MD08G1105300.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000265
Physical Location & Seq
Reverse (-)
8958389 .. 8960583
2195 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1105300.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGGAAAGAACCACTAGCATTCCCCTTCAGCCCCCAACTTATGGAAACCTAATCACTGTTCTAAGCATTGATGGGGGTGGAATAAGAGGACTTATCCCAGGAACCATCCTTGCTTTCCTAGAATCTGAGCTTCAGAAGCTGGATGGTGAGGATGCAAGACTTGCCGACTATTTTGATGTGATTTCAGGAACAAGTACAGGTGGTCTTGTGACTGCCATGCTAACAACTCCAGATGAGAATAACCGTCCTGTATTTGCTGCCAAAGATATAAAGGATTTCTACCTTACTGAGATCCCTAAAATCTTCCCCCAGAATACTTGTTGGTTGTTTCCTTATATTACGAAGATTATCAAAAGTCTAACAGGACCAAAGTACAATGGGAAGTATCTGCATAGCTTGGTTAGGCAAAAGCTTGGTGAAAAAAAGCTCCACCAAACATTGACTAATGTTGTCATTCCTACATTTGACATAAAGAATCTCCAGCCAACAATCTTTTCTAGCTTTGAGGTGAAACATAAGCCATCTTTTGATGCCTTAGTCTCAGACATATGCATTGCAACCTCAGCAGCACCAACTTATCTTCCAGCTCATTATTTTGAAACCAAGGACCACAACGGAAATGTTAGAGAATTCAACCTAATCGATGGTGGAGTGGCTGCAAATAATCCGACTTTAGTTGCTATTGGTGAAGTGACAAAGCAAATAATCAGGGGTAGCCCGGACTTCTTTCCTATCAAACCTATGGATTATGGACGATATCTAGTCATATCATTAGGAACTGGCTCACCAAAAGCTGAATTGAAATATGATGCACATGATGCTGCTAAATGGGGCTTATTGAATTGGTTAACCAGCGGAGGCTCGACACCGATCATCAATGTTTTCAGTCACGCAAGTGCTGATATGGTTGATCTGAACCTTTCTGTGGTTTTCCAAGCCCTTCACTCGGAGAAAAATTACCTTCGAATTCAGGATGACAAGCTGCATGGGGACGTATCTTCTATGGATATAGCAACAGAGAAGAATTTGGACAATCTTGTGAAAGTTGGTGAAGGCCTATTGAAACAACCTGTTTCTAGGGTTAATTTAGAGACAGGAAAATTCGAAGCTTGTAATCATGAGACTAATGAAGAAGCTCTTATAAGGTTTGCAAAACTACTCTCTGAAGAGAAGTGGCTTCGTCTTGCAAAGTCGCCACAGGGTCAATGTACTGCAAAACCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

409

Amino Acids

45.03

Weight (kDa)

6.22

Isoelectric Point (pI)

