pycom08g08700

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000265
Physical Location & Seq
Reverse (-)
7014611 .. 7016209
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08700.1

Sequence Viewer

Length: 1149 bp
ATGGAAAGAAGCGGTAGAGTAGAACCCCCCACAGTCTTTGGAAAGATAGTCACAGTTCTCAGTATCGATGGGGGCGGAATAAGAGGCCTCATTCCGGCAACCATTCTTACTTTCCTCGAATCTGAACTTCAGAAGCTGGATGGTGAAGATGCAAGGATCGCTGACTATTTTGATGTGATTGCAGGAACAAGCACAGGTGGTCTTGTGACTGCTATGCTCGCAGCCCCAAACGAGAATAACCGCCCACTGTTCGCTGCCAAAGATATTATAGACTTCTACTTAAATCAATGTCCTAAAATCTTCCCCCAAAACACTTGGCCAATTTTTCCTAATACGACAAAGATTATCAAAGCTCTAGCAGGACCAAAATACAATGGCAAGTATTTGCATCGCTTGATTAGGGAAAGGCTGGGCAACAAAAAACTGCACGACACATTGACTAATGTTGTAATTCCCACATTTGACATCAAGAATCTCCATACAACTATCTTCTCCAAGTTTAAGGTGAAAAAGAAGCCATCATACGATGCCTTACTCTCTGACATATGCATTGGAACCTCAGCGGCACCAACTTATCTTCCAGCTCATTATTTTGAAACCAAGAACGCTGCAGGCAGAACTAGGGAATTCAACCTTATAGATGGTGGCGTGGCTGCAAATAATCCGGTATATGTGATAGGAGGAGTTATATTACCAGATTGTCAATTTATACCATTGATTTTAAAAACTTTACTTGCTGTTTGTGAAGTTACAAACGAAATAAGGAAGGTGAATCCAGACATCTTTCCTGCGAAACCTATGGACTATGGGAGATTTCTAGTGATATCATTAGGAACTGGTTCATCAAAAGCTGAAATGAAATACCATGCACCCTCTACAGCTAAATGGGGCCTGTTGAATTGGTTAACCAGCGGTGGCTCCACCCCTATCATTAATGTTTTCAGTCAAGCAAGCAACGATATGGTTGATCTTCACCTTTCTGCAATTTTCCAAGCCCTTCATTCTGAAGAAAATTATCTTAGAATTCAGGTACTGAACTCATCATCCGAAATGCATTTTATCCATAGATTCATCAGTATAACTCTTTTTAGAATAATGTTGTTTAGACACAAAAAACTGACATCATTTCTGATACCAAATGTAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

383

Amino Acids

42.37

Weight (kDa)

9.25

Isoelectric Point (pI)

