Rroxscaffold_7G00161060

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000265
Physical Location & Seq
Reverse (-)
3900579 .. 3907274
6696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161060.1

Sequence Viewer

Length: 663 bp
ATGAGCGATAACGTGATCAACCATGGTGGAAGAGAGGAGGATGCCGAGAGGAGAAGATGCGAAGAAACCCTAGCCGAAACCCTAACGAGTGAAAAAAACCCTAGCACCGCACCAACGACTTATCTTCCAGCTCATTATTTTGAAACCAACGGCCTAAAAGGAAAAGTTAGAGAATTTCACCTGATAGATGGTGGATTGGCAGCAAATAATCTGGATGGAAATAAACAAACGCAGGATCACTCACTAAGTGGCGAAGTATCTTCGGTAGATATAGCAATAGAGAAGAATCTGGACCATCTTCTCAAAGTTGGTGAAGGACTATTGAGACAACCAGTTTCCGGGGTTAATTTAGAGACATGCATATTCGAAGCTTGTAATCATGAGACTAATAAAGAGGCTCTTATAAGATTTGCAAAATTGCTGTCCGAAGAGAAGTGGCTTCGCCTTTCAAGGTCTCCCCATGGACGTACATATTGCAAAGTCTCACCGATATGTATGTTACAAAGTTCCACGAGAAAGTCTTGGCATATGAGATCGAGTATCAAGCTTATCGATCAGCTGGTAGATGTTGGGTTTGCTTGTGGTGCTGCTGATCGATTGCACGTTCCTTCTTCTCTTTGGAGAGAATTGCCGCTACGTACCCTGCATATGCAGAATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

220

Amino Acids

24.69

Weight (kDa)

6.37

Isoelectric Point (pI)

