 1578, 1639
BtsIMutI CAGTG 2 cut(s) 886, 1449
Cac8I GCNNGC 3 cut(s) 1287, 1308, 1506
CaiI CAGNNNCTG 2 cut(s) 1213, 1222
CciI TCATGA 1 cut(s) 1234
CfoI GCGC 4 cut(s) 87, 177, 634, 1271
Cfr10I RCCGGY 2 cut(s) 467, 550
Cfr13I GGNCC 8 cut(s) 190, 253, 260, 470, 613, 655, 1381, 1703
ClaI ATCGAT 1 cut(s) 1585
CseI GACGC 2 cut(s) 125, 470
Csp6I GTAC 1 cut(s) 346
CviAII CATG 5 cut(s) 46, 334, 1187, 1235, 1286
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 5 cut(s) 444, 775, 1095, 1386, 1626
DpnI GATC 5 cut(s) 51, 308, 324, 1211, 1678
DpnII GATC 5 cut(s) 49, 306, 322, 1209, 1676
DriI GACNNNNNGTC 2 cut(s) 148, 1170
EaeI YGGCCR 3 cut(s) 399, 465, 545
EagI CGGCCG 2 cut(s) 465, 545
Eam1104I CTCTTC 4 cut(s) 160, 656, 820, 1237
Eam1105I GACNNNNNGTC 2 cut(s) 148, 1170
EarI CTCTTC 4 cut(s) 160, 656, 820, 1237
EciI GGCGGA 2 cut(s) 555, 1442
EclXI CGGCCG 2 cut(s) 465, 545
Eco130I CCWWGG 4 cut(s) 45, 249, 256, 1441
Eco24I GRGCYC 1 cut(s) 1536
Eco31I GGTCTC 1 cut(s) 1689
Eco47I GGWCC 4 cut(s) 190, 470, 613, 655
Eco52I CGGCCG 2 cut(s) 465, 545
Eco57I CTGAAG 1 cut(s) 1244
Eco81I CCTNAGG 1 cut(s) 1386
Eco88I CYCGRG 1 cut(s) 453
Eco91I GGTNACC 1 cut(s) 899
EcoO109I RGGNCCY 1 cut(s) 613
EcoO65I GGTNACC 1 cut(s) 899
EcoRII CCWGG 2 cut(s) 1359, 1407
EcoT14I CCWWGG 4 cut(s) 45, 249, 256, 1441
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1536
ErhI CCWWGG 4 cut(s) 45, 249, 256, 1441
FaeI CATG 5 cut(s) 49, 337, 1190, 1238, 1289
FaqI GGGAC 5 cut(s) 454, 626, 638, 1271, 1378
FatI CATG 5 cut(s) 45, 333, 1186, 1234, 1285
FauI CCCGC 1 cut(s) 470
FblI GTMKAC 1 cut(s) 591
Fnu4HI GCNGC 6 cut(s) 120, 465, 492, 548, 770, 1623
FokI GGATG 9 cut(s) 437, 612, 969, 1112, 1390, 1458, 1513, 1565, 1626
FriOI GRGCYC 1 cut(s) 1536
Fsp4HI GCNGC 6 cut(s) 120, 465, 492, 548, 770, 1623
FspBI CTAG 3 cut(s) 27, 819, 1356
FspI TGCGCA 1 cut(s) 1270
GlaI GCGC 4 cut(s) 86, 176, 633, 1270
GluI GCNGC 6 cut(s) 120, 465, 492, 548, 770, 1623
GsaI CCCAGC 1 cut(s) 753
GsuI CTGGAG 3 cut(s) 101, 1133, 1145
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 7 cut(s) 255, 261, 401, 467, 547, 1382, 1705
HgaI GACGC 2 cut(s) 125, 470
HhaI GCGC 4 cut(s) 87, 177, 634, 1271
Hin1I GRCGYC 3 cut(s) 117, 128, 797
Hin1II CATG 5 cut(s) 49, 337, 1190, 1238, 1289
Hin6I GCGC 4 cut(s) 85, 175, 632, 1269
HinP1I GCGC 4 cut(s) 85, 175, 632, 1269
HincII GTYRAC 3 cut(s) 450, 592, 1467
