Rh6BG431800

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000265
Physical Location & Seq
Reverse (-)
65149004 .. 65161080
12077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG431800.1

Sequence Viewer

Length: 1260 bp
ATGGAAAGAACCAAGAGTATACCTCTACAGCCCCCAACCTACGGAAACCTAGTAACTGTTTTGAGCATTGATGGGGGTGGAATAAGAGGCCTTATCCCAGCAGCCATTCTTGATTACCTTGAATCCGAGCTTCAGAAGCTAGATGGCAAGGATGCAAGACTAGCAGATTATTTTGATGTGATTGCCGGGACTAGCACAGGTGGTCTCGTAACTGCCATGATTGCAACCCCAGACGAGAACAACAACAACCGCCCTGTGTTTGCCGCAAAAGATATTAAGGATTTCTATCTTACACACTGCCCTAAAATCTTCCCCCAGAGGCACTGTCCAGTGTTTCCTCTTACTGTAAAGATTATCAAAGCTTTAGCAGGACCAAAATATGATGGAAAATATCTCCACCACTTGGTTGGGGAAAAATTAGGTGACAAAAAACTGCACAAGACATTGACTAATGTGGTCATTCCCACTTTTGACATAAAACAACTCCAGCCAACTATCTTTTCCAGCTTTGAGGTGAAAAGCAAGCCATGCTCCGATGCTCTACTCAGAGATATATGCATTGCAACCTCAGCTGCACCTACTTATCTCCCAGCTCATTATTTTGAAACCCAAGACTTCCAAGGCAACGTTAAGGAATTTAACCTTATTGATGGCGGCGTTTGTGCAAATAACCCGACTTTAGTTGCTATTGGTGAAGTGACAAAGACAATACTCAAGGGCAGTTCAAACTTCTTTCCTATTAAACCCATGGACTATGGTCGATTTCTAGTCATATCACTAGGAACTGGCTCACCCAAGATAGAACTGAAATATCATGCCCATGCTGCTGCCAAATGGGGTTTGTTGGATTGGTTAACCAGCGGTGGACATACCCCATTAATTGATGTTTTCAGTCAGTCAAGTTCTGATATGGTCGATTTTAATCTGTCGGTTGTTTTCCAAGCCCTACACTCGGAGGAAAACTATCTTCGAATTCAGGATGACACGCTAACTGGGCAAGTATCTTCAGTGGATATAGCAGTAGAGAAGAATTTGAATGATCTTTTGAAAGTTGGTGAAGGCCTATTGAAAAAACCAGTCTCTAGAATTAATTTAGAGACCGGAAATTATGAGGCTTTTACCGAAGAGACTAATGCAGAGGCTCTTACAAGGTTTGCAAAACTACTGTCTGAAGAGAAGTGGCTTCGCCTTGCTAGGTCCCCCCAGGGACATGCTATACCTAAATCTAGTCATGATGGCTCTCTTAGTATTAAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

419

Amino Acids

46.0

Weight (kDa)