56.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 404
AciI CCGC 2 cut(s) 108, 632
AclWI GGATC 1 cut(s) 243
AcsI RAATTY 1 cut(s) 173
AdeI CACNNNGTG 1 cut(s) 248
AfaI GTAC 2 cut(s) 469, 640
AfiI CCNNNNNNNGG 1 cut(s) 338
AgsI TTSAA 2 cut(s) 143, 450
AluBI AGCT 4 cut(s) 131, 371, 547, 559
AluI AGCT 4 cut(s) 131, 371, 547, 559
Alw26I GTCTC 5 cut(s) 319, 347, 377, 459, 487
AlwI GGATC 1 cut(s) 243
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 2 cut(s) 200, 587
ApoI RAATTY 1 cut(s) 173
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AspS9I GGNCC 1 cut(s) 292
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 3 cut(s) 170, 323, 477
AsuII TTCGAA 1 cut(s) 366
AvaII GGWCC 1 cut(s) 292
BauI CACGAG 1 cut(s) 511
BbvI GCAGC 2 cut(s) 212, 574
BccI CCATC 3 cut(s) 182, 209, 303
BceAI ACGGC 1 cut(s) 166
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 5 cut(s) 319, 347, 377, 459, 487
BfaI CTAG 2 cut(s) 71, 102
BisI GCNGC 3 cut(s) 201, 588, 632
BlsI GCNGC 3 cut(s) 202, 589, 633
Bme1390I CCNGG 1 cut(s) 340
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 292
BmrFI CCNGG 1 cut(s) 340
BmsI GCATC 2 cut(s) 31, 47
Bpu14I TTCGAA 1 cut(s) 366
BpuMI CCSGG 1 cut(s) 340
Bsa29I ATCGAT 2 cut(s) 552, 595
BsaAI YACGTR 1 cut(s) 638
BsaI GGTCTC 1 cut(s) 459
BsaJI CCNNGG 3 cut(s) 22, 339, 460
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 1 cut(s) 332
BseCI ATCGAT 2 cut(s) 552, 595
BseDI CCNNGG 3 cut(s) 22, 339, 460
BseGI GGATG 2 cut(s) 46, 220
BseLI CCNNNNNNNGG 1 cut(s) 338
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 2 cut(s) 50, 64
BseXI GCAGC 2 cut(s) 212, 574
BshFI GGCC 1 cut(s) 153
BshVI ATCGAT 2 cut(s) 552, 595
BsiSI CCGG 1 cut(s) 339
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 5 cut(s) 319, 347, 377, 459, 487
BsnI GGCC 1 cut(s) 153
Bso31I GGTCTC 1 cut(s) 459
Bsp119I TTCGAA 1 cut(s) 366
Bsp143I GATC 5 cut(s) 15, 235, 533, 553, 592
Bsp19I CCATGG 2 cut(s) 22, 460
BspACI CCGC 2 cut(s) 108, 632
BspANI GGCC 1 cut(s) 153
BspDI ATCGAT 2 cut(s) 552, 595
BspHI TCATGA 1 cut(s) 379
BspPI GGATC 1 cut(s) 243
BspT104I TTCGAA 1 cut(s) 366
BspTNI GGTCTC 1 cut(s) 459
BsrI ACTGG 1 cut(s) 332
BssECI CCNNGG 3 cut(s) 22, 339, 460
BssMI GATC 5 cut(s) 15, 235, 533, 553, 592
BssSI CACGAG 1 cut(s) 511
BssT1I CCWWGG 2 cut(s) 22, 460
Bst2BI CACGAG 1 cut(s) 511
Bst6I CTCTTC 2 cut(s) 25, 423
BstBAI YACGTR 1 cut(s) 638
BstBI TTCGAA 1 cut(s) 366
BstDEI CTNAG 1 cut(s) 245
BstDSI CCRYGG 2 cut(s) 22, 460
BstF5I GGATG 2 cut(s) 46, 220
BstKTI GATC 5 cut(s) 18, 238, 536, 556, 595
BstMAI GTCTC 5 cut(s) 319, 347, 377, 459, 487
BstMBI GATC 5 cut(s) 15, 235, 533, 553, 592
BstMWI GCNNNNNNNGC 1 cut(s) 584
BstNSI RCATGY 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 338
BstSNI TACGTA 1 cut(s) 638
BstV1I GCAGC 2 cut(s) 212, 574
Bsu15I ATCGAT 2 cut(s) 552, 595
BsuRI GGCC 1 cut(s) 153
BsuTUI ATCGAT 2 cut(s) 552, 595
BtgI CCRYGG 2 cut(s) 22, 460
BtsCI GGATG 2 cut(s) 46, 220
CciI TCATGA 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 292
ClaI ATCGAT 2 cut(s) 552, 595
Csp6I GTAC 2 cut(s) 468, 639
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 4 cut(s) 23, 357, 380, 461
CviJI RGCY 8 cut(s) 74, 131, 153, 371, 398, 439, 547, 559
CviKI_1 RGCY 8 cut(s) 74, 131, 153, 371, 398, 439, 547, 559
CviQI GTAC 2 cut(s) 468, 639
DdeI CTNAG 1 cut(s) 245
DpnI GATC 5 cut(s) 17, 237, 535, 555, 594
DpnII GATC 5 cut(s) 15, 235, 533, 553, 592
