MD15G1086500.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000265
Physical Location & Seq
Reverse (-)
5999969 .. 6001975
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1086500.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGAAAGAAGCGGTAGCGTAGGGCGCCCCCCAGTCTTAGGAAAGATGGTCAGAGTTCTCAGTATCGATGGGGGCGGAATAAGAGGCCTCATTCCGGCAACCATTCTTGCTTTCCTTGAATCTGAACTTCAGAAGCTGGATGGTGAAGATGCAAGAATCGCCGACTATTTTGATGTGATTGCAGGAACAAGCACAGGTGGTCTTGTGACTGCTATGCTCGCAGCCCCAAACGAGAATAACCGCCCACTATTCGCCGCCAAAGATATAATTGACTTCTACTTTCATCACTGCCCTAAAATCTTCCCCCAGAACACTTGCCCAATTTTTCGTTATACGATAAAGATTATTAAAGCTCTAGCAGGACCAAAATACAATGGAAAATATCTGCATAGCTTGATCAGAGAAAAAATGGGCAACAAAAAACTACATGAAACATTGACTAATGTTGTCATTCCCACGTTTGACATCAAGAATCTCCAGCCAACTGTCTTCTCCAACCATAGGGTGAAAAGGAAGCCATCCTACAATGCCTTACTCTCTGACATATGGATTGCAACCTCAGCAGCACCAACTTATCTTCCAGCTCATTATTTTAAAACCAAGGACGCTGCAGGCAGAGATAAAGAATTCAACCTTATAGATGGCGGCGTGGCTGCAAATAATCCAACTTTACTTGCTGTTTGTGAAATGACGAACGAAATAAAGAAGGAGAATCCAGTCCTCTTTCCCGTGAAACCTGTGGACTATGGGAGATTTCTAGTGATATCATTAGGAACTGGCTCATCAAAAGCTGAACTGAAATACCATGCACGTTCTGCAGCAAAATGGGGCCTGTTGAATTGGTTAACCAGCGGTGGCTCCACCCCAATCATTGATGTTTTCAGTCAAGCAAGCGCTGACATGGTTGATCTTCACCTTTCTGGAGTTTTCCAAGCCCTTCATTCTGAAAAAAACTATCTTAGAATTCAGGATGATACCCTTCATGGGACCATATCTTCTGTGGATATAGCCACGAAGAAGAATTTGGCCAATCTTGTGAAAGTTGGTGAAGGGCTATTGAAACAACAAGTTTCTCGGGTTAATTTGGACAGCGGTAAATTTGAGGCTTCTAATCATGAGACAAATGCAGAGGCTCTTATAAGGTTTGCTAAACTACTCTCGAAAGAGAAGAGGAGTCGCCAAGCAGGGTCAACCAACGGGAATGCTGAAAGTACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

407

Amino Acids

44.53

Weight (kDa)

9.34

Isoelectric Point (pI)

