Rroxscaffold_3G00219400

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000243
Physical Location & Seq
Reverse (-)
1516869 .. 1522752
5884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219400.1

Sequence Viewer

Length: 1659 bp
ATGCATCACTTACCGCGTCTTTTCTCTAGCAGCAAGGATCGAACTAAATCCATGGATCCGAAGAAGGCCCGGCGGTTGCAGCGCCGCAATGCGGTCAAGCACATTGACTACGACGCCTCTTCGTCCTCCTCCTCCCGCGACCACTCCACCCACGCGCAGCAGCTCCACACGCGCTCGCTCGACCTGTCGGACCGGACCAGCTTCCGGGTCGAAGGAAATGACGGCGAGTTCGAACGGATTTGCGCCGAGCTGGGCTTCTCCGGCCCAGACGACTTCGCGATACCCGAGGCGGCCTGGGAGTCCCGGAGGATCCGCTCGACCAACTCGGATGTTCTCCCCCTGTCAAAACTGTACCCGATGGATAGTCCGAGGCCCGACCCGAAGGACGAATCGGAGCATGAGGATGATGCCGTGGCGGAATTGCGCAATAGAGTTAGGGATAGCGTAACTGTTACGGTGGCCGAGTCGACTCGGTCCGAGTCGACGGGGCCGAGCGGGTGCTGTACTGCGAGTAGTAGTTGTAGTGGTAGTGTCGGAATTAAGGGAGCGCGGCCGCCGGAGCTGAAGCCGCCGCCGTCGATGACTAGGGTTCCGGTGATTGACAATGGATGCTCGACTTGGGACATTTTGAGGGACTTCGCTCCAGAAGAAGAGAGGGATATGGTGCGGAGCAGGTTTGTTCCCTCTTCTTCTTCTGATGAAGAAGATGAAGAACAAGAACTAGAAGAAGAACTTGAAGAAGGAGAGGATGTTGTGGCGGATGAGATTAGAGAAACCGTGGCAAACTCCGGCGGGTGTTCGTTTACTACTTCCAATGACGACGACTCTTCCAGCACTACCACAGATCCTTCCAATATTTCTCCAAATGGAAGGTTTTCGCCGAATGGGAAGCCTAAGCTTATTGTCACTAGTTGGGAGAAGGGCGATCTTCTCGGCAGCGGCTCTTTCGGGTCTGTGTATGAAGGAATTTCTGATGGCGGATGCTTCATTGCTGTTAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGAAGGCAAAGAGTTTCTCAACTTGAACAGGAGATTGCTCTTCTGAGTCAGTTTGAACATGAGAACATAGTTCATTATTATGGCACACAAAAGGATGAATCAAAGCTCTATATCTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAGACGTACCATCTTACAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCATGACCGAAGAGTGATTCACAGGGACGTTAAATGTGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCAGACTTTGGATTGGCAAAGACTATCAAAATGAATGACATAAAATCTTGCCAAGGAACTGCATTCTGGATGGCACCTGAGGTTGTTAATCGTAAGAGTCAAGGTTATGGCCTTCCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACTCTAATCTTGAATGGATGCAGGCATTATGGAAAATTGGAAAGGGGAGCCCCCTCTGGTTCCTGATTCTCTTTCAAAAGAGGCACAAGATTTCATCCGTCTATGCTTACAAGTTAAGCCAGATAACCGTCCCACTGCTGCTCAGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

552

Amino Acids

61.53

Weight (kDa)

5.5

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 306 - 516 2.1e-58 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 308 - 519 1.9e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08470 AT4G08470 AT4G08470 AT4G08480 AT4G08500
fragaria_vesca FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_2g26162 FvH4_3g43920 FvH4_3g44020 FvH4_5g29850 FvH4_5g38970
malus_domestica MD02G1097900.v1.1 MD08G1173000.v1.1 MD08G1227000.v1.1 MD15G1358300.v1.1 MD15G1419300.v1.1
