RLG00000000612

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000243
Physical Location & Seq
Reverse (-)
2587915 .. 2593270
5356 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000612

Sequence Viewer

Length: 1914 bp
ATGGATCCGAAGAAGGCCCGGCTGTTGCCAAAGTTGGAGCGCCGCAATGCGGTCAAGCACATTGACTACGACGCCTCTTCGTCCTCCTCCTCCCGCGACAACTCCACCCACGCGCAGCAGCTCCACACGCGCTCGCTCGACCTGTCGGACCGGACCAGCTTCCGGGTAGAAGGAAATGACGGCGAGTTCGAACGGATTTGCGCCGAGCTGGGCTTCACCGGCCCAGACGACTTCGCAATACCCGAGGCGGCCTGGGAGTCCCGGAAGATCCGGTCCACCAACTCGGATGTTCTCCCCCTGTCAAAACTGTACGTGATGGATAGTCCGAGACCCGACCCGAAGGACGAATCGGAGCATGAGGATGATGCCGTGGCGGAATTGAGCAATAGAGTTAGGGATAGCGTTACTGTTACGGTGGCCGAGTCGACTCGGTCCGAGTCCGAGTCGGCTGGGCCGAGCGGGTGCTGTACTGCGAGTAGTAGTTGTAGTGGTAGTGTCGGAATTAAGGGAGCGCGGCCGCCGGTGCTGAAGCCGCCGCCGTCGATGACTAGGGTTCGGGTGATTGACGATGGATGCTCGACTTGGGACATTTTTAGGGACTTGGCGCCGGAAGAAGAGAGGGAAATGGTGCGGATAAGGTTTGCTCCTTCTTCTTCTTCTTCTGATGAAGAAGAAGAAGAAGAACTAGAAGAAGAACTTGAAGAAGGAGAGGATGTTGTGGCGGATGAGATTAGAGAGACTGTGGAAAACTCCGGCGGGTGTTCGTTTACTACTTCCAATGACGACGACTCTTCCAGCACTACCACAGATCCTTCGCCGAATGGGAAGCCTAAGCTTGTTGTCACTAGTTGGGAGAAGGGTGATCTTCTCGGCAGCGGCTCTTTCGGGTCGGTGTATGAAGGAATTTCTGATGGTGGATGCTTCATCGCTGTTAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGAAGGCAAAGAGTTTCTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATCAATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAGACGTACCATCTTACAGATTCACATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCGAAGAGTGATTCACAGGGACATTAAATGTGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCAGACTTTGGATTGGCAAAGACTATCAAATTGAACGACATAAAATCTTGCCAAGGAACTGCATACTGGATGGCACCTGAGGTTGTTAATCGTAAAAGTCAAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACTCTAATCTCGAATGGATGCAGGCATTATGGAAAATTGGAAAGGGGGAGCCCCCTCTGGTTCCTGATTCTCTTTCAAAAGAGGCGCAAGATTTCATCCGTCTATGCTTACAAGTTAAGCCAGATAATCGTCCCACTGCTGCTCAGCTTTTAAAACATCCATTTGTAAATAAGCCCCTTCTTCCCACGTCTTCTGGATCAGTATCTCCTCACAATCACCACAGGCAGACTCTATTTGACCTAAAGAAGCAGGACGACAACTCATCCATGTTCTATAATGTTCTCCGGAATCTGGTGTTCAGCCATCAATCTTGGCTTACGCTGGTAGAGTGCATGCTTGTTATAACTTCAGTGATTTTCAAGGTCTACGCCAGATTTGGTAATGGGATATATGACTTTAGAAGAACAAGCATCTTTTTGTCACAGGTGCCTGCTCTGAACATCATCTTGCACGTAGAGCAATCCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

638

Amino Acids

70.97

Weight (kDa)

5.2