HindII GTYRAC 3 cut(s) 450, 592, 1467
HindIII AAGCTT 1 cut(s) 872
HpaI GTTAAC 1 cut(s) 1467
HphI GGTGA 3 cut(s) 109, 911, 1688
Hpy166II GTNNAC 5 cut(s) 450, 542, 592, 792, 1467
Hpy8I GTNNAC 5 cut(s) 450, 542, 592, 792, 1467
Hpy99I CGWCG 9 cut(s) 119, 130, 133, 146, 269, 383, 574, 799, 802
HpyAV CCTTC 7 cut(s) 171, 190, 632, 710, 773, 914, 967
HpyCH4III ACNGT 6 cut(s) 433, 766, 832, 1453, 1476, 1700
HpyCH4IV ACGT 3 cut(s) 128, 797, 1166
HpyF10VI GCNNNNNNNGC 6 cut(s) 172, 464, 497, 553, 746, 1446
HpyF3I CTNAG 5 cut(s) 444, 775, 1095, 1386, 1626
HpySE526I ACGT 3 cut(s) 128, 797, 1166
Hsp92I GRCGYC 3 cut(s) 117, 128, 797
Hsp92II CATG 5 cut(s) 49, 337, 1190, 1238, 1289
HspAI GCGC 4 cut(s) 85, 175, 632, 1269
KspAI GTTAAC 1 cut(s) 1467
Kzo9I GATC 5 cut(s) 49, 306, 322, 1209, 1676
LmnI GCTCC 8 cut(s) 35, 82, 182, 232, 333, 1126, 1148, 1437
Lsp1109I GCAGC 2 cut(s) 478, 1609
LweI GCATC 7 cut(s) 13, 590, 947, 1436, 1491, 1555, 1577
MaeI CTAG 3 cut(s) 27, 819, 1356
MaeII ACGT 3 cut(s) 128, 797, 1166
MaeIII GTNAC 3 cut(s) 427, 433, 899
MalI GATC 5 cut(s) 51, 308, 324, 1211, 1678
MbiI CCGCTC 2 cut(s) 235, 772
MboI GATC 5 cut(s) 49, 306, 322, 1209, 1676
MflI RGATCY 4 cut(s) 49, 306, 322, 1209
MhlI GDGCHC 2 cut(s) 544, 1536
MluCI AATT 3 cut(s) 18, 383, 942
MlyI GAGTC 6 cut(s) 140, 445, 644, 680, 806, 1060
MmeI TCCRAC 2 cut(s) 333, 706
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 3 cut(s) 968, 1290, 1698
MspCI CTTAAG 2 cut(s) 557, 869
MspR9I CCNGG 3 cut(s) 206, 1361, 1409
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 2 cut(s) 1361, 1409
MvnI CGCG 3 cut(s) 175, 464, 632
MwoI GCNNNNNNNGC 6 cut(s) 172, 464, 497, 553, 746, 1446
NciI CCSGG 1 cut(s) 206
NcoI CCATGG 1 cut(s) 45
NdeII GATC 5 cut(s) 49, 306, 322, 1209, 1676
NlaIII CATG 5 cut(s) 49, 337, 1190, 1238, 1289
NlaIV GGNNCC 8 cut(s) 51, 262, 614, 1090, 1144, 1383, 1439, 1545
NmeAIII GCCGAG 2 cut(s) 99, 427
NmuCI GTSAC 1 cut(s) 899
NsbI TGCGCA 1 cut(s) 1270
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 1289
NspV TTCGAA 1 cut(s) 1559
PaeI GCATGC 1 cut(s) 1289
PagI TCATGA 1 cut(s) 1234
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 794
PctI GAATGC 1 cut(s) 97
PfeI GAWTC 5 cut(s) 1103, 1181, 1249, 1550, 1609
PflFI GACNNNGTC 2 cut(s) 130, 653
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 6 cut(s) 121, 466, 493, 549, 771, 1624