27.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 20 - 226 5.7e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1139
AccB1I GGYRCC 1 cut(s) 24
AciI CCGC 7 cut(s) 12, 75, 241, 255, 645, 852, 1092
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 4 cut(s) 626, 963, 1021, 1097
AcuI CTGAAG 1 cut(s) 113
AcyI GRCGYC 1 cut(s) 25
AfaI GTAC 1 cut(s) 1213
AfeI AGCGCT 1 cut(s) 895
AfiI CCNNNNNNNGG 4 cut(s) 38, 94, 501, 984
AgsI TTSAA 4 cut(s) 119, 631, 838, 1060
AjuI GAANNNNNNNTTGG 2 cut(s) 1007, 1039
AluBI AGCT 5 cut(s) 136, 353, 393, 584, 791
AluI AGCT 5 cut(s) 136, 353, 393, 584, 791
Alw26I GTCTC 1 cut(s) 1112
AlwNI CAGNNNCTG 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 1074
Aor51HI AGCGCT 1 cut(s) 895
AoxI GGCC 3 cut(s) 85, 829, 1026
ApeKI GCWGC 5 cut(s) 221, 563, 608, 653, 818
ApoI RAATTY 4 cut(s) 626, 963, 1021, 1097
AspLEI GCGC 2 cut(s) 27, 896
AspS9I GGNCC 3 cut(s) 362, 829, 987
AsuHPI GGTGA 4 cut(s) 155, 517, 905, 1058
AvaI CYCGRG 1 cut(s) 1074
AvaII GGWCC 2 cut(s) 362, 987
BalI TGGCCA 1 cut(s) 1028
BanI GGYRCC 1 cut(s) 24
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BbsI GAAGAC 1 cut(s) 481
BbvCI CCTCAGC 1 cut(s) 559
BbvI GCAGC 5 cut(s) 233, 575, 595, 640, 830
BccI CCATC 5 cut(s) 40, 62, 134, 526, 635
BclI TGATCA 1 cut(s) 396
BcoDI GTCTC 1 cut(s) 1112
BfaI CTAG 2 cut(s) 356, 758
BfmI CTRYAG 2 cut(s) 609, 816
BfoI RGCGCY 2 cut(s) 28, 897
BisI GCNGC 7 cut(s) 222, 255, 564, 609, 646, 654, 819
BlsI GCNGC 7 cut(s) 223, 256, 565, 610, 647, 655, 820
BmcAI AGTACT 1 cut(s) 1213
Bme18I GGWCC 2 cut(s) 362, 987
BmeT110I CYCGRG 1 cut(s) 1074
BmgT120I GGNCC 3 cut(s) 362, 829, 987
BmiI GGNNCC 4 cut(s) 26, 830, 859, 988
BmrI ACTGGG 1 cut(s) 26
BmsI GCATC 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 481
BpmI CTGGAG 2 cut(s) 461, 942
Bpu10I CCTNAGC 1 cut(s) 559
Bsa29I ATCGAT 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 25
BsaJI CCNNGG 1 cut(s) 600
Bsc4I CCNNNNNNNGG 4 cut(s) 38, 94, 501, 984
Bse1I ACTGG 3 cut(s) 32, 716, 781
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 600
BseGI GGATG 3 cut(s) 145, 518, 976
BseLI CCNNNNNNNGG 4 cut(s) 38, 94, 501, 984
BseMII CTCAG 2 cut(s) 73, 573
BseNI ACTGG 3 cut(s) 32, 716, 781
BseRI GAGGAG 1 cut(s) 1186
BseXI GCAGC 5 cut(s) 233, 575, 595, 640, 830
BshFI GGCC 3 cut(s) 87, 831, 1028
BshNI GGYRCC 1 cut(s) 24
BshVI ATCGAT 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 1074
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 1 cut(s) 1000
BslI CCNNNNNNNGG 4 cut(s) 38, 94, 501, 984
BsmAI GTCTC 1 cut(s) 1112
BsmFI GGGAC 1 cut(s) 1000
BsmI GAATGC 1 cut(s) 1207
BsnI GGCC 3 cut(s) 87, 831, 1028
BsoBI CYCGRG 1 cut(s) 1074
Bsp143I GATC 2 cut(s) 396, 907
BspACI CCGC 7 cut(s) 12, 75, 241, 255, 645, 852, 1092
BspANI GGCC 3 cut(s) 87, 831, 1028
BspCNI CTCAG 2 cut(s) 72, 572
BspDI ATCGAT 1 cut(s) 66
BspHI TCATGA 1 cut(s) 1114
BspLI GGNNCC 4 cut(s) 26, 830, 859, 988
BspMAI CTGCAG 2 cut(s) 613, 820
BspT107I GGYRCC 1 cut(s) 24
BsrI ACTGG 3 cut(s) 32, 716, 781
BssECI CCNNGG 1 cut(s) 600
BssMI GATC 2 cut(s) 396, 907
BssNI GRCGYC 1 cut(s) 25
BssT1I CCWWGG 1 cut(s) 600