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 285 - 537 5.3e-69 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 287 - 533 1.2e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1787
AccB1I GGYRCC 3 cut(s) 604, 1342, 1870
AccBSI CCGCTC 1 cut(s) 459
AccI GTMKAC 3 cut(s) 425, 1158, 1809
AccII CGCG 4 cut(s) 96, 113, 130, 514
AccIII TCCGGA 1 cut(s) 1728
AclWI GGATC 6 cut(s) 12, 262, 803, 1068, 1264, 1648
AcoI YGGCCR 2 cut(s) 417, 515
AcsI RAATTY 1 cut(s) 903
AcuI CTGAAG 3 cut(s) 548, 1205, 1776
AcyI GRCGYC 2 cut(s) 72, 605
AfaI GTAC 4 cut(s) 311, 469, 1130, 1411
AfiI CCNNNNNNNGG 3 cut(s) 49, 162, 1735
AflIII ACRYGT 1 cut(s) 1147
AgsI TTSAA 7 cut(s) 701, 992, 1022, 1112, 1303, 1521, 1804
AhlI ACTAGT 1 cut(s) 845
AjiI CACGTC 1 cut(s) 1632
AjnI CCWGG 2 cut(s) 251, 963
AluBI AGCT 9 cut(s) 121, 159, 208, 835, 1072, 1117, 1267, 1388, 1591
AluI AGCT 9 cut(s) 121, 159, 208, 835, 1072, 1117, 1267, 1388, 1591
Alw26I GTCTC 3 cut(s) 322, 731, 1156
AlwI GGATC 6 cut(s) 12, 262, 803, 1068, 1264, 1648
Ama87I CYCGRG 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 1728
AoxI GGCC 7 cut(s) 15, 220, 249, 417, 452, 515, 1378
ApeKI GCWGC 4 cut(s) 115, 118, 873, 1583
ApoI RAATTY 1 cut(s) 903
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspLEI GCGC 7 cut(s) 42, 115, 132, 203, 514, 607, 1531
AspS9I GGNCC 7 cut(s) 16, 148, 153, 221, 273, 432, 452
AsuC2I CCSGG 3 cut(s) 19, 164, 262
AsuHPI GGTGA 4 cut(s) 208, 571, 872, 1652
AsuII TTCGAA 1 cut(s) 189
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 4 cut(s) 148, 153, 273, 432
AxyI CCTNAGG 1 cut(s) 1347
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 3 cut(s) 604, 1342, 1870
BanII GRGCYC 1 cut(s) 1497
BbsI GAAGAC 1 cut(s) 1626
BbvI GCAGC 4 cut(s) 127, 130, 885, 1570
BccI CCATC 7 cut(s) 310, 563, 905, 1140, 1333, 1429, 1755
BceAI ACGGC 3 cut(s) 196, 353, 523
BciT130I CCWGG 2 cut(s) 253, 965
BclI TGATCA 1 cut(s) 952
BcnI CCSGG 3 cut(s) 19, 164, 262
BcoDI GTCTC 3 cut(s) 322, 731, 1156
BcuI ACTAGT 1 cut(s) 845
BfaI CTAG 4 cut(s) 549, 686, 846, 1164
BfoI RGCGCY 2 cut(s) 43, 608
BglII AGATCT 1 cut(s) 1170
BlpI GCTNAGC 1 cut(s) 1587
Bme1390I CCNGG 5 cut(s) 19, 164, 253, 262, 965
Bme18I GGWCC 4 cut(s) 148, 153, 273, 432
BmeT110I CYCGRG 1 cut(s) 242
BmgBI CACGTC 1 cut(s) 1632
BmgT120I GGNCC 7 cut(s) 16, 148, 153, 221, 273, 432, 452
