MD15G1086000.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000265
Physical Location & Seq
Reverse (-)
5963082 .. 5965502
2421 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1086000.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGTTGGAAATGAACTATCTAAAGGGAGAGGTTCATTCCTGGAAAATCATGGTATATACGTATTCGCGCAAGCAAGCTACCAAGCTAAAGTCTATAAATACCAACTACTCTCAAGGAGAAATCACAATCAAATTAAGAACTCTAGCTCATCAATTGTTCAGGCTCTATCTCCCTCTCCCTTTCTCAAACCTTCTAATGGAAATAACCAGTAGAAAGCTCATCACAGTTCTCAGCATCGACGGCGGCGGGATAAGAGGCCTTATTCCAGCCACCATCCTCGGTTTTCTTGAATCTGAACTTCAGAAGCTGGACGGTGAGGATGCAAGAATTGCCGACTACTTCGATGTGATCGCAGGAACAAGCACTGGTGGTCTTGTGACAGCCATGCTCACAACCCCAAACGAGAATGACCGCCCACTGTTTGCTGCCGAAGATATTAAGGACTTTTACCTAAGTGAATGCCCTAAAATCTTCCCCCAAAAGATTTGGCTTCCGTATACAAGAAAGATTATGAAAGCTCTAACAGGACCAAAATACGACGGTAAGTATCTACATGGCTTGATCAGGCAAAAATTAGGCAACAACAAGTTGCACAACACATTGACTAATGTTGTCATTCCCACATTCGACATTCGGAATCTCCAGCCAGTTGTCTTCTCTAGCCTTAGGGTGGAAAAGAAGCCGTGCACCGATGCCTTACTCTCCGACATATGCATTGCAACCTCAGCAGCACCAACTTACCTTCCAGCTCATTATTTTGAAACCAAGTATGCTGCAGGAACAGTTAGAGAATATAACCTTATAGATGGCGGCGTGGCTGCAAATAATCCGACATTTGTTGCTATTTGTGAAGTGATGAACGAAATAGAGAAGGAGAATTCAGATATCGTTCCTGTAAAACTCATGGACTATGGACGATTTCTAGTAATATCATTAGGAACTGGCTCATCAAAAGCTGAACGGAAATACCATGCACCTGCTGCAGCCAAATGGGGCCTCTTGAATTGGTTAACCAGCGGTGGTTCCACCCCGATCATTGACGTTTTCAGTCAAGCGAGCGCCGATATGGTTGATCTTCACCTTTCTGGCGTTTTTCAAGCCCTTAATTCTGAAGAAAACTATCTTCGAATTCAGGACGATACCCTTCACGGGACAGTATCTTCTGTAGATGTGGCAACAAAGAAAAATTTGGCCAATCTTGTGAAAGTTGGTGAAGGGCTATTGAAACAACCAGTTTCCAGGGTTAATTTGGACAGTGGCAGGTTTGAGGCTTCTAATCATGAGACTAATGCAGAGGCTCTTACAAGGTTTGCTAAACTACTCTCCGAAGAGAAGCTGCTTCGCCAAGCAAGCAGGTCAACTAATGAGCCTGCTGGAAATACCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

463

Amino Acids

51.23

Weight (kDa)

8.13

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 77 - 282 3.6e-27 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 985
Acc36I ACCTGC 3 cut(s) 985, 1251, 1344
AccI GTMKAC 1 cut(s) 497
AccII CGCG 1 cut(s) 67
AciI CCGC 5 cut(s) 243, 246, 412, 810, 1017
AcoI YGGCCR 1 cut(s) 1191
AcsI RAATTY 3 cut(s) 877, 1128, 1186
AcuI CTGAAG 2 cut(s) 284, 1131
AfiI CCNNNNNNNGG 3 cut(s) 196, 670, 1149
AgsI TTSAA 5 cut(s) 290, 761, 1003, 1097, 1225
AjnI CCWGG 2 cut(s) 38, 1238
AluBI AGCT 9 cut(s) 77, 85, 146, 217, 307, 518, 749, 956, 1336
AluI AGCT 9 cut(s) 77, 85, 146, 217, 307, 518, 749, 956, 1336