PleI GAGTC 6 cut(s) 140, 445, 644, 679, 806, 1059
PpsI GAGTC 6 cut(s) 140, 445, 644, 679, 806, 1059
PpuMI RGGWCCY 1 cut(s) 613
Psp5II RGGWCCY 1 cut(s) 613
Psp6I CCWGG 2 cut(s) 1359, 1407
PspEI GGTNACC 1 cut(s) 899
PspFI CCCAGC 1 cut(s) 749
PspGI CCWGG 2 cut(s) 1359, 1407
PspN4I GGNNCC 8 cut(s) 51, 262, 614, 1090, 1144, 1383, 1439, 1545
PspPI GGNCC 8 cut(s) 190, 253, 260, 470, 613, 655, 1381, 1703
PspPPI RGGWCCY 1 cut(s) 613
PstI CTGCAG 1 cut(s) 1218
PstNI CAGNNNCTG 2 cut(s) 1213, 1222
PsuI RGATCY 4 cut(s) 49, 306, 322, 1209
PsyI GACNNNGTC 2 cut(s) 130, 653
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
RseI CAYNNNNRTG 3 cut(s) 968, 1290, 1698
SalI GTCGAC 1 cut(s) 590
SatI GCNGC 6 cut(s) 120, 465, 492, 548, 770, 1623
Sau3AI GATC 5 cut(s) 49, 306, 322, 1209, 1676
Sau96I GGNCC 8 cut(s) 190, 253, 260, 470, 613, 655, 1381, 1703
SbfI CCTGCAGG 1 cut(s) 1218
SchI GAGTC 6 cut(s) 140, 445, 644, 680, 806, 1060
ScrFI CCNGG 3 cut(s) 206, 1361, 1409
SdaI CCTGCAGG 1 cut(s) 1218
SduI GDGCHC 2 cut(s) 544, 1536
SfaNI GCATC 7 cut(s) 13, 590, 947, 1436, 1491, 1555, 1577
SfcI CTRYAG 1 cut(s) 1214
SfuI TTCGAA 1 cut(s) 1559
SgrAI CRCCGGYG 1 cut(s) 550
SinI GGWCC 4 cut(s) 190, 470, 613, 655
SmiMI CAYNNNNRTG 3 cut(s) 968, 1290, 1698
SmlI CTYRAG 3 cut(s) 557, 668, 869
SmoI CTYRAG 3 cut(s) 557, 668, 869
SphI GCATGC 1 cut(s) 1289
Sse8387I CCTGCAGG 1 cut(s) 1218
Sse9I AATT 3 cut(s) 18, 383, 942
SspI AATATT 1 cut(s) 1276
SspMI CTAG 3 cut(s) 27, 819, 1356
StyD4I CCNGG 3 cut(s) 204, 1359, 1407
StyI CCWWGG 4 cut(s) 45, 249, 256, 1441
TaaI ACNGT 6 cut(s) 433, 766, 832, 1453, 1476, 1700
TaiI ACGT 3 cut(s) 131, 800, 1169
TaqII GACCGA 2 cut(s) 643, 1254
TasI AATT 3 cut(s) 18, 383, 942
TauI GCSGC 4 cut(s) 122, 467, 550, 772
TfiI GAWTC 5 cut(s) 1103, 1181, 1249, 1550, 1609
TscAI CASTG 2 cut(s) 893, 1456
TseFI GTSAC 1 cut(s) 899
TseI GCWGC 2 cut(s) 491, 1622
Tsp45I GTSAC 1 cut(s) 899
TspDTI ATGAA 7 cut(s) 723, 951, 952, 1116, 1223, 1355, 1567
TspRI CASTG 2 cut(s) 893, 1456
Tth111I GACNNNGTC 2 cut(s) 130, 653
Van91I CCANNNNNTGG 1 cut(s) 250
Vha464I CTTAAG 2 cut(s) 557, 869
VneI GTGCAC 1 cut(s) 540
VpaK11BI GGWCC 4 cut(s) 190, 470, 613, 655
XapI RAATTY 1 cut(s) 18
XceI RCATGY 1 cut(s) 1289
XmiI GTMKAC 1 cut(s) 591
XspI CTAG 3 cut(s) 27, 819, 1356
ZraI GACGTC 2 cut(s) 129, 798
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.