BmiI GGNNCC 8 cut(s) 6, 606, 1105, 1344, 1440, 1494, 1506, 1872
BmrFI CCNGG 5 cut(s) 19, 164, 253, 262, 965
BmrI ACTGGG 1 cut(s) 1440
BmsI GCATC 5 cut(s) 355, 563, 908, 1452, 1863
BmuI ACTGGG 1 cut(s) 1440
BpiI GAAGAC 1 cut(s) 1626
Bpu10I CCTNAGC 1 cut(s) 831
Bpu1102I GCTNAGC 1 cut(s) 1587
Bpu14I TTCGAA 1 cut(s) 189
BpuMI CCSGG 3 cut(s) 19, 164, 262
BsaAI YACGTR 2 cut(s) 313, 1897
BsaBI GATNNNNATC 1 cut(s) 1645
BsaHI GRCGYC 2 cut(s) 72, 605
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 5 cut(s) 243, 252, 369, 964, 1321
BsaWI WCCGGW 3 cut(s) 150, 270, 1728
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 162, 1735
Bse118I RCCGGY 2 cut(s) 218, 520
Bse1I ACTGG 2 cut(s) 1340, 1435
Bse21I CCTNAGG 1 cut(s) 1347
Bse3DI GCAATG 1 cut(s) 52
Bse8I GATNNNNATC 1 cut(s) 1645
BseAI TCCGGA 1 cut(s) 1728
BseBI CCWGG 2 cut(s) 253, 965
BseDI CCNNGG 5 cut(s) 243, 252, 369, 964, 1321
BseJI GATNNNNATC 1 cut(s) 1645
BseLI CCNNNNNNNGG 3 cut(s) 49, 162, 1735
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 3 cut(s) 1001, 1338, 1601
BseNI ACTGG 2 cut(s) 1340, 1435
BseRI GAGGAG 3 cut(s) 76, 79, 1641
BseX3I CGGCCG 1 cut(s) 515
BseXI GCAGC 4 cut(s) 127, 130, 885, 1570
BseYI CCCAGC 2 cut(s) 208, 449
Bsh1236I CGCG 4 cut(s) 96, 113, 130, 514
Bsh1285I CGRYCG 2 cut(s) 518, 1201
BshFI GGCC 7 cut(s) 17, 222, 251, 419, 454, 517, 1380
BshNI GGYRCC 3 cut(s) 604, 1342, 1870
BsiEI CGRYCG 2 cut(s) 518, 1201
BsiHKCI CYCGRG 1 cut(s) 242
BslFI GGGAC 5 cut(s) 244, 599, 611, 1232, 1560
BslI CCNNNNNNNGG 3 cut(s) 49, 162, 1735
BsmAI GTCTC 3 cut(s) 322, 731, 1156
BsmFI GGGAC 5 cut(s) 244, 599, 611, 1232, 1560
BsnI GGCC 7 cut(s) 17, 222, 251, 419, 454, 517, 1380
Bso31I GGTCTC 1 cut(s) 322
BsoBI CYCGRG 1 cut(s) 242
Bsp119I TTCGAA 1 cut(s) 189
Bsp1286I GDGCHC 1 cut(s) 1497
Bsp13I TCCGGA 1 cut(s) 1728
Bsp143I GATC 9 cut(s) 4, 267, 808, 862, 952, 1060, 1170, 1256, 1640
Bsp1720I GCTNAGC 1 cut(s) 1587
BspANI GGCC 7 cut(s) 17, 222, 251, 419, 454, 517, 1380
BspCNI CTCAG 3 cut(s) 1002, 1339, 1600
BspEI TCCGGA 1 cut(s) 1728
BspFNI CGCG 4 cut(s) 96, 113, 130, 514
BspLI GGNNCC 8 cut(s) 6, 606, 1105, 1344, 1440, 1494, 1506, 1872
BspPI GGATC 6 cut(s) 12, 262, 803, 1068, 1264, 1648
BspQI GCTCTTC 1 cut(s) 1011
BspT104I TTCGAA 1 cut(s) 189
BspT107I GGYRCC 3 cut(s) 604, 1342, 1870
