Rorug03G0201900

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000265
Physical Location & Seq
Forward (+)
17767195 .. 17769057
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0201900.1

Sequence Viewer

Length: 1863 bp
ATGCTCAAATTGAAAAACCAGGCCTTCCTTATTCGAAACCATGCCATGCTTAGATTCCCCAAGTTTACTTCAGCAGCTGCCTTAGCTGATACCACAATTACCCAGACCCTTATACAGGAAGTGCCACAACCTCGAATTCAACCCCCCAAGCTGTTCATCACTGCCCTGCAGAGCTCTAGAAACATCTTCCACATTAGACAAGTCCATGCCCAAGTTGCTGTCAATGGAATCCTCCAAAACCTCACTGTTGCAAACAAGCTCCTCTACATGTATGCACAGCATAAGGTGTTGGGTGACGCTTATGCTCTGTTTAGTGGGATGGTGGAGAAGAATGCAGTTTCTTGGAGTGTAATGGTTGGTGGGTTTGTGAAGGGTGGTGACTTTGCTAATGGTTTTAGAACATTTAGGGAGCTCATTCGATGTGGGATGCCGCCGGATAATTACACATTGCCTTTTGTGATAAGGGCTTGTAGGGATTTGATGGACCTGAAAATTGGCAGGTTGGTTCATGACATTGTTCTGAAACATGGGATGCTTTCGGATAATTTTGTTTGTGCTGCTCTGGTGGACATGTATGCGAAATGTAGGGTTATACATGATGCCCGCCAACTGTTTGATAATATGCCTAACAGGGATATTGTAACATGGACAGTCATGATTGGTGGGTTTGCTGAGTGCAGGAATGCGAGTGAGTCTTTGGTTCTGTTTGATAGGATGATAGATCAAGGTGTTGTTCCAGATAAGGTTACTATGGTGACCGTTGTTAATGCTTGTGCAAAGTTTGGGGCTATGCATAAGGCCAGACTGGTTCATGATTATATACGTAGGAATAAGTTTTACTTGGATGTGATCTTGGGGACGGCGATGATTGATATGTATGCTAAGTGTGGGTGTGTTGGTTCCGCAAGGGAAATTTTCGACAGGATGCAAGTGAGGAATGTTAGAACATGGAGTGCCCTGATTGCGGCTTATGGTTATCATGGGCGCGGCAAGGAAGCTCTTGAGATATTTGAGATGATGTTGAGCAGTGGAGTGTTACCAAATGCAGTTACATTTATTTCACTATTATATGCTTGTAGTCATGCTGGGTTGACTGAAGAGGGTCTGCGGTTTTTCTCATTGATGTGGGATGAATATGCTGTCAGAGCAGATGTTAAACATTATACTTGTATGGTGGATCTTCTGGGTCGTGCTGGGAAACTTGATGAGGCCTTGAAATTGGCCGAGAGTATGGCAGTTGAGAAAGATGAGGGGTTATGGAGTGCTTTGCTTGGGGCCTGTAGAGTCCATGGGAATCTAGACCTAGCAGAAAAGGCAGCTAGTTCACTGCTTGAACTACAGCCCGAAAACCCAGGGCATTACGTATTGCTTTCAAATATTTATGCAAGAGCAGGGAGGTGGAAAGAAGTGGCGAAGATGAGAAATCTGATGACCCAGAGGAGACTAAAGAAAATTCCTGGTTATACTTGGATTGAGATAGACGACAAAAACTACCAATTTGGTGTTGCTGATAGAACTCATCCACAATCAGAAAACATTTATGCTATGCTGAAGAGTTTGACTCACAAACTGGAGTTGGCTGGTTATGTTCCTGATACGAATTTTGTGTTGCATGATGTTGATGAGGAAGTTAAAGTGGATATGTTGTACACCCATAGTGAGAAGTTGGCAATTGCATTTGGCCTTATCGCCACTTCTGATGGAACTCCTATTAGAATTACAAAGAATCTTAGGGTTTGTGGTGACTGCCACACATTTAGCAAGTTTGTATCAGCTATTACAAATAGGGAGATTATTGTCCGTGATGCAAACCGGTTTCACCTCTTCAGGGAAGGGACCTGCTCATGTGGAGATTATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

620

Amino Acids

69.93

Weight (kDa)

8.73

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 211 - 260 4.1e-09 PPR repeat family