25.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 20 - 224 6e-25 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 565
AciI CCGC 5 cut(s) 12, 75, 241, 563, 912
AclWI GGATC 1 cut(s) 164
AcoI YGGCCR 1 cut(s) 317
AcsI RAATTY 2 cut(s) 626, 1023
AcuI CTGAAG 2 cut(s) 113, 1026
AfaI GTAC 1 cut(s) 1032
AfiI CCNNNNNNNGG 2 cut(s) 94, 1142
AgsI TTSAA 3 cut(s) 596, 631, 898
AloI GAACNNNNNNTCC 2 cut(s) 1028, 1060
AluBI AGCT 5 cut(s) 136, 353, 584, 851, 881
AluI AGCT 5 cut(s) 136, 353, 584, 851, 881
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 2 cut(s) 136, 1033
AoxI GGCC 3 cut(s) 85, 317, 889
ApeKI GCWGC 4 cut(s) 221, 254, 608, 653
ApoI RAATTY 2 cut(s) 626, 1023
AseI ATTAAT 1 cut(s) 933
Asp700I GAANNNNTTC 1 cut(s) 838
AspS9I GGNCC 2 cut(s) 362, 889
AsuHPI GGTGA 4 cut(s) 155, 517, 781, 965
AvaII GGWCC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 319
BanI GGYRCC 1 cut(s) 565
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BbvCI CCTCAGC 1 cut(s) 559
BbvI GCAGC 4 cut(s) 233, 241, 595, 640
BccI CCATC 4 cut(s) 62, 134, 526, 635
BfaI CTAG 3 cut(s) 356, 621, 818
BfmI CTRYAG 2 cut(s) 609, 876
BisI GCNGC 5 cut(s) 222, 255, 564, 609, 654
BlsI GCNGC 5 cut(s) 223, 256, 565, 610, 655
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 2 cut(s) 362, 889
BmiI GGNNCC 4 cut(s) 556, 567, 890, 919
BmsI GCATC 3 cut(s) 139, 397, 517
Bpu10I CCTNAGC 1 cut(s) 559
Bsa29I ATCGAT 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 1142
Bse1I ACTGG 1 cut(s) 841
BseCI ATCGAT 1 cut(s) 66
BseGI GGATG 2 cut(s) 145, 1043
BseLI CCNNNNNNNGG 2 cut(s) 94, 1142
BseMII CTCAG 2 cut(s) 73, 573
BseNI ACTGG 1 cut(s) 841
BseRI GAGGAG 1 cut(s) 696
BseXI GCAGC 4 cut(s) 233, 241, 595, 640
BseYI CCCAGC 1 cut(s) 409
BsgI GTGCAG 1 cut(s) 410
BshFI GGCC 3 cut(s) 87, 319, 891
BshNI GGYRCC 1 cut(s) 565
BshVI ATCGAT 1 cut(s) 66
BsiSI CCGG 2 cut(s) 95, 665
BslI CCNNNNNNNGG 2 cut(s) 94, 1142
BsnI GGCC 3 cut(s) 87, 319, 891
Bsp143I GATC 2 cut(s) 156, 967
BspACI CCGC 5 cut(s) 12, 75, 241, 563, 912
BspANI GGCC 3 cut(s) 87, 319, 891
BspCNI CTCAG 2 cut(s) 72, 572
BspDI ATCGAT 1 cut(s) 66
BspLI GGNNCC 4 cut(s) 556, 567, 890, 919
BspMAI CTGCAG 1 cut(s) 613
BspPI GGATC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 565
BsrI ACTGG 1 cut(s) 841
BssMI GATC 2 cut(s) 156, 967
Bst4CI ACNGT 3 cut(s) 34, 55, 249
BstC8I GCNNGC 3 cut(s) 219, 613, 952
BstDEI CTNAG 3 cut(s) 59, 559, 1019
BstF5I GGATG 2 cut(s) 145, 1043
BstKTI GATC 2 cut(s) 159, 970
BstMBI GATC 2 cut(s) 156, 967
BstMWI GCNNNNNNNGC 2 cut(s) 158, 218
BstSFI CTRYAG 2 cut(s) 609, 876
BstV1I GCAGC 4 cut(s) 233, 241, 595, 640
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 3 cut(s) 87, 319, 891
BsuTUI ATCGAT 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 374
BtsCI GGATG 2 cut(s) 145, 1043
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 3 cut(s) 219, 613, 952
CaiI CAGNNNCTG 2 cut(s) 136, 1033
Cfr13I GGNCC 2 cut(s) 362, 889
ClaI ATCGAT 1 cut(s) 66
Csp6I GTAC 1 cut(s) 1031
CspCI CAANNNNNGTGG 2 cut(s) 19, 54
CviAII CATG 1 cut(s) 866
CviQI GTAC 1 cut(s) 1031
DdeI CTNAG 3 cut(s) 59, 559, 1019
DpnI GATC 2 cut(s) 158, 969
DpnII GATC 2 cut(s) 156, 967
DraI TTTAAA 1 cut(s) 723
EaeI YGGCCR 1 cut(s) 317
EciI GGCGGA 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 87
Eco32I GATATC 1 cut(s) 825
Eco47I GGWCC 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 113, 1026
EcoO109I RGGNCCY 1 cut(s) 889
EcoRI GAATTC 2 cut(s) 626, 1023
EcoRV GATATC 1 cut(s) 825