BspTNI GGTCTC 1 cut(s) 322
BsrBI CCGCTC 1 cut(s) 459
BsrDI GCAATG 1 cut(s) 52
BsrFI RCCGGY 2 cut(s) 218, 520
BsrI ACTGG 2 cut(s) 1340, 1435
BssAI RCCGGY 2 cut(s) 218, 520
BssECI CCNNGG 5 cut(s) 243, 252, 369, 964, 1321
BssMI GATC 9 cut(s) 4, 267, 808, 862, 952, 1060, 1170, 1256, 1640
BssNAI GTATAC 1 cut(s) 1159
BssNI GRCGYC 2 cut(s) 72, 605
BssT1I CCWWGG 1 cut(s) 1321
Bst1107I GTATAC 1 cut(s) 1159
Bst2UI CCWGG 2 cut(s) 253, 965
Bst4CI ACNGT 5 cut(s) 309, 409, 415, 742, 1414
Bst6I CTCTTC 5 cut(s) 82, 609, 796, 1011, 1198
BstACI GRCGYC 2 cut(s) 72, 605
BstBAI YACGTR 2 cut(s) 313, 1897
BstBI TTCGAA 1 cut(s) 189
BstC8I GCNNGC 6 cut(s) 134, 1248, 1269, 1467, 1778, 1875
BstDEI CTNAG 4 cut(s) 831, 1010, 1347, 1587
BstDSI CCRYGG 1 cut(s) 369
BstFNI CGCG 4 cut(s) 96, 113, 130, 514
BstH2I RGCGCY 2 cut(s) 43, 608
BstHHI GCGC 7 cut(s) 42, 115, 132, 203, 514, 607, 1531
BstKTI GATC 9 cut(s) 7, 270, 811, 865, 955, 1063, 1173, 1259, 1643
BstMAI GTCTC 3 cut(s) 322, 731, 1156
BstMBI GATC 9 cut(s) 4, 267, 808, 862, 952, 1060, 1170, 1256, 1640
BstMCI CGRYCG 2 cut(s) 518, 1201
BstMWI GCNNNNNNNGC 5 cut(s) 127, 219, 523, 532, 1900
BstNI CCWGG 2 cut(s) 253, 965
BstNSI RCATGY 3 cut(s) 1151, 1250, 1780
BstSCI CCNGG 5 cut(s) 17, 162, 251, 260, 963
BstUI CGCG 4 cut(s) 96, 113, 130, 514
BstV1I GCAGC 4 cut(s) 127, 130, 885, 1570
BstV2I GAAGAC 1 cut(s) 1626
BstX2I RGATCY 5 cut(s) 4, 267, 808, 1170, 1256
BstYI RGATCY 5 cut(s) 4, 267, 808, 1170, 1256
BstZ17I GTATAC 1 cut(s) 1159
BstZI CGGCCG 1 cut(s) 515
Bsu36I CCTNAGG 1 cut(s) 1347
BsuRI GGCC 7 cut(s) 17, 222, 251, 419, 454, 517, 1380
BtgI CCRYGG 1 cut(s) 369
BtgZI GCGATG 1 cut(s) 910
BtrI CACGTC 1 cut(s) 1632
BtsI GCAGTG 1 cut(s) 1578
BtsIMutI CAGTG 2 cut(s) 1578, 1800
Cac8I GCNNGC 6 cut(s) 134, 1248, 1269, 1467, 1778, 1875
CciNI GCGGCCGC 1 cut(s) 515
CfoI GCGC 7 cut(s) 42, 115, 132, 203, 514, 607, 1531
Cfr10I RCCGGY 2 cut(s) 218, 520
Cfr13I GGNCC 7 cut(s) 16, 148, 153, 221, 273, 432, 452
CpoI CGGWCCG 2 cut(s) 148, 432
CseI GACGC 1 cut(s) 80
Csp6I GTAC 4 cut(s) 310, 468, 1129, 1410
CspI CGGWCCG 2 cut(s) 148, 432
CviAII CATG 6 cut(s) 356, 1025, 1148, 1247, 1711, 1777
CviQI GTAC 4 cut(s) 310, 468, 1129, 1410
DdeI CTNAG 4 cut(s) 831, 1010, 1347, 1587
DinI GGCGCC 1 cut(s) 606
DpnI GATC 9 cut(s) 6, 269, 810, 864, 954, 1062, 1172, 1258, 1642