6.47

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 21 - 230 7.1e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 403
AccI GTMKAC 1 cut(s) 19
AciI CCGC 4 cut(s) 250, 264, 654, 861
AclI AACGTT 1 cut(s) 627
AcsI RAATTY 3 cut(s) 635, 972, 1030
AcuI CTGAAG 3 cut(s) 116, 990, 1191
AfiI CCNNNNNNNGG 3 cut(s) 41, 403, 952
AgsI TTSAA 6 cut(s) 122, 605, 726, 1036, 1048, 1069
AjnI CCWGG 1 cut(s) 1203
AjuI GAANNNNNNNTTGG 2 cut(s) 484, 516
AluBI AGCT 6 cut(s) 130, 139, 362, 507, 572, 593
AluI AGCT 6 cut(s) 130, 139, 362, 507, 572, 593
Alw26I GTCTC 4 cut(s) 209, 1084, 1091, 1121
AoxI GGCC 2 cut(s) 88, 1060
ApeKI GCWGC 4 cut(s) 101, 572, 824, 827
ApoI RAATTY 3 cut(s) 635, 972, 1030
ArsI GACNNNNNNTTYG 2 cut(s) 1151, 1183
AseI ATTAAT 2 cut(s) 878, 1089
AspS9I GGNCC 2 cut(s) 371, 1197
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 5 cut(s) 434, 526, 704, 783, 1067
AsuII TTCGAA 1 cut(s) 970
AvaII GGWCC 2 cut(s) 371, 1197
BbvCI CCTCAGC 1 cut(s) 568
BbvI GCAGC 4 cut(s) 113, 559, 811, 814
BccI CCATC 5 cut(s) 65, 137, 377, 644, 1229
BciT130I CCWGG 1 cut(s) 1205
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 4 cut(s) 209, 1084, 1091, 1121
BfaI CTAG 9 cut(s) 50, 140, 161, 192, 767, 779, 1083, 1194, 1227
BfmI CTRYAG 1 cut(s) 26
BisI GCNGC 6 cut(s) 102, 264, 573, 655, 825, 828
BlsI GCNGC 6 cut(s) 103, 265, 574, 656, 826, 829
Bme1390I CCNGG 2 cut(s) 187, 1205
Bme18I GGWCC 2 cut(s) 371, 1197
BmgT120I GGNCC 2 cut(s) 371, 1197
BmiI GGNNCC 1 cut(s) 1199
BmrFI CCNGG 2 cut(s) 187, 1205
BmrI ACTGGG 1 cut(s) 1002
BmsI GCATC 2 cut(s) 142, 526
BmuI ACTGGG 1 cut(s) 1002
BoxI GACNNNNGTC 1 cut(s) 756
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 470
Bpu10I CCTNAGC 1 cut(s) 568
Bpu14I TTCGAA 1 cut(s) 970
BpuEI CTTGAG 1 cut(s) 698
BpuMI CCSGG 1 cut(s) 187
BsaBI GATNNNNATC 2 cut(s) 285, 921
BsaI GGTCTC 2 cut(s) 209, 1091
BsaJI CCNNGG 4 cut(s) 619, 747, 1203, 1204
BsaWI WCCGGW 1 cut(s) 1100
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 403, 952
Bse1I ACTGG 4 cut(s) 329, 790, 997, 1076
Bse3DI GCAATG 1 cut(s) 558
Bse8I GATNNNNATC 2 cut(s) 285, 921
BseBI CCWGG 1 cut(s) 1205
BseDI CCNNGG 4 cut(s) 619, 747, 1203, 1204
BseGI GGATG 2 cut(s) 157, 985
BseJI GATNNNNATC 2 cut(s) 285, 921
BseLI CCNNNNNNNGG 3 cut(s) 41, 403, 952
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 2 cut(s) 559, 582
BseNI ACTGG 4 cut(s) 329, 790, 997, 1076
BseXI GCAGC 4 cut(s) 113, 559, 811, 814
BseYI CCCAGC 2 cut(s) 97, 589
BsgI GTGCAG 2 cut(s) 419, 558
BshFI GGCC 2 cut(s) 90, 1062
BsiSI CCGG 2 cut(s) 186, 1101
BslFI GGGAC 3 cut(s) 202, 1183, 1221
BslI CCNNNNNNNGG 3 cut(s) 41, 403, 952
BsmAI GTCTC 4 cut(s) 209, 1084, 1091, 1121
BsmFI GGGAC 3 cut(s) 202, 1183, 1221
BsnI GGCC 2 cut(s) 90, 1062
Bso31I GGTCTC 2 cut(s) 209, 1091
Bsp119I TTCGAA 1 cut(s) 970
Bsp143I GATC 1 cut(s) 1039
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 4 cut(s) 250, 264, 654, 861
BspANI GGCC 2 cut(s) 90, 1062
BspCNI CTCAG 2 cut(s) 558, 581
BspHI TCATGA 1 cut(s) 1231
BspLI GGNNCC 1 cut(s) 1199
BspT104I TTCGAA 1 cut(s) 970
BspTNI GGTCTC 2 cut(s) 209, 1091
BsrDI GCAATG 1 cut(s) 558
BsrI ACTGG 4 cut(s) 329, 790, 997, 1076
BssECI CCNNGG 4 cut(s) 619, 747, 1203, 1204
