Rw3G022650

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000265
Physical Location & Seq
Forward (+)
27908673 .. 27910175
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022650.1

Sequence Viewer

Length: 717 bp
ATGGCATTACAGGCCCCAACCTACGGAAACCTAGTCACTGTTCTGAGCATCGATGGCGGTGGAATAAGAGGCCAAATCCCAGGAACCAGCCAGCTATCTTTTCCAGCTTCAAGCGGCACCAACTTATCTTCCAGTCATTATTTTGAAACCAACGGCCTAAAAGGAAAAGTTAGAGAATTTCACCTGATAGATGGTGGATTGGCAGCAAATAATCCGACTTTGATTGCTATTGGTGAAGTGACAAAGGAAATAGTTAAGGGGAGTTCAGACTTCTTTCCTATAAAACCAATGGACTATGGACGATTTCTGGTGATATCCTTAGGAACTGGCTCATCGAAAGCTGAACTGAAATACAATGCTCATGTTGCCTCCAAGTGGGGTGTGTGGAATTGGTTAACGAGCCGTGGTTCCACCCCATTGATCAATGCTTTCAGTCAAGCAAGTGCCGATATGGTCGATTTGCATCTTTCTGTGGTGTTCAAAGCCCTTCACTCGGAGAAAAACTATCTTCGAATTCAGGATCACACACTAAGTGGTGAAGTATCTTCAGTAGATGTAGCAACAGAGAAGAATCTGGACCATCTTCTCAAAGTTGGTGAAGGACTATTGAGACGACCAGTTTCAAGGGTTAATTTAGAGACAGGCAGATTCGAAGCTTGTAATCATGAGACTAATAAAGAGGCTCTTATAAACATGTTTGAAAACCCTAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

238

Amino Acids

25.83

Weight (kDa)

6.71

Isoelectric Point (pI)

