MD08G1105900.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000265
Physical Location & Seq
Reverse (-)
9111797 .. 9114301
2505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1105900.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGAAAGAAGCGGTAGAGTAGAGCCCCCCACAGTCTTTGGAAAGATAGTCACAGTTCTCAGCATCGATGGGGGCGGAATAAGAGGCCTCATTCCGGCAACCATTCTTGCTTTCCTCGAATCTGAACTTCAGAAGCTGGATGGTGAAGATGCAAGGATCGCTGACTATTTTGATGTGATTGCAGGAACAAGCACAGGTGGTCTTGTGACCGCTATGCTCGCAGCCCCAAACGAGAATAACCGCCCACTGTTCGCTGCCAAAGATATTATAGACTTCTACTTAAATCTATGCCCTAAAATCTTCTCCCAAAACAGGTTGGGCAACAAAAGATTGCACGACACATTGACTAATGTTGTAATTCCCACATTTGACATCAAGAATCTCCAGCCAGCTATCTTCTCCAACTTTAAGGTGAAAAAGAAGCCATCATACGATGCCTTACTCTCTGACATATGCATTGGAACCTCAGCGGCACCAACTTATCTTCCAGCTCATTATTTTGAAACCAAGAACGCTGCAGGCAGAACTAGGGAATTCAACCTTATAGATGGCGGCATGGCTGCAAATAATCCGACTTTACTTGCTGTTTGTGAAGTTACAAACGAAATAAGGAAGGCGAATCCAGACGTTTTTCCTGTGAAACCTATGGACTATGGGAGATTTCTAGTGATATCATTAGGAACTGGTTCATCAAAAGCTGAAATGAAATACCATGCAAGCTCTTCAGCGAAATGGGGCCTGTTGAATTGGTTAACCAGCGGTGGCTCCACCCCCATCATTAATGCTTTCAGTCAAGCAAGCAACGATATGGTTGATCTTCACCTTTCTGCAATTTTCCAAGCCCTTCATTCTGAAGAAAATTATCTTAGAATTCAGGATGATACCCTTCGTGGGACAATAGCTTCTGTGGATATAGCCACAAAGAAGAATTTGACCAATCTTGTGAAAGTTGGAGAAGGGCTATTGAAAAAACCGGTTTCTCGGGTTAATTTGGACAGTGGCAGGTTTGAGGCTACTAAGCATGAGACAAATGCAGAGGCTCATACAAGGTTTGCTAAACTACTGTCGGAAGAGAAGAGGGGGCGCCAAGCAGGGTCAACCAACGGGCATACTGAAAGTACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

376

Amino Acids

40.88

Weight (kDa)

8.28

Isoelectric Point (pI)