prunus_persica Prupe.1G505700_v2.0.a1 Prupe.1G505700_v2.0.a1 Prupe.1G505800_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.1G564100_v2.0.a1 Prupe.7G007900_v2.0.a1
pyrus_communis pycom08g14750 pycom08g19800 pycom15g32060 pycom15g37220
rosa_chinensis RchiOBHm_Chr1g0352161 RchiOBHm_Chr5g0079491 RchiOBHm_Chr5g0080131 RchiOBHm_Chr5g0080301 RchiOBHm_Chr6g0294571 RchiOBHm_Chr6g0294581 RchiOBHm_Chr7g0223451 RchiOBHm_Chr7g0240941
rosa_laevigata RLG00000000612 RLG00000001964 RLG00000011858 RLG00000011859 RLG00000013468 RLG00000028354 RLG00000035064 RLG00000035074 RLG00000036819 RLG00000036822 RLG00000036849
rosa_multiflora Rmu_co7998012.1_g000001 Rmu_co8188232.1_g000001 Rmu_sc0001324.1_g000001 Rmu_sc0001599.1_g000021 Rmu_sc0006084.1_g000016 Rmu_sc0006264.1_g000020 Rmu_sc0007408.1_g000005 Rmu_sc0007806.1_g000004 Rmu_sc0008749.1_g000001 Rmu_sc0008957.1_g000002 Rmu_sc0011551.1_g000001
rosa_roxburghii Rroxscaffold_1G00003460 Rroxscaffold_3G00219400 Rroxscaffold_3G00235690 Rroxscaffold_4G00303130 Rroxscaffold_5G00380490 Rroxscaffold_7G00173210 Rroxscaffold_7G00173220
rosa_rugosa Rorug01G0225400 Rorug01G0225500 Rorug01G0225600 Rorug05G0464600 Rorug05G0464700 Rorug05G0465900 Rorug05G0467900 Rorug06G0246100 Rorug06G0246100 Rorug07G0217300 Rorug07G0217300 Rorug07G0340500
rosa_samantha Rh1AG239500 Rh1CG223900 Rh2CG299500 Rh2DG336700 Rh4BG236600 Rh5AG516900 Rh5AG517500 Rh5AG517700 Rh5AG518100 Rh5AG518500 Rh5AG519700 Rh5BG540800 Rh5CG563500 Rh5CG563700 Rh5DG038200 Rh6AG358400 Rh6BG365200 Rh6BG365300 Rh6BG365500 Rh7AG360500 Rh7AG495500 Rh7BG351100 Rh7BG466800 Rh7CG379000 Rh7CG379100 Rh7CG513000 Rh7DG356000
rosa_wichuraiana Rw0G023410 Rw1G020800 Rw5G048120 Rw5G048230 Rw5G048280 Rw5G048440 Rw6G031270 Rw7G029910 Rw7G030670 Rw7G041870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 425
Acc36I ACCTGC 1 cut(s) 663
AccB1I GGYRCC 1 cut(s) 1405
AccBSI CCGCTC 2 cut(s) 315, 495
AccI GTMKAC 3 cut(s) 467, 482, 1221
AccII CGCG 6 cut(s) 16, 138, 155, 172, 278, 550
AclWI GGATC 7 cut(s) 45, 50, 63, 304, 317, 839, 1327
AcoI YGGCCR 2 cut(s) 459, 551
AcsI RAATTY 1 cut(s) 966
AcuI CTGAAG 2 cut(s) 584, 1268
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 4 cut(s) 353, 505, 1193, 1474
AfiI CCNNNNNNNGG 2 cut(s) 91, 204
AgsI TTSAA 6 cut(s) 737, 1055, 1085, 1175, 1520, 1583
AhlI ACTAGT 1 cut(s) 908
AjnI CCWGG 2 cut(s) 293, 1026
Alw26I GTCTC 1 cut(s) 1219
AlwI GGATC 7 cut(s) 45, 50, 63, 304, 317, 839, 1327
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 8 cut(s) 66, 262, 291, 371, 459, 488, 551, 1441
