FvH4_2g34690

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000265
Physical Location & Seq
Forward (+)
25686247 .. 25688945
2699 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34690.t1

Sequence Viewer

Length: 1317 bp
ATGGCGCCATACATGGAGAGAGCACGATCATTCCTTCAGTCTCCCACTTATGGAAACCTCGTCACTGTTCTTAGCATCGACGGAGGTGGAATAAGAGGGATTATTCCCGGGACTATGCTCAGCTTCCTAGAATCCGAGCTTCAGAAGCTGGATGGTGAAGATGCAAGACTGGCAGATTACTTTGATGTTATCGCAGGCACCAGCACAGGTGGTCTTGTCACTGCTATGCTTACTGCCCCAGATGAAAATAACCGTCCCCTATTTGCTGCTAAAGATATTAACGATTTTTATCTTGAACACTGCCCTAAGATGTTCCCCCAACACAGCACCCCACTTATTGGTGAAGCTGTAGAAATGTTAAAAGCTGTGAAAGGGCCAAAATATAATGGGAAGTATTTGCATGATATAGTCAAGGAAAAGCTGGGAGACGTAACATTGAACAAGACATTGACTAACGTCATAATCCCAACTTTTGATATAAAGAGGCTTCAGCCAATCATCTTCTCCAGCTATGAGGTTAAAAAGAAGGATAGCTTAAATGCTTTACTCTCCGACATATGCATTGCAACTTCAGCAGCCCCAACTTATCTCCCAGCACATCATTTTCAAACCAAAGATTGCAGCACCGGAGAAGCAAGAGAATTTGACCTGATTGATGGTGGAGTCGCTGCTAATAATCCGGCCTTGGTCGCTCTAAGCGAAGTAACAAAAGAAATCCATCAAGGAAATCCTGACTTCTTTCCTATAAAACCAAATGAGTATGGGCGATTCCTCGTGATATCACTGGGTACTGGTTCGGCGAAATGCGAGGAGAAGTATGAGGCCACAAATGCAGGTAGGTGGGGCGTTTTGGGGTGGCTAACCGGAGGCGGTTCATGTCCACTGGTTGACGTTTTTACTCAAGCCAGTAGTGACATGGTTGACTATCACCTCTCTACGGTCTTCCAAGCTCTTCATTCTGAGAAAAACTACCTTCGGATTCAAGACGATACGCTGGAGAAAACAGTGTGTTCAGTGGACATTGCTACCCATGAAAACTTGAACAATCTTGTAAAAGTTGGAGAGGAATTGTTAAAGAAACCGGTTTCCAGGGTGAACATGGAAACTGGTATGTTTGAGCCTGCCCATCAGTCCACAAATGAAGAGGCTCTCGTAAGGGTAGCAGGAATTCTGTCTAGAGAGAGGAGGGTTCGTGAAATGAGATCACCCCATGGGAAGGCTGCAGTCGCTGCAGCTAGGGCTGGCAAATTGGGCCATGTTGAAATTAACCAACCGAGTACCAACCGTACCAACGTAGTTAGTATACCAAAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

439

Amino Acids

47.97

Weight (kDa)

6.03

Isoelectric Point (pI)