Alw21I GWGCWC 1 cut(s) 689
Alw26I GTCTC 1 cut(s) 1277
Alw44I GTGCAC 1 cut(s) 685
AlwNI CAGNNNCTG 1 cut(s) 307
AoxI GGCC 3 cut(s) 256, 994, 1191
ApaLI GTGCAC 1 cut(s) 685
ApeKI GCWGC 7 cut(s) 425, 728, 773, 818, 980, 983, 1336
ApoI RAATTY 3 cut(s) 877, 1128, 1186
AspLEI GCGC 2 cut(s) 69, 1061
AspS9I GGNCC 2 cut(s) 527, 994
AsuHPI GGTGA 3 cut(s) 326, 1070, 1223
AsuII TTCGAA 1 cut(s) 1126
AvaII GGWCC 1 cut(s) 527
AxyI CCTNAGG 1 cut(s) 665
BaeGI GKGCMC 1 cut(s) 689
BalI TGGCCA 1 cut(s) 1193
BbsI GAAGAC 1 cut(s) 646
Bbv12I GWGCWC 1 cut(s) 689
BbvCI CCTCAGC 1 cut(s) 724
BbvI GCAGC 7 cut(s) 412, 740, 760, 805, 967, 995, 1323
BccI CCATC 2 cut(s) 281, 800
BceAI ACGGC 2 cut(s) 256, 667
BciT130I CCWGG 2 cut(s) 40, 1240
BclI TGATCA 1 cut(s) 561
BcoDI GTCTC 1 cut(s) 1277
BfaI CTAG 3 cut(s) 143, 660, 923
BfmI CTRYAG 3 cut(s) 774, 981, 1164
BfoI RGCGCY 1 cut(s) 1062
BfuAI ACCTGC 3 cut(s) 985, 1251, 1344
BisI GCNGC 9 cut(s) 244, 426, 729, 774, 811, 819, 981, 984, 1337
BlsI GCNGC 9 cut(s) 245, 427, 730, 775, 812, 820, 982, 985, 1338
Bme1390I CCNGG 2 cut(s) 40, 1240
Bme18I GGWCC 1 cut(s) 527
BmgT120I GGNCC 2 cut(s) 527, 994
BmiI GGNNCC 2 cut(s) 995, 1024
BmrFI CCNGG 2 cut(s) 40, 1240
BmsI GCATC 3 cut(s) 243, 310, 682
BpiI GAAGAC 1 cut(s) 646
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 724
Bpu14I TTCGAA 1 cut(s) 1126
BpuEI CTTGAG 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 277, 1239
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 670, 1149
Bse1I ACTGG 5 cut(s) 207, 370, 647, 946, 1232
Bse21I CCTNAGG 1 cut(s) 665
Bse3DI GCAATG 1 cut(s) 714
BseBI CCWGG 2 cut(s) 40, 1240
BseDI CCNNGG 2 cut(s) 277, 1239
BseGI GGATG 2 cut(s) 273, 325
BseLI CCNNNNNNNGG 3 cut(s) 196, 670, 1149
BseMI GCAATG 1 cut(s) 714
BseMII CTCAG 2 cut(s) 244, 738
BseNI ACTGG 5 cut(s) 207, 370, 647, 946, 1232
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 7 cut(s) 412, 740, 760, 805, 967, 995, 1323
Bsh1236I CGCG 1 cut(s) 67
BshFI GGCC 3 cut(s) 258, 996, 1193
BsiHKAI GWGCWC 1 cut(s) 689
BslFI GGGAC 1 cut(s) 1165
BslI CCNNNNNNNGG 3 cut(s) 196, 670, 1149
BsmAI GTCTC 1 cut(s) 1277
BsmFI GGGAC 1 cut(s) 1165
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 3 cut(s) 258, 996, 1193
Bsp119I TTCGAA 1 cut(s) 1126
Bsp1286I GDGCHC 1 cut(s) 689
Bsp143I GATC 4 cut(s) 348, 561, 1032, 1072