PPR_2 PF13041 313 - 360 3.8e-10 PPR repeat family
PPR PF01535 316 - 345 5.5e-07 PPR repeat
E_motif PF20431 430 - 492 2.8e-24 E motif
DYW_deaminase PF14432 528 - 620 1.3e-44 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 489, 1847
AccII CGCG 1 cut(s) 987
AciI CCGC 6 cut(s) 431, 604, 903, 965, 987, 1108
AclWI GGATC 1 cut(s) 1185
AcoI YGGCCR 1 cut(s) 1221
AcsI RAATTY 4 cut(s) 135, 912, 1452, 1600
AcuI CTGAAG 4 cut(s) 54, 1116, 1571, 1810
AfaI GTAC 1 cut(s) 1649
AfiI CCNNNNNNNGG 3 cut(s) 115, 964, 1828
AflIII ACRYGT 2 cut(s) 267, 570
AgeI ACCGGT 1 cut(s) 1812
AgsI TTSAA 5 cut(s) 13, 140, 1216, 1334, 1374
AjnI CCWGG 3 cut(s) 18, 1351, 1456
AluBI AGCT 9 cut(s) 77, 86, 151, 174, 259, 412, 998, 1319, 1775
AluI AGCT 9 cut(s) 77, 86, 151, 174, 259, 412, 998, 1319, 1775
Alw21I GWGCWC 2 cut(s) 176, 414
Alw26I GTCTC 1 cut(s) 1435
AlwI GGATC 1 cut(s) 1185
AlwNI CAGNNNCTG 1 cut(s) 77
AoxI GGCC 6 cut(s) 21, 798, 1209, 1221, 1275, 1681
ApeKI GCWGC 4 cut(s) 74, 77, 557, 1316
ApoI RAATTY 4 cut(s) 135, 912, 1452, 1600
AsiGI ACCGGT 1 cut(s) 1812
AspLEI GCGC 1 cut(s) 987
AspS9I GGNCC 3 cut(s) 484, 1275, 1836
AsuHPI GGTGA 5 cut(s) 305, 389, 766, 1754, 1811
AsuII TTCGAA 1 cut(s) 34
AvaII GGWCC 2 cut(s) 484, 1836
BaeGI GKGCMC 1 cut(s) 958
BanII GRGCYC 2 cut(s) 176, 414
Bbv12I GWGCWC 2 cut(s) 176, 414
BbvI GCAGC 4 cut(s) 64, 86, 544, 1328
BccI CCATC 3 cut(s) 313, 475, 1694
BceAI ACGGC 1 cut(s) 876
BciT130I CCWGG 3 cut(s) 20, 1353, 1458
BcoDI GTCTC 1 cut(s) 1435
BfaI CTAG 4 cut(s) 177, 1298, 1304, 1320
BfmI CTRYAG 3 cut(s) 167, 1279, 1337
BfuAI ACCTGC 2 cut(s) 489, 1847
BisI GCNGC 7 cut(s) 75, 78, 431, 558, 966, 988, 1317
BlsI GCNGC 7 cut(s) 76, 79, 432, 559, 967, 989, 1318
Bme1390I CCNGG 3 cut(s) 20, 1353, 1458
Bme18I GGWCC 2 cut(s) 484, 1836
BmgT120I GGNCC 3 cut(s) 484, 1275, 1836
BmiI GGNNCC 3 cut(s) 901, 1276, 1837
BmrFI CCNGG 3 cut(s) 20, 1353, 1458
BmsI GCATC 5 cut(s) 417, 522, 589, 915, 1795
BplI GAGNNNNNCTC 2 cut(s) 1546, 1578
BpmI CTGGAG 1 cut(s) 1592
Bpu10I CCTNAGC 1 cut(s) 82
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 1022
BsaAI YACGTR 2 cut(s) 824, 1363
BsaJI CCNNGG 3 cut(s) 1288, 1351, 1352
BsaWI WCCGGW 1 cut(s) 1812
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 964, 1828
Bse118I RCCGGY 1 cut(s) 1812
Bse1I ACTGG 2 cut(s) 810, 1575