Bst4CI ACNGT 1 cut(s) 487
Bst6I CTCTTC 1 cut(s) 1163
BstACI GRCGYC 1 cut(s) 25
BstAPI GCANNNNNTGC 1 cut(s) 815
BstC8I GCNNGC 3 cut(s) 219, 613, 892
BstDEI CTNAG 4 cut(s) 37, 59, 559, 959
BstF5I GGATG 3 cut(s) 145, 518, 976
BstH2I RGCGCY 2 cut(s) 28, 897
BstHHI GCGC 2 cut(s) 27, 896
BstKTI GATC 2 cut(s) 399, 910
BstMAI GTCTC 1 cut(s) 1112
BstMBI GATC 2 cut(s) 396, 907
BstMWI GCNNNNNNNGC 5 cut(s) 24, 158, 218, 560, 815
BstSFI CTRYAG 2 cut(s) 609, 816
BstV1I GCAGC 5 cut(s) 233, 575, 595, 640, 830
BstV2I GAAGAC 1 cut(s) 481
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 3 cut(s) 87, 831, 1028
BsuTUI ATCGAT 1 cut(s) 66
BtsCI GGATG 3 cut(s) 145, 518, 976
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 219, 613, 892
CaiI CAGNNNCTG 1 cut(s) 136
CciI TCATGA 1 cut(s) 1114
CfoI GCGC 2 cut(s) 27, 896
Cfr13I GGNCC 3 cut(s) 362, 829, 987
ClaI ATCGAT 1 cut(s) 66
CseI GACGC 1 cut(s) 614
Csp6I GTAC 1 cut(s) 1212
CviAII CATG 5 cut(s) 428, 806, 901, 983, 1115
CviQI GTAC 1 cut(s) 1212
DdeI CTNAG 4 cut(s) 37, 59, 559, 959
DinI GGCGCC 1 cut(s) 26
DpnI GATC 2 cut(s) 398, 909
DpnII GATC 2 cut(s) 396, 907
DraI TTTAAA 1 cut(s) 595
EaeI YGGCCR 1 cut(s) 1026
Eam1104I CTCTTC 1 cut(s) 1163
EarI CTCTTC 1 cut(s) 1163
EciI GGCGGA 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 600
Eco147I AGGCCT 1 cut(s) 87
Eco32I GATATC 1 cut(s) 765
Eco47I GGWCC 2 cut(s) 362, 987
Eco47III AGCGCT 1 cut(s) 895
Eco57I CTGAAG 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 1074
EcoO109I RGGNCCY 1 cut(s) 829
EcoRI GAATTC 2 cut(s) 626, 963
EcoRV GATATC 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 600
EgeI GGCGCC 1 cut(s) 26
EheI GGCGCC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 5 cut(s) 431, 809, 904, 986, 1118
FaqI GGGAC 1 cut(s) 1000
FatI CATG 5 cut(s) 427, 805, 900, 982, 1114
FauNDI CATATG 1 cut(s) 545
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 7 cut(s) 222, 255, 564, 609, 646, 654, 819
FokI GGATG 3 cut(s) 152, 505, 983
Fsp4HI GCNGC 7 cut(s) 222, 255, 564, 609, 646, 654, 819
FspBI CTAG 2 cut(s) 356, 758
GlaI GCGC 2 cut(s) 26, 895
GluI GCNGC 7 cut(s) 222, 255, 564, 609, 646, 654, 819
GsuI CTGGAG 2 cut(s) 461, 942
HaeII RGCGCY 2 cut(s) 28, 897
HaeIII GGCC 3 cut(s) 87, 831, 1028
HapII CCGG 1 cut(s) 95
HgaI GACGC 1 cut(s) 614
HhaI GCGC 2 cut(s) 27, 896
Hin1I GRCGYC 1 cut(s) 25
Hin1II CATG 5 cut(s) 431, 809, 904, 986, 1118
Hin6I GCGC 2 cut(s) 25, 894
HinP1I GCGC 2 cut(s) 25, 894
HincII GTYRAC 2 cut(s) 846, 1191
HindII GTYRAC 2 cut(s) 846, 1191
HinfI GANTC 5 cut(s) 119, 156, 472, 712, 1174
HpaI GTTAAC 1 cut(s) 846
HpaII CCGG 1 cut(s) 95
HphI GGTGA 4 cut(s) 155, 517, 905, 1058
Hpy166II GTNNAC 3 cut(s) 742, 846, 1191
Hpy188I TCNGA 6 cut(s) 53, 124, 132, 401, 541, 946
Hpy188III TCNNGA 5 cut(s) 469, 921, 968, 1115, 1159
Hpy8I GTNNAC 3 cut(s) 742, 846, 1191
HpyAV CCTTC 4 cut(s) 700, 947, 989, 1043
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4IV ACGT 2 cut(s) 458, 811
HpyCH4V TGCA 9 cut(s) 152, 182, 388, 554, 611, 656, 809, 818, 1127
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 158, 218, 560, 815
HpyF3I CTNAG 4 cut(s) 37, 59, 559, 959