29.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 21 - 228 6.4e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1142
AccB7I CCANNNNNTGG 1 cut(s) 41
AciI CCGC 1 cut(s) 855
AclWI GGATC 1 cut(s) 286
AcsI RAATTY 4 cut(s) 629, 966, 1024, 1100
AcuI CTGAAG 3 cut(s) 11, 116, 1185
AfaI GTAC 3 cut(s) 196, 374, 1210
AfiI CCNNNNNNNGG 3 cut(s) 41, 925, 946
AgsI TTSAA 5 cut(s) 599, 634, 802, 841, 1063
AjnI CCWGG 1 cut(s) 97
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AleI CACNNNNGTG 1 cut(s) 894
Alw26I GTCTC 3 cut(s) 544, 1085, 1115
AlwI GGATC 1 cut(s) 286
AlwNI CAGNNNCTG 1 cut(s) 139
AoxI GGCC 1 cut(s) 1054
ApeKI GCWGC 5 cut(s) 257, 566, 656, 821, 982
ApoI RAATTY 4 cut(s) 629, 966, 1024, 1100
AspS9I GGNCC 2 cut(s) 365, 607
AsuC2I CCSGG 1 cut(s) 719
AsuHPI GGTGA 6 cut(s) 158, 428, 520, 698, 777, 1061
AsuII TTCGAA 2 cut(s) 964, 1104
AvaII GGWCC 2 cut(s) 365, 607
BbvCI CCTCAGC 1 cut(s) 562
BbvI GCAGC 5 cut(s) 244, 578, 643, 808, 969
BccI CCATC 5 cut(s) 65, 113, 137, 529, 638
BciT130I CCWGG 1 cut(s) 99
BcnI CCSGG 1 cut(s) 719
BcoDI GTCTC 3 cut(s) 544, 1085, 1115
BfaI CTAG 5 cut(s) 15, 119, 498, 761, 1077
BisI GCNGC 5 cut(s) 258, 567, 657, 822, 983
BlsI GCNGC 5 cut(s) 259, 568, 658, 823, 984
Bme1390I CCNGG 2 cut(s) 99, 719
Bme18I GGWCC 2 cut(s) 365, 607
BmgT120I GGNCC 2 cut(s) 365, 607
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 2 cut(s) 99, 719
BmsI GCATC 4 cut(s) 142, 520, 799, 808
BpmI CTGGAG 2 cut(s) 213, 464
Bpu10I CCTNAGC 1 cut(s) 562
Bpu14I TTCGAA 2 cut(s) 964, 1104
BpuMI CCSGG 1 cut(s) 719
Bsa29I ATCGAT 1 cut(s) 642
BsaJI CCNNGG 2 cut(s) 97, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 925, 946
Bse1I ACTGG 1 cut(s) 784
Bse3DI GCAATG 1 cut(s) 552
BseBI CCWGG 1 cut(s) 99
BseCI ATCGAT 1 cut(s) 642
BseDI CCNNGG 2 cut(s) 97, 603
BseGI GGATG 4 cut(s) 105, 148, 157, 979
BseLI CCNNNNNNNGG 3 cut(s) 41, 925, 946
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 4 cut(s) 117, 279, 555, 576
BseNI ACTGG 1 cut(s) 784
BseXI GCAGC 5 cut(s) 244, 578, 643, 808, 969
BshFI GGCC 1 cut(s) 1056
BshVI ATCGAT 1 cut(s) 642
BsiSI CCGG 1 cut(s) 719
BslFI GGGAC 1 cut(s) 1004
BslI CCNNNNNNNGG 3 cut(s) 41, 925, 946
BsmAI GTCTC 3 cut(s) 544, 1085, 1115
BsmFI GGGAC 1 cut(s) 1004
BsmI GAATGC 1 cut(s) 18
BsnI GGCC 1 cut(s) 1056
Bsp119I TTCGAA 2 cut(s) 964, 1104
Bsp143I GATC 3 cut(s) 291, 870, 910
BspACI CCGC 1 cut(s) 855
BspANI GGCC 1 cut(s) 1056
BspCNI CTCAG 4 cut(s) 118, 280, 554, 575
BspDI ATCGAT 1 cut(s) 642
BspHI TCATGA 1 cut(s) 1117
BspLI GGNNCC 1 cut(s) 103
BspPI GGATC 1 cut(s) 286
BspT104I TTCGAA 2 cut(s) 964, 1104
BsrDI GCAATG 1 cut(s) 552
BsrI ACTGG 1 cut(s) 784
BssECI CCNNGG 2 cut(s) 97, 603
BssMI GATC 3 cut(s) 291, 870, 910
BssT1I CCWWGG 1 cut(s) 603