31.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 24 - 232 8e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 824
AccB1I GGYRCC 2 cut(s) 4, 197
AccB7I CCANNNNNTGG 1 cut(s) 338
AccI GTMKAC 1 cut(s) 1303
AciI CCGC 1 cut(s) 870
AcsI RAATTY 2 cut(s) 641, 1167
AcuI CTGAAG 4 cut(s) 20, 125, 473, 555
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 790, 1279, 1288
AfiI CCNNNNNNNGG 4 cut(s) 50, 338, 937, 1216
AgeI ACCGGT 1 cut(s) 1081
AgsI TTSAA 6 cut(s) 296, 439, 608, 983, 1042, 1262
AjnI CCWGG 1 cut(s) 1088
Alw21I GWGCWC 1 cut(s) 25
Alw26I GTCTC 2 cut(s) 45, 420
AlwNI CAGNNNCTG 2 cut(s) 148, 1229
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 4 cut(s) 374, 681, 822, 1252
ApeKI GCWGC 7 cut(s) 266, 575, 621, 668, 1220, 1229, 1232
ApoI RAATTY 2 cut(s) 641, 1167
AsiGI ACCGGT 1 cut(s) 1081
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 374, 1252
AsuC2I CCSGG 2 cut(s) 108, 109
AsuHPI GGTGA 5 cut(s) 167, 353, 920, 1105, 1197
AvaI CYCGRG 1 cut(s) 107
BanI GGYRCC 2 cut(s) 4, 197
BauI CACGAG 1 cut(s) 773
BbsI GAAGAC 1 cut(s) 934
Bbv12I GWGCWC 1 cut(s) 25
BbvI GCAGC 7 cut(s) 253, 587, 633, 655, 1207, 1216, 1244
BccI CCATC 4 cut(s) 146, 650, 726, 1134
BciT130I CCWGG 1 cut(s) 1090
BcnI CCSGG 2 cut(s) 108, 109
BcoDI GTCTC 2 cut(s) 45, 420
BfaI CTAG 3 cut(s) 128, 1176, 1236
BfmI CTRYAG 3 cut(s) 348, 1221, 1230
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 824
BisI GCNGC 7 cut(s) 267, 576, 622, 669, 1221, 1230, 1233
BlpI GCTNAGC 1 cut(s) 119
BlsI GCNGC 7 cut(s) 268, 577, 623, 670, 1222, 1231, 1234
Bme1390I CCNGG 3 cut(s) 108, 109, 1090
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 2 cut(s) 374, 1252
BmiI GGNNCC 2 cut(s) 6, 199
BmrFI CCNGG 3 cut(s) 108, 109, 1090
BmrI ACTGGG 1 cut(s) 794
BmsI GCATC 2 cut(s) 84, 151
BmuI ACTGGG 1 cut(s) 794
BoxI GACNNNNGTC 1 cut(s) 455
BpiI GAAGAC 1 cut(s) 934
BpmI CTGGAG 2 cut(s) 490, 1016
Bpu1102I GCTNAGC 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 885
BpuMI CCSGG 2 cut(s) 108, 109
BsaBI GATNNNNATC 1 cut(s) 288
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 107, 684, 1089, 1210
BsaWI WCCGGW 3 cut(s) 626, 863, 1081
BsaXI ACNNNNNCTCC 2 cut(s) 1177, 1207
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 338, 937, 1216
Bse118I RCCGGY 1 cut(s) 1081
Bse1I ACTGG 6 cut(s) 174, 789, 796, 888, 906, 1111
Bse3DI GCAATG 2 cut(s) 561, 1020
Bse8I GATNNNNATC 1 cut(s) 288
BseBI CCWGG 1 cut(s) 1090
BseDI CCNNGG 4 cut(s) 107, 684, 1089, 1210
BseGI GGATG 1 cut(s) 157
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 4 cut(s) 50, 338, 937, 1216
BseMI GCAATG 2 cut(s) 561, 1020
BseMII CTCAG 2 cut(s) 133, 951
BseNI ACTGG 6 cut(s) 174, 789, 796, 888, 906, 1111
BseRI GAGGAG 2 cut(s) 824, 1198
BseXI GCAGC 7 cut(s) 253, 587, 633, 655, 1207, 1216, 1244