DraIII CACNNNGTG 1 cut(s) 248
Eam1104I CTCTTC 2 cut(s) 25, 423
EarI CTCTTC 2 cut(s) 25, 423
Eco105I TACGTA 1 cut(s) 638
Eco130I CCWWGG 2 cut(s) 22, 460
Eco31I GGTCTC 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 292
EcoT14I CCWWGG 2 cut(s) 22, 460
EcoT22I ATGCAT 1 cut(s) 362
ErhI CCWWGG 2 cut(s) 22, 460
FaeI CATG 4 cut(s) 26, 360, 383, 464
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 4 cut(s) 22, 356, 379, 460
FauNDI CATATG 2 cut(s) 528, 648
FbaI TGATCA 1 cut(s) 15
Fnu4HI GCNGC 3 cut(s) 201, 588, 632
FokI GGATG 2 cut(s) 53, 227
Fsp4HI GCNGC 3 cut(s) 201, 588, 632
FspBI CTAG 2 cut(s) 71, 102
GluI GCNGC 3 cut(s) 201, 588, 632
HaeIII GGCC 1 cut(s) 153
HapII CCGG 1 cut(s) 339
Hin1II CATG 4 cut(s) 26, 360, 383, 464
HindIII AAGCTT 2 cut(s) 369, 545
HinfI GANTC 1 cut(s) 286
HpaII CCGG 1 cut(s) 339
HphI GGTGA 3 cut(s) 170, 323, 477
Hpy188I TCNGA 1 cut(s) 427
Hpy188III TCNNGA 3 cut(s) 212, 290, 380
HpyAV CCTTC 2 cut(s) 308, 618
HpyCH4IV ACGT 4 cut(s) 12, 466, 603, 637
HpyCH4V TGCA 6 cut(s) 360, 413, 477, 601, 646, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 584
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 4 cut(s) 12, 466, 603, 637
Hsp92II CATG 4 cut(s) 26, 360, 383, 464
Ksp22I TGATCA 1 cut(s) 15
Kzo9I GATC 5 cut(s) 15, 235, 533, 553, 592
LpnPI CCDG 9 cut(s) 141, 194, 197, 218, 275, 345, 352, 545, 656
Lsp1109I GCAGC 2 cut(s) 212, 574
LweI GCATC 2 cut(s) 31, 47
MaeI CTAG 2 cut(s) 71, 102
MaeII ACGT 4 cut(s) 12, 466, 603, 637
MaeIII GTNAC 1 cut(s) 498
MalI GATC 5 cut(s) 17, 237, 535, 555, 594
MboI GATC 5 cut(s) 15, 235, 533, 553, 592
MboII GAAGA 9 cut(s) 42, 66, 74, 116, 252, 290, 295, 440, 603
MluCI AATT 4 cut(s) 173, 346, 416, 626
MnlI CCTC 4 cut(s) 28, 31, 42, 388
Mph1103I ATGCAT 1 cut(s) 362
MseI TTAA 2 cut(s) 345, 661
MslI CAYNNNNRTG 1 cut(s) 490
MspA1I CMGCKG 1 cut(s) 559
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 1 cut(s) 340
MwoI GCNNNNNNNGC 1 cut(s) 584
NciI CCSGG 1 cut(s) 340
NcoI CCATGG 2 cut(s) 22, 460
NdeI CATATG 2 cut(s) 528, 648
NdeII GATC 5 cut(s) 15, 235, 533, 553, 592
NlaIII CATG 4 cut(s) 26, 360, 383, 464
NmeAIII GCCGAG 1 cut(s) 70
NsiI ATGCAT 1 cut(s) 362
NspI RCATGY 1 cut(s) 360
NspV TTCGAA 1 cut(s) 366
PagI TCATGA 1 cut(s) 379
PfeI GAWTC 1 cut(s) 286
PkrI GCNGC 3 cut(s) 202, 589, 633
Ppu21I YACGTR 1 cut(s) 638
PsiI TTATAA 1 cut(s) 404
PspPI GGNCC 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 559
RsaI GTAC 2 cut(s) 469, 640
RsaNI GTAC 2 cut(s) 468, 639
RseI CAYNNNNRTG 1 cut(s) 490
SaqAI TTAA 2 cut(s) 345, 661
SatI GCNGC 3 cut(s) 201, 588, 632
Sau3AI GATC 5 cut(s) 15, 235, 533, 553, 592
Sau96I GGNCC 1 cut(s) 292
ScrFI CCNGG 1 cut(s) 340
SfaNI GCATC 2 cut(s) 31, 47
SfuI TTCGAA 1 cut(s) 366
SinI GGWCC 1 cut(s) 292
SmiMI CAYNNNNRTG 1 cut(s) 490
SnaBI TACGTA 1 cut(s) 638
Sse9I AATT 4 cut(s) 173, 346, 416, 626
SsiI CCGC 2 cut(s) 108, 632
SspMI CTAG 2 cut(s) 71, 102
StyD4I CCNGG 1 cut(s) 338
StyI CCWWGG 2 cut(s) 22, 460
TaiI ACGT 4 cut(s) 15, 469, 606, 640
TaqI TCGA 4 cut(s) 366, 536, 552, 595
TasI AATT 4 cut(s) 173, 346, 416, 626
TauI GCSGC 1 cut(s) 634
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 2 cut(s) 345, 661
Tru9I TTAA 2 cut(s) 345, 661
TseI GCWGC 2 cut(s) 200, 587
VpaK11BI GGWCC 1 cut(s) 292
XapI RAATTY 1 cut(s) 173
XceI RCATGY 1 cut(s) 360
XspI CTAG 2 cut(s) 71, 102
Zsp2I ATGCAT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.