RLG00000010721

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000265
Physical Location & Seq
Reverse (-)
3838806 .. 3840808
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010721

Sequence Viewer

Length: 1047 bp
ATGGAAAGAACCAAGAGTATACCTCTACAGCCCCCAACCTACGGAAACCTAGTAACTATTTTGAGCATTGATGGTGGTGGAATAAGAGGCCTTATCCCAGCAGCCATCCTTGATTACCTTGAATCCGAGCTTCAGAAGCTAGATGGCAAGGATGCAAGACTAGCAGATTATTTTGATGTGATTGCCGGGACTAGCACAGGTGGTCTCGTAACTGCCATGATTGCAACCCCAGACGAGAACAACAACAACCGCCCTGTGTTTGCCGCAAAAGATATTAAGGATTTCTATCTTACACACTGCCCTAAAATCTTCCCTCAGAGGCACTGTCCAGTGTTTCCTTTTACTGTAAAGATTATCAAAGCTTTAGCAGGACCAAAATATGATGGAAAATATCTCCACCACTTGGTTGGGGAAAAATTAGGTGACAAAAAACTGCACAAGACATTGACTAATGTGGTCATTCCCACTTTTGACATAAAACAACTCCAGCCAACTATCTTTTCCAGCTTTGAGGTGAAAAGTAAGCCATGCTCCGATGCTCTGCTCAGAGATATATGCATTGCAACCTCAGCTGCACCTACTTATCTCCCAGCTCATTATTTTGAAACCCAAGACTTCCAAGGCAACGTTAAGGAATTTAACCTTATTGATGGCGGCGTTTGTGCAAATAACCCGACTTTAGTTGCCATTGGTGAAGTGACAAAGATGATACTCAAGGGCAGTTCAGACTTCTTTCCTATTAAACCCATGGACTATGGTCGATTTCTAGTCATATCAATAGGAACTGGCTCACCCAAGATAGAACTGAAATATCATGCCCATGCTGCTGCCAAATGGGGTTTGTTGGATTGGTTAACCAGCGGTGGACATACCCCATTAATTGATGTTTTCAGTCAGTCAAGTTCTGATATGGTCGATTTTAACCTGTCGGTTGTTTTCCAAGCCCTACACTCGGAGGAAAACTATCTTCGAATTCAGGTATTTCACGCGGCTGCTATTAATATACATTCTAAATTTATCATCTTGCACTACAAAATTAATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

349

Amino Acids

38.45

Weight (kDa)

