Rorug06G0357900

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000265
Physical Location & Seq
Forward (+)
50630588 .. 50634103
3516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0357900.1

Sequence Viewer

Length: 1032 bp
ATGGGTGATGACTTAAATGCACTGAATGCTTTGAAATGTGGAATTTTCTTATGTGTTGCTTTTGGGTTTTTTGTAGTTGATGAATCCAGCGTCGGGCAATCCAAGGAGCTCAATGATGCTGTTAAGGCTAAATTTATAGAGGAATATCCAGAGGCTTTGATTCAGACCATCCCCTCCTTTTCTCAGTTTACCTTGGTTGTGGCCACTCAGCTGGTGGAAGATTCAATGGTGAAGCTCGGCAGAATATGTAGGGATGCAAGTGTGCTATTGATCTTTGCTCGCTCTTATGGCCTCACTGGGCTTGTCCGTATCAGTTTGAAGGAACATACTGTGATTGAGTCCAAGCCTGAACATTTCCTGGATGACCTTCATTTGAATAATCCATGGCCTGAGCTTAGAGGGTACAGAGGTTCCGTCGTCTCCAATCTCAGCAAAGTTCTCACCATGTCGAAAAACATAAAATTCAGCGTTTACGTAGGTAATTTGGACGAGCGGGTGATTGATAGAGTTTTATATGATATTCTAATCCAAGCAGGGAGGGTAGTTGACCTGCACATTCCACGAGACAAGGAAAGTGATAGGCCTAAAGGTTATGCTTTTGCAGAATATGAAACTGAAGAGATTGCTGAGTATGCTGTTAGGCTTTTCTCCGGTCTTGTGACTCTCTACAATCAAACACTCAAGTTTGCGATTTCTGGGCAAGACGAGCCTTCACCTAATGTTGCTTCTACAGTAATGCCCACAACCAATTTTTCTAACAAATTGAGGTCTCCCCCTGTACCTATTAACAATGTAGAAAACTTGCAGAATTCAATGAGCTTATCAGCACCCTGCAAAATTCCAGCTTATGCAGAAACTTATCCCTCAGTGCCCATTCCCCCTGGTGTAAATAACCAATCTAATGGATTACATTTGAATGGCAACAATTTCGAGTATAGTCGAAGAGTTATTGGGGCAACATTGGATACCATTAGTCTCCCAAGGTCACGCCGCTATGATATGAGTAATCCAATTTCCTATCCGTCCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

343

Amino Acids

38.21

Weight (kDa)