ApeKI GCWGC 6 cut(s) 30, 79, 157, 160, 936, 1645
ApoI RAATTY 1 cut(s) 966
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
Asp700I GAANNNNTTC 1 cut(s) 874
AspLEI GCGC 6 cut(s) 84, 157, 174, 245, 426, 550
AspS9I GGNCC 7 cut(s) 67, 190, 195, 263, 372, 474, 488
AsuC2I CCSGG 3 cut(s) 70, 206, 304
AsuHPI GGTGA 1 cut(s) 607
AsuII TTCGAA 1 cut(s) 231
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 3 cut(s) 190, 195, 474
AxyI CCTNAGG 1 cut(s) 1410
BamHI GGATCC 2 cut(s) 55, 309
BanI GGYRCC 1 cut(s) 1405
BanII GRGCYC 1 cut(s) 1559
BbvI GCAGC 6 cut(s) 42, 91, 169, 172, 948, 1632
BccI CCATC 5 cut(s) 352, 968, 1203, 1396, 1492
BceAI ACGGC 3 cut(s) 238, 395, 559
BciT130I CCWGG 2 cut(s) 295, 1028
BclI TGATCA 1 cut(s) 1015
BcnI CCSGG 3 cut(s) 70, 206, 304
BcoDI GTCTC 1 cut(s) 1219
BcuI ACTAGT 1 cut(s) 908
BfaI CTAG 5 cut(s) 27, 585, 722, 909, 1227
BfoI RGCGCY 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 663
BglII AGATCT 1 cut(s) 1233
BlpI GCTNAGC 1 cut(s) 1649
Bme1390I CCNGG 5 cut(s) 70, 206, 295, 304, 1028
Bme18I GGWCC 3 cut(s) 190, 195, 474
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 7 cut(s) 67, 190, 195, 263, 372, 474, 488
BmiI GGNNCC 9 cut(s) 57, 311, 489, 591, 1168, 1407, 1503, 1556, 1568
BmrFI CCNGG 5 cut(s) 70, 206, 295, 304, 1028
BmrI ACTGGG 1 cut(s) 1503
BmsI GCATC 5 cut(s) 13, 397, 599, 971, 1515
BmuI ACTGGG 1 cut(s) 1503
BpmI CTGGAG 1 cut(s) 627
Bpu10I CCTNAGC 1 cut(s) 894
Bpu1102I GCTNAGC 1 cut(s) 1649
Bpu14I TTCGAA 1 cut(s) 231
BpuMI CCSGG 3 cut(s) 70, 206, 304
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 8 cut(s) 51, 285, 294, 368, 411, 777, 1027, 1384
BsaWI WCCGGW 2 cut(s) 192, 592
BsaXI ACNNNNNCTCC 2 cut(s) 735, 765
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 204
Bse1I ACTGG 1 cut(s) 1498
Bse21I CCTNAGG 1 cut(s) 1410
Bse3DI GCAATG 2 cut(s) 94, 987
BseBI CCWGG 2 cut(s) 295, 1028
BseDI CCNNGG 8 cut(s) 51, 285, 294, 368, 411, 777, 1027, 1384
BseLI CCNNNNNNNGG 2 cut(s) 91, 204
BseMI GCAATG 2 cut(s) 94, 987
BseMII CTCAG 2 cut(s) 1064, 1401
BseNI ACTGG 1 cut(s) 1498
BseRI GAGGAG 2 cut(s) 118, 121
BseX3I CGGCCG 1 cut(s) 551
BseXI GCAGC 6 cut(s) 42, 91, 169, 172, 948, 1632
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 6 cut(s) 16, 138, 155, 172, 278, 550
Bsh1285I CGRYCG 1 cut(s) 554
BshFI GGCC 8 cut(s) 68, 264, 293, 373, 461, 490, 553, 1443
BshNI GGYRCC 1 cut(s) 1405
BsiEI CGRYCG 1 cut(s) 554
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 8 cut(s) 70, 193, 205, 261, 304, 557, 593, 789
