pycom08g08680

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000265
Physical Location & Seq
Reverse (-)
6988227 .. 6989323
1097 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08680.4

Sequence Viewer

Length: 705 bp
ATGGAAAGAAGCGGTAGAGTAGAGCCCCCCACAGTCTTTGGAAAGATAGTCACAGTTCTCAGTATCGATGGCGGCGGAATAAGAGGCCTCATTCCGGCAACCATTCTTGCTTTCCTCGAATCTGAACTTCAGAAACTGGATGGTGAAGATGCAAGGATCGCCGACTATTTTGATGTGATTGCAGGAACAAGCACAGGTGGTCTTGTGACTGCTATGCTTGCAGCCCCAAACGAGAATAACCGGCCACTGTTCGCTGCCAAAGATATTATAGACTTCTATATTAATCAATGCCCTAAAATCTTCCCCCAAAACACTTGGCCAATTTTTCCTAATACGACAAAGATCATCAAAGCTCTAGCAGGACCAAAATACAATGGCAAGTATTTGCATCGCTTGATTAGGGAAAGGCTGGGCAACAAAAAACTACACGACACATTGACTAATGTTGTAATTCCCACATTTGACATCAAGAATCTCCAGCCAGCTATCTTCTGCAACTTTAACGTGAAAAAGAAGCCATCATACGATGCCTTACTCTCTGACATATGCATTGGAACCTCAGCAGCACCAACTTATCTTCCAGCTCATTATTTTGAAACCAAGAACGCTGCAGGCAGAACTAGGGAATTCAACCTTATAGATGGCGGCGTGGCTGCAAATAATCCGGTATATGTGATAGGAAGAGTTATATTACCAGATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

235

Amino Acids

25.66

Weight (kDa)

8.82

Isoelectric Point (pI)