5.66

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 157 - 227 2.2e-12 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 558
AccBSI CCGCTC 1 cut(s) 493
AciI CCGC 2 cut(s) 493, 991
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 5 cut(s) 42, 131, 461, 808, 837
AcuI CTGAAG 1 cut(s) 636
AfaI GTAC 2 cut(s) 404, 780
AfiI CCNNNNNNNGG 1 cut(s) 93
AgsI TTSAA 6 cut(s) 34, 225, 319, 376, 813, 916
AjnI CCWGG 2 cut(s) 357, 880
AluBI AGCT 6 cut(s) 109, 211, 235, 394, 819, 845
AluI AGCT 6 cut(s) 109, 211, 235, 394, 819, 845
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 4 cut(s) 424, 558, 774, 980
AoxI GGCC 4 cut(s) 201, 289, 386, 581
ApoI RAATTY 5 cut(s) 42, 131, 461, 808, 837
AsuHPI GGTGA 5 cut(s) 17, 241, 433, 508, 705
BaeGI GKGCMC 1 cut(s) 873
BaeI ACNNNNGTAYC 2 cut(s) 394, 427
BalI TGGCCA 1 cut(s) 203
BanII GRGCYC 1 cut(s) 111
BauI CACGAG 1 cut(s) 561
Bbv12I GWGCWC 1 cut(s) 111
BccI CCATC 1 cut(s) 176
BcgI CGANNNNNNTGC 2 cut(s) 910, 944
BciT130I CCWGG 2 cut(s) 359, 882
BciVI GTATCC 1 cut(s) 958
BcoDI GTCTC 4 cut(s) 424, 558, 774, 980
BfmI CTRYAG 1 cut(s) 729
BfuAI ACCTGC 1 cut(s) 558
BfuI GTATCC 1 cut(s) 958
BisI GCNGC 1 cut(s) 991
BlsI GCNGC 1 cut(s) 992
Bme1390I CCNGG 2 cut(s) 359, 882
BmiI GGNNCC 1 cut(s) 412
BmrFI CCNGG 2 cut(s) 359, 882
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 2 cut(s) 106, 244
BmuI ACTGGG 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 665
BsaAI YACGTR 1 cut(s) 475
BsaI GGTCTC 1 cut(s) 774
BsaJI CCNNGG 5 cut(s) 102, 192, 383, 880, 980
BsaWI WCCGGW 1 cut(s) 650
Bsc4I CCNNNNNNNGG 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 301
BseBI CCWGG 2 cut(s) 359, 882
BseDI CCNNGG 5 cut(s) 102, 192, 383, 880, 980
BseGI GGATG 3 cut(s) 168, 259, 367
BseLI CCNNNNNNNGG 1 cut(s) 93
BseMII CTCAG 6 cut(s) 197, 221, 381, 442, 618, 879
BseNI ACTGG 1 cut(s) 301
BseSI GKGCMC 1 cut(s) 873
BsgI GTGCAG 1 cut(s) 536
BshFI GGCC 4 cut(s) 203, 291, 388, 583
BsiHKAI GWGCWC 1 cut(s) 111
BsiSI CCGG 1 cut(s) 651
BslI CCNNNNNNNGG 1 cut(s) 93
BsmAI GTCTC 4 cut(s) 424, 558, 774, 980
BsmBI CGTCTC 1 cut(s) 424
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 4 cut(s) 203, 291, 388, 583
Bso31I GGTCTC 1 cut(s) 774
Bsp1286I GDGCHC 2 cut(s) 111, 873
Bsp143I GATC 1 cut(s) 270
Bsp19I CCATGG 1 cut(s) 383
BspACI CCGC 2 cut(s) 493, 991
BspANI GGCC 4 cut(s) 203, 291, 388, 583
BspCNI CTCAG 6 cut(s) 196, 220, 382, 441, 619, 878
BspLI GGNNCC 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 558
BspTNI GGTCTC 1 cut(s) 774
BsrBI CCGCTC 1 cut(s) 493
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 5 cut(s) 102, 192, 383, 880, 980
BssMI GATC 1 cut(s) 270
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 4 cut(s) 102, 192, 383, 980
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 2 cut(s) 359, 882
Bst4CI ACNGT 2 cut(s) 331, 733
Bst6I CTCTTC 2 cut(s) 612, 937
BstAPI GCANNNNNTGC 1 cut(s) 26
BstBAI YACGTR 1 cut(s) 475
BstC8I GCNNGC 1 cut(s) 280
BstDEI CTNAG 7 cut(s) 183, 207, 390, 395, 428, 627, 865
BstDSI CCRYGG 1 cut(s) 383
BstF5I GGATG 3 cut(s) 168, 259, 367
BstKTI GATC 1 cut(s) 273
BstMAI GTCTC 4 cut(s) 424, 558, 774, 980
BstMBI GATC 1 cut(s) 270
BstMWI GCNNNNNNNGC 5 cut(s) 26, 125, 288, 632, 706
BstNI CCWGG 2 cut(s) 359, 882
BstSCI CCNGG 2 cut(s) 357, 880
BstSFI CTRYAG 1 cut(s) 729
BstSLI GKGCMC 1 cut(s) 873
BstSNI TACGTA 1 cut(s) 475
BstXI CCANNNNNNTGG 2 cut(s) 211, 902
BsuI GTATCC 1 cut(s) 958
BsuRI GGCC 4 cut(s) 203, 291, 388, 583
BtgI CCRYGG 1 cut(s) 383
BtsCI GGATG 3 cut(s) 168, 259, 367
BtsIMutI CAGTG 3 cut(s) 20, 294, 873
BveI ACCTGC 1 cut(s) 558
Cac8I GCNNGC 1 cut(s) 280
CseI GACGC 1 cut(s) 79
Csp6I GTAC 2 cut(s) 403, 779
CviAII CATG 2 cut(s) 384, 445
CviQI GTAC 2 cut(s) 403, 779
DdeI CTNAG 7 cut(s) 183, 207, 390, 395, 428, 627, 865
DpnI GATC 1 cut(s) 272
DpnII GATC 1 cut(s) 270
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 2 cut(s) 612, 937
EarI CTCTTC 2 cut(s) 612, 937
Ecl136II GAGCTC 1 cut(s) 109