BslFI GGGAC 5 cut(s) 286, 635, 647, 1295, 1622
BslI CCNNNNNNNGG 2 cut(s) 91, 204
BsmAI GTCTC 1 cut(s) 1219
BsmFI GGGAC 5 cut(s) 286, 635, 647, 1295, 1622
BsmI GAATGC 1 cut(s) 1394
BsnI GGCC 8 cut(s) 68, 264, 293, 373, 461, 490, 553, 1443
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 1559
Bsp143I GATC 8 cut(s) 37, 55, 309, 844, 925, 1015, 1233, 1319
Bsp1720I GCTNAGC 1 cut(s) 1649
Bsp19I CCATGG 1 cut(s) 51
Bsp68I TCGCGA 1 cut(s) 278
BspANI GGCC 8 cut(s) 68, 264, 293, 373, 461, 490, 553, 1443
BspCNI CTCAG 2 cut(s) 1065, 1402
BspFNI CGCG 6 cut(s) 16, 138, 155, 172, 278, 550
BspHI TCATGA 1 cut(s) 1258
BspLI GGNNCC 9 cut(s) 57, 311, 489, 591, 1168, 1407, 1503, 1556, 1568
BspMI ACCTGC 1 cut(s) 663
BspPI GGATC 7 cut(s) 45, 50, 63, 304, 317, 839, 1327
BspQI GCTCTTC 1 cut(s) 1074
BspT104I TTCGAA 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 1405
BsrBI CCGCTC 2 cut(s) 315, 495
BsrDI GCAATG 2 cut(s) 94, 987
BsrI ACTGG 1 cut(s) 1498
BssECI CCNNGG 8 cut(s) 51, 285, 294, 368, 411, 777, 1027, 1384
BssMI GATC 8 cut(s) 37, 55, 309, 844, 925, 1015, 1233, 1319
BssNAI GTATAC 1 cut(s) 1222
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 51, 1384
Bst1107I GTATAC 1 cut(s) 1222
Bst2UI CCWGG 2 cut(s) 295, 1028
Bst4CI ACNGT 6 cut(s) 351, 451, 457, 778, 1477, 1636
Bst6I CTCTTC 6 cut(s) 124, 645, 691, 832, 1074, 1261
BstACI GRCGYC 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 4 cut(s) 176, 1311, 1332, 1530
BstDEI CTNAG 4 cut(s) 894, 1073, 1410, 1649
BstDSI CCRYGG 3 cut(s) 51, 411, 777
BstFNI CGCG 6 cut(s) 16, 138, 155, 172, 278, 550
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 6 cut(s) 84, 157, 174, 245, 426, 550
BstKTI GATC 8 cut(s) 40, 58, 312, 847, 928, 1018, 1236, 1322
BstMAI GTCTC 1 cut(s) 1219
BstMBI GATC 8 cut(s) 37, 55, 309, 844, 925, 1015, 1233, 1319
BstMCI CGRYCG 1 cut(s) 554
BstMWI GCNNNNNNNGC 5 cut(s) 79, 169, 261, 559, 568
BstNI CCWGG 2 cut(s) 295, 1028
BstNSI RCATGY 2 cut(s) 1214, 1313
BstSCI CCNGG 5 cut(s) 68, 204, 293, 302, 1026
BstUI CGCG 6 cut(s) 16, 138, 155, 172, 278, 550
BstV1I GCAGC 6 cut(s) 42, 91, 169, 172, 948, 1632
BstX2I RGATCY 5 cut(s) 55, 309, 844, 1233, 1319
BstYI RGATCY 5 cut(s) 55, 309, 844, 1233, 1319
BstZ17I GTATAC 1 cut(s) 1222
BstZI CGGCCG 1 cut(s) 551
Bsu36I CCTNAGG 1 cut(s) 1410
BsuRI GGCC 8 cut(s) 68, 264, 293, 373, 461, 490, 553, 1443
BtgI CCRYGG 3 cut(s) 51, 411, 777
BtsI GCAGTG 1 cut(s) 1640
BtsIMutI CAGTG 1 cut(s) 1640
BtuMI TCGCGA 1 cut(s) 278
BveI ACCTGC 1 cut(s) 663