Bse3DI GCAATG 1 cut(s) 446
BseBI CCWGG 3 cut(s) 20, 1353, 1458
BseDI CCNNGG 3 cut(s) 1288, 1351, 1352
BseGI GGATG 8 cut(s) 324, 432, 537, 720, 850, 930, 1135, 1519
BseLI CCNNNNNNNGG 3 cut(s) 115, 964, 1828
BseMI GCAATG 1 cut(s) 446
BseMII CTCAG 1 cut(s) 663
BseNI ACTGG 2 cut(s) 810, 1575
BseRI GAGGAG 2 cut(s) 251, 1453
BseSI GKGCMC 1 cut(s) 958
BseXI GCAGC 4 cut(s) 64, 86, 544, 1328
BseYI CCCAGC 2 cut(s) 1085, 1193
BsgI GTGCAG 1 cut(s) 697
Bsh1236I CGCG 1 cut(s) 987
BshFI GGCC 6 cut(s) 23, 800, 1211, 1223, 1277, 1683
BshTI ACCGGT 1 cut(s) 1812
BsiHKAI GWGCWC 2 cut(s) 176, 414
BsiSI CCGG 2 cut(s) 434, 1813
BslFI GGGAC 2 cut(s) 871, 1849
BslI CCNNNNNNNGG 3 cut(s) 115, 964, 1828
BsmAI GTCTC 1 cut(s) 1435
BsmFI GGGAC 2 cut(s) 871, 1849
BsmI GAATGC 2 cut(s) 337, 688
BsnI GGCC 6 cut(s) 23, 800, 1211, 1223, 1277, 1683
Bsp119I TTCGAA 1 cut(s) 34
Bsp1286I GDGCHC 3 cut(s) 176, 414, 958
Bsp1407I TGTACA 1 cut(s) 1647
Bsp143I GATC 3 cut(s) 721, 849, 1177
Bsp19I CCATGG 1 cut(s) 1288
BspACI CCGC 6 cut(s) 431, 604, 903, 965, 987, 1108
BspANI GGCC 6 cut(s) 23, 800, 1211, 1223, 1277, 1683
BspCNI CTCAG 1 cut(s) 664
BspFNI CGCG 1 cut(s) 987
BspHI TCATGA 3 cut(s) 508, 654, 811
BspLI GGNNCC 3 cut(s) 901, 1276, 1837
BspMAI CTGCAG 1 cut(s) 171
BspMI ACCTGC 2 cut(s) 489, 1847
BspPI GGATC 1 cut(s) 1185
BspT104I TTCGAA 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 1812
BsrGI TGTACA 1 cut(s) 1647
BsrI ACTGG 2 cut(s) 810, 1575
BssAI RCCGGY 1 cut(s) 1812
BssECI CCNNGG 3 cut(s) 1288, 1351, 1352
BssMI GATC 3 cut(s) 721, 849, 1177
BssT1I CCWWGG 1 cut(s) 1288
Bst2UI CCWGG 3 cut(s) 20, 1353, 1458
Bst4CI ACNGT 4 cut(s) 247, 612, 652, 760
Bst6I CTCTTC 3 cut(s) 1092, 1547, 1829
BstAUI TGTACA 1 cut(s) 1647
BstBAI YACGTR 2 cut(s) 824, 1363
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 604
BstDEI CTNAG 5 cut(s) 50, 82, 672, 882, 1730
BstDSI CCRYGG 1 cut(s) 1288
BstEII GGTNACC 1 cut(s) 754
BstENI CCTNNNNNAGG 1 cut(s) 113
BstF5I GGATG 8 cut(s) 324, 432, 537, 720, 850, 930, 1135, 1519
BstFNI CGCG 1 cut(s) 987
BstHHI GCGC 1 cut(s) 987
BstKTI GATC 3 cut(s) 724, 852, 1180
BstMAI GTCTC 1 cut(s) 1435
BstMBI GATC 3 cut(s) 721, 849, 1177
BstMWI GCNNNNNNNGC 5 cut(s) 83, 215, 962, 1145, 1313
BstNI CCWGG 3 cut(s) 20, 1353, 1458
BstNSI RCATGY 2 cut(s) 271, 574
