RchiOBHm_Chr6g0307661

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000265
Physical Location & Seq
Forward (+)
65928299 .. 65930180
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27660

Sequence Viewer

Length: 1035 bp
ATGGAAAGAACTGGTAGTATCGCTTTACAGCCCCCAACCTTTGGAAACCTAATCACTGTTCTGAGCATCGACGGCGGTGGAATAAGAGGCTTATCCCAGGAACCATCCTTGCTTTCCTTGAGTCTGAACTTCAGACTCGCCGACTATTTCGATGTGATTTCAGGGACAAGCACAGGTGGTCTCGTAACTGCCATGCTCACAGCCCAAATGAGAATGACCGTCCACTATTTGCTGCCAAAGATATCAAGGACTTCTACTGACCCACTCCCCCAAATCTTCCCCCAGAAGACTGGTTGGCTGTTTCCTCGTGCTAGAAAGATCATCAGAGCTCTAGCAGGACCGAAATACGATGGGAAGTATCTACATGGCTTGGTTAGGGAAAAGCTTGGTGACAAAAAACTGAATCAAACATTGACTAATGTTGTCATTCCAGCATTTGACATAAAGAATCTCCAGCCAGCTATATTTTCCAGCTTCAAGACTTTGATTGCAATTGGTGAAGTGACAAAGGAATTAATTAAGGGGAGTTCAGACTTCTTTCCTATAAAACCAATGGACTATGGAAGATTTCTGGTGATATCCTTAGGAACTGGCTCATCGAAAGCTGAACTGAAATACAATGCTCATGTTGCCTCCAAGTGGGGTGTGTTGAATTGGTTAACGAGCGGTGGTTCCACCCCATTAATCAATGCTTTCAGTCAAGCAAGTGCCGATATGGTCGATTTGCATCTTTCTGTGGTTTTCAAAGCCCTTCACTCGGAGAAAAACTATCTTCGAATTCAGGATGACACGCTAAGCGGCGAAGTATCTTCCGTAGATATAGCAACACAGAAGAATCTGGACCATCTTCTCAGAGTTGGTGATGGACTATTGAACCAACCAGTTTCCAGGGTTAATTTAGAGACAGGCAAATTCGAAGCTTGTAATCATGAGACTAATAAAGAGGCTCTTATAAGGTTTGCAAAATTGCTGTCCGAAGAGAAGTGGCTTCGCCTTGCAAGGACTCCCCATGGACATATTGCAAAGTCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

344

Amino Acids

37.85

Weight (kDa)

9.55

Isoelectric Point (pI)