BssMI GATC 1 cut(s) 1039
BssNAI GTATAC 1 cut(s) 20
BssT1I CCWWGG 2 cut(s) 619, 747
Bst1107I GTATAC 1 cut(s) 20
Bst2UI CCWGG 1 cut(s) 1205
Bst4CI ACNGT 4 cut(s) 58, 326, 346, 1167
Bst6I CTCTTC 2 cut(s) 1119, 1167
BstAPI GCANNNNNTGC 1 cut(s) 528
BstBI TTCGAA 1 cut(s) 970
BstC8I GCNNGC 1 cut(s) 524
BstDEI CTNAG 3 cut(s) 545, 568, 1244
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 2 cut(s) 157, 985
BstKTI GATC 1 cut(s) 1042
BstMAI GTCTC 4 cut(s) 209, 1084, 1091, 1121
BstMBI GATC 1 cut(s) 1039
BstMWI GCNNNNNNNGC 7 cut(s) 136, 161, 221, 528, 569, 824, 994
BstNI CCWGG 1 cut(s) 1205
BstNSI RCATGY 1 cut(s) 1214
BstPAI GACNNNNGTC 1 cut(s) 756
BstSCI CCNGG 2 cut(s) 185, 1203
BstSFI CTRYAG 1 cut(s) 26
BstV1I GCAGC 4 cut(s) 113, 559, 811, 814
BstXI CCANNNNNNTGG 1 cut(s) 407
BstZ17I GTATAC 1 cut(s) 20
BsuRI GGCC 2 cut(s) 90, 1062
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 2 cut(s) 157, 985
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 4 cut(s) 295, 322, 336, 1014
Cac8I GCNNGC 1 cut(s) 524
CciI TCATGA 1 cut(s) 1231
Cfr13I GGNCC 2 cut(s) 371, 1197
CviAII CATG 7 cut(s) 217, 528, 748, 815, 821, 1211, 1232
DdeI CTNAG 3 cut(s) 545, 568, 1244
DpnI GATC 1 cut(s) 1041
DpnII GATC 1 cut(s) 1039
Eam1104I CTCTTC 2 cut(s) 1119, 1167
EarI CTCTTC 2 cut(s) 1119, 1167
Eco130I CCWWGG 2 cut(s) 619, 747
Eco147I AGGCCT 2 cut(s) 90, 1062
Eco31I GGTCTC 2 cut(s) 209, 1091
Eco47I GGWCC 2 cut(s) 371, 1197
Eco57I CTGAAG 3 cut(s) 116, 990, 1191
EcoO109I RGGNCCY 1 cut(s) 1197
EcoRI GAATTC 1 cut(s) 972
EcoRII CCWGG 1 cut(s) 1203
EcoT14I CCWWGG 2 cut(s) 619, 747
EcoT22I ATGCAT 1 cut(s) 560
ErhI CCWWGG 2 cut(s) 619, 747
FaeI CATG 7 cut(s) 220, 531, 751, 818, 824, 1214, 1235
FaqI GGGAC 3 cut(s) 202, 1183, 1221
FatI CATG 7 cut(s) 216, 527, 747, 814, 820, 1210, 1231
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 6 cut(s) 102, 264, 573, 655, 825, 828
FokI GGATG 2 cut(s) 164, 992
Fsp4HI GCNGC 6 cut(s) 102, 264, 573, 655, 825, 828
FspBI CTAG 9 cut(s) 50, 140, 161, 192, 767, 779, 1083, 1194, 1227
GluI GCNGC 6 cut(s) 102, 264, 573, 655, 825, 828
GsaI CCCAGC 2 cut(s) 101, 593
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 2 cut(s) 90, 1062
HapII CCGG 2 cut(s) 186, 1101
Hin1II CATG 7 cut(s) 220, 531, 751, 818, 824, 1214, 1235
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HindIII AAGCTT 1 cut(s) 360
HinfI GANTC 1 cut(s) 122
HpaI GTTAAC 1 cut(s) 855
HpaII CCGG 2 cut(s) 186, 1101
HphI GGTGA 5 cut(s) 434, 526, 704, 783, 1067
Hpy166II GTNNAC 3 cut(s) 20, 855, 866
Hpy188I TCNGA 7 cut(s) 127, 135, 535, 548, 907, 955, 1171
Hpy188III TCNNGA 4 cut(s) 110, 977, 1083, 1232
Hpy8I GTNNAC 3 cut(s) 20, 855, 866
HpyAV CCTTC 1 cut(s) 1052
HpyCH4III ACNGT 4 cut(s) 58, 326, 346, 1167
HpyCH4IV ACGT 1 cut(s) 627
HpyCH4V TGCA 9 cut(s) 155, 224, 436, 558, 563, 575, 665, 1136, 1157
HpyF10VI GCNNNNNNNGC 7 cut(s) 136, 161, 221, 528, 569, 824, 994
HpyF3I CTNAG 3 cut(s) 545, 568, 1244
HpySE526I ACGT 1 cut(s) 627
Hsp92II CATG 7 cut(s) 220, 531, 751, 818, 824, 1214, 1235
KspAI GTTAAC 1 cut(s) 855
Kzo9I GATC 1 cut(s) 1039
LmnI GCTCC 1 cut(s) 536
Lsp1109I GCAGC 4 cut(s) 113, 559, 811, 814
LweI GCATC 2 cut(s) 142, 526