Cac8I GCNNGC 4 cut(s) 176, 1311, 1332, 1530
CciI TCATGA 1 cut(s) 1258
CciNI GCGGCCGC 1 cut(s) 551
CfoI GCGC 6 cut(s) 84, 157, 174, 245, 426, 550
Cfr13I GGNCC 7 cut(s) 67, 190, 195, 263, 372, 474, 488
CpoI CGGWCCG 2 cut(s) 190, 474
CseI GACGC 2 cut(s) 5, 122
Csp6I GTAC 4 cut(s) 352, 504, 1192, 1473
CspI CGGWCCG 2 cut(s) 190, 474
CviAII CATG 6 cut(s) 52, 398, 1088, 1211, 1259, 1310
CviQI GTAC 4 cut(s) 352, 504, 1192, 1473
DdeI CTNAG 4 cut(s) 894, 1073, 1410, 1649
DpnI GATC 8 cut(s) 39, 57, 311, 846, 927, 1017, 1235, 1321
DpnII GATC 8 cut(s) 37, 55, 309, 844, 925, 1015, 1233, 1319
EaeI YGGCCR 2 cut(s) 459, 551
EagI CGGCCG 1 cut(s) 551
Eam1104I CTCTTC 6 cut(s) 124, 645, 691, 832, 1074, 1261
EarI CTCTTC 6 cut(s) 124, 645, 691, 832, 1074, 1261
EciI GGCGGA 3 cut(s) 431, 773, 993
EclXI CGGCCG 1 cut(s) 551
Eco130I CCWWGG 2 cut(s) 51, 1384
Eco24I GRGCYC 1 cut(s) 1559
Eco47I GGWCC 3 cut(s) 190, 195, 474
Eco52I CGGCCG 1 cut(s) 551
Eco57I CTGAAG 2 cut(s) 584, 1268
Eco81I CCTNAGG 1 cut(s) 1410
Eco88I CYCGRG 1 cut(s) 284
EcoRII CCWGG 2 cut(s) 293, 1026
EcoT14I CCWWGG 2 cut(s) 51, 1384
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1559
ErhI CCWWGG 2 cut(s) 51, 1384
FaeI CATG 6 cut(s) 55, 401, 1091, 1214, 1262, 1313
FalI AAGNNNNNCTT 4 cut(s) 717, 749, 1155, 1187
FaqI GGGAC 5 cut(s) 286, 635, 647, 1295, 1622
FatI CATG 6 cut(s) 51, 397, 1087, 1210, 1258, 1309
FauI CCCGC 3 cut(s) 143, 488, 785
FbaI TGATCA 1 cut(s) 1015
FblI GTMKAC 3 cut(s) 467, 482, 1221
FriOI GRGCYC 1 cut(s) 1559
FspBI CTAG 5 cut(s) 27, 585, 722, 909, 1227
FspI TGCGCA 1 cut(s) 425
GlaI GCGC 6 cut(s) 83, 156, 173, 244, 425, 549
GsaI CCCAGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 627
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 8 cut(s) 68, 264, 293, 373, 461, 490, 553, 1443
HapII CCGG 8 cut(s) 70, 193, 205, 261, 304, 557, 593, 789
HgaI GACGC 2 cut(s) 5, 122
HhaI GCGC 6 cut(s) 84, 157, 174, 245, 426, 550
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 6 cut(s) 55, 401, 1091, 1214, 1262, 1313
Hin6I GCGC 6 cut(s) 82, 155, 172, 243, 424, 548
HinP1I GCGC 6 cut(s) 82, 155, 172, 243, 424, 548
HincII GTYRAC 3 cut(s) 468, 483, 1491
HindII GTYRAC 3 cut(s) 468, 483, 1491
HindIII AAGCTT 2 cut(s) 896, 1328
HpaI GTTAAC 1 cut(s) 1491
HpaII CCGG 8 cut(s) 70, 193, 205, 261, 304, 557, 593, 789
HphI GGTGA 1 cut(s) 607
Hpy166II GTNNAC 5 cut(s) 468, 483, 804, 1222, 1491
Hpy188III TCNNGA 7 cut(s) 277, 644, 1148, 1259, 1399, 1517, 1570
Hpy8I GTNNAC 5 cut(s) 468, 483, 804, 1222, 1491