26.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 20 - 195 1.3e-18 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 993
AccB1I GGYRCC 2 cut(s) 472, 1083
AciI CCGC 7 cut(s) 12, 75, 210, 241, 470, 552, 759
AclWI GGATC 1 cut(s) 164
AcsI RAATTY 3 cut(s) 533, 870, 928
AcuI CTGAAG 3 cut(s) 113, 708, 873
AcyI GRCGYC 1 cut(s) 1084
AfaI GTAC 1 cut(s) 1120
AfiI CCNNNNNNNGG 3 cut(s) 94, 312, 891
AgeI ACCGGT 1 cut(s) 973
AgsI TTSAA 4 cut(s) 503, 538, 745, 967
AluBI AGCT 6 cut(s) 136, 392, 491, 698, 720, 902
AluI AGCT 6 cut(s) 136, 392, 491, 698, 720, 902
Alw26I GTCTC 1 cut(s) 1019
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 981
AoxI GGCC 2 cut(s) 85, 736
ApeKI GCWGC 4 cut(s) 221, 254, 515, 560
ApoI RAATTY 3 cut(s) 533, 870, 928
AseI ATTAAT 1 cut(s) 780
AsiGI ACCGGT 1 cut(s) 973
Asp700I GAANNNNTTC 1 cut(s) 685
AspLEI GCGC 1 cut(s) 1086
AspS9I GGNCC 1 cut(s) 736
AsuHPI GGTGA 3 cut(s) 155, 424, 812
AvaI CYCGRG 1 cut(s) 981
BanI GGYRCC 2 cut(s) 472, 1083
BanII GRGCYC 1 cut(s) 27
BarI GAAGNNNNNNTAC 2 cut(s) 413, 445
BbvCI CCTCAGC 1 cut(s) 466
BbvI GCAGC 4 cut(s) 233, 241, 502, 547
BccI CCATC 5 cut(s) 62, 134, 433, 542, 782
BcoDI GTCTC 1 cut(s) 1019
BfaI CTAG 2 cut(s) 528, 665
BfmI CTRYAG 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 1087
BfuAI ACCTGC 1 cut(s) 993
BisI GCNGC 6 cut(s) 222, 255, 471, 516, 553, 561
BlsI GCNGC 6 cut(s) 223, 256, 472, 517, 554, 562
BmcAI AGTACT 1 cut(s) 1120
BmeT110I CYCGRG 1 cut(s) 981
BmgT120I GGNCC 1 cut(s) 736
BmiI GGNNCC 5 cut(s) 463, 474, 737, 766, 1085
BmsI GCATC 3 cut(s) 72, 139, 424
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 466
Bsa29I ATCGAT 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 1084
BsaWI WCCGGW 1 cut(s) 973
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 312, 891
Bse118I RCCGGY 1 cut(s) 973
Bse1I ACTGG 1 cut(s) 688
BseCI ATCGAT 1 cut(s) 66
BseGI GGATG 2 cut(s) 145, 883
BseLI CCNNNNNNNGG 3 cut(s) 94, 312, 891
BseMII CTCAG 2 cut(s) 73, 480
BseNI ACTGG 1 cut(s) 688
BseXI GCAGC 4 cut(s) 233, 241, 502, 547
BshFI GGCC 2 cut(s) 87, 738
BshNI GGYRCC 2 cut(s) 472, 1083
BshTI ACCGGT 1 cut(s) 973
BshVI ATCGAT 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 981
BsiSI CCGG 2 cut(s) 95, 974
BslFI GGGAC 1 cut(s) 907
BslI CCNNNNNNNGG 3 cut(s) 94, 312, 891
BsmAI GTCTC 1 cut(s) 1019
BsmFI GGGAC 1 cut(s) 907
BsnI GGCC 2 cut(s) 87, 738
BsoBI CYCGRG 1 cut(s) 981
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 156, 814
BspACI CCGC 7 cut(s) 12, 75, 210, 241, 470, 552, 759
BspANI GGCC 2 cut(s) 87, 738
BspCNI CTCAG 2 cut(s) 72, 479
BspDI ATCGAT 1 cut(s) 66
BspLI GGNNCC 5 cut(s) 463, 474, 737, 766, 1085
BspMAI CTGCAG 1 cut(s) 520
BspMI ACCTGC 1 cut(s) 993
BspPI GGATC 1 cut(s) 164
BspQI GCTCTTC 1 cut(s) 727
BspT107I GGYRCC 2 cut(s) 472, 1083
BsrFI RCCGGY 1 cut(s) 973
BsrI ACTGG 1 cut(s) 688
BssAI RCCGGY 1 cut(s) 973