BseYI CCCAGC 2 cut(s) 421, 592
BshFI GGCC 4 cut(s) 376, 683, 824, 1254
BshNI GGYRCC 2 cut(s) 4, 197
BshTI ACCGGT 1 cut(s) 1081
BsiHKAI GWGCWC 1 cut(s) 25
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 5 cut(s) 108, 627, 680, 864, 1082
BslFI GGGAC 2 cut(s) 124, 240
BslI CCNNNNNNNGG 4 cut(s) 50, 338, 937, 1216
BsmAI GTCTC 2 cut(s) 45, 420
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 2 cut(s) 124, 240
BsnI GGCC 4 cut(s) 376, 683, 824, 1254
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 25
Bsp143I GATC 2 cut(s) 26, 1202
Bsp1720I GCTNAGC 1 cut(s) 119
Bsp19I CCATGG 1 cut(s) 1210
BspACI CCGC 1 cut(s) 870
BspANI GGCC 4 cut(s) 376, 683, 824, 1254
BspCNI CTCAG 2 cut(s) 132, 952
BspLI GGNNCC 2 cut(s) 6, 199
BspMAI CTGCAG 2 cut(s) 1225, 1234
BspMI ACCTGC 1 cut(s) 824
BspQI GCTCTTC 1 cut(s) 957
BspT107I GGYRCC 2 cut(s) 4, 197
BsrDI GCAATG 2 cut(s) 561, 1020
BsrFI RCCGGY 1 cut(s) 1081
BsrI ACTGG 6 cut(s) 174, 789, 796, 888, 906, 1111
BssAI RCCGGY 1 cut(s) 1081
BssECI CCNNGG 4 cut(s) 107, 684, 1089, 1210
BssMI GATC 2 cut(s) 26, 1202
BssNAI GTATAC 1 cut(s) 1304
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 773
BssT1I CCWWGG 2 cut(s) 684, 1210
Bst1107I GTATAC 1 cut(s) 1304
Bst2BI CACGAG 1 cut(s) 773
Bst2UI CCWGG 1 cut(s) 1090
Bst4CI ACNGT 5 cut(s) 67, 254, 940, 1006, 1286
Bst6I CTCTTC 2 cut(s) 957, 1137
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1229
BstC8I GCNNGC 3 cut(s) 196, 1122, 1243
BstDEI CTNAG 5 cut(s) 71, 119, 306, 695, 960
BstDSI CCRYGG 1 cut(s) 1210
BstF5I GGATG 1 cut(s) 157
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 29, 1205
BstMAI GTCTC 2 cut(s) 45, 420
BstMBI GATC 2 cut(s) 26, 1202
BstMWI GCNNNNNNNGC 9 cut(s) 145, 170, 572, 689, 830, 1226, 1229, 1238, 1251
BstNI CCWGG 1 cut(s) 1090
BstPAI GACNNNNGTC 1 cut(s) 455
BstSCI CCNGG 3 cut(s) 106, 107, 1088
BstSFI CTRYAG 3 cut(s) 348, 1221, 1230
BstV1I GCAGC 7 cut(s) 253, 587, 633, 655, 1207, 1216, 1244
BstV2I GAAGAC 1 cut(s) 934
BstZ17I GTATAC 1 cut(s) 1304
BsuRI GGCC 4 cut(s) 376, 683, 824, 1254
BtgI CCRYGG 1 cut(s) 1210
BtsCI GGATG 1 cut(s) 157
BtsI GCAGTG 2 cut(s) 219, 298
BtsIMutI CAGTG 7 cut(s) 63, 219, 298, 782, 881, 1011, 1020
BveI ACCTGC 1 cut(s) 824
Cac8I GCNNGC 3 cut(s) 196, 1122, 1243
CaiI CAGNNNCTG 2 cut(s) 148, 1229
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 1081
Cfr13I GGNCC 2 cut(s) 374, 1252
Cfr9I CCCGGG 1 cut(s) 107
Csp6I GTAC 3 cut(s) 789, 1278, 1287
CspAI ACCGGT 1 cut(s) 1081
CviAII CATG 8 cut(s) 13, 401, 876, 916, 1031, 1099, 1211, 1256
CviQI GTAC 3 cut(s) 789, 1278, 1287
DdeI CTNAG 5 cut(s) 71, 119, 306, 695, 960
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 28, 1204
DpnII GATC 2 cut(s) 26, 1202
Eam1104I CTCTTC 2 cut(s) 957, 1137
EarI CTCTTC 2 cut(s) 957, 1137
Eco130I CCWWGG 2 cut(s) 684, 1210