BstPI GGTNACC 1 cut(s) 754
BstSCI CCNGG 3 cut(s) 18, 1351, 1456
BstSFI CTRYAG 3 cut(s) 167, 1279, 1337
BstSLI GKGCMC 1 cut(s) 958
BstSNI TACGTA 2 cut(s) 824, 1363
BstUI CGCG 1 cut(s) 987
BstV1I GCAGC 4 cut(s) 64, 86, 544, 1328
BstX2I RGATCY 1 cut(s) 1177
BstYI RGATCY 1 cut(s) 1177
BsuRI GGCC 6 cut(s) 23, 800, 1211, 1223, 1277, 1683
BtgI CCRYGG 1 cut(s) 1288
BtgZI GCGATG 1 cut(s) 878
BtsCI GGATG 8 cut(s) 324, 432, 537, 720, 850, 930, 1135, 1519
BtsI GCAGTG 3 cut(s) 159, 1033, 1325
BtsIMutI CAGTG 4 cut(s) 159, 243, 1033, 1325
BveI ACCTGC 2 cut(s) 489, 1847
Cac8I GCNNGC 1 cut(s) 604
CaiI CAGNNNCTG 1 cut(s) 77
CciI TCATGA 3 cut(s) 508, 654, 811
CfoI GCGC 1 cut(s) 987
Cfr10I RCCGGY 1 cut(s) 1812
Cfr13I GGNCC 3 cut(s) 484, 1275, 1836
CseI GACGC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 1648
CspAI ACCGGT 1 cut(s) 1812
CviQI GTAC 1 cut(s) 1648
DdeI CTNAG 5 cut(s) 50, 82, 672, 882, 1730
DpnI GATC 3 cut(s) 723, 851, 1179
DpnII GATC 3 cut(s) 721, 849, 1177
EaeI YGGCCR 1 cut(s) 1221
Eam1104I CTCTTC 3 cut(s) 1092, 1547, 1829
EarI CTCTTC 3 cut(s) 1092, 1547, 1829
Ecl136II GAGCTC 2 cut(s) 174, 412
Eco105I TACGTA 2 cut(s) 824, 1363
Eco130I CCWWGG 1 cut(s) 1288
Eco147I AGGCCT 2 cut(s) 23, 1211
Eco24I GRGCYC 2 cut(s) 176, 414
Eco47I GGWCC 2 cut(s) 484, 1836
Eco53kI GAGCTC 2 cut(s) 174, 412
Eco57I CTGAAG 4 cut(s) 54, 1116, 1571, 1810
Eco91I GGTNACC 1 cut(s) 754
EcoICRI GAGCTC 2 cut(s) 174, 412
EcoNI CCTNNNNNAGG 1 cut(s) 113
EcoO109I RGGNCCY 2 cut(s) 1275, 1836
EcoO65I GGTNACC 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 135
EcoRII CCWGG 3 cut(s) 18, 1351, 1456
EcoT14I CCWWGG 1 cut(s) 1288
EcoT22I ATGCAT 1 cut(s) 795
EcoT38I GRGCYC 2 cut(s) 176, 414
ErhI CCWWGG 1 cut(s) 1288
FalI AAGNNNNNCTT 2 cut(s) 824, 856
FaqI GGGAC 2 cut(s) 871, 1849
FauI CCCGC 1 cut(s) 611
Fnu4HI GCNGC 7 cut(s) 75, 78, 431, 558, 966, 988, 1317
FokI GGATG 8 cut(s) 331, 439, 544, 727, 857, 937, 1142, 1506
FriOI GRGCYC 2 cut(s) 176, 414
Fsp4HI GCNGC 7 cut(s) 75, 78, 431, 558, 966, 988, 1317
FspBI CTAG 4 cut(s) 177, 1298, 1304, 1320
GlaI GCGC 1 cut(s) 986
GluI GCNGC 7 cut(s) 75, 78, 431, 558, 966, 988, 1317
GsaI CCCAGC 2 cut(s) 1089, 1197
GsuI CTGGAG 1 cut(s) 1592
HaeIII GGCC 6 cut(s) 23, 800, 1211, 1223, 1277, 1683
HapII CCGG 2 cut(s) 434, 1813
HgaI GACGC 1 cut(s) 305