EcoT22I ATGCAT 2 cut(s) 551, 1056
FaeI CATG 1 cut(s) 869
FatI CATG 1 cut(s) 865
FauNDI CATATG 1 cut(s) 545
Fnu4HI GCNGC 5 cut(s) 222, 255, 564, 609, 654
FokI GGATG 2 cut(s) 152, 1030
Fsp4HI GCNGC 5 cut(s) 222, 255, 564, 609, 654
FspBI CTAG 3 cut(s) 356, 621, 818
GluI GCNGC 5 cut(s) 222, 255, 564, 609, 654
GsaI CCCAGC 1 cut(s) 413
HaeIII GGCC 3 cut(s) 87, 319, 891
HapII CCGG 2 cut(s) 95, 665
Hin1II CATG 1 cut(s) 869
HincII GTYRAC 1 cut(s) 906
HindII GTYRAC 1 cut(s) 906
HinfI GANTC 4 cut(s) 119, 472, 772, 1068
HpaI GTTAAC 1 cut(s) 906
HpaII CCGG 2 cut(s) 95, 665
HphI GGTGA 4 cut(s) 155, 517, 781, 965
Hpy166II GTNNAC 1 cut(s) 906
Hpy188I TCNGA 6 cut(s) 124, 132, 541, 1006, 1048, 1131
Hpy188III TCNNGA 2 cut(s) 469, 776
Hpy8I GTNNAC 1 cut(s) 906
HpyAV CCTTC 2 cut(s) 760, 1007
HpyCH4III ACNGT 3 cut(s) 34, 55, 249
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 218
HpyF3I CTNAG 3 cut(s) 59, 559, 1019
Hsp92II CATG 1 cut(s) 869
KspAI GTTAAC 1 cut(s) 906
Kzo9I GATC 2 cut(s) 156, 967
LmnI GCTCC 1 cut(s) 923
Lsp1109I GCAGC 4 cut(s) 233, 241, 595, 640
LweI GCATC 3 cut(s) 139, 397, 517
MaeI CTAG 3 cut(s) 356, 621, 818
MaeIII GTNAC 3 cut(s) 49, 205, 748
MalI GATC 2 cut(s) 158, 969
MboI GATC 2 cut(s) 156, 967
MboII GAAGA 6 cut(s) 158, 292, 481, 569, 962, 1019
MlsI TGGCCA 1 cut(s) 319
MluCI AATT 8 cut(s) 321, 450, 626, 704, 898, 984, 1012, 1023
MluNI TGGCCA 1 cut(s) 319
MnlI CCTC 6 cut(s) 77, 98, 125, 568, 674, 883
Mox20I TGGCCA 1 cut(s) 319
Mph1103I ATGCAT 2 cut(s) 551, 1056
MroXI GAANNNNTTC 1 cut(s) 838
MscI TGGCCA 1 cut(s) 319
MseI TTAA 5 cut(s) 281, 501, 722, 905, 933
Msp20I TGGCCA 1 cut(s) 319
MspA1I CMGCKG 2 cut(s) 563, 912
MspI CCGG 2 cut(s) 95, 665
MwoI GCNNNNNNNGC 2 cut(s) 158, 218
NdeI CATATG 1 cut(s) 545
NdeII GATC 2 cut(s) 156, 967
NlaIII CATG 1 cut(s) 869
NlaIV GGNNCC 4 cut(s) 556, 567, 890, 919
NmuCI GTSAC 2 cut(s) 49, 205
NsiI ATGCAT 2 cut(s) 551, 1056
PceI AGGCCT 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 838
PfeI GAWTC 4 cut(s) 119, 472, 772, 1068
PkrI GCNGC 5 cut(s) 223, 256, 565, 610, 655
PshBI ATTAAT 1 cut(s) 933
PspFI CCCAGC 1 cut(s) 409
PspN4I GGNNCC 4 cut(s) 556, 567, 890, 919
PspPI GGNCC 2 cut(s) 362, 889
PstI CTGCAG 1 cut(s) 613
PstNI CAGNNNCTG 2 cut(s) 136, 1033
RsaI GTAC 1 cut(s) 1032
RsaNI GTAC 1 cut(s) 1031
SaqAI TTAA 5 cut(s) 281, 501, 722, 905, 933
SatI GCNGC 5 cut(s) 222, 255, 564, 609, 654
Sau3AI GATC 2 cut(s) 156, 967
Sau96I GGNCC 2 cut(s) 362, 889
SfaNI GCATC 3 cut(s) 139, 397, 517
SfcI CTRYAG 2 cut(s) 609, 876
SinI GGWCC 1 cut(s) 362
Sse9I AATT 8 cut(s) 321, 450, 626, 704, 898, 984, 1012, 1023
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 5 cut(s) 12, 75, 241, 563, 912
SspMI CTAG 3 cut(s) 356, 621, 818
StuI AGGCCT 1 cut(s) 87
TaaI ACNGT 3 cut(s) 34, 55, 249
TaqI TCGA 2 cut(s) 66, 117
TasI AATT 8 cut(s) 321, 450, 626, 704, 898, 984, 1012, 1023
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 4 cut(s) 119, 472, 772, 1068
Tru1I TTAA 5 cut(s) 281, 501, 722, 905, 933
Tru9I TTAA 5 cut(s) 281, 501, 722, 905, 933
TscAI CASTG 1 cut(s) 252
TseFI GTSAC 2 cut(s) 49, 205
TseI GCWGC 4 cut(s) 221, 254, 608, 653
Tsp45I GTSAC 2 cut(s) 49, 205
TspDTI ATGAA 4 cut(s) 831, 872, 989, 1060
TspRI CASTG 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 362
VspI ATTAAT 1 cut(s) 933
XapI RAATTY 2 cut(s) 626, 1023
XmnI GAANNNNTTC 1 cut(s) 838
XspI CTAG 3 cut(s) 356, 621, 818
Zsp2I ATGCAT 2 cut(s) 551, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.