Eco32I GATATC 1 cut(s) 780
Eco57I CTGAAG 4 cut(s) 20, 125, 473, 555
Eco88I CYCGRG 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 1167
EcoRII CCWGG 1 cut(s) 1088
EcoRV GATATC 1 cut(s) 780
EcoT14I CCWWGG 2 cut(s) 684, 1210
EcoT22I ATGCAT 1 cut(s) 563
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 684, 1210
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 8 cut(s) 16, 404, 879, 919, 1034, 1102, 1214, 1259
FalI AAGNNNNNCTT 2 cut(s) 518, 550
FaqI GGGAC 2 cut(s) 124, 240
FatI CATG 8 cut(s) 12, 400, 875, 915, 1030, 1098, 1210, 1255
FauNDI CATATG 1 cut(s) 557
FblI GTMKAC 1 cut(s) 1303
Fnu4HI GCNGC 7 cut(s) 267, 576, 622, 669, 1221, 1230, 1233
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 7 cut(s) 267, 576, 622, 669, 1221, 1230, 1233
FspBI CTAG 3 cut(s) 128, 1176, 1236
GlaI GCGC 1 cut(s) 6
GluI GCNGC 7 cut(s) 267, 576, 622, 669, 1221, 1230, 1233
GsaI CCCAGC 2 cut(s) 425, 596
GsuI CTGGAG 2 cut(s) 490, 1016
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 376, 683, 824, 1254
HapII CCGG 5 cut(s) 108, 627, 680, 864, 1082
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 16, 404, 879, 919, 1034, 1102, 1214, 1259
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 889, 922
HindII GTYRAC 2 cut(s) 889, 922
HinfI GANTC 4 cut(s) 131, 663, 768, 979
HpaII CCGG 5 cut(s) 108, 627, 680, 864, 1082
HphI GGTGA 5 cut(s) 167, 353, 920, 1105, 1197
Hpy166II GTNNAC 7 cut(s) 881, 889, 922, 1018, 1096, 1134, 1304
Hpy188I TCNGA 5 cut(s) 136, 144, 553, 961, 978
Hpy188III TCNNGA 6 cut(s) 293, 731, 775, 983, 1176, 1193
Hpy8I GTNNAC 7 cut(s) 881, 889, 922, 1018, 1096, 1134, 1304
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 4 cut(s) 44, 520, 983, 1210
HpyCH4III ACNGT 5 cut(s) 67, 254, 940, 1006, 1286
HpyCH4IV ACGT 4 cut(s) 429, 456, 891, 1293
HpyCH4V TGCA 8 cut(s) 164, 400, 561, 566, 621, 833, 1223, 1232
HpyF10VI GCNNNNNNNGC 9 cut(s) 145, 170, 572, 689, 830, 1226, 1229, 1238, 1251
HpyF3I CTNAG 5 cut(s) 71, 119, 306, 695, 960
HpySE526I ACGT 4 cut(s) 429, 456, 891, 1293
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 16, 404, 879, 919, 1034, 1102, 1214, 1259
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 26, 1202
LguI GCTCTTC 1 cut(s) 957
Lsp1109I GCAGC 7 cut(s) 253, 587, 633, 655, 1207, 1216, 1244
LweI GCATC 2 cut(s) 84, 151
MaeI CTAG 3 cut(s) 128, 1176, 1236
MaeII ACGT 4 cut(s) 429, 456, 891, 1293
MaeIII GTNAC 5 cut(s) 61, 217, 430, 703, 911
MalI GATC 2 cut(s) 28, 1204
MboI GATC 2 cut(s) 26, 1202
MboII GAAGA 5 cut(s) 170, 493, 934, 944, 1154
MhlI GDGCHC 1 cut(s) 25
MluCI AATT 5 cut(s) 641, 1067, 1167, 1247, 1263
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 672
MmeI TCCRAC 2 cut(s) 576, 1039
Mph1103I ATGCAT 1 cut(s) 563
MseI TTAA 6 cut(s) 279, 359, 519, 536, 1073, 1266