HhaI GCGC 1 cut(s) 987
Hin6I GCGC 1 cut(s) 985
HinP1I GCGC 1 cut(s) 985
HincII GTYRAC 1 cut(s) 1092
HindII GTYRAC 1 cut(s) 1092
HinfI GANTC 7 cut(s) 54, 228, 692, 1284, 1294, 1561, 1726
HpaII CCGG 2 cut(s) 434, 1813
HphI GGTGA 5 cut(s) 305, 389, 766, 1754, 1811
Hpy166II GTNNAC 5 cut(s) 66, 568, 1092, 1325, 1650
Hpy188I TCNGA 6 cut(s) 522, 541, 1145, 1428, 1531, 1699
Hpy188III TCNNGA 8 cut(s) 177, 509, 655, 737, 812, 1001, 1298, 1592
Hpy8I GTNNAC 5 cut(s) 66, 568, 1092, 1325, 1650
HpyAV CCTTC 3 cut(s) 34, 364, 1826
HpyCH4III ACNGT 4 cut(s) 247, 612, 652, 760
HpyCH4IV ACGT 2 cut(s) 823, 1362
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 215, 962, 1145, 1313
HpyF3I CTNAG 5 cut(s) 50, 82, 672, 882, 1730
HpySE526I ACGT 2 cut(s) 823, 1362
HspAI GCGC 1 cut(s) 985
Kzo9I GATC 3 cut(s) 721, 849, 1177
LmnI GCTCC 2 cut(s) 264, 409
Lsp1109I GCAGC 4 cut(s) 64, 86, 544, 1328
LweI GCATC 5 cut(s) 417, 522, 589, 915, 1795
MaeI CTAG 4 cut(s) 177, 1298, 1304, 1320
MaeII ACGT 2 cut(s) 823, 1362
MaeIII GTNAC 8 cut(s) 293, 377, 640, 745, 754, 1035, 1048, 1742
MalI GATC 3 cut(s) 723, 851, 1179
MboI GATC 3 cut(s) 721, 849, 1177
MboII GAAGA 7 cut(s) 178, 340, 1109, 1172, 1426, 1564, 1816
MfeI CAATTG 1 cut(s) 1671
MflI RGATCY 1 cut(s) 1177
MhlI GDGCHC 3 cut(s) 176, 414, 958
MlyI GAGTC 3 cut(s) 701, 1293, 1555
Mph1103I ATGCAT 1 cut(s) 795
MseI TTAA 3 cut(s) 765, 1155, 1632
MslI CAYNNNNRTG 1 cut(s) 1123
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 2 cut(s) 434, 1813
MspR9I CCNGG 3 cut(s) 20, 1353, 1458
MunI CAATTG 1 cut(s) 1671
Mva1269I GAATGC 2 cut(s) 337, 688
MvaI CCWGG 3 cut(s) 20, 1353, 1458
MvnI CGCG 1 cut(s) 987
MwoI GCNNNNNNNGC 5 cut(s) 83, 215, 962, 1145, 1313
NcoI CCATGG 1 cut(s) 1288
NdeII GATC 3 cut(s) 721, 849, 1177
NlaIV GGNNCC 3 cut(s) 901, 1276, 1837
NmeAIII GCCGAG 1 cut(s) 1249
NmuCI GTSAC 4 cut(s) 293, 377, 754, 1742
NsiI ATGCAT 1 cut(s) 795
NspI RCATGY 2 cut(s) 271, 574
NspV TTCGAA 1 cut(s) 34
PagI TCATGA 3 cut(s) 508, 654, 811
PasI CCCWGGG 1 cut(s) 1352
PceI AGGCCT 2 cut(s) 23, 1211
PciI ACATGT 2 cut(s) 267, 570
PctI GAATGC 2 cut(s) 337, 688
PfeI GAWTC 4 cut(s) 54, 228, 1294, 1726
PinAI ACCGGT 1 cut(s) 1812
PkrI GCNGC 7 cut(s) 76, 79, 432, 559, 967, 989, 1318
PleI GAGTC 3 cut(s) 700, 1292, 1555
PpsI GAGTC 3 cut(s) 700, 1292, 1555