27.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 12, 72, 75, 645
AclWI GGATC 1 cut(s) 164
AcoI YGGCCR 2 cut(s) 242, 317
AcsI RAATTY 1 cut(s) 626
AcuI CTGAAG 1 cut(s) 113
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 2 cut(s) 596, 631
AluBI AGCT 3 cut(s) 353, 485, 584
AluI AGCT 3 cut(s) 353, 485, 584
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 136
AoxI GGCC 3 cut(s) 85, 242, 317
ApeKI GCWGC 5 cut(s) 221, 254, 563, 608, 653
ApoI RAATTY 1 cut(s) 626
AseI ATTAAT 1 cut(s) 282
AspS9I GGNCC 1 cut(s) 362
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 319
BanII GRGCYC 1 cut(s) 27
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BbvCI CCTCAGC 1 cut(s) 559
BbvI GCAGC 5 cut(s) 233, 241, 575, 595, 640
BccI CCATC 4 cut(s) 62, 134, 526, 635
BfaI CTAG 2 cut(s) 356, 621
BfmI CTRYAG 1 cut(s) 609
BisI GCNGC 7 cut(s) 73, 222, 255, 564, 609, 646, 654
BlsI GCNGC 7 cut(s) 74, 223, 256, 565, 610, 647, 655
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 362
BmiI GGNNCC 1 cut(s) 556
BmsI GCATC 3 cut(s) 139, 397, 517
BpmI CTGGAG 1 cut(s) 461
Bpu10I CCTNAGC 1 cut(s) 559
Bsa29I ATCGAT 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 141
BseCI ATCGAT 1 cut(s) 66
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMII CTCAG 2 cut(s) 73, 573
BseNI ACTGG 1 cut(s) 141
BseXI GCAGC 5 cut(s) 233, 241, 575, 595, 640
BseYI CCCAGC 1 cut(s) 409
BshFI GGCC 3 cut(s) 87, 244, 319
BshVI ATCGAT 1 cut(s) 66
BsiSI CCGG 3 cut(s) 95, 241, 665
BslI CCNNNNNNNGG 1 cut(s) 94
BsnI GGCC 3 cut(s) 87, 244, 319
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 156, 342
BspACI CCGC 4 cut(s) 12, 72, 75, 645
BspANI GGCC 3 cut(s) 87, 244, 319
BspCNI CTCAG 2 cut(s) 72, 572
BspDI ATCGAT 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 556
BspMAI CTGCAG 1 cut(s) 613
BspPI GGATC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 240
BsrI ACTGG 1 cut(s) 141
BssAI RCCGGY 1 cut(s) 240
BssMI GATC 2 cut(s) 156, 342
Bst4CI ACNGT 3 cut(s) 34, 55, 249
Bst6I CTCTTC 1 cut(s) 676
BstC8I GCNNGC 3 cut(s) 219, 483, 613
BstDEI CTNAG 2 cut(s) 59, 559
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 2 cut(s) 159, 345
BstMBI GATC 2 cut(s) 156, 342
BstMWI GCNNNNNNNGC 2 cut(s) 158, 218
BstSFI CTRYAG 1 cut(s) 609
BstV1I GCAGC 5 cut(s) 233, 241, 575, 595, 640
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 3 cut(s) 87, 244, 319
BsuTUI ATCGAT 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 374
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 3 cut(s) 219, 483, 613
CaiI CAGNNNCTG 1 cut(s) 136
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 362
ClaI ATCGAT 1 cut(s) 66
CspCI CAANNNNNGTGG 2 cut(s) 19, 54
DdeI CTNAG 2 cut(s) 59, 559
DpnI GATC 2 cut(s) 158, 344
DpnII GATC 2 cut(s) 156, 342
EaeI YGGCCR 2 cut(s) 242, 317
Eam1104I CTCTTC 1 cut(s) 676
EarI CTCTTC 1 cut(s) 676
EciI GGCGGA 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 362
Eco57I CTGAAG 1 cut(s) 113
EcoRI GAATTC 1 cut(s) 626
EcoT22I ATGCAT 1 cut(s) 551
EcoT38I GRGCYC 1 cut(s) 27
FauNDI CATATG 1 cut(s) 545
Fnu4HI GCNGC 7 cut(s) 73, 222, 255, 564, 609, 646, 654
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 7 cut(s) 73, 222, 255, 564, 609, 646, 654
FspBI CTAG 2 cut(s) 356, 621
GluI GCNGC 7 cut(s) 73, 222, 255, 564, 609, 646, 654
GsaI CCCAGC 1 cut(s) 413
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 3 cut(s) 87, 244, 319
HapII CCGG 3 cut(s) 95, 241, 665
HinfI GANTC 2 cut(s) 119, 472
HpaII CCGG 3 cut(s) 95, 241, 665
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 3 cut(s) 124, 132, 541
Hpy188III TCNNGA 1 cut(s) 469
HpyCH4III ACNGT 3 cut(s) 34, 55, 249
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 8 cut(s) 152, 182, 221, 388, 495, 549, 611, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 218
HpyF3I CTNAG 2 cut(s) 59, 559
HpySE526I ACGT 1 cut(s) 504
Kzo9I GATC 2 cut(s) 156, 342
Lsp1109I GCAGC 5 cut(s) 233, 241, 575, 595, 640
LweI GCATC 3 cut(s) 139, 397, 517
MaeI CTAG 2 cut(s) 356, 621
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 2 cut(s) 49, 205
MalI GATC 2 cut(s) 158, 344
MboI GATC 2 cut(s) 156, 342
MboII GAAGA 5 cut(s) 158, 292, 481, 569, 693
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 1 cut(s) 319
MluCI AATT 3 cut(s) 321, 450, 626
MluNI TGGCCA 1 cut(s) 319
MnlI CCTC 4 cut(s) 77, 98, 125, 568
Mox20I TGGCCA 1 cut(s) 319
Mph1103I ATGCAT 1 cut(s) 551
MscI TGGCCA 1 cut(s) 319
MseI TTAA 3 cut(s) 282, 501, 703
Msp20I TGGCCA 1 cut(s) 319
MspI CCGG 3 cut(s) 95, 241, 665
MwoI GCNNNNNNNGC 2 cut(s) 158, 218
NdeI CATATG 1 cut(s) 545
NdeII GATC 2 cut(s) 156, 342
NlaIV GGNNCC 1 cut(s) 556
NmuCI GTSAC 2 cut(s) 49, 205
NsiI ATGCAT 1 cut(s) 551
PceI AGGCCT 1 cut(s) 87
PfeI GAWTC 2 cut(s) 119, 472
PkrI GCNGC 7 cut(s) 74, 223, 256, 565, 610, 647, 655
PshBI ATTAAT 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 1 cut(s) 362
PstI CTGCAG 1 cut(s) 613
PstNI CAGNNNCTG 1 cut(s) 136
SaqAI TTAA 3 cut(s) 282, 501, 703
SatI GCNGC 7 cut(s) 73, 222, 255, 564, 609, 646, 654
Sau3AI GATC 2 cut(s) 156, 342
Sau96I GGNCC 1 cut(s) 362
SduI GDGCHC 1 cut(s) 27
SetI ASST 7 cut(s) 199, 355, 487, 507, 560, 586, 636
SfaNI GCATC 3 cut(s) 139, 397, 517
SfcI CTRYAG 1 cut(s) 609
SinI GGWCC 1 cut(s) 362
Sse9I AATT 3 cut(s) 321, 450, 626
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 4 cut(s) 12, 72, 75, 645
SspMI CTAG 2 cut(s) 356, 621
StuI AGGCCT 1 cut(s) 87
TaaI ACNGT 3 cut(s) 34, 55, 249
TaiI ACGT 1 cut(s) 507
TaqI TCGA 2 cut(s) 66, 117
TasI AATT 3 cut(s) 321, 450, 626
TauI GCSGC 2 cut(s) 75, 648
TfiI GAWTC 2 cut(s) 119, 472
Tru1I TTAA 3 cut(s) 282, 501, 703
Tru9I TTAA 3 cut(s) 282, 501, 703
TscAI CASTG 1 cut(s) 252
TseFI GTSAC 2 cut(s) 49, 205
TseI GCWGC 5 cut(s) 221, 254, 563, 608, 653
Tsp45I GTSAC 2 cut(s) 49, 205
TspRI CASTG 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 362
VspI ATTAAT 1 cut(s) 282
XapI RAATTY 1 cut(s) 626
XspI CTAG 2 cut(s) 356, 621
Zsp2I ATGCAT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.