24.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 21 - 152 9.4e-12 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
AccB7I CCANNNNNTGG 1 cut(s) 41
AccBSI CCGCTC 1 cut(s) 666
AciI CCGC 3 cut(s) 75, 666, 798
AcsI RAATTY 2 cut(s) 777, 911
AcuI CTGAAG 1 cut(s) 115
AfiI CCNNNNNNNGG 3 cut(s) 41, 639, 757
AgsI TTSAA 4 cut(s) 478, 652, 745, 874
AjnI CCWGG 2 cut(s) 96, 887
AluBI AGCT 6 cut(s) 329, 385, 461, 474, 605, 920
AluI AGCT 6 cut(s) 329, 385, 461, 474, 605, 920
Alw21I GWGCWC 1 cut(s) 331
Alw26I GTCTC 3 cut(s) 185, 896, 926
ApeKI GCWGC 1 cut(s) 232
ApoI RAATTY 2 cut(s) 777, 911
ArsI GACNNNNNNTTYG 2 cut(s) 956, 988
AseI ATTAAT 2 cut(s) 515, 683
AspS9I GGNCC 2 cut(s) 338, 841
AsuHPI GGTGA 4 cut(s) 401, 509, 586, 872
AsuII TTCGAA 2 cut(s) 775, 915
AvaII GGWCC 2 cut(s) 338, 841
AxyI CCTNAGG 1 cut(s) 583
BanII GRGCYC 1 cut(s) 331
BauI CACGAG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 1 cut(s) 219
BccI CCATC 4 cut(s) 112, 344, 852, 857
BceAI ACGGC 1 cut(s) 88
BciT130I CCWGG 2 cut(s) 98, 889
BcoDI GTCTC 3 cut(s) 185, 896, 926
BfaI CTAG 2 cut(s) 312, 332
BisI GCNGC 2 cut(s) 233, 799
BlpI GCTNAGC 1 cut(s) 794
BlsI GCNGC 2 cut(s) 234, 800
Bme1390I CCNGG 2 cut(s) 98, 889
Bme18I GGWCC 2 cut(s) 338, 841
BmgT120I GGNCC 2 cut(s) 338, 841
BmiI GGNNCC 2 cut(s) 102, 673
BmrFI CCNGG 2 cut(s) 98, 889
BmsI GCATC 2 cut(s) 75, 736
BpiI GAAGAC 1 cut(s) 293
BpmI CTGGAG 1 cut(s) 437
Bpu1102I GCTNAGC 1 cut(s) 794
Bpu14I TTCGAA 2 cut(s) 775, 915
BpuEI CTTGAG 1 cut(s) 139
BsaBI GATNNNNATC 1 cut(s) 726
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 3 cut(s) 96, 888, 1009
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 639, 757
Bse1I ACTGG 4 cut(s) 16, 295, 595, 881
Bse21I CCTNAGG 1 cut(s) 583
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 2 cut(s) 98, 889
BseDI CCNNGG 3 cut(s) 96, 888, 1009
BseGI GGATG 2 cut(s) 104, 790
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 3 cut(s) 41, 639, 757
BseMII CTCAG 2 cut(s) 53, 865
BseNI ACTGG 4 cut(s) 16, 295, 595, 881
BseXI GCAGC 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 331
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 3 cut(s) 41, 639, 757
BsmAI GTCTC 3 cut(s) 185, 896, 926
BsmFI GGGAC 1 cut(s) 178
Bso31I GGTCTC 1 cut(s) 185
Bsp119I TTCGAA 2 cut(s) 775, 915
Bsp1286I GDGCHC 1 cut(s) 331
Bsp143I GATC 1 cut(s) 318
Bsp1720I GCTNAGC 1 cut(s) 794
Bsp19I CCATGG 1 cut(s) 1009
BspACI CCGC 3 cut(s) 75, 666, 798
BspCNI CTCAG 2 cut(s) 54, 864
BspHI TCATGA 1 cut(s) 928
BspLI GGNNCC 2 cut(s) 102, 673
BspT104I TTCGAA 2 cut(s) 775, 915
BspTNI GGTCTC 1 cut(s) 185
BsrBI CCGCTC 1 cut(s) 666
BsrI ACTGG 4 cut(s) 16, 295, 595, 881
BssECI CCNNGG 3 cut(s) 96, 888, 1009
BssMI GATC 1 cut(s) 318
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 1009
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 98, 889
Bst4CI ACNGT 2 cut(s) 58, 220
Bst6I CTCTTC 1 cut(s) 972
BstBI TTCGAA 2 cut(s) 775, 915
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 4 cut(s) 62, 583, 794, 851
BstDSI CCRYGG 1 cut(s) 1009
BstF5I GGATG 2 cut(s) 104, 790
BstKTI GATC 1 cut(s) 321
BstMAI GTCTC 3 cut(s) 185, 896, 926
BstMBI GATC 1 cut(s) 318
BstMWI GCNNNNNNNGC 2 cut(s) 72, 629
BstNI CCWGG 2 cut(s) 98, 889
BstSCI CCNGG 2 cut(s) 96, 887
BstV1I GCAGC 1 cut(s) 219
BstV2I GAAGAC 1 cut(s) 293
BstXI CCANNNNNNTGG 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 583
BtgI CCRYGG 1 cut(s) 1009
BtsCI GGATG 2 cut(s) 104, 790
BtsIMutI CAGTG 2 cut(s) 54, 1030
Cac8I GCNNGC 1 cut(s) 459
CciI TCATGA 1 cut(s) 928
Cfr13I GGNCC 2 cut(s) 338, 841
CviAII CATG 5 cut(s) 193, 365, 626, 929, 1010
DdeI CTNAG 4 cut(s) 62, 583, 794, 851
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 972
EarI CTCTTC 1 cut(s) 972
Ecl136II GAGCTC 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 1009
Eco24I GRGCYC 1 cut(s) 331
Eco31I GGTCTC 1 cut(s) 185
Eco32I GATATC 2 cut(s) 243, 579
Eco47I GGWCC 2 cut(s) 338, 841
Eco53kI GAGCTC 1 cut(s) 329