BspACI CCGC 5 cut(s) 243, 246, 412, 810, 1017
BspANI GGCC 3 cut(s) 258, 996, 1193
BspCNI CTCAG 2 cut(s) 243, 737
BspFNI CGCG 1 cut(s) 67
BspHI TCATGA 1 cut(s) 1279
BspLI GGNNCC 2 cut(s) 995, 1024
BspMAI CTGCAG 2 cut(s) 778, 985
BspMI ACCTGC 3 cut(s) 985, 1251, 1344
BspT104I TTCGAA 1 cut(s) 1126
BsrDI GCAATG 1 cut(s) 714
BsrI ACTGG 5 cut(s) 207, 370, 647, 946, 1232
BssECI CCNNGG 2 cut(s) 277, 1239
BssMI GATC 4 cut(s) 348, 561, 1032, 1072
BssNAI GTATAC 1 cut(s) 498
Bst1107I GTATAC 1 cut(s) 498
Bst2UI CCWGG 2 cut(s) 40, 1240
Bst4CI ACNGT 7 cut(s) 226, 314, 420, 542, 784, 1156, 1256
Bst6I CTCTTC 1 cut(s) 1323
BstAPI GCANNNNNTGC 2 cut(s) 329, 980
BstBAI YACGTR 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 1126
BstC8I GCNNGC 5 cut(s) 71, 75, 1057, 1351, 1371
BstDEI CTNAG 4 cut(s) 230, 452, 665, 724
BstF5I GGATG 2 cut(s) 273, 325
BstFNI CGCG 1 cut(s) 67
BstH2I RGCGCY 1 cut(s) 1062
BstHHI GCGC 2 cut(s) 69, 1061
BstKTI GATC 4 cut(s) 351, 564, 1035, 1075
BstMAI GTCTC 1 cut(s) 1277
BstMBI GATC 4 cut(s) 348, 561, 1032, 1072
BstMWI GCNNNNNNNGC 5 cut(s) 240, 329, 725, 980, 1350
BstNI CCWGG 2 cut(s) 40, 1240
BstSCI CCNGG 2 cut(s) 38, 1238
BstSFI CTRYAG 3 cut(s) 774, 981, 1164
BstSLI GKGCMC 1 cut(s) 689
BstSNI TACGTA 1 cut(s) 60
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 7 cut(s) 412, 740, 760, 805, 967, 995, 1323
BstV2I GAAGAC 1 cut(s) 646
BstZ17I GTATAC 1 cut(s) 498
Bsu36I CCTNAGG 1 cut(s) 665
BsuRI GGCC 3 cut(s) 258, 996, 1193
BtsCI GGATG 2 cut(s) 273, 325
BtsIMutI CAGTG 3 cut(s) 363, 416, 1261
BveI ACCTGC 3 cut(s) 985, 1251, 1344
Cac8I GCNNGC 5 cut(s) 71, 75, 1057, 1351, 1371
CaiI CAGNNNCTG 1 cut(s) 307
CciI TCATGA 1 cut(s) 1279
CfoI GCGC 2 cut(s) 69, 1061
Cfr13I GGNCC 2 cut(s) 527, 994
CviAII CATG 6 cut(s) 49, 385, 554, 904, 971, 1280
DdeI CTNAG 4 cut(s) 230, 452, 665, 724
DpnI GATC 4 cut(s) 350, 563, 1034, 1074
DpnII GATC 4 cut(s) 348, 561, 1032, 1072
EaeI YGGCCR 1 cut(s) 1191
Eam1104I CTCTTC 1 cut(s) 1323
EarI CTCTTC 1 cut(s) 1323
Eco105I TACGTA 1 cut(s) 60
Eco147I AGGCCT 1 cut(s) 258
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 2 cut(s) 284, 1131
Eco81I CCTNAGG 1 cut(s) 665
EcoO109I RGGNCCY 1 cut(s) 994
EcoRI GAATTC 2 cut(s) 877, 1128
EcoRII CCWGG 2 cut(s) 38, 1238
EcoRV GATATC 1 cut(s) 886
EcoT22I ATGCAT 1 cut(s) 716
