Rroxscaffold_7G00160950

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000265
Physical Location & Seq
Forward (+)
3805144 .. 3807524
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160950.1

Sequence Viewer

Length: 1062 bp
ATGGAGATGCCTATCCACGGATCCGCTTCTTTTCCAAATAATTTCATCAAGAGAGAAAGATCATTCCTTCAGTTTCCCACTTATTGGAACCTCGTCACTGTTCTTAGCATTGATGGAGGTGGAATCAGAGGAATTATCCCTGGTACTCTTCTCAGTTTCCTTGAGTCTGAGCTTCAGGATGCAAGACTGGCAGATTATTTTGATGTCATTGCAGGAACTAGTACTGGTGGCCTTGTGACTGCCATGCTTACCGCCCCGGATGAAAACGGCCGTCCATTATTTGCTGCTAAAGATATCAACAAATTTTACCTTGAACACTGCCCTAAGATATTTCCCCAAGATAGTTGCAAAATTGATTGTCTTCCATTAACTCCAATAACTGATGCGGTGGCGGCGGTAAAAGCTTTCAGAGGGCCAAAGTATGATGGGGAGTATTTACATAATATAGTGAAGGAAAAGTTAGGAGACATAAGATTGCATCAGACATTGACCAACGTCATAATTCCCACGTTTGATATAAAGAGGCTCCAACCAACCGTCTTCTCCAGCTATGAGGCTTTGGTGGCTATAAGTGAAGTGTCAAAAGAAATTTACAAGGGAAATCCCGACTTTTTTACAATGAAGGCAACTGAATACTCCCGGTTTCTAGTTGTATCATTGGGTACTGGTTCAGCAAAATGTGATGAGAAGTACGATGCCCAAAATACAGCTAAGTGGGGTATTTTGGGATGGTTGATCCGAGGCGGTTCCTGTCCGTTGGTTGATATATTTACTCAAGCAAGTAGTGATATGGTTGACTTCCACCTTTCTACAGTGTTCCAAGCCCTTCACTCTGAGAAAAATTACCTAAGAATCCAGGATGATGATTTGGGTAAGACATTGTGTTCTGTGGATATTGCTACAGAAGAAAACTTGCAAAATCTTGTAAAAGTTGGAGAAAATTTGTTAAAGAAACCAGTTTCCCGAGTAAACTTGGAAACCGGTATCCATGAGCCTGCCCATCATTCTACCAACAAAGAGGCTCTAGTAAGGTACGTATATATTAGATTAAGTAGTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

353

Amino Acids

39.36

Weight (kDa)