MaeI CTAG 9 cut(s) 50, 140, 161, 192, 767, 779, 1083, 1194, 1227
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 4 cut(s) 52, 208, 422, 697
MalI GATC 1 cut(s) 1041
MboI GATC 1 cut(s) 1039
MboII GAAGA 6 cut(s) 301, 959, 996, 1039, 1136, 1184
MluCI AATT 8 cut(s) 416, 635, 879, 972, 1030, 1086, 1090, 1105
MmeI TCCRAC 1 cut(s) 825
MnlI CCTC 9 cut(s) 33, 80, 312, 348, 505, 577, 949, 1105, 1132
Mph1103I ATGCAT 1 cut(s) 560
MseI TTAA 9 cut(s) 276, 630, 639, 741, 854, 878, 921, 1089, 1251
MslI CAYNNNNRTG 1 cut(s) 819
MspA1I CMGCKG 2 cut(s) 572, 861
MspI CCGG 2 cut(s) 186, 1101
MspR9I CCNGG 2 cut(s) 187, 1205
MvaI CCWGG 1 cut(s) 1205
MwoI GCNNNNNNNGC 7 cut(s) 136, 161, 221, 528, 569, 824, 994
NciI CCSGG 1 cut(s) 187
NcoI CCATGG 1 cut(s) 747
NdeII GATC 1 cut(s) 1039
NlaIII CATG 7 cut(s) 220, 531, 751, 818, 824, 1214, 1235
NlaIV GGNNCC 1 cut(s) 1199
NmuCI GTSAC 2 cut(s) 422, 697
NsiI ATGCAT 1 cut(s) 560
NspI RCATGY 1 cut(s) 1214
NspV TTCGAA 1 cut(s) 970
PagI TCATGA 1 cut(s) 1231
PasI CCCWGGG 1 cut(s) 1204
PceI AGGCCT 2 cut(s) 90, 1062
PfeI GAWTC 1 cut(s) 122
PflMI CCANNNNNTGG 1 cut(s) 403
PkrI GCNGC 6 cut(s) 103, 265, 574, 656, 826, 829
PpuMI RGGWCCY 1 cut(s) 1197
PshAI GACNNNNGTC 1 cut(s) 756
PshBI ATTAAT 2 cut(s) 878, 1089
Psp1406I AACGTT 1 cut(s) 627
Psp5II RGGWCCY 1 cut(s) 1197
Psp6I CCWGG 1 cut(s) 1203
PspFI CCCAGC 2 cut(s) 97, 589
PspGI CCWGG 1 cut(s) 1203
PspN4I GGNNCC 1 cut(s) 1199
PspPI GGNCC 2 cut(s) 371, 1197
PspPPI RGGWCCY 1 cut(s) 1197
PvuII CAGCTG 1 cut(s) 572
RseI CAYNNNNRTG 1 cut(s) 819
SaqAI TTAA 9 cut(s) 276, 630, 639, 741, 854, 878, 921, 1089, 1251
SatI GCNGC 6 cut(s) 102, 264, 573, 655, 825, 828
Sau3AI GATC 1 cut(s) 1039
Sau96I GGNCC 2 cut(s) 371, 1197
ScrFI CCNGG 2 cut(s) 187, 1205
SfaNI GCATC 2 cut(s) 142, 526
SfcI CTRYAG 1 cut(s) 26
SfuI TTCGAA 1 cut(s) 970
SinI GGWCC 2 cut(s) 371, 1197
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse9I AATT 8 cut(s) 416, 635, 879, 972, 1030, 1086, 1090, 1105
SseBI AGGCCT 2 cut(s) 90, 1062
SsiI CCGC 4 cut(s) 250, 264, 654, 861
SspMI CTAG 9 cut(s) 50, 140, 161, 192, 767, 779, 1083, 1194, 1227
StuI AGGCCT 2 cut(s) 90, 1062
StyD4I CCNGG 2 cut(s) 185, 1203
StyI CCWWGG 2 cut(s) 619, 747
TaaI ACNGT 4 cut(s) 58, 326, 346, 1167
TaiI ACGT 1 cut(s) 630
TaqI TCGA 3 cut(s) 760, 915, 970
TasI AATT 8 cut(s) 416, 635, 879, 972, 1030, 1086, 1090, 1105
TauI GCSGC 2 cut(s) 266, 657
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 9 cut(s) 276, 630, 639, 741, 854, 878, 921, 1089, 1251
Tru9I TTAA 9 cut(s) 276, 630, 639, 741, 854, 878, 921, 1089, 1251
TscAI CASTG 4 cut(s) 302, 329, 336, 1014
TseFI GTSAC 2 cut(s) 422, 697
TseI GCWGC 4 cut(s) 101, 572, 824, 827
Tsp45I GTSAC 2 cut(s) 422, 697
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 4 cut(s) 302, 329, 336, 1014
Van91I CCANNNNNTGG 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 371, 1197
VspI ATTAAT 2 cut(s) 878, 1089
XapI RAATTY 3 cut(s) 635, 972, 1030
XbaI TCTAGA 1 cut(s) 1082
XceI RCATGY 1 cut(s) 1214
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 9 cut(s) 50, 140, 161, 192, 767, 779, 1083, 1194, 1227
Zsp2I ATGCAT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.