FaeI CATG 6 cut(s) 52, 388, 557, 907, 974, 1283
FaqI GGGAC 1 cut(s) 1165
FatI CATG 6 cut(s) 48, 384, 553, 903, 970, 1279
FauI CCCGC 1 cut(s) 239
FauNDI CATATG 1 cut(s) 710
FbaI TGATCA 1 cut(s) 561
FblI GTMKAC 1 cut(s) 497
Fnu4HI GCNGC 9 cut(s) 244, 426, 729, 774, 811, 819, 981, 984, 1337
FokI GGATG 2 cut(s) 260, 332
Fsp4HI GCNGC 9 cut(s) 244, 426, 729, 774, 811, 819, 981, 984, 1337
FspBI CTAG 3 cut(s) 143, 660, 923
GlaI GCGC 2 cut(s) 68, 1060
GluI GCNGC 9 cut(s) 244, 426, 729, 774, 811, 819, 981, 984, 1337
GsuI CTGGAG 1 cut(s) 626
HaeII RGCGCY 1 cut(s) 1062
HaeIII GGCC 3 cut(s) 258, 996, 1193
HhaI GCGC 2 cut(s) 69, 1061
Hin1II CATG 6 cut(s) 52, 388, 557, 907, 974, 1283
Hin6I GCGC 2 cut(s) 67, 1059
HinP1I GCGC 2 cut(s) 67, 1059
HincII GTYRAC 2 cut(s) 1011, 1359
HindII GTYRAC 2 cut(s) 1011, 1359
HinfI GANTC 2 cut(s) 290, 637
HpaI GTTAAC 1 cut(s) 1011
HphI GGTGA 3 cut(s) 326, 1070, 1223
Hpy166II GTNNAC 4 cut(s) 498, 687, 1011, 1359
Hpy188I TCNGA 8 cut(s) 295, 303, 636, 706, 831, 883, 1111, 1327
Hpy188III TCNNGA 4 cut(s) 287, 1000, 1133, 1280
Hpy8I GTNNAC 4 cut(s) 498, 687, 1011, 1359
Hpy99I CGWCG 2 cut(s) 242, 542
HpyAV CCTTC 5 cut(s) 200, 752, 865, 1154, 1208
HpyCH4III ACNGT 7 cut(s) 226, 314, 420, 542, 784, 1156, 1256
HpyCH4IV ACGT 2 cut(s) 59, 1041
HpyF10VI GCNNNNNNNGC 5 cut(s) 240, 329, 725, 980, 1350
HpyF3I CTNAG 4 cut(s) 230, 452, 665, 724
HpySE526I ACGT 2 cut(s) 59, 1041
Hsp92II CATG 6 cut(s) 52, 388, 557, 907, 974, 1283
HspAI GCGC 2 cut(s) 67, 1059
Ksp22I TGATCA 1 cut(s) 561
KspAI GTTAAC 1 cut(s) 1011
Kzo9I GATC 4 cut(s) 348, 561, 1032, 1072
Lsp1109I GCAGC 7 cut(s) 412, 740, 760, 805, 967, 995, 1323
LweI GCATC 3 cut(s) 243, 310, 682
MaeI CTAG 3 cut(s) 143, 660, 923
MaeII ACGT 2 cut(s) 59, 1041
MaeIII GTNAC 1 cut(s) 376
MalI GATC 4 cut(s) 350, 563, 1034, 1074
MboI GATC 4 cut(s) 348, 561, 1032, 1072
MboII GAAGA 8 cut(s) 443, 463, 646, 1067, 1115, 1124, 1152, 1340
MfeI CAATTG 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 689
MlsI TGGCCA 1 cut(s) 1193
MluNI TGGCCA 1 cut(s) 1193
MmeI TCCRAC 2 cut(s) 729, 854
MnlI CCTC 9 cut(s) 22, 183, 248, 287, 310, 733, 1007, 1261, 1288
Mox20I TGGCCA 1 cut(s) 1193
Mph1103I ATGCAT 1 cut(s) 716
MscI TGGCCA 1 cut(s) 1193
MseI TTAA 5 cut(s) 134, 438, 1010, 1104, 1245
Msp20I TGGCCA 1 cut(s) 1193
MspA1I CMGCKG 1 cut(s) 1017
MspR9I CCNGG 2 cut(s) 40, 1240