6.01

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 35 - 174 7.4e-13 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 84
AciI CCGC 6 cut(s) 24, 252, 386, 392, 395, 744
AclWI GGATC 3 cut(s) 15, 28, 730
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 3 cut(s) 302, 588, 940
AcuI CTGAAG 2 cut(s) 53, 158
AfaI GTAC 5 cut(s) 145, 223, 664, 692, 1034
AfiI CCNNNNNNNGG 2 cut(s) 17, 84
AgeI ACCGGT 1 cut(s) 980
AgsI TTSAA 1 cut(s) 314
AhlI ACTAGT 1 cut(s) 218
AjnI CCWGG 2 cut(s) 139, 855
AluBI AGCT 4 cut(s) 172, 404, 549, 710
AluI AGCT 4 cut(s) 172, 404, 549, 710
Alw26I GTCTC 1 cut(s) 459
AlwI GGATC 3 cut(s) 15, 28, 730
Ama87I CYCGRG 1 cut(s) 963
AoxI GGCC 3 cut(s) 229, 268, 413
ApeKI GCWGC 1 cut(s) 284
ApoI RAATTY 3 cut(s) 302, 588, 940
AsiGI ACCGGT 1 cut(s) 980
AspS9I GGNCC 1 cut(s) 413
AsuC2I CCSGG 2 cut(s) 257, 640
AvaI CYCGRG 1 cut(s) 963
BamHI GGATCC 1 cut(s) 20
BbsI GAAGAC 2 cut(s) 353, 532
BbvI GCAGC 1 cut(s) 271
BccI CCATC 4 cut(s) 107, 419, 723, 1008
BceAI ACGGC 2 cut(s) 255, 283
BciT130I CCWGG 2 cut(s) 141, 857
BciVI GTATCC 1 cut(s) 995
BcnI CCSGG 2 cut(s) 257, 640
BcoDI GTCTC 1 cut(s) 459
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 3 cut(s) 219, 647, 1025
BfmI CTRYAG 2 cut(s) 810, 900
BfuI GTATCC 1 cut(s) 995
BisI GCNGC 2 cut(s) 285, 393
BlsI GCNGC 2 cut(s) 286, 394
BmcAI AGTACT 1 cut(s) 223
Bme1390I CCNGG 4 cut(s) 141, 257, 640, 857
BmeT110I CYCGRG 1 cut(s) 963
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 4 cut(s) 22, 89, 527, 748
BmrFI CCNGG 4 cut(s) 141, 257, 640, 857
BmsI GCATC 4 cut(s) 169, 373, 487, 685
BoxI GACNNNNGTC 1 cut(s) 494
BpiI GAAGAC 2 cut(s) 353, 532
BpmI CTGGAG 1 cut(s) 529
BpuEI CTTGAG 2 cut(s) 182, 759
BpuMI CCSGG 2 cut(s) 257, 640
BsaAI YACGTR 1 cut(s) 1036
BsaBI GATNNNNATC 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 16, 139, 255, 739
BsaWI WCCGGW 1 cut(s) 980
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 84
Bse118I RCCGGY 1 cut(s) 980
Bse1I ACTGG 4 cut(s) 192, 229, 670, 956
Bse3DI GCAATG 1 cut(s) 207
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 2 cut(s) 141, 857
BseDI CCNNGG 4 cut(s) 16, 139, 255, 739
BseGI GGATG 4 cut(s) 184, 265, 734, 865
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 2 cut(s) 17, 84
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 3 cut(s) 159, 166, 825
BseNI ACTGG 4 cut(s) 192, 229, 670, 956
BseX3I CGGCCG 1 cut(s) 268
BseXI GCAGC 1 cut(s) 271
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 3 cut(s) 231, 270, 415
BshTI ACCGGT 1 cut(s) 980
BsiEI CGRYCG 1 cut(s) 271
BsiHKCI CYCGRG 1 cut(s) 963
BsiSI CCGG 3 cut(s) 257, 640, 981
BslI CCNNNNNNNGG 2 cut(s) 17, 84
BsmAI GTCTC 1 cut(s) 459
BsnI GGCC 3 cut(s) 231, 270, 415
BsoBI CYCGRG 1 cut(s) 963
Bsp143I GATC 3 cut(s) 20, 59, 735
BspACI CCGC 6 cut(s) 24, 252, 386, 392, 395, 744