21.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
AccB1I GGYRCC 1 cut(s) 116
AciI CCGC 2 cut(s) 57, 114
AclWI GGATC 1 cut(s) 528
AcsI RAATTY 2 cut(s) 176, 513
AcuI CTGAAG 1 cut(s) 531
AdeI CACNNNGTG 1 cut(s) 533
AfiI CCNNNNNNNGG 3 cut(s) 23, 375, 493
AflIII ACRYGT 1 cut(s) 693
AgsI TTSAA 5 cut(s) 111, 146, 481, 624, 701
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 4 cut(s) 94, 107, 341, 656
AluI AGCT 4 cut(s) 94, 107, 341, 656
Alw26I GTCTC 3 cut(s) 604, 632, 662
AlwI GGATC 1 cut(s) 528
AoxI GGCC 3 cut(s) 12, 70, 154
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 2 cut(s) 176, 513
AspS9I GGNCC 2 cut(s) 13, 577
AsuHPI GGTGA 5 cut(s) 173, 245, 322, 548, 608
AsuII TTCGAA 2 cut(s) 511, 651
AvaII GGWCC 1 cut(s) 577
AxyI CCTNAGG 1 cut(s) 319
BanI GGYRCC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 215
BccI CCATC 3 cut(s) 47, 185, 588
BceAI ACGGC 2 cut(s) 169, 387
BciT130I CCWGG 1 cut(s) 81
BclI TGATCA 1 cut(s) 420
BcoDI GTCTC 3 cut(s) 604, 632, 662
BfaI CTAG 1 cut(s) 32
BisI GCNGC 2 cut(s) 115, 204
BlsI GCNGC 2 cut(s) 116, 205
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 577
BmgT120I GGNCC 2 cut(s) 13, 577
BmiI GGNNCC 4 cut(s) 15, 85, 118, 409
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 57, 472
Bpu14I TTCGAA 2 cut(s) 511, 651
Bsa29I ATCGAT 1 cut(s) 51
BsaBI GATNNNNATC 1 cut(s) 462
BsaJI CCNNGG 2 cut(s) 79, 403
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 375, 493
Bse1I ACTGG 3 cut(s) 132, 331, 617
Bse21I CCTNAGG 1 cut(s) 319
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 1 cut(s) 81
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 79, 403
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 3 cut(s) 23, 375, 493
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 3 cut(s) 132, 331, 617
BseXI GCAGC 1 cut(s) 215
BshFI GGCC 3 cut(s) 14, 72, 156
BshNI GGYRCC 1 cut(s) 116
BshVI ATCGAT 1 cut(s) 51
BslI CCNNNNNNNGG 3 cut(s) 23, 375, 493
BsmAI GTCTC 3 cut(s) 604, 632, 662
BsmBI CGTCTC 1 cut(s) 604
BsnI GGCC 3 cut(s) 14, 72, 156
Bsp119I TTCGAA 2 cut(s) 511, 651
Bsp143I GATC 2 cut(s) 420, 520
BspACI CCGC 2 cut(s) 57, 114
BspANI GGCC 3 cut(s) 14, 72, 156
BspCNI CTCAG 1 cut(s) 36
BspDI ATCGAT 1 cut(s) 51
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 4 cut(s) 15, 85, 118, 409
BspPI GGATC 1 cut(s) 528
BspT104I TTCGAA 2 cut(s) 511, 651
BspT107I GGYRCC 1 cut(s) 116
BsrI ACTGG 3 cut(s) 132, 331, 617
BssECI CCNNGG 2 cut(s) 79, 403
BssMI GATC 2 cut(s) 420, 520
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 40
BstBI TTCGAA 2 cut(s) 511, 651
BstC8I GCNNGC 1 cut(s) 92
BstDEI CTNAG 3 cut(s) 44, 319, 530
BstDSI CCRYGG 1 cut(s) 403
BstKTI GATC 2 cut(s) 423, 523
BstMAI GTCTC 3 cut(s) 604, 632, 662
BstMBI GATC 2 cut(s) 420, 520
BstMWI GCNNNNNNNGC 3 cut(s) 11, 54, 365
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 1 cut(s) 697
BstSCI CCNGG 1 cut(s) 79
BstV1I GCAGC 1 cut(s) 215
Bsu15I ATCGAT 1 cut(s) 51
Bsu36I CCTNAGG 1 cut(s) 319
BsuRI GGCC 3 cut(s) 14, 72, 156
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 36
Cac8I GCNNGC 1 cut(s) 92
CciI TCATGA 1 cut(s) 664
Cfr13I GGNCC 2 cut(s) 13, 577
ClaI ATCGAT 1 cut(s) 51
CviAII CATG 3 cut(s) 362, 665, 694
DdeI CTNAG 3 cut(s) 44, 319, 530
DpnI GATC 2 cut(s) 422, 522
DpnII GATC 2 cut(s) 420, 520
DraIII CACNNNGTG 1 cut(s) 533
Eco32I GATATC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 577
Eco57I CTGAAG 1 cut(s) 531
Eco81I CCTNAGG 1 cut(s) 319
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 79
EcoRV GATATC 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 604
FaeI CATG 3 cut(s) 365, 668, 697
FaiI YATR 7 cut(s) 281, 297, 363, 452, 666, 689, 695
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FatI CATG 3 cut(s) 361, 664, 693
FbaI TGATCA 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 115, 204
Fsp4HI GCNGC 2 cut(s) 115, 204
FspBI CTAG 1 cut(s) 32
GluI GCNGC 2 cut(s) 115, 204
HaeIII GGCC 3 cut(s) 14, 72, 156
Hin1II CATG 3 cut(s) 365, 668, 697
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 2 cut(s) 571, 648
HpaI GTTAAC 1 cut(s) 396
HphI GGTGA 5 cut(s) 173, 245, 322, 548, 608
Hpy166II GTNNAC 1 cut(s) 396
Hpy188I TCNGA 4 cut(s) 45, 216, 268, 496
Hpy188III TCNNGA 3 cut(s) 518, 575, 665
Hpy8I GTNNAC 1 cut(s) 396
HpyAV CCTTC 2 cut(s) 497, 593
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4V TGCA 1 cut(s) 463
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 54, 365
HpyF3I CTNAG 3 cut(s) 44, 319, 530
Hsp92II CATG 3 cut(s) 365, 668, 697
Ksp22I TGATCA 1 cut(s) 420
KspAI GTTAAC 1 cut(s) 396
Kzo9I GATC 2 cut(s) 420, 520
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 2 cut(s) 57, 472
MaeI CTAG 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 34, 238
MalI GATC 2 cut(s) 422, 522
MboI GATC 2 cut(s) 420, 520
MboII GAAGA 5 cut(s) 120, 500, 537, 575, 580
MluCI AATT 5 cut(s) 176, 388, 513, 631, 709
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 3 cut(s) 62, 379, 673
MseI TTAA 3 cut(s) 255, 395, 630
MspR9I CCNGG 1 cut(s) 81
MvaI CCWGG 1 cut(s) 81
MwoI GCNNNNNNNGC 3 cut(s) 11, 54, 365
NdeII GATC 2 cut(s) 420, 520
NlaIII CATG 3 cut(s) 365, 668, 697
NlaIV GGNNCC 4 cut(s) 15, 85, 118, 409
NmuCI GTSAC 2 cut(s) 34, 238
NspI RCATGY 1 cut(s) 697
NspV TTCGAA 2 cut(s) 511, 651
PagI TCATGA 1 cut(s) 664
PciI ACATGT 1 cut(s) 693
PfeI GAWTC 2 cut(s) 571, 648
PkrI GCNGC 2 cut(s) 116, 205
PscI ACATGT 1 cut(s) 693
PsiI TTATAA 1 cut(s) 689
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 4 cut(s) 15, 85, 118, 409
PspPI GGNCC 2 cut(s) 13, 577
SaqAI TTAA 3 cut(s) 255, 395, 630
SatI GCNGC 2 cut(s) 115, 204
Sau3AI GATC 2 cut(s) 420, 520
Sau96I GGNCC 2 cut(s) 13, 577
ScrFI CCNGG 1 cut(s) 81
SetI ASST 7 cut(s) 23, 33, 96, 109, 186, 343, 658
SfaNI GCATC 2 cut(s) 57, 472
SfuI TTCGAA 2 cut(s) 511, 651
SinI GGWCC 1 cut(s) 577
Sse9I AATT 5 cut(s) 176, 388, 513, 631, 709
SsiI CCGC 2 cut(s) 57, 114
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 79
TaaI ACNGT 1 cut(s) 40
TaqI TCGA 5 cut(s) 51, 335, 456, 511, 651
TasI AATT 5 cut(s) 176, 388, 513, 631, 709
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 2 cut(s) 571, 648
Tru1I TTAA 3 cut(s) 255, 395, 630
Tru9I TTAA 3 cut(s) 255, 395, 630
TscAI CASTG 1 cut(s) 43
TseFI GTSAC 2 cut(s) 34, 238
TseI GCWGC 1 cut(s) 203
Tsp45I GTSAC 2 cut(s) 34, 238
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 577
XapI RAATTY 2 cut(s) 176, 513
XceI RCATGY 1 cut(s) 697
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.