Hpy99I CGWCG 4 cut(s) 116, 487, 580, 824
HpyCH4III ACNGT 6 cut(s) 351, 451, 457, 778, 1477, 1636
HpyCH4IV ACGT 2 cut(s) 1190, 1284
HpyCH4V TGCA 8 cut(s) 4, 79, 1240, 1295, 1309, 1334, 1394, 1528
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 169, 261, 559, 568
HpyF3I CTNAG 4 cut(s) 894, 1073, 1410, 1649
HpySE526I ACGT 2 cut(s) 1190, 1284
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 6 cut(s) 55, 401, 1091, 1214, 1262, 1313
HspAI GCGC 6 cut(s) 82, 155, 172, 243, 424, 548
Ksp22I TGATCA 1 cut(s) 1015
KspAI GTTAAC 1 cut(s) 1491
Kzo9I GATC 8 cut(s) 37, 55, 309, 844, 925, 1015, 1233, 1319
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 8 cut(s) 168, 394, 545, 559, 646, 669, 1172, 1554
Lsp1109I GCAGC 6 cut(s) 42, 91, 169, 172, 948, 1632
LweI GCATC 5 cut(s) 13, 397, 599, 971, 1515
MaeI CTAG 5 cut(s) 27, 585, 722, 909, 1227
MaeII ACGT 2 cut(s) 1190, 1284
MaeIII GTNAC 4 cut(s) 445, 451, 904, 1156
MalI GATC 8 cut(s) 39, 57, 311, 846, 927, 1017, 1235, 1321
MbiI CCGCTC 2 cut(s) 315, 495
MboI GATC 8 cut(s) 37, 55, 309, 844, 925, 1015, 1233, 1319
MflI RGATCY 5 cut(s) 55, 309, 844, 1233, 1319
MhlI GDGCHC 1 cut(s) 1559
MluCI AATT 4 cut(s) 419, 537, 966, 1542
MlyI GAGTC 8 cut(s) 308, 463, 473, 488, 818, 1084, 1438, 1471
MmeI TCCRAC 3 cut(s) 168, 514, 1461
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 874
MseI TTAA 7 cut(s) 540, 996, 1287, 1419, 1490, 1622, 1657
MslI CAYNNNNRTG 3 cut(s) 402, 1107, 1314
MspA1I CMGCKG 2 cut(s) 939, 1451
MspI CCGG 8 cut(s) 70, 193, 205, 261, 304, 557, 593, 789
MspR9I CCNGG 5 cut(s) 70, 206, 295, 304, 1028
Mva1269I GAATGC 1 cut(s) 1394
MvaI CCWGG 2 cut(s) 295, 1028
MvnI CGCG 6 cut(s) 16, 138, 155, 172, 278, 550
MwoI GCNNNNNNNGC 5 cut(s) 79, 169, 261, 559, 568
NciI CCSGG 3 cut(s) 70, 206, 304
NcoI CCATGG 1 cut(s) 51
NdeII GATC 8 cut(s) 37, 55, 309, 844, 925, 1015, 1233, 1319
NlaIII CATG 6 cut(s) 55, 401, 1091, 1214, 1262, 1313
NlaIV GGNNCC 9 cut(s) 57, 311, 489, 591, 1168, 1407, 1503, 1556, 1568
NmeAIII GCCGAG 4 cut(s) 271, 487, 516, 912
NmuCI GTSAC 1 cut(s) 904
NotI GCGGCCGC 1 cut(s) 551
NruI TCGCGA 1 cut(s) 278
NsbI TGCGCA 1 cut(s) 425
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 1214, 1313
NspV TTCGAA 1 cut(s) 231
PaeI GCATGC 1 cut(s) 1313
PagI TCATGA 1 cut(s) 1258
PciSI GCTCTTC 1 cut(s) 1074
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 282, 488
PctI GAATGC 1 cut(s) 1394
PdmI GAANNNNTTC 1 cut(s) 874
PfeI GAWTC 5 cut(s) 389, 1127, 1205, 1273, 1573
PflFI GACNNNGTC 1 cut(s) 472
PfoI TCCNGGA 1 cut(s) 302