DpnII GATC 9 cut(s) 4, 267, 808, 862, 952, 1060, 1170, 1256, 1640
DraI TTTAAA 1 cut(s) 1596
EaeI YGGCCR 2 cut(s) 417, 515
EagI CGGCCG 1 cut(s) 515
Eam1104I CTCTTC 5 cut(s) 82, 609, 796, 1011, 1198
EarI CTCTTC 5 cut(s) 82, 609, 796, 1011, 1198
EciI GGCGGA 2 cut(s) 389, 737
EclXI CGGCCG 1 cut(s) 515
Eco130I CCWWGG 1 cut(s) 1321
Eco24I GRGCYC 1 cut(s) 1497
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 4 cut(s) 148, 153, 273, 432
Eco52I CGGCCG 1 cut(s) 515
Eco57I CTGAAG 3 cut(s) 548, 1205, 1776
Eco81I CCTNAGG 1 cut(s) 1347
Eco88I CYCGRG 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 251, 963
EcoT14I CCWWGG 1 cut(s) 1321
EcoT38I GRGCYC 1 cut(s) 1497
EgeI GGCGCC 1 cut(s) 606
EheI GGCGCC 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 1321
FaeI CATG 6 cut(s) 359, 1028, 1151, 1250, 1714, 1780
FalI AAGNNNNNCTT 4 cut(s) 681, 713, 1092, 1124
FaqI GGGAC 5 cut(s) 244, 599, 611, 1232, 1560
FatI CATG 6 cut(s) 355, 1024, 1147, 1246, 1710, 1776
FauI CCCGC 3 cut(s) 101, 452, 749
FbaI TGATCA 1 cut(s) 952
FblI GTMKAC 3 cut(s) 425, 1158, 1809
FriOI GRGCYC 1 cut(s) 1497
FspBI CTAG 4 cut(s) 549, 686, 846, 1164
GlaI GCGC 7 cut(s) 41, 114, 131, 202, 513, 606, 1530
GsaI CCCAGC 2 cut(s) 212, 453
HaeII RGCGCY 2 cut(s) 43, 608
HaeIII GGCC 7 cut(s) 17, 222, 251, 419, 454, 517, 1380
HgaI GACGC 1 cut(s) 80
HhaI GCGC 7 cut(s) 42, 115, 132, 203, 514, 607, 1531
Hin1I GRCGYC 2 cut(s) 72, 605
Hin1II CATG 6 cut(s) 359, 1028, 1151, 1250, 1714, 1780
Hin6I GCGC 7 cut(s) 40, 113, 130, 201, 512, 605, 1529
HinP1I GCGC 7 cut(s) 40, 113, 130, 201, 512, 605, 1529
HincII GTYRAC 2 cut(s) 426, 1428
HindII GTYRAC 2 cut(s) 426, 1428
HindIII AAGCTT 2 cut(s) 833, 1265
HpaI GTTAAC 1 cut(s) 1428
HphI GGTGA 4 cut(s) 208, 571, 872, 1652
Hpy166II GTNNAC 6 cut(s) 276, 426, 768, 1159, 1428, 1810
Hpy188III TCNNGA 6 cut(s) 1085, 1196, 1454, 1508, 1638, 1729
Hpy8I GTNNAC 6 cut(s) 276, 426, 768, 1159, 1428, 1810
Hpy99I CGWCG 3 cut(s) 74, 544, 788
HpyCH4III ACNGT 5 cut(s) 309, 409, 415, 742, 1414
HpyCH4IV ACGT 4 cut(s) 312, 1127, 1631, 1896
HpyCH4V TGCA 8 cut(s) 1177, 1232, 1246, 1271, 1331, 1465, 1776, 1894
HpyF10VI GCNNNNNNNGC 5 cut(s) 127, 219, 523, 532, 1900
HpyF3I CTNAG 4 cut(s) 831, 1010, 1347, 1587
HpySE526I ACGT 4 cut(s) 312, 1127, 1631, 1896