7.21

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 21 - 230 2.7e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 403
AccI GTMKAC 1 cut(s) 19
AccII CGCG 1 cut(s) 989
AciI CCGC 5 cut(s) 250, 264, 654, 861, 989
AclI AACGTT 1 cut(s) 627
AcsI RAATTY 3 cut(s) 635, 972, 1013
AcuI CTGAAG 1 cut(s) 116
AfiI CCNNNNNNNGG 3 cut(s) 41, 403, 952
AgsI TTSAA 2 cut(s) 122, 605
AjuI GAANNNNNNNTTGG 2 cut(s) 484, 516
AluBI AGCT 6 cut(s) 130, 139, 362, 507, 572, 593
AluI AGCT 6 cut(s) 130, 139, 362, 507, 572, 593
Alw26I GTCTC 1 cut(s) 209
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 5 cut(s) 101, 572, 824, 827, 992
ApoI RAATTY 3 cut(s) 635, 972, 1013
AseI ATTAAT 3 cut(s) 878, 999, 1038
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 4 cut(s) 434, 526, 704, 783
AsuII TTCGAA 1 cut(s) 970
AvaII GGWCC 1 cut(s) 371
BbvCI CCTCAGC 1 cut(s) 568
BbvI GCAGC 5 cut(s) 113, 559, 811, 814, 979
BccI CCATC 5 cut(s) 65, 113, 137, 377, 644
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 209
BfaI CTAG 5 cut(s) 50, 140, 161, 192, 767
BfmI CTRYAG 1 cut(s) 26
BisI GCNGC 8 cut(s) 102, 264, 573, 655, 825, 828, 990, 993
BlsI GCNGC 8 cut(s) 103, 265, 574, 656, 826, 829, 991, 994
Bme1390I CCNGG 1 cut(s) 187
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 187
BmsI GCATC 2 cut(s) 142, 526
BoxI GACNNNNGTC 1 cut(s) 756
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 470
Bpu10I CCTNAGC 1 cut(s) 568
Bpu14I TTCGAA 1 cut(s) 970
BpuEI CTTGAG 1 cut(s) 698
BpuMI CCSGG 1 cut(s) 187
BsaBI GATNNNNATC 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 619, 747
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 403, 952
Bse1I ACTGG 2 cut(s) 329, 790
Bse3DI GCAATG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 619, 747
BseGI GGATG 2 cut(s) 105, 157
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 3 cut(s) 41, 403, 952
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 3 cut(s) 329, 559, 582
BseNI ACTGG 2 cut(s) 329, 790
BseXI GCAGC 5 cut(s) 113, 559, 811, 814, 979
BseYI CCCAGC 2 cut(s) 97, 589
BsgI GTGCAG 2 cut(s) 419, 558
Bsh1236I CGCG 1 cut(s) 989
BshFI GGCC 1 cut(s) 90
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 3 cut(s) 41, 403, 952
BsmAI GTCTC 1 cut(s) 209
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 1 cut(s) 90
Bso31I GGTCTC 1 cut(s) 209
Bsp119I TTCGAA 1 cut(s) 970
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 5 cut(s) 250, 264, 654, 861, 989
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 3 cut(s) 328, 558, 581
BspFNI CGCG 1 cut(s) 989
BspT104I TTCGAA 1 cut(s) 970
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 558
BsrI ACTGG 2 cut(s) 329, 790
BssECI CCNNGG 2 cut(s) 619, 747
BssNAI GTATAC 1 cut(s) 20
BssT1I CCWWGG 2 cut(s) 619, 747
Bst1107I GTATAC 1 cut(s) 20
Bst4CI ACNGT 2 cut(s) 326, 346
BstBI TTCGAA 1 cut(s) 970
BstDEI CTNAG 3 cut(s) 315, 545, 568
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 2 cut(s) 105, 157
BstFNI CGCG 1 cut(s) 989
BstMAI GTCTC 1 cut(s) 209
BstMWI GCNNNNNNNGC 5 cut(s) 136, 161, 221, 569, 824
BstPAI GACNNNNGTC 1 cut(s) 756
BstSCI CCNGG 1 cut(s) 185
BstSFI CTRYAG 1 cut(s) 26
BstUI CGCG 1 cut(s) 989
BstV1I GCAGC 5 cut(s) 113, 559, 811, 814, 979
BstXI CCANNNNNNTGG 1 cut(s) 407
BstZ17I GTATAC 1 cut(s) 20
BsuRI GGCC 1 cut(s) 90
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 2 cut(s) 105, 157
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 3 cut(s) 295, 322, 336
Cfr13I GGNCC 1 cut(s) 371
CviAII CATG 5 cut(s) 217, 528, 748, 815, 821
DdeI CTNAG 3 cut(s) 315, 545, 568
Eco130I CCWWGG 2 cut(s) 619, 747
Eco147I AGGCCT 1 cut(s) 90
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 116
EcoRI GAATTC 1 cut(s) 972
EcoT14I CCWWGG 2 cut(s) 619, 747
EcoT22I ATGCAT 1 cut(s) 560
ErhI CCWWGG 2 cut(s) 619, 747
FaeI CATG 5 cut(s) 220, 531, 751, 818, 824