BspANI GGCC 3 cut(s) 231, 270, 415
BspCNI CTCAG 3 cut(s) 160, 165, 826
BspLI GGNNCC 4 cut(s) 22, 89, 527, 748
BspPI GGATC 3 cut(s) 15, 28, 730
BsrDI GCAATG 1 cut(s) 207
BsrFI RCCGGY 1 cut(s) 980
BsrI ACTGG 4 cut(s) 192, 229, 670, 956
BssAI RCCGGY 1 cut(s) 980
BssECI CCNNGG 4 cut(s) 16, 139, 255, 739
BssMI GATC 3 cut(s) 20, 59, 735
Bst2UI CCWGG 2 cut(s) 141, 857
Bst4CI ACNGT 3 cut(s) 100, 538, 814
Bst6I CTCTTC 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 1036
BstC8I GCNNGC 1 cut(s) 996
BstDEI CTNAG 7 cut(s) 104, 152, 168, 324, 711, 834, 848
BstDSI CCRYGG 1 cut(s) 16
BstF5I GGATG 4 cut(s) 184, 265, 734, 865
BstKTI GATC 3 cut(s) 23, 62, 738
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 3 cut(s) 20, 59, 735
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 4 cut(s) 188, 392, 401, 563
BstNI CCWGG 2 cut(s) 141, 857
BstPAI GACNNNNGTC 1 cut(s) 494
BstSCI CCNGG 4 cut(s) 139, 255, 638, 855
BstSFI CTRYAG 2 cut(s) 810, 900
BstSNI TACGTA 1 cut(s) 1036
BstV1I GCAGC 1 cut(s) 271
BstV2I GAAGAC 2 cut(s) 353, 532
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
BstZI CGGCCG 1 cut(s) 268
BsuI GTATCC 1 cut(s) 995
BsuRI GGCC 3 cut(s) 231, 270, 415
BtgI CCRYGG 1 cut(s) 16
BtsCI GGATG 4 cut(s) 184, 265, 734, 865
BtsI GCAGTG 1 cut(s) 316
BtsIMutI CAGTG 3 cut(s) 96, 316, 819
Cac8I GCNNGC 1 cut(s) 996
Cfr10I RCCGGY 1 cut(s) 980
Cfr13I GGNCC 1 cut(s) 413
Csp6I GTAC 5 cut(s) 144, 222, 663, 691, 1033
CspAI ACCGGT 1 cut(s) 980
CviAII CATG 2 cut(s) 244, 989
CviQI GTAC 5 cut(s) 144, 222, 663, 691, 1033
DdeI CTNAG 7 cut(s) 104, 152, 168, 324, 711, 834, 848
DpnI GATC 3 cut(s) 22, 61, 737
DpnII GATC 3 cut(s) 20, 59, 735
EaeI YGGCCR 1 cut(s) 268
EagI CGGCCG 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
EclXI CGGCCG 1 cut(s) 268
Eco105I TACGTA 1 cut(s) 1036
Eco32I GATATC 1 cut(s) 295
Eco52I CGGCCG 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 53, 158
Eco88I CYCGRG 1 cut(s) 963
EcoRII CCWGG 2 cut(s) 139, 855
EcoRV GATATC 1 cut(s) 295
FaeI CATG 2 cut(s) 247, 992
FatI CATG 2 cut(s) 243, 988
Fnu4HI GCNGC 2 cut(s) 285, 393
FokI GGATG 4 cut(s) 191, 272, 741, 872
Fsp4HI GCNGC 2 cut(s) 285, 393
FspBI CTAG 3 cut(s) 219, 647, 1025
GluI GCNGC 2 cut(s) 285, 393
GsuI CTGGAG 1 cut(s) 529
HaeIII GGCC 3 cut(s) 231, 270, 415
HapII CCGG 3 cut(s) 257, 640, 981
Hin1II CATG 2 cut(s) 247, 992
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 1 cut(s) 402
HinfI GANTC 3 cut(s) 123, 164, 852
HpaII CCGG 3 cut(s) 257, 640, 981
Hpy166II GTNNAC 2 cut(s) 796, 970
Hpy188I TCNGA 6 cut(s) 128, 169, 410, 483, 740, 835
Hpy188III TCNNGA 4 cut(s) 49, 176, 605, 963
Hpy8I GTNNAC 2 cut(s) 796, 970
HpyAV CCTTC 4 cut(s) 77, 445, 616, 836
HpyCH4III ACNGT 3 cut(s) 100, 538, 814
HpyCH4IV ACGT 3 cut(s) 495, 509, 1035
HpyCH4V TGCA 5 cut(s) 182, 212, 348, 478, 916