BssMI GATC 2 cut(s) 156, 814
BssNI GRCGYC 1 cut(s) 1084
Bst4CI ACNGT 5 cut(s) 34, 55, 249, 998, 1065
Bst6I CTCTTC 3 cut(s) 727, 1065, 1070
BstACI GRCGYC 1 cut(s) 1084
BstC8I GCNNGC 5 cut(s) 219, 390, 520, 718, 799
BstDEI CTNAG 4 cut(s) 59, 466, 866, 1017
BstF5I GGATG 2 cut(s) 145, 883
BstH2I RGCGCY 1 cut(s) 1087
BstHHI GCGC 1 cut(s) 1086
BstKTI GATC 2 cut(s) 159, 817
BstMAI GTCTC 1 cut(s) 1019
BstMBI GATC 2 cut(s) 156, 814
BstMWI GCNNNNNNNGC 2 cut(s) 158, 218
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 4 cut(s) 233, 241, 502, 547
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 2 cut(s) 87, 738
BsuTUI ATCGAT 1 cut(s) 66
BtsCI GGATG 2 cut(s) 145, 883
BtsIMutI CAGTG 2 cut(s) 245, 1003
BveI ACCTGC 1 cut(s) 993
Cac8I GCNNGC 5 cut(s) 219, 390, 520, 718, 799
CaiI CAGNNNCTG 1 cut(s) 136
CfoI GCGC 1 cut(s) 1086
Cfr10I RCCGGY 1 cut(s) 973
Cfr13I GGNCC 1 cut(s) 736
ClaI ATCGAT 1 cut(s) 66
Csp6I GTAC 1 cut(s) 1119
CspAI ACCGGT 1 cut(s) 973
CspCI CAANNNNNGTGG 2 cut(s) 19, 54
CviAII CATG 3 cut(s) 556, 713, 1022
CviQI GTAC 1 cut(s) 1119
DdeI CTNAG 4 cut(s) 59, 466, 866, 1017
DinI GGCGCC 1 cut(s) 1085
DpnI GATC 2 cut(s) 158, 816
DpnII GATC 2 cut(s) 156, 814
Eam1104I CTCTTC 3 cut(s) 727, 1065, 1070
EarI CTCTTC 3 cut(s) 727, 1065, 1070
EciI GGCGGA 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 27
Eco32I GATATC 1 cut(s) 672
Eco57I CTGAAG 3 cut(s) 113, 708, 873
Eco88I CYCGRG 1 cut(s) 981
EcoO109I RGGNCCY 1 cut(s) 736
EcoRI GAATTC 2 cut(s) 533, 870
EcoRV GATATC 1 cut(s) 672
EcoT22I ATGCAT 1 cut(s) 458
EcoT38I GRGCYC 1 cut(s) 27
EgeI GGCGCC 1 cut(s) 1085
EheI GGCGCC 1 cut(s) 1085
FaeI CATG 3 cut(s) 559, 716, 1025
FaqI GGGAC 1 cut(s) 907
FatI CATG 3 cut(s) 555, 712, 1021
FauNDI CATATG 1 cut(s) 452
Fnu4HI GCNGC 6 cut(s) 222, 255, 471, 516, 553, 561
FokI GGATG 2 cut(s) 152, 890
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 6 cut(s) 222, 255, 471, 516, 553, 561
FspBI CTAG 2 cut(s) 528, 665
GlaI GCGC 1 cut(s) 1085
GluI GCNGC 6 cut(s) 222, 255, 471, 516, 553, 561
GsuI CTGGAG 1 cut(s) 368
HaeII RGCGCY 1 cut(s) 1087
HaeIII GGCC 2 cut(s) 87, 738
HapII CCGG 2 cut(s) 95, 974
HhaI GCGC 1 cut(s) 1086
Hin1I GRCGYC 1 cut(s) 1084
Hin1II CATG 3 cut(s) 559, 716, 1025
Hin6I GCGC 1 cut(s) 1084
HinP1I GCGC 1 cut(s) 1084
HincII GTYRAC 2 cut(s) 753, 1098
HindII GTYRAC 2 cut(s) 753, 1098
HinfI GANTC 3 cut(s) 119, 379, 619
HpaI GTTAAC 1 cut(s) 753
HpaII CCGG 2 cut(s) 95, 974
HphI GGTGA 3 cut(s) 155, 424, 812
Hpy166II GTNNAC 2 cut(s) 753, 1098
Hpy188I TCNGA 6 cut(s) 124, 132, 448, 573, 853, 1069
Hpy188III TCNNGA 3 cut(s) 376, 623, 875
Hpy8I GTNNAC 2 cut(s) 753, 1098
HpyAV CCTTC 4 cut(s) 607, 854, 896, 950
HpyCH4III ACNGT 5 cut(s) 34, 55, 249, 998, 1065
HpyCH4IV ACGT 1 cut(s) 627
HpyCH4V TGCA 9 cut(s) 152, 182, 334, 456, 518, 563, 716, 830, 1034
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 218
HpyF3I CTNAG 4 cut(s) 59, 466, 866, 1017