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 2 cut(s) 58, 245
Bst6I CTCTTC 1 cut(s) 1161
BstAPI GCANNNNNTGC 2 cut(s) 161, 818
BstBI TTCGAA 2 cut(s) 964, 1104
BstDEI CTNAG 6 cut(s) 62, 126, 288, 535, 541, 562
BstF5I GGATG 4 cut(s) 105, 148, 157, 979
BstKTI GATC 3 cut(s) 294, 873, 913
BstMAI GTCTC 3 cut(s) 544, 1085, 1115
BstMBI GATC 3 cut(s) 291, 870, 910
BstMWI GCNNNNNNNGC 4 cut(s) 136, 161, 563, 818
BstNI CCWGG 1 cut(s) 99
BstSCI CCNGG 2 cut(s) 97, 717
BstV1I GCAGC 5 cut(s) 244, 578, 643, 808, 969
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
Bsu15I ATCGAT 1 cut(s) 642
BsuRI GGCC 1 cut(s) 1056
BsuTUI ATCGAT 1 cut(s) 642
BtsCI GGATG 4 cut(s) 105, 148, 157, 979
BtsIMutI CAGTG 1 cut(s) 54
CaiI CAGNNNCTG 1 cut(s) 139
CciI TCATGA 1 cut(s) 1117
Cfr13I GGNCC 2 cut(s) 365, 607
ClaI ATCGAT 1 cut(s) 642
Csp6I GTAC 3 cut(s) 195, 373, 1209
CviAII CATG 4 cut(s) 217, 815, 986, 1118
CviQI GTAC 3 cut(s) 195, 373, 1209
DdeI CTNAG 6 cut(s) 62, 126, 288, 535, 541, 562
DpnI GATC 3 cut(s) 293, 872, 912
DpnII GATC 3 cut(s) 291, 870, 910
Eam1104I CTCTTC 1 cut(s) 1161
EarI CTCTTC 1 cut(s) 1161
Eco130I CCWWGG 1 cut(s) 603
Eco147I AGGCCT 1 cut(s) 1056
Eco32I GATATC 1 cut(s) 758
Eco47I GGWCC 2 cut(s) 365, 607
Eco57I CTGAAG 3 cut(s) 11, 116, 1185
EcoRI GAATTC 2 cut(s) 629, 966
EcoRII CCWGG 1 cut(s) 97
EcoRV GATATC 1 cut(s) 758
EcoT14I CCWWGG 1 cut(s) 603
EcoT22I ATGCAT 1 cut(s) 554
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 4 cut(s) 220, 818, 989, 1121
FalI AAGNNNNNCTT 2 cut(s) 1122, 1154
FaqI GGGAC 1 cut(s) 1004
FatI CATG 4 cut(s) 216, 814, 985, 1117
FauNDI CATATG 1 cut(s) 548
Fnu4HI GCNGC 5 cut(s) 258, 567, 657, 822, 983
FokI GGATG 4 cut(s) 92, 155, 164, 986
Fsp4HI GCNGC 5 cut(s) 258, 567, 657, 822, 983
FspBI CTAG 5 cut(s) 15, 119, 498, 761, 1077
GluI GCNGC 5 cut(s) 258, 567, 657, 822, 983
GsuI CTGGAG 2 cut(s) 213, 464
HaeIII GGCC 1 cut(s) 1056
HapII CCGG 1 cut(s) 719
Hin1II CATG 4 cut(s) 220, 818, 989, 1121
HincII GTYRAC 1 cut(s) 849
HindII GTYRAC 1 cut(s) 849
HindIII AAGCTT 2 cut(s) 410, 1107
HinfI GANTC 2 cut(s) 122, 475
HpaI GTTAAC 1 cut(s) 849
HpaII CCGG 1 cut(s) 719
HphI GGTGA 6 cut(s) 158, 428, 520, 698, 777, 1061
Hpy166II GTNNAC 1 cut(s) 849
Hpy188I TCNGA 7 cut(s) 127, 135, 544, 669, 915, 949, 1165
Hpy188III TCNNGA 4 cut(s) 186, 230, 971, 1118
Hpy8I GTNNAC 1 cut(s) 849
HpyAV CCTTC 4 cut(s) 35, 950, 971, 1046
HpyCH4III ACNGT 2 cut(s) 58, 245
HpyCH4IV ACGT 1 cut(s) 993
HpyF10VI GCNNNNNNNGC 4 cut(s) 136, 161, 563, 818
HpyF3I CTNAG 6 cut(s) 62, 126, 288, 535, 541, 562
HpySE526I ACGT 1 cut(s) 993
Hsp92II CATG 4 cut(s) 220, 818, 989, 1121
KspAI GTTAAC 1 cut(s) 849
Kzo9I GATC 3 cut(s) 291, 870, 910
LmnI GCTCC 1 cut(s) 432
Lsp1109I GCAGC 5 cut(s) 244, 578, 643, 808, 969