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 392, 401, 563
HpyF3I CTNAG 7 cut(s) 104, 152, 168, 324, 711, 834, 848
HpySE526I ACGT 3 cut(s) 495, 509, 1035
Hsp92II CATG 2 cut(s) 247, 992
Kzo9I GATC 3 cut(s) 20, 59, 735
LmnI GCTCC 1 cut(s) 531
Lsp1109I GCAGC 1 cut(s) 271
LweI GCATC 4 cut(s) 169, 373, 487, 685
MaeI CTAG 3 cut(s) 219, 647, 1025
MaeII ACGT 3 cut(s) 495, 509, 1035
MaeIII GTNAC 2 cut(s) 94, 235
MalI GATC 3 cut(s) 22, 61, 737
MboI GATC 3 cut(s) 20, 59, 735
MboII GAAGA 4 cut(s) 140, 353, 532, 917
MflI RGATCY 1 cut(s) 20
MluCI AATT 8 cut(s) 40, 132, 302, 351, 501, 588, 841, 940
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 2 cut(s) 553, 913
MnlI CCTC 8 cut(s) 101, 110, 122, 404, 516, 547, 734, 1012
MseI TTAA 3 cut(s) 368, 947, 1049
MspI CCGG 3 cut(s) 257, 640, 981
MspR9I CCNGG 4 cut(s) 141, 257, 640, 857
MvaI CCWGG 2 cut(s) 141, 857
MwoI GCNNNNNNNGC 4 cut(s) 188, 392, 401, 563
NciI CCSGG 2 cut(s) 257, 640
NdeII GATC 3 cut(s) 20, 59, 735
NlaIII CATG 2 cut(s) 247, 992
NlaIV GGNNCC 4 cut(s) 22, 89, 527, 748
NmuCI GTSAC 2 cut(s) 94, 235
PfeI GAWTC 2 cut(s) 123, 852
PflMI CCANNNNNTGG 1 cut(s) 84
PfoI TCCNGGA 1 cut(s) 855
PinAI ACCGGT 1 cut(s) 980
PkrI GCNGC 2 cut(s) 286, 394
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
Ppu21I YACGTR 1 cut(s) 1036
PshAI GACNNNNGTC 1 cut(s) 494
Psp6I CCWGG 2 cut(s) 139, 855
PspGI CCWGG 2 cut(s) 139, 855
PspN4I GGNNCC 4 cut(s) 22, 89, 527, 748
PspPI GGNCC 1 cut(s) 413
PsuI RGATCY 1 cut(s) 20
RsaI GTAC 5 cut(s) 145, 223, 664, 692, 1034
RsaNI GTAC 5 cut(s) 144, 222, 663, 691, 1033
SaqAI TTAA 3 cut(s) 368, 947, 1049
SatI GCNGC 2 cut(s) 285, 393
Sau3AI GATC 3 cut(s) 20, 59, 735
Sau96I GGNCC 1 cut(s) 413
ScaI AGTACT 1 cut(s) 223
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 4 cut(s) 141, 257, 640, 857
SfaNI GCATC 4 cut(s) 169, 373, 487, 685
SfcI CTRYAG 2 cut(s) 810, 900
SmlI CTYRAG 2 cut(s) 161, 774
SmoI CTYRAG 2 cut(s) 161, 774
SnaBI TACGTA 1 cut(s) 1036
SpeI ACTAGT 1 cut(s) 218
Sse9I AATT 8 cut(s) 40, 132, 302, 351, 501, 588, 841, 940
SsiI CCGC 6 cut(s) 24, 252, 386, 392, 395, 744
SspMI CTAG 3 cut(s) 219, 647, 1025
StyD4I CCNGG 4 cut(s) 139, 255, 638, 855
TaaI ACNGT 3 cut(s) 100, 538, 814
TaiI ACGT 3 cut(s) 498, 512, 1038
TasI AATT 8 cut(s) 40, 132, 302, 351, 501, 588, 841, 940
TatI WGTACW 1 cut(s) 221
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 2 cut(s) 123, 852
Tru1I TTAA 3 cut(s) 368, 947, 1049
Tru9I TTAA 3 cut(s) 368, 947, 1049
TscAI CASTG 3 cut(s) 103, 323, 819
TseFI GTSAC 2 cut(s) 94, 235
TseI GCWGC 1 cut(s) 284
Tsp45I GTSAC 2 cut(s) 94, 235
TspDTI ATGAA 3 cut(s) 34, 276, 635
TspGWI ACGGA 2 cut(s) 33, 744
TspRI CASTG 3 cut(s) 103, 323, 819
Van91I CCANNNNNTGG 1 cut(s) 84
XapI RAATTY 3 cut(s) 302, 588, 940
XspI CTAG 3 cut(s) 219, 647, 1025
ZrmI AGTACT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.