Hsp92I GRCGYC 2 cut(s) 72, 605
Hsp92II CATG 6 cut(s) 359, 1028, 1151, 1250, 1714, 1780
HspAI GCGC 7 cut(s) 40, 113, 130, 201, 512, 605, 1529
KasI GGCGCC 1 cut(s) 604
Kpn2I TCCGGA 1 cut(s) 1728
Ksp22I TGATCA 1 cut(s) 952
KspAI GTTAAC 1 cut(s) 1428
Kzo9I GATC 9 cut(s) 4, 267, 808, 862, 952, 1060, 1170, 1256, 1640
LguI GCTCTTC 1 cut(s) 1011
LmnI GCTCC 7 cut(s) 37, 126, 352, 509, 649, 1109, 1492
Lsp1109I GCAGC 4 cut(s) 127, 130, 885, 1570
LweI GCATC 5 cut(s) 355, 563, 908, 1452, 1863
MaeI CTAG 4 cut(s) 549, 686, 846, 1164
MaeII ACGT 4 cut(s) 312, 1127, 1631, 1896
MaeIII GTNAC 5 cut(s) 403, 409, 841, 1093, 1863
MalI GATC 9 cut(s) 6, 269, 810, 864, 954, 1062, 1172, 1258, 1642
MbiI CCGCTC 1 cut(s) 459
MboI GATC 9 cut(s) 4, 267, 808, 862, 952, 1060, 1170, 1256, 1640
MflI RGATCY 5 cut(s) 4, 267, 808, 1170, 1256
MhlI GDGCHC 1 cut(s) 1497
MluCI AATT 5 cut(s) 377, 501, 903, 1298, 1479
Mly113I GGCGCC 1 cut(s) 605
MlyI GAGTC 9 cut(s) 266, 421, 431, 446, 452, 782, 1021, 1408, 1666
MmeI TCCRAC 4 cut(s) 15, 126, 478, 1398
MroI TCCGGA 1 cut(s) 1728
MseI TTAA 7 cut(s) 504, 933, 1224, 1356, 1427, 1560, 1595
MslI CAYNNNNRTG 2 cut(s) 360, 1251
MspA1I CMGCKG 2 cut(s) 876, 1388
MspR9I CCNGG 5 cut(s) 19, 164, 253, 262, 965
MvaI CCWGG 2 cut(s) 253, 965
MvnI CGCG 4 cut(s) 96, 113, 130, 514
MwoI GCNNNNNNNGC 5 cut(s) 127, 219, 523, 532, 1900
NarI GGCGCC 1 cut(s) 605
NciI CCSGG 3 cut(s) 19, 164, 262
NdeII GATC 9 cut(s) 4, 267, 808, 862, 952, 1060, 1170, 1256, 1640
NlaIII CATG 6 cut(s) 359, 1028, 1151, 1250, 1714, 1780
NlaIV GGNNCC 8 cut(s) 6, 606, 1105, 1344, 1440, 1494, 1506, 1872
NmeAIII GCCGAG 4 cut(s) 229, 445, 480, 849
NmuCI GTSAC 2 cut(s) 841, 1863
NotI GCGGCCGC 1 cut(s) 515
NspI RCATGY 3 cut(s) 1151, 1250, 1780
NspV TTCGAA 1 cut(s) 189
PaeI GCATGC 2 cut(s) 1250, 1780
PciI ACATGT 1 cut(s) 1147
PciSI GCTCTTC 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 3 cut(s) 186, 240, 452
PfeI GAWTC 5 cut(s) 347, 1142, 1210, 1511, 1732
PflFI GACNNNGTC 1 cut(s) 430
PfoI TCCNGGA 1 cut(s) 260
PleI GAGTC 9 cut(s) 265, 421, 430, 445, 451, 782, 1020, 1407, 1666
PluTI GGCGCC 1 cut(s) 608
PpsI GAGTC 9 cut(s) 265, 421, 430, 445, 451, 782, 1020, 1407, 1666
Ppu21I YACGTR 2 cut(s) 313, 1897
PscI ACATGT 1 cut(s) 1147
PsiI TTATAA 1 cut(s) 1787
Psp6I CCWGG 2 cut(s) 251, 963