Ppu21I YACGTR 2 cut(s) 824, 1363
PpuMI RGGWCCY 1 cut(s) 1836
PscI ACATGT 2 cut(s) 267, 570
Psp124BI GAGCTC 2 cut(s) 176, 414
Psp5II RGGWCCY 1 cut(s) 1836
Psp6I CCWGG 3 cut(s) 18, 1351, 1456
PspEI GGTNACC 1 cut(s) 754
PspFI CCCAGC 2 cut(s) 1085, 1193
PspGI CCWGG 3 cut(s) 18, 1351, 1456
PspN4I GGNNCC 3 cut(s) 901, 1276, 1837
PspPI GGNCC 3 cut(s) 484, 1275, 1836
PspPPI RGGWCCY 1 cut(s) 1836
PstI CTGCAG 1 cut(s) 171
PstNI CAGNNNCTG 1 cut(s) 77
PsuI RGATCY 1 cut(s) 1177
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 1 cut(s) 1649
RsaNI GTAC 1 cut(s) 1648
RseI CAYNNNNRTG 1 cut(s) 1123
SacI GAGCTC 2 cut(s) 176, 414
SaqAI TTAA 3 cut(s) 765, 1155, 1632
SatI GCNGC 7 cut(s) 75, 78, 431, 558, 966, 988, 1317
Sau3AI GATC 3 cut(s) 721, 849, 1177
Sau96I GGNCC 3 cut(s) 484, 1275, 1836
SchI GAGTC 3 cut(s) 701, 1293, 1555
ScrFI CCNGG 3 cut(s) 20, 1353, 1458
SduI GDGCHC 3 cut(s) 176, 414, 958
SfaNI GCATC 5 cut(s) 417, 522, 589, 915, 1795
SfcI CTRYAG 3 cut(s) 167, 1279, 1337
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 2 cut(s) 484, 1836
SmiMI CAYNNNNRTG 1 cut(s) 1123
SmlI CTYRAG 1 cut(s) 1001
SmoI CTYRAG 1 cut(s) 1001
SnaBI TACGTA 2 cut(s) 824, 1363
SseBI AGGCCT 2 cut(s) 23, 1211
SsiI CCGC 6 cut(s) 431, 604, 903, 965, 987, 1108
SspI AATATT 1 cut(s) 1378
SspMI CTAG 4 cut(s) 177, 1298, 1304, 1320
SstI GAGCTC 2 cut(s) 176, 414
StuI AGGCCT 2 cut(s) 23, 1211
StyD4I CCNGG 3 cut(s) 18, 1351, 1456
StyI CCWWGG 1 cut(s) 1288
TaaI ACNGT 4 cut(s) 247, 612, 652, 760
TaiI ACGT 2 cut(s) 826, 1365
TaqI TCGA 4 cut(s) 34, 133, 418, 918
TatI WGTACW 1 cut(s) 1647
TauI GCSGC 3 cut(s) 433, 968, 990
TfiI GAWTC 4 cut(s) 54, 228, 1294, 1726
Tru1I TTAA 3 cut(s) 765, 1155, 1632
Tru9I TTAA 3 cut(s) 765, 1155, 1632
TscAI CASTG 4 cut(s) 166, 250, 1033, 1332
TseFI GTSAC 4 cut(s) 293, 377, 754, 1742
TseI GCWGC 4 cut(s) 74, 77, 557, 1316
Tsp45I GTSAC 4 cut(s) 293, 377, 754, 1742
TspDTI ATGAA 4 cut(s) 145, 497, 800, 1146
TspGWI ACGGA 1 cut(s) 1790
TspRI CASTG 4 cut(s) 166, 250, 1033, 1332
VpaK11BI GGWCC 2 cut(s) 484, 1836
XagI CCTNNNNNAGG 1 cut(s) 113
XapI RAATTY 4 cut(s) 135, 912, 1452, 1600
XbaI TCTAGA 2 cut(s) 176, 1297
XceI RCATGY 2 cut(s) 271, 574
XspI CTAG 4 cut(s) 177, 1298, 1304, 1320
Zsp2I ATGCAT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.