LweI GCATC 4 cut(s) 142, 520, 799, 808
MaeI CTAG 5 cut(s) 15, 119, 498, 761, 1077
MaeII ACGT 1 cut(s) 993
MaeIII GTNAC 3 cut(s) 208, 691, 887
MalI GATC 3 cut(s) 293, 872, 912
MboI GATC 3 cut(s) 291, 870, 910
MboII GAAGA 7 cut(s) 295, 355, 572, 990, 1033, 1142, 1178
MflI RGATCY 1 cut(s) 291
MluCI AATT 8 cut(s) 629, 797, 841, 955, 966, 1024, 1084, 1100
MmeI TCCRAC 1 cut(s) 692
MnlI CCTC 5 cut(s) 80, 142, 499, 571, 851
Mph1103I ATGCAT 1 cut(s) 554
MseI TTAA 2 cut(s) 848, 1083
MslI CAYNNNNRTG 1 cut(s) 894
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 1 cut(s) 719
MspR9I CCNGG 2 cut(s) 99, 719
Mva1269I GAATGC 1 cut(s) 18
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 4 cut(s) 136, 161, 563, 818
NciI CCSGG 1 cut(s) 719
NdeI CATATG 1 cut(s) 548
NdeII GATC 3 cut(s) 291, 870, 910
NlaIII CATG 4 cut(s) 220, 818, 989, 1121
NlaIV GGNNCC 1 cut(s) 103
NmuCI GTSAC 3 cut(s) 208, 691, 887
NsiI ATGCAT 1 cut(s) 554
NspV TTCGAA 2 cut(s) 964, 1104
OliI CACNNNNGTG 1 cut(s) 894
PagI TCATGA 1 cut(s) 1117
PceI AGGCCT 1 cut(s) 1056
PctI GAATGC 1 cut(s) 18
PfeI GAWTC 2 cut(s) 122, 475
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 5 cut(s) 259, 568, 658, 823, 984
PsiI TTATAA 1 cut(s) 1142
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 103
PspPI GGNCC 2 cut(s) 365, 607
PstNI CAGNNNCTG 1 cut(s) 139
PsuI RGATCY 1 cut(s) 291
RsaI GTAC 3 cut(s) 196, 374, 1210
RsaNI GTAC 3 cut(s) 195, 373, 1209
RseI CAYNNNNRTG 1 cut(s) 894
SaqAI TTAA 2 cut(s) 848, 1083
SatI GCNGC 5 cut(s) 258, 567, 657, 822, 983
Sau3AI GATC 3 cut(s) 291, 870, 910
Sau96I GGNCC 2 cut(s) 365, 607
ScrFI CCNGG 2 cut(s) 99, 719
SfaNI GCATC 4 cut(s) 142, 520, 799, 808
SfuI TTCGAA 2 cut(s) 964, 1104
SinI GGWCC 2 cut(s) 365, 607
SmiMI CAYNNNNRTG 1 cut(s) 894
Sse9I AATT 8 cut(s) 629, 797, 841, 955, 966, 1024, 1084, 1100
SseBI AGGCCT 1 cut(s) 1056
SsiI CCGC 1 cut(s) 855
SspMI CTAG 5 cut(s) 15, 119, 498, 761, 1077
StuI AGGCCT 1 cut(s) 1056
StyD4I CCNGG 2 cut(s) 97, 717
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 2 cut(s) 58, 245
TaiI ACGT 1 cut(s) 996
TaqI TCGA 4 cut(s) 642, 863, 964, 1104
TasI AATT 8 cut(s) 629, 797, 841, 955, 966, 1024, 1084, 1100
TatI WGTACW 3 cut(s) 194, 372, 1208
TfiI GAWTC 2 cut(s) 122, 475
Tru1I TTAA 2 cut(s) 848, 1083
Tru9I TTAA 2 cut(s) 848, 1083
TscAI CASTG 1 cut(s) 61
TseFI GTSAC 3 cut(s) 208, 691, 887
TseI GCWGC 5 cut(s) 257, 566, 656, 821, 982
Tsp45I GTSAC 3 cut(s) 208, 691, 887
TspDTI ATGAA 1 cut(s) 1143
TspGWI ACGGA 1 cut(s) 630
TspRI CASTG 1 cut(s) 61
Van91I CCANNNNNTGG 1 cut(s) 41
VpaK11BI GGWCC 2 cut(s) 365, 607
XapI RAATTY 4 cut(s) 629, 966, 1024, 1100
XspI CTAG 5 cut(s) 15, 119, 498, 761, 1077
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.