MunI CAATTG 1 cut(s) 152
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 2 cut(s) 40, 1240
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 5 cut(s) 240, 329, 725, 980, 1350
NdeI CATATG 1 cut(s) 710
NdeII GATC 4 cut(s) 348, 561, 1032, 1072
NlaIII CATG 6 cut(s) 52, 388, 557, 907, 974, 1283
NlaIV GGNNCC 2 cut(s) 995, 1024
NmuCI GTSAC 1 cut(s) 376
NsiI ATGCAT 1 cut(s) 716
NspV TTCGAA 1 cut(s) 1126
PagI TCATGA 1 cut(s) 1279
PaqCI CACCTGC 1 cut(s) 985
PceI AGGCCT 1 cut(s) 258
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 2 cut(s) 290, 637
PfoI TCCNGGA 1 cut(s) 38
PkrI GCNGC 9 cut(s) 245, 427, 730, 775, 812, 820, 982, 985, 1338
Ppu21I YACGTR 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 38, 1238
PspGI CCWGG 2 cut(s) 38, 1238
PspN4I GGNNCC 2 cut(s) 995, 1024
PspPI GGNCC 2 cut(s) 527, 994
PstI CTGCAG 2 cut(s) 778, 985
PstNI CAGNNNCTG 1 cut(s) 307
SaqAI TTAA 5 cut(s) 134, 438, 1010, 1104, 1245
SatI GCNGC 9 cut(s) 244, 426, 729, 774, 811, 819, 981, 984, 1337
Sau3AI GATC 4 cut(s) 348, 561, 1032, 1072
Sau96I GGNCC 2 cut(s) 527, 994
ScrFI CCNGG 2 cut(s) 40, 1240
SduI GDGCHC 1 cut(s) 689
SfaNI GCATC 3 cut(s) 243, 310, 682
SfcI CTRYAG 3 cut(s) 774, 981, 1164
SfuI TTCGAA 1 cut(s) 1126
SinI GGWCC 1 cut(s) 527
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
SnaBI TACGTA 1 cut(s) 60
SseBI AGGCCT 1 cut(s) 258
SsiI CCGC 5 cut(s) 243, 246, 412, 810, 1017
SspMI CTAG 3 cut(s) 143, 660, 923
StuI AGGCCT 1 cut(s) 258
StyD4I CCNGG 2 cut(s) 38, 1238
TaaI ACNGT 7 cut(s) 226, 314, 420, 542, 784, 1156, 1256
TaiI ACGT 2 cut(s) 62, 1044
TaqI TCGA 4 cut(s) 237, 342, 627, 1126
TauI GCSGC 2 cut(s) 246, 813
TfiI GAWTC 2 cut(s) 290, 637
Tru1I TTAA 5 cut(s) 134, 438, 1010, 1104, 1245
Tru9I TTAA 5 cut(s) 134, 438, 1010, 1104, 1245
TscAI CASTG 3 cut(s) 370, 423, 1261
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 7 cut(s) 425, 728, 773, 818, 980, 983, 1336
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 4 cut(s) 23, 26, 527, 872
TspGWI ACGGA 2 cut(s) 483, 976
TspRI CASTG 3 cut(s) 370, 423, 1261
VneI GTGCAC 1 cut(s) 685
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 3 cut(s) 877, 1128, 1186
XcmI CCANNNNNNNNNTGG 1 cut(s) 1246
XmiI GTMKAC 1 cut(s) 497
XspI CTAG 3 cut(s) 143, 660, 923
Zsp2I ATGCAT 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.