HpySE526I ACGT 1 cut(s) 627
Hsp92I GRCGYC 1 cut(s) 1084
Hsp92II CATG 3 cut(s) 559, 716, 1025
HspAI GCGC 1 cut(s) 1084
KasI GGCGCC 1 cut(s) 1083
KspAI GTTAAC 1 cut(s) 753
Kzo9I GATC 2 cut(s) 156, 814
LguI GCTCTTC 1 cut(s) 727
LmnI GCTCC 1 cut(s) 770
Lsp1109I GCAGC 4 cut(s) 233, 241, 502, 547
LweI GCATC 3 cut(s) 72, 139, 424
MaeI CTAG 2 cut(s) 528, 665
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 3 cut(s) 49, 205, 595
MalI GATC 2 cut(s) 158, 816
MboI GATC 2 cut(s) 156, 814
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 8 cut(s) 357, 533, 745, 831, 859, 870, 928, 988
Mly113I GGCGCC 1 cut(s) 1084
MmeI TCCRAC 4 cut(s) 426, 596, 931, 1047
MnlI CCTC 7 cut(s) 77, 98, 125, 475, 1003, 1030, 1071
Mph1103I ATGCAT 1 cut(s) 458
MroXI GAANNNNTTC 1 cut(s) 685
MseI TTAA 5 cut(s) 281, 408, 752, 780, 987
MspA1I CMGCKG 2 cut(s) 470, 759
MspI CCGG 2 cut(s) 95, 974
MwoI GCNNNNNNNGC 2 cut(s) 158, 218
NarI GGCGCC 1 cut(s) 1084
NdeI CATATG 1 cut(s) 452
NdeII GATC 2 cut(s) 156, 814
NlaIII CATG 3 cut(s) 559, 716, 1025
NlaIV GGNNCC 5 cut(s) 463, 474, 737, 766, 1085
NmuCI GTSAC 2 cut(s) 49, 205
NsiI ATGCAT 1 cut(s) 458
PceI AGGCCT 1 cut(s) 87
PciSI GCTCTTC 1 cut(s) 727
PdmI GAANNNNTTC 1 cut(s) 685
PfeI GAWTC 3 cut(s) 119, 379, 619
PinAI ACCGGT 1 cut(s) 973
PkrI GCNGC 6 cut(s) 223, 256, 472, 517, 554, 562
PluTI GGCGCC 1 cut(s) 1087
PshBI ATTAAT 1 cut(s) 780
PspN4I GGNNCC 5 cut(s) 463, 474, 737, 766, 1085
PspPI GGNCC 1 cut(s) 736
PstI CTGCAG 1 cut(s) 520
PstNI CAGNNNCTG 1 cut(s) 136
RsaI GTAC 1 cut(s) 1120
RsaNI GTAC 1 cut(s) 1119
SapI GCTCTTC 1 cut(s) 727
SaqAI TTAA 5 cut(s) 281, 408, 752, 780, 987
SatI GCNGC 6 cut(s) 222, 255, 471, 516, 553, 561
Sau3AI GATC 2 cut(s) 156, 814
Sau96I GGNCC 1 cut(s) 736
ScaI AGTACT 1 cut(s) 1120
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 3 cut(s) 72, 139, 424
SfcI CTRYAG 1 cut(s) 516
SfoI GGCGCC 1 cut(s) 1085
Sse9I AATT 8 cut(s) 357, 533, 745, 831, 859, 870, 928, 988
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 7 cut(s) 12, 75, 210, 241, 470, 552, 759
SspDI GGCGCC 1 cut(s) 1083
SspMI CTAG 2 cut(s) 528, 665
StuI AGGCCT 1 cut(s) 87
TaaI ACNGT 5 cut(s) 34, 55, 249, 998, 1065
TaiI ACGT 1 cut(s) 630
TaqI TCGA 2 cut(s) 66, 117
TasI AATT 8 cut(s) 357, 533, 745, 831, 859, 870, 928, 988
TatI WGTACW 1 cut(s) 1118
TauI GCSGC 2 cut(s) 473, 555
TfiI GAWTC 3 cut(s) 119, 379, 619
Tru1I TTAA 5 cut(s) 281, 408, 752, 780, 987
Tru9I TTAA 5 cut(s) 281, 408, 752, 780, 987
TscAI CASTG 2 cut(s) 252, 1003
TseFI GTSAC 2 cut(s) 49, 205
TseI GCWGC 4 cut(s) 221, 254, 515, 560
Tsp45I GTSAC 2 cut(s) 49, 205
TspDTI ATGAA 3 cut(s) 678, 719, 836
TspRI CASTG 2 cut(s) 252, 1003
VspI ATTAAT 1 cut(s) 780
XapI RAATTY 3 cut(s) 533, 870, 928
XmnI GAANNNNTTC 1 cut(s) 685
XspI CTAG 2 cut(s) 528, 665
ZrmI AGTACT 1 cut(s) 1120
Zsp2I ATGCAT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.