PleI GAGTC 8 cut(s) 307, 463, 472, 487, 818, 1083, 1437, 1470
PpsI GAGTC 8 cut(s) 307, 463, 472, 487, 818, 1083, 1437, 1470
Psp6I CCWGG 2 cut(s) 293, 1026
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 2 cut(s) 293, 1026
PspN4I GGNNCC 9 cut(s) 57, 311, 489, 591, 1168, 1407, 1503, 1556, 1568
PspPI GGNCC 7 cut(s) 67, 190, 195, 263, 372, 474, 488
PsuI RGATCY 5 cut(s) 55, 309, 844, 1233, 1319
PsyI GACNNNGTC 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 1451
RruI TCGCGA 1 cut(s) 278
RsaI GTAC 4 cut(s) 353, 505, 1193, 1474
RsaNI GTAC 4 cut(s) 352, 504, 1192, 1473
RseI CAYNNNNRTG 3 cut(s) 402, 1107, 1314
Rsr2I CGGWCCG 2 cut(s) 190, 474
RsrII CGGWCCG 2 cut(s) 190, 474
SalI GTCGAC 2 cut(s) 466, 481
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 7 cut(s) 540, 996, 1287, 1419, 1490, 1622, 1657
Sau3AI GATC 8 cut(s) 37, 55, 309, 844, 925, 1015, 1233, 1319
Sau96I GGNCC 7 cut(s) 67, 190, 195, 263, 372, 474, 488
SchI GAGTC 8 cut(s) 308, 463, 473, 488, 818, 1084, 1438, 1471
ScrFI CCNGG 5 cut(s) 70, 206, 295, 304, 1028
SduI GDGCHC 1 cut(s) 1559
SfaNI GCATC 5 cut(s) 13, 397, 599, 971, 1515
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 3 cut(s) 190, 195, 474
SmiMI CAYNNNNRTG 3 cut(s) 402, 1107, 1314
SpeI ACTAGT 1 cut(s) 908
SphI GCATGC 1 cut(s) 1313
Sse9I AATT 4 cut(s) 419, 537, 966, 1542
SspI AATATT 1 cut(s) 856
SspMI CTAG 5 cut(s) 27, 585, 722, 909, 1227
StyD4I CCNGG 5 cut(s) 68, 204, 293, 302, 1026
StyI CCWWGG 2 cut(s) 51, 1384
TaaI ACNGT 6 cut(s) 351, 451, 457, 778, 1477, 1636
TaiI ACGT 2 cut(s) 1193, 1287
TaqI TCGA 9 cut(s) 40, 180, 210, 231, 317, 467, 482, 578, 614
TaqII GACCGA 2 cut(s) 462, 1278
TasI AATT 4 cut(s) 419, 537, 966, 1542
TatI WGTACW 2 cut(s) 503, 1472
TauI GCSGC 7 cut(s) 87, 293, 553, 556, 571, 574, 942
TfiI GAWTC 5 cut(s) 389, 1127, 1205, 1273, 1573
Tru1I TTAA 7 cut(s) 540, 996, 1287, 1419, 1490, 1622, 1657
Tru9I TTAA 7 cut(s) 540, 996, 1287, 1419, 1490, 1622, 1657
TscAI CASTG 1 cut(s) 1647
TseFI GTSAC 1 cut(s) 904
TseI GCWGC 6 cut(s) 30, 79, 157, 160, 936, 1645
Tsp45I GTSAC 1 cut(s) 904
TspDTI ATGAA 9 cut(s) 714, 723, 975, 976, 1091, 1140, 1247, 1379, 1590
TspGWI ACGGA 2 cut(s) 250, 1594
TspRI CASTG 1 cut(s) 1647
Tth111I GACNNNGTC 1 cut(s) 472
VpaK11BI GGWCC 3 cut(s) 190, 195, 474
XapI RAATTY 1 cut(s) 966
XceI RCATGY 2 cut(s) 1214, 1313
XcmI CCANNNNNNNNNTGG 1 cut(s) 1455
XmiI GTMKAC 3 cut(s) 467, 482, 1221
XmnI GAANNNNTTC 1 cut(s) 874
XspI CTAG 5 cut(s) 27, 585, 722, 909, 1227
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.