HpySE526I ACGT 2 cut(s) 458, 811
Hsp92I GRCGYC 1 cut(s) 25
Hsp92II CATG 5 cut(s) 431, 809, 904, 986, 1118
HspAI GCGC 2 cut(s) 25, 894
KasI GGCGCC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 396
KspAI GTTAAC 1 cut(s) 846
Kzo9I GATC 2 cut(s) 396, 907
LmnI GCTCC 1 cut(s) 863
Lsp1109I GCAGC 5 cut(s) 233, 575, 595, 640, 830
LweI GCATC 1 cut(s) 139
MaeI CTAG 2 cut(s) 356, 758
MaeII ACGT 2 cut(s) 458, 811
MaeIII GTNAC 1 cut(s) 205
MalI GATC 2 cut(s) 398, 909
MboI GATC 2 cut(s) 396, 907
MboII GAAGA 9 cut(s) 158, 292, 481, 569, 902, 987, 1027, 1030, 1180
MlsI TGGCCA 1 cut(s) 1028
MluCI AATT 8 cut(s) 267, 321, 626, 838, 963, 1021, 1081, 1097
MluNI TGGCCA 1 cut(s) 1028
Mly113I GGCGCC 1 cut(s) 25
MlyI GAGTC 1 cut(s) 1183
MmeI TCCRAC 2 cut(s) 519, 689
MnlI CCTC 7 cut(s) 77, 98, 568, 731, 1096, 1123, 1164
Mox20I TGGCCA 1 cut(s) 1028
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 4 cut(s) 348, 594, 845, 1080
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 2 cut(s) 852, 1092
MspI CCGG 1 cut(s) 95
Mva1269I GAATGC 1 cut(s) 1207
MwoI GCNNNNNNNGC 5 cut(s) 24, 158, 218, 560, 815
NarI GGCGCC 1 cut(s) 25
NdeI CATATG 1 cut(s) 545
NdeII GATC 2 cut(s) 396, 907
NlaIII CATG 5 cut(s) 431, 809, 904, 986, 1118
NlaIV GGNNCC 4 cut(s) 26, 830, 859, 988
NmuCI GTSAC 1 cut(s) 205
PagI TCATGA 1 cut(s) 1114
PceI AGGCCT 1 cut(s) 87
PctI GAATGC 1 cut(s) 1207
PfeI GAWTC 4 cut(s) 119, 156, 472, 712
PkrI GCNGC 7 cut(s) 223, 256, 565, 610, 647, 655, 820
PleI GAGTC 1 cut(s) 1182
PluTI GGCGCC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 1182
PsiI TTATAA 1 cut(s) 1139
PspN4I GGNNCC 4 cut(s) 26, 830, 859, 988
PspPI GGNCC 3 cut(s) 362, 829, 987
PstI CTGCAG 2 cut(s) 613, 820
PstNI CAGNNNCTG 1 cut(s) 136
RsaI GTAC 1 cut(s) 1213
RsaNI GTAC 1 cut(s) 1212
SaqAI TTAA 4 cut(s) 348, 594, 845, 1080
SatI GCNGC 7 cut(s) 222, 255, 564, 609, 646, 654, 819
Sau3AI GATC 2 cut(s) 396, 907
Sau96I GGNCC 3 cut(s) 362, 829, 987
ScaI AGTACT 1 cut(s) 1213
SchI GAGTC 1 cut(s) 1183
SfaNI GCATC 1 cut(s) 139
SfcI CTRYAG 2 cut(s) 609, 816
SfoI GGCGCC 1 cut(s) 26
SinI GGWCC 2 cut(s) 362, 987
Sse9I AATT 8 cut(s) 267, 321, 626, 838, 963, 1021, 1081, 1097
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 7 cut(s) 12, 75, 241, 255, 645, 852, 1092
SspDI GGCGCC 1 cut(s) 24
SspMI CTAG 2 cut(s) 356, 758
StuI AGGCCT 1 cut(s) 87
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 1 cut(s) 487
TaiI ACGT 2 cut(s) 461, 814
TaqI TCGA 2 cut(s) 66, 1160
TasI AATT 8 cut(s) 267, 321, 626, 838, 963, 1021, 1081, 1097
TatI WGTACW 1 cut(s) 1211
TauI GCSGC 2 cut(s) 257, 648
TfiI GAWTC 4 cut(s) 119, 156, 472, 712
Tru1I TTAA 4 cut(s) 348, 594, 845, 1080
Tru9I TTAA 4 cut(s) 348, 594, 845, 1080
TscAI CASTG 1 cut(s) 293
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 5 cut(s) 221, 563, 608, 653, 818
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 4 cut(s) 272, 444, 929, 971
TspRI CASTG 1 cut(s) 293
VpaK11BI GGWCC 2 cut(s) 362, 987
XapI RAATTY 4 cut(s) 626, 963, 1021, 1097
XcmI CCANNNNNNNNNTGG 1 cut(s) 997
XspI CTAG 2 cut(s) 356, 758
ZrmI AGTACT 1 cut(s) 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.