PspFI CCCAGC 2 cut(s) 208, 449
PspGI CCWGG 2 cut(s) 251, 963
PspN4I GGNNCC 8 cut(s) 6, 606, 1105, 1344, 1440, 1494, 1506, 1872
PspPI GGNCC 7 cut(s) 16, 148, 153, 221, 273, 432, 452
PsuI RGATCY 5 cut(s) 4, 267, 808, 1170, 1256
PsyI GACNNNGTC 1 cut(s) 430
PvuII CAGCTG 1 cut(s) 1388
RsaI GTAC 4 cut(s) 311, 469, 1130, 1411
RsaNI GTAC 4 cut(s) 310, 468, 1129, 1410
RseI CAYNNNNRTG 2 cut(s) 360, 1251
Rsr2I CGGWCCG 2 cut(s) 148, 432
RsrII CGGWCCG 2 cut(s) 148, 432
SalI GTCGAC 1 cut(s) 424
SapI GCTCTTC 1 cut(s) 1011
SaqAI TTAA 7 cut(s) 504, 933, 1224, 1356, 1427, 1560, 1595
Sau3AI GATC 9 cut(s) 4, 267, 808, 862, 952, 1060, 1170, 1256, 1640
Sau96I GGNCC 7 cut(s) 16, 148, 153, 221, 273, 432, 452
SchI GAGTC 9 cut(s) 266, 421, 431, 446, 452, 782, 1021, 1408, 1666
ScrFI CCNGG 5 cut(s) 19, 164, 253, 262, 965
SduI GDGCHC 1 cut(s) 1497
SfaNI GCATC 5 cut(s) 355, 563, 908, 1452, 1863
SfoI GGCGCC 1 cut(s) 606
SfuI TTCGAA 1 cut(s) 189
SgrAI CRCCGGYG 1 cut(s) 520
SinI GGWCC 4 cut(s) 148, 153, 273, 432
SmiMI CAYNNNNRTG 2 cut(s) 360, 1251
SpeI ACTAGT 1 cut(s) 845
SphI GCATGC 2 cut(s) 1250, 1780
Sse9I AATT 5 cut(s) 377, 501, 903, 1298, 1479
SspDI GGCGCC 1 cut(s) 604
SspMI CTAG 4 cut(s) 549, 686, 846, 1164
StyD4I CCNGG 5 cut(s) 17, 162, 251, 260, 963
StyI CCWWGG 1 cut(s) 1321
TaaI ACNGT 5 cut(s) 309, 409, 415, 742, 1414
TaiI ACGT 4 cut(s) 315, 1130, 1634, 1899
TaqI TCGA 6 cut(s) 138, 189, 425, 542, 578, 1455
TaqII GACCGA 2 cut(s) 420, 1215
TasI AATT 5 cut(s) 377, 501, 903, 1298, 1479
TatI WGTACW 2 cut(s) 467, 1409
TauI GCSGC 7 cut(s) 45, 251, 517, 520, 535, 538, 879
TfiI GAWTC 5 cut(s) 347, 1142, 1210, 1511, 1732
Tru1I TTAA 7 cut(s) 504, 933, 1224, 1356, 1427, 1560, 1595
Tru9I TTAA 7 cut(s) 504, 933, 1224, 1356, 1427, 1560, 1595
TscAI CASTG 2 cut(s) 1585, 1800
TseFI GTSAC 2 cut(s) 841, 1863
TseI GCWGC 4 cut(s) 115, 118, 873, 1583
Tsp45I GTSAC 2 cut(s) 841, 1863
TspDTI ATGAA 4 cut(s) 681, 912, 913, 1528
TspGWI ACGGA 2 cut(s) 208, 1532
TspRI CASTG 2 cut(s) 1585, 1800
Tth111I GACNNNGTC 1 cut(s) 430
VpaK11BI GGWCC 4 cut(s) 148, 153, 273, 432
XapI RAATTY 1 cut(s) 903
XceI RCATGY 3 cut(s) 1151, 1250, 1780
XcmI CCANNNNNNNNNTGG 1 cut(s) 1392
XmiI GTMKAC 3 cut(s) 425, 1158, 1809
XspI CTAG 4 cut(s) 549, 686, 846, 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.