FaqI GGGAC 1 cut(s) 202
FatI CATG 5 cut(s) 216, 527, 747, 814, 820
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 8 cut(s) 102, 264, 573, 655, 825, 828, 990, 993
FokI GGATG 2 cut(s) 92, 164
Fsp4HI GCNGC 8 cut(s) 102, 264, 573, 655, 825, 828, 990, 993
FspBI CTAG 5 cut(s) 50, 140, 161, 192, 767
GluI GCNGC 8 cut(s) 102, 264, 573, 655, 825, 828, 990, 993
GsaI CCCAGC 2 cut(s) 101, 593
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 1 cut(s) 90
HapII CCGG 1 cut(s) 186
Hin1II CATG 5 cut(s) 220, 531, 751, 818, 824
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HindIII AAGCTT 1 cut(s) 360
HinfI GANTC 1 cut(s) 122
HpaI GTTAAC 1 cut(s) 855
HpaII CCGG 1 cut(s) 186
HphI GGTGA 4 cut(s) 434, 526, 704, 783
Hpy166II GTNNAC 3 cut(s) 20, 855, 866
Hpy188I TCNGA 8 cut(s) 127, 135, 318, 535, 548, 727, 907, 955
Hpy8I GTNNAC 3 cut(s) 20, 855, 866
HpyCH4III ACNGT 2 cut(s) 326, 346
HpyCH4IV ACGT 1 cut(s) 627
HpyCH4V TGCA 8 cut(s) 155, 224, 436, 558, 563, 575, 665, 1027
HpyF10VI GCNNNNNNNGC 5 cut(s) 136, 161, 221, 569, 824
HpyF3I CTNAG 3 cut(s) 315, 545, 568
HpySE526I ACGT 1 cut(s) 627
Hsp92II CATG 5 cut(s) 220, 531, 751, 818, 824
KspAI GTTAAC 1 cut(s) 855
LmnI GCTCC 1 cut(s) 536
Lsp1109I GCAGC 5 cut(s) 113, 559, 811, 814, 979
LweI GCATC 2 cut(s) 142, 526
MaeI CTAG 5 cut(s) 50, 140, 161, 192, 767
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 4 cut(s) 52, 208, 422, 697
MboII GAAGA 2 cut(s) 301, 959
MluCI AATT 6 cut(s) 416, 635, 879, 972, 1013, 1035
MmeI TCCRAC 1 cut(s) 825
MnlI CCTC 7 cut(s) 33, 80, 312, 324, 505, 577, 949
Mph1103I ATGCAT 1 cut(s) 560
MseI TTAA 9 cut(s) 276, 630, 639, 741, 854, 878, 921, 999, 1038
MslI CAYNNNNRTG 1 cut(s) 819
MspA1I CMGCKG 2 cut(s) 572, 861
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 1 cut(s) 187
MvnI CGCG 1 cut(s) 989
MwoI GCNNNNNNNGC 5 cut(s) 136, 161, 221, 569, 824
NciI CCSGG 1 cut(s) 187
NcoI CCATGG 1 cut(s) 747
NlaIII CATG 5 cut(s) 220, 531, 751, 818, 824
NmuCI GTSAC 2 cut(s) 422, 697
NsiI ATGCAT 1 cut(s) 560
NspV TTCGAA 1 cut(s) 970
PceI AGGCCT 1 cut(s) 90
PfeI GAWTC 1 cut(s) 122
PflMI CCANNNNNTGG 1 cut(s) 403
PkrI GCNGC 8 cut(s) 103, 265, 574, 656, 826, 829, 991, 994
PshAI GACNNNNGTC 1 cut(s) 756
PshBI ATTAAT 3 cut(s) 878, 999, 1038
Psp1406I AACGTT 1 cut(s) 627
PspFI CCCAGC 2 cut(s) 97, 589
PspPI GGNCC 1 cut(s) 371
PvuII CAGCTG 1 cut(s) 572
RseI CAYNNNNRTG 1 cut(s) 819
SaqAI TTAA 9 cut(s) 276, 630, 639, 741, 854, 878, 921, 999, 1038
SatI GCNGC 8 cut(s) 102, 264, 573, 655, 825, 828, 990, 993
Sau96I GGNCC 1 cut(s) 371
ScrFI CCNGG 1 cut(s) 187
SfaNI GCATC 2 cut(s) 142, 526
SfcI CTRYAG 1 cut(s) 26
SfuI TTCGAA 1 cut(s) 970
SinI GGWCC 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse9I AATT 6 cut(s) 416, 635, 879, 972, 1013, 1035
SseBI AGGCCT 1 cut(s) 90
SsiI CCGC 5 cut(s) 250, 264, 654, 861, 989
SspI AATATT 1 cut(s) 1042
SspMI CTAG 5 cut(s) 50, 140, 161, 192, 767
StuI AGGCCT 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 2 cut(s) 619, 747
TaaI ACNGT 2 cut(s) 326, 346
TaiI ACGT 1 cut(s) 630
TaqI TCGA 3 cut(s) 760, 915, 970
TasI AATT 6 cut(s) 416, 635, 879, 972, 1013, 1035
TauI GCSGC 3 cut(s) 266, 657, 992
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 9 cut(s) 276, 630, 639, 741, 854, 878, 921, 999, 1038
Tru9I TTAA 9 cut(s) 276, 630, 639, 741, 854, 878, 921, 999, 1038
TscAI CASTG 3 cut(s) 302, 329, 336
TseFI GTSAC 2 cut(s) 422, 697
TseI GCWGC 5 cut(s) 101, 572, 824, 827, 992
Tsp45I GTSAC 2 cut(s) 422, 697
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 3 cut(s) 302, 329, 336
Van91I CCANNNNNTGG 1 cut(s) 403
VpaK11BI GGWCC 1 cut(s) 371
VspI ATTAAT 3 cut(s) 878, 999, 1038
XapI RAATTY 3 cut(s) 635, 972, 1013
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 5 cut(s) 50, 140, 161, 192, 767
Zsp2I ATGCAT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.