Eco57I CTGAAG 1 cut(s) 115
Eco81I CCTNAGG 1 cut(s) 583
EcoICRI GAGCTC 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 777
EcoRII CCWGG 2 cut(s) 96, 887
EcoRV GATATC 2 cut(s) 243, 579
EcoT14I CCWWGG 1 cut(s) 1009
EcoT38I GRGCYC 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 1009
FaeI CATG 5 cut(s) 196, 368, 629, 932, 1013
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 1 cut(s) 178
FatI CATG 5 cut(s) 192, 364, 625, 928, 1009
Fnu4HI GCNGC 2 cut(s) 233, 799
FokI GGATG 2 cut(s) 91, 797
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 2 cut(s) 233, 799
FspBI CTAG 2 cut(s) 312, 332
GluI GCNGC 2 cut(s) 233, 799
GsuI CTGGAG 1 cut(s) 437
Hin1II CATG 5 cut(s) 196, 368, 629, 932, 1013
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HindIII AAGCTT 2 cut(s) 383, 918
HinfI GANTC 6 cut(s) 121, 135, 403, 448, 835, 1003
HpaI GTTAAC 1 cut(s) 660
HphI GGTGA 4 cut(s) 401, 509, 586, 872
Hpy166II GTNNAC 2 cut(s) 223, 660
Hpy188I TCNGA 8 cut(s) 63, 126, 134, 326, 532, 760, 854, 976
Hpy188III TCNNGA 4 cut(s) 478, 782, 839, 929
Hpy8I GTNNAC 2 cut(s) 223, 660
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 761
HpyCH4III ACNGT 2 cut(s) 58, 220
HpyCH4V TGCA 5 cut(s) 491, 727, 962, 998, 1022
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 629
HpyF3I CTNAG 4 cut(s) 62, 583, 794, 851
Hsp92II CATG 5 cut(s) 196, 368, 629, 932, 1013
KspAI GTTAAC 1 cut(s) 660
Kzo9I GATC 1 cut(s) 318
Lsp1109I GCAGC 1 cut(s) 219
LweI GCATC 2 cut(s) 75, 736
MaeI CTAG 2 cut(s) 312, 332
MaeIII GTNAC 3 cut(s) 184, 389, 502
MalI GATC 1 cut(s) 320
MbiI CCGCTC 1 cut(s) 666
MboI GATC 1 cut(s) 318
MboII GAAGA 8 cut(s) 268, 298, 576, 764, 801, 839, 844, 989
MfeI CAATTG 1 cut(s) 492
MhlI GDGCHC 1 cut(s) 331
MluCI AATT 8 cut(s) 492, 512, 516, 652, 777, 895, 911, 965
MlyI GAGTC 3 cut(s) 129, 130, 997
MnlI CCTC 4 cut(s) 80, 315, 643, 937
MseI TTAA 5 cut(s) 515, 519, 659, 683, 894
MspR9I CCNGG 2 cut(s) 98, 889
MunI CAATTG 1 cut(s) 492
MvaI CCWGG 2 cut(s) 98, 889
MwoI GCNNNNNNNGC 2 cut(s) 72, 629
NcoI CCATGG 1 cut(s) 1009
NdeII GATC 1 cut(s) 318
NlaIII CATG 5 cut(s) 196, 368, 629, 932, 1013
NlaIV GGNNCC 2 cut(s) 102, 673
NmuCI GTSAC 2 cut(s) 389, 502
NspV TTCGAA 2 cut(s) 775, 915
PacI TTAATTAA 1 cut(s) 519
PagI TCATGA 1 cut(s) 928
PfeI GAWTC 3 cut(s) 403, 448, 835
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 2 cut(s) 234, 800
PleI GAGTC 3 cut(s) 129, 129, 997
PpsI GAGTC 3 cut(s) 129, 129, 997
PshBI ATTAAT 2 cut(s) 515, 683
PsiI TTATAA 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 96, 887
PspGI CCWGG 2 cut(s) 96, 887
PspN4I GGNNCC 2 cut(s) 102, 673
PspPI GGNCC 2 cut(s) 338, 841
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 5 cut(s) 515, 519, 659, 683, 894
SatI GCNGC 2 cut(s) 233, 799
Sau3AI GATC 1 cut(s) 318
Sau96I GGNCC 2 cut(s) 338, 841
SchI GAGTC 3 cut(s) 129, 130, 997
ScrFI CCNGG 2 cut(s) 98, 889
SduI GDGCHC 1 cut(s) 331
SfaNI GCATC 2 cut(s) 75, 736
SfuI TTCGAA 2 cut(s) 775, 915
SinI GGWCC 2 cut(s) 338, 841
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 8 cut(s) 492, 512, 516, 652, 777, 895, 911, 965
SsiI CCGC 3 cut(s) 75, 666, 798
SspMI CTAG 2 cut(s) 312, 332
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 2 cut(s) 96, 887
StyI CCWWGG 1 cut(s) 1009
TaaI ACNGT 2 cut(s) 58, 220
TaqI TCGA 6 cut(s) 69, 150, 599, 720, 775, 915
TaqII GACCGA 1 cut(s) 355
TasI AATT 8 cut(s) 492, 512, 516, 652, 777, 895, 911, 965
TauI GCSGC 1 cut(s) 801
TfiI GAWTC 3 cut(s) 403, 448, 835
Tru1I TTAA 5 cut(s) 515, 519, 659, 683, 894
Tru9I TTAA 5 cut(s) 515, 519, 659, 683, 894
TscAI CASTG 1 cut(s) 61
TseFI GTSAC 2 cut(s) 389, 502
TseI GCWGC 1 cut(s) 232
Tsp45I GTSAC 2 cut(s) 389, 502
TspGWI ACGGA 1 cut(s) 802
TspRI CASTG 1 cut(s) 61
Van91I CCANNNNNTGG 1 cut(s) 41
VpaK11BI GGWCC 2 cut(s) 338, 841
VspI ATTAAT 2 cut(s) 515, 683
XapI RAATTY 2 cut(s) 777, 911
XspI CTAG 2 cut(s) 312, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.