Eco105I TACGTA 1 cut(s) 475
Eco130I CCWWGG 4 cut(s) 102, 192, 383, 980
Eco147I AGGCCT 1 cut(s) 583
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 774
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 636
EcoICRI GAGCTC 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 2 cut(s) 357, 880
EcoT14I CCWWGG 4 cut(s) 102, 192, 383, 980
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 4 cut(s) 102, 192, 383, 980
Esp3I CGTCTC 1 cut(s) 424
FaeI CATG 2 cut(s) 387, 448
FatI CATG 2 cut(s) 383, 444
FauI CCCGC 1 cut(s) 486
Fnu4HI GCNGC 1 cut(s) 991
FokI GGATG 3 cut(s) 155, 266, 374
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 991
GluI GCNGC 1 cut(s) 991
HaeIII GGCC 4 cut(s) 203, 291, 388, 583
HapII CCGG 1 cut(s) 651
HgaI GACGC 1 cut(s) 79
Hin1II CATG 2 cut(s) 387, 448
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 5 cut(s) 83, 160, 221, 338, 661
HpaII CCGG 1 cut(s) 651
HphI GGTGA 5 cut(s) 17, 241, 433, 508, 705
Hpy166II GTNNAC 3 cut(s) 189, 472, 547
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 1 cut(s) 149
Hpy8I GTNNAC 3 cut(s) 189, 472, 547
Hpy99I CGWCG 2 cut(s) 95, 419
HpyAV CCTTC 3 cut(s) 313, 377, 720
HpyCH4III ACNGT 2 cut(s) 331, 733
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 7 cut(s) 20, 257, 553, 602, 805, 834, 851
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 125, 288, 632, 706
HpyF3I CTNAG 7 cut(s) 183, 207, 390, 395, 428, 627, 865
HpySE526I ACGT 1 cut(s) 474
Hsp92II CATG 2 cut(s) 387, 448
Kzo9I GATC 1 cut(s) 270
LmnI GCTCC 1 cut(s) 106
LweI GCATC 2 cut(s) 106, 244
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 2 cut(s) 658, 984
MalI GATC 1 cut(s) 272
MbiI CCGCTC 1 cut(s) 493
MboI GATC 1 cut(s) 270
MboII GAAGA 3 cut(s) 230, 629, 954
MhlI GDGCHC 2 cut(s) 111, 873
MlsI TGGCCA 1 cut(s) 203
MluNI TGGCCA 1 cut(s) 203
MlyI GAGTC 2 cut(s) 347, 655
MnlI CCTC 9 cut(s) 133, 145, 184, 302, 392, 401, 531, 759, 874
Mox20I TGGCCA 1 cut(s) 203
MscI TGGCCA 1 cut(s) 203
MseI TTAA 3 cut(s) 14, 123, 786
MslI CAYNNNNRTG 1 cut(s) 915
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 211
MspI CCGG 1 cut(s) 651
MspR9I CCNGG 2 cut(s) 359, 882
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 2 cut(s) 359, 882
MwoI GCNNNNNNNGC 5 cut(s) 26, 125, 288, 632, 706
NcoI CCATGG 1 cut(s) 383
NdeII GATC 1 cut(s) 270
NlaIII CATG 2 cut(s) 387, 448
NlaIV GGNNCC 1 cut(s) 412
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 2 cut(s) 658, 984
PceI AGGCCT 1 cut(s) 583
PctI GAATGC 1 cut(s) 31
PfeI GAWTC 3 cut(s) 83, 160, 221
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 1 cut(s) 992
PleI GAGTC 2 cut(s) 346, 655
PpsI GAGTC 2 cut(s) 346, 655
Ppu21I YACGTR 1 cut(s) 475
Psp124BI GAGCTC 1 cut(s) 111
Psp6I CCWGG 2 cut(s) 357, 880
PspGI CCWGG 2 cut(s) 357, 880
PspN4I GGNNCC 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 211
RsaI GTAC 2 cut(s) 404, 780
RsaNI GTAC 2 cut(s) 403, 779
RseI CAYNNNNRTG 1 cut(s) 915
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 3 cut(s) 14, 123, 786
SatI GCNGC 1 cut(s) 991
Sau3AI GATC 1 cut(s) 270
SchI GAGTC 2 cut(s) 347, 655
ScrFI CCNGG 2 cut(s) 359, 882
SduI GDGCHC 2 cut(s) 111, 873
SfaNI GCATC 2 cut(s) 106, 244
SfcI CTRYAG 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
SnaBI TACGTA 1 cut(s) 475
SseBI AGGCCT 1 cut(s) 583
SsiI CCGC 2 cut(s) 493, 991
SstI GAGCTC 1 cut(s) 111
StuI AGGCCT 1 cut(s) 583
StyD4I CCNGG 2 cut(s) 357, 880
StyI CCWWGG 4 cut(s) 102, 192, 383, 980
TaaI ACNGT 2 cut(s) 331, 733
TaiI ACGT 1 cut(s) 477
TaqI TCGA 3 cut(s) 449, 930, 940
TauI GCSGC 1 cut(s) 993
TfiI GAWTC 3 cut(s) 83, 160, 221
Tru1I TTAA 3 cut(s) 14, 123, 786
Tru9I TTAA 3 cut(s) 14, 123, 786
TscAI CASTG 3 cut(s) 27, 301, 873
TseFI GTSAC 2 cut(s) 658, 984
Tsp45I GTSAC 2 cut(s) 658, 984
TspDTI ATGAA 3 cut(s) 96, 359, 624
TspGWI ACGGA 3 cut(s) 296, 403, 1011
TspRI CASTG 3 cut(s) 27, 301, 873
XapI RAATTY 5 cut(s) 42, 131, 461, 808, 837
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.