MslI CAYNNNNRTG 1 cut(s) 224
MspI CCGG 5 cut(s) 108, 627, 680, 864, 1082
MspR9I CCNGG 3 cut(s) 108, 109, 1090
MvaI CCWGG 1 cut(s) 1090
MwoI GCNNNNNNNGC 9 cut(s) 145, 170, 572, 689, 830, 1226, 1229, 1238, 1251
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 108, 109
NcoI CCATGG 1 cut(s) 1210
NdeI CATATG 1 cut(s) 557
NdeII GATC 2 cut(s) 26, 1202
NlaIII CATG 8 cut(s) 16, 404, 879, 919, 1034, 1102, 1214, 1259
NlaIV GGNNCC 2 cut(s) 6, 199
NmuCI GTSAC 3 cut(s) 61, 217, 911
NsiI ATGCAT 1 cut(s) 563
PciSI GCTCTTC 1 cut(s) 957
PcsI WCGNNNNNNNCGW 1 cut(s) 696
PfeI GAWTC 3 cut(s) 131, 768, 979
PflMI CCANNNNNTGG 1 cut(s) 338
PinAI ACCGGT 1 cut(s) 1081
PkrI GCNGC 7 cut(s) 268, 577, 623, 670, 1222, 1231, 1234
PleI GAGTC 1 cut(s) 671
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 671
PshAI GACNNNNGTC 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 1088
PspFI CCCAGC 2 cut(s) 421, 592
PspGI CCWGG 1 cut(s) 1088
PspN4I GGNNCC 2 cut(s) 6, 199
PspPI GGNCC 2 cut(s) 374, 1252
PstI CTGCAG 2 cut(s) 1225, 1234
PstNI CAGNNNCTG 2 cut(s) 148, 1229
RsaI GTAC 3 cut(s) 790, 1279, 1288
RsaNI GTAC 3 cut(s) 789, 1278, 1287
RseI CAYNNNNRTG 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 957
SaqAI TTAA 6 cut(s) 279, 359, 519, 536, 1073, 1266
SatI GCNGC 7 cut(s) 267, 576, 622, 669, 1221, 1230, 1233
Sau3AI GATC 2 cut(s) 26, 1202
Sau96I GGNCC 2 cut(s) 374, 1252
SchI GAGTC 1 cut(s) 672
ScrFI CCNGG 3 cut(s) 108, 109, 1090
SduI GDGCHC 1 cut(s) 25
SfaNI GCATC 2 cut(s) 84, 151
SfcI CTRYAG 3 cut(s) 348, 1221, 1230
SfoI GGCGCC 1 cut(s) 6
SmaI CCCGGG 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 900
SmoI CTYRAG 1 cut(s) 900
Sse9I AATT 5 cut(s) 641, 1067, 1167, 1247, 1263
SsiI CCGC 1 cut(s) 870
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 128, 1176, 1236
StyD4I CCNGG 3 cut(s) 106, 107, 1088
StyI CCWWGG 2 cut(s) 684, 1210
TaaI ACNGT 5 cut(s) 67, 254, 940, 1006, 1286
TaiI ACGT 4 cut(s) 432, 459, 894, 1296
TaqI TCGA 1 cut(s) 78
TasI AATT 5 cut(s) 641, 1067, 1167, 1247, 1263
TfiI GAWTC 3 cut(s) 131, 768, 979
Tru1I TTAA 6 cut(s) 279, 359, 519, 536, 1073, 1266
Tru9I TTAA 6 cut(s) 279, 359, 519, 536, 1073, 1266
TscAI CASTG 7 cut(s) 70, 226, 305, 789, 888, 1011, 1020
TseFI GTSAC 3 cut(s) 61, 217, 911
TseI GCWGC 7 cut(s) 266, 575, 621, 668, 1220, 1229, 1232
Tsp45I GTSAC 3 cut(s) 61, 217, 911
TspDTI ATGAA 5 cut(s) 258, 864, 944, 1047, 1155
TspGWI ACGGA 1 cut(s) 96
TspMI CCCGGG 1 cut(s) 107
TspRI CASTG 7 cut(s) 70, 226, 305, 789, 888, 1011, 1020
Van91I CCANNNNNTGG 1 cut(s) 338
XapI RAATTY 2 cut(s) 641, 1167
XbaI TCTAGA 1 cut(s) 1175
XcmI CCANNNNNNNNNTGG 2 cut(s) 913, 1096
XmaI CCCGGG 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 1303
XspI CTAG 3 cut(s) 128, 1176, 1236
Zsp2I ATGCAT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.