Rroxscaffold_2G00138670

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000265
Physical Location & Seq
Forward (+)
76842568 .. 76843415
848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00138670.1

Sequence Viewer

Length: 444 bp
ATGTTGAAGAACGGCTCAGAACCAATGGATGCTAATCGGTTGCTAGTGATATCATTGGGCACAGGCACTGCCAAGTTCGAAGGGAAGTACAGTGCAACCACGGCGTCAAAATGGGGTTTGATCAATTGGATGTTTGACAATGGGAGCACACCTATGATCGATATCTTTACCGATGCAAGTTCGGATATGGTTGACATTCATGTCTCCACTCTCTTTCAGACTTGTTTGCTTCTTGTTAATACTTTTGCTGTGGATATTGCAACGGAGAAAAATCTGAAGAGGCTTGTGGAGATCGGGAATGGGCTGTTGAAAAAGCCGGTGTCAAGGGTAAATTTGGATAACGGGAGGTATGAGAAATCTGAAGGAGAAGGTACCTATGAAGAAGCACTGGTCGATTTTGCCAAACGGCTCGTGGAGGGGAAGAAGCACAGGCAATACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

147

Amino Acids

16.37

Weight (kDa)

6.74

Isoelectric Point (pI)

14.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
Acc65I GGTACC 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 371
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 2 cut(s) 296, 381
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 2 cut(s) 89, 373
AgsI TTSAA 2 cut(s) 7, 310
Alw21I GWGCWC 1 cut(s) 149
Alw26I GTCTC 1 cut(s) 208
AlwNI CAGNNNCTG 1 cut(s) 68
ApoI RAATTY 1 cut(s) 331
Asp718I GGTACC 1 cut(s) 371
AsuII TTCGAA 1 cut(s) 78
BaeGI GKGCMC 1 cut(s) 62
BanI GGYRCC 1 cut(s) 371
BauI CACGAG 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 149
BceAI ACGGC 3 cut(s) 28, 117, 422
BclI TGATCA 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 208
BfaI CTAG 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 373
BmsI GCATC 2 cut(s) 19, 163
Bpu14I TTCGAA 1 cut(s) 78
Bsa29I ATCGAT 1 cut(s) 159
BsaBI GATNNNNATC 2 cut(s) 33, 161
BsaHI GRCGYC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 99
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 393
Bse8I GATNNNNATC 2 cut(s) 33, 161
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 2 cut(s) 34, 135
BseJI GATNNNNATC 2 cut(s) 33, 161
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 1 cut(s) 393
BseSI GKGCMC 1 cut(s) 62
BshNI GGYRCC 1 cut(s) 371
BshVI ATCGAT 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 149
BsiSI CCGG 1 cut(s) 317
BsmAI GTCTC 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 62, 149
Bsp143I GATC 3 cut(s) 120, 156, 291
BspCNI CTCAG 1 cut(s) 29
BspDI ATCGAT 1 cut(s) 159
BspLI GGNNCC 1 cut(s) 373
BspT104I TTCGAA 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 316
BsrI ACTGG 1 cut(s) 393
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 3 cut(s) 120, 156, 291
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 410
Bst2BI CACGAG 1 cut(s) 410
Bst4CI ACNGT 1 cut(s) 92
Bst6I CTCTTC 1 cut(s) 272
BstACI GRCGYC 1 cut(s) 104
BstBI TTCGAA 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 16
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 2 cut(s) 34, 135
BstKTI GATC 3 cut(s) 123, 159, 294
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 3 cut(s) 120, 156, 291
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstSLI GKGCMC 1 cut(s) 62
Bsu15I ATCGAT 1 cut(s) 159
BsuTUI ATCGAT 1 cut(s) 159
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 2 cut(s) 34, 135
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 3 cut(s) 66, 97, 386
CaiI CAGNNNCTG 1 cut(s) 68
Cfr10I RCCGGY 1 cut(s) 316
ClaI ATCGAT 1 cut(s) 159
CseI GACGC 1 cut(s) 93
Csp6I GTAC 2 cut(s) 88, 372
CviAII CATG 1 cut(s) 200
CviJI RGCY 5 cut(s) 15, 283, 304, 316, 409
CviKI_1 RGCY 5 cut(s) 15, 283, 304, 316, 409
CviQI GTAC 2 cut(s) 88, 372
DdeI CTNAG 1 cut(s) 16
DpnI GATC 3 cut(s) 122, 158, 293
DpnII GATC 3 cut(s) 120, 156, 291
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
Eco32I GATATC 2 cut(s) 51, 163
Eco57I CTGAAG 2 cut(s) 296, 381
EcoRV GATATC 2 cut(s) 51, 163
FaeI CATG 1 cut(s) 203
FaiI YATR 5 cut(s) 155, 188, 201, 351, 378
FatI CATG 1 cut(s) 199
FbaI TGATCA 1 cut(s) 120
FokI GGATG 2 cut(s) 41, 142
FspBI CTAG 1 cut(s) 44
HapII CCGG 1 cut(s) 317
HgaI GACGC 1 cut(s) 93
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 1 cut(s) 203
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HpaII CCGG 1 cut(s) 317
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 5 cut(s) 19, 184, 219, 276, 361
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 3 cut(s) 74, 356, 362
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4V TGCA 3 cut(s) 95, 176, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 1 cut(s) 16
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 1 cut(s) 203
KpnI GGTACC 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 120
Kzo9I GATC 3 cut(s) 120, 156, 291
LmnI GCTCC 1 cut(s) 144
LpnPI CCDG 4 cut(s) 48, 330, 374, 415
LweI GCATC 2 cut(s) 19, 163
MaeI CTAG 1 cut(s) 44
MalI GATC 3 cut(s) 122, 158, 293
MboI GATC 3 cut(s) 120, 156, 291
MboII GAAGA 4 cut(s) 19, 289, 392, 433
MfeI CAATTG 1 cut(s) 124
MhlI GDGCHC 2 cut(s) 62, 149
MluCI AATT 2 cut(s) 124, 331
MnlI CCTC 3 cut(s) 273, 339, 409
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 152
MspI CCGG 1 cut(s) 317
MunI CAATTG 1 cut(s) 124
MwoI GCNNNNNNNGC 1 cut(s) 101
NdeII GATC 3 cut(s) 120, 156, 291
NlaIII CATG 1 cut(s) 203
NlaIV GGNNCC 1 cut(s) 373
NspV TTCGAA 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 68
RsaI GTAC 2 cut(s) 89, 373
RsaNI GTAC 2 cut(s) 88, 372
RseI CAYNNNNRTG 1 cut(s) 152
SaqAI TTAA 1 cut(s) 237
Sau3AI GATC 3 cut(s) 120, 156, 291
SduI GDGCHC 2 cut(s) 62, 149
SetI ASST 4 cut(s) 154, 350, 373, 377
SfaNI GCATC 2 cut(s) 19, 163
SfuI TTCGAA 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 152
Sse9I AATT 2 cut(s) 124, 331
SspMI CTAG 1 cut(s) 44
TaaI ACNGT 1 cut(s) 92
TaqI TCGA 3 cut(s) 78, 159, 393
TasI AATT 2 cut(s) 124, 331
TatI WGTACW 1 cut(s) 87
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 3 cut(s) 73, 97, 393
TspDTI ATGAA 2 cut(s) 188, 393
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 3 cut(s) 73, 97, 393
XapI RAATTY 1 cut(s) 331
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.